Article(id=1210517371735904317, tenantId=1146029695717560320, journalId=1189982191388893191, issueId=1210517366081975259, articleNumber=null, orderNo=null, doi=10.16438/j.0513-4870.2022-0129, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=research-article, receivedDate=1643212800000, receivedDateStr=2022-01-27, revisedDate=1652112000000, revisedDateStr=2022-05-10, acceptedDate=null, acceptedDateStr=null, onlineDate=1766539431747, onlineDateStr=2025-12-24, pubDate=1668182400000, pubDateStr=2022-11-12, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1766539431747, onlineIssueDateStr=2025-12-24, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1766539431747, creator=13701087609, updateTime=1766539431747, updator=13701087609, issue=Issue{id=1210517366081975259, tenantId=1146029695717560320, journalId=1189982191388893191, year='2022', volume='57', issue='11', pageStart='3259', pageEnd='3450', issueExtLink='null', onlineDate='null', pubDate='null', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1766539430399, creator=13701087609, updateTime=1766539608198, updator=13701087609, preIssue=null, nextIssue=null, ext={EN=IssueExt(id=1210518111875363690, tenantId=1146029695717560320, journalId=1189982191388893191, issueId=1210517366081975259, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1210518111875363691, tenantId=1146029695717560320, journalId=1189982191388893191, issueId=1210517366081975259, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null}, startPage=3316, endPage=3321, ext={EN=ArticleExt(id=1210517372146946122, articleId=1210517371735904317, tenantId=1146029695717560320, journalId=1189982191388893191, language=EN, title=Establishment and evaluation of
in vitro screen model for LTB4 receptor 1, columnId=1190335348761793317, journalTitle=Acta Pharmaceutica Sinica, columnName=Original Articles, runingTitle=null, highlight=null, articleAbstract=
The GPCR family component leukotriene B4 receptor 1 (LTB4R1) is the receptor of leukotriene B4 (LTB4), the metabolic product of ω6 fatty acid. LTB4R1 is a potential therapeutic target for the treatment of insulin resistance, chronic inflammation and type 2 diabetes. Here we established a LTB4R1 inhibitor screen model based on the GPCR family protein property that its activation causes the cytosolic escalation of calcium. The cytosolic calcium probe Fluo-8 represents the change of calcium ion. After adding LTB4, the fluorescent signal of Fluo-8 in the CHO cells which are co-transfected with LTB4R1 and Gα16 will change with the increase of cytosolic calcium, and LTB4R1 inhibitor blocked the effect of LTB4 on fluorescent signal of Fluo-8 in the CHO cells. Here, we used 0.2% DMSO as a negative control, and cp-105696 as a positive control in the screen model. After stimulation with LTB4, the Fluo-8 signal in 0.2% DMSO treated CHO cells increased 2 fold and fell back slowly, while the signal in inhibitor (cp-105696) treated cells was not induced by LTB4. The results showed that LTB4 increased the cytosolic calcium detected by Fluo-8 in a dose dependent manner. Similarly, cp-105696 inhibited the Fluo-8 signal dose dependently, indicating that this method can quantify the inhibitory activity of the compounds. The Z'-factor, reflecting the robustness of the screen model, was 0.777 with a series of experiments. In sum, we over-expressed LTB4R1α and Gα16 in CHO cell, used Fluo-8 to detect the calcium signal activated by LTB4, and established the in vitro screen model for LTB4 receptor 1.
, correspAuthors=Ping-ping LI, authorNote=null, correspAuthorsNote=null, copyrightStatement=Copyright ©2022 Acta Pharmaceutica Sinica. All rights reserved., copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, authorCompany=null, fund=null, authors=null, authorsList=Chun-xiao MA, Yan-jun WAN, Shao-cong HOU, Shu-wang HE, Shi-qiang YAN, Ping-ping LI), CN=ArticleExt(id=1210517374541893753, articleId=1210517371735904317, tenantId=1146029695717560320, journalId=1189982191388893191, language=CN, title=LTB4R1抑制剂筛选方法的建立及评价, columnId=1190335348896011050, journalTitle=药学学报, columnName=研究论文, runingTitle=null, highlight=null, articleAbstract=
ω6脂肪酸代谢产物白三烯B4 (LTB4) 的受体白三烯B4受体1 (LTB4R1) 属于G蛋白耦联受体(GPCR) 家族, 是胰岛素抵抗、慢性炎症和2型糖尿病的潜在药物作用靶点。本文根据GPCR家族蛋白激活后可以引起细胞质内钙离子升高的原理, 建立了96孔板体系LTB4R1抑制剂筛选模型。胞质内钙离子探针Fluo-8可以表征细胞质内的钙离子变化, 当共转染LTB4R1和G蛋白α亚基16 (Gα16) 的仓鼠卵巢癌细胞(CHO) 受到LTB4刺激后, 胞浆钙离子浓度升高, Fluo-8的荧光信号随之增强。LTB4R1抑制剂处理细胞后, Fluo-8的荧光信号减弱。本文以0.2% DMSO为阴性对照, LTB4R1抑制剂cp-105696为阳性对照建立筛选模型。经LTB4刺激后, 0.2% DMSO处理的细胞Fluo-8信号上升约2倍后缓慢回落, 而cp-105696处理组则抑制LTB4的作用。LTB4和抑制剂cp-105696浓度梯度实验表明, Fluo-8的荧光信号升高幅度和抑制剂的浓度成反比, 可以作为评价待测化合物的抑制效果的指标。多次重复实验计算所得阳性与阴性对照Z'因子为0.777, 证明该筛选模型稳定可靠。本文采用蛋白过表达方式在CHO细胞中构建了LTB4R1-内质网钙的信号通路, 并在此基础上建立了通过检测钙信号强度来筛选化合物对LTB4R1抑制活性的体系。本方法可用于LTB4R1抑制剂先导化合物的体外初步筛选, 为后续体内研究奠定基础。
, correspAuthors=李平平, authorNote=null, correspAuthorsNote=
, copyrightStatement=版权所有©《药学学报》编辑部2022, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=BTzWYuw9QwB9XIMJo0dq5A==, magXml=w+tdAWCQ7u6FDr/fOfJK6g==, pdfUrl=null, pdf=uw/PuhfWwLg32msjibTM8A==, pdfFileSize=2960775, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=4RcNt+hKTbcvcRtv5imfzg==, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=DfITNpi00a+0pHs6heWSmw==, mapNumber=null, authorCompany=null, fund=null, authors=null, authorsList=马春晓, 万彦军, 侯少聪, 何淑旺, 颜世强, 李平平)}, authors=[Author(id=1210517374999072923, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1210517371735904317, orderNo=0, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1210517375129096355, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1210517371735904317, authorId=1210517374999072923, language=EN, stringName=Chun-xiao MA, firstName=Chun-xiao, middleName=null, lastName=MA, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=
1, address=1. State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1210517375259119793, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1210517371735904317, authorId=1210517374999072923, language=CN, stringName=马春晓, firstName=春晓, middleName=null, lastName=马, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=
1, address=1.中国医学科学院、北京协和医学院药物研究所, 天然药物活性物质与功能国家重点实验室, 北京 100050, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1210517374780969098, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1210517371735904317, xref=null, ext=[AuthorCompanyExt(id=1210517374789357708, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1210517371735904317, companyId=1210517374780969098, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1. State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China), AuthorCompanyExt(id=1210517374797746317, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1210517371735904317, companyId=1210517374780969098, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1.中国医学科学院、北京协和医学院药物研究所, 天然药物活性物质与功能国家重点实验室, 北京 100050)])]), Author(id=1210517375452057792, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1210517371735904317, orderNo=1, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1210517375565304010, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1210517371735904317, authorId=1210517375452057792, language=EN, stringName=Yan-jun WAN, firstName=Yan-jun, middleName=null, lastName=WAN, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=
1, address=1. State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1210517375644995797, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1210517371735904317, authorId=1210517375452057792, language=CN, stringName=万彦军, firstName=彦军, middleName=null, lastName=万, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=
1, address=1.中国医学科学院、北京协和医学院药物研究所, 天然药物活性物质与功能国家重点实验室, 北京 100050, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1210517374780969098, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1210517371735904317, xref=null, ext=[AuthorCompanyExt(id=1210517374789357708, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1210517371735904317, companyId=1210517374780969098, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1. State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China), AuthorCompanyExt(id=1210517374797746317, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1210517371735904317, companyId=1210517374780969098, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1.中国医学科学院、北京协和医学院药物研究所, 天然药物活性物质与功能国家重点实验室, 北京 100050)])]), Author(id=1210517375762436319, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1210517371735904317, orderNo=2, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1210517375963762925, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1210517371735904317, authorId=1210517375762436319, language=EN, stringName=Shao-cong HOU, firstName=Shao-cong, middleName=null, lastName=HOU, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=
1, address=1. State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1210517376106369275, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1210517371735904317, authorId=1210517375762436319, language=CN, stringName=侯少聪, firstName=少聪, middleName=null, lastName=侯, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=
1, address=1.中国医学科学院、北京协和医学院药物研究所, 天然药物活性物质与功能国家重点实验室, 北京 100050, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1210517374780969098, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1210517371735904317, xref=null, ext=[AuthorCompanyExt(id=1210517374789357708, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1210517371735904317, companyId=1210517374780969098, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1. State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China), AuthorCompanyExt(id=1210517374797746317, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1210517371735904317, companyId=1210517374780969098, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1.中国医学科学院、北京协和医学院药物研究所, 天然药物活性物质与功能国家重点实验室, 北京 100050)])]), Author(id=1210517376194449668, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1210517371735904317, orderNo=3, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1210517376282530061, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1210517371735904317, authorId=1210517376194449668, language=EN, stringName=Shu-wang HE, firstName=Shu-wang, middleName=null, lastName=HE, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=
2, address=2. Shandong DYNE Marine Biopharmaceutical Co., Ltd., Weihai 264333, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1210517376387387672, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1210517371735904317, authorId=1210517376194449668, language=CN, stringName=何淑旺, firstName=淑旺, middleName=null, lastName=何, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=
2, address=2.山东达因海洋生物制药有限公司, 山东 威海 264333, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1210517374898409620, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1210517371735904317, xref=null, ext=[AuthorCompanyExt(id=1210517374906798230, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1210517371735904317, companyId=1210517374898409620, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2. Shandong DYNE Marine Biopharmaceutical Co., Ltd., Weihai 264333, China), AuthorCompanyExt(id=1210517374915186838, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1210517371735904317, companyId=1210517374898409620, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2.山东达因海洋生物制药有限公司, 山东 威海 264333)])]), Author(id=1210517376462885155, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1210517371735904317, orderNo=4, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1210517376529994027, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1210517371735904317, authorId=1210517376462885155, language=EN, stringName=Shi-qiang YAN, firstName=Shi-qiang, middleName=null, lastName=YAN, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=
2, address=2. Shandong DYNE Marine Biopharmaceutical Co., Ltd., Weihai 264333, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1210517376643240246, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1210517371735904317, authorId=1210517376462885155, language=CN, stringName=颜世强, firstName=世强, middleName=null, lastName=颜, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=
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J Clin Invest,
2003,
112: 1796-1808., articleTitle=Obesity is associated with macrophage accumulation in adipose tissue, refAbstract=null), Reference(id=1210517381126951496, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1210517371735904317, doi=10.2337/db09-0287, pmid=null, pmcid=null, year=2010, volume=59, issue=null, pageStart=1648, pageEnd=1656, url=null, language=null, rfNumber=[2], rfOrder=1, authorNames=null, journalName=Diabetes, refType=null, unstructuredReference=Wentworth JM, Naselli G, Brown WA, et al. Pro-inflammatory CD11c
+CD206
+ adipose tissue macrophages are associated with insulin resistance in human obesity[J].
Diabetes,
2010,
59: 1648-1656., articleTitle=Pro-inflammatory CD11c
+CD206
+ adipose tissue macrophages are associated with insulin resistance in human obesity, refAbstract=null), Reference(id=1210517381215031888, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1210517371735904317, doi=10.1152/physrev.00063.2017, pmid=null, pmcid=null, year=2018, volume=98, issue=null, pageStart=2133, pageEnd=2223, url=null, language=null, rfNumber=[3], rfOrder=2, authorNames=null, journalName=Physiol Rev, refType=null, unstructuredReference=Petersen MC, Shulman GI. Mechanisms of insulin action and insulin resistance[J].
Physiol Rev,
2018,
98: 2133-2223., articleTitle=Mechanisms of insulin action and insulin resistance, refAbstract=null), Reference(id=1210517381328278102, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1210517371735904317, doi=10.1038/nm.3800, pmid=null, pmcid=null, year=2015, volume=21, issue=null, pageStart=239, pageEnd=247, url=null, language=null, rfNumber=[4], rfOrder=3, authorNames=null, journalName=Nat Med, refType=null, unstructuredReference=Li P, Oh DY, Bandyopadhyay G, et al. LTB4 promotes insulin resistance in obese mice by acting on macrophages, hepatocytes and myocytes[J].
Nat Med,
2015,
21: 239-247., articleTitle=LTB4 promotes insulin resistance in obese mice by acting on macrophages, hepatocytes and myocytes, refAbstract=null), Reference(id=1210517382565597790, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1210517371735904317, doi=10.1016/S0952-3278(03)00073-5, pmid=null, pmcid=null, year=2003, volume=69, issue=null, pageStart=123, pageEnd=134, url=null, language=null, rfNumber=[5], rfOrder=4, authorNames=null, journalName=Prostaglandins Leukot Essent Fatty Acids, refType=null, unstructuredReference=Tager AM, Luster AD. BLT1 and BLT2: the leukotriene B4 receptors[J].
Prostaglandins Leukot Essent Fatty Acids,
2003,
69: 123-134., articleTitle=BLT1 and BLT2: the leukotriene B4 receptors, refAbstract=null), Reference(id=1210517382670455396, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1210517371735904317, doi=10.1038/s41467-022-29073-2, pmid=null, pmcid=null, year=2022, volume=13, issue=null, pageStart=1408, pageEnd=null, url=null, language=null, rfNumber=[6], rfOrder=5, authorNames=null, journalName=Nat Commun, refType=null, unstructuredReference=Schmitz EA, Takahashi H, Karakas E. Structural basis for activation and gating of IP3 receptors[J].
Nat Commun,
2022,
13: 1408., articleTitle=Structural basis for activation and gating of IP3 receptors, refAbstract=null), Reference(id=1210517382825644651, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1210517371735904317, doi=10.1085/jgp.201210886, pmid=null, pmcid=null, year=2013, volume=141, issue=null, pageStart=521, pageEnd=535, url=null, language=null, rfNumber=[7], rfOrder=6, authorNames=null, journalName=J Gen Physiol, refType=null, unstructuredReference=Dickson EJ, Falkenburger BH, Hille B. Quantitative properties and receptor reserve of the IP3 and calcium branch of Gq-coupled receptor signaling[J].
J Gen Physiol,
2013,
141: 521-535., articleTitle=Quantitative properties and receptor reserve of the IP3 and calcium branch of Gq-coupled receptor signaling, refAbstract=null), Reference(id=1210517382951473782, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1210517371735904317, doi=10.1080/17460441.2017.1303473, pmid=null, pmcid=null, year=2017, volume=12, issue=null, pageStart=511, pageEnd=523, url=null, language=null, rfNumber=[8], rfOrder=7, authorNames=null, journalName=Expert Opin Drug Discov, refType=null, unstructuredReference=Ma Q, Ye L, Liu H, et al. An overview of Ca
2+ mobilization assays in GPCR drug discovery[J].
Expert Opin Drug Discov,
2017,
12: 511-523., articleTitle=An overview of Ca
2+ mobilization assays in GPCR drug discovery, refAbstract=null), Reference(id=1210517383035359870, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1210517371735904317, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[9], rfOrder=8, authorNames=null, journalName=null, refType=null, unstructuredReference=Arkin MR, Connor PR, Emkey R, et al. FLIPR
TM Assays for GPCR and ion channel targets [M] // Markossian S, Grossman A, Brimacombe K, et al. Assay Guidance Manual. Bethesda (MD): Eli Lilly & Company and the National Center for Advancing Translational Sciences, 2012: 1-33., articleTitle=null, refAbstract=null), Reference(id=1210517383123440259, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1210517371735904317, doi=10.4049/jimmunol.179.8.5454, pmid=null, pmcid=null, year=2007, volume=179, issue=null, pageStart=5454, pageEnd=5461, url=null, language=null, rfNumber=[10], rfOrder=9, authorNames=null, journalName=J Immunol, refType=null, unstructuredReference=Peres CM, Aronoff DM, Serezani CH, et al. Specific leukotriene receptors couple to distinct G proteins to effect stimulation of alveolar macrophage host defense functions[J].
J Immunol,
2007,
179: 5454-5461., articleTitle=Specific leukotriene receptors couple to distinct G proteins to effect stimulation of alveolar macrophage host defense functions, refAbstract=null), Reference(id=1210517383207326345, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1210517371735904317, doi=10.1177/1087057102239665, pmid=null, pmcid=null, year=2003, volume=8, issue=null, pageStart=39, pageEnd=49, url=null, language=null, rfNumber=[11], rfOrder=10, authorNames=null, journalName=J Biomol Screen, refType=null, unstructuredReference=Liu AM, Ho MK, Wong CS, et al. G
α(16/z) chimeras efficiently link a wide range of G protein-coupled receptors to calcium mobilization[J].
J Biomol Screen,
2003,
8: 39-49., articleTitle=G
α(16/z) chimeras efficiently link a wide range of G protein-coupled receptors to calcium mobilization, refAbstract=null), Reference(id=1210517383307989649, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1210517371735904317, doi=null, pmid=null, pmcid=null, year=1995, volume=273, issue=null, pageStart=176, pageEnd=184, url=null, language=null, rfNumber=[12], rfOrder=11, authorNames=null, journalName=J Pharmacol Exp Ther, refType=null, unstructuredReference=Showell HJ, Pettipher ER, Cheng JB, et al. The
in vitro and
in vivo pharmacologic activity of the potent and selective leukotriene B4 receptor antagonist CP-105696[J].
J Pharmacol Exp Ther,
1995,
273: 176-184., articleTitle=The
in vitro and
in vivo pharmacologic activity of the potent and selective leukotriene B4 receptor antagonist CP-105696, refAbstract=null), Reference(id=1210517383513510551, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1210517371735904317, doi=10.1038/nature10867, pmid=null, pmcid=null, year=2012, volume=482, issue=null, pageStart=552, pageEnd=556, url=null, language=null, rfNumber=[13], rfOrder=12, authorNames=null, journalName=Nature, refType=null, unstructuredReference=Kruse AC, Hu J, Pan AC, et al. Structure and dynamics of the M3 muscarinic acetylcholine receptor[J].
Nature,
2012,
482: 552-556., articleTitle=Structure and dynamics of the M3 muscarinic acetylcholine receptor, refAbstract=null), Reference(id=1210517383626756767, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1210517371735904317, doi=null, pmid=null, pmcid=null, year=2017, volume=142, issue=null, pageStart=51, pageEnd=57, url=null, language=null, rfNumber=[14], rfOrder=13, authorNames=null, journalName=Methods Cell Biol, refType=null, unstructuredReference=Vasudevan NT. cAMP assays in GPCR drug discovery[J].
Methods Cell Biol,
2017,
142: 51-57., articleTitle=cAMP assays in GPCR drug discovery, refAbstract=null), Reference(id=1210517383723225765, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1210517371735904317, doi=10.1038/nmeth.3398, pmid=null, pmcid=null, year=2015, volume=12, issue=null, pageStart=661, pageEnd=663, url=null, language=null, rfNumber=[15], rfOrder=14, authorNames=null, journalName=Nat Methods, refType=null, unstructuredReference=Stoddart LA, Johnstone EKM, Wheal AJ, et al. Application of BRET to monitor ligand binding to GPCRs[J].
Nat Methods,
2015,
12: 661-663., articleTitle=Application of BRET to monitor ligand binding to GPCRs, refAbstract=null)], funds=[Fund(id=1210517380648800798, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1210517371735904317, awardId=81770800, language=CN, fundingSource=国家自然科学基金资助项目(81770800), fundOrder=null, country=null), Fund(id=1210517380736881189, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1210517371735904317, awardId=2021-I2M-1-016, language=CN, fundingSource=中国医学科学院医学与健康科技创新工程(2021-I2M-1-016), fundOrder=null, country=null), Fund(id=1210517380854321711, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1210517371735904317, awardId=2018RC350004, language=CN, fundingSource=中国医学科学院中央级公益性科研院所基本科研业务费(2018RC350004), fundOrder=null, country=null), Fund(id=1210517380954985014, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1210517371735904317, awardId=BJJWZYJH01201910023028, language=CN, fundingSource=北京高校卓越青年科学家计划项目(BJJWZYJH01201910023028), fundOrder=null, country=null)], companyList=[AuthorCompany(id=1210517374780969098, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1210517371735904317, xref=null, ext=[AuthorCompanyExt(id=1210517374789357708, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1210517371735904317, companyId=1210517374780969098, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1. State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China), AuthorCompanyExt(id=1210517374797746317, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1210517371735904317, companyId=1210517374780969098, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1.中国医学科学院、北京协和医学院药物研究所, 天然药物活性物质与功能国家重点实验室, 北京 100050)]), AuthorCompany(id=1210517374898409620, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1210517371735904317, xref=null, ext=[AuthorCompanyExt(id=1210517374906798230, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1210517371735904317, companyId=1210517374898409620, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2. Shandong DYNE Marine Biopharmaceutical Co., Ltd., Weihai 264333, China), AuthorCompanyExt(id=1210517374915186838, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1210517371735904317, companyId=1210517374898409620, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2.山东达因海洋生物制药有限公司, 山东 威海 264333)])], figs=[ArticleFig(id=1210517379327594948, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1210517371735904317, language=EN, label=null, caption=null, figureFileSmall=EGRDF01t3MRXxMs2vSqdFA==, figureFileBig=4RcNt+hKTbcvcRtv5imfzg==, tableContent=null), ArticleFig(id=1210517379436646855, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1210517371735904317, language=CN, label=Figure 1, caption=
LTB4 stimulated calcium transient in CHO cells transfected with LTB4R1 and G<i>α</i>16. A: Leukotriene B4 receptor 1 (LTB4R1) and G<i>α</i>16 expression pattern by Western blot after transfection with LTB4R1 and G<i>α</i>16 for 0, 24, 36, 48, and 72 h in CHO cells; B: The increase of calcium transient with leukotriene B4 (LTB4, 400 nmol‧L<sup>-1</sup>) stimulation by Fluo-8 calcium probe in CHO cells transfected with LTB4R1, G<i>α</i>16, or both; C: Immunofluorescence of the Fluo-8 calcium probe in CHO cells with LTB4 stimulation. Fluo-8 calcium probe detects cytosolic calcium and expresses green fluorescence. RFU: Relative fluorescence units , figureFileSmall=EGRDF01t3MRXxMs2vSqdFA==, figureFileBig=4RcNt+hKTbcvcRtv5imfzg==, tableContent=null), ArticleFig(id=1210517379705082332, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1210517371735904317, language=EN, label=null, caption=null, figureFileSmall=gHGNgLMv1GoipMVQCJ5bZg==, figureFileBig=78HreiEfYgFftdK29ZAWQw==, tableContent=null), ArticleFig(id=1210517379885437415, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1210517371735904317, language=CN, label=Figure 2, caption=
LTB4 induced calcium change dose dependently in CHO cells transfected with LTB4R1 and G<i>α</i>16. A, B: Effect of 400 nmol‧L<sup>-1</sup> LTB4 on intracellular calcium concentration using Fluo-8 calcium probe in LTB4R1 and G<i>α</i>16 co-transfected CHO cells; C: The calcium signal by Fluo-8 calcium probe in LTB4R1 and G<i>α</i>16 co-transfected CHO cells with stimulation of LTB4 from 100 pmol‧L<sup>-1</sup> to 10 µmol‧L<sup>-1</sup>; D: The LTB4 activation curve in CHO cells. <i>n</i> = 3, <span class="mag-xml-inline-formula">$ \overline{x} $</span> ± SEM in D , figureFileSmall=gHGNgLMv1GoipMVQCJ5bZg==, figureFileBig=78HreiEfYgFftdK29ZAWQw==, tableContent=null), ArticleFig(id=1210517380036432367, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1210517371735904317, language=EN, label=null, caption=null, figureFileSmall=oj1VzOtksGOKlCXcFxNO7A==, figureFileBig=tJf9OKBI7DxVpIsrDi+F9w==, tableContent=null), ArticleFig(id=1210517380187427324, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1210517371735904317, language=CN, label=Figure 3, caption=
LTB4R1 inhibitor cp-105696 blocked the calcium transient in CHO cells. A, B: Overnight treatment of 10 μmol‧L<sup>-1</sup> cp-105696 inhibited the calcium transient by Fluo-8 calcium probe in LTB4R1 and G<i>α</i>16 co-transfected CHO cells; C: The calcium fluctuation in LTB4R1 and G<i>α</i>16 transfected CHO cells with cp-105696 (1 pmol‧L<sup>-1</sup> to 100 μmol‧L<sup>-1</sup>, overnight); D: The inhibition curve of cp-105696 in CHO cells. <i>n</i> = 4, <span class="mag-xml-inline-formula">$ \overline{x} $</span> ± SEM in D , figureFileSmall=oj1VzOtksGOKlCXcFxNO7A==, figureFileBig=tJf9OKBI7DxVpIsrDi+F9w==, tableContent=null), ArticleFig(id=1210517380317450757, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1210517371735904317, language=EN, label=null, caption=null, figureFileSmall=4NZco25edQLbjjioibeflw==, figureFileBig=JJXuLCbnX5zCNdkIp8s6fA==, tableContent=null), ArticleFig(id=1210517380502000145, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1210517371735904317, language=CN, label=Figure 4, caption=
The stability and reliability of LTB4R1 screening model. A: The increase of fluorescence induced by 400 nmol‧L<sup>-1</sup> LTB4 in CHO cells after vehicle and 10 μmol‧L<sup>-1</sup> cp-105696 treatment respectively; B. The increase of fluorescence induced by 400 nmol‧L<sup>-1</sup> LTB4 at different time points after 30 mins of Fluo-8 calcium probe incubation in CHO cells. Increase = (peak-basal)/basal × 100%. <i>n</i> =10, <span class="mag-xml-inline-formula">$ \overline{x} $</span> ± SEM in A. <sup>***</sup><i>P</i> < 0.001. , figureFileSmall=4NZco25edQLbjjioibeflw==, figureFileBig=JJXuLCbnX5zCNdkIp8s6fA==, tableContent=null)], attaches=null, journal=Journal(id=1189982048455397383, delFlag=0, nameCn=药学学报, nameEn=Acta Pharmaceutica Sinica, nameHistory1=null, nameHistory2=null, issn=0513-4870, eissn=null, cn=11-2163/R, coden=null, periodic=0, language=CN, oaType=null, ccby=null, superviseOffice=null, ownerOffice=null, pubOffice=null, editorOffice=null, officeType=null, aims=null, clcCode=null, officeProv=null, officeCity=null, officeAddr=null, officeZip=null, officeEmail=null, officePhone=null, editDirector=null, officeDirector=null, officeDirectorPhone=null, officeStaffNum=null, officeEmpNum=null, coverPicUrl=BTxjudbJDVO4PqdBR6On6Q==, journalPrice=null, startedYear=null, abbrevIsoEn=null, journalRemark=null, publicationField=null, 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