中草药

隔山牛皮消中2个新化学成分研究

PDF下载

张露元, 周晶, 娄红波, 普开亮, 李云璐, 陈超, 陈英飞, 徐笑宇, 沈勇

中草药 | 化学成分 2026,57(2): 400-404

收起

中草药 |化学成分 2026 , 57 (2) : 400 -404

隔山牛皮消中2个新化学成分研究

全屏

张露元, 周晶, 娄红波, 普开亮, 李云璐, 陈超, 陈英飞, 徐笑宇, 沈勇

作者信息

通讯作者:

徐笑宇

作者简介:

张露元: 张露元（2001—），女，硕士研究生，研究方向为药用植物资源化学开发利用。E-mail: 1483669890@qq.com 周晶: 周晶（1999—），男，硕士研究生，研究方向为药用植物资源化学开发利用。E-mail: 2320701463@qq.com

Two new trinorcycloartane triterpenoids from stems and leaves of Astragalus membranaceus

ZHANG Luyuan, ZHOU Jing, LOU Hongbo, PU Kailiang, LI Yunlu, CHEN Chao, CHEN Yingfei, XU Xiaoyu, SHEN Yong

Affiliations

doi: 10.7501/j.issn.0253-2670.2026.02.002

文章导航

摘要

收起

目的研究隔山牛皮消Cynanchum wilfordii的化学成分及其抗炎活性。方法采用硅胶、ODS和Sephadex LH-20等多种色谱分离技术开展化合物的分离纯化，运用HR-ESI-MS、ESI-MS、¹H-NMR、¹³C-NMR、HSQC、HMBC多种波谱技术鉴定化合物结构；采用Griess法研究化合物的抗炎活性。结果从隔山牛皮消块根中分离得到2个化合物，分别鉴定为(7'R,8'S)-dehydrodiconiferyl alcohol 9'-O-β-D-xylopyranoside（1）和3β-[(O-β-D-glucopyranosyloxy]-8β,14β-trihydroxycarda-5,20(22)-dienolide（2）。化合物1和2对NO生成的抑制率分别为55.16%和15.34%，化合物1的半数抑制浓度（median inhibition concentration，IC₅₀）为41.37 μmol/L。结论化合物1与2均为新化合物，分别属于木脂素和强心苷类，分别命名为隔山牛皮消素A和隔山牛皮消强心苷A。体外抗炎实验表明，化合物1具有一定的抗炎活性。

关键词

隔山牛皮消 / 木脂素 / 强心苷 / 抗炎活性 / 隔山牛皮消素A / 隔山牛皮消强心苷A

Abstract

收起

Objective To investigate the chemical constituents and their anti-inflammatory activity of Cynanchum wilfordii. Methods Various chromatographic techniques including silica gel, ODS, and Sephadex LH-20 column chromatography were employed for the isolation and purification of compounds. Their structures were elucidated through comprehensive spectroscopic analysis including HR-ESI-MS, ESI-MS, ¹H-NMR, ¹³C-NMR, HSQC, and HMBC. The anti-inflammatory activity of the compounds was evaluated using the Griess method. Results Two compounds were isolated and identified as (7'R,8'S)-dehydrodiconiferyl alcohol 9'-O-β-D-xylopyranoside (1) and 3β-[(O-β-D-glucopyranosyl)-oxy]-8β,14β-trihydroxycarda-5,20-(22)-dienolide (2). Compounds 1 and 2 inhibited NO production by 55.16% and 15.34%, respectively, with a median inhibition concentration (IC₅₀) of 41.37 μmol/L for compound 1. Conclusion Compounds 1 and 2 are both new compounds, belonging to lignan and cardenolide types, respectively. They have been assigned the names cynawilforin A (1) and wilforcardenoside A (2). In vitro anti-inflammatory assays indicated that compound 1 exhibits certain anti-inflammatory activity.

Key words

Cynanchum wilfordii(Maxim.) Hemsl / lignan / cardenolide / anti-inflammatory activity / cynawilforin A / wilforcardenoside A

引用本文

张露元, 周晶, 娄红波, 普开亮, 李云璐, 陈超, 陈英飞, 徐笑宇, 沈勇. 隔山牛皮消中2个新化学成分研究. 中草药, 2026 , 57 (2) : 400 -404 . DOI: 10.7501/j.issn.0253-2670.2026.02.002

ZHANG Luyuan, ZHOU Jing, LOU Hongbo, PU Kailiang, LI Yunlu, CHEN Chao, CHEN Yingfei, XU Xiaoyu, SHEN Yong. Two new trinorcycloartane triterpenoids from stems and leaves of Astragalus membranaceus[J]. Chinese Traditional and Herbal Drugs, 2026 , 57 (2) : 400 -404 . DOI: 10.7501/j.issn.0253-2670.2026.02.002

基金

收起

云南省高校服务重点产业科技项目 (FWCY-QYCT2024014); 建设面向南亚东南亚科技创新中心专项项目 (202403AP140013)

参考文献

收起

文献

收起

参考文献引证文献

排序方式：

Shibata S, Ogihara Y, Tokutake N, et al. Duclauxin, a metabolite of Penicillium duclauxi (Delacroix)[J]. Tetrahedron Lett, 1965(18):1287-1288.
Shahid H, Cai T, Wang Y Y, et al. Duclauxin derivatives from fungi and their biological activities[J]. Front Microbiol, 2021, 12:766440.
Wang M H, Yang L H, Feng L B, et al. Verruculosins A-B, new oligophenalenone dimers from the soft coral-derived fungus Talaromyces verruculosus[J]. Mar Drugs, 2019, 17(9):516.
Cao P, Yang J, Miao C P, et al. New duclauxamide from Penicillium manginii YIM PH30375 and structure revision of the duclauxin family[J]. Org Lett, 2015, 17(5):1146-1149.
Dramae A, Intaraudom C, Bunbamrung N, et al. Heptacyclic oligophenalenones from the soil fungus Talaromyces bacillisporus BCC17645[J]. Tetrahedron, 2020, 76(9):130980.
Wu B, Ohlendorf B, Oesker V, et al. Acetylcholinesterase inhibitors from a marine fungus Talaromyces sp. strain LF458[J]. Mar Biotechnol, 2015, 17(1):110-119.
Chaiyosang B, Kanokmedhakul K, Sanmanoch W, et al. Bioactive oxaphenalenone dimers from the fungus Talaromyces macrosporus KKU-1NK8[J]. Fitoterapia, 2019, 134:429-434.
Machado F P, Rodrigues I C, Georgopolou A, et al. New hybrid phenalenone dimer, highly conjugated dihydroxylated C₂₈ steroid and azaphilone from the culture extract of a marine sponge-associated fungus, Talaromyces pinophilus KUFA 1767[J]. Mar Drugs, 2023, 21(3):194.
Jiménez-Arreola B S, Aguilar-Ramírez E, Cano-Sánchez P, et al. Dimeric phenalenones from Talaromyces sp. (IQ-313) inhibit hPTP1B1-400:Insights into mechanistic kinetics from in vitro and in silico studies[J]. Bioorg Chem, 2020, 101:103893.
王龙, 金世宇. 篮状菌属蓝状菌组的两个中国新记录种[J]. 聊城大学学报:自然科学版, 2022, 35(3):56-61.
Suzuki T, Tanemura K, Horaguchi T, et al. Synthesis and structure of 8-hydroxy-6-methoxy-3,7-dimethyliso-chromane and its analogues[J]. Tetrahedron, 2006, 62(15):3739-3751.
Song F H, Dong Y F, Wei S Z, et al. New antibacterial secondary metabolites from a marine-derived Talaromyces sp. strain BTBU20213036[J]. Antibiotics, 2022, 11(2): 222.
Zang Y, Genta-Jouve G, Escargueil A E, et al. Antimicrobial oligophenalenone dimers from the soil fungus Talaromyces stipitatus[J]. J Nat Prod, 2016, 79(12):2991-2996.
Yang S W, Chan T M, Terracciano J, et al. A new hydrogenated azaphilone Sch 725680 from Aspergillus sp[J]. J Antibiot:Tokyo, 2006, 59(11):720-723.
Petersen L M, Hoeck C, Frisvad J C, et al. Dereplication guided discovery of secondary metabolites of mixed biosynthetic origin from Aspergillus aculeatu[J]. Molecules, 2014, 19(8):10898-10921.
Shao C L, Wang C Y, Wei M Y, et al. Two new benzaldehyde derivatives from mangrove endophytic fungus (No. ZZF 32)[J]. Chem Nat Compd, 2009, 45(6):779-781.
Lei L R, Gong L Q, Jin M Y, et al. Research advances in the structures and biological activities of secondary metabolites from Talaromyces[J]. Front Microbiol, 2022, 13:984801.

2026年第57卷第2期

PDF下载

引用本文

BibTeX

文章信息

doi: 10.7501/j.issn.0253-2670.2026.02.002

接收时间：2025-11-12
首发时间：2026-09-09

补充材料

相关文章

文章信息

作者

出版历史

收稿日期：2025-11-12

基金

作者信息

参考文献

分享链接

https://castjournals.cast.org.cn/joweb/zcy/CN/10.7501/j.issn.0253-2670.2026.02.002

分享至

全文二维码

扫描看全文

引用本文

BibTeX

本文的引用情况

2种不同金属材料的力学参数

科 Family	属数 Number of genus	种数 Number of species	占总种数比例 Percentage of total species (%)	属 Genus	种数 Number of species	占总种数比例 Percentage of total species (%)
鹅膏菌科Amanitaceae	2	11	5.26	鹅膏菌属 Amanita	10	4.78
小菇科 Mycenaceae	2	12	5.74	丝盖伞属 Inocybe	5	2.39
多孔菌科 Polyporaceae	8	14	6.70	蜡蘑属 Laccaria	5	2.39
红菇科 Russulaceae	3	23	11.00	小皮伞属 Marasmius	6	2.87
				小菇属 Mycena	11	5.26
				光柄菇属 Pluteus	5	2.39
				红菇属 Russula	17	8.13
				栓菌属 Trametes	5	2.39

关闭全屏

BibTeX
EndNote
RefWorks
TxT