中草药

白及多糖和甘草酸共稳定的青藤碱纳米乳的制备及其体外抗炎评价

PDF下载

中草药 | 药剂与工艺 2026,57(1): 95-108

收起

中草药 |药剂与工艺 2026 , 57 (1) : 95 -108

白及多糖和甘草酸共稳定的青藤碱纳米乳的制备及其体外抗炎评价

全屏

但武龙^1,2, 杨菁¹, 陈智勇^1,2, 陈滕^1,2, 俸婷婷^1,2, 朱月^1,2, 周英^1,2, 汪祖华^1,2

作者信息

通讯作者:

汪祖华

作者简介:

但武龙: 但武龙,男,硕士研究生,研究方向为纳米创新药物制剂开发。E-mail:ll2906291286@163.com

Preparation and in vitro anti-inflammatory evaluation of sinomenine nanoemulsion co-stabilized by Bletilla striata polysaccharide and glycyrrhizic acid

DAN Wulong, YANG Jing, CHEN Zhiyong, CHEN Teng, FENG Tingting, ZHU Yue, ZHOU Ying, WANG Zuhua

Affiliations

doi: 10.7501/j.issn.0253-2670.2026.01.011

文章导航

摘要

收起

目的制备白及多糖/甘草酸共稳定的青藤碱纳米乳（sinomenine nanoemulsion，Sin-NE），并系统表征其性质，考察其稳定性与释药行为，进而通过体外实验评价其抗炎效果。方法采用超声乳化法制备Sin-NE，观测其形貌、粒径、多分散指数（polydispersity index，PDI）、ζ电位，单因素实验和正交试验筛选最佳处方并进行处方验证，测量其pH值、浊度、黏度、包封率和载药量，进行稳定性考察，探究其体外释药行为。构建RAW264.7细胞炎症模型，验证Sin-NE对一氧化氮（nitric oxide，NO）、白细胞介素-6（interleukin-6，IL-6）、肿瘤坏死因子-α（tumor necrosis factor-α，TNF-α）的影响。结果 Sin-NE的最优处方为薏苡仁油用量3%，联合表面活性剂用量0.5%，白及多糖与甘草酸质量比1∶9，超声处理时间10 min，超声功率325 W。该处方所制备的Sin-NE平均粒径为（145.30±2.35）nm，ζ电位为（-39.51±0.43）mV，PDI为0.095±0.022，pH值为3.990±0.033。其包封率达到88.78%，载药量为26.14 mg/g。此外，Sin-NE在离心、储存、稀释及加热条件下均表现出良好的稳定性，并具备缓释特性，在体外能显著抑制炎症细胞中NO的释放，有效降低炎症因子IL-6和TNF-α的水平。结论 Sin-NE显著提高了青藤碱的生物利用度和抗炎活性，为新型中药纳米乳制剂的开发提供了实验依据。

关键词

白及多糖 / 青藤碱 / 甘草酸 / 纳米乳 / 抗炎活性 / 超声乳化法 / 白细胞介素-6 / 肿瘤坏死因子-α

Abstract

收起

Objective To prepare sinomenine nanoemulsion (Sin-NE) co-stabilized by Bletilla striata polysaccharide and glycyrrhizic acid, systematically characterize its properties, investigate its stability and drug release behavior, and further evaluate its anti-inflammatory effects through in vitro experiments. Methods The Sin-NE was prepared using the ultrasonic emulsification method. Its morphology, particle size, polydispersity index (PDI), and ζ potential were observed and determined. Optimal formulation parameters were screened via single-factor and orthogonal experiments, followed by formulation validation. pH value, turbidity, viscosity, encapsulation efficiency, and drug loading capacity were measured. Stability was investigated under centrifugation, storage, dilution, and heating conditions was evaluated, and in vitro drug release behavior was investigated. A RAW264.7 cell inflammation model was established to validate the effects of the Sin-NE on nitric oxide (NO), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α). Results The optimized formulation comprised 3% Coicis Semen oil, 0.5% combined emulsifiers, a 1∶9 mass ratio of Bletilla striata polysaccharide to glycyrrhizic acid, 10 min of ultrasonic treatment, and 325 W ultrasonic power. The resulting Sin-NE exhibited an average particle size of (145.30 ±2.35) nm, ζ potential of (-39.51 ±0.43) mV, PDI of 0.095 ±0.022, and pH value of 3.990 ±0.033. Encapsulation efficiency and drug loading capacity reached 88.78% and 26.14 mg/g, respectively. The nanoemulsion demonstrated excellent stability under centrifugation, storage, dilution, and heating conditions and displayed sustained-release characteristics. In vitro, it significantly inhibited NO release and effectively reduced IL-6 and TNF-α levels in inflamed cells. Conclusion The Sin-NE significantly enhances sinomenine’s bioavailability and anti-inflammatory activity, providing experimental evidence for developing novel traditional Chinese medicine nanoemulsion formulations.

Key words

Bletilla striata polysaccharide / sinomenine / glycyrrhizic acid / nanoemulsion / anti-inflammatory activity / ultrasonic emulsification method / interleukin-6 / tumor necrosis factor-α

引用本文

但武龙, 杨菁, 陈智勇, 陈滕, 俸婷婷, 朱月, 周英, 汪祖华. 白及多糖和甘草酸共稳定的青藤碱纳米乳的制备及其体外抗炎评价. 中草药, 2026 , 57 (1) : 95 -108 . DOI: 10.7501/j.issn.0253-2670.2026.01.011

DAN Wulong, YANG Jing, CHEN Zhiyong, CHEN Teng, FENG Tingting, ZHU Yue, ZHOU Ying, WANG Zuhua. Preparation and in vitro anti-inflammatory evaluation of sinomenine nanoemulsion co-stabilized by Bletilla striata polysaccharide and glycyrrhizic acid[J]. Chinese Traditional and Herbal Drugs, 2026 , 57 (1) : 95 -108 . DOI: 10.7501/j.issn.0253-2670.2026.01.011

基金

收起

国家自然科学基金项目 (82360852); 贵州省科技计划项目 (黔科合基础-ZK[2023]重点046); 贵州省现代中药创制全省重点实验室 (黔科合平台[2025]019); 贵州中医药大学纳米药物技术研究中心 (贵中医ZX合字[2024]070)

参考文献

收起

文献

收起

参考文献引证文献

排序方式：

徐易琳,黄莉,周莉莉,等.青藤碱药理作用及临床应用的研究进展[J]. 华西药学杂志, 2024, 39(2):209-215.
罗艳群,沈小兰,蔡三金,等.青风藤化学成分和药理作用的研究进展及其质量标志物(Q-Marker)的预测分析[J]. 中草药, 2022, 53(3):898-911.
周莹莹,刘宇灵,林龙飞,等.青藤碱及其新剂型治疗类风湿性关节炎的研究进展[J]. 中草药, 2021, 52(24):7709-7717.
Song L Q, Liu D, Zhao Y, et al. Sinomenine inhibits breast cancer cell invasion and migration by suppressing NF-κB activation mediated by IL-4/miR-324-5p/CUEDC2 axis[J]. Biochem Biophys Res Commun, 2015, 464(3):705-710.
Zeng M Y, Tong Q Y. Anti-inflammation effects of sinomenine on macrophages through suppressing activated TLR4/NF-κB signaling pathway[J]. Curr Med Sci, 2020, 40(1):130-137.
王金凤,王枫,刘杰,等.青藤碱通过JAK/STAT通路调控巨噬细胞极化改善胶原诱导性关节炎大鼠炎症和骨破坏的作用研究[J]. 中药药理与临床, 2025, 41(5):27-33.
Zhang B, Zhang H J, Luo H, et al. Sinomenine can promote the proliferation and differentiation of osteoblasts by regulating the Akt/Runx2 signaling pathway in MC3T3-E1 cells[J]. Pharmazie, 2019, 74(12):747-750.
邹飏,李浩,尚江荫子,等.青藤碱对前成骨细胞增殖、分化的影响及可能的作用机制[J]. 江西医药,2018, 53(2):119-121.
张晓强,李孟芳,李萍.正清风痛宁缓释片联合苓液汤治疗干燥综合征41例临床观察[J]. 新中医, 2014,46(2):117-119.
冯皓盈,王东凯,代英辉.青藤碱治疗类风湿关节炎新剂型研究进展[J]. 中国药剂学杂志:网络版, 2025,23(2):109-119.
张游,唐旖雯,彭玉婷,等.青藤碱治疗类风湿关节炎的作用机制研究进展[J]. 广西中医药大学学报, 2025,28(3):59-63.
张继芬,董芳明,刘川,等.药物纳米晶自稳定Pickering乳液给药系统研究的关键问题[J]. 药学学报, 2024, 59(6):1582-1592.
阮慧妮,丁文雅,杨旭,等.中药皂苷类成分纳米制剂的研究进展[J]. 中国医院药学杂志, 2025, 45(7):827-833.
马永强,牛绩超,尤婷婷,等. Pickering乳液的稳定性研究及其在食品领域的应用进展[J]. 食品工业科技,2023, 44(23):376-386.
Chachra D, Coote J G, Parton R, et al. Haemolytic and cytotoxic activities of the Tween 80-extracted putative haemolysin of Pasteurella multocida B:2[J]. Vet Microbiol, 2011, 150(3/4):331-337.
陈悦,官智超,樊文洁,等.以甘草酸为稳定剂的白藜芦醇纳米混悬剂的制备及评价[J]. 广东药科大学学报, 2025, 41(4):16-22.
周晶晶,周洁,窦霞,等.不同炮制方法对甘草化学成分及药理作用的影响研究[J]. 中华中医药学刊, 2024,42(5):244-249.
Wu Y F, Guo Y L, Huang T R, et al. Licorice flavonoid alleviates gastric ulcers by producing changes in gut microbiota and promoting mucus cell regeneration[J]. Biomed Pharmacother, 2023, 169:115868.
沈晓静,秦宇,华宗,等.白及多糖的提取纯化、结构鉴定及生物活性研究进展[J]. 中华中医药学刊, 2024,42(5):225-232.
Wang Y R, Han S W, Li R F, et al. Structural characterization and immunological activity of polysaccharides from the tuber of Bletilla striata[J]. Int J Biol Macromol, 2019, 122:628-635.
冯永波,高景,周忠海,等.白及多糖对巨噬细胞M1型极化的调节作用及机制研究[J]. 药物评价研究,2024, 47(11):2525-2532.
Chen Z Y, Cheng L Z, He Y C, et al. Extraction,characterization, utilization as wound dressing and drug delivery of Bletilla striata polysaccharide:A review[J]. Int J Biol Macromol, 2018, 120:2076-2085.
刘家燕.解毒散结方对肝热血瘀型肝癌TACE术后疗效的影响及其基于数据挖掘的组方规律研究[D]. 南宁:广西中医药大学, 2025.
Tan S F, Masoumi H R F, Karjiban R A, et al. Ultrasonic emulsification of parenteral valproic acid-loaded nanoemulsion with response surface methodology and evaluation of its stability[J]. Ultrason Sonochem, 2016,29:299-308.
Devendiran D K, Amirtham V A. A review on preparation,characterization, properties and applications of nanofluids[J]. Renew Sustain Energy Rev, 2016, 60:21-40.
Blaak J, Staib P. The relation of pH and skin cleansing[J]. Curr Probl Dermatol, 2018, 54:132-142.
Zhu Y, Chen T, Feng T T, et al. Fabrication and biological activities of all-in-one composite nanoemulsion based on Blumea balsamifera oil-tea tree oil[J]. Molecules, 2023,28(15):5889.
Hou P P, Pu F L, Zou H Y, et al. Whey protein stabilized nanoemulsion:A potential delivery system for ginsenoside Rg3 whey protein stabilized nanoemulsion:Potential Rg3delivery system[J]. Food Biosci, 2019, 31:100427.
李伟宏,张涛,高娟,等.白藜芦醇pH敏感介孔二氧化硅纳米粒的制备、表征和体外抗肿瘤活性[J]. 现代药物与临床, 2025, 40(8):1934-1942.
赵海燕,陆金玲,鲁妍,等.光甘草定烟酰胺纳米乳的制剂评价与透皮吸收[J]. 中南民族大学学报:自然科学版, 2024, 43(4):468-473.
郑海新.大黄酚-Pluronic F127/甘草酸混合胶束的制备、药动学及体外抗肿瘤初步研究[D]. 张家口:河北北方学院, 2025.
Guo Y L, Zhang X P, Wang X H, et al. Nanoemulsions stable against Ostwald ripening[J]. Langmuir, 2024,40(2):1364-1372.
魏征.小檗碱复合磷脂醇质体水凝胶的制备及其治疗小鼠乳腺炎的效果评估[D]. 哈尔滨:东北农业大学,2025.
Liu W W, Zhang Y J, Zhu W N, et al. Sinomenine inhibits the progression of rheumatoid arthritis by regulating the secretion of inflammatory cytokines and monocyte/macrophage subsets[J]. Front Immunol, 2018, 9:2228.
Wang Y J, Wang L L, Luo R L, et al. Glycyrrhizic acid against Mycoplasma gallisepticum-induced inflammation and apoptosis through suppressing the MAPK pathway in chickens[J]. J Agric Food Chem, 2022, 70(6):1996-2009.
Yue L, Wang W, Wang Y, et al. Bletilla striata polysaccharide inhibits angiotensin II-induced ROS and inflammation via NOX4 and TLR2 pathways[J]. Int J Biol Macromol, 2016, 89:376-388.
Zhang C F, Zhang W F, Shi R Y, et al. Coix lachryma-jobi extract ameliorates inflammation and oxidative stress in a complete Freund’s adjuvant-induced rheumatoid arthritis model[J]. Pharm Biol, 2019, 57(1):792-798.

2026年第57卷第1期

PDF下载

引用本文

BibTeX

文章信息

doi: 10.7501/j.issn.0253-2670.2026.01.011

接收时间：2025-10-09
首发时间：2026-09-09

补充材料

相关文章

文章信息

作者

出版历史

收稿日期：2025-10-09

基金

作者信息

参考文献

分享链接

https://castjournals.cast.org.cn/joweb/zcy/CN/10.7501/j.issn.0253-2670.2026.01.011

分享至

全文二维码

扫描看全文

引用本文

BibTeX

本文的引用情况

2种不同金属材料的力学参数

科 Family	属数 Number of genus	种数 Number of species	占总种数比例 Percentage of total species (%)	属 Genus	种数 Number of species	占总种数比例 Percentage of total species (%)
鹅膏菌科Amanitaceae	2	11	5.26	鹅膏菌属 Amanita	10	4.78
小菇科 Mycenaceae	2	12	5.74	丝盖伞属 Inocybe	5	2.39
多孔菌科 Polyporaceae	8	14	6.70	蜡蘑属 Laccaria	5	2.39
红菇科 Russulaceae	3	23	11.00	小皮伞属 Marasmius	6	2.87
				小菇属 Mycena	11	5.26
				光柄菇属 Pluteus	5	2.39
				红菇属 Russula	17	8.13
				栓菌属 Trametes	5	2.39

关闭全屏

BibTeX
EndNote
RefWorks
TxT