首页 期刊 论文 学科刊群 资讯 加盟 关于
EN
image
Article(id=1222469882294297305, tenantId=1146029695717560320, journalId=1189982191388893191, issueId=1222469875008790921, articleNumber=null, orderNo=null, doi=10.16438/j.0513-4870.2019-0407, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=research-article, receivedDate=1558627200000, receivedDateStr=2019-05-24, revisedDate=1562169600000, revisedDateStr=2019-07-04, acceptedDate=null, acceptedDateStr=null, onlineDate=1769389132330, onlineDateStr=2026-01-26, pubDate=1573488000000, pubDateStr=2019-11-12, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1769389132330, onlineIssueDateStr=2026-01-26, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1769389132330, creator=13701087609, updateTime=1769389132330, updator=13701087609, issue=Issue{id=1222469875008790921, tenantId=1146029695717560320, journalId=1189982191388893191, year='2019', volume='54', issue='11', pageStart='1881', pageEnd='2140', issueExtLink='null', onlineDate='null', pubDate='1573488000000', pubDateStr='2019-11-12', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1769389130593, creator='13701087609', updateTime=1769389577080, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1222471747778109959, tenantId=1146029695717560320, journalId=1189982191388893191, issueId=1222469875008790921, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1222471747778109960, tenantId=1146029695717560320, journalId=1189982191388893191, issueId=1222469875008790921, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=2059, endPage=2063, ext={EN=ArticleExt(id=1222469882927637257, articleId=1222469882294297305, tenantId=1146029695717560320, journalId=1189982191388893191, language=EN, title=Fingerprint analysis and multi-component determination of Leonurus japonicus by high performance liquid chromatography, columnId=1218263312677130587, journalTitle=Acta Pharmaceutica Sinica, columnName=Special Reports, runingTitle=null, highlight=null, articleAbstract=

An HPLC fingerprint and multi-component determination method of Leonurus japonicus was established for comprehensive evaluation and quality control of Leonurus japonicus. The sample was incubated in 70% ethanol in a water bath for 2 h, and the extract was analyzed by HPLC using Kromasil C18 column (250 mm×4.6 mm, 5 μm). The mobile phase consisted of acetonitrile-0.1% formic acid with gradient elution. The flow rate was 1.0 mL·min-1. The temperature of column was 30℃. The detection wavelength was 280 nm. HPLC fingerprint of characteristic components of Leonurus japonicus was established. There were 12 common peaks among 25 batches of samples, and 5 of them were identified and determined. Syringic acid, leonurine hydrochloride, rutin, hyperoside or isoquercitrin showed a good linearity in the ranges of 0.426 1-85.22 ng (r=0.999 9), 7.948-1 590 ng (r=0.999 3), 10.20-2 040 ng (r=1.000 0), 2.018-403.6 ng (r=0.999 9), or 8.704-1 741 ng (r=0.999 9), respectively. The average recoveries were 99.0%, 97.6%, 97.4%, 96.9% and 98.5% with RSD of 1.1%, 1.8%, 1.4%, 1.5% and 1.3%, respectively. The HPLC characteristic fingerprint of Leonurus japonicus was specific, and this method can simultaneously determine the content of 5 components.

, authors=null, authorsList=Chen SUN, Zhe-yu XIE, Yong-jiang WU, authorCompany=null, correspAuthors=Yong-jiang WU, authorNote=null, correspAuthorsNote=null, copyrightStatement=Copyright ©2019 Acta Pharmaceutica Sinica. All rights reserved., copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1222469886169834379, articleId=1222469882294297305, tenantId=1146029695717560320, journalId=1189982191388893191, language=CN, title=益母草HPLC指纹图谱及多成分含量测定方法研究, columnId=1190335348896011050, journalTitle=药学学报, columnName=研究论文, runingTitle=null, highlight=null, articleAbstract=

建立益母草的HPLC指纹图谱及多成分含量同时测定方法，为益母草质量综合评价和控制提供参考。益母草用70%乙醇水浴回流提取2 h，提取物采用HPLC法分析，色谱柱为Kromasil C18（250mm×4.6 mm，5 μm），流动相为乙腈-0.1%甲酸，梯度洗脱，流速为1.0 mL·min-1，柱温30℃，检测波长为280 nm。25批样品得到共有峰12个，确认了其中5个峰对应的化学成分。丁香酸、盐酸益母草碱、芦丁、金丝桃苷和异槲皮苷分别在进样量0.426 1~85.22ng（r=0.999 9）、7.948~1590 ng（r=0.999 3）、10.20~2040 ng（r=1.000 0）、2.018~403.6ng（r=0.999 9）、8.704~1741 ng（r=0.999 9）内呈良好的线性关系；平均回收率分别为99.0%、97.6%、97.4%、96.9%和98.5%，RSD分别为1.1%、1.8%、1.4%、1.5%和1.3%。该指纹图谱特征性明显，并可同时测定5个成分的含量。

, authors=null, authorsList=孙晨, 谢浙裕, 吴永江, authorCompany=null, correspAuthors=吴永江, authorNote=null, correspAuthorsNote=
*吴永江 ,Tel: 86-571-88208455, E-mail:
, copyrightStatement=版权所有©《药学学报》编辑部2019, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=95iULv4Kqmyt3a2hGukC5g==, magXml=rmcpLW4H7j8sCSLDQ6ON6g==, pdfUrl=null, pdf=pIcItai+kla4bsV2XoaOKA==, pdfFileSize=402880, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=YA8bcNVw//b2Vsgh7SOctA==, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=OibNl1pHmRt5h+WzYTO5Aw==, mapNumber=null, fund=null)}, authors=[Author(id=1222469886652179390, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1222469882294297305, orderNo=0, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1222469886790591433, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1222469882294297305, authorId=1222469886652179390, language=EN, stringName=Chen SUN, firstName=Chen, middleName=null, lastName=SUN, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, 2, address=1. College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China
2. Shaoxing Institute for Food and Drug Control, Shaoxing 312071, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1222469886920614867, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1222469882294297305, authorId=1222469886652179390, language=CN, stringName=孙晨, firstName=晨, middleName=null, lastName=孙, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, 2, address=1.浙江大学药学院, 浙江 杭州 310058
2.绍兴市食品药品检验研究院, 浙江 绍兴 312071, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1222469886396326820, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1222469882294297305, xref=null, ext=[AuthorCompanyExt(id=1222469886400521125, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1222469882294297305, companyId=1222469886396326820, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1. College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China), AuthorCompanyExt(id=1222469886408909734, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1222469882294297305, companyId=1222469886396326820, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1.浙江大学药学院, 浙江 杭州 310058)]), AuthorCompany(id=1222469886526350256, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1222469882294297305, xref=null, ext=[AuthorCompanyExt(id=1222469886534738865, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1222469882294297305, companyId=1222469886526350256, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2. Shaoxing Institute for Food and Drug Control, Shaoxing 312071, China), AuthorCompanyExt(id=1222469886538933170, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1222469882294297305, companyId=1222469886526350256, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2.绍兴市食品药品检验研究院, 浙江 绍兴 312071)])]), Author(id=1222469887046444002, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1222469882294297305, orderNo=1, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1222469887168078828, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1222469882294297305, authorId=1222469887046444002, language=EN, stringName=Zhe-yu XIE, firstName=Zhe-yu, middleName=null, lastName=XIE, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=2, address=2. Shaoxing Institute for Food and Drug Control, Shaoxing 312071, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1222469887272936436, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1222469882294297305, authorId=1222469887046444002, language=CN, stringName=谢浙裕, firstName=浙裕, middleName=null, lastName=谢, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=2, address=2.绍兴市食品药品检验研究院, 浙江 绍兴 312071, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1222469886526350256, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1222469882294297305, xref=null, ext=[AuthorCompanyExt(id=1222469886534738865, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1222469882294297305, companyId=1222469886526350256, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2. Shaoxing Institute for Food and Drug Control, Shaoxing 312071, China), AuthorCompanyExt(id=1222469886538933170, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1222469882294297305, companyId=1222469886526350256, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2.绍兴市食品药品检验研究院, 浙江 绍兴 312071)])]), Author(id=1222469887407154172, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1222469882294297305, orderNo=2, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=yjwu@zju.edu.cn, emailSecond=null, emailThird=null, correspondingAuthor=1, authorType=1, ext={EN=AuthorExt(id=1222469887562342408, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1222469882294297305, authorId=1222469887407154172, language=EN, stringName=Yong-jiang WU, firstName=Yong-jiang, middleName=null, lastName=WU, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, *, address=1. College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1222469887671394322, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1222469882294297305, authorId=1222469887407154172, language=CN, stringName=吴永江, firstName=永江, middleName=null, lastName=吴, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, *, address=1.浙江大学药学院, 浙江 杭州 310058, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1222469886396326820, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1222469882294297305, xref=null, ext=[AuthorCompanyExt(id=1222469886400521125, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1222469882294297305, companyId=1222469886396326820, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1. College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China), AuthorCompanyExt(id=1222469886408909734, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1222469882294297305, companyId=1222469886396326820, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1.浙江大学药学院, 浙江 杭州 310058)])])], keywords=[Keyword(id=1222469887776251932, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1222469882294297305, language=EN, orderNo=1, keyword=Leonurus japonicus), Keyword(id=1222469887889498151, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1222469882294297305, language=EN, orderNo=2, keyword=fingerprint), Keyword(id=1222469887994355764, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1222469882294297305, language=EN, orderNo=3, keyword=multi-component determination), Keyword(id=1222469888111796284, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1222469882294297305, language=CN, orderNo=1, keyword=益母草), Keyword(id=1222469888220848200, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1222469882294297305, language=CN, orderNo=2, keyword=指纹图谱), Keyword(id=1222469888321511506, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1222469882294297305, language=CN, orderNo=3, keyword=多成分含量测定)], refs=[Reference(id=1222469890406080749, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1222469882294297305, doi=null, pmid=null, pmcid=null, year=2000, volume=null, issue=null, pageStart=96, pageEnd=null, url=null, language=null, rfNumber=[1], rfOrder=0, authorNames=Gao XM, journalName=Traditional Chinese Medicine (中药学), refType=null, unstructuredReference= Gao XM . Traditional Chinese Medicine (中药学)[M]. Beijing, People's Medical Publishing House, 2000, 96., articleTitle=null, refAbstract=null), Reference(id=1222469890515132657, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1222469882294297305, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[2], rfOrder=1, authorNames=null, journalName=null, refType=null, unstructuredReference=Chinese Pharmacopoeia Committee. Pharmacopoeia of the People's Republic of China (Part 1) (中华人民共和国药典, 一部)[S]. Beijing: China Medical Science Press, 2015: 290-291., articleTitle=null, refAbstract=null), Reference(id=1222469890607407350, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1222469882294297305, doi=null, pmid=null, pmcid=null, year=2003, volume=13, issue=null, pageStart=349, pageEnd=350, url=http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=zgywhxzz200306010, language=null, rfNumber=[3], rfOrder=2, authorNames=Cong Y, Wang JH, Guo HR, journalName=Chin J Med Chem (中国药物化学杂志), refType=null, unstructuredReference= Cong Y , Wang JH , Guo HR et al . Isolation and identification of the chemical constituents of Leonurus japonicus Houtt. Ⅱ[J]. Chin J Med Chem (中国药物化学杂志), 2003, 13: 349-350., articleTitle=Isolation and identification of the chemical constituents of Leonurus japonicus Houtt. Ⅱ, refAbstract=null), Reference(id=1222469890783568126, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1222469882294297305, doi=null, pmid=null, pmcid=null, year=2013, volume=23, issue=null, pageStart=480, pageEnd=485, url=http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=zgywhxzz201306009, language=null, rfNumber=[4], rfOrder=3, authorNames=Zhang Y, Deng S, Li XX, journalName=Chin J Med Chem (中国药物化学杂志), refType=null, unstructuredReference= Zhang Y , Deng S , Li XX et al . Isolation and identification of constituents from Leonurus japonicus Ⅱ[J]. Chin J Med Chem (中国药物化学杂志), 2013, 23: 480-485., articleTitle=Isolation and identification of constituents from Leonurus japonicus Ⅱ, refAbstract=null), Reference(id=1222469890884231431, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1222469882294297305, doi=null, pmid=null, pmcid=null, year=2009, volume=34, issue=null, pageStart=1816, pageEnd=1818, url=http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=zgzyzz200914017, language=null, rfNumber=[5], rfOrder=4, authorNames=Cong Y, Guo JG, Wang TX, journalName=China J Chin Mater Med (中国中药杂志), refType=null, unstructuredReference= Cong Y , Guo JG , Wang TX et al . Chemical constituents and antitumor activity on leukemia K562 cell of Leonurus heterophyllus[J]. China J Chin Mater Med (中国中药杂志), 2009, 34: 1816-1818., articleTitle=Chemical constituents and antitumor activity on leukemia K562 cell of Leonurus heterophyllus, refAbstract=null), Reference(id=1222469890989089043, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1222469882294297305, doi=null, pmid=null, pmcid=null, year=2014, volume=33, issue=null, pageStart=362, pageEnd=365, url=http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=tjzyxyxb201406012, language=null, rfNumber=[6], rfOrder=5, authorNames=Deng S, Liu LL, Chen Y, journalName=J Tianjin Univ Tradit Chin Med (天津中医药大学学报), refType=null, unstructuredReference= Deng S , Liu LL , Chen Y et al . Study on chemical constituents of Leonurus japonicus Houtt. Ⅲ[J]. J Tianjin Univ Tradit Chin Med (天津中医药大学学报), 2014, 33: 362-365., articleTitle=Study on chemical constituents of Leonurus japonicus Houtt. Ⅲ, refAbstract=null), Reference(id=1222469891089752345, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1222469882294297305, doi=null, pmid=null, pmcid=null, year=2003, volume=15, issue=null, pageStart=162, pageEnd=166, url=null, language=null, rfNumber=[7], rfOrder=6, authorNames=Zhang X, Peng GP, journalName=Nat Prod Res Dev (天然产物研究与开发), refType=null, unstructuredReference= Zhang X , Peng GP . Development of chemical constituents for Leonurus[J]. Nat Prod Res Dev (天然产物研究与开发), 2003, 15: 162-166., articleTitle=Development of chemical constituents for Leonurus, refAbstract=null), Reference(id=1222469891182027039, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1222469882294297305, doi=null, pmid=null, pmcid=null, year=2015, volume=32, issue=null, pageStart=1467, pageEnd=1471, url=null, language=null, rfNumber=[8], rfOrder=7, authorNames=Zhong NY, Zhang WT, Tang DF, journalName=Chin J Mod Appl Pharm (中国现代应用药学), refType=null, unstructuredReference= Zhong NY , Zhang WT , Tang DF et al . Fingerprint method and multi-component quantification analysis method study of Leonurus liquid extract[J]. Chin J Mod Appl Pharm (中国现代应用药学), 2015, 32: 1467-1471., articleTitle=Fingerprint method and multi-component quantification analysis method study of Leonurus liquid extract, refAbstract=null), Reference(id=1222469891261718823, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1222469882294297305, doi=null, pmid=null, pmcid=null, year=2007, volume=27, issue=null, pageStart=1578, pageEnd=1581, url=http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=ywfxzz200710021, language=null, rfNumber=[9], rfOrder=8, authorNames=He YL, Jiang WD, He SF, journalName=Chin J Med Chem (药物分析杂志), refType=null, unstructuredReference= He YL , Jiang WD , He SF et al . HPLC fingerprint of Yimucao injection[J]. Chin J Med Chem (药物分析杂志), 2007, 27: 1578-1581., articleTitle=HPLC fingerprint of Yimucao injection, refAbstract=null), Reference(id=1222469891383353645, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1222469882294297305, doi=null, pmid=null, pmcid=null, year=2007, volume=38, issue=null, pageStart=1171, pageEnd=1174, url=http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=zcy200708017, language=null, rfNumber=[10], rfOrder=9, authorNames=Miao WJ, Yang L, Wu DR, journalName=Chin Tradit Herb Drugs (中草药), refType=null, unstructuredReference= Miao WJ , Yang L , Wu DR et al . Fingerprints of Leonurus heterophyllus injection with capillary electrophoresis[J]. Chin Tradit Herb Drugs (中草药), 2007, 38: 1171-1174., articleTitle=Fingerprints of Leonurus heterophyllus injection with capillary electrophoresis, refAbstract=null), Reference(id=1222469891479822645, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1222469882294297305, doi=null, pmid=null, pmcid=null, year=2016, volume=36, issue=null, pageStart=1219, pageEnd=1224, url=http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=ywfxzz201607012, language=null, rfNumber=[11], rfOrder=10, authorNames=Yang C, Bao XH, Geng Z, journalName=Chin J Med Chem (药物分析杂志), refType=null, unstructuredReference= Yang C , Bao XH , Geng Z et al . Fingerprint analysis of Yimucao injection[J]. Chin J Med Chem (药物分析杂志), 2016, 36: 1219-1224., articleTitle=Fingerprint analysis of Yimucao injection, refAbstract=null), Reference(id=1222469891605651768, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1222469882294297305, doi=null, pmid=null, pmcid=null, year=2017, volume=42, issue=null, pageStart=3523, pageEnd=3529, url=http://d.old.wanfangdata.com.cn/Periodical/zgzyzz201718014, language=null, rfNumber=[12], rfOrder=11, authorNames=Deng Z, Zhang F, Zhang J, journalName=China J Chin Mater Med (中国中药杂志), refType=null, unstructuredReference= Deng Z , Zhang F , Zhang J et al . Standard decoction of Leonuri Herba[J]. China J Chin Mater Med (中国中药杂志), 2017, 42: 3523-3529., articleTitle=Standard decoction of Leonuri Herba, refAbstract=null)], funds=[Fund(id=1222469890167005404, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1222469882294297305, awardId=2060302-1508-04, language=CN, fundingSource=名贵中药资源可持续利用能力建设项目(2060302-1508-04), fundOrder=null, country=null), Fund(id=1222469890263474404, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1222469882294297305, awardId=2018ZX09201-010, language=CN, fundingSource=国家科技重大专项“重大新药创制”项目(2018ZX09201-010), fundOrder=null, country=null)], companyList=[AuthorCompany(id=1222469886396326820, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1222469882294297305, xref=null, ext=[AuthorCompanyExt(id=1222469886400521125, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1222469882294297305, companyId=1222469886396326820, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1. College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China), AuthorCompanyExt(id=1222469886408909734, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1222469882294297305, companyId=1222469886396326820, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1.浙江大学药学院, 浙江 杭州 310058)]), AuthorCompany(id=1222469886526350256, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1222469882294297305, xref=null, ext=[AuthorCompanyExt(id=1222469886534738865, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1222469882294297305, companyId=1222469886526350256, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2. Shaoxing Institute for Food and Drug Control, Shaoxing 312071, China), AuthorCompanyExt(id=1222469886538933170, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1222469882294297305, companyId=1222469886526350256, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2.绍兴市食品药品检验研究院, 浙江 绍兴 312071)])], figs=[ArticleFig(id=1222469888535421027, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1222469882294297305, language=EN, label=null, caption=null, figureFileSmall=PXhULoohsLo4fP2UMY2ACQ==, figureFileBig=YA8bcNVw//b2Vsgh7SOctA==, tableContent=null), ArticleFig(id=1222469888627695727, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1222469882294297305, language=CN, label=Figure 1, caption= HPLC chromatograms of mixed standard solution (A) and sample solution (B). 2: Syringic acid; 6: Leonurine hydrochloride; 8: Rutin; 9: Hyperoside; 10: Isoquercitrin; 1, 3, 4, 5, 7, 11, 12: Other characteristic peak , figureFileSmall=PXhULoohsLo4fP2UMY2ACQ==, figureFileBig=YA8bcNVw//b2Vsgh7SOctA==, tableContent=null), ArticleFig(id=1222469888879353982, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1222469882294297305, language=EN, label=null, caption=null, figureFileSmall=25PhlClcGfzGfaaVURhdTg==, figureFileBig=YmaHYEUduz3cJJ4BRq5Erg==, tableContent=null), ArticleFig(id=1222469888971628678, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1222469882294297305, language=CN, label=Figure 2, caption= Fingerprints of 25 batches of <i>Leonurus japonicus</i> , figureFileSmall=25PhlClcGfzGfaaVURhdTg==, figureFileBig=YmaHYEUduz3cJJ4BRq5Erg==, tableContent=null), ArticleFig(id=1222469889063903374, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1222469882294297305, language=EN, label=null, caption=null, figureFileSmall=null, figureFileBig=null, tableContent=
Sample
No.		 Medicine site Origin Collection date
Y01 Aboveground part Sichuan Beichuan 2012.7.24
Y02 Aboveground part Sichuan Jiangyou 2012.6.13
Y03 Aboveground part Hunan Fenghuang 2012.8.5
Y04 Aboveground part Shanxi Huguan 2013.10.15
Y05 Aboveground part Yunnan Yuxi 2012.1.11
Y06 Aboveground part Gansu Weiyuan 2012.10.27
Y07 Aboveground part Gansu Weiyuan 2012.10.25
Y08 Aboveground part Gansu Weiyuan 2012.10.26
Y09 Aboveground part Henan Jiyuan 2014.5.16
Y10 Whole grass Anhui Hexian 2012.6.13
Y11 Aboveground part Anhui Hanshan 2012.6.5
Y12 Whole grass Anhui Qianshan 2012.9.22
Y13 Aboveground part Hunan Zhuzhou 2012.5.15
Y14 Aboveground part Shanxi Yangcheng 2013.9.7
Y15 Aboveground part Shanxi Changzi 2013.8.9
Y16 Aboveground part Shanxi Lingchuan 2013.7.10
Y17 Aboveground part Shanxi Hongdong 2013.8.13
Y18 Aboveground part Shanxi Yongji 2013.6.6
Y19 Aboveground part Shanxi Jiaocheng 2013.6.27
Y20 Aboveground part Shanxi Qingxu 2013.10.10
Y21 Aboveground part Shanxi Qingxu 2013.9.28
Y22 Aboveground part Shanxi Xiangfen 2013.4.16
Y23 Aboveground part Shanxi Zijiayu 2013.8.21
Y24 Aboveground part Shanxi Qingyuan 2013.9.7
Y25 Aboveground part Shanxi Wanrong 2013.9.10
), ArticleFig(id=1222469889147789462, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1222469882294297305, language=CN, label=Table 1, caption=

Sample information

, figureFileSmall=null, figureFileBig=null, tableContent=
Sample
No.		 Medicine site Origin Collection date
Y01 Aboveground part Sichuan Beichuan 2012.7.24
Y02 Aboveground part Sichuan Jiangyou 2012.6.13
Y03 Aboveground part Hunan Fenghuang 2012.8.5
Y04 Aboveground part Shanxi Huguan 2013.10.15
Y05 Aboveground part Yunnan Yuxi 2012.1.11
Y06 Aboveground part Gansu Weiyuan 2012.10.27
Y07 Aboveground part Gansu Weiyuan 2012.10.25
Y08 Aboveground part Gansu Weiyuan 2012.10.26
Y09 Aboveground part Henan Jiyuan 2014.5.16
Y10 Whole grass Anhui Hexian 2012.6.13
Y11 Aboveground part Anhui Hanshan 2012.6.5
Y12 Whole grass Anhui Qianshan 2012.9.22
Y13 Aboveground part Hunan Zhuzhou 2012.5.15
Y14 Aboveground part Shanxi Yangcheng 2013.9.7
Y15 Aboveground part Shanxi Changzi 2013.8.9
Y16 Aboveground part Shanxi Lingchuan 2013.7.10
Y17 Aboveground part Shanxi Hongdong 2013.8.13
Y18 Aboveground part Shanxi Yongji 2013.6.6
Y19 Aboveground part Shanxi Jiaocheng 2013.6.27
Y20 Aboveground part Shanxi Qingxu 2013.10.10
Y21 Aboveground part Shanxi Qingxu 2013.9.28
Y22 Aboveground part Shanxi Xiangfen 2013.4.16
Y23 Aboveground part Shanxi Zijiayu 2013.8.21
Y24 Aboveground part Shanxi Qingyuan 2013.9.7
Y25 Aboveground part Shanxi Wanrong 2013.9.10
), ArticleFig(id=1222469889235869850, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1222469882294297305, language=EN, label=null, caption=null, figureFileSmall=null, figureFileBig=null, tableContent=
Sample No. Similarity
Y01 0.921
Y02 0.948
Y03 0.933
Y04 0.971
Y05 0.900
Y06 0.967
Y07 0.985
Y08 0.976
Y09 0.972
Y10 0.951
Y11 0.958
Y12 0.962
Y13 0.942
Y14 0.972
Y15 0.975
Y16 0.955
Y17 0.978
Y18 0.912
Y19 0.970
Y20 0.912
Y21 0.948
Y22 0.973
Y23 0.983
Y24 0.924
Y25 0.956
R 1.000
), ArticleFig(id=1222469889453973668, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1222469882294297305, language=CN, label=Table 2, caption=

Fingerprint similarity of Leonurus japonicus from different origins

, figureFileSmall=null, figureFileBig=null, tableContent=
Sample No. Similarity
Y01 0.921
Y02 0.948
Y03 0.933
Y04 0.971
Y05 0.900
Y06 0.967
Y07 0.985
Y08 0.976
Y09 0.972
Y10 0.951
Y11 0.958
Y12 0.962
Y13 0.942
Y14 0.972
Y15 0.975
Y16 0.955
Y17 0.978
Y18 0.912
Y19 0.970
Y20 0.912
Y21 0.948
Y22 0.973
Y23 0.983
Y24 0.924
Y25 0.956
R 1.000
), ArticleFig(id=1222469889592385711, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1222469882294297305, language=EN, label=null, caption=null, figureFileSmall=null, figureFileBig=null, tableContent=
Analyte Origin
/mg		 Added
/mg		 Found
/mg		 Recovery
/%		 Mean
recovery
/%		 RSD
/%
Syringic acid 0.041 20 0.035 90 0.076 78 99.11 99.0 1.1
0.041 20 0.035 90 0.077 62 101.4
0.041 20 0.035 90 0.076 66 98.77
0.041 20 0.044 88 0.085 83 99.44
0.041 20 0.044 88 0.085 30 98.26
0.041 20 0.044 88 0.085 38 98.44
0.041 20 0.053 86 0.094 37 98.72
0.041 20 0.053 86 0.093 85 97.75
0.041 20 0.053 86 0.094 37 98.72
Leonurine
hydrochloride		 2.121 1.677 3.725 95.65 97.6 1.8
2.121 1.677 3.731 96.00
2.121 1.677 3.777 98.75
2.121 2.096 4.232 100.7
2.121 2.096 4.137 96.18
2.121 2.096 4.208 99.57
2.121 2.515 4.539 96.14
2.121 2.515 4.589 98.13
2.121 2.515 4.558 96.90
Rutin 1.418 1.093 2.478 96.98 97.4 1.4
1.418 1.093 2.501 99.09
1.418 1.093 2.457 95.06
1.418 1.367 2.774 99.20
1.418 1.367 2.767 98.68
1.418 1.367 2.754 97.73
1.418 1.640 3.017 97.50
1.418 1.640 3.009 97.01
1.418 1.640 2.988 95.73
Hyperoside 0.330 8 0.267 4 0.591 8 97.61 96.9 1.5
0.330 8 0.267 4 0.594 7 98.69
0.330 8 0.267 4 0.586 4 95.59
0.330 8 0.334 2 0.663 3 99.49
0.330 8 0.334 2 0.651 1 95.84
0.330 8 0.334 2 0.652 9 96.38
0.330 8 0.401 1 0.720 5 97.16
0.330 8 0.401 1 0.711 7 94.96
0.330 8 0.401 1 0.716 7 96.21
Isoquercitrin 1.238 0.893 2.101 96.64 98.5 1.3
1.238 0.893 2.113 97.98
1.238 0.893 2.125 99.33
1.238 1.116 2.337 98.48
1.238 1.116 2.362 100.7
1.238 1.116 2.325 97.40
1.238 1.339 2.545 97.61
1.238 1.339 2.562 98.88
1.238 1.339 2.573 99.70
), ArticleFig(id=1222469889714020537, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1222469882294297305, language=CN, label=Table 3, caption=

Recovery of 5 analytes in Leonurus japonicus

, figureFileSmall=null, figureFileBig=null, tableContent=
Analyte Origin
/mg		 Added
/mg		 Found
/mg		 Recovery
/%		 Mean
recovery
/%		 RSD
/%
Syringic acid 0.041 20 0.035 90 0.076 78 99.11 99.0 1.1
0.041 20 0.035 90 0.077 62 101.4
0.041 20 0.035 90 0.076 66 98.77
0.041 20 0.044 88 0.085 83 99.44
0.041 20 0.044 88 0.085 30 98.26
0.041 20 0.044 88 0.085 38 98.44
0.041 20 0.053 86 0.094 37 98.72
0.041 20 0.053 86 0.093 85 97.75
0.041 20 0.053 86 0.094 37 98.72
Leonurine
hydrochloride		 2.121 1.677 3.725 95.65 97.6 1.8
2.121 1.677 3.731 96.00
2.121 1.677 3.777 98.75
2.121 2.096 4.232 100.7
2.121 2.096 4.137 96.18
2.121 2.096 4.208 99.57
2.121 2.515 4.539 96.14
2.121 2.515 4.589 98.13
2.121 2.515 4.558 96.90
Rutin 1.418 1.093 2.478 96.98 97.4 1.4
1.418 1.093 2.501 99.09
1.418 1.093 2.457 95.06
1.418 1.367 2.774 99.20
1.418 1.367 2.767 98.68
1.418 1.367 2.754 97.73
1.418 1.640 3.017 97.50
1.418 1.640 3.009 97.01
1.418 1.640 2.988 95.73
Hyperoside 0.330 8 0.267 4 0.591 8 97.61 96.9 1.5
0.330 8 0.267 4 0.594 7 98.69
0.330 8 0.267 4 0.586 4 95.59
0.330 8 0.334 2 0.663 3 99.49
0.330 8 0.334 2 0.651 1 95.84
0.330 8 0.334 2 0.652 9 96.38
0.330 8 0.401 1 0.720 5 97.16
0.330 8 0.401 1 0.711 7 94.96
0.330 8 0.401 1 0.716 7 96.21
Isoquercitrin 1.238 0.893 2.101 96.64 98.5 1.3
1.238 0.893 2.113 97.98
1.238 0.893 2.125 99.33
1.238 1.116 2.337 98.48
1.238 1.116 2.362 100.7
1.238 1.116 2.325 97.40
1.238 1.339 2.545 97.61
1.238 1.339 2.562 98.88
1.238 1.339 2.573 99.70
), ArticleFig(id=1222469889865015496, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1222469882294297305, language=EN, label=null, caption=null, figureFileSmall=null, figureFileBig=null, tableContent=
Sample
No.		 Syringic acid
/μg·g -1		 Leonurine
hydrochloride
/mg·g -1		 Rutin
/mg·g -1		 Hyperoside
/mg·g -1		 Isoquercitrin
/mg·g -1
Y01 28.88 1.47 0.69 0.07 0.43
Y02 43.89 2.69 0.79 0.07 0.49
Y03 37.59 1.21 1.00 0.13 0.64
Y04 21.72 2.59 1.09 0.22 0.78
Y05 44.98 5.83 0.98 0.51 1.20
Y06 33.38 2.03 1.46 0.38 1.46
Y07 41.20 2.12 1.42 0.33 1.24
Y08 32.57 2.04 1.34 0.24 0.94
Y09 27.20 5.28 1.12 0.37 1.09
Y10 84.90 3.15 1.14 0.26 0.88
Y11 158.64 1.14 4.75 0.68 2.94
Y12 74.08 2.92 1.31 0.32 1.03
Y13 69.19 1.75 2.57 0.59 2.01
Y14 79.43 7.08 3.18 0.48 1.76
Y15 32.22 4.59 1.19 0.38 1.33
Y16 46.89 7.44 1.48 0.46 1.43
Y17 44.31 6.01 2.43 0.35 1.10
Y18 44.35 5.82 1.01 0.32 1.00
Y19 23.15 2.37 1.27 0.16 0.81
Y20 124.76 11.78 4.70 0.38 1.40
Y21 32.50 3.50 4.06 0.54 3.14
Y22 32.35 5.33 1.42 0.34 1.47
Y23 36.08 3.39 1.27 0.30 1.13
Y24 33.70 4.64 1.54 0.24 1.38
Y25 35.17 7.76 2.31 0.50 1.85
), ArticleFig(id=1222469889978261712, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1222469882294297305, language=CN, label=Table 4, caption=

Determination results of 25 samples

, figureFileSmall=null, figureFileBig=null, tableContent=
Sample
No.		 Syringic acid
/μg·g -1		 Leonurine
hydrochloride
/mg·g -1		 Rutin
/mg·g -1		 Hyperoside
/mg·g -1		 Isoquercitrin
/mg·g -1
Y01 28.88 1.47 0.69 0.07 0.43
Y02 43.89 2.69 0.79 0.07 0.49
Y03 37.59 1.21 1.00 0.13 0.64
Y04 21.72 2.59 1.09 0.22 0.78
Y05 44.98 5.83 0.98 0.51 1.20
Y06 33.38 2.03 1.46 0.38 1.46
Y07 41.20 2.12 1.42 0.33 1.24
Y08 32.57 2.04 1.34 0.24 0.94
Y09 27.20 5.28 1.12 0.37 1.09
Y10 84.90 3.15 1.14 0.26 0.88
Y11 158.64 1.14 4.75 0.68 2.94
Y12 74.08 2.92 1.31 0.32 1.03
Y13 69.19 1.75 2.57 0.59 2.01
Y14 79.43 7.08 3.18 0.48 1.76
Y15 32.22 4.59 1.19 0.38 1.33
Y16 46.89 7.44 1.48 0.46 1.43
Y17 44.31 6.01 2.43 0.35 1.10
Y18 44.35 5.82 1.01 0.32 1.00
Y19 23.15 2.37 1.27 0.16 0.81
Y20 124.76 11.78 4.70 0.38 1.40
Y21 32.50 3.50 4.06 0.54 3.14
Y22 32.35 5.33 1.42 0.34 1.47
Y23 36.08 3.39 1.27 0.30 1.13
Y24 33.70 4.64 1.54 0.24 1.38
Y25 35.17 7.76 2.31 0.50 1.85
)], attaches=null, journal=Journal(id=1189982048455397383, delFlag=0, nameCn=药学学报, nameEn=Acta Pharmaceutica Sinica, nameHistory1=null, nameHistory2=null, issn=0513-4870, eissn=null, cn=11-2163/R, coden=null, periodic=0, language=CN, oaType=null, ccby=null, superviseOffice=null, ownerOffice=null, pubOffice=null, editorOffice=null, officeType=null, aims=null, clcCode=null, officeProv=null, officeCity=null, officeAddr=null, officeZip=null, officeEmail=null, officePhone=null, editDirector=null, officeDirector=null, officeDirectorPhone=null, officeStaffNum=null, officeEmpNum=null, coverPicUrl=BTxjudbJDVO4PqdBR6On6Q==, journalPrice=null, startedYear=null, abbrevIsoEn=null, journalRemark=null, publicationField=null, createdTime=1761643429151, updatedTime=1788948913902, createdBy=18614031015, updatedBy=13041195026, firstLetterCn=Y, firstLetterEn=Y, subjectCode=Medical and Pharmaceutical Sciences, subjectName=Life Sciences, subjectCodeEn=Medical and Pharmaceutical Sciences, subjectNameEn=null, picCn=BTxjudbJDVO4PqdBR6On6Q==, picEn=c4l1ckL55nWbhl1KrFdWIA==, jcr=null, cjcr=null, exts=[JournalExt(id=1304509553505227679, language=CN, name=药学学报, nameHistory1=null, nameHistory2=null, managedBy=中国科学技术协会, sponsoredBy=中国药学会、中国医学科学院药物研究所, publishedBy=《药学学报》编辑委员会编辑出版, editorOffice=, officeProv=null, officeCity=null, officeAddr=, officeZip=, editDirector=, officeDirector=null, officePhone=null, coverPicUrl=null, journalRemark=, submitArticleUrl=null, websiteUrl=, createdTime=1788948914171, updatedTime=1788948914171, createdBy=13041195026, updatedBy=13041195026, submissionGuidelinesUrl=, submissionAuthorUrl=https://www.yxxb.com.cn/journalx_yxxb/authorLogOn.action, submissionEditorUrl=https://www.yxxb.com.cn/journalx_yxxb/editorLogOn.action, submissionReviewUrl=https://www.yxxb.com.cn/journalx_yxxb/expertLogOn.action, submissionCeEditorUrl=, submissionAeEditorUrl=, option={"copyright":""}), JournalExt(id=1304509553559753632, language=EN, name=Acta Pharmaceutica Sinica, nameHistory1=null, nameHistory2=null, managedBy=, sponsoredBy=, publishedBy=, editorOffice=, officeProv=null, officeCity=null, officeAddr=, officeZip=, editDirector=, officeDirector=null, officePhone=null, coverPicUrl=null, journalRemark=, submitArticleUrl=null, websiteUrl=, createdTime=1788948914184, updatedTime=1788948914184, createdBy=13041195026, updatedBy=13041195026, submissionGuidelinesUrl=, submissionAuthorUrl=https://www.yxxb.com.cn/journalx_yxxb/authorLogOn.action, submissionEditorUrl=https://www.yxxb.com.cn/journalx_yxxb/editorLogOn.action, submissionReviewUrl=https://www.yxxb.com.cn/journalx_yxxb/expertLogOn.action, submissionCeEditorUrl=, submissionAeEditorUrl=, option={"copyright":""})], databaseList=null, tenantJournalId=1189982191388893191, websiteList=[Website(id=1189982271588340489, webName=null, webTitle=null, webDomain=null, webCopyrigh=null, webIpcNo=null, seoTitle=null, seoKeywords=null, seoDescription=null, tenantJournalId=null, journalId=1189982191388893191, journalNameCn=null, journalNameEn=null, grayFlag=null, tenantId=1146029695717560320, platformId=null, journalGroupId=null, journalGroupNameCn=null, journalGroupNameEn=null, type=1, domain=https://castjournals.cast.org.cn/joweb/yxxb/CN, language=CN, createTime=1761643482348, createBy=18614031015, updateTime=1761643498101, updateBy=18614031015, name=药学学报-中文, tplId=1146099689490845704, title=药学学报, delFlag=0, indexPage=/home, props=[WebsiteProps(id=1189982873114448678, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1189982271588340489, code=articleTextType, value=kx, createTime=1761643625763, updateTime=1761643625763, creator=18614031015, updator=18614031015), WebsiteProps(id=1189982873093477155, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1189982271588340489, code=banner, value=null, createTime=1761643625758, updateTime=1761643625758, creator=18614031015, updator=18614031015), WebsiteProps(id=1189982873135420201, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1189982271588340489, code=grayFlag, value=0, createTime=1761643625768, updateTime=1761643625768, creator=18614031015, updator=18614031015), WebsiteProps(id=1189982873085088546, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1189982271588340489, code=logo, value=https://castjournals.cast.org.cn/joweb/yxxb/CN/file/pic?fileId=w+t2v8bJnX5lh3+hRRJcDA==, createTime=1761643625756, updateTime=1761643625756, creator=18614031015, updator=18614031015), WebsiteProps(id=1189982873152197419, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1189982271588340489, code=minRunFlag, value=0, createTime=1761643625772, updateTime=1761643625772, creator=18614031015, updator=18614031015), WebsiteProps(id=1189982873110254373, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1189982271588340489, code=picServerUrl, value=https://castjournals.cast.org.cn/joweb/yxxb/CN/file/pic, createTime=1761643625762, updateTime=1761643625762, creator=18614031015, updator=18614031015), WebsiteProps(id=1189982873143808810, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1189982271588340489, code=silenceFlag, value=0, createTime=1761643625770, updateTime=1761643625770, creator=18614031015, updator=18614031015), WebsiteProps(id=1189982873101865764, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1189982271588340489, code=staticResourcePath, value=https://castjournals.cast.org.cn/joweb/cast_kjdb_cn_619/, createTime=1761643625760, updateTime=1761643625760, creator=18614031015, updator=18614031015), WebsiteProps(id=1189982873122837287, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1189982271588340489, code=themeColor, value=null, createTime=1761643625765, updateTime=1761643625765, creator=18614031015, updator=18614031015), WebsiteProps(id=1189982873127031592, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1189982271588340489, code=themeStyle, value=null, createTime=1761643625766, updateTime=1761643625766, creator=18614031015, updator=18614031015)]), Website(id=1189982271655449355, webName=null, webTitle=null, webDomain=null, webCopyrigh=null, webIpcNo=null, seoTitle=null, seoKeywords=null, seoDescription=null, tenantJournalId=null, journalId=1189982191388893191, journalNameCn=null, journalNameEn=null, grayFlag=null, tenantId=1146029695717560320, platformId=null, journalGroupId=null, journalGroupNameCn=null, journalGroupNameEn=null, type=1, domain=https://castjournals.cast.org.cn/joweb/yxxb/EN, language=EN, createTime=1761643482364, createBy=18614031015, updateTime=1761643514085, updateBy=18614031015, name=药学学报-英文, tplId=1146101810881728533, title=Acta Pharmaceutica Sinica, delFlag=0, indexPage=/home, props=[WebsiteProps(id=1189982903015633534, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1189982271655449355, code=articleTextType, value=kx, createTime=1761643632892, updateTime=1761643632892, creator=18614031015, updator=18614031015), WebsiteProps(id=1189982902990467707, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1189982271655449355, code=banner, value=null, createTime=1761643632886, updateTime=1761643632886, creator=18614031015, updator=18614031015), WebsiteProps(id=1189982903036605057, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1189982271655449355, code=grayFlag, value=0, createTime=1761643632897, updateTime=1761643632897, creator=18614031015, updator=18614031015), WebsiteProps(id=1189982902982079098, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1189982271655449355, code=logo, value=https://castjournals.cast.org.cn/joweb/yxxb/EN/file/pic?fileId=w+t2v8bJnX5lh3+hRRJcDA==, createTime=1761643632884, updateTime=1761643632884, creator=18614031015, updator=18614031015), WebsiteProps(id=1189982903053382275, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1189982271655449355, code=minRunFlag, value=0, createTime=1761643632901, updateTime=1761643632901, creator=18614031015, updator=18614031015), WebsiteProps(id=1189982903007244925, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1189982271655449355, code=picServerUrl, value=https://castjournals.cast.org.cn/joweb/yxxb/EN/file/pic, createTime=1761643632890, updateTime=1761643632890, creator=18614031015, updator=18614031015), WebsiteProps(id=1189982903044993666, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1189982271655449355, code=silenceFlag, value=0, createTime=1761643632899, updateTime=1761643632899, creator=18614031015, updator=18614031015), WebsiteProps(id=1189982902998856316, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1189982271655449355, code=staticResourcePath, value=https://castjournals.cast.org.cn/joweb/cast_kjdb_en_623/, createTime=1761643632888, updateTime=1761643632888, creator=18614031015, updator=18614031015), WebsiteProps(id=1189982903019827839, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1189982271655449355, code=themeColor, value=null, createTime=1761643632893, updateTime=1761643632893, creator=18614031015, updator=18614031015), WebsiteProps(id=1189982903028216448, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1189982271655449355, code=themeStyle, value=null, createTime=1761643632895, updateTime=1761643632895, creator=18614031015, updator=18614031015)])], journalTitle=药学学报, weixinUrl=null, journalUrl=https://www.yxxb.com.cn/aps, iacademicId=null, status=1, seqNo=null, journalTitleEn=Acta Pharmaceutica Sinica, journalPhotoCn=BTxjudbJDVO4PqdBR6On6Q==, journalPhotoEn=c4l1ckL55nWbhl1KrFdWIA==, journalFirstLetter=Y, journalRecommend=null, journalNew=null, journalCollection=null, jcrJf=null, cjcrJf=null, jcrJfStr=null, cjcrJfStr=null, submissionFirstDecision=null, sciSubjectClassification=null, casSubjectClassification=null, citeScore=null, totalCitationFrequency=null, icpCode=null, psCode=null, advertisingLicenseCode=null, copyrightInformation=null, country=null, option=, provinceCode=null, provinceName=null, collectFlag=false, interPubPlatform=, interPubPlatformUrl=null), detailUrlCn=https://castjournals.cast.org.cn/joweb/yxxb/CN/10.16438/j.0513-4870.2019-0407, detailUrlEn=https://castjournals.cast.org.cn/joweb/yxxb/EN/10.16438/j.0513-4870.2019-0407, pdfUrlCn=https://castjournals.cast.org.cn/joweb/yxxb/CN/PDF/10.16438/j.0513-4870.2019-0407, pdfUrlEn=https://castjournals.cast.org.cn/joweb/yxxb/EN/PDF/10.16438/j.0513-4870.2019-0407, aliStartDate=null, aliEndDate=null, collectionFlag=false, citedCount=null, citedUrl=null, previewStatus=0, delFlag=0, hasFullText=1, orderTime=1573488000000, fullTextJson=null, articleText=null, reference=null)
收藏切换
益母草HPLC指纹图谱及多成分含量测定方法研究
收藏切换
PDF下载
孙晨 1, 2 , 谢浙裕 2 , 吴永江 1, *
药学学报 | 研究论文 2019,54(11): 2059-2063
收起
收藏切换
药学学报 |研究论文 2019 , 54 (11) : 2059 -2063
益母草HPLC指纹图谱及多成分含量测定方法研究
全屏
[Author(id=1222469886652179390, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1222469882294297305, orderNo=0, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1222469886790591433, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1222469882294297305, authorId=1222469886652179390, language=EN, stringName=Chen SUN, firstName=Chen, middleName=null, lastName=SUN, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, 2, address=1. College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China
2. Shaoxing Institute for Food and Drug Control, Shaoxing 312071, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1222469886920614867, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1222469882294297305, authorId=1222469886652179390, language=CN, stringName=孙晨, firstName=晨, middleName=null, lastName=孙, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, 2, address=1.浙江大学药学院, 浙江 杭州 310058
2.绍兴市食品药品检验研究院, 浙江 绍兴 312071, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1222469886396326820, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1222469882294297305, xref=null, ext=[AuthorCompanyExt(id=1222469886400521125, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1222469882294297305, companyId=1222469886396326820, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1. College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China), AuthorCompanyExt(id=1222469886408909734, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1222469882294297305, companyId=1222469886396326820, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1.浙江大学药学院, 浙江 杭州 310058)]), AuthorCompany(id=1222469886526350256, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1222469882294297305, xref=null, ext=[AuthorCompanyExt(id=1222469886534738865, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1222469882294297305, companyId=1222469886526350256, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2. Shaoxing Institute for Food and Drug Control, Shaoxing 312071, China), AuthorCompanyExt(id=1222469886538933170, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1222469882294297305, companyId=1222469886526350256, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2.绍兴市食品药品检验研究院, 浙江 绍兴 312071)])]), Author(id=1222469887046444002, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1222469882294297305, orderNo=1, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1222469887168078828, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1222469882294297305, authorId=1222469887046444002, language=EN, stringName=Zhe-yu XIE, firstName=Zhe-yu, middleName=null, lastName=XIE, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=2, address=2. Shaoxing Institute for Food and Drug Control, Shaoxing 312071, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1222469887272936436, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1222469882294297305, authorId=1222469887046444002, language=CN, stringName=谢浙裕, firstName=浙裕, middleName=null, lastName=谢, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=2, address=2.绍兴市食品药品检验研究院, 浙江 绍兴 312071, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1222469886526350256, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1222469882294297305, xref=null, ext=[AuthorCompanyExt(id=1222469886534738865, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1222469882294297305, companyId=1222469886526350256, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2. Shaoxing Institute for Food and Drug Control, Shaoxing 312071, China), AuthorCompanyExt(id=1222469886538933170, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1222469882294297305, companyId=1222469886526350256, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2.绍兴市食品药品检验研究院, 浙江 绍兴 312071)])]), Author(id=1222469887407154172, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1222469882294297305, orderNo=2, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=yjwu@zju.edu.cn, emailSecond=null, emailThird=null, correspondingAuthor=1, authorType=1, ext={EN=AuthorExt(id=1222469887562342408, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1222469882294297305, authorId=1222469887407154172, language=EN, stringName=Yong-jiang WU, firstName=Yong-jiang, middleName=null, lastName=WU, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, *, address=1. College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1222469887671394322, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1222469882294297305, authorId=1222469887407154172, language=CN, stringName=吴永江, firstName=永江, middleName=null, lastName=吴, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, *, address=1.浙江大学药学院, 浙江 杭州 310058, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1222469886396326820, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1222469882294297305, xref=null, ext=[AuthorCompanyExt(id=1222469886400521125, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1222469882294297305, companyId=1222469886396326820, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1. College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China), AuthorCompanyExt(id=1222469886408909734, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1222469882294297305, companyId=1222469886396326820, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1.浙江大学药学院, 浙江 杭州 310058)])])]
孙晨1, 2, 谢浙裕2, 吴永江1, *
作者信息
  • 1.浙江大学药学院, 浙江 杭州 310058
  • 2.绍兴市食品药品检验研究院, 浙江 绍兴 312071
通讯作者:
*吴永江 ,Tel: 86-571-88208455, E-mail:
Fingerprint analysis and multi-component determination of Leonurus japonicus by high performance liquid chromatography
Chen SUN1, 2, Zhe-yu XIE2, Yong-jiang WU1, *
Affiliations
  • 1. College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China
  • 2. Shaoxing Institute for Food and Drug Control, Shaoxing 312071, China
出版时间: 2019-11-12 doi: 10.16438/j.0513-4870.2019-0407
文章导航
收藏切换
摘要
收起

建立益母草的HPLC指纹图谱及多成分含量同时测定方法，为益母草质量综合评价和控制提供参考。益母草用70%乙醇水浴回流提取2 h，提取物采用HPLC法分析，色谱柱为Kromasil C18（250mm×4.6 mm，5 μm），流动相为乙腈-0.1%甲酸，梯度洗脱，流速为1.0 mL·min-1，柱温30℃，检测波长为280 nm。25批样品得到共有峰12个，确认了其中5个峰对应的化学成分。丁香酸、盐酸益母草碱、芦丁、金丝桃苷和异槲皮苷分别在进样量0.426 1~85.22ng（r=0.999 9）、7.948~1590 ng（r=0.999 3）、10.20~2040 ng（r=1.000 0）、2.018~403.6ng（r=0.999 9）、8.704~1741 ng（r=0.999 9）内呈良好的线性关系；平均回收率分别为99.0%、97.6%、97.4%、96.9%和98.5%，RSD分别为1.1%、1.8%、1.4%、1.5%和1.3%。该指纹图谱特征性明显，并可同时测定5个成分的含量。

益母草  /  指纹图谱  /  多成分含量测定

An HPLC fingerprint and multi-component determination method of Leonurus japonicus was established for comprehensive evaluation and quality control of Leonurus japonicus. The sample was incubated in 70% ethanol in a water bath for 2 h, and the extract was analyzed by HPLC using Kromasil C18 column (250 mm×4.6 mm, 5 μm). The mobile phase consisted of acetonitrile-0.1% formic acid with gradient elution. The flow rate was 1.0 mL·min-1. The temperature of column was 30℃. The detection wavelength was 280 nm. HPLC fingerprint of characteristic components of Leonurus japonicus was established. There were 12 common peaks among 25 batches of samples, and 5 of them were identified and determined. Syringic acid, leonurine hydrochloride, rutin, hyperoside or isoquercitrin showed a good linearity in the ranges of 0.426 1-85.22 ng (r=0.999 9), 7.948-1 590 ng (r=0.999 3), 10.20-2 040 ng (r=1.000 0), 2.018-403.6 ng (r=0.999 9), or 8.704-1 741 ng (r=0.999 9), respectively. The average recoveries were 99.0%, 97.6%, 97.4%, 96.9% and 98.5% with RSD of 1.1%, 1.8%, 1.4%, 1.5% and 1.3%, respectively. The HPLC characteristic fingerprint of Leonurus japonicus was specific, and this method can simultaneously determine the content of 5 components.

Leonurus japonicus  /  fingerprint  /  multi-component determination
孙晨, 谢浙裕, 吴永江. 益母草HPLC指纹图谱及多成分含量测定方法研究. 药学学报, 2019 , 54 (11) : 2059 -2063 . DOI: 10.16438/j.0513-4870.2019-0407
Chen SUN, Zhe-yu XIE, Yong-jiang WU. Fingerprint analysis and multi-component determination of Leonurus japonicus by high performance liquid chromatography[J]. Acta Pharmaceutica Sinica, 2019 , 54 (11) : 2059 -2063 . DOI: 10.16438/j.0513-4870.2019-0407
正文
收起
益母草为唇形科植物益母草(Leonurus japonicus Houtt.)的新鲜或干燥地上部分, 始载于《神农本草经》, 列为上品, 历代本草均有收载[1]。益母草味辛苦、凉, 具有活血调经、利尿消肿、清热解毒的功效[2], 临床上广泛用于月经不调、产后瘀痛、痛经闭经、恶露不尽、水肿尿少、急性肾炎水肿、心脑血管疾病、血液病等疾病的治疗。益母草成分较复杂, 目前从中先后分离得到黄酮类[3-5]、生物碱类[6, 7]等化学成分, 而现行质量标准中只对盐酸水苏碱和盐酸益母草碱进行控制, 没有关注到其他化学成分的情况, 难以从整体上把握益母草的内在品质。指纹图谱作为中药质量控制的重要手段, 已被广泛应用, 有文献报道对益母草流浸膏[8]、益母草注射液[9-11]、益母草饮片标准汤剂[12]的指纹图谱进行了研究, 但未对益母草药材进行指纹图谱研究, 也未见益母草指纹图谱与含量测定相结合的质量分析方法。本文运用高效液相色谱法, 对其指纹图谱方法进行研究, 并同时测定5个成分的含量, 建立益母草指纹图谱与含量测定相结合的质控方法, 以期为益母草质量控制提供科学依据。
材料与方法
收起
仪器   LC-20A高效液相色谱仪(日本岛津公司); XPE-205电子天平(Mettler Toledo); MILLI-Q ADVANTAGE A 10超纯水仪(MILLIPORE); TW-20恒温水浴锅(德国优莱博); 5810R台式大容量冷冻离心机(德国EPPENDORF公司)。
试药、试剂与样品   丁香酸对照品(阿拉丁, 批号22852, 纯度98%); 盐酸益母草碱对照品(中国药品生物制品检定所, 批号111823-201303, 含量95.2%); 芦丁对照品(批号100080-201408, 含量90.2%)、金丝桃苷对照品(批号111521-201507, 含量为94.3%)、异槲皮苷对照品(批号111809-201403, 含量92.9%)购自中国食品药品检定研究院; 乙腈(Merck, 色谱级); 超纯水; 其余试剂为分析纯; 25批益母草由浙江省食品药品检验研究院中药所提供, 样品信息见表 1。
对照品溶液的制备   精密称取丁香酸、盐酸益母草碱、芦丁、金丝桃苷、异槲皮苷对照品适量, 加70%乙醇制成浓度分别为4.261、79.48、102.0、20.18、87.04 μg·mL-1的混合对照品溶液。
供试品溶液的制备   精密称取益母草2.0 g置烧瓶中, 精密加入50 mL 70%乙醇, 水浴回流2 h, 放冷, 补足减失重量, 摇匀, 离心, 取上清液, 即得。
色谱条件   色谱柱: Kromasil C18 (250 mm×4.6 mm, 5 μm); 流动相:乙腈(A)-0.1%甲酸(B), 梯度洗脱程序如下: 0→60 min, 5% A→25% A; 60→60.1 min, 25% A→80% A; 60.1→70 min, 80% A→80% A; 70→70.1 min, 80% A→5% A; 70.1→80 min, 5% A→5% A。柱温: 30 ℃; 流速: 1.0 mL·min-1; 检测波长: 280 nm; 进样量: 5 μL。
指纹图谱方法学验证
重复性   取同一批益母草(Y07) 6份, 按“供试品溶液的制备”方法制得供试品溶液, 在上述色谱条件下进样分析, 记录色谱图, 测定主要共有峰的相对保留时间和相对峰面积。
精密度   取益母草(Y07), 按“供试品溶液的制备”方法制得供试品溶液, 在上述色谱条件下连续进样6次, 记录色谱图, 测定主要共有峰的相对保留时间和相对峰面积。
稳定性   取益母草(Y07), 按“供试品溶液的制备”方法制得供试品溶液, 在上述色谱条件下分别于0、4、8、12、16、20、24 h进样, 记录色谱图, 测定主要共有峰的相对保留时间和相对峰面积。
指纹图谱建立及相似度评价   取25批益母草样品, 按“供试品溶液的制备”项下方法制备供试品溶液, 在上述色谱条件下进样分析, 记录色谱图。将图谱导入国家药典委员会开发的中药色谱指纹图谱相似度评价系统2.0版中进行分析, 生成对照图谱, 计算各样品指纹图谱与对照图谱的相似度。
多指标含量测定方法学验证
根据指纹图谱研究结果, 对已知的5个成分丁香酸、盐酸益母草碱、芦丁、金丝桃苷、异槲皮苷进行定量测定方法验证。
线性关系   分别依次精密吸取混合对照品溶液0.1、0.2、0.5、1、2、5、10、20 μL注入液相色谱仪, 记录色谱图, 以峰面积(Y)对进样量(X)进行线性回归, 计算回归方程和相关系数。
重复性   按指纹图谱重复性项下方法, 记录丁香酸、盐酸益母草碱、芦丁、金丝桃苷、异槲皮苷的峰面积, 计算RSD值。
稳定性   按指纹图谱稳定性项下方法, 记录丁香酸、盐酸益母草碱、芦丁、金丝桃苷、异槲皮苷的峰面积, 计算RSD值。
回收率   精密称取已知含量的益母草(Y12) 9份, 分别按低、中、高3个梯度加入一定量的混合对照品溶液(5种成分大致为样品中含量的80%、100%、120%), 制备供试品溶液, 测定各成分含量, 计算平均回收率和RSD。
结果
收起
1 指纹图谱方法学考察
在上述色谱条件下, 各特征峰可达到较好分离, 混合对照品与供试品溶液HPLC色谱图见图 1。
1.1 重复性
12个共有峰相对保留时间的RSD均 < 0.4%, 相对峰面积均 < 1.7%, 说明本法具有较好的重复性。
1.2 精密度
12个共有峰相对保留时间的RSD均 < 0.2%, 相对峰面积均 < 0.5%, 说明该方法精密度良好。
1.3 稳定性
12个共有峰相对保留时间的RSD均 < 0.3%, 相对峰面积均 < 1.4%, 表明供试品在24 h内稳定。
2 HPLC指纹图谱建立及相似度评价
25批益母草的指纹图谱见图 2, 共有12个特征峰。通过对照品定位, 高分辨质谱确证, 指认其中2号峰为丁香酸, 6号峰为盐酸益母草碱, 8号峰为芦丁, 9号峰为金丝桃苷, 10号峰为异槲皮苷, 其中盐酸益母草碱为益母草的活性成分和指标性成分之一, 含量较高且稳定, 且保留时间处于整个图谱中间位置, 选择盐酸益母草碱作为参照峰。所收集的25批样品指纹图谱与对照图谱相比, 相似度均 > 0.90, 结果见表 2, 表明不同产地的益母草整体质量差异较小。
3 多指标含量测定方法学考察
3.1 线性关系
丁香酸、盐酸益母草碱、芦丁、金丝桃苷和异槲皮苷的线性方程分别为: y = 108 121x + 4 469.9 (r = 0.999 9); y = 64 532x + 10 051 (r = 0.999 3); y = 32 742x + 1 163.5 (r = 1.0000); y = 38 177x + 1 224.1 (r = 0.999 9); y = 42 796x + 978.01 (r = 0.999 9)。各成分进样量分别在0.426 1~85.22 ng、7.948~1 590 ng、10.20~2 040 ng、2.018~403.6 ng、8.704~1 741 ng内呈良好的线性关系。
3.2 重复性
丁香酸、盐酸益母草碱、芦丁、金丝桃苷、异槲皮苷峰面积的RSD分别为0.9%、1.1%、1.6%、1.3%和0.4% (n = 6), 表明重复性良好。
3.3 稳定性
丁香酸、盐酸益母草碱、芦丁、金丝桃苷、异槲皮苷峰面积的RSD分别为1.2%、1.5%、1.7%、1.6%和1.4%, 表明供试品溶液在24 h内稳定。
3.4 回收率
丁香酸、盐酸益母草碱、芦丁、金丝桃苷、异槲皮苷的平均回收率分别为99.0%、97.6%、97.4%、96.9%和98.5%, RSD分别为1.1%、1.8%、1.4%、1.5%和1.3%, 结果见表 3。
4 样品含量测定
取混合对照品溶液和25批益母草样品供试品溶液, 按上述色谱条件进样分析, 按外标法以峰面积计算含量, 结果见表 4。
讨论
收起
由于益母草含有的成分极性差异大, 采用等度洗脱难以使各成分得到分离, 不能使指纹图谱获得尽可能多的信息量。为了使主要成分在一张图谱上反映出来, 并能有效分离, 采用了梯度洗脱。尝试了有机相甲醇和乙腈, 乙腈分离效果较好; 水相尝试了0.1%甲酸、0.01%三氟乙酸、0.02 mol·L-1甲酸铵溶液、0.02 mol·L-1乙酸铵溶液, 0.1%甲酸和0.01%三氟乙酸效果均较为理想, 考虑到质谱不能使用三氟乙酸做流动相, 因此选择了0.1%甲酸。色谱柱尝试了Kromasil C18、Diamonsil C18、Venusil MP C18、Waters Symmetry C18、Welch Materials XB-C18、Shimadzu VP-ODS C18、Agilent SB C18等, Kromasil C18和Venusil MP C18分离效果较为理想, 最后选择了Kromasil C18。5种成分含量测定分离度均大于1.5, 理论板数均大于10 000。在样品处理中考察了水、甲醇和不同浓度乙醇等多种提取溶媒, 同时考察了不同时间的超声和回流提取, 并对5种成分进行全波长扫描筛选检测波长, 最终确定了色谱条件和样品制备方法。
本实验建立的益母草指纹图谱有12个共有峰, 所收集的25批样品指纹图谱与对照图谱相比, 相似度均 > 0.90, 表明不同产地益母草的整体质量一致性较好。在建立指纹图谱方法的基础上, 对益母草5个成分进行了含量测定, 可同时控制特征图谱中多成分的含量。对所收集的25批益母草样品进行含量测定, 发现各产地丁香酸、金丝桃苷、盐酸益母草碱、芦丁、异槲皮苷的含量差异较大, 可能与气候、土质等有关。经方法学研究表明, 本文方法重现性良好, 操作简便, 可作为益母草质量鉴定的依据。
基金
收起
  • 名贵中药资源可持续利用能力建设项目(2060302-1508-04)
  • 国家科技重大专项“重大新药创制”项目(2018ZX09201-010)
文献
收起
参考文献 引证文献
排序方式：
[1]
Gao XM . Traditional Chinese Medicine (中药学)[M]. Beijing, People's Medical Publishing House, 2000, 96.
[2]
Chinese Pharmacopoeia Committee. Pharmacopoeia of the People's Republic of China (Part 1) (中华人民共和国药典, 一部)[S]. Beijing: China Medical Science Press, 2015: 290-291.
[3]
Cong Y , Wang JH , Guo HR et al . Isolation and identification of the chemical constituents of Leonurus japonicus Houtt. Ⅱ[J]. Chin J Med Chem (中国药物化学杂志), 2003, 13: 349-350. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=zgywhxzz200306010
[4]
Zhang Y , Deng S , Li XX et al . Isolation and identification of constituents from Leonurus japonicus Ⅱ[J]. Chin J Med Chem (中国药物化学杂志), 2013, 23: 480-485. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=zgywhxzz201306009
[5]
Cong Y , Guo JG , Wang TX et al . Chemical constituents and antitumor activity on leukemia K562 cell of Leonurus heterophyllus[J]. China J Chin Mater Med (中国中药杂志), 2009, 34: 1816-1818. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=zgzyzz200914017
[6]
Deng S , Liu LL , Chen Y et al . Study on chemical constituents of Leonurus japonicus Houtt. Ⅲ[J]. J Tianjin Univ Tradit Chin Med (天津中医药大学学报), 2014, 33: 362-365. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=tjzyxyxb201406012
[7]
Zhang X , Peng GP . Development of chemical constituents for Leonurus[J]. Nat Prod Res Dev (天然产物研究与开发), 2003, 15: 162-166.
[8]
Zhong NY , Zhang WT , Tang DF et al . Fingerprint method and multi-component quantification analysis method study of Leonurus liquid extract[J]. Chin J Mod Appl Pharm (中国现代应用药学), 2015, 32: 1467-1471.
[9]
He YL , Jiang WD , He SF et al . HPLC fingerprint of Yimucao injection[J]. Chin J Med Chem (药物分析杂志), 2007, 27: 1578-1581. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=ywfxzz200710021
[10]
Miao WJ , Yang L , Wu DR et al . Fingerprints of Leonurus heterophyllus injection with capillary electrophoresis[J]. Chin Tradit Herb Drugs (中草药), 2007, 38: 1171-1174. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=zcy200708017
[11]
Yang C , Bao XH , Geng Z et al . Fingerprint analysis of Yimucao injection[J]. Chin J Med Chem (药物分析杂志), 2016, 36: 1219-1224. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=ywfxzz201607012
[12]
Deng Z , Zhang F , Zhang J et al . Standard decoction of Leonuri Herba[J]. China J Chin Mater Med (中国中药杂志), 2017, 42: 3523-3529. http://d.old.wanfangdata.com.cn/Periodical/zgzyzz201718014
2019年第54卷第11期
PDF下载
403
170
引用本文
BibTeX
文章信息
doi: 10.16438/j.0513-4870.2019-0407
  • 接收时间：2019-05-24
  • 首发时间：2026-01-26
  • 出版时间：2019-11-12
补充材料
相关文章
文章信息
作者
出版历史
  • 收稿日期：2019-05-24
  • 修回日期：2019-07-04
基金
名贵中药资源可持续利用能力建设项目(2060302-1508-04)
国家科技重大专项“重大新药创制”项目(2018ZX09201-010)
作者信息
    1.浙江大学药学院, 浙江 杭州 310058
    2.绍兴市食品药品检验研究院, 浙江 绍兴 312071

通讯作者:

*吴永江 ,Tel: 86-571-88208455, E-mail:
参考文献
分享链接
https://castjournals.cast.org.cn/joweb/yxxb/CN/10.16438/j.0513-4870.2019-0407
分享至
全文二维码

扫描看全文

引用本文
BibTeX
本文的引用情况
2种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
关闭全屏
相关链接
论文
最新文章
热读文章
热点专题
内参
蓝皮书
产业发展报告
传播力报告
期刊分级目录
资讯
学术新闻
行业新闻
学术会议
行业会议
关于
关于我们
联系我们
北京市海淀区学院南路86号
100081
010-62199257（仅加盟咨询）
qkjq@cast.org.cn

中国科学技术协会版权所有 京ICP 备10216604号-4 海淀分局备案 1101084647
科技导报社主办 中国科协信息中心技术支持