首页 期刊 论文 学科刊群 资讯 加盟 关于
EN
image
Article(id=1200394153851998785, tenantId=1146029695717560320, journalId=1189982191388893191, issueId=1200394147019477416, articleNumber=null, orderNo=null, doi=10.16438/j.0513-4870.2023-1379, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=research-article, receivedDate=1702224000000, receivedDateStr=2023-12-11, revisedDate=1715356800000, revisedDateStr=2024-05-11, acceptedDate=null, acceptedDateStr=null, onlineDate=1764125868523, onlineDateStr=2025-11-26, pubDate=1720713600000, pubDateStr=2024-07-12, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1764125868523, onlineIssueDateStr=2025-11-26, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1764125868523, creator=13701087609, updateTime=1764125868523, updator=13701087609, issue=Issue{id=1200394147019477416, tenantId=1146029695717560320, journalId=1189982191388893191, year='2024', volume='59', issue='7', pageStart='1897', pageEnd='2182', issueExtLink='null', onlineDate='null', pubDate='null', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1764125866894, creator=13701087609, updateTime=1764225115484, updator=13701087609, preIssue=null, nextIssue=null, ext={EN=IssueExt(id=1200810425920115296, tenantId=1146029695717560320, journalId=1189982191388893191, issueId=1200394147019477416, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1200810425920115297, tenantId=1146029695717560320, journalId=1189982191388893191, issueId=1200394147019477416, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null}, startPage=2108, endPage=2116, ext={EN=ArticleExt(id=1200394154263040594, articleId=1200394153851998785, tenantId=1146029695717560320, journalId=1189982191388893191, language=EN, title=Study on the characteristic branch sites of oligosaccharides of Astragalus polysaccharide APS-Ⅱ enzymolysis based on high resolution mass spectrometry, columnId=1190335348761793317, journalTitle=Acta Pharmaceutica Sinica, columnName=Original Articles, runingTitle=null, highlight=null, articleAbstract=

Astragalus polysaccharides are the most immunoregulatory active and abundant substances in Astragalus, with anti-tumor, anti-viral, and immune-promoting biological activities. They have been widely used in clinical practice. Previous studies have found that Astragalus polysaccharides are mainly composed of two different polysaccharides, APS-Ⅰ (> 2 000 kDa) and APS-Ⅱ (10 kDa), with APS-Ⅱ (10 kDa) being the most active component of Astragalus polysaccharides. We used α-1, 4-glucan endo-hydrolysis enzyme to degrade APS-Ⅱ into oligosaccharides, and screened the immune activity of oligosaccharides in vitro. We found that the overall immune activity of 2-9 oligosaccharides was low, while the immune activity of 10-14 oligosaccharides was strong, and the activity was better than that of untreated APS-Ⅱ. To investigate the key structural features of APS-Ⅱ oligosaccharides that play a role in immune activity, we used MALDI-TOF-MS biochemical mass spectrometry and high-resolution mass spectrometry instrument ESI-Q Exactive-MS to analyze the APS enzymatic oligosaccharides. By comparing, we found that 10-14 oligosaccharides contain 1→4 and 1→6 branched structures with coexisting linkages, suggesting that 1→4 and 1→6 linkages in branched structures are key structural features that play a role in the immune activity of APS-Ⅱ, laying a theoretical foundation for the structure-activity relationship of Astragalus polysaccharides and oligosaccharides.

, correspAuthors=Ke LI, authorNote=null, correspAuthorsNote=null, copyrightStatement=Copyright ©2024 Acta Pharmaceutica Sinica. All rights reserved., copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, authorCompany=null, fund=null, authors=null, authorsList=Yu-chong LIU, Hu-feng LI, Ke LI, Xue-mei QIN, Yu-guang DU, Zhen-yu LI), CN=ArticleExt(id=1200394157568152293, articleId=1200394153851998785, tenantId=1146029695717560320, journalId=1189982191388893191, language=CN, title=基于高分辨质谱技术的黄芪多糖APS-Ⅱ酶解活性寡糖特征分支位点的研究, columnId=1190335348896011050, journalTitle=药学学报, columnName=研究论文, runingTitle=null, highlight=null, articleAbstract=

黄芪多糖是黄芪中免疫调节活性最强, 含量最为丰富的物质, 具有抗肿瘤、抗病毒、免疫促进等多种生物活性, 临床应用广泛。前期研究发现黄芪多糖主要由2种不同相对分子质量多糖APS-Ⅰ (> 2 000 kDa)、APS-Ⅱ (10 kDa) 组成, APS-Ⅱ (10 kDa) 为黄芪多糖中活性最强组分, 并采用α-1, 4-葡聚糖内切酶将APS-Ⅱ降解为寡糖, 通过体外免疫活性筛选发现2~9糖整体免疫活性较低, 而10~14糖具有较强的免疫活性, 且活性优于未降解的APS-Ⅱ。为了探究APS-Ⅱ降解寡糖发挥免疫活性的关键结构, 本文采用MALDI-TOF-MS生物质谱以及高分辨质谱仪器ESI-Q Exactive-MS对APS酶解寡糖进行解析, 通过对比发现10~14糖中存在1→4和1→6两种连接方式共存的支链结构, 推测1→4和1→6两种连接方式共存的支链结构为APS-Ⅱ发挥免疫活性的关键结构, 为黄芪多糖和寡糖的构效关系研究奠定理论基础。

, correspAuthors=李科, authorNote=null, correspAuthorsNote=
*李科, Tel / Fax: 86-351-7019297, E-mail:
, copyrightStatement=版权所有©《药学学报》编辑部2024, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=9R4W/ttDa5rsn+mJ6vyX4g==, magXml=GyPdntgqcW6e5Ejz8iiQTQ==, pdfUrl=null, pdf=+rcwrsGFO+tEcdGlxuUqhw==, pdfFileSize=1273509, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=K8f3tceFfi7iE/Kc82lV7g==, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=OazkPzURd2Vo6eKMl0G61g==, mapNumber=null, authorCompany=null, fund=null, authors=null, authorsList=刘宇冲, 李虎峰, 李科, 秦雪梅, 杜昱光, 李震宇)}, authors=[Author(id=1200470889746789281, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, orderNo=0, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1200470889897784232, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, authorId=1200470889746789281, language=EN, stringName=Yu-chong LIU, firstName=Yu-chong, middleName=null, lastName=LIU, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, 2, 3, address=1. Modern Research Center for Traditional Chinese Medicine of Shanxi University, Taiyuan 030006, China
2. Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education of Shanxi University, Taiyuan 030006, China
3. Key Laboratory of Effective Substances Research and Utilization in TCM of Shanxi Province, Taiyuan 030006, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1200470889994253228, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, authorId=1200470889746789281, language=CN, stringName=刘宇冲, firstName=宇冲, middleName=null, lastName=刘, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, 2, 3, address=1.山西大学中医药现代研究中心, 山西 太原 030006
2.山西大学化学, 生物学与分子工程教育部重点实验室, 山西 太原 030006
3.地产中药功效物质研究与利用山西省重点实验室, 山西 太原 030006, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1200470889243472774, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, xref=null, ext=[AuthorCompanyExt(id=1200470889256055687, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, companyId=1200470889243472774, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1. Modern Research Center for Traditional Chinese Medicine of Shanxi University, Taiyuan 030006, China), AuthorCompanyExt(id=1200470889264444296, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, companyId=1200470889243472774, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1.山西大学中医药现代研究中心, 山西 太原 030006)]), AuthorCompany(id=1200470889352524681, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, xref=null, ext=[AuthorCompanyExt(id=1200470889360913290, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, companyId=1200470889352524681, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2. Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education of Shanxi University, Taiyuan 030006, China), AuthorCompanyExt(id=1200470889373496203, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, companyId=1200470889352524681, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2.山西大学化学, 生物学与分子工程教育部重点实验室, 山西 太原 030006)]), AuthorCompany(id=1200470889490936721, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, xref=null, ext=[AuthorCompanyExt(id=1200470889499325330, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, companyId=1200470889490936721, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=3. Key Laboratory of Effective Substances Research and Utilization in TCM of Shanxi Province, Taiyuan 030006, China), AuthorCompanyExt(id=1200470889507713939, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, companyId=1200470889490936721, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=3.地产中药功效物质研究与利用山西省重点实验室, 山西 太原 030006)])]), Author(id=1200470890078139312, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, orderNo=1, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1200470890229134262, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, authorId=1200470890078139312, language=EN, stringName=Hu-feng LI, firstName=Hu-feng, middleName=null, lastName=LI, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, 2, 3, address=1. Modern Research Center for Traditional Chinese Medicine of Shanxi University, Taiyuan 030006, China
2. Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education of Shanxi University, Taiyuan 030006, China
3. Key Laboratory of Effective Substances Research and Utilization in TCM of Shanxi Province, Taiyuan 030006, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1200470890325603258, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, authorId=1200470890078139312, language=CN, stringName=李虎峰, firstName=虎峰, middleName=null, lastName=李, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, 2, 3, address=1.山西大学中医药现代研究中心, 山西 太原 030006
2.山西大学化学, 生物学与分子工程教育部重点实验室, 山西 太原 030006
3.地产中药功效物质研究与利用山西省重点实验室, 山西 太原 030006, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1200470889243472774, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, xref=null, ext=[AuthorCompanyExt(id=1200470889256055687, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, companyId=1200470889243472774, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1. Modern Research Center for Traditional Chinese Medicine of Shanxi University, Taiyuan 030006, China), AuthorCompanyExt(id=1200470889264444296, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, companyId=1200470889243472774, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1.山西大学中医药现代研究中心, 山西 太原 030006)]), AuthorCompany(id=1200470889352524681, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, xref=null, ext=[AuthorCompanyExt(id=1200470889360913290, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, companyId=1200470889352524681, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2. Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education of Shanxi University, Taiyuan 030006, China), AuthorCompanyExt(id=1200470889373496203, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, companyId=1200470889352524681, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2.山西大学化学, 生物学与分子工程教育部重点实验室, 山西 太原 030006)]), AuthorCompany(id=1200470889490936721, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, xref=null, ext=[AuthorCompanyExt(id=1200470889499325330, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, companyId=1200470889490936721, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=3. Key Laboratory of Effective Substances Research and Utilization in TCM of Shanxi Province, Taiyuan 030006, China), AuthorCompanyExt(id=1200470889507713939, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, companyId=1200470889490936721, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=3.地产中药功效物质研究与利用山西省重点实验室, 山西 太原 030006)])]), Author(id=1200470890422072256, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, orderNo=2, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=like@sxu.edu.cn, emailSecond=null, emailThird=null, correspondingAuthor=1, authorType=1, ext={EN=AuthorExt(id=1200470890556289989, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, authorId=1200470890422072256, language=EN, stringName=Ke LI, firstName=Ke, middleName=null, lastName=LI, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, 2, 3, 4, *, address=1. Modern Research Center for Traditional Chinese Medicine of Shanxi University, Taiyuan 030006, China
2. Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education of Shanxi University, Taiyuan 030006, China
3. Key Laboratory of Effective Substances Research and Utilization in TCM of Shanxi Province, Taiyuan 030006, China
4. Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1200470890711479240, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, authorId=1200470890422072256, language=CN, stringName=李科, firstName=科, middleName=null, lastName=李, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, 2, 3, 4, *, address=1.山西大学中医药现代研究中心, 山西 太原 030006
2.山西大学化学, 生物学与分子工程教育部重点实验室, 山西 太原 030006
3.地产中药功效物质研究与利用山西省重点实验室, 山西 太原 030006
4.中国科学院过程工程研究所, 北京 100190, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1200470889243472774, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, xref=null, ext=[AuthorCompanyExt(id=1200470889256055687, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, companyId=1200470889243472774, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1. Modern Research Center for Traditional Chinese Medicine of Shanxi University, Taiyuan 030006, China), AuthorCompanyExt(id=1200470889264444296, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, companyId=1200470889243472774, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1.山西大学中医药现代研究中心, 山西 太原 030006)]), AuthorCompany(id=1200470889352524681, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, xref=null, ext=[AuthorCompanyExt(id=1200470889360913290, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, companyId=1200470889352524681, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2. Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education of Shanxi University, Taiyuan 030006, China), AuthorCompanyExt(id=1200470889373496203, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, companyId=1200470889352524681, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2.山西大学化学, 生物学与分子工程教育部重点实验室, 山西 太原 030006)]), AuthorCompany(id=1200470889490936721, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, xref=null, ext=[AuthorCompanyExt(id=1200470889499325330, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, companyId=1200470889490936721, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=3. Key Laboratory of Effective Substances Research and Utilization in TCM of Shanxi Province, Taiyuan 030006, China), AuthorCompanyExt(id=1200470889507713939, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, companyId=1200470889490936721, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=3.地产中药功效物质研究与利用山西省重点实验室, 山西 太原 030006)]), AuthorCompany(id=1200470889637737369, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, xref=null, ext=[AuthorCompanyExt(id=1200470889646125978, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, companyId=1200470889637737369, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=4. Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China), AuthorCompanyExt(id=1200470889654514588, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, companyId=1200470889637737369, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=4.中国科学院过程工程研究所, 北京 100190)])]), Author(id=1200470890921194447, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, orderNo=3, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1200470891130909659, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, authorId=1200470890921194447, language=EN, stringName=Xue-mei QIN, firstName=Xue-mei, middleName=null, lastName=QIN, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, 2, 3, address=1. Modern Research Center for Traditional Chinese Medicine of Shanxi University, Taiyuan 030006, China
2. Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education of Shanxi University, Taiyuan 030006, China
3. Key Laboratory of Effective Substances Research and Utilization in TCM of Shanxi Province, Taiyuan 030006, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1200470891273516001, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, authorId=1200470890921194447, language=CN, stringName=秦雪梅, firstName=雪梅, middleName=null, lastName=秦, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, 2, 3, address=1.山西大学中医药现代研究中心, 山西 太原 030006
2.山西大学化学, 生物学与分子工程教育部重点实验室, 山西 太原 030006
3.地产中药功效物质研究与利用山西省重点实验室, 山西 太原 030006, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1200470889243472774, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, xref=null, ext=[AuthorCompanyExt(id=1200470889256055687, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, companyId=1200470889243472774, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1. Modern Research Center for Traditional Chinese Medicine of Shanxi University, Taiyuan 030006, China), AuthorCompanyExt(id=1200470889264444296, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, companyId=1200470889243472774, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1.山西大学中医药现代研究中心, 山西 太原 030006)]), AuthorCompany(id=1200470889352524681, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, xref=null, ext=[AuthorCompanyExt(id=1200470889360913290, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, companyId=1200470889352524681, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2. Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education of Shanxi University, Taiyuan 030006, China), AuthorCompanyExt(id=1200470889373496203, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, companyId=1200470889352524681, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2.山西大学化学, 生物学与分子工程教育部重点实验室, 山西 太原 030006)]), AuthorCompany(id=1200470889490936721, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, xref=null, ext=[AuthorCompanyExt(id=1200470889499325330, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, companyId=1200470889490936721, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=3. Key Laboratory of Effective Substances Research and Utilization in TCM of Shanxi Province, Taiyuan 030006, China), AuthorCompanyExt(id=1200470889507713939, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, companyId=1200470889490936721, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=3.地产中药功效物质研究与利用山西省重点实验室, 山西 太原 030006)])]), Author(id=1200470891416122345, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, orderNo=4, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1200470891722306557, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, authorId=1200470891416122345, language=EN, stringName=Yu-guang DU, firstName=Yu-guang, middleName=null, lastName=DU, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=4, address=4. Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1200470891827163140, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, authorId=1200470891416122345, language=CN, stringName=杜昱光, firstName=昱光, middleName=null, lastName=杜, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=4, address=4.中国科学院过程工程研究所, 北京 100190, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1200470889637737369, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, xref=null, ext=[AuthorCompanyExt(id=1200470889646125978, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, companyId=1200470889637737369, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=4. Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China), AuthorCompanyExt(id=1200470889654514588, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, companyId=1200470889637737369, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=4.中国科学院过程工程研究所, 北京 100190)])]), Author(id=1200470891957186571, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, orderNo=5, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1200470892145930264, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, authorId=1200470891957186571, language=EN, stringName=Zhen-yu LI, firstName=Zhen-yu, middleName=null, lastName=LI, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, 2, 3, address=1. Modern Research Center for Traditional Chinese Medicine of Shanxi University, Taiyuan 030006, China
2. Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education of Shanxi University, Taiyuan 030006, China
3. Key Laboratory of Effective Substances Research and Utilization in TCM of Shanxi Province, Taiyuan 030006, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1200470892259176478, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, authorId=1200470891957186571, language=CN, stringName=李震宇, firstName=震宇, middleName=null, lastName=李, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, 2, 3, address=1.山西大学中医药现代研究中心, 山西 太原 030006
2.山西大学化学, 生物学与分子工程教育部重点实验室, 山西 太原 030006
3.地产中药功效物质研究与利用山西省重点实验室, 山西 太原 030006, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1200470889243472774, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, xref=null, ext=[AuthorCompanyExt(id=1200470889256055687, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, companyId=1200470889243472774, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1. Modern Research Center for Traditional Chinese Medicine of Shanxi University, Taiyuan 030006, China), AuthorCompanyExt(id=1200470889264444296, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, companyId=1200470889243472774, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1.山西大学中医药现代研究中心, 山西 太原 030006)]), AuthorCompany(id=1200470889352524681, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, xref=null, ext=[AuthorCompanyExt(id=1200470889360913290, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, companyId=1200470889352524681, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2. Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education of Shanxi University, Taiyuan 030006, China), AuthorCompanyExt(id=1200470889373496203, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, companyId=1200470889352524681, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2.山西大学化学, 生物学与分子工程教育部重点实验室, 山西 太原 030006)]), AuthorCompany(id=1200470889490936721, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, xref=null, ext=[AuthorCompanyExt(id=1200470889499325330, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, companyId=1200470889490936721, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=3. Key Laboratory of Effective Substances Research and Utilization in TCM of Shanxi Province, Taiyuan 030006, China), AuthorCompanyExt(id=1200470889507713939, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, companyId=1200470889490936721, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=3.地产中药功效物质研究与利用山西省重点实验室, 山西 太原 030006)])])], keywords=[Keyword(id=1200470892468891695, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, language=EN, orderNo=1, keyword=APS-Ⅱ), Keyword(id=1200470892611498040, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, language=EN, orderNo=2, keyword=enzymolysis), Keyword(id=1200470892733132865, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, language=EN, orderNo=3, keyword=connection mode), Keyword(id=1200470892846379080, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, language=EN, orderNo=4, keyword=structural analysis), Keyword(id=1200470892938653777, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, language=EN, orderNo=5, keyword=high resolution mass spectrometry), Keyword(id=1200470893110620251, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, language=CN, orderNo=1, keyword=APS-Ⅱ), Keyword(id=1200470893211283556, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, language=CN, orderNo=2, keyword=酶解), Keyword(id=1200470893282586730, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, language=CN, orderNo=3, keyword=连接方式), Keyword(id=1200470893383250034, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, language=CN, orderNo=4, keyword=结构解析), Keyword(id=1200470893525856378, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, language=CN, orderNo=5, keyword=高分辨质谱)], refs=[Reference(id=1200470896537366892, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[1], rfOrder=0, authorNames=null, journalName=null, refType=null, unstructuredReference=Li Q, Hu J, Gao B, et al. Advances on immunoregulation effect of Astragalus polysaccharides [J]. Chin J Exp Tradit Med Form (中国实验方剂学杂志), 2017, 23: 199-206., articleTitle=null, refAbstract=null), Reference(id=1200470896650613109, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[2], rfOrder=1, authorNames=null, journalName=null, refType=null, unstructuredReference=Morten L, Anette W, Rune L, et al. Enhancement of human adaptive immune responses by administration of a high-molecular-weight polysaccharide extract from the cyanobacterium Arthrospira platensis [J]. J Med Food, 2008, 11: 313-322., articleTitle=null, refAbstract=null), Reference(id=1200470896751276412, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[3], rfOrder=2, authorNames=null, journalName=null, refType=null, unstructuredReference=Jiang C, Tang C, Qiang Y, et al. Immunoregulation effect of Astragalus polysaccharides [J]. Food Sci (食品科学), 2020, 34: 327-332., articleTitle=null, refAbstract=null), Reference(id=1200470896898077062, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[4], rfOrder=3, authorNames=null, journalName=null, refType=null, unstructuredReference=Li K, Cui LJ, Cao YX, et al. UHPLC Q-Exactive MS-based serum metabolomics to explore the effect mechanisms of immunological activity of Astragalus polysaccharides with different molecular weights [J]. Front Pharmacol, 2020, 11: 595692., articleTitle=null, refAbstract=null), Reference(id=1200470897036489101, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[5], rfOrder=4, authorNames=null, journalName=null, refType=null, unstructuredReference=Li K, Cao YX, Jiao SM, et al. Structural characterization and immune activity screening of polysaccharides with different molecular weights from Astragali Radix [J]. Front Pharmacol, 2020, 11: 582091., articleTitle=null, refAbstract=null), Reference(id=1200470897141346711, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[6], rfOrder=5, authorNames=null, journalName=null, refType=null, unstructuredReference=Li K, Li XQ, Li GX, et al. Radix by enzymolysis of endo α-1, 4-glucanas relationship between the structure and immune activity of components from the active polysaccharides APS-Ⅱ of Astragalie [J]. Front Pharmacol, 2022, 13: 839635., articleTitle=null, refAbstract=null), Reference(id=1200470897330090404, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[7], rfOrder=6, authorNames=null, journalName=null, refType=null, unstructuredReference=Li Q. Preparation of Apple Oligosaccharides, Structural Identification and Antitumor Activity (苹果多糖的制备、结构鉴定及抗肿瘤活性) [D]. Xi'an: Fourth Military Medical University, 2013., articleTitle=null, refAbstract=null), Reference(id=1200470897464308140, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[8], rfOrder=7, authorNames=null, journalName=null, refType=null, unstructuredReference=Miu YQ, Gu GP, Wu GR, et al. Research progress on hydrolysis of plant polysaccharides and their products [J]. Chin Wild Plant Resour (中国野生植物资源), 2005, 02: 4-7., articleTitle=null, refAbstract=null), Reference(id=1200470897585942964, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[9], rfOrder=8, authorNames=null, journalName=null, refType=null, unstructuredReference=Li D, Song Y, Wu M, et al. Review on the hydrolysis of plant polysaccharides and the structure of hydrolysate [J]. Food Fermn Ind (食品与发酵工业), 2013, 39: 165-170., articleTitle=null, refAbstract=null), Reference(id=1200470897808241088, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[10], rfOrder=9, authorNames=null, journalName=null, refType=null, unstructuredReference=Li P, Li WY, Cao W, et al. EI candy and its derivative molecular ion ESI-MSN broken behavior preliminary research [J]. Northwest Pharm J (西北药学杂志), 2015, 30: 433-436., articleTitle=null, refAbstract=null), Reference(id=1200470897971818953, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[11], rfOrder=10, authorNames=null, journalName=null, refType=null, unstructuredReference=Li K, Cui LJ, Li XX, et al. Establishment of enzymatic hydrolysis method of active Astragalus polysaccharides APS-Ⅱ and study on the immune activity of oligosaccharides after its degradation [J]. Acta Pharm Sin (药学学报), 2021, 56: 1936-1944., articleTitle=null, refAbstract=null), Reference(id=1200470898101842385, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[12], rfOrder=11, authorNames=null, journalName=null, refType=null, unstructuredReference=Wang S, Chen C, Guan M, et al. Terminal epitope-dependent branch preference of siglecs toward N-glycans [J]. Front Mol Biosci, 2021, 8: 645999., articleTitle=null, refAbstract=null), Reference(id=1200470898282197473, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[13], rfOrder=12, authorNames=null, journalName=null, refType=null, unstructuredReference=Cui LJ. Astragalus Active Polysaccharide APS-Ⅱ. Immune Regulation Mechanism and Structure and Activity of Enzymatic Hydrolyzable Oligosaccharide Fragments (黄芪活性多糖APS-Ⅱ免疫调控机制及其酶解寡糖片段的结构和活性研究) [D]. Taiyuan: Shanxi University, 2021., articleTitle=null, refAbstract=null), Reference(id=1200470898445775334, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[14], rfOrder=13, authorNames=null, journalName=null, refType=null, unstructuredReference=Wang Z. Preliminary Studies on Derivatived Oligosaccharide Molecular Ions Fragmentation Pattern in ESI-MSn Analysis (ESI-MSn分析中衍生寡糖分子离子裂解模式的初步研究) [D]. Xi'an: Northwest University, 2011., articleTitle=null, refAbstract=null), Reference(id=1200470898596770288, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[15], rfOrder=14, authorNames=null, journalName=null, refType=null, unstructuredReference=He BL, Zheng QW, Guo LQ, et al. Structural characterization and immune-enhancing activity of a novel high-molecular-weight polysaccharide from Cordyceps militaris [J]. Int J Biol Macromol, 2020, 145: 11-20., articleTitle=null, refAbstract=null)], funds=[Fund(id=1200470895564288281, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, awardId=81872962, language=CN, fundingSource=国家自然科学基金资助项目(81872962), fundOrder=null, country=null), Fund(id=1200470895669145890, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, awardId=2019M650851, language=CN, fundingSource=国家博士后科学基金资助项目(2019M650851), fundOrder=null, country=null), Fund(id=1200470895782392111, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, awardId=2019YFC1710800, language=CN, fundingSource=国家重点研发计划(2019YFC1710800), fundOrder=null, country=null), Fund(id=1200470895895638329, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, awardId=201603D311101, language=CN, fundingSource=山西省重点研发计划重点项目(201603D311101), fundOrder=null, country=null), Fund(id=1200470896017273155, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, awardId=201605D211030, language=CN, fundingSource=山西省优秀人才科技创新项目(201605D211030), fundOrder=null, country=null), Fund(id=1200470896109547857, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, awardId=201705D211020, language=CN, fundingSource=山西省优秀人才科技创新项目(201705D211020), fundOrder=null, country=null), Fund(id=1200470896218599773, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, awardId=null, language=CN, fundingSource=山西省科技创新人才团队专项基金, fundOrder=null, country=null)], companyList=[AuthorCompany(id=1200470889243472774, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, xref=null, ext=[AuthorCompanyExt(id=1200470889256055687, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, companyId=1200470889243472774, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1. Modern Research Center for Traditional Chinese Medicine of Shanxi University, Taiyuan 030006, China), AuthorCompanyExt(id=1200470889264444296, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, companyId=1200470889243472774, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1.山西大学中医药现代研究中心, 山西 太原 030006)]), AuthorCompany(id=1200470889352524681, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, xref=null, ext=[AuthorCompanyExt(id=1200470889360913290, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, companyId=1200470889352524681, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2. Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education of Shanxi University, Taiyuan 030006, China), AuthorCompanyExt(id=1200470889373496203, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, companyId=1200470889352524681, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2.山西大学化学, 生物学与分子工程教育部重点实验室, 山西 太原 030006)]), AuthorCompany(id=1200470889490936721, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, xref=null, ext=[AuthorCompanyExt(id=1200470889499325330, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, companyId=1200470889490936721, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=3. Key Laboratory of Effective Substances Research and Utilization in TCM of Shanxi Province, Taiyuan 030006, China), AuthorCompanyExt(id=1200470889507713939, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, companyId=1200470889490936721, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=3.地产中药功效物质研究与利用山西省重点实验室, 山西 太原 030006)]), AuthorCompany(id=1200470889637737369, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, xref=null, ext=[AuthorCompanyExt(id=1200470889646125978, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, companyId=1200470889637737369, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=4. Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China), AuthorCompanyExt(id=1200470889654514588, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, companyId=1200470889637737369, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=4.中国科学院过程工程研究所, 北京 100190)])], figs=[ArticleFig(id=1200470893798486164, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, language=EN, label=null, caption=null, figureFileSmall=RXhJ52jId6yZePFbaDjtzw==, figureFileBig=Znw9YQHK8Ex9XcBZMTgX4w==, tableContent=null), ArticleFig(id=1200470894058533026, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, language=CN, label=Figure 1, caption= MALDI-TOF-MS diagram of APS-Ⅱ enzymatic oligosaccharide. APS: <i>Astragalus</i> polysaccharide , figureFileSmall=RXhJ52jId6yZePFbaDjtzw==, figureFileBig=Znw9YQHK8Ex9XcBZMTgX4w==, tableContent=null), ArticleFig(id=1200470894247276721, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, language=EN, label=null, caption=null, figureFileSmall=s6O9GD1NXNJ5gw5SI/ro/g==, figureFileBig=lpJ1GatSzIBCMU6AJws1dQ==, tableContent=null), ArticleFig(id=1200470894364717239, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, language=CN, label=Figure 2, caption= APS-Ⅱ enzymatic oligosaccharide ESI-Q Exactive-MS positive ion mode total ion current , figureFileSmall=s6O9GD1NXNJ5gw5SI/ro/g==, figureFileBig=lpJ1GatSzIBCMU6AJws1dQ==, tableContent=null), ArticleFig(id=1200470894507323582, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, language=EN, label=null, caption=null, figureFileSmall=iJdBPzBp7Uh42nscNbIPZQ==, figureFileBig=3cXqaePspBXXcuFYcHZ7tA==, tableContent=null), ArticleFig(id=1200470894607986891, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, language=CN, label=Figure 3, caption= MS/MS spectrum of APS-Ⅱ enzymatic hydrolysis of decasaccharide , figureFileSmall=iJdBPzBp7Uh42nscNbIPZQ==, figureFileBig=3cXqaePspBXXcuFYcHZ7tA==, tableContent=null), ArticleFig(id=1200470894725427414, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, language=EN, label=null, caption=null, figureFileSmall=vW0QKQ49p7hgYrGIwkvKiA==, figureFileBig=YqrT/p/zaezZYN6lsaKcyQ==, tableContent=null), ArticleFig(id=1200470894863839455, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, language=CN, label=Figure 4, caption= MS/MS spectrum of APS-Ⅱ enzymatic hydrolysis of tetradecacose , figureFileSmall=vW0QKQ49p7hgYrGIwkvKiA==, figureFileBig=YqrT/p/zaezZYN6lsaKcyQ==, tableContent=null), ArticleFig(id=1200470894964502760, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, language=EN, label=null, caption=null, figureFileSmall=null, figureFileBig=null, tableContent=
DPtR/minMolecular formulaTheoretical value of relative molecular massFragment ions/m/z (M+Na+)
22.17C12H22O11342.296 5203.053 4, 219.059 1, 269.657 9, 305.083 8, 347.095 4, 365.105 7
33.04C18H32O16504.437 1185.040 6, 203.053 3, 347.095 1, 365.106 2, 425.203 7, 467.140 2, 527.158 9
43.87C24H42O21666.577 7185.042 8, 203.053 2, 268.599 6, 347.053 2, 365.106 0, 467.135 7, 509.150 5, 527.160 1, 689.212 2
54.90C30H52O26828.718 3185.042 8, 229.494 2, 268.599 6, 323.433 1, 347.094 5, 365.105 7, 467.137 4, 509.148 2, 527.158 1, 671.202 5, 689.211 9, 851.264 1
66.25C36H62O31990.858 9185.043 2, 203.053 7, 263.064 4, 325.113 2, 347.095 3, 365.105 1, 467.141 7, 483.514 1, 509.148 2, 527.158 5, 671.201 9, 689.210 6, 791.246 3, 833.256 1, 851.265 3, 839.280 1, 953.312 5, 1 013.317 2
77.88C42H72O361 152.999 5185.028 6, 203.039 0, 227.040 8, 245.051 2, 275.062 3, 287.060 4, 305.071 8, 365.105 6, 407.105 1, 467.136 3, 509.147 3, 527.157 7, 569.155 2, 629.185 8, 671.199 3, 689.211 4, 731.210 1, 791.240 4, 833.254 2, 851.263 4, 893.363 3, 908.774 5, 953.281 3, 995.288 7, 1 013.317 4, 1 055.318 3, 1 072.371 2, 1 115.331 3, 1 175.370 2
89.14C48H82O411 315.140 1185.042 6, 219.018 1, 245.051 6, 263.060 2, 272.065 7, 325.113 8, 347.095 1, 365.103 8, 407.106 5, 467.137 3, 509.147 8, 527.158 6, 569.131 5, 629.189 2, 671.200 6, 689.211 6, 731.213 6, 791.251 8, 833.251 9, 851.264 7, 893.286 1, 953.286 1, 1 013.317 3, 1 055.316 3, 1 115.331 2, 1 175.370 7, 1 217.366 7, 1 277.389 5, 1 337.425 3
910.51C54H92O461 477.280 7185.043 1, 191.039 2, 203.052 6, 325.113 7, 347.095 2, 365.106 4, 407.111 2, 467.140 5, 509.148 2, 527.158 8, 569.169 9, 629.193 7, 689.211 9 731.317 2, 759.219 7, 791.238 2, 833.253 1, 851.264 5, 953.293 8, 995.304 8, 1 013.317 0, 1 055.317 2, 1 115.335 1, 1 157.357 0, 1 175.369 5, 1 217.373 0, 1 277.370 2, 1 319.404 2, 1 337.423 3, 1 379.390 1, 1 439.431 1, 1 499.477 1
), ArticleFig(id=1200470895111303411, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, language=CN, label=Table 1, caption=

Fragment information of 2-9 sugars in APS-Ⅱ enzymatic hydrolysis by MS/MS. DP: Degree of polymerization

, figureFileSmall=null, figureFileBig=null, tableContent=
DPtR/minMolecular formulaTheoretical value of relative molecular massFragment ions/m/z (M+Na+)
22.17C12H22O11342.296 5203.053 4, 219.059 1, 269.657 9, 305.083 8, 347.095 4, 365.105 7
33.04C18H32O16504.437 1185.040 6, 203.053 3, 347.095 1, 365.106 2, 425.203 7, 467.140 2, 527.158 9
43.87C24H42O21666.577 7185.042 8, 203.053 2, 268.599 6, 347.053 2, 365.106 0, 467.135 7, 509.150 5, 527.160 1, 689.212 2
54.90C30H52O26828.718 3185.042 8, 229.494 2, 268.599 6, 323.433 1, 347.094 5, 365.105 7, 467.137 4, 509.148 2, 527.158 1, 671.202 5, 689.211 9, 851.264 1
66.25C36H62O31990.858 9185.043 2, 203.053 7, 263.064 4, 325.113 2, 347.095 3, 365.105 1, 467.141 7, 483.514 1, 509.148 2, 527.158 5, 671.201 9, 689.210 6, 791.246 3, 833.256 1, 851.265 3, 839.280 1, 953.312 5, 1 013.317 2
77.88C42H72O361 152.999 5185.028 6, 203.039 0, 227.040 8, 245.051 2, 275.062 3, 287.060 4, 305.071 8, 365.105 6, 407.105 1, 467.136 3, 509.147 3, 527.157 7, 569.155 2, 629.185 8, 671.199 3, 689.211 4, 731.210 1, 791.240 4, 833.254 2, 851.263 4, 893.363 3, 908.774 5, 953.281 3, 995.288 7, 1 013.317 4, 1 055.318 3, 1 072.371 2, 1 115.331 3, 1 175.370 2
89.14C48H82O411 315.140 1185.042 6, 219.018 1, 245.051 6, 263.060 2, 272.065 7, 325.113 8, 347.095 1, 365.103 8, 407.106 5, 467.137 3, 509.147 8, 527.158 6, 569.131 5, 629.189 2, 671.200 6, 689.211 6, 731.213 6, 791.251 8, 833.251 9, 851.264 7, 893.286 1, 953.286 1, 1 013.317 3, 1 055.316 3, 1 115.331 2, 1 175.370 7, 1 217.366 7, 1 277.389 5, 1 337.425 3
910.51C54H92O461 477.280 7185.043 1, 191.039 2, 203.052 6, 325.113 7, 347.095 2, 365.106 4, 407.111 2, 467.140 5, 509.148 2, 527.158 8, 569.169 9, 629.193 7, 689.211 9 731.317 2, 759.219 7, 791.238 2, 833.253 1, 851.264 5, 953.293 8, 995.304 8, 1 013.317 0, 1 055.317 2, 1 115.335 1, 1 157.357 0, 1 175.369 5, 1 217.373 0, 1 277.370 2, 1 319.404 2, 1 337.423 3, 1 379.390 1, 1 439.431 1, 1 499.477 1
), ArticleFig(id=1200470895195189499, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, language=EN, label=null, caption=null, figureFileSmall=null, figureFileBig=null, tableContent=
DPtR/minMolecular formulaTheoretical value of relative molecular massFragment ions /m/z (M+Na+)
1011.53C60H102O511 639.421 3127.038 8, 145.049 8, 185.042 6, 191.039 3, 201.007 6, 203.053 9, 213.005 8, 219.018 0, 263.059 5, 272.065 7, 305.085 2, 325.113 6, 347.095 3, 365.105 3, 407.112 9, 467.136 7, 487.165 0, 509.148 6, 527.158 6, 569.132 1 585.132 1, 629.190 1, 671.200 7, 689.211 9, 731.218 6, 791.238 0, 833.254 4, 851.265 6, 953.294 9, 995.306 9, 1 013.315 0, 1 115.349 0, 1 157.357 0, 1 175.370 7, 1 277.394 0, 1 319.416 0, 1 337.428 2, 1 379.451 0, 1 481.478 0, 1 499.467 1, 1 541.484 0, 1 601.523 0, 1 661.532 0
1112.86C66H112O561 801.561 9127.038 9, 145.049 7, 185.043 1, 191.039 1, 203.054 3, 219.017 6, 227.040 5, 245.051 0, 263.060 3, 272.065 1, 305.084 3, 347.095 3, 365.106 7, 407.111 0, 423.078 2, 455.110 5, 467.137 6, 487.164 5, 509.148 4, 510.152 6, 527.159 2, 569.167 5, 629.191 5, 671.200 9, 689.212 0, 731.217 6, 791.242 9, 851.263 9, 893.273 1, 920.780 6, 953.293 4, 1 013.317 0, 1 055.326 0, 1 115.356 0, 1 157.362 0, 1 175.372 0, 1 217.374 0, 1 277.396 0, 1 319.411 0, 1 337.428 0, 1 429.455 0, 1 481.470 0, 1 499.467 0, 1 541.463 0, 1 601.476 0, 1 643.510 2, 1 661.531 0, 1 703.543 0, 1 763.585 0, 1 823.584 0
1213.79C72H122O611 963.704 5145.049 8, 185.042 0, 191.039 6, 203.052 8, 219.017 4, 263.060 4, 272.065 9, 305.085 2, 325.113 3, 347.095 3, 365.105 7, 407.113 7, 423.083 0, 467.138 4, 487.163 7, 509.148 6, 527.159 9, 569.171 2, 629.193 2, 649.209 3, 671.201 3, 689.212 5, 731.226 3, 791.246 0, 833.254 0, 851.264 6, 893.282 7, 953.302 9, 995.309 5, 1 013.318 0, 1 055.325 0, 1 115.350 6, 1 157.360 0, 1 175.373 0, 1 217.373 0, 1 277.408 0, 1 337.413 0, 1 379.390 0, 1 440.488 0, 1 481.462 1, 1 499.488 0, 1 643.535 0, 1 661.524 0, 1 805.591 0, 1 823.591 0, 1 865.567 0, 1 985.646 0
1314.8C78H132O662 125.843 1185.042 4, 245.051 3, 263.059 6, 272.065 3, 325.112 9, 347.095 0, 365.106 1, 381.072 8, 407.109 0, 467.136 6, 473.484 3, 487.167 9, 509.148 3, 527.158 9, 609.818 3, 629.194 8, 649.848 9, 671.201 2, 689.211 1, 731.228 3, 791.246 0, 833.254 7, 851.264 7, 893.274 7, 953.291 0, 1 013.311 7, 1 055.329 0, 1 115.342 0, 1 157.360 0, 1 175.360 0, 1 277.402 0, 1 319.407 0, 1 337.418 0, 1 439.459 0, 1 499.482 0, 1 643.509 0, 1 661.539 0, 1 823.573 0, 1 985.636 0, 2 088.734 0, 2 147.682 0
1416.01C84H142O712 287.893 7159.647 0, 185.042 8, 263.060 3, 269.610 5, 272.063 8, 288.841 7, 301.021 1, 304.885 3, 325.113 8, 344.085 0, 347.095 5, 365.104 4, 487.166 1, 509.148 1, 527.161 6, 629.189 6, 671.201 1, 689.214 1, 791.248 7, 833.255 1, 851.262 8, 886.361 8, 953.293 6, 995.306 3, 1 013.317 0, 1 157.360 0, 1 175.366 0, 1 220.381 0, 1 267.029 0, 1 277.398 0, 1 319.404 3, 1 337.423 0, 1 481.469 0, 1 499.480 0, 1 643.528 0, 1 661.518 0, 1 805.598 0, 1 823.577 0, 1 986.653 0, 2 309.737 0
), ArticleFig(id=1200470895325212936, tenantId=1146029695717560320, journalId=1189982191388893191, articleId=1200394153851998785, language=CN, label=Table 2, caption=

Secondary Fragment Information of 10-14 Sugars

, figureFileSmall=null, figureFileBig=null, tableContent=
DPtR/minMolecular formulaTheoretical value of relative molecular massFragment ions /m/z (M+Na+)
1011.53C60H102O511 639.421 3127.038 8, 145.049 8, 185.042 6, 191.039 3, 201.007 6, 203.053 9, 213.005 8, 219.018 0, 263.059 5, 272.065 7, 305.085 2, 325.113 6, 347.095 3, 365.105 3, 407.112 9, 467.136 7, 487.165 0, 509.148 6, 527.158 6, 569.132 1 585.132 1, 629.190 1, 671.200 7, 689.211 9, 731.218 6, 791.238 0, 833.254 4, 851.265 6, 953.294 9, 995.306 9, 1 013.315 0, 1 115.349 0, 1 157.357 0, 1 175.370 7, 1 277.394 0, 1 319.416 0, 1 337.428 2, 1 379.451 0, 1 481.478 0, 1 499.467 1, 1 541.484 0, 1 601.523 0, 1 661.532 0
1112.86C66H112O561 801.561 9127.038 9, 145.049 7, 185.043 1, 191.039 1, 203.054 3, 219.017 6, 227.040 5, 245.051 0, 263.060 3, 272.065 1, 305.084 3, 347.095 3, 365.106 7, 407.111 0, 423.078 2, 455.110 5, 467.137 6, 487.164 5, 509.148 4, 510.152 6, 527.159 2, 569.167 5, 629.191 5, 671.200 9, 689.212 0, 731.217 6, 791.242 9, 851.263 9, 893.273 1, 920.780 6, 953.293 4, 1 013.317 0, 1 055.326 0, 1 115.356 0, 1 157.362 0, 1 175.372 0, 1 217.374 0, 1 277.396 0, 1 319.411 0, 1 337.428 0, 1 429.455 0, 1 481.470 0, 1 499.467 0, 1 541.463 0, 1 601.476 0, 1 643.510 2, 1 661.531 0, 1 703.543 0, 1 763.585 0, 1 823.584 0
1213.79C72H122O611 963.704 5145.049 8, 185.042 0, 191.039 6, 203.052 8, 219.017 4, 263.060 4, 272.065 9, 305.085 2, 325.113 3, 347.095 3, 365.105 7, 407.113 7, 423.083 0, 467.138 4, 487.163 7, 509.148 6, 527.159 9, 569.171 2, 629.193 2, 649.209 3, 671.201 3, 689.212 5, 731.226 3, 791.246 0, 833.254 0, 851.264 6, 893.282 7, 953.302 9, 995.309 5, 1 013.318 0, 1 055.325 0, 1 115.350 6, 1 157.360 0, 1 175.373 0, 1 217.373 0, 1 277.408 0, 1 337.413 0, 1 379.390 0, 1 440.488 0, 1 481.462 1, 1 499.488 0, 1 643.535 0, 1 661.524 0, 1 805.591 0, 1 823.591 0, 1 865.567 0, 1 985.646 0
1314.8C78H132O662 125.843 1185.042 4, 245.051 3, 263.059 6, 272.065 3, 325.112 9, 347.095 0, 365.106 1, 381.072 8, 407.109 0, 467.136 6, 473.484 3, 487.167 9, 509.148 3, 527.158 9, 609.818 3, 629.194 8, 649.848 9, 671.201 2, 689.211 1, 731.228 3, 791.246 0, 833.254 7, 851.264 7, 893.274 7, 953.291 0, 1 013.311 7, 1 055.329 0, 1 115.342 0, 1 157.360 0, 1 175.360 0, 1 277.402 0, 1 319.407 0, 1 337.418 0, 1 439.459 0, 1 499.482 0, 1 643.509 0, 1 661.539 0, 1 823.573 0, 1 985.636 0, 2 088.734 0, 2 147.682 0
1416.01C84H142O712 287.893 7159.647 0, 185.042 8, 263.060 3, 269.610 5, 272.063 8, 288.841 7, 301.021 1, 304.885 3, 325.113 8, 344.085 0, 347.095 5, 365.104 4, 487.166 1, 509.148 1, 527.161 6, 629.189 6, 671.201 1, 689.214 1, 791.248 7, 833.255 1, 851.262 8, 886.361 8, 953.293 6, 995.306 3, 1 013.317 0, 1 157.360 0, 1 175.366 0, 1 220.381 0, 1 267.029 0, 1 277.398 0, 1 319.404 3, 1 337.423 0, 1 481.469 0, 1 499.480 0, 1 643.528 0, 1 661.518 0, 1 805.598 0, 1 823.577 0, 1 986.653 0, 2 309.737 0
)], attaches=null, journal=Journal(id=1189982048455397383, delFlag=0, nameCn=药学学报, nameEn=Acta Pharmaceutica Sinica, nameHistory1=null, nameHistory2=null, issn=0513-4870, eissn=null, cn=11-2163/R, coden=null, periodic=0, language=CN, oaType=null, ccby=null, superviseOffice=null, ownerOffice=null, pubOffice=null, editorOffice=null, officeType=null, aims=null, clcCode=null, officeProv=null, officeCity=null, officeAddr=null, officeZip=null, officeEmail=null, officePhone=null, editDirector=null, officeDirector=null, officeDirectorPhone=null, officeStaffNum=null, officeEmpNum=null, coverPicUrl=BTxjudbJDVO4PqdBR6On6Q==, journalPrice=null, startedYear=null, abbrevIsoEn=null, journalRemark=null, publicationField=null, createdTime=1761643429151, updatedTime=1761735768113, createdBy=18614031015, updatedBy=13701087609, firstLetterCn=A, firstLetterEn=A, subjectCode=Life Sciences, subjectName=Life Sciences, subjectCodeEn=Life Sciences, subjectNameEn=null, picCn=BTxjudbJDVO4PqdBR6On6Q==, picEn=c4l1ckL55nWbhl1KrFdWIA==, jcr=null, cjcr=null, exts=[JournalExt(id=1190369346338783397, language=CN, name=药学学报, nameHistory1=null, nameHistory2=null, managedBy=, sponsoredBy=, publishedBy=, editorOffice=, officeProv=null, officeCity=null, officeAddr=, officeZip=, editDirector=, officeDirector=null, officePhone=null, coverPicUrl=null, journalRemark=, submitArticleUrl=null, websiteUrl=, createdTime=1761735768160, updatedTime=1761735768160, createdBy=13701087609, updatedBy=13701087609, submissionGuidelinesUrl=, submissionAuthorUrl=https://www.yxxb.com.cn/journalx_yxxb/authorLogOn.action, submissionEditorUrl=https://www.yxxb.com.cn/journalx_yxxb/editorLogOn.action, submissionReviewUrl=https://www.yxxb.com.cn/journalx_yxxb/expertLogOn.action, submissionCeEditorUrl=, submissionAeEditorUrl=, option={"copyright":""}), JournalExt(id=1190369346376532134, language=EN, name=Acta Pharmaceutica Sinica, nameHistory1=null, nameHistory2=null, managedBy=, sponsoredBy=, publishedBy=, editorOffice=, officeProv=null, officeCity=null, officeAddr=, officeZip=, editDirector=, officeDirector=null, officePhone=null, coverPicUrl=null, journalRemark=, submitArticleUrl=null, websiteUrl=, createdTime=1761735768169, updatedTime=1761735768169, createdBy=13701087609, updatedBy=13701087609, submissionGuidelinesUrl=, submissionAuthorUrl=https://www.yxxb.com.cn/journalx_yxxb/authorLogOn.action, submissionEditorUrl=https://www.yxxb.com.cn/journalx_yxxb/editorLogOn.action, submissionReviewUrl=https://www.yxxb.com.cn/journalx_yxxb/expertLogOn.action, submissionCeEditorUrl=, submissionAeEditorUrl=, option={"copyright":""})], databaseList=null, tenantJournalId=1189982191388893191, websiteList=[Website(id=1189982271588340489, webName=null, webTitle=null, webDomain=null, webCopyrigh=null, webIpcNo=null, seoTitle=null, seoKeywords=null, seoDescription=null, tenantJournalId=null, journalId=1189982191388893191, journalNameCn=null, journalNameEn=null, grayFlag=null, tenantId=1146029695717560320, platformId=null, journalGroupId=null, journalGroupNameCn=null, journalGroupNameEn=null, type=1, domain=https://castjournals.cast.org.cn/joweb/yxxb/CN, language=CN, createTime=1761643482348, createBy=18614031015, updateTime=1761643498101, updateBy=18614031015, name=药学学报-中文, tplId=1146099689490845704, title=药学学报, delFlag=0, indexPage=/home, props=[WebsiteProps(id=1189982873114448678, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1189982271588340489, code=articleTextType, value=kx, createTime=1761643625763, updateTime=1761643625763, creator=18614031015, updator=18614031015), WebsiteProps(id=1189982873093477155, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1189982271588340489, code=banner, value=null, createTime=1761643625758, updateTime=1761643625758, creator=18614031015, updator=18614031015), WebsiteProps(id=1189982873135420201, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1189982271588340489, code=grayFlag, value=0, createTime=1761643625768, updateTime=1761643625768, creator=18614031015, updator=18614031015), WebsiteProps(id=1189982873085088546, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1189982271588340489, code=logo, value=https://castjournals.cast.org.cn/joweb/yxxb/CN/file/pic?fileId=w+t2v8bJnX5lh3+hRRJcDA==, createTime=1761643625756, updateTime=1761643625756, creator=18614031015, updator=18614031015), WebsiteProps(id=1189982873152197419, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1189982271588340489, code=minRunFlag, value=0, createTime=1761643625772, updateTime=1761643625772, creator=18614031015, updator=18614031015), WebsiteProps(id=1189982873110254373, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1189982271588340489, code=picServerUrl, value=https://castjournals.cast.org.cn/joweb/yxxb/CN/file/pic, createTime=1761643625762, updateTime=1761643625762, creator=18614031015, updator=18614031015), WebsiteProps(id=1189982873143808810, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1189982271588340489, code=silenceFlag, value=0, createTime=1761643625770, updateTime=1761643625770, creator=18614031015, updator=18614031015), WebsiteProps(id=1189982873101865764, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1189982271588340489, code=staticResourcePath, value=https://castjournals.cast.org.cn/joweb/cast_kjdb_cn_619/, createTime=1761643625760, updateTime=1761643625760, creator=18614031015, updator=18614031015), WebsiteProps(id=1189982873122837287, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1189982271588340489, code=themeColor, value=null, createTime=1761643625765, updateTime=1761643625765, creator=18614031015, updator=18614031015), WebsiteProps(id=1189982873127031592, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1189982271588340489, code=themeStyle, value=null, createTime=1761643625766, updateTime=1761643625766, creator=18614031015, updator=18614031015)]), Website(id=1189982271655449355, webName=null, webTitle=null, webDomain=null, webCopyrigh=null, webIpcNo=null, seoTitle=null, seoKeywords=null, seoDescription=null, tenantJournalId=null, journalId=1189982191388893191, journalNameCn=null, journalNameEn=null, grayFlag=null, tenantId=1146029695717560320, platformId=null, journalGroupId=null, journalGroupNameCn=null, journalGroupNameEn=null, type=1, domain=https://castjournals.cast.org.cn/joweb/yxxb/EN, language=EN, createTime=1761643482364, createBy=18614031015, updateTime=1761643514085, updateBy=18614031015, name=药学学报-英文, tplId=1146101810881728533, title=Acta Pharmaceutica Sinica, delFlag=0, indexPage=/home, props=[WebsiteProps(id=1189982903015633534, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1189982271655449355, code=articleTextType, value=kx, createTime=1761643632892, updateTime=1761643632892, creator=18614031015, updator=18614031015), WebsiteProps(id=1189982902990467707, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1189982271655449355, code=banner, value=null, createTime=1761643632886, updateTime=1761643632886, creator=18614031015, updator=18614031015), WebsiteProps(id=1189982903036605057, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1189982271655449355, code=grayFlag, value=0, createTime=1761643632897, updateTime=1761643632897, creator=18614031015, updator=18614031015), WebsiteProps(id=1189982902982079098, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1189982271655449355, code=logo, value=https://castjournals.cast.org.cn/joweb/yxxb/EN/file/pic?fileId=w+t2v8bJnX5lh3+hRRJcDA==, createTime=1761643632884, updateTime=1761643632884, creator=18614031015, updator=18614031015), WebsiteProps(id=1189982903053382275, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1189982271655449355, code=minRunFlag, value=0, createTime=1761643632901, updateTime=1761643632901, creator=18614031015, updator=18614031015), WebsiteProps(id=1189982903007244925, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1189982271655449355, code=picServerUrl, value=https://castjournals.cast.org.cn/joweb/yxxb/EN/file/pic, createTime=1761643632890, updateTime=1761643632890, creator=18614031015, updator=18614031015), WebsiteProps(id=1189982903044993666, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1189982271655449355, code=silenceFlag, value=0, createTime=1761643632899, updateTime=1761643632899, creator=18614031015, updator=18614031015), WebsiteProps(id=1189982902998856316, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1189982271655449355, code=staticResourcePath, value=https://castjournals.cast.org.cn/joweb/cast_kjdb_en_623/, createTime=1761643632888, updateTime=1761643632888, creator=18614031015, updator=18614031015), WebsiteProps(id=1189982903019827839, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1189982271655449355, code=themeColor, value=null, createTime=1761643632893, updateTime=1761643632893, creator=18614031015, updator=18614031015), WebsiteProps(id=1189982903028216448, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1189982271655449355, code=themeStyle, value=null, createTime=1761643632895, updateTime=1761643632895, creator=18614031015, updator=18614031015)])], journalTitle=药学学报, weixinUrl=null, journalUrl=https://www.yxxb.com.cn/aps, iacademicId=null, status=1, seqNo=null, journalTitleEn=Acta Pharmaceutica Sinica, journalPhotoCn=BTxjudbJDVO4PqdBR6On6Q==, journalPhotoEn=c4l1ckL55nWbhl1KrFdWIA==, journalFirstLetter=A, journalRecommend=null, journalNew=null, journalCollection=null, jcrJf=null, cjcrJf=null, jcrJfStr=null, cjcrJfStr=null, submissionFirstDecision=null, sciSubjectClassification=null, casSubjectClassification=null, citeScore=null, totalCitationFrequency=null, icpCode=null, psCode=null, advertisingLicenseCode=null, copyrightInformation=null, country=null, option=, provinceCode=null, provinceName=null, collectFlag=false), detailUrlCn=https://castjournals.cast.org.cn/joweb/yxxb/CN/10.16438/j.0513-4870.2023-1379, detailUrlEn=https://castjournals.cast.org.cn/joweb/yxxb/EN/10.16438/j.0513-4870.2023-1379, pdfUrlCn=https://castjournals.cast.org.cn/joweb/yxxb/CN/PDF/10.16438/j.0513-4870.2023-1379, pdfUrlEn=https://castjournals.cast.org.cn/joweb/yxxb/EN/PDF/10.16438/j.0513-4870.2023-1379, aliStartDate=null, aliEndDate=null, collectionFlag=false, citedCount=null, citedUrl=null, reference=null)
收藏切换
基于高分辨质谱技术的黄芪多糖APS-Ⅱ酶解活性寡糖特征分支位点的研究
收藏切换
PDF下载
刘宇冲 1, 2, 3 , 李虎峰 1, 2, 3 , 李科 1, 2, 3, 4, * , 秦雪梅 1, 2, 3 , 杜昱光 4 , 李震宇 1, 2, 3
药学学报 | 研究论文 2024,59(7): 2108-2116
收起
收藏切换
药学学报 | 研究论文 2024, 59(7): 2108-2116
基于高分辨质谱技术的黄芪多糖APS-Ⅱ酶解活性寡糖特征分支位点的研究
全屏
刘宇冲1, 2, 3, 李虎峰1, 2, 3, 李科1, 2, 3, 4, * , 秦雪梅1, 2, 3, 杜昱光4, 李震宇1, 2, 3
作者信息
  • 1.山西大学中医药现代研究中心, 山西 太原 030006
  • 2.山西大学化学, 生物学与分子工程教育部重点实验室, 山西 太原 030006
  • 3.地产中药功效物质研究与利用山西省重点实验室, 山西 太原 030006
  • 4.中国科学院过程工程研究所, 北京 100190

通讯作者:

*李科, Tel / Fax: 86-351-7019297, E-mail:
Study on the characteristic branch sites of oligosaccharides of Astragalus polysaccharide APS-Ⅱ enzymolysis based on high resolution mass spectrometry
Yu-chong LIU1, 2, 3, Hu-feng LI1, 2, 3, Ke LI1, 2, 3, 4, * , Xue-mei QIN1, 2, 3, Yu-guang DU4, Zhen-yu LI1, 2, 3
Affiliations
  • 1. Modern Research Center for Traditional Chinese Medicine of Shanxi University, Taiyuan 030006, China
  • 2. Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education of Shanxi University, Taiyuan 030006, China
  • 3. Key Laboratory of Effective Substances Research and Utilization in TCM of Shanxi Province, Taiyuan 030006, China
  • 4. Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China
出版时间: 2024-07-12 doi: 10.16438/j.0513-4870.2023-1379
文章导航
收藏切换
摘要
收起

黄芪多糖是黄芪中免疫调节活性最强, 含量最为丰富的物质, 具有抗肿瘤、抗病毒、免疫促进等多种生物活性, 临床应用广泛。前期研究发现黄芪多糖主要由2种不同相对分子质量多糖APS-Ⅰ (> 2 000 kDa)、APS-Ⅱ (10 kDa) 组成, APS-Ⅱ (10 kDa) 为黄芪多糖中活性最强组分, 并采用α-1, 4-葡聚糖内切酶将APS-Ⅱ降解为寡糖, 通过体外免疫活性筛选发现2~9糖整体免疫活性较低, 而10~14糖具有较强的免疫活性, 且活性优于未降解的APS-Ⅱ。为了探究APS-Ⅱ降解寡糖发挥免疫活性的关键结构, 本文采用MALDI-TOF-MS生物质谱以及高分辨质谱仪器ESI-Q Exactive-MS对APS酶解寡糖进行解析, 通过对比发现10~14糖中存在1→4和1→6两种连接方式共存的支链结构, 推测1→4和1→6两种连接方式共存的支链结构为APS-Ⅱ发挥免疫活性的关键结构, 为黄芪多糖和寡糖的构效关系研究奠定理论基础。

APS-Ⅱ  /  酶解  /  连接方式  /  结构解析  /  高分辨质谱

Astragalus polysaccharides are the most immunoregulatory active and abundant substances in Astragalus, with anti-tumor, anti-viral, and immune-promoting biological activities. They have been widely used in clinical practice. Previous studies have found that Astragalus polysaccharides are mainly composed of two different polysaccharides, APS-Ⅰ (> 2 000 kDa) and APS-Ⅱ (10 kDa), with APS-Ⅱ (10 kDa) being the most active component of Astragalus polysaccharides. We used α-1, 4-glucan endo-hydrolysis enzyme to degrade APS-Ⅱ into oligosaccharides, and screened the immune activity of oligosaccharides in vitro. We found that the overall immune activity of 2-9 oligosaccharides was low, while the immune activity of 10-14 oligosaccharides was strong, and the activity was better than that of untreated APS-Ⅱ. To investigate the key structural features of APS-Ⅱ oligosaccharides that play a role in immune activity, we used MALDI-TOF-MS biochemical mass spectrometry and high-resolution mass spectrometry instrument ESI-Q Exactive-MS to analyze the APS enzymatic oligosaccharides. By comparing, we found that 10-14 oligosaccharides contain 1→4 and 1→6 branched structures with coexisting linkages, suggesting that 1→4 and 1→6 linkages in branched structures are key structural features that play a role in the immune activity of APS-Ⅱ, laying a theoretical foundation for the structure-activity relationship of Astragalus polysaccharides and oligosaccharides.

APS-Ⅱ  /  enzymolysis  /  connection mode  /  structural analysis  /  high resolution mass spectrometry
刘宇冲, 李虎峰, 李科, 秦雪梅, 杜昱光, 李震宇. 基于高分辨质谱技术的黄芪多糖APS-Ⅱ酶解活性寡糖特征分支位点的研究. 药学学报, 2024 , 59 (7) : 2108 -2116 . DOI: 10.16438/j.0513-4870.2023-1379
Yu-chong LIU, Hu-feng LI, Ke LI, Xue-mei QIN, Yu-guang DU, Zhen-yu LI. Study on the characteristic branch sites of oligosaccharides of Astragalus polysaccharide APS-Ⅱ enzymolysis based on high resolution mass spectrometry[J]. Acta Pharmaceutica Sinica, 2024 , 59 (7) : 2108 -2116 . DOI: 10.16438/j.0513-4870.2023-1379
正文
收起
黄芪多糖是黄芪中免疫调节活性最强, 含量最为丰富的物质, 具有抗肿瘤、抗病毒、免疫促进等多种生物活性, 临床应用广泛[1-3]。本文前期研究中通过TSK凝胶色谱分离法进行不同相对分子质量黄芪多糖糖谱表征, 结果表明黄芪多糖主要由2种不同相对分子质量多糖APS-Ⅰ (> 2 000 kDa)、APS-Ⅱ (10 kDa) 组成, 同时基于相对分子质量分布对APS-Ⅰ和APS-Ⅱ进行体内和体外免疫活性筛选, 发现黄芪多糖中活性最强组分为APS-Ⅱ[4, 5]。通过直接对活性多糖APS-Ⅱ进行单糖组成分析和GC-MS单糖连接位点分析得出, APS-Ⅱ主链基本由α-D-1, 4-葡聚糖组成[5, 6], 但由于多糖相对分子质量大, 具体结构研究困难, 因此本文效仿蛋白质结构的研究方式, 采用自下而上的方法, 将多糖降解为寡糖, 并对其进行结构分析。
化学降解法和酶降解法为降解多糖的常用方法, 相较于化学降解, 酶解法可以对特定糖苷键进行切除, 而且反应易于控制, 酶解过程条件温和, 且无副产物生成, 更有利于寡糖的结构研究[7-10]。因此前期采用α-1, 4-葡聚糖内切酶将APS-Ⅱ降解为寡糖, 分别为2~9糖组分和10~14糖组分, 体外免疫活性筛选发现2~9糖组分整体免疫活性较低, 而10~14糖组分具有较强的免疫活性, 且活性优于APS-Ⅱ[11]。诸多研究表明, 寡糖活性的高低与其结构特征直接相关, 因此揭示10~14糖组分与2~9糖组分结构差异对于阐明黄芪多糖药效物质基础具有重要意义。
生物质谱法主要用于生物大分子的质谱解析, 可以对混合物进行直接分析。通常只产生分子离子, 与质量一一对应。能够用于直接反映寡糖片段信息, 具有直观性好、准确度高且不需要对照品等优点[12]。本文前期采用UPLC-Q-TOF负离子模式对APS-Ⅱ酶解寡糖进行分析, 得出2~6糖均为α-1, 4连接的线性葡聚糖, 但具有其他连接方式的糖链未能表征[13], 同时由于Q-TOF-MS仪器质量采集范围的限制导致10糖以上结构未进行解析, 分支结构不明确, 缺少相关糖链序列结构信息。
基质辅助激光解析电离飞行时间质谱(MALDI-TOF-MS) 具有分辨率高、灵敏度高、分析速度快、相对分子质量测量范围大等优势, 广泛应用于多糖结构分析。而ESI-Q Exactive-MS高分辨质谱可对大相对分子质量多糖样品进行定量分析, 同时提供精确的分子质量和结构信息, 具有高通量、高灵敏度和低检出限等优点。通过将MALDI-TOF-MS与ESI-Q Exactive-MS高分辨质谱结合对寡糖进行分析, 可获得更为精确的寡糖结构信息。
基于此, 为了更好地阐明黄芪多糖免疫促进活性的构效关系, 获取更为丰富精确的黄芪寡糖结构信息, 本文采用基质辅助激光解析电离飞行时间质谱(MALDI-TOF-MS) 对APS-Ⅱ酶解寡糖进行分析, 同时结合ESI-Q Exactive-MS高分辨质谱对APS-Ⅱ酶解寡糖进行结构解析。本文首次对APS-Ⅱ酶解寡糖中10糖以上(10~14) 糖链进行高分辨质谱分析, 并通过对比其与2~9糖的结构, 进一步阐明黄芪多糖发挥免疫活性的关键结构, 以期为黄芪多糖和寡糖的构效关系研究提供思路, 为黄芪酶解寡糖的开发利用和黄芪多糖的质量控制研究提供依据。
材料与方法
收起
仪器  ZX-LGJ-18普通型冷冻干燥机(上海知信实验仪器技术有限公司); SC-3610低速离心机(安徽中科中佳科学仪器有限公司); CPA225D分析天平(德国Sartorius公司); RE-52型旋转蒸发器(上海亚荣生化仪器厂); Dionex Ultimate 3000型高效液相色谱仪和Q-Exactive-Orbitrap四极杆轨道阱质谱仪(美国Thermo公司); 基质辅助解析质谱仪MALIDI-TOF-MS (德国Bruker公司); Milli-Q Gradient A 10超纯水仪(密理博上海贸易有限公司); GS-NF500型膜片式切向流膜分离系统(海顾信生物科技有限公司)。
材料与试剂  仿野生黄芪(2019年5月于山西浑源产地采集, 山西大学中医药现代研究中心秦雪梅教授鉴定为豆科植物蒙古黄芪Astragalus membranaceus var. mongholicus); 色谱级乙腈、质谱级甲酸、甲醇、乙腈(美国Thermo公司)。
APS-Ⅱ分离制备  依据参考文献[11]方法, 将粉碎后的干燥黄芪后过100目筛。称取黄芪细粉大约15 g, 以1∶20的料液比加入去离子水后, 搅拌均匀, 在90 ℃下进行水浴提取4 h, 提取过程中每1 h搅拌1次。提取结束后对提取液进行真空抽滤, 并对滤液进行合并。滤液加入200 U木瓜蛋白酶, 搅拌均匀, 在45 ℃下水浴6 h, 加热到90 ℃对其进行高温灭活5 min, 以3 500 r·min-1转速离心5 min。取100 mL上清液加入10%三氯乙酸水溶液至总体积为200 mL, 冰浴15 min后静置30 min进行离心, 再取100 mL上清液, 加入900 mL无水乙醇, 静置过夜。收集醇沉液沉淀进行冷冻干燥, 即得黄芪多糖APS。将APS配制成质量浓度为5 g·L-1溶液, 通过分子截留量为10 kDa的超滤膜得到APS-Ⅱ溶液, 冷冻干燥, 即得APS-Ⅱ。
酶解寡糖的制备  依据文献[4]中酶解方法, 采用内切α-1, 4-葡聚糖糖苷水解酶按照酶浓度为0.5 U·mL-1、酶解温度为60 ℃, 酶解时间90 min的条件对冷冻干燥后的APS-Ⅱ进行酶解。将2 mL APS溶液与2 mL α-1, 4-葡聚糖糖苷水解酶进行混合, 并在60 ℃水浴条件下降解90 min。酶解后, 进行沸水浴10 min, 使酶溶液失活。将溶液进行离心, 并收集上清液, 冷冻干燥, 即得黄芪寡糖APOS。取黄芪寡糖APOS适量, 用去离子水配制成质量浓度为2 mg·mL-1溶液备用。
MALDI-TOF-MS分析  取样品溶液1 mL加入等体积的甲醇溶液充分混匀, 取2 μL溶液点样于MTP Anchorchip 384点的靶板上, 真空抽干。再加1 μL 20 g·L-1的DHB溶液至样品点上, 真空抽干。一级质谱方法参数如下。离子源1: 7.5 kV; 离子源2: 6.75 kV; 反射电压1: 29.5 kV; 反射电压2: 13.95 kV。激发光源为N2激光(337 nm), 分子质量检测范围为500~3 500。
ESI-Q Exactive-MS正离子模式下APS-Ⅱ酶解寡糖2~9结构表征  UPLC-ESI-Q Exactive-MS分析色谱柱: Acquity UPLC BEH Amide (10 mm × 2.1 mm, 1.7 μm); 流动相A为0.1%甲酸水, 流动相B为乙腈; 洗脱程序为0~2 min (68%~65% B), 2~14 min (65%~55% B), 14~20 min (55%~53% B), 20~23 min (53% B), 24~25 min (53%~68% B), 25~29 min (68% B); 流速0.3 mL·min-1; 柱温40 ℃; 样品室温度4 ℃; 进样量2 μL。质谱离子源: 采用电喷雾电离源, 正、负离子模式扫描; 鞘气: 241 kPa; 辅助气: 3.3 L·min-1; 吹扫气: 1 L·min-1; 离子源毛细管温度: 325 ℃; 毛细管电压: -2 500 V; 全扫描模式的质量范围: m/z 200~3 500。
ESI-Q Exactive-MS正离子模式下APS-Ⅱ酶解寡糖10~14糖结构表征  UPLC-ESI-Q Exactive-MS分析色谱柱: Acquity UPLC BEH Amide (10 mm × 2.1 mm, 1.7 μm); 流动相A为0.1%甲酸水, 流动相B为乙腈; 洗脱程序为0~2 min (68%~65% B), 2~14 min (65%~55% B), 14~20 min (55%~53% B), 20~23 min (53% B), 24~25 min (53%~68% B), 25~29 min (68% B); 流速0.3 mL·min-1; 柱温40 ℃; 样品室温度4 ℃; 进样量2 μL。质谱离子源: 电喷雾电离源, 正、负离子模式扫描; 鞘气: 241 kPa; 辅助气: 3.3 L·min-1; 吹扫气: 1 L·min-1; 离子源毛细管温度: 325℃; 毛细管电压: -2 500 V; 全扫描模式的质量范围: m/z 200~3 500。
结果与讨论
收起
1 APS-Ⅱ的酶解及其MALDI-TOF-MS表征
MALDI-TOF-MS具有分辨率高、灵敏度高、相对分子质量测量范围大等优势, 而且所产生的质谱图多为单电荷离子碎片, 质谱图中的离子与寡糖的质量有一一对应关系, 可直接反映寡糖相对分子质量。而ESI-Q Exactive-MS产生的图谱虽然可获得精确的分子质量和结构信息, 但容易产生多电荷离子, 对解析造成干扰, 因此先采用MALDI-TOF-MS对寡糖进行表征, 根据其表征结果对ESI-Q Exactive-MS图谱进行快速准确地分析。
APS-Ⅱ酶解寡糖MALDI-TOF-MS结果如图 1 (DP3为聚合度为3的寡糖, DP4为聚合度为4的寡糖, 以此类推至DP18), 通过分析可得出, MALDI-TOF-MS对酶解寡糖表征后主要产生a、b、c、d、e共5种类型离子。
其中a型离子为[M+Na]+分子离子, 相对分子质量分别为365.204 2、527.226 6、689.251 2、851.290 2、1 013.326 1、1 175.361 9、1 337.397 8、1 499.430 2、1 661.469 8、1 823.512 5、1 985.556 3、2 147.587 8、2 309.608 4、2 471.653 0、2 633.691 2、2 796.788 0、2 957.740 8。通过相对分子质量对比, 可以看出a型分子主要为离子相对分子质量相差162的一系列离子, 基于此可判断a型分子其相差单糖种类为己糖(Hex), 并反映出APS-Ⅱ酶解寡糖链主要为己糖链(Hexn), 且最大聚合度糖链可以达到18糖及以上。而b型离子主要为[M+K]+的己糖链分子离子, c型离子为[M+Na]+ Hexn失去一分子水后产生的离子(Hexn-18)。d型离子为947.226 1 (unknown) +Hexn产生的分子离子, 其相对分子质量分别为947.216 1、1 109.209 4、1 271.308 9、1 433.346 7、1 595.409 2、1 757.429 5、1 919.488 6等, 通过相对分子质量对比, d型离子相对分子质量也相差162, 但947.216 2却不属于己糖链相对分子质量系列, 因此APS-Ⅱ酶解产生了非己糖链的寡糖结构。e型离子与d型离子相似为1 079.721 3 (unknown) +Hexn由非己糖链+己糖链结构构成。相对分子质量为1 079.721 3、1 241.301 8、1 403.356 2、1 565.334 4等相差162的分子离子。通过分析发现, e型离子与d型离子相差了132的相对分子质量, 而该相对分子质量为戊糖形成糖苷键脱水后的相对分子质量, 因此可推测e型系列糖链中存在戊糖。
2 ESI-Q Exactive-MS正离子模式下APS-Ⅱ酶解寡糖2~9结构表征
ESI-Q Exactive-MS高分辨质谱可对大相对分子质量多糖样品进行定量分析, 同时提供精确的相对分子质量和结构信息, 具有高通量、高灵敏度和低检出限等优点。根据上述APS-Ⅱ酶解寡糖MALDI-TOF-MS表征结果, 获得寡糖相对分子质量信息, 通过ESI-Q Exactive-MS对a型离子中2~9进行结构表征, 总离子流如图 2, 2~9糖二级碎片信息见表 1。
2糖分子离子峰为[M+Na+] 型, m/z为365.105 7, 主要断裂方式为糖苷键断裂, 并产生C型203.053 4碎片离子, 同时分子离子脱水会形成347.095 4碎片离子, 除此之外还会发生0,2A2跨环断裂形成305.083 8碎片离子, 主要产生质量数为60的中性丢失, 根据1→4连接葡聚糖裂解规律研究和参考文献[14], 该2糖符合1→4连接的跨环裂解规律, 推测2糖为麦芽糖。
3糖主要断裂形式为糖苷键断裂, 并产生C型203.053 3、365.106 2碎片离子和B型185.040 6、347.095 1碎片离子, 除此之外还发生0,2A2跨环断裂形成467.140 2碎片离子, 符合1→4连接的跨环裂解规律。推测该3糖为麦芽三糖。
4糖主要发生糖苷键断裂, 并产生C型203.053 2、365.106 0、527.160 1碎片离子和B型347.053 2、509.150 5碎片离子, 此外发生0,2A3跨环断裂形成467.135 7碎片离子, 符合1→4连接的跨环裂解规律。推测该4糖为麦芽四糖。
5糖主要发生糖苷键断裂, 并产生C型365.105 7、527.158 1、689.211 9碎片离子和B型347.094 5、509.148 2、671.202 5碎片离子, 除此之外发生0,2A3跨环断裂形成467.137 4碎片离子, 发生0,2A2跨环断裂(305) 后结合一分子水形成323.433 1碎片离子, 符合1→4连接的跨环裂解规律。推测该5糖为麦芽五糖。
6糖主要断裂形式为糖苷键断裂, 并产生C型203.053 7、365.105 1、527.158 5、689.210 6、851.265 3碎片离子和B型185.043 2、347.095 3、509.148 2、671.201 9、833.256 1碎片离子, 除此之外发生了0,2A3、0,2A5、0,2A6跨环断裂形成467.141 7、791.246 3、953.312 5碎片离子, 产生了质量数为60的中性丢失, 符合1→4连接的跨环裂解规律, 同时还发生了0,4A6跨环断裂形成839.280 1碎片, 该碎片离子符合1→6连接裂解规律, 因此还原末端糖C-6位被连接。推测该结构为α-D-Glcp-1→(4-α-D-Glcp-1)4→6-α-D-Glcp。
7糖主要发生糖苷键断裂, 并产生C型203.039 0、365.105 6、527.157 7、689.211 4、851.263 4、1 013.317 4碎片离子和B型185.028 6、509.147 3、671.199 3、833.254 2碎片离子, 此外发生0,2A2、0,2A3、0,2A4、0,2A5、0,2A6、0,2A7跨环断裂形成305.071 8、467.136 3、791.240 4、953.281 3、1 115.331 3碎片离子, 产生质量数为60的中性丢失, 同时还发生0,4A2、0,4A3、0,4A4、0,4A5、0,4A6、0,4A7跨环断裂形成245.051 2、407.105 1、569.155 2、731.210 1、893.363 3、1 055.318 3系列碎片离子, 产生了质量数为60和120的中性丢失, 符合1→6连接裂解规律, 因此推测该7糖为线性的1→6连接葡聚糖, 推测该结构为α-D-Glcp-1→(6-α-D-Glcp-1)5→6-α-D-Glcp。
8糖与7糖结构相似, 主要发生了糖苷键断裂, 并产生C型365.103 8、527.158 6、689.211 6、851.264 7、1 013.317 3、1 175.370 7碎片离子和B型185.042 6、509.147 8、671.200 6、833.251 9碎片离子, 除此之外发生了0,2A3、0,2A4、0,2A5、0,2A6、0,2A7、0,2A8的跨环断裂形成467.137 3、629.189 2、791.251 8、953.286 1、1 115.331 2、1 277.389 5碎片离子, 产生了质量数为60的中性丢失, 同时还发生0,2A3、0,2A4、0,2A5、0,2A6、0,2A7、0,2A8跨环断裂形成245.051 6、407.106 5、569.131 5、731.213 6、893.286 1、1 055.316 3、1 217.366 7系列碎片离子, 产生了质量数为60和120的中性丢失, 符合1→6连接的裂解规律, 因此推测该8糖为线性的1→6连接葡聚糖, 推测该结构为α-D-Glcp-1→(6-α-D-Glcp-1)6→6-α-D-Glcp。
9糖主要断裂形式为糖苷键断裂, 并产生C型203.052 6、365.106 4、527.158 8、689.211 9、851.264 5、1 013.317 0、1 175.369 5、1 337.423 3碎片离子和B型185.043 1、347.095 2、509.148 2、833.253 1、995.304 8、1 157.357 0、1 319.404 2碎片离子, 此外发生了0,2A2、0,2A3、0,2A4、0,2A5、0,2A6、0,2A7、0,2A8跨环断裂形成325.113 7、467.140 5、629.193 7、791.238 2、953.293 8、1 115.335 1、1 277.370 2、1 439.431 1碎片离子, 产生了质量数为60的中性丢失, 同时还发生0,4A3、0,4A4、0,4A5、0,4A7、0,4A8跨环断裂, 并形成407.111 2、569.169 9、731.317 2、1 055.317 2、1 217.373 0、1 379.390 1系列碎片离子, 产生了质量数为120的中性丢失, 符合1→6连接裂解规律。通过分析发现, 其跨环断裂仅产生了0,2A2和0,2A6丢失60的二级碎片离子, 但是并未产生0,4A2和0,4A6丢失120的碎片离子, 由此可得出这两个糖的C-4位被连接, 连接方式为1→4连接。因此推测该9糖为线性1→4连接和1→6连接方式共存的葡聚糖, 并推测该结构为α-D-Glcp-1→4-α-D-Glcp→(6-α-D-Glcp-1)3→4-α-D-Glcp→(6-α-D-Glcp-1)2→6-α-D-Glcp-1。
3 ESI-Q Exactive-MS正离子模式下APS-Ⅱ酶解寡糖10~14糖结构表征
根据APS-Ⅱ酶解寡糖MALDI-TOF-MS表征结果, 获得寡糖相对分子质量信息, 对a型离子中10~14糖进行结构表征, 其二级碎片信息见表 2。
10糖主要发生糖苷键断裂, 并产生C型203.053 9、365.105 3、527.158 6、689.211 9、851.265 6、1 013.315 0、1 175.370 7、1 337.428 2、1 499.467 1碎片离子和B型185.042 6、347.095 3、509.148 6、833.254 4、995.306 9、1 157.357 0、1 319.416 0、1 481.478 0碎片离子, 除此之外发生了0,2A2、0,2A3、0,2A4、0,2A5、0,2A6、0,2A7、0,2A8跨环断裂形成305.085 2、467.136 7、629.190 1、791.238 0、953.294 9、1 115.349 0、1 277.394 0碎片离子, 产生了质量数为60的中性丢失, 同时还发生0,4A3、0,4A4、0,4A5、0,4A9、0,4A10跨环断裂形成407.112 9、569.132 1、731.218 6、1 379.451 0、1 541.484 0系列碎片离子, 产生了质量数为120的中性丢失, 符合1→6连接的裂解规律。通过后续分析发现, 跨环断裂仅产生了0,2A2、0,2A6、0,2A7、0,2A8丢失60的二级碎片, 但并未产生丢失120的碎片离子, 由此可推测出从非还原端起位于2、6、7、8这四个糖的C-4位被连接, 其连接方式为1→4连接。而3、4、5、9、10这五个糖的C-6位被连接, 因此推测该10糖为线性1→4连接和1→6连接方式共存的葡聚糖, 推测该结构为α-D-Glcp-1→4-α-D-Glcp→(6-α-D-Glcp-1)3→(4-α-D-Glcp)3→(6-α-D-Glcp-1)2, 其二级质谱图见图 3。
11糖主要发生糖苷键断裂, 并产生C型203.053 3、365.106 7、527.159 2、689.212 0、851.263 9、1 013.317 0、1 175.372 0、1 337.428 0、1 499.467 0、1 661.531 0碎片离子和B型185.043 1、347.095 3、509.148 4、1 157.362 0、1 319.411 0、1 481.470 0、1 499.467 0、1 661.531 0碎片离子, 除此之外发生了0,2A2、0,2A3、0,2A4、0,2A5、0,2A6、0,2A7、0,2A8、0,2A9、0,2A10、0,2A11跨环断裂形成305.084 3、467.137 6、629.191 5、791.242 9、953.293 4、1 115.356 0、1 277.396 0、1 429.455 0、1 601.476 0、1 763.585 0碎片离子, 产生了质量数为60的中性丢失, 同时还发生了0,4A3、0,4A4、0,4A5、0,4A6、0,4A7、0,4A8、0,2A10、0,2A11跨环断裂形成407.111 0、569.167 5、731.217 6、1 055.326 0、1 217.374 0、1 481.470 0、1 703.543 0系列碎片离子, 产生了质量数为120的中性丢失, 符合1→6连接裂解规律。通过后续分析发现, 跨环断裂仅产生了0.2A2和0,2A9丢失质量数为60的二级碎片, 但并未产生0,4A2和0,4A9丢失120的碎片离子, 由此可得出这两个糖的C-4位被连接, 连接方式为1→4连接。基于此推测该11糖为线性1→4连接和1→6连接方式共存的葡聚糖, 推测该结构为α-D-Glcp-1→4-α-D-Glcp→(6-α-D-Glcp-1)5→(4-α-D-Glcp)2→(6-α-D-Glcp-1)2。
12糖主要发生了糖苷键断裂, 并产生C型203.052 8、365.105 7、527.159 9、689.212 5、851.264 6、1 013.318 0、1 175.373 0、1 337.413 0、1 499.488 0、1 661.524 0、1 823.591 0碎片离子和B型185.042 0、347.095 3、509.148 6、671.201 3、833.254 0、995.309 5、1 157.360 0、1 481.462 1、1 643.535 0、1 805.591 0碎片离子, 除此之外还发生了0,2A2、0,2A3、0,2A4、0,2A5、0,2A6、0,2A7、0,2A8、0,2A9、0,2A10、0,2A11、0,2A12跨环断裂形成305.085 2、467.138 4、629.193 2、791.246 0、953.302 9、1 115.350 6、1 277.408 0、1 440.488 0碎片离子, 产生了质量数为60的中性丢失, 同时 0,4A3、0,4A4、0,4A5、0,4A6、0,4A7、0,4A12的跨环断裂形成了407.113 7、569.171 2、731.226 3、1 055.325 0、1 217.373 0、1 379.390 0系列碎片离子, 产生了质量数为120的中性丢失, 符合1→6连接裂解规律。通过分析发现, 跨环断裂产生了0,2A2、0,2A8、0,2A9、0,2A10、0,2A11丢失质量数为60的二级碎片, 但并未产生0,2A2、0,4A8、0,4A9、0,4A10、0,4A11丢失质量数为120的碎片离子, 由此可得出这5个糖的C-4位被连接, 连接方式为1→4连接。因此推测该12糖为线性1→4连接和1→6连接方式共存的葡聚糖, 推测该结构为α-D-Glcp-1→4-α-D-Glcp→(6-α-D-Glcp-1)5→(4-α-D-Glcp-1)4→6-α-D-Glcp-1。
13糖主要断裂形式为糖苷键断裂, 并产生完整C型碎片离子365.106 1、527.158 9、689.211 1、851.264 7、1 013.311 7、1 175.360 0、1 337.418 0、1 499.482 0、1 661.539 0、1 823.573 0、1 985.636 0和B型185.042 4、347.095 0、509.148 3、671.201 2、833.254 7、1 157.360 0、1 319.407 0、1 643.509 0碎片离子, 除此之外发生了0,2A3、0,2A4、0,2A5、0,2A6、0,2A7、0,2A8、0,2A9、0,2A13跨环断裂形成467.136 6、629.194 8、791.246 0、953.291 0、1 115.342 0、1 277.402 0、1 439.459 0碎片离子, 产生了质量数为60的中性丢失, 同时还发生0,4A2、0,4A3、0,4A5、0,4A6、0,4A7跨环断裂形成245.051 3、407.109 0、731.228 3、893.274 7、1 055.329 0系列碎片离子, 产生了质量数为120的中性丢失, 符合1→6连接的裂解规律。通过分析发现, 跨环断裂仅产生了0,2A4、0,2A8、0,2A9、0,2A13丢失质量数为60的二级碎片, 但并未产生0,4A4、0,4A8、0,4A9、0,4A13丢失120的碎片离子, 由此可得出这4个糖的C-4位被连接, 连接方式为1→4连接。由于寡糖的制备过程中采取了α-1, 4葡聚糖糖苷水解酶进行酶解, 具有一定的专一性, 根据上述实验结果判断出寡糖中存在2~5糖的线性葡聚糖, 因此推测在具有更大相对分子质量或聚合度的糖链中存在2~5糖的结构才能被降解出该糖链, 推测该13糖还原末端5个糖为1→4连接的葡聚糖糖链, 13糖为线性1→4连接和1→6连接方式共存的葡聚糖, 推测其结构为α-D-Glcp-1→(6-α-D-Glcp-1)2→4-α-D-Glcp→(6-α-D-Glcp-1)3→(4-α-D-Glcp-1)6。
14糖主要发生糖苷键断裂, 并产生了C型365.104 4、527.161 6、689.214 1、851.262 8、1 013.317 0、1 175.366 0、1 337.423 0、1 499.480 0、1 661.518 0、1 823.577 0、1 986.653 0、2 309.737 0碎片离子和B型185.042 8、347.095 5、509.148 1、833.255 1、995.306 3、1 157.359 9、1 319.404 3、1 481.469 0、1 643.528 0、1 805.598 0碎片离子, 除此之外发生0,2A2、0,2A3、 0,2A5、0,2A6、0,2A8跨环断裂形成304.885 3、487.166 1、629.189 6、791.248 7、953.293 6、1 277.398 0碎片离子, 产生了质量数为60的中性丢失, 同时还发生0,4A6和0,4A8跨环断裂形成886.361 8、1 220.381 0系列碎片离子, 产生了质量数为120的中性丢失, 符合1→6连接裂解规律, 表明这两个糖6位被连接, 通过分析上述实验结果发现7~13糖中均存在3个1→6连接相连接的片段, 因此推测该糖链中非还原端9糖位置处C-6位被连接, 还原末端6个糖为1→4连接方式。推测该14糖为线性1→4连接和1→6连接方式共存的葡聚糖, 推测该结构为α-D-Glcp-1→(4-α-D-Glcp-1)4→(6-α-D-Glcp-1)3→(4-α-D-Glcp-1)6。二级质谱图见图 4。
其中10糖结构为α-D-Glcp-1→4-α-D-Glcp→(6-α-D-Glcp-1)3→(4-α-D-Glcp)3→(6-α-D-Glcp-1)2, 11糖结构为α-D-Glcp-1→4-α-D-Glcp→(6-α-D-Glcp-1)5→(4-α-D-Glcp)2→(6-α-D-Glcp-1)2, 12糖结构为α-D-Glcp-1→4-α-D-Glcp→(6-α-D-Glcp-1)5→(4-α-D-Glcp-1)4→6-α-D-Glcp-1, 13糖结构为α-D-Glcp-1→(6-α-D-Glcp-1)2→4-α-D-Glcp→(6-α-D-Glcp-1)3→(4-α-D-Glcp-1)6, 14糖结构为α-D-Glcp-1→(4-α-D-Glcp-1)4→(6-α-D-Glcp-1)3→(4-α-D-Glcp-1)6。其中10~14糖共有结构为→(6-α-D-Glcp-1)3→4-α-D-Glcp-1。
基于此, 相较于2~8糖中只存在1→4或1→6连接方式的糖链结构, 在10~14糖中均出现了1→4和1→6两种连接方式共存的分支结构。推测1→4和1→6连接共存的葡聚糖链可能为APS-Ⅱ中发挥免疫促进活性的关键结构。
结论
收起
本文在APS-Ⅱ酶解寡糖负离子模式解析结果[13]前期研究基础上, 采用正离子模式对黄芪活性多糖APS-Ⅱ酶解寡糖进行高分辨质谱解析, 首次对APS-Ⅱ酶解寡糖中10糖以上(10~14) 糖链进行结构解析并与2~9糖结构进行对比, 得出10~14糖共有结构为→(6-α-D-Glcp-1)3→4-α-D-Glcp-1。相比于2~9糖, 10~14糖中均为1→4和1→6两种连接方式共存的糖链。目前有部分研究表明1→4和1→6连接的葡聚糖糖链可激活免疫系统发挥免疫应答[13, 15]。基于此, 本文推测APS-Ⅱ寡糖中1→4和1→6连接共存的糖链可能为APS-Ⅱ中发挥免疫活性的关键结构, 该研究结果可为后续黄芪寡糖及多糖构效关系的研究奠定理论基础和提供技术支撑。
作者贡献: 刘宇冲和李虎峰负责文中实验方案设计; 李虎峰负责实验实施、数据收集、结果分析等工作, 刘宇冲和李虎峰负责文章撰写等工作; 李科负责提供文章思路, 在文章写作方面进行指导和修改; 秦雪梅、杜昱光和李震宇对整个实验内容给予监督, 并对文章进行修改完善。
利益冲突: 所有作者均声明不存在任何利益冲突。
基金
收起
  • 国家自然科学基金资助项目(81872962)
  • 国家博士后科学基金资助项目(2019M650851)
  • 国家重点研发计划(2019YFC1710800)
  • 山西省重点研发计划重点项目(201603D311101)
  • 山西省优秀人才科技创新项目(201605D211030)
  • 山西省优秀人才科技创新项目(201705D211020)
  • 山西省科技创新人才团队专项基金
文献
收起
参考文献 引证文献
排序方式：
[1]
Li Q, Hu J, Gao B, et al. Advances on immunoregulation effect of Astragalus polysaccharides [J]. Chin J Exp Tradit Med Form (中国实验方剂学杂志), 2017, 23: 199-206.
[2]
Morten L, Anette W, Rune L, et al. Enhancement of human adaptive immune responses by administration of a high-molecular-weight polysaccharide extract from the cyanobacterium Arthrospira platensis [J]. J Med Food, 2008, 11: 313-322.
[3]
Jiang C, Tang C, Qiang Y, et al. Immunoregulation effect of Astragalus polysaccharides [J]. Food Sci (食品科学), 2020, 34: 327-332.
[4]
Li K, Cui LJ, Cao YX, et al. UHPLC Q-Exactive MS-based serum metabolomics to explore the effect mechanisms of immunological activity of Astragalus polysaccharides with different molecular weights [J]. Front Pharmacol, 2020, 11: 595692.
[5]
Li K, Cao YX, Jiao SM, et al. Structural characterization and immune activity screening of polysaccharides with different molecular weights from Astragali Radix [J]. Front Pharmacol, 2020, 11: 582091.
[6]
Li K, Li XQ, Li GX, et al. Radix by enzymolysis of endo α-1, 4-glucanas relationship between the structure and immune activity of components from the active polysaccharides APS-Ⅱ of Astragalie [J]. Front Pharmacol, 2022, 13: 839635.
[7]
Li Q. Preparation of Apple Oligosaccharides, Structural Identification and Antitumor Activity (苹果多糖的制备、结构鉴定及抗肿瘤活性) [D]. Xi'an: Fourth Military Medical University, 2013.
[8]
Miu YQ, Gu GP, Wu GR, et al. Research progress on hydrolysis of plant polysaccharides and their products [J]. Chin Wild Plant Resour (中国野生植物资源), 2005, 02: 4-7.
[9]
Li D, Song Y, Wu M, et al. Review on the hydrolysis of plant polysaccharides and the structure of hydrolysate [J]. Food Fermn Ind (食品与发酵工业), 2013, 39: 165-170.
[10]
Li P, Li WY, Cao W, et al. EI candy and its derivative molecular ion ESI-MSN broken behavior preliminary research [J]. Northwest Pharm J (西北药学杂志), 2015, 30: 433-436.
[11]
Li K, Cui LJ, Li XX, et al. Establishment of enzymatic hydrolysis method of active Astragalus polysaccharides APS-Ⅱ and study on the immune activity of oligosaccharides after its degradation [J]. Acta Pharm Sin (药学学报), 2021, 56: 1936-1944.
[12]
Wang S, Chen C, Guan M, et al. Terminal epitope-dependent branch preference of siglecs toward N-glycans [J]. Front Mol Biosci, 2021, 8: 645999.
[13]
Cui LJ. Astragalus Active Polysaccharide APS-Ⅱ. Immune Regulation Mechanism and Structure and Activity of Enzymatic Hydrolyzable Oligosaccharide Fragments (黄芪活性多糖APS-Ⅱ免疫调控机制及其酶解寡糖片段的结构和活性研究) [D]. Taiyuan: Shanxi University, 2021.
[14]
Wang Z. Preliminary Studies on Derivatived Oligosaccharide Molecular Ions Fragmentation Pattern in ESI-MSn Analysis (ESI-MSn分析中衍生寡糖分子离子裂解模式的初步研究) [D]. Xi'an: Northwest University, 2011.
[15]
He BL, Zheng QW, Guo LQ, et al. Structural characterization and immune-enhancing activity of a novel high-molecular-weight polysaccharide from Cordyceps militaris [J]. Int J Biol Macromol, 2020, 145: 11-20.
2024年第59卷第7期
PDF下载
153
63
引用本文
BibTeX
文章信息
doi: 10.16438/j.0513-4870.2023-1379
  • 接收时间：2023-12-11
  • 首发时间：2025-11-26
  • 出版时间：2024-07-12
补充材料
相关文章
文章信息
作者
出版历史
  • 收稿日期：2023-12-11
  • 修回日期：2024-05-11
基金
国家自然科学基金资助项目(81872962)
国家博士后科学基金资助项目(2019M650851)
国家重点研发计划(2019YFC1710800)
山西省重点研发计划重点项目(201603D311101)
山西省优秀人才科技创新项目(201605D211030)
山西省优秀人才科技创新项目(201705D211020)
山西省科技创新人才团队专项基金
作者信息
    1.山西大学中医药现代研究中心, 山西 太原 030006
    2.山西大学化学, 生物学与分子工程教育部重点实验室, 山西 太原 030006
    3.地产中药功效物质研究与利用山西省重点实验室, 山西 太原 030006
    4.中国科学院过程工程研究所, 北京 100190

通讯作者:

*李科, Tel / Fax: 86-351-7019297, E-mail:
参考文献
分享链接
https://castjournals.cast.org.cn/joweb/yxxb/CN/10.16438/j.0513-4870.2023-1379
分享至
全文二维码

扫描看全文

引用本文
BibTeX
本文的引用情况
2种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
关闭全屏
相关链接
论文
最新文章
热读文章
热点专题
内参
蓝皮书
产业发展报告
传播力报告
期刊分级目录
资讯
学术新闻
行业新闻
学术会议
行业会议
关于
关于我们
联系我们
北京市海淀区学院南路86号
100081
010-62199257
qkjq@cast.org.cn

中国科学技术协会版权所有 京ICP 备10216604号-4 海淀分局备案 1101084647
科技导报社主办 中国科协信息中心技术支持