Article(id=1304406844189995890, tenantId=1146029695717560320, journalId=1302319053441957962, issueId=1304406828071281069, articleNumber=null, orderNo=null, doi=10.7501/j.issn.0253-2670.2026.02.024, pmid=null, cstr=null, oa=null, hot=0, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1759334400000, receivedDateStr=2025-10-02, revisedDate=null, revisedDateStr=null, acceptedDate=null, acceptedDateStr=null, onlineDate=1788924426362, onlineDateStr=2026-09-09, pubDate=null, pubDateStr=null, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1788924426362, onlineIssueDateStr=2026-09-09, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1788924426362, creator=13701087609, updateTime=1788924426362, updator=13701087609, issue=Issue{id=1304406828071281069, tenantId=1146029695717560320, journalId=1302319053441957962, year='2026', volume='57', issue='2', pageStart='393', pageEnd='788', issueExtLink='null', onlineDate='null', pubDate='1769529600000', pubDateStr='2026-01-28', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1788924422518, creator='13701087609', updateTime=1788924652596, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1304407793138688830, tenantId=1146029695717560320, journalId=1302319053441957962, issueId=1304406828071281069, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1304407793138688831, tenantId=1146029695717560320, journalId=1302319053441957962, issueId=1304406828071281069, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=652, endPage=664, ext={EN=ArticleExt(id=1304406844684923764, articleId=1304406844189995890, tenantId=1146029695717560320, journalId=1302319053441957962, language=EN, title=Quality evaluation and antioxidant activity of different varieties of Mume Flos based on fingerprint combined with chemical pattern recognition, columnId=null, journalTitle=Chinese Traditional and Herbal Drugs, columnName=null, runingTitle=null, highlight=null, articleAbstract=Objective To evaluate the quality and differences among different varieties of Mume Flos by analyzing their fingerprints using chemical pattern recognition techniques, combined with quantitative analysis of characteristic components and antioxidant activity assays. Methods Ultra-high performance liquid chromatography (UPLC) was used to establish the fingerprints of different varieties of Mume Flos, combined with the chemical pattern recognition technology to explore the differences between different varieties of Mume Flos, screened for the characteristic chemical components that lead to the differences between different varieties of Mume Flos, and quantitatively analyzed them. The antioxidant activity of different varieties of Mume Flos was detected by DPPH free radical scavenging method. Results A total of 22 common peaks were identified in the fingerprints of 33 batches of Mume Flos.The similarity within the varietal group was above 0.869, while the similarity between the varietal groups was relatively low. Hierarchical cluster analysis (HCA), principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA) were used to classify the 33 batches of Mume Flos into two major groups and three subgroups, among which the green calyx group could be distinguished from the rest of the samples. The combination of OPLS-DA indicated that 12 components, including chlorogenic acid, rutin, hyperoside, isoquercitrin, isochlorogenic acid B, quercitrin, and naringenin, might be the characteristic markers leading to the differences in different varieties of Mume Flos. The results of the content determination showed that the samples from the green calyx variety group were dominant in the content of the components except naringenin. The results of antioxidant activity showed that the antioxidant capacity of green calyx variety group, the vermilion variety group, and DHH were relatively higher, and the antioxidant activities of various varieties of Mume Flos had strong correlations with chlorogenic acid, hyperoside, isoquercitrin, isochlorogenic acid B. Conclusion The content of components and antioxidant activity of different varieties of Mume Flos have some variability, among which the green calyx variety group is relatively stable. These results provide data support for exploring the variability of different Mume Flos varieties and the preference of “green calyx” for Mume Flos., authors=WU Rui, CHENG Qingbing, JIN Fenghua, LIU Yi, XU Liang, WANG Wei, ZHANG Wei, LI Guoqiang, OU Jinmei, authorsList=WU Rui, CHENG Qingbing, JIN Fenghua, LIU Yi, XU Liang, WANG Wei, ZHANG Wei, LI Guoqiang, OU Jinmei, authorCompany=null, correspAuthors=null, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1304406844416488307, articleId=1304406844189995890, tenantId=1146029695717560320, journalId=1302319053441957962, language=CN, title=基于指纹图谱结合化学模式识别评价不同品种梅花的质量及抗氧化活性, columnId=1304140203967410414, journalTitle=中草药, columnName=药材与资源, runingTitle=null, highlight=null, articleAbstract=目的 利用化学模式识别技术分析不同品种梅花Mume Flos指纹图谱,结合含量测定和抗氧化能力测定评价不同品种梅花的质量和差异性。方法 采用超高效液相色谱法(UPLC)建立不同品种梅花的指纹图谱,结合化学模式识别技术探讨不同品种梅花之间的差异性,筛选导致不同品种梅花差异的特征化学成分,并定量分析;采用DPPH自由基清除法检测不同品种梅花的抗氧化活性。结果 33批梅花指纹图谱共有22个共有峰,品种群内相似度在0.869以上,而品种群间相似度较低。聚类分析(hierarchical cluster analysis,HCA)、主成分分析(principal component analysis,PCA)及偏最小二乘判别分析(orthogonal partial least squares discriminant analysis,OPLS-DA)可将33批梅花分为2大类3小类,其中绿萼品种群可与其余样品区分。结合OPLS-DA表明,绿原酸、芦丁、金丝桃苷、异槲皮苷、异绿原酸B、槲皮苷、柚皮素等12个成分可能是导致不同品种梅花差异的特征标志物。含量测定结果表明,除柚皮素外,绿萼品种群样品在成分含量上占优势。抗氧化活性结果表明,绿萼品种群、朱砂品种群及DHH的抗氧化能力相对较强,各品种梅花抗氧化活性与绿原酸、金丝桃苷、异槲皮苷、异绿原酸B具有较强相关性。结论 不同品种梅花的成分含量及抗氧化活性具有一定差异性,其中绿萼品种群相对稳定。为探究不同品种梅花的差异性及梅花以“绿萼”为好提供数据支撑。, authors=吴瑞1, 程庆兵2, 金凤华2, 刘毅1, 徐良1, 王玮2, 张伟1,3, 李国强1, 欧金梅1,3,4, authorsList=吴瑞, 程庆兵, 金凤华, 刘毅, 徐良, 王玮, 张伟, 李国强, 欧金梅, authorCompany=1 安徽中医药大学药学院, 安徽 合肥 230012;
2 合肥市食品药品检验中心, 安徽 合肥 230012;
3 中药饮片制造新技术安徽省重点实验室, 安徽 合肥 230012;
4 道地药材国家重点实验室, 北京 100700, correspAuthors=李国强, authorNote=吴瑞: 吴瑞,女,硕士研究生,研究方向为中药资源与质量评价。E-mail: 3360940473@qq.com, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=axQeHy75yN5q47Uj/YRpOA==, pdfFileSize=2473811, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=名贵中药资源可持续利用能力建设项目 (2060302); 安徽道地主产药材药效物质基础研究 (RZ2100000757); 安徽省高校科研项目 (2023AH050787); 安徽中医药大学人才项目 (2021rcyb011))}, authors=null, keywords=[Keyword(id=1304406844894638965, tenantId=1146029695717560320, journalId=1302319053441957962, articleId=1304406844189995890, language=CN, orderNo=1, keyword=梅), Keyword(id=1304406844953359222, tenantId=1146029695717560320, journalId=1302319053441957962, articleId=1304406844189995890, language=CN, orderNo=2, keyword=梅花), Keyword(id=1304406845012079479, tenantId=1146029695717560320, journalId=1302319053441957962, articleId=1304406844189995890, language=CN, orderNo=3, keyword=指纹图谱), Keyword(id=1304406845070799736, tenantId=1146029695717560320, journalId=1302319053441957962, articleId=1304406844189995890, language=CN, orderNo=4, keyword=差异标志物), Keyword(id=1304406845163074425, tenantId=1146029695717560320, journalId=1302319053441957962, articleId=1304406844189995890, language=CN, orderNo=5, keyword=抗氧化活性), Keyword(id=1304406845221794682, tenantId=1146029695717560320, journalId=1302319053441957962, articleId=1304406844189995890, language=CN, orderNo=6, keyword=差异性), Keyword(id=1304406845284709243, tenantId=1146029695717560320, journalId=1302319053441957962, articleId=1304406844189995890, language=CN, orderNo=7, keyword=绿原酸), Keyword(id=1304406845343429500, tenantId=1146029695717560320, journalId=1302319053441957962, articleId=1304406844189995890, language=CN, orderNo=8, keyword=芦丁), Keyword(id=1304406845414732669, tenantId=1146029695717560320, journalId=1302319053441957962, articleId=1304406844189995890, language=CN, orderNo=9, keyword=金丝桃苷), Keyword(id=1304406845473452926, tenantId=1146029695717560320, journalId=1302319053441957962, articleId=1304406844189995890, language=CN, orderNo=10, keyword=异槲皮苷), Keyword(id=1304406845536367487, tenantId=1146029695717560320, journalId=1302319053441957962, articleId=1304406844189995890, language=CN, orderNo=11, keyword=异绿原酸B), Keyword(id=1304406845595087744, tenantId=1146029695717560320, journalId=1302319053441957962, articleId=1304406844189995890, language=CN, orderNo=12, keyword=槲皮苷), Keyword(id=1304406845662196609, tenantId=1146029695717560320, journalId=1302319053441957962, articleId=1304406844189995890, language=CN, orderNo=13, keyword=柚皮素), Keyword(id=1304406845754471298, tenantId=1146029695717560320, journalId=1302319053441957962, articleId=1304406844189995890, language=EN, orderNo=1, keyword=Prunus mume(Sieb.) 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中草药 |药材与资源 2026 , 57 (2) : 652 -664
基于指纹图谱结合化学模式识别评价不同品种梅花的质量及抗氧化活性
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吴瑞1, 程庆兵2, 金凤华2, 刘毅1, 徐良1, 王玮2, 张伟1,3, 李国强1, 欧金梅1,3,4
作者信息
    1 安徽中医药大学药学院, 安徽 合肥 230012;
    2 合肥市食品药品检验中心, 安徽 合肥 230012;
    3 中药饮片制造新技术安徽省重点实验室, 安徽 合肥 230012;
    4 道地药材国家重点实验室, 北京 100700
通讯作者:
李国强
作者简介:
吴瑞: 吴瑞,女,硕士研究生,研究方向为中药资源与质量评价。E-mail: 3360940473@qq.com
Quality evaluation and antioxidant activity of different varieties of Mume Flos based on fingerprint combined with chemical pattern recognition
  • WU Rui, CHENG Qingbing, JIN Fenghua, LIU Yi, XU Liang, WANG Wei, ZHANG Wei, LI Guoqiang, OU Jinmei
  • Affiliations
    doi: 10.7501/j.issn.0253-2670.2026.02.024
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    目的 利用化学模式识别技术分析不同品种梅花Mume Flos指纹图谱,结合含量测定和抗氧化能力测定评价不同品种梅花的质量和差异性。方法 采用超高效液相色谱法(UPLC)建立不同品种梅花的指纹图谱,结合化学模式识别技术探讨不同品种梅花之间的差异性,筛选导致不同品种梅花差异的特征化学成分,并定量分析;采用DPPH自由基清除法检测不同品种梅花的抗氧化活性。结果 33批梅花指纹图谱共有22个共有峰,品种群内相似度在0.869以上,而品种群间相似度较低。聚类分析(hierarchical cluster analysis,HCA)、主成分分析(principal component analysis,PCA)及偏最小二乘判别分析(orthogonal partial least squares discriminant analysis,OPLS-DA)可将33批梅花分为2大类3小类,其中绿萼品种群可与其余样品区分。结合OPLS-DA表明,绿原酸、芦丁、金丝桃苷、异槲皮苷、异绿原酸B、槲皮苷、柚皮素等12个成分可能是导致不同品种梅花差异的特征标志物。含量测定结果表明,除柚皮素外,绿萼品种群样品在成分含量上占优势。抗氧化活性结果表明,绿萼品种群、朱砂品种群及DHH的抗氧化能力相对较强,各品种梅花抗氧化活性与绿原酸、金丝桃苷、异槲皮苷、异绿原酸B具有较强相关性。结论 不同品种梅花的成分含量及抗氧化活性具有一定差异性,其中绿萼品种群相对稳定。为探究不同品种梅花的差异性及梅花以“绿萼”为好提供数据支撑。
    梅  /  梅花  /  指纹图谱  /  差异标志物  /  抗氧化活性  /  差异性  /  绿原酸  /  芦丁  /  金丝桃苷  /  异槲皮苷  /  异绿原酸B  /  槲皮苷  /  柚皮素
    Objective To evaluate the quality and differences among different varieties of Mume Flos by analyzing their fingerprints using chemical pattern recognition techniques, combined with quantitative analysis of characteristic components and antioxidant activity assays. Methods Ultra-high performance liquid chromatography (UPLC) was used to establish the fingerprints of different varieties of Mume Flos, combined with the chemical pattern recognition technology to explore the differences between different varieties of Mume Flos, screened for the characteristic chemical components that lead to the differences between different varieties of Mume Flos, and quantitatively analyzed them. The antioxidant activity of different varieties of Mume Flos was detected by DPPH free radical scavenging method. Results A total of 22 common peaks were identified in the fingerprints of 33 batches of Mume Flos.The similarity within the varietal group was above 0.869, while the similarity between the varietal groups was relatively low. Hierarchical cluster analysis (HCA), principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA) were used to classify the 33 batches of Mume Flos into two major groups and three subgroups, among which the green calyx group could be distinguished from the rest of the samples. The combination of OPLS-DA indicated that 12 components, including chlorogenic acid, rutin, hyperoside, isoquercitrin, isochlorogenic acid B, quercitrin, and naringenin, might be the characteristic markers leading to the differences in different varieties of Mume Flos. The results of the content determination showed that the samples from the green calyx variety group were dominant in the content of the components except naringenin. The results of antioxidant activity showed that the antioxidant capacity of green calyx variety group, the vermilion variety group, and DHH were relatively higher, and the antioxidant activities of various varieties of Mume Flos had strong correlations with chlorogenic acid, hyperoside, isoquercitrin, isochlorogenic acid B. Conclusion The content of components and antioxidant activity of different varieties of Mume Flos have some variability, among which the green calyx variety group is relatively stable. These results provide data support for exploring the variability of different Mume Flos varieties and the preference of “green calyx” for Mume Flos.
    Prunus mume(Sieb.) Sieb.et Zucc.  /  Mume Flos  /  fingerprint  /  differential markers  /  antioxidant activity  /  difference  /  chlorogenic acid  /  rutin  /  hyperoside  /  isoquercitrin  /  isochlorogenic acid B  /  quercitrin  /  naringenin
    吴瑞, 程庆兵, 金凤华, 刘毅, 徐良, 王玮, 张伟, 李国强, 欧金梅. 基于指纹图谱结合化学模式识别评价不同品种梅花的质量及抗氧化活性. 中草药, 2026 , 57 (2) : 652 -664 . DOI: 10.7501/j.issn.0253-2670.2026.02.024
    WU Rui, CHENG Qingbing, JIN Fenghua, LIU Yi, XU Liang, WANG Wei, ZHANG Wei, LI Guoqiang, OU Jinmei. Quality evaluation and antioxidant activity of different varieties of Mume Flos based on fingerprint combined with chemical pattern recognition[J]. Chinese Traditional and Herbal Drugs, 2026 , 57 (2) : 652 -664 . DOI: 10.7501/j.issn.0253-2670.2026.02.024

      名贵中药资源可持续利用能力建设项目 (2060302); 安徽道地主产药材药效物质基础研究 (RZ2100000757); 安徽省高校科研项目 (2023AH050787); 安徽中医药大学人才项目 (2021rcyb011)

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    陈安冉, 丁明君, 王保根. 梅花饮食文化探究[J]. 中国园林, 2020, 36(S1):33-35.
    唐桂梅, 黄国林, 曾斌, 等. 梅花种质资源研究进展[J]. 湖南农业科学, 2020(5):108-111.
    陈俊愉. 中国梅花的研究:Ⅱ.中国梅花的品种分类[J]. 园艺学报, 1962(S1):337-350.
    陈俊愉. 中国梅花品种分类新系统[J]. 北京林学院学报, 1981, 3(2):48-62.
    陈俊愉, 包满珠. 中国梅(Prunus mume Sieb. et Zucc.)变种(变型)与品种的分类学研究[J]. 北京林业大学学报, 1992(S4):1-6.
    陈俊愉. 中国梅花品种之种系、类、型分类检索表[J]. 中国园林, 1999, 15(1):64-65.
    陈俊愉. 中国梅花品种分类最新修正体系[J]. 北京林业大学学报, 1999, 21(2):1-6.
    陈俊愉, 陈瑞丹. 关于梅花Prunus mume的品种分类体系[J]. 园艺学报, 2007, 34(4):1055-1058.
    陈俊愉, 陈瑞丹. 中国梅花品种群分类新方案并论种间杂交起源品种群之发展优势[J]. 园艺学报, 2009, 36(5):693-700.
    张振宇, 严辉, 郭盛, 等. 梅花的本草考证[J]. 中国实验方剂学杂志, 2021, 27(10):117-125.
    清·赵学敏著. 闫冰等校注. 本草纲目拾遗[M]. 北京:中国中医药出版社, 1998:245-250.
    清·王士雄原著. 宋咏梅, 张传友点校. 随息居饮食谱[M]. 天津:天津科学技术出版社, 2003:44-45.
    《浙江药用植物志》编写组. 浙江药用植物志[M]. 杭州:浙江科学技术出版社, 1980:502-504.
    金荷仙, 陈俊愉, 金幼菊. 南京不同类型梅花品种香气成分的比较研究[J]. 园艺学报, 2005, 32(6):1139.
    Zhang T X, Bao F, Yang Y J, et al. A comparative analysis of floral scent compounds in intraspecific cultivars of Prunus mume with different Corolla colours[J]. Molecules, 2019, 25(1):145.
    Zhao C L, Guo W M, Chen J Y. Research advances in the flower color of Prunus mume[J]. J Beijing Fore Univ, 2004, 26:123-127.
    Zhang Q, Hao R J, Xu Z D, et al. Isolation and functional characterization of a R2R3-MYB regulator of Prunus mume anthocyanin biosynthetic pathway[J]. Plant Cell Tissue Organ Cult PCTOC, 2017, 131(3):417-429.
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    2026年第57卷第2期
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    2种不同金属材料的力学参数

    Family
    属数
    Number of
    genus
    种数
    Number of
    species
    占总种数比例
    Percentage of
    total species (%)

    Genus
    种数
    Number of
    species
    占总种数比例
    Percentage of total
    species (%)
    鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
    小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
    多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
    红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
    小菇属 Mycena 11 5.26
    光柄菇属 Pluteus 5 2.39
    红菇属 Russula 17 8.13
    栓菌属 Trametes 5 2.39
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