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Article(id=1210147888731132404, tenantId=1146029695717560320, journalId=1189982191388893191, issueId=1210147879319113875, articleNumber=null, orderNo=null, doi=10.16438/j.0513-4870.2022-0123, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=research-article, receivedDate=1643126400000, receivedDateStr=2022-01-26, revisedDate=1646668800000, revisedDateStr=2022-03-08, acceptedDate=null, acceptedDateStr=null, onlineDate=1766451340134, onlineDateStr=2025-12-23, pubDate=1654963200000, pubDateStr=2022-06-12, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1766451340134, onlineIssueDateStr=2025-12-23, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1766451340134, creator=13701087609, updateTime=1766451340134, updator=13701087609, issue=Issue{id=1210147879319113875, tenantId=1146029695717560320, journalId=1189982191388893191, year='2022', volume='57', issue='6', pageStart='1541', pageEnd='1924', issueExtLink='null', onlineDate='null', pubDate='null', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1766451337890, creator=13701087609, updateTime=1766451466252, updator=13701087609, preIssue=null, nextIssue=null, ext={EN=IssueExt(id=1210148417767084534, tenantId=1146029695717560320, journalId=1189982191388893191, issueId=1210147879319113875, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1210148417767084535, tenantId=1146029695717560320, journalId=1189982191388893191, issueId=1210147879319113875, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null}, startPage=1880, endPage=1886, ext={EN=ArticleExt(id=1210147889792291360, articleId=1210147888731132404, tenantId=1146029695717560320, journalId=1189982191388893191, language=EN, title=Simultaneous determination of 10 components in Guanxinshutong capsules by quantitative analysis of multi-components by single marker, columnId=1190335348761793317, journalTitle=Acta Pharmaceutica Sinica, columnName=Original Articles, runingTitle=null, highlight=null, articleAbstract=

This study establishes a quantitative analysis of multi-components by single marker (QAMS) method for the simultaneous determination of gallic acid, sodium danshensu, protocatechuic acid, protocatechuic aldehyde, vanillin, rosmarinic acid, salvianolic acid B, eugenol, cryptotanshinone and tanshinone IIA in Guanxinshutong capsules (Bambusae Concretio Silicea, Salvia miltiorrhiza, clove, borneol, Bambusae Concretio Silicea) by HPLC. Sample was loaded onto an Agilent C18 (ZORBAX Extend-RP C18, 250 mm × 4.6 mm, 5 µm) column and eluted with methanol-0.4% aqueous formic acid solution as a flow phase gradient, flow speed 1.0 mL·min-1, detection wavelength 280 nm, column temperature 35 ℃ and sample intake of 5 µL. Using protocatechuic acid as the internal reference, a relative correction factor was calculated and the durability was investigated, and the content of 10 components was calculated by QAMS and external standard method (ESM). The results show that the specificity, linear relationship, precision, repeatability, and stability of the 10 components were good. The average recovery was 98.20%-103.47% and RSD was 1.26%-2.84%. The relative positive factors and contents of the other nine components were calculated as gallic acid (0.759, 227.381), sodium tanshinol (3.630, 3.283), protocatechualdehyde (0.185, 0.150), vanillin (0.532, 65.213), rosmarinic acid (4.240, 1.035), salvianolic acid B (3.245, 18.204), eugenol (1.729, 9.265), cryptotanshinone (0.691, 1.449), and tanshinone ⅡA (0.702, 1.939). The results of QAMS were consistent with ESM analysis, and the relative error was between -3% and 3%. This method is stable and reliable, and can be used for the determination of 10 components in Guanxinshutong capsules.

, correspAuthors=Ye LI, authorNote=null, correspAuthorsNote=null, copyrightStatement=Copyright ©2022 Acta Pharmaceutica Sinica. All rights reserved., copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, authorCompany=null, fund=null, authors=null, authorsList=Kai-hua LONG, Feng LIU, Hong ZHANG, Xia DU, Chun-liu WANG, Yang LIU, Dong-hua YANG, Ye LI), CN=ArticleExt(id=1210147890467574355, articleId=1210147888731132404, tenantId=1146029695717560320, journalId=1189982191388893191, language=CN, title=一测多评法同时测定冠心舒通胶囊中10个成分的含量, columnId=1190335348896011050, journalTitle=药学学报, columnName=研究论文, runingTitle=null, highlight=null, articleAbstract=

基于一测多评法建立高效液相色谱法同时测定冠心舒通胶囊(广枣、丹参、丁香、冰片、天竺黄) 中没食子酸、丹参素钠、原儿茶酸、原儿茶醛、香草醛、迷迭香酸、丹酚酸B、丁香酚、隐丹参酮、丹参酮ⅡA的含量。采用Agilent C18 (ZORBAX Extend-RP C18, 250 mm × 4.6 mm, 5 µm) 色谱柱, 以甲醇-0.4%甲酸水溶液为流动相梯度洗脱, 流速1.0 mL·min-1, 检测波长280 nm, 柱温35 ℃, 进样量5 µL。以原儿茶酸为内参物, 计算相对校正因子并进行耐用性考察, 通过一测多评法和外标法计算10个成分的含量。结果表明, 10种成分专属性、线性关系、精密度、重复性、稳定性均良好, 平均加样回收率98.20%~103.47%, RSD 1.26%~2.84%。计算其他9个成分的相对校正因子、含量(mg·g-1) 分别为没食子酸(0.759, 227.381)、丹参素钠(3.630, 3.283)、原儿茶醛(0.185, 0.150)、香草醛(0.532, 65.213)、迷迭香酸(4.240, 1.035)、丹酚酸B (3.245, 18.204)、丁香酚(1.729, 9.265)、隐丹参酮(0.691, 1.449)、丹参酮ⅡA (0.702, 1.939)。一测多评法所得结果与外标法接近, 相对误差在-3%~3%之间。表明该方法稳定可靠, 可用于冠心舒通胶囊中10个成分的含量测定。

, correspAuthors=李晔, authorNote=null, correspAuthorsNote=
*李晔, Tel: 86-29-87251837, E-mail:
, copyrightStatement=版权所有©《药学学报》编辑部2022, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=EvvfiHeiWqIZQlDPNG1oBg==, magXml=1/UklY8y1NWuVU2YPuWMFg==, pdfUrl=null, pdf=He3cPNJ9cNaoelZ5ZJWgng==, pdfFileSize=403983, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=2Stz4b/ZGV8FTmnno2tQIg==, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=dRqV2hUNf+cAfKec5W7VqQ==, mapNumber=null, authorCompany=null, fund=null, authors=null, authorsList=龙凯花, 刘峰, 张红, 杜霞, 王春柳, 刘洋, 杨东花, 李晔)}, authors=[Author(id=1210147891423875763, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1210147888731132404, orderNo=0, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1210147891528733377, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1210147888731132404, authorId=1210147891423875763, language=EN, stringName=Kai-hua LONG, firstName=Kai-hua, middleName=null, lastName=LONG, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, address=1. Shaanxi Academy of Traditional Chinese Medicine, Xi'an 710003, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1210147891608425162, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1210147888731132404, authorId=1210147891423875763, language=CN, stringName=龙凯花, firstName=凯花, middleName=null, lastName=龙, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, address=1.陕西省中医药研究院, 陕西 西安 710003, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1210147890761175658, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1210147888731132404, xref=null, ext=[AuthorCompanyExt(id=1210147890769564267, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1210147888731132404, companyId=1210147890761175658, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1. Shaanxi Academy of Traditional Chinese Medicine, Xi'an 710003, China), AuthorCompanyExt(id=1210147890803118708, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1210147888731132404, companyId=1210147890761175658, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1.陕西省中医药研究院, 陕西 西安 710003)])]), Author(id=1210147891746837210, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1210147888731132404, orderNo=1, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1210147891876860653, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1210147888731132404, authorId=1210147891746837210, language=EN, stringName=Feng LIU, firstName=Feng, middleName=null, lastName=LIU, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=2, 3, 4, address=2. Shaanxi Buchang Pharmaceutical Limited Company, Xi'an 710075, China
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AnalyteRegression equationR2Linear range /μg·mL-1
GAY = 11.596 x - 0.108 80.999 63.03-18.18
SDSSY = 2.345 8 x + 6.496 20.999 735.6-213.6
PCAY = 8.670 1 x + 1.944 60.999 66.96-41.76
PCAEY = 48.384 x - 2.909 50.999 31.16-6.96
VLY = 15.815 x + 1.356 30.999 20.868-5.21
RAY = 2.065 4 x - 0.572 70.999 329.526-177.154
SABY = 2.690 8 x + 8.177 90.999 9438.4-2630.4
EGY = 5.080 3 x - 5.625 00.999 7133.2-799.2
CTSY = 12.182 x + 15.1010.999 68.56-51.36
TSTY = 12.225 x + 36.3360.999 746-276
), ArticleFig(id=1210147896368959696, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1210147888731132404, language=CN, label=Table 1, caption=

Linear relationships of ten components

, figureFileSmall=null, figureFileBig=null, tableContent=
AnalyteRegression equationR2Linear range /μg·mL-1
GAY = 11.596 x - 0.108 80.999 63.03-18.18
SDSSY = 2.345 8 x + 6.496 20.999 735.6-213.6
PCAY = 8.670 1 x + 1.944 60.999 66.96-41.76
PCAEY = 48.384 x - 2.909 50.999 31.16-6.96
VLY = 15.815 x + 1.356 30.999 20.868-5.21
RAY = 2.065 4 x - 0.572 70.999 329.526-177.154
SABY = 2.690 8 x + 8.177 90.999 9438.4-2630.4
EGY = 5.080 3 x - 5.625 00.999 7133.2-799.2
CTSY = 12.182 x + 15.1010.999 68.56-51.36
TSTY = 12.225 x + 36.3360.999 746-276
), ArticleFig(id=1210147896486400225, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1210147888731132404, language=EN, label=null, caption=null, figureFileSmall=null, figureFileBig=null, tableContent=
InstrumentColumnf
fGA/PCAfSDSS/PCAfPCAE/PCAfVL/PCAfRA/PCAfSAB/PCAfEG/PCAfCTS/PCAfTST/PCA
Agilent 1260C10.7693.6290.1870.5324.2423.2671.7220.6830.704
C20.7603.6380.1840.5444.2733.1741.7460.6640.722
C30.7713.6210.1790.5324.2343.2221.7210.6650.744
Waters 2695C10.7553.6280.1860.5524.2643.2511.8030.7020.716
C20.7623.6340.1840.5324.2813.2671.7820.6720.722
C30.7743.6540.1850.5414.2403.2941.7540.6830.711
Average0.7653.6340.1840.5394.2563.2461.7550.6780.720
RSD/%0.9290.3081.5051.5420.4591.3011.8772.1061.902
), ArticleFig(id=1210147896608035049, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1210147888731132404, language=CN, label=Table 2, caption=

The relative correction factor (f) of different instruments and chromatographic columns (n = 3). C1: Kromasil C18; C2: Diamonsil C18; C3: Agilent ZORBAX Extend-C18

, figureFileSmall=null, figureFileBig=null, tableContent=
InstrumentColumnf
fGA/PCAfSDSS/PCAfPCAE/PCAfVL/PCAfRA/PCAfSAB/PCAfEG/PCAfCTS/PCAfTST/PCA
Agilent 1260C10.7693.6290.1870.5324.2423.2671.7220.6830.704
C20.7603.6380.1840.5444.2733.1741.7460.6640.722
C30.7713.6210.1790.5324.2343.2221.7210.6650.744
Waters 2695C10.7553.6280.1860.5524.2643.2511.8030.7020.716
C20.7623.6340.1840.5324.2813.2671.7820.6720.722
C30.7743.6540.1850.5414.2403.2941.7540.6830.711
Average0.7653.6340.1840.5394.2563.2461.7550.6780.720
RSD/%0.9290.3081.5051.5420.4591.3011.8772.1061.902
), ArticleFig(id=1210147896738058481, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1210147888731132404, language=EN, label=null, caption=null, figureFileSmall=null, figureFileBig=null, tableContent=
InstrumentColumntR
tGA/PCAtSDSS/PCAtPCAE/PCAtVL/PCAtRA/PCAtSAB/PCAtEG/PCAtCTS/PCAtTST/PCA
Agilent 1260C10.6470.9391.2431.7323.0803.3114.7767.0657.421
C20.6250.9381.2341.7173.0033.2644.8857.4417.817
C30.6450.9341.2321.7553.1413.3824.8947.4007.760
Waters 2695C10.6320.9221.2311.7183.1303.3014.8127.1897.411
C20.6170.9321.2421.7113.0523.1864.8727.4557.815
C30.6340.9121.2231.7213.1283.3634.8317.4197.712
Average0.6330.9291.2341.7263.0893.3014.8457.3287.656
RSD/%1.8131.1090.6080.9231.7622.1470.9542.2092.483
), ArticleFig(id=1210147896901636350, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1210147888731132404, language=CN, label=Table 3, caption=

Relative retention time determined by different instruments and columns (n = 3)

, figureFileSmall=null, figureFileBig=null, tableContent=
InstrumentColumntR
tGA/PCAtSDSS/PCAtPCAE/PCAtVL/PCAtRA/PCAtSAB/PCAtEG/PCAtCTS/PCAtTST/PCA
Agilent 1260C10.6470.9391.2431.7323.0803.3114.7767.0657.421
C20.6250.9381.2341.7173.0033.2644.8857.4417.817
C30.6450.9341.2321.7553.1413.3824.8947.4007.760
Waters 2695C10.6320.9221.2311.7183.1303.3014.8127.1897.411
C20.6170.9321.2421.7113.0523.1864.8727.4557.815
C30.6340.9121.2231.7213.1283.3634.8317.4197.712
Average0.6330.9291.2341.7263.0893.3014.8457.3287.656
RSD/%1.8131.1090.6080.9231.7622.1470.9542.2092.483
), ArticleFig(id=1210147897077797133, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1210147888731132404, language=EN, label=null, caption=null, figureFileSmall=null, figureFileBig=null, tableContent=
ComponentMethodS1S2S3S4S5S6S7S8S9S10S11S12S13S14S15S16S17S18S19S20
PCAESM0.4070.3290.4790.4830.5650.5620.3510.3980.3780.3810.4020.5550.5470.4450.4380.4770.4880.4310.4670.557
GAQAMS181.271287.284226.778219.914235.569228.139154.208190.281240.573258.270255.446215.742212.938295.892210.868217.165230.656206.914229.191217.284
ESM184.091287.350221.767223.912240.461233.833155.122193.599246.660254.182257.336219.053215.515301.985209.565217.125229.322207.548226.342222.842
RE (%)-1.532-0.0232.260-1.786-2.034-2.435-0.589-1.714-2.4681.608-0.734-1.511-1.196-2.0180.6220.0180.582-0.3051.258-2.494
SDSSQAMS3.0203.6513.6673.5762.6312.5123.4513.2316.0503.2243.2162.7002.5983.0353.3953.2403.5643.1583.3682.725
ESM2.9963.5993.5913.5682.6482.5693.3953.2446.1013.1363.1802.7762.5942.9983.2973.1843.4903.2403.3252.723
RE (%)0.8231.4542.1180.247-0.642-2.2331.655-0.412-0.8332.8181.139-2.7500.1501.2452.9431.7512.116-2.5231.2880.038
PCAEQAMS0.1280.1740.1550.1810.1100.1080.1760.1600.2830.1530.1490.1220.1210.1300.1420.1400.1480.1300.1340.127
ESM0.1290.1740.1540.1840.1130.1110.1750.1650.2890.1510.1500.1230.1210.1310.1380.1380.1460.1340.1380.128
RE (%)-0.412-0.1261.262-1.648-2.861-2.8260.678-2.936-2.2081.146-0.478-0.357-0.510-0.4682.2311.3501.422-2.648-2.710-0.258
VLQAMS72.72734.19979.17273.75366.40265.85256.23973.74833.31531.51235.52982.14583.17240.10272.48677.28384.46976.08279.75984.189
ESM73.16335.16578.49575.62465.14064.31957.05473.67934.23632.44336.13182.11684.47341.43474.55976.03982.65674.08077.52185.938
RE (%)-0.596-2.7460.862-2.4741.9382.384-1.4300.094-2.689-2.872-1.6660.035-1.5391.662-2.7811.6362.1932.7022.887-2.035
RAQAMS0.9850.9731.0951.1281.3001.1920.8320.9570.8260.9700.9740.8800.8180.9101.1061.0631.2051.0171.1560.854
ESM1.0121.0031.1261.1601.3321.2200.8560.9830.8510.9741.0020.9040.8420.9361.1311.0941.2310.9871.1850.876
RE (%)-2.657-2.967-2.749-2.746-2.374-2.275-2.850-2.608-2.985-0.451-2.810-2.716-2.905-2.791-2.160-2.826-2.0982.967-2.450-2.427
SABQAMS20.18117.54619.90417.92617.73516.92215.33517.08811.99016.86816.65714.63114.50318.54821.76020.53123.02821.24721.54614.558
ESM20.80317.76220.02018.44717.78617.43515.59517.47812.10516.77416.77614.98714.86618.69721.82420.89523.10121.88521.91814.919
RE (%)-2.989-1.215-0.584-2.827-0.285-2.944-1.666-2.233-0.9480.557-0.713-2.374-2.442-0.800-0.296-1.746-0.315-2.914-1.694-2.420
EGQAMS8.26310.5275.2763.2309.1139.81911.2787.86710.20511.73711.6528.7038.86812.1568.5538.8549.5008.6318.6778.773
ESM8.46010.5815.3983.3249.3149.90811.6218.0499.97812.09711.9188.8849.07712.5108.8079.1129.6168.8778.7808.986
RE (%)-2.330-0.507-2.249-2.825-2.151-0.899-2.953-2.2692.278-2.975-2.229-2.033-2.308-2.836-2.885-2.779-1.205-2.778-1.173-2.371
CTSQAMS1.4560.9361.3111.3811.1611.1811.3851.2591.4391.1441.5011.3971.7441.7501.3471.4761.5241.4181.6102.180
ESM1.4970.9311.3181.3951.1751.1921.3931.2921.4581.1491.5271.4051.7781.8001.3761.5181.5101.4501.5652.243
RE (%)-2.7600.492-0.560-1.004-1.216-0.920-0.560-2.561-1.322-0.363-1.670-0.612-1.929-2.747-2.137-2.7550.919-2.2052.872-2.795
TSTQAMS2.1681.1151.7251.7771.3131.3041.7801.6801.8801.5822.1111.7332.2692.2772.0112.0422.0592.0142.2513.017
ESM2.2301.1181.7541.8171.3131.3131.8011.7211.9191.5952.1441.7832.3172.3412.0682.1042.1022.0502.1913.105
RE (%)-2.758-0.311-1.667-2.176-0.738-0.738-1.1321.733-2.038-0.835-1.575-2.799-2.045-2.735-2.750-2.952-2.032-1.7702.711-2.847
), ArticleFig(id=1210147897232986392, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1210147888731132404, language=CN, label=Table 4, caption=

Content (mg·g-1) of ten components determined by quantitative analysis of multi-components by single marker method (QAMS) and external standard method (ESM). n = 3. RE (%) = (QAMS - ESM) / ESM × 100

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ComponentMethodS1S2S3S4S5S6S7S8S9S10S11S12S13S14S15S16S17S18S19S20
PCAESM0.4070.3290.4790.4830.5650.5620.3510.3980.3780.3810.4020.5550.5470.4450.4380.4770.4880.4310.4670.557
GAQAMS181.271287.284226.778219.914235.569228.139154.208190.281240.573258.270255.446215.742212.938295.892210.868217.165230.656206.914229.191217.284
ESM184.091287.350221.767223.912240.461233.833155.122193.599246.660254.182257.336219.053215.515301.985209.565217.125229.322207.548226.342222.842
RE (%)-1.532-0.0232.260-1.786-2.034-2.435-0.589-1.714-2.4681.608-0.734-1.511-1.196-2.0180.6220.0180.582-0.3051.258-2.494
SDSSQAMS3.0203.6513.6673.5762.6312.5123.4513.2316.0503.2243.2162.7002.5983.0353.3953.2403.5643.1583.3682.725
ESM2.9963.5993.5913.5682.6482.5693.3953.2446.1013.1363.1802.7762.5942.9983.2973.1843.4903.2403.3252.723
RE (%)0.8231.4542.1180.247-0.642-2.2331.655-0.412-0.8332.8181.139-2.7500.1501.2452.9431.7512.116-2.5231.2880.038
PCAEQAMS0.1280.1740.1550.1810.1100.1080.1760.1600.2830.1530.1490.1220.1210.1300.1420.1400.1480.1300.1340.127
ESM0.1290.1740.1540.1840.1130.1110.1750.1650.2890.1510.1500.1230.1210.1310.1380.1380.1460.1340.1380.128
RE (%)-0.412-0.1261.262-1.648-2.861-2.8260.678-2.936-2.2081.146-0.478-0.357-0.510-0.4682.2311.3501.422-2.648-2.710-0.258
VLQAMS72.72734.19979.17273.75366.40265.85256.23973.74833.31531.51235.52982.14583.17240.10272.48677.28384.46976.08279.75984.189
ESM73.16335.16578.49575.62465.14064.31957.05473.67934.23632.44336.13182.11684.47341.43474.55976.03982.65674.08077.52185.938
RE (%)-0.596-2.7460.862-2.4741.9382.384-1.4300.094-2.689-2.872-1.6660.035-1.5391.662-2.7811.6362.1932.7022.887-2.035
RAQAMS0.9850.9731.0951.1281.3001.1920.8320.9570.8260.9700.9740.8800.8180.9101.1061.0631.2051.0171.1560.854
ESM1.0121.0031.1261.1601.3321.2200.8560.9830.8510.9741.0020.9040.8420.9361.1311.0941.2310.9871.1850.876
RE (%)-2.657-2.967-2.749-2.746-2.374-2.275-2.850-2.608-2.985-0.451-2.810-2.716-2.905-2.791-2.160-2.826-2.0982.967-2.450-2.427
SABQAMS20.18117.54619.90417.92617.73516.92215.33517.08811.99016.86816.65714.63114.50318.54821.76020.53123.02821.24721.54614.558
ESM20.80317.76220.02018.44717.78617.43515.59517.47812.10516.77416.77614.98714.86618.69721.82420.89523.10121.88521.91814.919
RE (%)-2.989-1.215-0.584-2.827-0.285-2.944-1.666-2.233-0.9480.557-0.713-2.374-2.442-0.800-0.296-1.746-0.315-2.914-1.694-2.420
EGQAMS8.26310.5275.2763.2309.1139.81911.2787.86710.20511.73711.6528.7038.86812.1568.5538.8549.5008.6318.6778.773
ESM8.46010.5815.3983.3249.3149.90811.6218.0499.97812.09711.9188.8849.07712.5108.8079.1129.6168.8778.7808.986
RE (%)-2.330-0.507-2.249-2.825-2.151-0.899-2.953-2.2692.278-2.975-2.229-2.033-2.308-2.836-2.885-2.779-1.205-2.778-1.173-2.371
CTSQAMS1.4560.9361.3111.3811.1611.1811.3851.2591.4391.1441.5011.3971.7441.7501.3471.4761.5241.4181.6102.180
ESM1.4970.9311.3181.3951.1751.1921.3931.2921.4581.1491.5271.4051.7781.8001.3761.5181.5101.4501.5652.243
RE (%)-2.7600.492-0.560-1.004-1.216-0.920-0.560-2.561-1.322-0.363-1.670-0.612-1.929-2.747-2.137-2.7550.919-2.2052.872-2.795
TSTQAMS2.1681.1151.7251.7771.3131.3041.7801.6801.8801.5822.1111.7332.2692.2772.0112.0422.0592.0142.2513.017
ESM2.2301.1181.7541.8171.3131.3131.8011.7211.9191.5952.1441.7832.3172.3412.0682.1042.1022.0502.1913.105
RE (%)-2.758-0.311-1.667-2.176-0.738-0.738-1.1321.733-2.038-0.835-1.575-2.799-2.045-2.735-2.750-2.952-2.032-1.7702.711-2.847
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一测多评法同时测定冠心舒通胶囊中10个成分的含量
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龙凯花 1 , 刘峰 2, 3, 4 , 张红 1 , 杜霞 1 , 王春柳 1 , 刘洋 1 , 杨东花 5 , 李晔 1, *
药学学报 | 研究论文 2022,57(6): 1880-1886
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药学学报 | 研究论文 2022, 57(6): 1880-1886
一测多评法同时测定冠心舒通胶囊中10个成分的含量
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龙凯花1, 刘峰2, 3, 4, 张红1, 杜霞1, 王春柳1, 刘洋1, 杨东花5, 李晔1, *
作者信息
  • 1.陕西省中医药研究院, 陕西 西安 710003
  • 2.陕西步长制药有限公司, 陕西 西安 710075
  • 3.陕西国际商贸学院, 陕西 咸阳 712046
  • 4.陕西省中药绿色制造技术协同创新中心, 陕西 西安 710075
  • 5.青海省中医院, 青海 西宁 810000

通讯作者:

*李晔, Tel: 86-29-87251837, E-mail:
Simultaneous determination of 10 components in Guanxinshutong capsules by quantitative analysis of multi-components by single marker
Kai-hua LONG1, Feng LIU2, 3, 4, Hong ZHANG1, Xia DU1, Chun-liu WANG1, Yang LIU1, Dong-hua YANG5, Ye LI1, *
Affiliations
  • 1. Shaanxi Academy of Traditional Chinese Medicine, Xi'an 710003, China
  • 2. Shaanxi Buchang Pharmaceutical Limited Company, Xi'an 710075, China
  • 3. Shaanxi Institute of International Trade & Commerce, Xianyang 712046, China
  • 4. Collaborative Innovation Center of Green Manufacturing Technology for Traditional Chinese Medicine in Shaanxi Province, Xi'an 710075, China
  • 5. Qinghai Hospital of Traditional Chinese Medicine, Xining 810000, China
出版时间: 2022-06-12 doi: 10.16438/j.0513-4870.2022-0123
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摘要
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基于一测多评法建立高效液相色谱法同时测定冠心舒通胶囊(广枣、丹参、丁香、冰片、天竺黄) 中没食子酸、丹参素钠、原儿茶酸、原儿茶醛、香草醛、迷迭香酸、丹酚酸B、丁香酚、隐丹参酮、丹参酮ⅡA的含量。采用Agilent C18 (ZORBAX Extend-RP C18, 250 mm × 4.6 mm, 5 µm) 色谱柱, 以甲醇-0.4%甲酸水溶液为流动相梯度洗脱, 流速1.0 mL·min-1, 检测波长280 nm, 柱温35 ℃, 进样量5 µL。以原儿茶酸为内参物, 计算相对校正因子并进行耐用性考察, 通过一测多评法和外标法计算10个成分的含量。结果表明, 10种成分专属性、线性关系、精密度、重复性、稳定性均良好, 平均加样回收率98.20%~103.47%, RSD 1.26%~2.84%。计算其他9个成分的相对校正因子、含量(mg·g-1) 分别为没食子酸(0.759, 227.381)、丹参素钠(3.630, 3.283)、原儿茶醛(0.185, 0.150)、香草醛(0.532, 65.213)、迷迭香酸(4.240, 1.035)、丹酚酸B (3.245, 18.204)、丁香酚(1.729, 9.265)、隐丹参酮(0.691, 1.449)、丹参酮ⅡA (0.702, 1.939)。一测多评法所得结果与外标法接近, 相对误差在-3%~3%之间。表明该方法稳定可靠, 可用于冠心舒通胶囊中10个成分的含量测定。

冠心舒通胶囊  /  一测多评  /  相对校正因子  /  原儿茶酸  /  含量测定

This study establishes a quantitative analysis of multi-components by single marker (QAMS) method for the simultaneous determination of gallic acid, sodium danshensu, protocatechuic acid, protocatechuic aldehyde, vanillin, rosmarinic acid, salvianolic acid B, eugenol, cryptotanshinone and tanshinone IIA in Guanxinshutong capsules (Bambusae Concretio Silicea, Salvia miltiorrhiza, clove, borneol, Bambusae Concretio Silicea) by HPLC. Sample was loaded onto an Agilent C18 (ZORBAX Extend-RP C18, 250 mm × 4.6 mm, 5 µm) column and eluted with methanol-0.4% aqueous formic acid solution as a flow phase gradient, flow speed 1.0 mL·min-1, detection wavelength 280 nm, column temperature 35 ℃ and sample intake of 5 µL. Using protocatechuic acid as the internal reference, a relative correction factor was calculated and the durability was investigated, and the content of 10 components was calculated by QAMS and external standard method (ESM). The results show that the specificity, linear relationship, precision, repeatability, and stability of the 10 components were good. The average recovery was 98.20%-103.47% and RSD was 1.26%-2.84%. The relative positive factors and contents of the other nine components were calculated as gallic acid (0.759, 227.381), sodium tanshinol (3.630, 3.283), protocatechualdehyde (0.185, 0.150), vanillin (0.532, 65.213), rosmarinic acid (4.240, 1.035), salvianolic acid B (3.245, 18.204), eugenol (1.729, 9.265), cryptotanshinone (0.691, 1.449), and tanshinone ⅡA (0.702, 1.939). The results of QAMS were consistent with ESM analysis, and the relative error was between -3% and 3%. This method is stable and reliable, and can be used for the determination of 10 components in Guanxinshutong capsules.

Guanxinshutong capsule  /  quantitative analysis of multi-components by single marker  /  relative correction factor  /  protocatechuic acid  /  content determination
龙凯花, 刘峰, 张红, 杜霞, 王春柳, 刘洋, 杨东花, 李晔. 一测多评法同时测定冠心舒通胶囊中10个成分的含量. 药学学报, 2022 , 57 (6) : 1880 -1886 . DOI: 10.16438/j.0513-4870.2022-0123
Kai-hua LONG, Feng LIU, Hong ZHANG, Xia DU, Chun-liu WANG, Yang LIU, Dong-hua YANG, Ye LI. Simultaneous determination of 10 components in Guanxinshutong capsules by quantitative analysis of multi-components by single marker[J]. Acta Pharmaceutica Sinica, 2022 , 57 (6) : 1880 -1886 . DOI: 10.16438/j.0513-4870.2022-0123
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冠心舒通胶囊是由君药广枣、臣药丹参、佐药丁香和冰片、使药天竺黄五味构成, 用于胸痹心血瘀阻证, 临床用于治疗冠心病、心绞痛出现的胸痛、胸闷、心慌、气短等症[1]。目前, 关于冠心舒通胶囊的临床疗效与作用机制相关研究较多, 质量控制研究较少。2020版中国药典冠心舒通胶囊的含量测定方法分为气相色谱法与高效液相色谱法[2], 气相色谱法以龙脑、异龙脑、丁香酚为指标成分, 高效液相色谱法以丹参酮ⅡA、丹酚酸B为指标成分, 质量控制较为全面, 但存在以下3个问题: ①色谱条件较多(气相色谱法1个检测条件, 高效液相色谱法2个检测条件), 为含量测定带来不便; ②君药广枣具有行气活血, 养心, 安神的功效[3], 临床主要用于气滞血瘀, 胸痹作痛, 心悸气短, 心神不定等症, 对冠心舒通胶囊发挥疗效至关重要, 且广枣的量(480 g) 占整个处方量(840 g) 约57%, 所占比重较大, 但含量测定未纳入君药广枣的指标成分; ③含量测定部分共包含5个对照品, 成本较高。
一测多评法(quantitative analysis of multi-components by single marker, QAMS) 作为一种可实现多个成分同步含量测定的多指标质量控制分析方法, 已应用于各国药典, 在多种中药材、饮片及中药制剂的多成分含量测定中得到广泛应用[4, 5], 如丹参、重楼、保心宁片、脑心清片等。冠心舒通胶囊未见一测多评方面的研究报道, 因此本文以原儿茶酸为内标物, 建立一测多评法同时测定冠心舒通胶囊中没食子酸、丹参素钠、原儿茶酸、原儿茶醛、香草醛、迷迭香酸、丹酚酸B、丁香酚、隐丹参酮和丹参酮ⅡA 10种成分含量, 拟解决上述提到的色谱条件多、含量测定无君药广枣药效成分、成本高等问题。
材料与方法
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仪器与试剂   Agilent 1260型高效液相色谱仪(美国安捷伦公司); Waters 2695型高效液相色谱仪(美国Waters公司); 电子分析天平(型号BP211D, d = 0.01 mg, 德国赛多利斯天平有限公司); 超声波清洗器(型号: KQ-250DE型, 昆山市超声仪器有限公司); Agilent ZORBAX Extend-C18色谱柱(250 mm × 4.6 mm, 5 μm), Kromasil C18色谱柱(250 mm × 4.6 mm, 5 μm)、Diamonsil C18色谱柱(250 mm × 4.6 mm, 5 μm)。对照品丹参素钠(批号: 110855-201311, 纯度98.1%)、没食子酸(批号: 110831-200302, 纯度99.1%)、原儿茶醛(批号: 110810-201007, 纯度98.2%)、原儿茶酸(批号: 110809-201205, 纯度99.9%)、迷迭香酸(批号: 111871-201203, 纯度98.8%)、香草醛(批号: 100491-200901, 纯度100%, 供UV法测定)、丹参酮IIA (批号: 110766-200416, 纯度 > 99.8%)、丹酚酸B (批号: 111562-201313, 纯度97%)、丁香酚(批号: 110725-201213, 纯度99.7%)、隐丹参酮(批号: 110852-200806, 纯度 > 98%), 均购自中国食品药品检定研究院; 甲醇为色谱纯(美国Fisher试剂公司), 水为纯净水, 其他试剂均为分析纯。
实验材料   20批冠心舒通胶囊由陕西步长制药有限公司生产, 样品批号分别为120101 (S1)、120103 (S2)、120201 (S3)、120203 (S4)、120205 (S5)、120207 (S6)、120208 (S7)、120304 (S8)、120305 (S9)、120311 (S10)、120805 (S11)、120806 (S12)、120903 (S13)、120403 (S14)、120503 (S15)、130816 (S16)、131105 (S17)、131106 (S18)、131107 (S19)、131108 (S20)。
色谱条件    采用Agilent C18 (ZORBAX Extend RP C18, 250 mm × 4.6 mm, 5 µm) 色谱柱, 以甲醇(A)-0.4%甲酸水溶液(B) 为流动相, 梯度洗脱程序为0~15 min, 3%~28% A; 15~17 min, 28%~36% A; 17~40 min, 36%~38% A; 40~60 min, 38%~57% A; 60~80 min, 57%~75% A; 80~90 min, 75%~90% A; 90~100 min, 90%~100% A。流速1.0 mL·min-1, 检测波长280 nm, 柱温35 ℃, 进样量5 µL。
混合对照品溶液制备   精密称取没食子酸等10种对照品适量, 加甲醇溶液制成每1 mL分别含没食子酸7.575 μg、丹参素钠89.000 μg、原儿茶酸17.400 μg、原儿茶醛2.900 μg、香草醛2.178 μg、迷迭香酸73.814 μg、丹酚酸B 1.096 mg、丁香酚333.000 μg、隐丹参酮21.400 μg、丹参酮ⅡA 115.000 μg的溶液作为混合对照品溶液。
供试品溶液制备    精密称定冠心舒通胶囊内容物0.5 g, 置具塞锥形瓶中, 精密加入甲醇10 mL, 密塞, 称定重量, 超声(功率250 W, 频率40 kHz) 30 min, 放冷, 再称定重量, 用甲醇补足减失的重量, 摇匀, 滤过, 取续滤液, 即得。
专属性   取空白溶液、混合对照品溶液、供试品溶液分别进行测定, 比较色谱图。
线性关系   将混合对照品溶液分别进样2、4、6、8、10和12 μL, 以没食子酸、丹参素钠、原儿茶酸、原儿茶醛、香草醛、迷迭香酸、丹酚酸B、丁香酚、隐丹参酮、丹参酮IIA进样量(x, μg·mL-1) 与峰面积(Y) 作标准曲线, 计算回归方程。
精密度   精密吸取同一混合对照品溶液, 按色谱条件测定6次, 测定峰面积, 计算10种成分含量的RSD。
稳定性   取同一批供试品溶液, 于0、2、4、6、8、12和24 h按色谱条件进行测定, 记录峰面积, 计算10种成分含量的RSD。
重复性   取同一批供试品溶液, 按“供试品溶液制备”方法平行制备6份供试品溶液, 按色谱条件进行测定, 记录峰面积, 计算10种成分的含量RSD。
加样回收率   取6份冠心舒通胶囊内容物约0.25 g, 精密称定, 以1∶1的比例加入适量对照品溶液, 置具塞锥形瓶中, 精密加入甲醇10 mL, 密塞, 称定重量, 超声处理(功率250 W, 频率40 kHz) 30 min, 放冷, 再称定重量, 用甲醇补足减失的重量, 摇匀, 滤过, 取续滤液, 按色谱条件进行测定, 计算回收率。
相对校正因子的测定   相对校正因子(f) 计算公式为f = (As/Cs) / (Ai/Ci), 其中As、Cs分别为内参物对照品的峰面积和浓度; Ai、Ci分别为待测成分对照品的峰面积和浓度。分别进样4、6、8和10 μL, 按色谱条件项下测定各成分色谱峰峰面积, 以原儿茶酸为内参物, 计算不同进样量下其他9种成分的相对校正因子的平均值, 比较RSD值。
不同仪器、色谱柱对相对校正因子的影响   分别考察不同型号仪器(Agilent 1260型、Waters 2695型高效液相色谱仪) 和色谱柱(Agilent ZORBAX Extend-C18、Kromasil C18、Diamonsil C18, 规格为250 mm × 4.6 mm, 5 μm) 对相对校正因子的影响, 计算相对校正因子的平均值, 比较RSD值。
不同柱温对相对校正因子的影响   考察不同柱温(33、34、35、36和37 ℃) 对相对校正因子的影响, 计算相对校正因子的平均值, 比较RSD值。
不同流速对相对校正因子的影响   考察不同流速(0.8、0.9、1.0、1.1和1.2 mL·min-1) 对相对校正因子的影响, 计算相对校正因子的平均值, 比较RSD值。
待测组分色谱峰的定位   取混合对照品溶液, 按色谱条件分别测定不同型号仪器(Agilent 1260型、Waters 2695型高效液相色谱仪) 和色谱柱(Agilent ZORBAX Extend-C18、Kromasil C18、Diamonsil C18, 规格为250 mm × 4.6 mm, 5 μm) 各待测色谱峰的保留时间, 以原儿茶酸色谱峰保留时间为基准, 采用相对保留时间值对色谱峰进行定位, 并计算RSD值。
样品含量测定   取20批冠心舒通胶囊样品适量, 按供试品溶液制备方法制备样品溶液, 按色谱条件进行测定, 分别采用一测多评法和外标法(external standard method, ESM) 计算冠心舒通胶囊中没食子酸、丹参素钠、原儿茶酸、原儿茶醛、香草醛、迷迭香酸、丹酚酸B、丁香酚、隐丹参酮和丹参酮ⅡA的含量, 并用相对误差(RE) 进行评价。
结果
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1 方法学考察
1.1 系统适应性
10个成分与相邻色谱峰分离度均大于1.5, 色谱图见图 1, 专属性良好。
1.2 线性关系
各成分在各自范围内线性关系良好, 具体结果见表 1。
1.3 精密度
没食子酸、丹参素钠、原儿茶酸、原儿茶醛、香草醛、迷迭香酸、丹酚酸B、丁香酚、隐丹参酮和丹参酮ⅡA的RSD分别为0.266%、1.694%、1.354%、1.659%、1.333%、1.455%、1.580%、1.351%、1.807%和1.034%, 表明仪器精密度良好。
1.4 重复性
没食子酸、丹参素钠、原儿茶酸、原儿茶醛、香草醛、迷迭香酸、丹酚酸B、丁香酚、隐丹参酮和丹参酮ⅡA的RSD分别为2.877%、2.650%、2.727%、2.144%、1.199%、2.377%、1.906%、1.188%、2.805%和2.852%, 表明该方法重复性良好。
1.5 稳定性
没食子酸、丹参素钠、原儿茶酸、原儿茶醛、香草醛、迷迭香酸、丹酚酸B、丁香酚、隐丹参酮和丹参酮ⅡA的RSD分别为0.655%、0.924%、0.695%、0.749%、0.719%、1.601%、0.473%、0.492%、0.086%和0.998%, 表明溶液在24 h内稳定性良好。
1.6 加样回收率
没食子酸、丹参素钠、原儿茶酸、原儿茶醛、香草醛、迷迭香酸、丹酚酸B、丁香酚、隐丹参酮和丹参酮ⅡA的加样回收率分别为100.601%、99.149%、103.474%、103.362%、101.162%、98.609%、98.204%、101.981%、100.423%和102.083%, RSD分别为2.474%、2.463%、1.871%、1.845%、1.702%、1.763%、2.836%、1.253%、2.162%和2.823%, 表明方法准确度高。
2 一测多评法
2.1 相对校正因子的测定
以原儿茶酸为内参物, 没食子酸、丹参素钠、原儿茶醛、香草醛、迷迭香酸、丹酚酸B、丁香酚、隐丹参酮和丹参酮ⅡA的相对校正因子分别为0.759、3.630、0.185、0.532、4.240、3.245、1.729、0.691和0.702, RSD值均小于3%。
2.2 不同仪器、色谱柱对相对校正因子影响
不同高效色谱仪及色谱柱条件下, 计算各成分的相对校正因子如表 2所示, RSD值均小于2.5%, 表明不同的高效液相色谱仪和色谱柱对相对校正因子没有显著影响, 方法稳定性好。
2.3 不同柱温对相对校正因子的影响
不同柱温下计算相对校正因子, RSD值分别为1.311%、0.401%、2.184%、1.728%、0.931%、1.063%、1.252%、1.899%和2.416%, 表明各化合物相对校正因子在33~37 ℃的稳定性良好。
2.4 不同流速对相对校正因子的影响
不同流速下计算相对校正因子, RSD值分别为2.267%、1.426%、2.431%、2.006%、0.368%、0.664%、1.917%、1.503%和2.344%, 表明各成分相对校正因子在0.8~1.2 mL·min-1流速内稳定性良好。
2.5 待测组分色谱峰的定位
采用相对保留时间(tR) 法确定各待测组分的出峰时间[6, 7], 结果见表 3, RSD小于3%, 差异较小, 表明采用相对保留时间法对冠心舒通胶囊中10种有效成分进行峰定位具有可行性。
3 外标法和一测多评法含量测定比较
通过外标法测定冠心舒通胶囊中没食子酸、丹参素钠、原儿茶酸、原儿茶醛、香草醛、迷迭香酸、丹酚酸B、丁香酚、隐丹参酮和丹参酮ⅡA的平均含量分别为227.381、3.283、0.457、0.150、65.213、1.035、18.204、9.265、1.449和1.939 mg·g-1。采用一测多评法分别以原儿茶酸为内参物计算待测成分的含量, 与外标法测得的含量进行比较, 结果见表 4, 表明10种有效成分均能在20批样品中检测出来, 两种方法测得RE值在-3%~3%, 表明一测多评法可行, 结果准确度较高。
讨论
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本研究选择了活性明确、含量较高的代表成分作为冠心舒通胶囊质量控制的指标性成分进行一测多评法的含量测定, 主要包括了酚酸类没食子酸、原儿茶酸、迷迭香酸、丹酚酸B, 酚类丹参素钠、原儿茶醛、香草醛、丁香酚, 丹参酮类隐丹参酮和丹参酮ⅡA。其中没食子酸、丹参素钠、原儿茶酸、原儿茶醛、迷迭香酸和丹酚酸B具有改善心肌缺血、抑制动脉粥样硬化、抗血小板凝聚、缓解脑缺血再灌注、改善微小循环功能的药效作用[8-10], 香草醛可以通过血脑屏障, 通过减少体内和体外的氧化应激损伤而发挥神经保护作用[11], 丁香酚可通过调节下丘脑-垂体-肾上腺皮质和脑内单胺系统发挥抗应激作用在神经保护过程中发挥重要作用[12, 13], 隐丹参酮和丹参酮ⅡA是治疗心血管疾病的有效药物, 具有抗动脉粥样硬化、清除氧自由基、改善血流动力学、保护心肌及抑制心肌凋亡等作用[14]。因此, 所选指标成分均具有与冠心舒通胶囊中医理论、药理作用和主治功能相符的药理特性。
比较乙酸乙酯、50%甲醇、无水甲醇、50%乙醇、95%乙醇、无水乙醇和水等不同溶剂作为供试品提取溶剂时的峰面积的个数、峰面积的大小, 选择甲醇为最佳提取溶剂; 比较加热回流提取法与超声提取法用于供试品制备时的色谱图, 峰面积与个数差异不大, 考虑操作便捷选择超声提取法, 并对超声时间进行考察, 选择30 min; 先后考察了乙腈、甲醇为有机相, 磷酸、醋酸、甲酸水溶液为水相的洗脱结果, 甲醇-0.4%甲酸水溶液色谱峰能被较好的分离出来。
通过全波长扫描, 10种成分最大吸收在280 nm处较多, 其余吸收都在280 nm附近, 且色谱基线平稳、基线分离良好、灵敏度均较好, 可以满足定量分析要求, 因此选择280 nm作为检测波长。
一测多评法仅需1个价廉易得的有效成分, 即可实现多成分的同步测定[15]。原儿茶酸价廉易得, 是君药广枣发挥药理活性的重要物质基础[16]。2020版中国药典中使用原儿茶酸对照品的共9处, 其中北沙参药材、狗脊饮片、棕榈饮片、天王补心丸、急支糖浆、脑心清片、柿叶提取物以原儿茶酸为对照品进行薄层色谱鉴别, 狗脊饮片、刺五加浸膏以原儿茶酸为质量控制成分进行含量测定, 且为很多企业制剂、院内制剂的质控指标成分, 因此可采用原儿茶酸为内参物, 控制冠心舒通胶囊质量的同时, 也可用于其余药材、饮片、提取物、制剂的质量控制, 兼顾标品共享、节省成本的原则。
本课题组采用该测定方法, 构建心肌细胞缺氧/复氧(H/R) 模型, 测定10种成分对H/R心肌损伤作用, 结果表明除香草醛、没食子酸和原儿茶醛无保护作用, 其余均有明显的保护作用, 为冠心舒通胶囊治疗冠心病筛选出7种活性成分[17]。由于冠心舒通胶囊中佐药丁香和冰片均含有挥发油, 且龙脑、异龙脑是药典的质控成分, 因此下一步拟建立气相一测多评方法, 完善冠心舒通胶囊的质量控制体系。
作者贡献: 龙凯花进行了一测多评方法的建立、实验数据处理、撰写文章; 杜霞和刘洋进行了实验设计及文章修改等; 王春柳和杨东花对本文的方法建立提供帮助; 刘峰、张红、李晔从选题与设计、方法建立及文章修改提供了指导。
利益冲突: 无利益冲突。
基金
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  • 陕西省重点研发计划项目(2018ZDXM-SF-003)
  • 陕西省重点研发计划项目(2022ZDXM-SF-06)
  • 陕西省自然基础研究计划青年项目(2022JQ-971)
  • 陕西省中医药管理局—“秦药”研发重点实验室资助项目(2021-QYPT-001)
文献
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参考文献 引证文献
排序方式：
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2022年第57卷第6期
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doi: 10.16438/j.0513-4870.2022-0123
  • 接收时间：2022-01-26
  • 首发时间：2025-12-23
  • 出版时间：2022-06-12
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  • 收稿日期：2022-01-26
  • 修回日期：2022-03-08
基金
陕西省重点研发计划项目(2018ZDXM-SF-003)
陕西省重点研发计划项目(2022ZDXM-SF-06)
陕西省自然基础研究计划青年项目(2022JQ-971)
陕西省中医药管理局—“秦药”研发重点实验室资助项目(2021-QYPT-001)
作者信息
    1.陕西省中医药研究院, 陕西 西安 710003
    2.陕西步长制药有限公司, 陕西 西安 710075
    3.陕西国际商贸学院, 陕西 咸阳 712046
    4.陕西省中药绿色制造技术协同创新中心, 陕西 西安 710075
    5.青海省中医院, 青海 西宁 810000

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2种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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