药学学报

Compd.	Yield/%	Melting/℃	ESI-MS m/z [M+H]⁺
Ⅰ₁	50.4	176.3-177.7	656.29
Ⅰ₂	49.2	175.0-176.3	671.24
Ⅰ₃	55.6	171.4-172.7	684.51
Ⅰ₄	57.5	172.8-174.2	698.62
Ⅰ₅	58.1	168.5-170.0	712.52
Ⅱ₁	48.2	169.5-170.4	670.52
Ⅱ₂	50.6	170.2-171.1	684.37
Ⅱ₃	47.2	167.8-168.5	698.79
Ⅱ₄	50.8	165.2-166.3	712.64
Ⅱ₅	46.1	162.7-163.8	726.82

Compd.

Yield/%

Melting/℃

ESI-MS m/z [M+H]⁺

Ⅰ₁

50.4

176.3-177.7

656.29

Ⅰ₂

49.2

175.0-176.3

671.24

Ⅰ₃

55.6

171.4-172.7

684.51

Ⅰ₄

57.5

172.8-174.2

698.62

Ⅰ₅

58.1

168.5-170.0

712.52

Ⅱ₁

48.2

169.5-170.4

670.52

Ⅱ₂

50.6

170.2-171.1

684.37

Ⅱ₃

47.2

167.8-168.5

698.79

Ⅱ₄

50.8

165.2-166.3

712.64

Ⅱ₅

46.1

162.7-163.8

726.82

Compd.	Yield/%	Melting/℃	ESI-MS m/z [M+H]⁺
Ⅰ₁	50.4	176.3-177.7	656.29
Ⅰ₂	49.2	175.0-176.3	671.24
Ⅰ₃	55.6	171.4-172.7	684.51
Ⅰ₄	57.5	172.8-174.2	698.62
Ⅰ₅	58.1	168.5-170.0	712.52
Ⅱ₁	48.2	169.5-170.4	670.52
Ⅱ₂	50.6	170.2-171.1	684.37
Ⅱ₃	47.2	167.8-168.5	698.79
Ⅱ₄	50.8	165.2-166.3	712.64
Ⅱ₅	46.1	162.7-163.8	726.82

Compd.

Yield/%

Melting/℃

ESI-MS m/z [M+H]⁺

Ⅰ₁

50.4

176.3-177.7

656.29

Ⅰ₂

49.2

175.0-176.3

671.24

Ⅰ₃

55.6

171.4-172.7

684.51

Ⅰ₄

57.5

172.8-174.2

698.62

Ⅰ₅

58.1

168.5-170.0

712.52

Ⅱ₁

48.2

169.5-170.4

670.52

Ⅱ₂

50.6

170.2-171.1

684.37

Ⅱ₃

47.2

167.8-168.5

698.79

Ⅱ₄

50.8

165.2-166.3

712.64

Ⅱ₅

46.1

162.7-163.8

726.82

Compd.	¹H NMR (600 MHz, CDCl₃)	¹³C NMR (150 MHz, CDCl₃)
3-1	δ_H 5.28 (s, 1H, 12-H), 4.40 (t, J = 7.0 Hz, 1H, 3-H), 2.89 (dd, J = 11.2, 4.2 Hz, 1H, H-18), 2.97(s, 1H), 2.06 (s, 3H), 1.03-2.04 (m, 20H), 0.90-1.07 (m, 2 H), 1.05 (s, 3H), 0.93 (s, 3H), 0.91 (s, 3H), 0.90 (s, 3H), 0.85 (s, 3H), 0.82 (s, 3H), 0.79 (s, 3H)	δ_C 179.5, 172.6, 143.3, 123.8, 83.1, 81.0, 55.2, 52.0, 47.6, 46.4, 46.1, 43.0, 42.4, 40.2, 39.8, 38.2, 37.8, 35.7, 32.4, 31.5, 30.4, 29.2, 27.2, 25.0, 24.3, 23.8, 23.5, 23.4, 22.0, 19.6, 17.3, 16.7, 16.2
3-2	δ_H 5.30 (s, 1H, 12-H), 4.45 (t, J = 7.2 Hz, 1H, 3-H), 2.85 (dd, J = 12.0, 4.8 Hz, 1H, H-18), 2.95 (s, 1H), 2.27-2.30 (m, 2H), 1.01-2.07 (m, 20H), 0.97-1.09 (m, 2 H), 1.19 (s, 3H), 1.02 (s, 3H), 0.92 (s, 3H), 0.91 (s, 3H), 0.90 (s, 3H), 0.87 (s, 3H), 0.84 (s, 3H), 0.81 (s, 3H)	δ_C 179.0, 174.3, 143.9, 124.3, 82.4, 80.3, 55.6, 52.4, 47.3, 46.7, 46.5, 42.7, 42.1, 39.7, 39.1, 37.9, 37.6, 36.2, 32.9, 31.2, 29.4, 28.3, 26.7, 24.7, 24.0, 23.5, 23.4, 23.1, 21.6, 19.0, 17.7, 16.9, 16.4, 9.7
3-3	δ_H 5.27 (s, 1H, 12-H), 4.42 (t, J = 7.2 Hz, 1H, 3-H), 2.85 (dd, J = 10.6, 4.0 Hz, 1H, H-18), 2.98 (s, 1H), 1.83-2.35 (m, 4H), 1.05-2.02 (m, 20H), 0.95-1.12 (m, 2 H), 1.05 (s, 3H), 1.02 (s, 3H), 1.00 (s, 3H), 0.95 (s, 3H), 0.92 (s, 3H), 0.88 (s, 3H), 0.87 (s, 3H), 0.84 (s, 3H)	δ_C 179.7, 174.0, 140.1, 123.1, 83.5, 81.1, 55.0, 52.1, 47.5, 46.2, 43.1, 42.3, 40.1, 39.2, 38.7, 38.5, 37.2, 37.0, 36.2, 36.0, 33.2, 30.8, 28.8, 28.0, 27.2, 24.2, 23.8, 23.5, 23.0, 22.6, 21.6, 18.5, 18.1, 16.1, 14.0
3-4	δ_H 5.32 (s, 1H, 12-H), 4.38 (t, J = 7.0 Hz, 1 H, 3-H), 2.85 (dd, J = 12.2, 4.6 Hz, 1H, H-18), 2.92 (s, 1H), 1.40-2.32 (m, 6H), 1.10-2.13 (m, 20H), 0.97-1.15 (m, 2H), 1.08 (s, 3H), 1.05 (s, 3H), 0.97 (s, 3H), 0.93 (s, 3H), 0.92 (s, 3H), 0.89 (s, 3H), 0.87 (s, 3H), 0.82 (s, 3H)	δ_C 180.2, 173.2, 139.5, 123.4, 83.7, 80.6, 55.6, 52.4, 47.1, 46.6, 42.8, 41.7, 41.2, 39.9, 38.2, 37.4, 37.0, 36.8, 36.4, 36.2, 34.2, 31.2, 29.3, 28.5, 26.9, 24.5, 23.8, 23.6, 23.1, 22.3, 21.2, 18.4, 17.7, 16.9, 16.6, 13.5
3-5	δ_H 5.26 (s, 1H, 12-H), 4.38 (t, J = 7.0 Hz, 1H, 3-H), 2.85 (dd, J = 10.4, 4.0 Hz, 1H, H-18), 2.95 (s, 1H), 1.25-2.36 (m, 8H), 1.12-2.21 (m, 20H), 0.94-1.14 (m, 2H), 1.10 (s, 3H), 1.04 (s, 3H), 1.02 (s, 3H), 0.97 (s, 3H), 0.93 (s, 3H), 0.92 (s, 3H), 0.88 (s, 3H), 0.87 (s, 3H)	δ_C 180.0, 173.5, 138.2, 123.0, 84.0, 81.2, 55.2, 52.0, 47.7, 46.2, 43.1, 42.0, 40.6, 39.7, 39.2, 37.8, 37.5, 37.0, 36.4, 36.0, 33.7, 31.6, 31.2, 29.0, 28.9, 26.3, 24.1, 24.0, 23.8, 23.4, 22.1, 21.6, 18.1, 17.2, 16.7, 16.0, 14.2
5-1	δ_H 5.32 (s, 1H, 12-H), 4.45 (t, J = 7.4 Hz, 1H, 3-H), 4.15-4.21 (m, 2H), 2.82 (s, 1H), 2.55 (dd, J = 10.8, 4.0 Hz, 1H, H-18), 1.10-2.11 (m, 20H), 1.23 (s, 3H), 1.10 (s, 3H), 1, 02 (s, 3H), 0.90 (s, 3H), 0.89 (s, 3H), 0.88 (s, 3H), 0.85 (s, 3H), 0.82 (s, 3H)	δ_C 177.5, 160.2, 140.2, 123.3, 84.1, 75.1, 74.6, 61.3, 55.7, 52.8, 48.6, 47.1, 42.8, 40.5, 39.5, 39.0, 38.9, 37.8, 37.5, 37.0, 33.2, 30.7, 29.1, 26.5, 24.6, 24.0, 23.8, 23.6, 23.2, 21.4, 18.9, 17.6, 17.0, 16.5, 15.1
5-2	δ_H 5.28 (s, 1H, 12-H), 4.42 (t, J = 8.0 Hz, 1H, 3-H), 4.10-4.20 (m, 2H), 2.74 (s, 1H), 2.52 (dd, J = 9.4, 4.2 Hz, 1H, H-18), 1.06-2.14 (m, 20H), 1.68-1.74 (m, 2H), 1.10 (s, 3H), 1, 02 (s, 3H), 0.98 (s, 3H), 0.90 (s, H), 0.88 (s, 3H), 0.86 (s, 3H), 0.85 (s, 3H), 0.83 (s, 3H)	δ_C 177.0, 155.6, 139.1, 123.0, 83.5, 74.9, 73.8, 65.7, 55.7, 53.2, 48.6, 47.5, 42.2, 40.2, 39.1, 38.7, 38.5, 37.6, 37.3, 36.8, 33.5, 30.5, 29.4, 26.0, 24.3, 23.9, 23.7, 23.5, 23.0, 21.9, 21.4, 18.5, 17.6, 17.2, 16.2, 10.4
5-3	δ_H 5.21 (s, 1H, 12-H), 4.40 (t, J = 8.2 Hz, 1H, 3-H), 4.05-4.12 (m, 2H), 2.70 (s, 1H), 2.50 (dd, J = 10.8, 4.4 Hz, 1H, H-18), 1.10-2.18 (m, 20H), 1.37-1.55 (m, 4H), 1.07 (s, 3H), 1, 03 (s, 3H), 1.01 (s, 3H), 0.92 (s, H), 0.90 (s, 3H), 0.88 (s, 3H), 0.86 (s, 3H), 0.81 (s, 3H)	δ_C 177.5, 154.0, 138.3, 123.4, 84.3, 74.5, 73.9, 65.3, 55.2, 53.7, 48.4, 47.2, 42.6, 40.5, 39.4, 39.2, 38.5, 37.8, 37.6, 35.9, 34.7, 32.4, 30.2, 28.8, 27.2, 24.5, 24.0, 23.5, 22.6, 22.3, 21.6, 21.5, 18.2, 18.1, 17.5, 16.4, 14.0
5-4	δ_H 5.30 (s, 1H, 12-H), 4.42 (t, J = 7.6 Hz, 1H, 3-H), 4.02-4.10 (m, 2H), 2.74 (s, 1H), 2.56 (dd, J = 12.0, 4.4 Hz, 1H, H-18), 1.05-2.13 (m, 20H), 1.39-1.61 (m, 6H), 1.02 (s, 3H), 0.99 (s, 3H), 0.98 (s, 3H), 0.90 (s, H), 0.89 (s, 3H), 0.88 (s, 3H), 0.86 (s, 3H), 0.84 (s, 3H)	δ_C 177.2, 153.5, 138.0, 124.0, 83.8, 74.2, 73.9, 65.8, 55.6, 53.3, 49.2, 48.5, 44.1, 41.2, 40.2, 39.5, 39.2, 37.2, 36.4, 35.3, 34.2, 30.1, 29.0, 28.4, 27.9, 27.0, 24.3, 23.9, 22.8, 22.5, 22.1, 21.6, 21.4, 18.9, 18.8, 18.2, 16.2, 14.2
5-5	δ_H 5.23 (s, 1H, 12-H), 4.46 (t, J = 7.0 Hz, 1H, 3-H), 4.02-4.08 (m, 2H), 2.70 (s, 1H), 2.56 (dd, J = 12.2, 4.8 Hz, 1H, H-18), 1.12-2.25 (m, 20H), 1.36-1.65 (m, 8H), 1.04 (s, 3H), 0.98 (s, 3H), 0.97 (s, 3H), 0.89 (s, H), 0.87 (s, 3H), 0.85 (s, 3H), 0.80 (s, 3H), 0.78 (s, 3H)	δ_C 177.6, 154.0, 138.5, 123.5, 84.2, 74.5, 74.0, 66.0, 56.2, 54.2, 50.1, 48.3, 45.4, 42.1, 41.8, 40.5, 39.7, 39.2, 38.2, 36.2, 35.1, 31.7, 29.3, 29.2, 28.4, 28.0, 27.5, 24.1, 23.6, 23.4, 22.7, 22.3, 21.5, 21.4, 19.3, 19.2, 18.6, 17.0, 12.7
Ⅰ₁	δ_H 7.79 (s, 1H, triazole-H), 7.05-7.53 (m, 5H), 5.31 (s, 1H, 12-H), 5.20 (d, J = 2.0 Hz, 2H), 4.49 (t, J = 7.2 Hz, 1 H, 3-H), 2.93 (dd, J = 11.0, 4.6 Hz, 1H, H-18), 2.06 (s, 3H), 1.07-2.01 (m, 20H), 0.94-1.05 (m, 2H), 1.02 (s, 3H), 0.91 (s, 3H), 0.90 (s, 3H), 0.89 (s, 3H), 0.86 (s, 3H), 0.85 (s, 3H), 0.82 (s, 3H)	δ_C 179.5, 174.2, 144.7, 134.4, 131.2, 128.4, 128.4, 127.0, 127.0, 122.9, 80.4, 63.1, 55.1, 47.3, 46.7, 46.4, 43.1, 42.0, 39.3, 38.0, 37.7, 36.1, 35.8, 34.1, 32.8, 32.7, 32.2, 30.6, 29.8, 28.0, 27.9, 25.6, 25.6, 23.7, 23.5, 23.5, 21.1, 18.0, 16.9, 16.6, 15.3
Ⅰ₂	δ_H 7.81 (s, 1H, triazole-H), 7.02-7.50 (m, 5H), 5.32 (s, 1H, 12-H), 5.19 (d, J = 3.2 Hz, 2H), 4.50 (t, J = 7.9 Hz, 1H, 3-H), 2.89 (dd, J = 9.2, 4.0 Hz, 1H, H-18), 2.31-2.27 (m, 2H), 1.04-2.05 (m, 20H), 1.22 (s, 3H), 0.94-1.07 (m, 2H), 1.01 (s, 3H), 0.91 (s, 3H), 0.90 (s, 3H), 0.87 (s, 3H), 0.87 (s, 3H), 0.85 (s, 3H), 0.80 (s, 3H)	δ_C 178.5, 171.0, 144.8, 132.3, 132.2, 126.1 126.1, 126.0, 126.0, 122.8, 115.8, 115.6, 80.8, 63.9, 55.1, 53.2, 47.4, 46.7, 46.5, 42.1, 42.0, 39.3, 38.8, 38.1, 37.7, 36.7, 34.1, 33.0, 32.6, 32.2, 28.0, 27.3, 25.7, 23.8, 23.6, 23.5, 23.4, 21.3, 18.1, 16.8, 16.6, 15.2
Ⅰ₃	δ_H 7.84 (s, 1H, triazole-H), 7.05-7.58 (m, 5H), 5.32 (s, 1H, 12-H), 5.23 (d, J = 3.0 Hz, 2H), 4.52 (t, J = 7.2 Hz, 1H, 3-H), 2.85 (dd, J = 8.0, 4.0 Hz, 1H, H-18), 2.35-2.29 (m, 2H), 1.06-2.01 (m, 20H), 1.61-1.68 (m, 2H), 1.60 (m, 2H), 1.23 (s, 3H), 1.15 (s, 3H), 0.95-1.07 (m, 2H), 0.94 (s, 3H), 0.89 (s, 3H), 0.87 (s, 3H), 0.85 (s, 3H), 0.84 (s, 3H), 0.78 (s, 3H)	δ_C 178.4, 170.9, 144.7, 132.7, 132.7, 132.7 132.2, 132.1, 127.7, 125.7, 125.7, 125.4, 125.4, 80.7, 55.0, 47.3, 46.6, 46.3, 42.0, 41.9, 39.2, 38.7, 38.0, 37.7, 37.5, 36.6, 32.8, 32.5, 32.1, 30.6, 27.9, 27.1, 26.8, 25.6, 23.7, 23.4, 23.4, 23.2, 21.2, 18.0, 16.7, 16.5, 15.1
Ⅰ₄	δ_H 7.82 (s, 1H, triazole-H), 7.01-7.50 (m, 5H), 5.32 (s, 1H, 12-H), 5.21 (d, J = 3.4 Hz, 2H), 4.51 (t, J = 7.5 Hz, 1 H, 3-H), 2.83 (dd, J = 8.0, 4.0 Hz, 1H, H-18), 2.33-2.25 (m, 2H), 1.01-2.05 (m, 20H), 1.35-1.80 (m, 4H), 1.25 (s, 3H), 1.16 (s, 3H), 0.92-1.04 (m, 2 H), 1.02 (s, 3H), 0.92 (s, 3H), 0.89 (s, 3H), 0.87 (s, 3H), 0.86 (s, 3H), 0.79 (s, 3H)	δ_C 178.5, 174.3, 144.7, 138.7, 134.9, 132.7, 131.2, 128.0, 128.0, 127.2, 127.2, 122.9, 80.5, 64.6, 55.1, 47.4, 46.7, 46.4, 42.1, 42.1, 42.0, 39.3, 38.6, 38.1, 37.8, 36.8, 34.1, 33.0, 32.6, 32.2, 32.2, 30.7, 28.1, 28.0, 27.3, 23.8, 23.8, 23.5, 23.4, 21.2, 18.1, 16.8, 16.7, 15.3
Ⅰ₅	δ_H 7.87 (s, 1H, triazole-H), 7.03-7.55 (m, 5H), 5.29 (s, 1H, 12-H), 5.19 (d, J = 3.2 Hz, 2H), 4.49 (t, J = 7.2 Hz, 1 H, 3-H), 2.82 (dd, J = 8.4, 4.3 Hz, 1H, H-18), 2.34-2.26 (m, 2H), 1.04-2.08 (m, 20H), 1.26-1.78 (m, 6H), 1.21 (s, 3H), 1.18 (s, 3H), 0.90-1.06 (m, 2 H), 0.96 (s, 3H), 0.93 (s, 3H), 0.89 (s, 3H), 0.86 (s, 3H), 0.85(s, 3H), 0.76 (s, 3H)	δ_C 179.7, 174.4, 144.9, 134.7, 131.4, 128.7, 128.7, 127.2, 127.2, 123.1, 80.6, 63.3, 55.3, 47.6, 46.9, 46.6, 42.2, 42.2, 39.5, 38.3, 37.9, 36.3, 36.0, 34.3, 33.1, 33.0, 32.4, 32.4, 30.8, 30.1, 28.2, 28.1, 27.5, 27.5, 25.8, 25.8, 23.9, 23.7, 23.6, 18.3, 17.1, 16.8, 16.8, 15.5, 9.5
Ⅱ₁	δ_H 7.88 (s, 1H, triazole-H), 7.10-7.55 (m, 5H), 5.43 (s, 1H, 12-H), 4.41 (t, J = 8.0 Hz, 1H, 3-H), 4.25-4.19 (m, 2H), 3.63 (t, J = 7.2 Hz, 2H), 2.58 (dd, J = 12.0, 4.0 Hz, 1H, H-18), 1.01-2.02 (m, 20H), 1.20 (s, 3H), 1.15 (s, 3H), 0.92-1.08 (m, 2H), 0.98 (s, 3H), 0.91 (s, 3H), 0.89 (s, 3H), 0.84 (s, 3H), 0.80(s, 3H), 0.71 (s, 3H)	δ_C 179.5, 174.2, 144.7, 133.5, 131.2, 128.4, 128.4, 127.0, 127.0, 125.9, 125.9, 80.4, 64.4, 55.1, 47.4, 46.7, 46.4, 42.1, 42.0, 42.0, 39.3, 38.0, 37.7, 36.8, 36.1, 35.8, 32.9, 32.8, 32.2, 30.6, 29.9, 28.0, 27.9, 25.6, 23.7, 23.5, 23.4, 21.3, 21.1, 18.6, 16.9, 15.3
Ⅱ₂	δ_H 7.83 (s, 1H, triazole-H), 7.03-7.53 (m, 5H), 5.21 (s, 1H, 12-H), 4.29 (t, J = 8.2 Hz, 1H, 3-H), 4.24-4.17 (m, 2H), 3.60 (t, J = 7.0 Hz, 2H), 2.55 (dd, J = 12.4, 3.5 Hz, 1H, H-18), 1.03-2.06 (m, 20H), 1.72 (m, 2 H), 1.19 (s, 3H), 1.14 (s, 3H), 0.90-1.10 (m, 2H), 0.98 (s, 3H), 0.93 (s, 3H), 0.91 (s, 3H), 0.89 (s, 3H), 0.87 (s, 3H), 0.85 (s, 3H)	δ_C 178.6, 171.2, 144.9, 132.4, 132.4 132.3, 132.3, 126.2, 126.2, 126.1, 125.9, 121.9, 121.9, 121.8, 80.9, 64.0, 55.2, 47.5, 46.9, 46.6, 42.2, 42.1, 39.4, 39.0, 37.8, 36.9, 34.2, 33.1, 32.7, 32.3, 30.8, 28.1, 27.3, 26.7, 25.8, 23.9, 23.6, 23.6, 23.5, 18.2, 16.9, 15.3, 9.33
Ⅱ₃	δ_H 7.86 (s, 1H, triazole-H), 7.09-7.45 (m, 5H), 5.24 (s, 1H, 12-H), 4.23 (t, J = 8.2 Hz, 1 H, 3-H), 4.20-4.10 (m, 2H), 3.58 (t, J = 7.2 Hz, 2H), 2.52 (dd, J = 12.0, 4.5 Hz, 1H, H-18), 1.05-2.10 (m, 20H), 1.32-1.59 (m, 4H), 1.15 (s, 3H), 1.10 (s, 3H), 0.91-1.07 (m, 2H), 0.95 (s, 3H), 0.93 (s, 3H), 0.90 (s, 3H), 0.89 (s, 3H), 0.87 (s, 3H), 0.83 (s, 3H)	δ_C 179.4, 171.0, 144.7, 136.4, 134.2, 131.8, 130.7, 130.0, 130.0, 127.4, 127.4, 122.9, 121.4, 119.7, 80.8, 65.0, 55.2, 47.5, 46.7, 46.4, 42.1, 39.4, 39.4, 38.1, 37.7, 36.9, 36.5, 34.1, 30.7, 29.9, 29.9, 28.0, 27.3, 25.7, 25.7, 24.8, 23.7, 23.6, 23.6, 21.3, 18.2, 17.0, 16.7, 15.4
Ⅱ₄	δ_H 7.84 (s, 1H, triazole-H), 7.13-7.50 (m, 5H), 5.31 (s, 1H, 12-H), 4.26 (t, J = 8.0 Hz, 1H, 3-H), 4.23-4.12 (m, 2H), 3.55 (t, J = 7.2 Hz, 2H), 2.50 (dd, J = 12.0, 4.0 Hz, 1H, H-18), 1.02-2.14 (m, 20H), 1.35-1.69 (m, 6H), 1.12 (s, 3H), 0.95-1.05 (m, 2H), 0.99 (s, 3H), 0.98 (s, 3H), 0.94 (s, 3H), 0.91 (s, 3H), 0.89 (s, 3H), 0.79 (s, 3H), 0.77 (s, 3H)	δ_C 179.6, 174.3, 144.8, 134.6, 131.3, 128.5, 128.5, 127.1, 127.1, 123.0, 80.5, 65.2, 55.2, 47.5, 46.8, 46.5, 42.1, 42.1, 39.4, 39.4, 38.1, 37.8, 36.9, 36.2, 35.9, 33.0, 32.9, 30.7, 30.0, 30.0, 28.1, 27.4, 26.8, 25.7, 25.7, 23.8, 23.6, 23.5, 18.2, 18.2, 17.0, 16.7, 15.4, 15.4, 9.4
Ⅱ₅	δ_H 7.91 (s, 1H, triazole-H), 7.10-7.59 (m, 5H), 5.43 (s, 1H, 12-H), 4.32 (t, J = 8.2 Hz, 1H, 3-H), 4.30-4.16 (m, 2H), 3.58 (t, J = 7.2 Hz, 2H), 2.55 (dd, J = 12.4, 4.2 Hz, 1H, H-18), 1.01-2.07 (m, 20H), 1.28-1.65 (m, 8H), 1.10 (s, 3H), 0.94-1.06 (m, 2H), 0.99 (s, 3H), 0.98 (s, 3H), 0.97 (s, 3H), 0.91 (s, 3H), 0.90 (s, 3H), 0.79 (s, 3H), 0.75 (s, 3H)	δ_C 179.4, 171.1, 144.7, 136.4, 134.2, 131.8, 130.7, 130.7, 130.0, 130.0, 127.4, 127.4, 122.9, 80.9, 66.2, 55.2, 47.5, 46.7, 46.4, 42.1, 42.1, 42.0, 39.4, 38.1, 37.7, 36.9, 36.5, 34.1, 33.0, 32.9, 32.9, 30.7, 30.0, 28.0, 27.3, 25.7, 24.8, 23.7, 21.3, 21.3, 18.2, 17.0, 16.7, 15.4, 15.4, 9.4

Compd.

¹H NMR (600 MHz, CDCl₃)

¹³C NMR (150 MHz, CDCl₃)

3-1

δ_H 5.28 (s, 1H, 12-H), 4.40 (t, J = 7.0 Hz, 1H, 3-H), 2.89 (dd, J = 11.2, 4.2 Hz, 1H, H-18), 2.97(s, 1H), 2.06 (s, 3H), 1.03-2.04 (m, 20H), 0.90-1.07 (m, 2 H), 1.05 (s, 3H), 0.93 (s, 3H), 0.91 (s, 3H), 0.90 (s, 3H), 0.85 (s, 3H), 0.82 (s, 3H), 0.79 (s, 3H)

δ_C 179.5, 172.6, 143.3, 123.8, 83.1, 81.0, 55.2, 52.0, 47.6, 46.4, 46.1, 43.0, 42.4, 40.2, 39.8, 38.2, 37.8, 35.7, 32.4, 31.5, 30.4, 29.2, 27.2, 25.0, 24.3, 23.8, 23.5, 23.4, 22.0, 19.6, 17.3, 16.7, 16.2

3-2

δ_H 5.30 (s, 1H, 12-H), 4.45 (t, J = 7.2 Hz, 1H, 3-H), 2.85 (dd, J = 12.0, 4.8 Hz, 1H, H-18), 2.95 (s, 1H), 2.27-2.30 (m, 2H), 1.01-2.07 (m, 20H), 0.97-1.09 (m, 2 H), 1.19 (s, 3H), 1.02 (s, 3H), 0.92 (s, 3H), 0.91 (s, 3H), 0.90 (s, 3H), 0.87 (s, 3H), 0.84 (s, 3H), 0.81 (s, 3H)

δ_C 179.0, 174.3, 143.9, 124.3, 82.4, 80.3, 55.6, 52.4, 47.3, 46.7, 46.5, 42.7, 42.1, 39.7, 39.1, 37.9, 37.6, 36.2, 32.9, 31.2, 29.4, 28.3, 26.7, 24.7, 24.0, 23.5, 23.4, 23.1, 21.6, 19.0, 17.7, 16.9, 16.4, 9.7

3-3

δ_H 5.27 (s, 1H, 12-H), 4.42 (t, J = 7.2 Hz, 1H, 3-H), 2.85 (dd, J = 10.6, 4.0 Hz, 1H, H-18), 2.98 (s, 1H), 1.83-2.35 (m, 4H), 1.05-2.02 (m, 20H), 0.95-1.12 (m, 2 H), 1.05 (s, 3H), 1.02 (s, 3H), 1.00 (s, 3H), 0.95 (s, 3H), 0.92 (s, 3H), 0.88 (s, 3H), 0.87 (s, 3H), 0.84 (s, 3H)

δ_C 179.7, 174.0, 140.1, 123.1, 83.5, 81.1, 55.0, 52.1, 47.5, 46.2, 43.1, 42.3, 40.1, 39.2, 38.7, 38.5, 37.2, 37.0, 36.2, 36.0, 33.2, 30.8, 28.8, 28.0, 27.2, 24.2, 23.8, 23.5, 23.0, 22.6, 21.6, 18.5, 18.1, 16.1, 14.0

3-4

δ_H 5.32 (s, 1H, 12-H), 4.38 (t, J = 7.0 Hz, 1 H, 3-H), 2.85 (dd, J = 12.2, 4.6 Hz, 1H, H-18), 2.92 (s, 1H), 1.40-2.32 (m, 6H), 1.10-2.13 (m, 20H), 0.97-1.15 (m, 2H), 1.08 (s, 3H), 1.05 (s, 3H), 0.97 (s, 3H), 0.93 (s, 3H), 0.92 (s, 3H), 0.89 (s, 3H), 0.87 (s, 3H), 0.82 (s, 3H)

δ_C 180.2, 173.2, 139.5, 123.4, 83.7, 80.6, 55.6, 52.4, 47.1, 46.6, 42.8, 41.7, 41.2, 39.9, 38.2, 37.4, 37.0, 36.8, 36.4, 36.2, 34.2, 31.2, 29.3, 28.5, 26.9, 24.5, 23.8, 23.6, 23.1, 22.3, 21.2, 18.4, 17.7, 16.9, 16.6, 13.5

3-5

δ_H 5.26 (s, 1H, 12-H), 4.38 (t, J = 7.0 Hz, 1H, 3-H), 2.85 (dd, J = 10.4, 4.0 Hz, 1H, H-18), 2.95 (s, 1H), 1.25-2.36 (m, 8H), 1.12-2.21 (m, 20H), 0.94-1.14 (m, 2H), 1.10 (s, 3H), 1.04 (s, 3H), 1.02 (s, 3H), 0.97 (s, 3H), 0.93 (s, 3H), 0.92 (s, 3H), 0.88 (s, 3H), 0.87 (s, 3H)

δ_C 180.0, 173.5, 138.2, 123.0, 84.0, 81.2, 55.2, 52.0, 47.7, 46.2, 43.1, 42.0, 40.6, 39.7, 39.2, 37.8, 37.5, 37.0, 36.4, 36.0, 33.7, 31.6, 31.2, 29.0, 28.9, 26.3, 24.1, 24.0, 23.8, 23.4, 22.1, 21.6, 18.1, 17.2, 16.7, 16.0, 14.2

5-1

δ_H 5.32 (s, 1H, 12-H), 4.45 (t, J = 7.4 Hz, 1H, 3-H), 4.15-4.21 (m, 2H), 2.82 (s, 1H), 2.55 (dd, J = 10.8, 4.0 Hz, 1H, H-18), 1.10-2.11 (m, 20H), 1.23 (s, 3H), 1.10 (s, 3H), 1, 02 (s, 3H), 0.90 (s, 3H), 0.89 (s, 3H), 0.88 (s, 3H), 0.85 (s, 3H), 0.82 (s, 3H)

δ_C 177.5, 160.2, 140.2, 123.3, 84.1, 75.1, 74.6, 61.3, 55.7, 52.8, 48.6, 47.1, 42.8, 40.5, 39.5, 39.0, 38.9, 37.8, 37.5, 37.0, 33.2, 30.7, 29.1, 26.5, 24.6, 24.0, 23.8, 23.6, 23.2, 21.4, 18.9, 17.6, 17.0, 16.5, 15.1

5-2

δ_H 5.28 (s, 1H, 12-H), 4.42 (t, J = 8.0 Hz, 1H, 3-H), 4.10-4.20 (m, 2H), 2.74 (s, 1H), 2.52 (dd, J = 9.4, 4.2 Hz, 1H, H-18), 1.06-2.14 (m, 20H), 1.68-1.74 (m, 2H), 1.10 (s, 3H), 1, 02 (s, 3H), 0.98 (s, 3H), 0.90 (s, H), 0.88 (s, 3H), 0.86 (s, 3H), 0.85 (s, 3H), 0.83 (s, 3H)

δ_C 177.0, 155.6, 139.1, 123.0, 83.5, 74.9, 73.8, 65.7, 55.7, 53.2, 48.6, 47.5, 42.2, 40.2, 39.1, 38.7, 38.5, 37.6, 37.3, 36.8, 33.5, 30.5, 29.4, 26.0, 24.3, 23.9, 23.7, 23.5, 23.0, 21.9, 21.4, 18.5, 17.6, 17.2, 16.2, 10.4

5-3

δ_H 5.21 (s, 1H, 12-H), 4.40 (t, J = 8.2 Hz, 1H, 3-H), 4.05-4.12 (m, 2H), 2.70 (s, 1H), 2.50 (dd, J = 10.8, 4.4 Hz, 1H, H-18), 1.10-2.18 (m, 20H), 1.37-1.55 (m, 4H), 1.07 (s, 3H), 1, 03 (s, 3H), 1.01 (s, 3H), 0.92 (s, H), 0.90 (s, 3H), 0.88 (s, 3H), 0.86 (s, 3H), 0.81 (s, 3H)

δ_C 177.5, 154.0, 138.3, 123.4, 84.3, 74.5, 73.9, 65.3, 55.2, 53.7, 48.4, 47.2, 42.6, 40.5, 39.4, 39.2, 38.5, 37.8, 37.6, 35.9, 34.7, 32.4, 30.2, 28.8, 27.2, 24.5, 24.0, 23.5, 22.6, 22.3, 21.6, 21.5, 18.2, 18.1, 17.5, 16.4, 14.0

5-4

δ_H 5.30 (s, 1H, 12-H), 4.42 (t, J = 7.6 Hz, 1H, 3-H), 4.02-4.10 (m, 2H), 2.74 (s, 1H), 2.56 (dd, J = 12.0, 4.4 Hz, 1H, H-18), 1.05-2.13 (m, 20H), 1.39-1.61 (m, 6H), 1.02 (s, 3H), 0.99 (s, 3H), 0.98 (s, 3H), 0.90 (s, H), 0.89 (s, 3H), 0.88 (s, 3H), 0.86 (s, 3H), 0.84 (s, 3H)

δ_C 177.2, 153.5, 138.0, 124.0, 83.8, 74.2, 73.9, 65.8, 55.6, 53.3, 49.2, 48.5, 44.1, 41.2, 40.2, 39.5, 39.2, 37.2, 36.4, 35.3, 34.2, 30.1, 29.0, 28.4, 27.9, 27.0, 24.3, 23.9, 22.8, 22.5, 22.1, 21.6, 21.4, 18.9, 18.8, 18.2, 16.2, 14.2

5-5

δ_H 5.23 (s, 1H, 12-H), 4.46 (t, J = 7.0 Hz, 1H, 3-H), 4.02-4.08 (m, 2H), 2.70 (s, 1H), 2.56 (dd, J = 12.2, 4.8 Hz, 1H, H-18), 1.12-2.25 (m, 20H), 1.36-1.65 (m, 8H), 1.04 (s, 3H), 0.98 (s, 3H), 0.97 (s, 3H), 0.89 (s, H), 0.87 (s, 3H), 0.85 (s, 3H), 0.80 (s, 3H), 0.78 (s, 3H)

δ_C 177.6, 154.0, 138.5, 123.5, 84.2, 74.5, 74.0, 66.0, 56.2, 54.2, 50.1, 48.3, 45.4, 42.1, 41.8, 40.5, 39.7, 39.2, 38.2, 36.2, 35.1, 31.7, 29.3, 29.2, 28.4, 28.0, 27.5, 24.1, 23.6, 23.4, 22.7, 22.3, 21.5, 21.4, 19.3, 19.2, 18.6, 17.0, 12.7

Ⅰ₁

δ_H 7.79 (s, 1H, triazole-H), 7.05-7.53 (m, 5H), 5.31 (s, 1H, 12-H), 5.20 (d, J = 2.0 Hz, 2H), 4.49 (t, J = 7.2 Hz, 1 H, 3-H), 2.93 (dd, J = 11.0, 4.6 Hz, 1H, H-18), 2.06 (s, 3H), 1.07-2.01 (m, 20H), 0.94-1.05 (m, 2H), 1.02 (s, 3H), 0.91 (s, 3H), 0.90 (s, 3H), 0.89 (s, 3H), 0.86 (s, 3H), 0.85 (s, 3H), 0.82 (s, 3H)

δ_C 179.5, 174.2, 144.7, 134.4, 131.2, 128.4, 128.4, 127.0, 127.0, 122.9, 80.4, 63.1, 55.1, 47.3, 46.7, 46.4, 43.1, 42.0, 39.3, 38.0, 37.7, 36.1, 35.8, 34.1, 32.8, 32.7, 32.2, 30.6, 29.8, 28.0, 27.9, 25.6, 25.6, 23.7, 23.5, 23.5, 21.1, 18.0, 16.9, 16.6, 15.3

Ⅰ₂

δ_H 7.81 (s, 1H, triazole-H), 7.02-7.50 (m, 5H), 5.32 (s, 1H, 12-H), 5.19 (d, J = 3.2 Hz, 2H), 4.50 (t, J = 7.9 Hz, 1H, 3-H), 2.89 (dd, J = 9.2, 4.0 Hz, 1H, H-18), 2.31-2.27 (m, 2H), 1.04-2.05 (m, 20H), 1.22 (s, 3H), 0.94-1.07 (m, 2H), 1.01 (s, 3H), 0.91 (s, 3H), 0.90 (s, 3H), 0.87 (s, 3H), 0.87 (s, 3H), 0.85 (s, 3H), 0.80 (s, 3H)

δ_C 178.5, 171.0, 144.8, 132.3, 132.2, 126.1 126.1, 126.0, 126.0, 122.8, 115.8, 115.6, 80.8, 63.9, 55.1, 53.2, 47.4, 46.7, 46.5, 42.1, 42.0, 39.3, 38.8, 38.1, 37.7, 36.7, 34.1, 33.0, 32.6, 32.2, 28.0, 27.3, 25.7, 23.8, 23.6, 23.5, 23.4, 21.3, 18.1, 16.8, 16.6, 15.2

Ⅰ₃

δ_H 7.84 (s, 1H, triazole-H), 7.05-7.58 (m, 5H), 5.32 (s, 1H, 12-H), 5.23 (d, J = 3.0 Hz, 2H), 4.52 (t, J = 7.2 Hz, 1H, 3-H), 2.85 (dd, J = 8.0, 4.0 Hz, 1H, H-18), 2.35-2.29 (m, 2H), 1.06-2.01 (m, 20H), 1.61-1.68 (m, 2H), 1.60 (m, 2H), 1.23 (s, 3H), 1.15 (s, 3H), 0.95-1.07 (m, 2H), 0.94 (s, 3H), 0.89 (s, 3H), 0.87 (s, 3H), 0.85 (s, 3H), 0.84 (s, 3H), 0.78 (s, 3H)

δ_C 178.4, 170.9, 144.7, 132.7, 132.7, 132.7 132.2, 132.1, 127.7, 125.7, 125.7, 125.4, 125.4, 80.7, 55.0, 47.3, 46.6, 46.3, 42.0, 41.9, 39.2, 38.7, 38.0, 37.7, 37.5, 36.6, 32.8, 32.5, 32.1, 30.6, 27.9, 27.1, 26.8, 25.6, 23.7, 23.4, 23.4, 23.2, 21.2, 18.0, 16.7, 16.5, 15.1

Ⅰ₄

δ_H 7.82 (s, 1H, triazole-H), 7.01-7.50 (m, 5H), 5.32 (s, 1H, 12-H), 5.21 (d, J = 3.4 Hz, 2H), 4.51 (t, J = 7.5 Hz, 1 H, 3-H), 2.83 (dd, J = 8.0, 4.0 Hz, 1H, H-18), 2.33-2.25 (m, 2H), 1.01-2.05 (m, 20H), 1.35-1.80 (m, 4H), 1.25 (s, 3H), 1.16 (s, 3H), 0.92-1.04 (m, 2 H), 1.02 (s, 3H), 0.92 (s, 3H), 0.89 (s, 3H), 0.87 (s, 3H), 0.86 (s, 3H), 0.79 (s, 3H)

δ_C 178.5, 174.3, 144.7, 138.7, 134.9, 132.7, 131.2, 128.0, 128.0, 127.2, 127.2, 122.9, 80.5, 64.6, 55.1, 47.4, 46.7, 46.4, 42.1, 42.1, 42.0, 39.3, 38.6, 38.1, 37.8, 36.8, 34.1, 33.0, 32.6, 32.2, 32.2, 30.7, 28.1, 28.0, 27.3, 23.8, 23.8, 23.5, 23.4, 21.2, 18.1, 16.8, 16.7, 15.3

Ⅰ₅

δ_H 7.87 (s, 1H, triazole-H), 7.03-7.55 (m, 5H), 5.29 (s, 1H, 12-H), 5.19 (d, J = 3.2 Hz, 2H), 4.49 (t, J = 7.2 Hz, 1 H, 3-H), 2.82 (dd, J = 8.4, 4.3 Hz, 1H, H-18), 2.34-2.26 (m, 2H), 1.04-2.08 (m, 20H), 1.26-1.78 (m, 6H), 1.21 (s, 3H), 1.18 (s, 3H), 0.90-1.06 (m, 2 H), 0.96 (s, 3H), 0.93 (s, 3H), 0.89 (s, 3H), 0.86 (s, 3H), 0.85(s, 3H), 0.76 (s, 3H)

δ_C 179.7, 174.4, 144.9, 134.7, 131.4, 128.7, 128.7, 127.2, 127.2, 123.1, 80.6, 63.3, 55.3, 47.6, 46.9, 46.6, 42.2, 42.2, 39.5, 38.3, 37.9, 36.3, 36.0, 34.3, 33.1, 33.0, 32.4, 32.4, 30.8, 30.1, 28.2, 28.1, 27.5, 27.5, 25.8, 25.8, 23.9, 23.7, 23.6, 18.3, 17.1, 16.8, 16.8, 15.5, 9.5

Ⅱ₁

δ_H 7.88 (s, 1H, triazole-H), 7.10-7.55 (m, 5H), 5.43 (s, 1H, 12-H), 4.41 (t, J = 8.0 Hz, 1H, 3-H), 4.25-4.19 (m, 2H), 3.63 (t, J = 7.2 Hz, 2H), 2.58 (dd, J = 12.0, 4.0 Hz, 1H, H-18), 1.01-2.02 (m, 20H), 1.20 (s, 3H), 1.15 (s, 3H), 0.92-1.08 (m, 2H), 0.98 (s, 3H), 0.91 (s, 3H), 0.89 (s, 3H), 0.84 (s, 3H), 0.80(s, 3H), 0.71 (s, 3H)

δ_C 179.5, 174.2, 144.7, 133.5, 131.2, 128.4, 128.4, 127.0, 127.0, 125.9, 125.9, 80.4, 64.4, 55.1, 47.4, 46.7, 46.4, 42.1, 42.0, 42.0, 39.3, 38.0, 37.7, 36.8, 36.1, 35.8, 32.9, 32.8, 32.2, 30.6, 29.9, 28.0, 27.9, 25.6, 23.7, 23.5, 23.4, 21.3, 21.1, 18.6, 16.9, 15.3

Ⅱ₂

δ_H 7.83 (s, 1H, triazole-H), 7.03-7.53 (m, 5H), 5.21 (s, 1H, 12-H), 4.29 (t, J = 8.2 Hz, 1H, 3-H), 4.24-4.17 (m, 2H), 3.60 (t, J = 7.0 Hz, 2H), 2.55 (dd, J = 12.4, 3.5 Hz, 1H, H-18), 1.03-2.06 (m, 20H), 1.72 (m, 2 H), 1.19 (s, 3H), 1.14 (s, 3H), 0.90-1.10 (m, 2H), 0.98 (s, 3H), 0.93 (s, 3H), 0.91 (s, 3H), 0.89 (s, 3H), 0.87 (s, 3H), 0.85 (s, 3H)

δ_C 178.6, 171.2, 144.9, 132.4, 132.4 132.3, 132.3, 126.2, 126.2, 126.1, 125.9, 121.9, 121.9, 121.8, 80.9, 64.0, 55.2, 47.5, 46.9, 46.6, 42.2, 42.1, 39.4, 39.0, 37.8, 36.9, 34.2, 33.1, 32.7, 32.3, 30.8, 28.1, 27.3, 26.7, 25.8, 23.9, 23.6, 23.6, 23.5, 18.2, 16.9, 15.3, 9.33

Ⅱ₃

δ_H 7.86 (s, 1H, triazole-H), 7.09-7.45 (m, 5H), 5.24 (s, 1H, 12-H), 4.23 (t, J = 8.2 Hz, 1 H, 3-H), 4.20-4.10 (m, 2H), 3.58 (t, J = 7.2 Hz, 2H), 2.52 (dd, J = 12.0, 4.5 Hz, 1H, H-18), 1.05-2.10 (m, 20H), 1.32-1.59 (m, 4H), 1.15 (s, 3H), 1.10 (s, 3H), 0.91-1.07 (m, 2H), 0.95 (s, 3H), 0.93 (s, 3H), 0.90 (s, 3H), 0.89 (s, 3H), 0.87 (s, 3H), 0.83 (s, 3H)

δ_C 179.4, 171.0, 144.7, 136.4, 134.2, 131.8, 130.7, 130.0, 130.0, 127.4, 127.4, 122.9, 121.4, 119.7, 80.8, 65.0, 55.2, 47.5, 46.7, 46.4, 42.1, 39.4, 39.4, 38.1, 37.7, 36.9, 36.5, 34.1, 30.7, 29.9, 29.9, 28.0, 27.3, 25.7, 25.7, 24.8, 23.7, 23.6, 23.6, 21.3, 18.2, 17.0, 16.7, 15.4

Ⅱ₄

δ_H 7.84 (s, 1H, triazole-H), 7.13-7.50 (m, 5H), 5.31 (s, 1H, 12-H), 4.26 (t, J = 8.0 Hz, 1H, 3-H), 4.23-4.12 (m, 2H), 3.55 (t, J = 7.2 Hz, 2H), 2.50 (dd, J = 12.0, 4.0 Hz, 1H, H-18), 1.02-2.14 (m, 20H), 1.35-1.69 (m, 6H), 1.12 (s, 3H), 0.95-1.05 (m, 2H), 0.99 (s, 3H), 0.98 (s, 3H), 0.94 (s, 3H), 0.91 (s, 3H), 0.89 (s, 3H), 0.79 (s, 3H), 0.77 (s, 3H)

δ_C 179.6, 174.3, 144.8, 134.6, 131.3, 128.5, 128.5, 127.1, 127.1, 123.0, 80.5, 65.2, 55.2, 47.5, 46.8, 46.5, 42.1, 42.1, 39.4, 39.4, 38.1, 37.8, 36.9, 36.2, 35.9, 33.0, 32.9, 30.7, 30.0, 30.0, 28.1, 27.4, 26.8, 25.7, 25.7, 23.8, 23.6, 23.5, 18.2, 18.2, 17.0, 16.7, 15.4, 15.4, 9.4

Ⅱ₅

δ_H 7.91 (s, 1H, triazole-H), 7.10-7.59 (m, 5H), 5.43 (s, 1H, 12-H), 4.32 (t, J = 8.2 Hz, 1H, 3-H), 4.30-4.16 (m, 2H), 3.58 (t, J = 7.2 Hz, 2H), 2.55 (dd, J = 12.4, 4.2 Hz, 1H, H-18), 1.01-2.07 (m, 20H), 1.28-1.65 (m, 8H), 1.10 (s, 3H), 0.94-1.06 (m, 2H), 0.99 (s, 3H), 0.98 (s, 3H), 0.97 (s, 3H), 0.91 (s, 3H), 0.90 (s, 3H), 0.79 (s, 3H), 0.75 (s, 3H)

δ_C 179.4, 171.1, 144.7, 136.4, 134.2, 131.8, 130.7, 130.7, 130.0, 130.0, 127.4, 127.4, 122.9, 80.9, 66.2, 55.2, 47.5, 46.7, 46.4, 42.1, 42.1, 42.0, 39.4, 38.1, 37.7, 36.9, 36.5, 34.1, 33.0, 32.9, 32.9, 30.7, 30.0, 28.0, 27.3, 25.7, 24.8, 23.7, 21.3, 21.3, 18.2, 17.0, 16.7, 15.4, 15.4, 9.4

Compd.	¹H NMR (600 MHz, CDCl₃)	¹³C NMR (150 MHz, CDCl₃)
3-1	δ_H 5.28 (s, 1H, 12-H), 4.40 (t, J = 7.0 Hz, 1H, 3-H), 2.89 (dd, J = 11.2, 4.2 Hz, 1H, H-18), 2.97(s, 1H), 2.06 (s, 3H), 1.03-2.04 (m, 20H), 0.90-1.07 (m, 2 H), 1.05 (s, 3H), 0.93 (s, 3H), 0.91 (s, 3H), 0.90 (s, 3H), 0.85 (s, 3H), 0.82 (s, 3H), 0.79 (s, 3H)	δ_C 179.5, 172.6, 143.3, 123.8, 83.1, 81.0, 55.2, 52.0, 47.6, 46.4, 46.1, 43.0, 42.4, 40.2, 39.8, 38.2, 37.8, 35.7, 32.4, 31.5, 30.4, 29.2, 27.2, 25.0, 24.3, 23.8, 23.5, 23.4, 22.0, 19.6, 17.3, 16.7, 16.2
3-2	δ_H 5.30 (s, 1H, 12-H), 4.45 (t, J = 7.2 Hz, 1H, 3-H), 2.85 (dd, J = 12.0, 4.8 Hz, 1H, H-18), 2.95 (s, 1H), 2.27-2.30 (m, 2H), 1.01-2.07 (m, 20H), 0.97-1.09 (m, 2 H), 1.19 (s, 3H), 1.02 (s, 3H), 0.92 (s, 3H), 0.91 (s, 3H), 0.90 (s, 3H), 0.87 (s, 3H), 0.84 (s, 3H), 0.81 (s, 3H)	δ_C 179.0, 174.3, 143.9, 124.3, 82.4, 80.3, 55.6, 52.4, 47.3, 46.7, 46.5, 42.7, 42.1, 39.7, 39.1, 37.9, 37.6, 36.2, 32.9, 31.2, 29.4, 28.3, 26.7, 24.7, 24.0, 23.5, 23.4, 23.1, 21.6, 19.0, 17.7, 16.9, 16.4, 9.7
3-3	δ_H 5.27 (s, 1H, 12-H), 4.42 (t, J = 7.2 Hz, 1H, 3-H), 2.85 (dd, J = 10.6, 4.0 Hz, 1H, H-18), 2.98 (s, 1H), 1.83-2.35 (m, 4H), 1.05-2.02 (m, 20H), 0.95-1.12 (m, 2 H), 1.05 (s, 3H), 1.02 (s, 3H), 1.00 (s, 3H), 0.95 (s, 3H), 0.92 (s, 3H), 0.88 (s, 3H), 0.87 (s, 3H), 0.84 (s, 3H)	δ_C 179.7, 174.0, 140.1, 123.1, 83.5, 81.1, 55.0, 52.1, 47.5, 46.2, 43.1, 42.3, 40.1, 39.2, 38.7, 38.5, 37.2, 37.0, 36.2, 36.0, 33.2, 30.8, 28.8, 28.0, 27.2, 24.2, 23.8, 23.5, 23.0, 22.6, 21.6, 18.5, 18.1, 16.1, 14.0
3-4	δ_H 5.32 (s, 1H, 12-H), 4.38 (t, J = 7.0 Hz, 1 H, 3-H), 2.85 (dd, J = 12.2, 4.6 Hz, 1H, H-18), 2.92 (s, 1H), 1.40-2.32 (m, 6H), 1.10-2.13 (m, 20H), 0.97-1.15 (m, 2H), 1.08 (s, 3H), 1.05 (s, 3H), 0.97 (s, 3H), 0.93 (s, 3H), 0.92 (s, 3H), 0.89 (s, 3H), 0.87 (s, 3H), 0.82 (s, 3H)	δ_C 180.2, 173.2, 139.5, 123.4, 83.7, 80.6, 55.6, 52.4, 47.1, 46.6, 42.8, 41.7, 41.2, 39.9, 38.2, 37.4, 37.0, 36.8, 36.4, 36.2, 34.2, 31.2, 29.3, 28.5, 26.9, 24.5, 23.8, 23.6, 23.1, 22.3, 21.2, 18.4, 17.7, 16.9, 16.6, 13.5
3-5	δ_H 5.26 (s, 1H, 12-H), 4.38 (t, J = 7.0 Hz, 1H, 3-H), 2.85 (dd, J = 10.4, 4.0 Hz, 1H, H-18), 2.95 (s, 1H), 1.25-2.36 (m, 8H), 1.12-2.21 (m, 20H), 0.94-1.14 (m, 2H), 1.10 (s, 3H), 1.04 (s, 3H), 1.02 (s, 3H), 0.97 (s, 3H), 0.93 (s, 3H), 0.92 (s, 3H), 0.88 (s, 3H), 0.87 (s, 3H)	δ_C 180.0, 173.5, 138.2, 123.0, 84.0, 81.2, 55.2, 52.0, 47.7, 46.2, 43.1, 42.0, 40.6, 39.7, 39.2, 37.8, 37.5, 37.0, 36.4, 36.0, 33.7, 31.6, 31.2, 29.0, 28.9, 26.3, 24.1, 24.0, 23.8, 23.4, 22.1, 21.6, 18.1, 17.2, 16.7, 16.0, 14.2
5-1	δ_H 5.32 (s, 1H, 12-H), 4.45 (t, J = 7.4 Hz, 1H, 3-H), 4.15-4.21 (m, 2H), 2.82 (s, 1H), 2.55 (dd, J = 10.8, 4.0 Hz, 1H, H-18), 1.10-2.11 (m, 20H), 1.23 (s, 3H), 1.10 (s, 3H), 1, 02 (s, 3H), 0.90 (s, 3H), 0.89 (s, 3H), 0.88 (s, 3H), 0.85 (s, 3H), 0.82 (s, 3H)	δ_C 177.5, 160.2, 140.2, 123.3, 84.1, 75.1, 74.6, 61.3, 55.7, 52.8, 48.6, 47.1, 42.8, 40.5, 39.5, 39.0, 38.9, 37.8, 37.5, 37.0, 33.2, 30.7, 29.1, 26.5, 24.6, 24.0, 23.8, 23.6, 23.2, 21.4, 18.9, 17.6, 17.0, 16.5, 15.1
5-2	δ_H 5.28 (s, 1H, 12-H), 4.42 (t, J = 8.0 Hz, 1H, 3-H), 4.10-4.20 (m, 2H), 2.74 (s, 1H), 2.52 (dd, J = 9.4, 4.2 Hz, 1H, H-18), 1.06-2.14 (m, 20H), 1.68-1.74 (m, 2H), 1.10 (s, 3H), 1, 02 (s, 3H), 0.98 (s, 3H), 0.90 (s, H), 0.88 (s, 3H), 0.86 (s, 3H), 0.85 (s, 3H), 0.83 (s, 3H)	δ_C 177.0, 155.6, 139.1, 123.0, 83.5, 74.9, 73.8, 65.7, 55.7, 53.2, 48.6, 47.5, 42.2, 40.2, 39.1, 38.7, 38.5, 37.6, 37.3, 36.8, 33.5, 30.5, 29.4, 26.0, 24.3, 23.9, 23.7, 23.5, 23.0, 21.9, 21.4, 18.5, 17.6, 17.2, 16.2, 10.4
5-3	δ_H 5.21 (s, 1H, 12-H), 4.40 (t, J = 8.2 Hz, 1H, 3-H), 4.05-4.12 (m, 2H), 2.70 (s, 1H), 2.50 (dd, J = 10.8, 4.4 Hz, 1H, H-18), 1.10-2.18 (m, 20H), 1.37-1.55 (m, 4H), 1.07 (s, 3H), 1, 03 (s, 3H), 1.01 (s, 3H), 0.92 (s, H), 0.90 (s, 3H), 0.88 (s, 3H), 0.86 (s, 3H), 0.81 (s, 3H)	δ_C 177.5, 154.0, 138.3, 123.4, 84.3, 74.5, 73.9, 65.3, 55.2, 53.7, 48.4, 47.2, 42.6, 40.5, 39.4, 39.2, 38.5, 37.8, 37.6, 35.9, 34.7, 32.4, 30.2, 28.8, 27.2, 24.5, 24.0, 23.5, 22.6, 22.3, 21.6, 21.5, 18.2, 18.1, 17.5, 16.4, 14.0
5-4	δ_H 5.30 (s, 1H, 12-H), 4.42 (t, J = 7.6 Hz, 1H, 3-H), 4.02-4.10 (m, 2H), 2.74 (s, 1H), 2.56 (dd, J = 12.0, 4.4 Hz, 1H, H-18), 1.05-2.13 (m, 20H), 1.39-1.61 (m, 6H), 1.02 (s, 3H), 0.99 (s, 3H), 0.98 (s, 3H), 0.90 (s, H), 0.89 (s, 3H), 0.88 (s, 3H), 0.86 (s, 3H), 0.84 (s, 3H)	δ_C 177.2, 153.5, 138.0, 124.0, 83.8, 74.2, 73.9, 65.8, 55.6, 53.3, 49.2, 48.5, 44.1, 41.2, 40.2, 39.5, 39.2, 37.2, 36.4, 35.3, 34.2, 30.1, 29.0, 28.4, 27.9, 27.0, 24.3, 23.9, 22.8, 22.5, 22.1, 21.6, 21.4, 18.9, 18.8, 18.2, 16.2, 14.2
5-5	δ_H 5.23 (s, 1H, 12-H), 4.46 (t, J = 7.0 Hz, 1H, 3-H), 4.02-4.08 (m, 2H), 2.70 (s, 1H), 2.56 (dd, J = 12.2, 4.8 Hz, 1H, H-18), 1.12-2.25 (m, 20H), 1.36-1.65 (m, 8H), 1.04 (s, 3H), 0.98 (s, 3H), 0.97 (s, 3H), 0.89 (s, H), 0.87 (s, 3H), 0.85 (s, 3H), 0.80 (s, 3H), 0.78 (s, 3H)	δ_C 177.6, 154.0, 138.5, 123.5, 84.2, 74.5, 74.0, 66.0, 56.2, 54.2, 50.1, 48.3, 45.4, 42.1, 41.8, 40.5, 39.7, 39.2, 38.2, 36.2, 35.1, 31.7, 29.3, 29.2, 28.4, 28.0, 27.5, 24.1, 23.6, 23.4, 22.7, 22.3, 21.5, 21.4, 19.3, 19.2, 18.6, 17.0, 12.7
Ⅰ₁	δ_H 7.79 (s, 1H, triazole-H), 7.05-7.53 (m, 5H), 5.31 (s, 1H, 12-H), 5.20 (d, J = 2.0 Hz, 2H), 4.49 (t, J = 7.2 Hz, 1 H, 3-H), 2.93 (dd, J = 11.0, 4.6 Hz, 1H, H-18), 2.06 (s, 3H), 1.07-2.01 (m, 20H), 0.94-1.05 (m, 2H), 1.02 (s, 3H), 0.91 (s, 3H), 0.90 (s, 3H), 0.89 (s, 3H), 0.86 (s, 3H), 0.85 (s, 3H), 0.82 (s, 3H)	δ_C 179.5, 174.2, 144.7, 134.4, 131.2, 128.4, 128.4, 127.0, 127.0, 122.9, 80.4, 63.1, 55.1, 47.3, 46.7, 46.4, 43.1, 42.0, 39.3, 38.0, 37.7, 36.1, 35.8, 34.1, 32.8, 32.7, 32.2, 30.6, 29.8, 28.0, 27.9, 25.6, 25.6, 23.7, 23.5, 23.5, 21.1, 18.0, 16.9, 16.6, 15.3
Ⅰ₂	δ_H 7.81 (s, 1H, triazole-H), 7.02-7.50 (m, 5H), 5.32 (s, 1H, 12-H), 5.19 (d, J = 3.2 Hz, 2H), 4.50 (t, J = 7.9 Hz, 1H, 3-H), 2.89 (dd, J = 9.2, 4.0 Hz, 1H, H-18), 2.31-2.27 (m, 2H), 1.04-2.05 (m, 20H), 1.22 (s, 3H), 0.94-1.07 (m, 2H), 1.01 (s, 3H), 0.91 (s, 3H), 0.90 (s, 3H), 0.87 (s, 3H), 0.87 (s, 3H), 0.85 (s, 3H), 0.80 (s, 3H)	δ_C 178.5, 171.0, 144.8, 132.3, 132.2, 126.1 126.1, 126.0, 126.0, 122.8, 115.8, 115.6, 80.8, 63.9, 55.1, 53.2, 47.4, 46.7, 46.5, 42.1, 42.0, 39.3, 38.8, 38.1, 37.7, 36.7, 34.1, 33.0, 32.6, 32.2, 28.0, 27.3, 25.7, 23.8, 23.6, 23.5, 23.4, 21.3, 18.1, 16.8, 16.6, 15.2
Ⅰ₃	δ_H 7.84 (s, 1H, triazole-H), 7.05-7.58 (m, 5H), 5.32 (s, 1H, 12-H), 5.23 (d, J = 3.0 Hz, 2H), 4.52 (t, J = 7.2 Hz, 1H, 3-H), 2.85 (dd, J = 8.0, 4.0 Hz, 1H, H-18), 2.35-2.29 (m, 2H), 1.06-2.01 (m, 20H), 1.61-1.68 (m, 2H), 1.60 (m, 2H), 1.23 (s, 3H), 1.15 (s, 3H), 0.95-1.07 (m, 2H), 0.94 (s, 3H), 0.89 (s, 3H), 0.87 (s, 3H), 0.85 (s, 3H), 0.84 (s, 3H), 0.78 (s, 3H)	δ_C 178.4, 170.9, 144.7, 132.7, 132.7, 132.7 132.2, 132.1, 127.7, 125.7, 125.7, 125.4, 125.4, 80.7, 55.0, 47.3, 46.6, 46.3, 42.0, 41.9, 39.2, 38.7, 38.0, 37.7, 37.5, 36.6, 32.8, 32.5, 32.1, 30.6, 27.9, 27.1, 26.8, 25.6, 23.7, 23.4, 23.4, 23.2, 21.2, 18.0, 16.7, 16.5, 15.1
Ⅰ₄	δ_H 7.82 (s, 1H, triazole-H), 7.01-7.50 (m, 5H), 5.32 (s, 1H, 12-H), 5.21 (d, J = 3.4 Hz, 2H), 4.51 (t, J = 7.5 Hz, 1 H, 3-H), 2.83 (dd, J = 8.0, 4.0 Hz, 1H, H-18), 2.33-2.25 (m, 2H), 1.01-2.05 (m, 20H), 1.35-1.80 (m, 4H), 1.25 (s, 3H), 1.16 (s, 3H), 0.92-1.04 (m, 2 H), 1.02 (s, 3H), 0.92 (s, 3H), 0.89 (s, 3H), 0.87 (s, 3H), 0.86 (s, 3H), 0.79 (s, 3H)	δ_C 178.5, 174.3, 144.7, 138.7, 134.9, 132.7, 131.2, 128.0, 128.0, 127.2, 127.2, 122.9, 80.5, 64.6, 55.1, 47.4, 46.7, 46.4, 42.1, 42.1, 42.0, 39.3, 38.6, 38.1, 37.8, 36.8, 34.1, 33.0, 32.6, 32.2, 32.2, 30.7, 28.1, 28.0, 27.3, 23.8, 23.8, 23.5, 23.4, 21.2, 18.1, 16.8, 16.7, 15.3
Ⅰ₅	δ_H 7.87 (s, 1H, triazole-H), 7.03-7.55 (m, 5H), 5.29 (s, 1H, 12-H), 5.19 (d, J = 3.2 Hz, 2H), 4.49 (t, J = 7.2 Hz, 1 H, 3-H), 2.82 (dd, J = 8.4, 4.3 Hz, 1H, H-18), 2.34-2.26 (m, 2H), 1.04-2.08 (m, 20H), 1.26-1.78 (m, 6H), 1.21 (s, 3H), 1.18 (s, 3H), 0.90-1.06 (m, 2 H), 0.96 (s, 3H), 0.93 (s, 3H), 0.89 (s, 3H), 0.86 (s, 3H), 0.85(s, 3H), 0.76 (s, 3H)	δ_C 179.7, 174.4, 144.9, 134.7, 131.4, 128.7, 128.7, 127.2, 127.2, 123.1, 80.6, 63.3, 55.3, 47.6, 46.9, 46.6, 42.2, 42.2, 39.5, 38.3, 37.9, 36.3, 36.0, 34.3, 33.1, 33.0, 32.4, 32.4, 30.8, 30.1, 28.2, 28.1, 27.5, 27.5, 25.8, 25.8, 23.9, 23.7, 23.6, 18.3, 17.1, 16.8, 16.8, 15.5, 9.5
Ⅱ₁	δ_H 7.88 (s, 1H, triazole-H), 7.10-7.55 (m, 5H), 5.43 (s, 1H, 12-H), 4.41 (t, J = 8.0 Hz, 1H, 3-H), 4.25-4.19 (m, 2H), 3.63 (t, J = 7.2 Hz, 2H), 2.58 (dd, J = 12.0, 4.0 Hz, 1H, H-18), 1.01-2.02 (m, 20H), 1.20 (s, 3H), 1.15 (s, 3H), 0.92-1.08 (m, 2H), 0.98 (s, 3H), 0.91 (s, 3H), 0.89 (s, 3H), 0.84 (s, 3H), 0.80(s, 3H), 0.71 (s, 3H)	δ_C 179.5, 174.2, 144.7, 133.5, 131.2, 128.4, 128.4, 127.0, 127.0, 125.9, 125.9, 80.4, 64.4, 55.1, 47.4, 46.7, 46.4, 42.1, 42.0, 42.0, 39.3, 38.0, 37.7, 36.8, 36.1, 35.8, 32.9, 32.8, 32.2, 30.6, 29.9, 28.0, 27.9, 25.6, 23.7, 23.5, 23.4, 21.3, 21.1, 18.6, 16.9, 15.3
Ⅱ₂	δ_H 7.83 (s, 1H, triazole-H), 7.03-7.53 (m, 5H), 5.21 (s, 1H, 12-H), 4.29 (t, J = 8.2 Hz, 1H, 3-H), 4.24-4.17 (m, 2H), 3.60 (t, J = 7.0 Hz, 2H), 2.55 (dd, J = 12.4, 3.5 Hz, 1H, H-18), 1.03-2.06 (m, 20H), 1.72 (m, 2 H), 1.19 (s, 3H), 1.14 (s, 3H), 0.90-1.10 (m, 2H), 0.98 (s, 3H), 0.93 (s, 3H), 0.91 (s, 3H), 0.89 (s, 3H), 0.87 (s, 3H), 0.85 (s, 3H)	δ_C 178.6, 171.2, 144.9, 132.4, 132.4 132.3, 132.3, 126.2, 126.2, 126.1, 125.9, 121.9, 121.9, 121.8, 80.9, 64.0, 55.2, 47.5, 46.9, 46.6, 42.2, 42.1, 39.4, 39.0, 37.8, 36.9, 34.2, 33.1, 32.7, 32.3, 30.8, 28.1, 27.3, 26.7, 25.8, 23.9, 23.6, 23.6, 23.5, 18.2, 16.9, 15.3, 9.33
Ⅱ₃	δ_H 7.86 (s, 1H, triazole-H), 7.09-7.45 (m, 5H), 5.24 (s, 1H, 12-H), 4.23 (t, J = 8.2 Hz, 1 H, 3-H), 4.20-4.10 (m, 2H), 3.58 (t, J = 7.2 Hz, 2H), 2.52 (dd, J = 12.0, 4.5 Hz, 1H, H-18), 1.05-2.10 (m, 20H), 1.32-1.59 (m, 4H), 1.15 (s, 3H), 1.10 (s, 3H), 0.91-1.07 (m, 2H), 0.95 (s, 3H), 0.93 (s, 3H), 0.90 (s, 3H), 0.89 (s, 3H), 0.87 (s, 3H), 0.83 (s, 3H)	δ_C 179.4, 171.0, 144.7, 136.4, 134.2, 131.8, 130.7, 130.0, 130.0, 127.4, 127.4, 122.9, 121.4, 119.7, 80.8, 65.0, 55.2, 47.5, 46.7, 46.4, 42.1, 39.4, 39.4, 38.1, 37.7, 36.9, 36.5, 34.1, 30.7, 29.9, 29.9, 28.0, 27.3, 25.7, 25.7, 24.8, 23.7, 23.6, 23.6, 21.3, 18.2, 17.0, 16.7, 15.4
Ⅱ₄	δ_H 7.84 (s, 1H, triazole-H), 7.13-7.50 (m, 5H), 5.31 (s, 1H, 12-H), 4.26 (t, J = 8.0 Hz, 1H, 3-H), 4.23-4.12 (m, 2H), 3.55 (t, J = 7.2 Hz, 2H), 2.50 (dd, J = 12.0, 4.0 Hz, 1H, H-18), 1.02-2.14 (m, 20H), 1.35-1.69 (m, 6H), 1.12 (s, 3H), 0.95-1.05 (m, 2H), 0.99 (s, 3H), 0.98 (s, 3H), 0.94 (s, 3H), 0.91 (s, 3H), 0.89 (s, 3H), 0.79 (s, 3H), 0.77 (s, 3H)	δ_C 179.6, 174.3, 144.8, 134.6, 131.3, 128.5, 128.5, 127.1, 127.1, 123.0, 80.5, 65.2, 55.2, 47.5, 46.8, 46.5, 42.1, 42.1, 39.4, 39.4, 38.1, 37.8, 36.9, 36.2, 35.9, 33.0, 32.9, 30.7, 30.0, 30.0, 28.1, 27.4, 26.8, 25.7, 25.7, 23.8, 23.6, 23.5, 18.2, 18.2, 17.0, 16.7, 15.4, 15.4, 9.4
Ⅱ₅	δ_H 7.91 (s, 1H, triazole-H), 7.10-7.59 (m, 5H), 5.43 (s, 1H, 12-H), 4.32 (t, J = 8.2 Hz, 1H, 3-H), 4.30-4.16 (m, 2H), 3.58 (t, J = 7.2 Hz, 2H), 2.55 (dd, J = 12.4, 4.2 Hz, 1H, H-18), 1.01-2.07 (m, 20H), 1.28-1.65 (m, 8H), 1.10 (s, 3H), 0.94-1.06 (m, 2H), 0.99 (s, 3H), 0.98 (s, 3H), 0.97 (s, 3H), 0.91 (s, 3H), 0.90 (s, 3H), 0.79 (s, 3H), 0.75 (s, 3H)	δ_C 179.4, 171.1, 144.7, 136.4, 134.2, 131.8, 130.7, 130.7, 130.0, 130.0, 127.4, 127.4, 122.9, 80.9, 66.2, 55.2, 47.5, 46.7, 46.4, 42.1, 42.1, 42.0, 39.4, 38.1, 37.7, 36.9, 36.5, 34.1, 33.0, 32.9, 32.9, 30.7, 30.0, 28.0, 27.3, 25.7, 24.8, 23.7, 21.3, 21.3, 18.2, 17.0, 16.7, 15.4, 15.4, 9.4

Compd.

¹H NMR (600 MHz, CDCl₃)

¹³C NMR (150 MHz, CDCl₃)

3-1

3-2

3-3

3-4

3-5

5-1

5-2

5-3

5-4

5-5

Ⅰ₁

Ⅰ₂

Ⅰ₃

Ⅰ₄

Ⅰ₅

Ⅱ₁

Ⅱ₂

Ⅱ₃

Ⅱ₄

Ⅱ₅

Compd.	IC₅₀/μmol·L^-1		Compd.	IC₅₀/μmol·L^-1
Ⅰ₁	40.22 ± 0.11	37.02 ± 0.07		Ⅱ₁	39.27 ± 0.19	30.18 ± 0.22
Ⅰ₂	31.48 ± 0.10	20.13 ± 0.13	Ⅱ₂	33.32 ± 0.15	21.45 ± 0.08
Ⅰ₃	30.15 ± 0.15	19.21 ± 0.13	Ⅱ₃	23.13 ± 0.20	20.28 ± 0.15
Ⅰ₄	18.22 ± 0.09	12.72 ± 0.15	Ⅱ₄	14.28 ± 0.14	8.46 ± 0.10
Ⅰ₅	38.65 ± 0.16	25.12 ± 0.08	Ⅱ₅	20.68 ± 0.19	19.54 ± 0.17
UA	> 50	> 50	Nilotinib	15.40 ± 0.10	10.15 ± 0.08

Compd.

IC₅₀/μmol·L^-1

Compd.

IC₅₀/μmol·L^-1

MCF-7

SGC-7901

MCF-7

SGC-7901

Ⅰ₁

40.22 ± 0.11

37.02 ± 0.07

Ⅱ₁

39.27 ± 0.19

30.18 ± 0.22

Ⅰ₂

31.48 ± 0.10

20.13 ± 0.13

Ⅱ₂

33.32 ± 0.15

21.45 ± 0.08

Ⅰ₃

30.15 ± 0.15

19.21 ± 0.13

Ⅱ₃

23.13 ± 0.20

20.28 ± 0.15

Ⅰ₄

18.22 ± 0.09

12.72 ± 0.15

Ⅱ₄

14.28 ± 0.14

8.46 ± 0.10

Ⅰ₅

38.65 ± 0.16

25.12 ± 0.08

Ⅱ₅

20.68 ± 0.19

19.54 ± 0.17

> 50

Nilotinib

15.40 ± 0.10

10.15 ± 0.08

Compd.	IC₅₀/μmol·L^-1		Compd.	IC₅₀/μmol·L^-1
Ⅰ₁	40.22 ± 0.11	37.02 ± 0.07		Ⅱ₁	39.27 ± 0.19	30.18 ± 0.22
Ⅰ₂	31.48 ± 0.10	20.13 ± 0.13	Ⅱ₂	33.32 ± 0.15	21.45 ± 0.08
Ⅰ₃	30.15 ± 0.15	19.21 ± 0.13	Ⅱ₃	23.13 ± 0.20	20.28 ± 0.15
Ⅰ₄	18.22 ± 0.09	12.72 ± 0.15	Ⅱ₄	14.28 ± 0.14	8.46 ± 0.10
Ⅰ₅	38.65 ± 0.16	25.12 ± 0.08	Ⅱ₅	20.68 ± 0.19	19.54 ± 0.17
UA	> 50	> 50	Nilotinib	15.40 ± 0.10	10.15 ± 0.08

Compd.

IC₅₀/μmol·L^-1

Compd.

IC₅₀/μmol·L^-1

MCF-7

SGC-7901

MCF-7

SGC-7901

Ⅰ₁

40.22 ± 0.11

37.02 ± 0.07

Ⅱ₁

39.27 ± 0.19

30.18 ± 0.22

Ⅰ₂

31.48 ± 0.10

20.13 ± 0.13

Ⅱ₂

33.32 ± 0.15

21.45 ± 0.08

Ⅰ₃

30.15 ± 0.15

19.21 ± 0.13

Ⅱ₃

23.13 ± 0.20

20.28 ± 0.15

Ⅰ₄

18.22 ± 0.09

12.72 ± 0.15

Ⅱ₄

14.28 ± 0.14

8.46 ± 0.10

Ⅰ₅

38.65 ± 0.16

25.12 ± 0.08

Ⅱ₅

20.68 ± 0.19

19.54 ± 0.17

> 50

Nilotinib

15.40 ± 0.10

10.15 ± 0.08

Compd.	Docking score		Compd.	Docking score
Ⅰ₁	-154.697		Ⅱ₁	-155.338
Ⅰ₂	-162.983	Ⅱ₂	-160.921
Ⅰ₃	-162.793	Ⅱ₃	-161.544
Ⅰ₄	-167.873	Ⅱ₄	-172.941
Ⅰ₅	-161.868	Ⅱ₅	-165.844
Nilotinib	-170.459	UA	-120.341

Compd.

Docking score

Compd.

Docking score

Ⅰ₁

-154.697

Ⅱ₁

-155.338

Ⅰ₂

-162.983

Ⅱ₂

-160.921

Ⅰ₃

-162.793

Ⅱ₃

-161.544

Ⅰ₄

-167.873

Ⅱ₄

-172.941

Ⅰ₅

-161.868

Ⅱ₅

-165.844

Nilotinib

-170.459

-120.341

Compd.	Docking score		Compd.	Docking score
Ⅰ₁	-154.697		Ⅱ₁	-155.338
Ⅰ₂	-162.983	Ⅱ₂	-160.921
Ⅰ₃	-162.793	Ⅱ₃	-161.544
Ⅰ₄	-167.873	Ⅱ₄	-172.941
Ⅰ₅	-161.868	Ⅱ₅	-165.844
Nilotinib	-170.459	UA	-120.341

Compd.

Docking score

Compd.

Docking score

Ⅰ₁

-154.697

Ⅱ₁

-155.338

Ⅰ₂

-162.983

Ⅱ₂

-160.921

Ⅰ₃

-162.793

Ⅱ₃

-161.544

Ⅰ₄

-167.873

Ⅱ₄

-172.941

Ⅰ₅

-161.868

Ⅱ₅

-165.844

Nilotinib

-170.459

-120.341

熊果酸-三氮唑衍生物的合成及抗肿瘤活性研究

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蒯振彧 ¹^,^* , 孟艳秋 ²

药学学报 | 研究论文 2025,60(1): 172-178

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药学学报 | 研究论文 2025, 60(1): 172-178

熊果酸-三氮唑衍生物的合成及抗肿瘤活性研究

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蒯振彧¹^,^*, 孟艳秋²

作者信息

1.马鞍山职业技术学院药学教研室, 安徽马鞍山 243031

2.沈阳化工大学制药与生物工程学院, 辽宁沈阳 110142

通讯作者:

^*蒯振彧, Tel: 86-153-57963077, E-mail: zhenyukuai999@163.com

Synthesis and anti-tumor activity of ursolic acid-triazole derivatives

Zhen-yu KUAI¹^,^*, Yan-qiu MENG²

Affiliations

1. Department of Pharmacy Teaching and Research, Maanshan Technical College, Maanshan 243031, China

2. Department of Pharmaceutical Engineering, Shenyang University of Chemical Technology, Shenyang 110142, China

出版时间: 2025-01-12 doi: 10.16438/j.0513-4870.2024-0698

文章导航

摘要

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以熊果酸为母体, 对其C-3和C-28位进行结构修饰, 引入1, 2, 3-三氮唑, 合成了10个新型熊果酸衍生物, 结构经MS、¹H NMR和¹³C NMR确证。通过MTT法, 选用高表达人癌细胞(乳腺癌MCF-7和胃癌SGC-7901细胞) 对化合物进行初步体外抗肿瘤活性筛选, 结果表明, 所有化合物对MCF-7和SGC-7901肿瘤细胞的抑制活性均明显高于熊果酸。其中化合物Ⅱ₄对MCF-7和SGC-7901细胞具有较强的抗肿瘤作用, 活性与阳性对照药尼洛替尼相当, 分子对接也显示其与c-Kit具有较高的结合能力, 值得进一步研究。

关键词

熊果酸 / 分子对接 / c-Kit / 结构修饰 / 抗肿瘤活性

Abstract

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Ten ursolic acid derivatives were designed from the lead compound ursolic acid by introducing 1, 2, 3-triazole at C-3 and C-28. The target compounds were synthesized and characterized by ¹H NMR and ¹³C NMR. MTT assay was used to study the antitumor activity of these compounds in human cancer cells with high expression (MCF-7 and SGC-7901). The results showed that the antitumor activity of all compounds on MCF-7 and SGC-7901 tumor cells was significantly higher than that of ursolic acid. The compound Ⅱ₄ exhibited significant antitumor activity which was equivalent to the positive control drug nilotinib, molecular docking showed that the compound Ⅱ₄ have high binding ability with c-Kit, which deserves further research.

Key words

ursolic acid / molecular docking / c-Kit / structural modification / anti-tumor activity

引用本文

蒯振彧, 孟艳秋. 熊果酸-三氮唑衍生物的合成及抗肿瘤活性研究. 药学学报, 2025 , 60 (1) : 172 -178 . DOI: 10.16438/j.0513-4870.2024-0698

Zhen-yu KUAI, Yan-qiu MENG. Synthesis and anti-tumor activity of ursolic acid-triazole derivatives[J]. Acta Pharmaceutica Sinica, 2025 , 60 (1) : 172 -178 . DOI: 10.16438/j.0513-4870.2024-0698

正文

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熊果酸(ursolic acid, UA) 别名乌索酸, 是一种天然的乌苏烷型五环三萜类化合物(图 1)。熊果酸具有抗菌、抗病毒、抗肿瘤、抗氧化、治疗糖尿病、保肝等药理活性^[1-4]。熊果酸已被公认具有良好的抗肿瘤活性和广泛的抗肿瘤途径^[5], 如诱导肿瘤细胞分化及凋亡^[6], 抑制肿瘤细胞生成和增殖等^[7], 为了改善其水溶性差, 生物利用度低的缺点, 研究人员对熊果酸结构进行修饰, 多集中在C-3位羟基和C-28位羧基^[8], 得到的衍生物活性及药动学性质都得到增强和改善。

在药物及天然产物的衍生物设计中, 拼合原理^[9]的应用尤具潜力, 即在同一分子中将两种或多种药物的结构或药效团进行拼合, 达到提高相关生物活性或降低毒副作用的效果。含氮唑环作为常见的药物结构活性基团^[10], 具有抗炎、抗菌、抗肿瘤等作用^[11], 独特结构可与目标靶点结合形成范德华力或氢键等偶极作用, 且化学性质稳定, 作为极性基团也可提高化合物的溶解度从而改善生物利用度^[12]。三氮唑基团是唑类基团中热点药效团^[13], 具有1, 2, 3-三氮唑和1, 2, 4-三氮唑两种异构体形式, 其中前者的结构具有较强的偶极矩, 作为可能的氢键供给体, 在与靶点结合亲和力方面表现更优^[14]。Kim等^[15]通过将神经酰胺的酰胺键替换为1, 2, 3-三氮唑, 设计出了具有更高细胞毒性的新型化合物, 一些基于1, 2, 3-三氮唑结构的衍生物也进入临床评估其在癌症治疗中的潜在用途^[16]。

结合课题组前期利用分子对接技术及化学合成方法对五环三萜化合物的设计和结构修饰研究^[17-19], 拼合含氮杂环可提高化合物与靶点蛋白的结合能力, 细胞实验显示相较于母体, 获得了更显著的抗肿瘤活性。本研究在前期工作基础上, 选择在多个恶性肿瘤中具有高表达的Ⅲ型受体酪氨酸激酶(c-Kit) 作为靶点蛋白^[20], 将设计出的熊果酸衍生物与受体c-Kit晶体结构(PDB: 1T46) 进行分子对接^[21], 以UA为起始原料, 对其C-3位和C-28位分别引入酯键和1, 2, 3-三氮唑结构, 并通过连接碳链的长短初步探究构效关系(图 2)。

为了验证设计合理性, 使用分子对接软件Molegro virtual docker (MVD) 评估目标化合物与c-Kit靶蛋白的结合能力, 目标化合物与蛋白结合均优于母体, 可进行下一步合成。

结果

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1 化学合成

如合成路线1所示, 以UA为母体, 将1, 2, 3-三氮唑结构分别引入其C-3和C-28位, 设计并合成了10种UA衍生物, 目标化合物Ⅰ₁~Ⅰ₅、Ⅱ₁~Ⅱ₅的收率、熔点、质谱数据列于表 1, 中间体3-1~3-5、5-1~5-5及目标化合物Ⅰ₁~Ⅰ₅、Ⅱ₁~Ⅱ₅的¹H NMR、¹³C NMR数据列于表 2。

2 生物活性评价

以尼洛替尼^[22]为阳性对照, 采用四甲基偶氮唑盐法(MTT法), 在癌细胞(人乳腺癌细胞MCF-7和人胃癌细胞SGC-7901) 中检测熊果酸及其衍生物的抗肿瘤活性, 实验结果见表 3, 化合物Ⅱ₄对MCF-7和SGC-7901细胞的IC₅₀值分别为14.28和8.46 μmol·L^-1, 接近阳性对照药尼洛替尼, 具有较强的抑制活性。

3 分子对接

选择c-Kit抑制剂尼洛替尼和母体熊果酸作对照, 进行分子对接研究, 评分绝对值越大, 结合亲和力越强, 结果如表 4所示。衍生物结构中拼合1, 2, 3-三氮唑结构, 对接得分明显高于母体, 与尼罗替尼相近, 其中化合物Ⅱ₄对接得分最高。为了明确化合物Ⅱ₄与c-Kit的相互作用情况, 利用Discovery Studio 2019 Client软件, 分析结合位点可能的结合方式(图 3)。化合物Ⅱ₄能够进入蛋白的活性口袋, 并与c-Kit蛋白的多个氨基酸残基形成氢键、疏水等相互作用。对比c-Kit抑制剂尼洛替尼的对接结果, 发现二者都与氨基酸残基Asp810形成氢键, 且化合物Ⅱ₄连接氢键的为C-28位拼合的1, 2, 3-三氮唑结构, 有文献^[23]报道, c-Kit抑制剂能与Asp810产生氢键作用, 推测氨基酸残基Asp810是c-Kit蛋白与抑制剂结合的关键氨基酸。初步判断化合物Ⅱ₄和尼洛替尼与靶蛋白具有相同的结合位点。

4 结论

以UA为母体, 设计并合成了10种UA衍生物, 结构经MS和NMR表征。体外细胞实验表明, 化合物对癌细胞MCF-7和SGC-7901具有抑制作用, 初步的构效关系研究表明, C-3位引入1, 2, 3-三氮唑结构的化合物活性优于C-28位, 且引入碳链的长短会影响衍生物活性, 两类化合物酯键碳数目为5时, 活性最优, 其中化合物Ⅱ₄对c-Kit的抑制活性与阳性对照药尼洛替尼相当, 分子对接结果显示化合物中的三氮唑作为酰胺基团的生物电子等排体, 具有提高化合物亲和力的作用, 分析化合物Ⅱ₄和尼洛替尼均与Asp810氨基酸残基形成氢键, 具有相同的结合位点。总体而言, UA结构中引入三氮唑结构, 有利于增强其抗肿瘤活性。这对熊果酸进一步结构修饰及优化具有一定的参考意义。

实验部分

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1 主要仪器与试剂

Büchi B-540熔点测定仪(瑞士BUCHI公司); BrukerARX-600型核磁共振分析仪(瑞士Bruker公司), CDCl₃为溶剂, TMS为内标; 热电-菲尼根LCQ型质谱仪(美国San Francisco公司); SPECTRA MAX plus型酶标仪(美国MDC公司); XDS-1B倒置显微镜(重庆留辉科技有限公司); TGL-16B低速台式离心机(上海安亭科学仪器厂)。熊果酸(质量分数 > 98%) 购于中山市远洋生物医药技术有限公司; 显色剂为10% (体积分数) 硫酸-乙醇溶液; 活性测试所用的RPMI-1640培养基(含10%胎牛血清, 100 μmol·L^-1青霉素, 100 μmol·L^-1链霉素)、溴化四偶氮唑盐(MTT) 购于南京绿合生化科技有限公司。所有试剂均为分析纯或化学纯。

2 化学合成

2.1 3β-乙酰氧基-乌苏烷型-12-烯-28-羧酸(2-1) 的合成

参照课题组前期合成方法^[17-19], 向起始原料UA (500 mg, 1.10 mmol) 的THF (50 mL) 溶液中缓慢滴加乙酸酐(0.565 mL, 6.00 mmol), 室温搅拌, 加入DMAP (10 mg, 0.09 mmol), 室温搅拌4 h, TLC跟踪反应完成。用饱和氯化钠溶液(20 mL) 洗涤, 后用乙酸乙酯(10 mL) 萃取3次, 有机相合并后经无水硫酸钠干燥, 过滤, 减压蒸除溶剂, 得到的粗产物经硅胶柱层析纯化, 洗脱液[石油醚∶乙酸乙酯= 10∶1 (v/v)], 得到450 mg白色固体2-1, 产率82%, m.p. 184.6~185.7 ℃。

2.2 3β-乙酰氧基-乌苏烷型-12-烯-28-羧酸炔丙酯(3-1) 的合成

将化合物2-1 (400 mg, 0.80 mmol) 加入DMF (20 mL) 中, 再依次加入炔丙溴(350 mg, 2.32 mmol) 和碳酸钾(110 mg, 0.80 mmol), 控温60 ℃反应8 h后降至室温, 用饱和氯化钠溶液(20 mL) 洗涤, 后用二氯甲烷(10 mL) 萃取3次, 有机相合并后经无水硫酸钠干燥, 过滤, 减压蒸除溶剂, 得到的粗产物经硅胶柱层析纯化, 洗脱液[石油醚∶乙酸乙酯= 10∶1 (v/v)], 得到343 mg类白色固体3-1, 产率80%, m.p. 191.6~193.1 ℃。

2.3 3β-羟基-乌苏烷型-12-烯-28-羧酸乙酯(4-1) 的合成

将起始原料UA (500 mg, 1.10 mmol)、碳酸钾(300 mg, 2.17 mmol) 加入DMF (20 mL) 中, 缓慢滴加溴乙烷(0.24 mL, 5.02 mmol), 室温搅拌5 h, TLC跟踪反应完成。减压蒸发除去溶剂, 用饱和氯化钠溶液(20 mL) 洗涤, 后用乙酸乙酯(10 mL) 萃取3次, 合并有机相, 经无水硫酸钠干燥, 过滤, 减压蒸除溶剂, 得到的粗产物经硅胶柱层析纯化, 洗脱液[石油醚∶乙酸乙酯= 15∶1 (v/v)], 得到353 mg白色固体4-1, 产率66%, m.p. 177.2~178.7 ℃。

2.4 3β-(3-炔基丁酰氧)-乌苏烷型-12-烯-28-羧酸乙酯(5-1) 的合成

将3-丁炔酸(84 mg, 1.00 mmol) 溶解于二氯甲烷(10 mL) 中, 搅拌加入DCC (206 mg, 1.00 mmol), 10 mn后缓慢加入DMAP (10 mg, 0.09 mmol) 和已溶解于二氯甲烷(20 mL) 中的化合物4-1 (480 mg, 1.00 mmol), 室温搅拌3 h, TLC跟踪反应完成。用饱和氯化钠溶液(20 mL) 洗涤, 后用二氯甲烷(10 mL) 萃取3次, 有机相合并后经无水硫酸钠干燥, 过滤, 减压蒸除溶剂, 得到的粗产物经硅胶柱层析纯化, 洗脱液[石油醚∶乙酸乙酯= 8∶2 (v/v)], 得到321 mg白色固体5-1, 产率58%, m.p. 173.2~173.9 ℃。

2.5 3β-乙酰氧基-乌苏烷型-12-烯-28-酸(1-苯基三氮唑-4-)甲酯(Ⅰ₁) 的合成

将化合物3-1 (376 mg, 0.70 mmol) 加入到无水乙醇(10 mL) 中, 再依次加入叠氮苯(143 mg, 1.20 mmol)、抗坏血酸钠(36 mg, 0.18 mmol) 和五水合硫酸铜(100 mg, 0.40 mmol) 室温搅拌4 h, TLC跟踪反应完成。减压蒸发除去溶剂, 用饱和氯化钠溶液(20 mL) 洗涤, 后用乙酸乙酯(10 mL) 萃取3次, 合并有机相, 经无水硫酸钠干燥, 过滤, 减压蒸除溶剂, 得到的粗产物经硅胶柱层析纯化, 洗脱液[石油醚∶乙酸乙酯= 20∶1 (v/v)], 得到白色固体Ⅰ₁。

2.6 3β-丙酰氧基-乌苏烷型-12-烯-28-酸(1-苯基三氮唑-4-)甲酯(Ⅰ₂) 的合成

按照制备Ⅰ₁的方法, 由母体UA (1.10 mmol) 与丙酸酐(6.00 mmol) 反应。最终粗品经硅胶柱色谱纯化, 洗脱液[石油醚∶乙酸乙酯= 20∶1 (v/v)], 得到白色固体Ⅰ₂。

2.7 3β-丁酰氧基-乌苏烷型-12-烯-28-酸(1-苯基三氮唑-4-)甲酯(Ⅰ₃) 的合成

按照制备Ⅰ₁的方法, 由母体UA (1.10 mmol) 与丁酸酐(6.00 mmol) 反应。最终粗品经硅胶柱色谱纯化, 洗脱液[石油醚∶乙酸乙酯= 20∶1 (v/v)], 得到白色固体Ⅰ₃。

2.8 3β-戊酰氧基-乌苏烷型-12-烯-28-酸(1-苯基三氮唑-4-)甲酯(Ⅰ₄) 的合成

按照制备Ⅰ₁的方法, 由母体UA (1.10 mmol) 与戊酸酐(6.00 mmol) 反应。最终粗品经硅胶柱色谱纯化, 洗脱液[石油醚∶乙酸乙酯= 20∶1 (v/v)], 得到白色固体Ⅰ₄。

2.9 3β-己酰氧基-乌苏烷型-12-烯-28-酸(1-苯基三氮唑-4-)甲酯(Ⅰ₅) 的合成

按照制备Ⅰ₁的方法, 由母体UA (1.10 mmol) 与己酸酐(6.00 mmol) 反应。最终粗品经硅胶柱色谱纯化, 洗脱液[石油醚∶乙酸乙酯= 20∶1 (v/v)], 得到白色固体Ⅰ₅。

2.10 3β-(1-苯基三氮唑-4-)乙酰氧基-乌苏烷型-12-烯-28-羧酸乙酯(Ⅱ₁) 的合成

按照制备Ⅰ₁的方法, 将化合物5-1 (385 mg, 0.70 mmol) 加入反应, 得到的粗产物经硅胶柱层析纯化, 洗脱液[石油醚∶乙酸乙酯= 15∶1 (v/v)], 得到白色固体Ⅱ₁。

2.11 3β-(1-苯基三氮唑-4-)乙酰氧基-乌苏烷型-12-烯-28-羧酸丙酯(Ⅱ₂) 的合成

按照制备4-1和5-1的方法, UA与溴丙烷反应得到5-2后, 再按照制备Ⅰ₁的方法, 将化合物5-2 (0.70 mmol) 加入反应, 得到的粗产物经硅胶柱层析纯化, 洗脱液[石油醚∶乙酸乙酯= 15∶1 (v/v)], 得到白色固体Ⅱ₂。

2.12 3β-(1-苯基三氮唑-4-)乙酰氧基-乌苏烷型-12-烯-28-羧酸丁酯(Ⅱ₃) 的合成

按照制备4-1和5-1的方法, UA与溴丁烷反应得到5-3后, 再按照制备Ⅰ₁的方法, 将化合物5-3 (0.70 mmol) 加入反应, 得到的粗产物经硅胶柱层析纯化, 洗脱液[石油醚∶乙酸乙酯= 15∶1 (v/v)], 得到白色固体Ⅱ₃。

2.13 3β-(1-苯基三氮唑-4-)乙酰氧基-乌苏烷型-12-烯-28-羧酸戊酯(Ⅱ₄) 的合成

按照制备4-1和5-1的方法, UA与溴戊烷反应得到5-4后, 再按照制备Ⅰ₁的方法, 将化合物5-4 (0.70 mmol) 加入反应, 得到的粗产物经硅胶柱层析纯化, 洗脱液[石油醚∶乙酸乙酯= 15∶1 (v/v)], 得到白色固体Ⅱ₄。

2.14 3β-(1-苯基三氮唑-4-)乙酰氧基-乌苏烷型-12-烯-28-羧酸己酯(Ⅱ₅) 的合成

按照制备4-1和5-1的方法, UA与溴己烷反应得到5-5后, 再按照制备Ⅰ₁的方法, 将化合物5-5 (0.70 mmol) 加入反应, 得到的粗产物经硅胶柱层析纯化, 洗脱液[石油醚∶乙酸乙酯= 15∶1 (v/v)], 得到白色固体Ⅱ₅。

3 生物活性试验

采用MTT法, 对数生长期的肿瘤细胞用胰蛋白酶消化, 然后用RPMI-1640培养基制备每毫升2.5×10⁴个单细胞悬液。将对数生长期的肿瘤细胞置于96孔培养板中, 每孔100 µL, 置于37 ℃恒温箱(5% CO₂) 中培养24 h后, 给药组加入配置成0.1、1、10、20、50 μmol·L^-1的测试物, 并设置3个平行板, 加入化合物后轻摇板, 孵育72 h, 每孔加入20 μL MTT溶液(5 mg·mL^-1), 培养4 h, 去除上清后加入DMSO, 使形成的紫色甲酰胺晶体溶解。测定490 nm下的光密度(OD) 值, 使用GraphPad 5.0软件确定IC₅₀值。该实验独立重复3次。

作者贡献: 蒯振彧负责设计研究方案, 实施研究过程, 采集数据整理, 文章撰写及修订; 孟艳秋对本文提供指导性支持。

利益冲突: 本文所有作者声明不存在利益冲突关系。

基金

收起

安徽省高校自然科学研究项目(2024AH051790)
安徽省高校自然科学研究项目(KJ2021A1340)
国家自然科学基金资助项目(82473787)

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doi: 10.16438/j.0513-4870.2024-0698

接收时间：2024-07-22
首发时间：2025-11-07
出版时间：2025-01-12

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收稿日期：2024-07-22
修回日期：2024-09-09

基金

安徽省高校自然科学研究项目(2024AH051790)

安徽省高校自然科学研究项目(KJ2021A1340)

国家自然科学基金资助项目(82473787)

作者信息

1.马鞍山职业技术学院药学教研室, 安徽马鞍山 243031

2.沈阳化工大学制药与生物工程学院, 辽宁沈阳 110142

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^*蒯振彧, Tel: 86-153-57963077, E-mail: zhenyukuai999@163.com

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2种不同金属材料的力学参数

科 Family	属数 Number of genus	种数 Number of species	占总种数比例 Percentage of total species (%)	属 Genus	种数 Number of species	占总种数比例 Percentage of total species (%)
鹅膏菌科Amanitaceae	2	11	5.26	鹅膏菌属 Amanita	10	4.78
小菇科 Mycenaceae	2	12	5.74	丝盖伞属 Inocybe	5	2.39
多孔菌科 Polyporaceae	8	14	6.70	蜡蘑属 Laccaria	5	2.39
红菇科 Russulaceae	3	23	11.00	小皮伞属 Marasmius	6	2.87
				小菇属 Mycena	11	5.26
				光柄菇属 Pluteus	5	2.39
				红菇属 Russula	17	8.13
				栓菌属 Trametes	5	2.39

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Compd.	Yield/%	Melting/℃	ESI-MS m/z [M+H]⁺
Ⅰ₁	50.4	176.3-177.7	656.29
Ⅰ₂	49.2	175.0-176.3	671.24
Ⅰ₃	55.6	171.4-172.7	684.51
Ⅰ₄	57.5	172.8-174.2	698.62
Ⅰ₅	58.1	168.5-170.0	712.52
Ⅱ₁	48.2	169.5-170.4	670.52
Ⅱ₂	50.6	170.2-171.1	684.37
Ⅱ₃	47.2	167.8-168.5	698.79
Ⅱ₄	50.8	165.2-166.3	712.64
Ⅱ₅	46.1	162.7-163.8	726.82

Compd.

Yield/%

Melting/℃

ESI-MS m/z [M+H]⁺

Ⅰ₁

50.4

176.3-177.7

656.29

Ⅰ₂

49.2

175.0-176.3

671.24

Ⅰ₃

55.6

171.4-172.7

684.51

Ⅰ₄

57.5

172.8-174.2

698.62

Ⅰ₅

58.1

168.5-170.0

712.52

Ⅱ₁

48.2

169.5-170.4

670.52

Ⅱ₂

50.6

170.2-171.1

684.37

Ⅱ₃

47.2

167.8-168.5

698.79

Ⅱ₄

50.8

165.2-166.3

712.64

Ⅱ₅

46.1

162.7-163.8

726.82

Compd.	¹H NMR (600 MHz, CDCl₃)	¹³C NMR (150 MHz, CDCl₃)
3-1	δ_H 5.28 (s, 1H, 12-H), 4.40 (t, J = 7.0 Hz, 1H, 3-H), 2.89 (dd, J = 11.2, 4.2 Hz, 1H, H-18), 2.97(s, 1H), 2.06 (s, 3H), 1.03-2.04 (m, 20H), 0.90-1.07 (m, 2 H), 1.05 (s, 3H), 0.93 (s, 3H), 0.91 (s, 3H), 0.90 (s, 3H), 0.85 (s, 3H), 0.82 (s, 3H), 0.79 (s, 3H)	δ_C 179.5, 172.6, 143.3, 123.8, 83.1, 81.0, 55.2, 52.0, 47.6, 46.4, 46.1, 43.0, 42.4, 40.2, 39.8, 38.2, 37.8, 35.7, 32.4, 31.5, 30.4, 29.2, 27.2, 25.0, 24.3, 23.8, 23.5, 23.4, 22.0, 19.6, 17.3, 16.7, 16.2
3-2	δ_H 5.30 (s, 1H, 12-H), 4.45 (t, J = 7.2 Hz, 1H, 3-H), 2.85 (dd, J = 12.0, 4.8 Hz, 1H, H-18), 2.95 (s, 1H), 2.27-2.30 (m, 2H), 1.01-2.07 (m, 20H), 0.97-1.09 (m, 2 H), 1.19 (s, 3H), 1.02 (s, 3H), 0.92 (s, 3H), 0.91 (s, 3H), 0.90 (s, 3H), 0.87 (s, 3H), 0.84 (s, 3H), 0.81 (s, 3H)	δ_C 179.0, 174.3, 143.9, 124.3, 82.4, 80.3, 55.6, 52.4, 47.3, 46.7, 46.5, 42.7, 42.1, 39.7, 39.1, 37.9, 37.6, 36.2, 32.9, 31.2, 29.4, 28.3, 26.7, 24.7, 24.0, 23.5, 23.4, 23.1, 21.6, 19.0, 17.7, 16.9, 16.4, 9.7
3-3	δ_H 5.27 (s, 1H, 12-H), 4.42 (t, J = 7.2 Hz, 1H, 3-H), 2.85 (dd, J = 10.6, 4.0 Hz, 1H, H-18), 2.98 (s, 1H), 1.83-2.35 (m, 4H), 1.05-2.02 (m, 20H), 0.95-1.12 (m, 2 H), 1.05 (s, 3H), 1.02 (s, 3H), 1.00 (s, 3H), 0.95 (s, 3H), 0.92 (s, 3H), 0.88 (s, 3H), 0.87 (s, 3H), 0.84 (s, 3H)	δ_C 179.7, 174.0, 140.1, 123.1, 83.5, 81.1, 55.0, 52.1, 47.5, 46.2, 43.1, 42.3, 40.1, 39.2, 38.7, 38.5, 37.2, 37.0, 36.2, 36.0, 33.2, 30.8, 28.8, 28.0, 27.2, 24.2, 23.8, 23.5, 23.0, 22.6, 21.6, 18.5, 18.1, 16.1, 14.0
3-4	δ_H 5.32 (s, 1H, 12-H), 4.38 (t, J = 7.0 Hz, 1 H, 3-H), 2.85 (dd, J = 12.2, 4.6 Hz, 1H, H-18), 2.92 (s, 1H), 1.40-2.32 (m, 6H), 1.10-2.13 (m, 20H), 0.97-1.15 (m, 2H), 1.08 (s, 3H), 1.05 (s, 3H), 0.97 (s, 3H), 0.93 (s, 3H), 0.92 (s, 3H), 0.89 (s, 3H), 0.87 (s, 3H), 0.82 (s, 3H)	δ_C 180.2, 173.2, 139.5, 123.4, 83.7, 80.6, 55.6, 52.4, 47.1, 46.6, 42.8, 41.7, 41.2, 39.9, 38.2, 37.4, 37.0, 36.8, 36.4, 36.2, 34.2, 31.2, 29.3, 28.5, 26.9, 24.5, 23.8, 23.6, 23.1, 22.3, 21.2, 18.4, 17.7, 16.9, 16.6, 13.5
3-5	δ_H 5.26 (s, 1H, 12-H), 4.38 (t, J = 7.0 Hz, 1H, 3-H), 2.85 (dd, J = 10.4, 4.0 Hz, 1H, H-18), 2.95 (s, 1H), 1.25-2.36 (m, 8H), 1.12-2.21 (m, 20H), 0.94-1.14 (m, 2H), 1.10 (s, 3H), 1.04 (s, 3H), 1.02 (s, 3H), 0.97 (s, 3H), 0.93 (s, 3H), 0.92 (s, 3H), 0.88 (s, 3H), 0.87 (s, 3H)	δ_C 180.0, 173.5, 138.2, 123.0, 84.0, 81.2, 55.2, 52.0, 47.7, 46.2, 43.1, 42.0, 40.6, 39.7, 39.2, 37.8, 37.5, 37.0, 36.4, 36.0, 33.7, 31.6, 31.2, 29.0, 28.9, 26.3, 24.1, 24.0, 23.8, 23.4, 22.1, 21.6, 18.1, 17.2, 16.7, 16.0, 14.2
5-1	δ_H 5.32 (s, 1H, 12-H), 4.45 (t, J = 7.4 Hz, 1H, 3-H), 4.15-4.21 (m, 2H), 2.82 (s, 1H), 2.55 (dd, J = 10.8, 4.0 Hz, 1H, H-18), 1.10-2.11 (m, 20H), 1.23 (s, 3H), 1.10 (s, 3H), 1, 02 (s, 3H), 0.90 (s, 3H), 0.89 (s, 3H), 0.88 (s, 3H), 0.85 (s, 3H), 0.82 (s, 3H)	δ_C 177.5, 160.2, 140.2, 123.3, 84.1, 75.1, 74.6, 61.3, 55.7, 52.8, 48.6, 47.1, 42.8, 40.5, 39.5, 39.0, 38.9, 37.8, 37.5, 37.0, 33.2, 30.7, 29.1, 26.5, 24.6, 24.0, 23.8, 23.6, 23.2, 21.4, 18.9, 17.6, 17.0, 16.5, 15.1
5-2	δ_H 5.28 (s, 1H, 12-H), 4.42 (t, J = 8.0 Hz, 1H, 3-H), 4.10-4.20 (m, 2H), 2.74 (s, 1H), 2.52 (dd, J = 9.4, 4.2 Hz, 1H, H-18), 1.06-2.14 (m, 20H), 1.68-1.74 (m, 2H), 1.10 (s, 3H), 1, 02 (s, 3H), 0.98 (s, 3H), 0.90 (s, H), 0.88 (s, 3H), 0.86 (s, 3H), 0.85 (s, 3H), 0.83 (s, 3H)	δ_C 177.0, 155.6, 139.1, 123.0, 83.5, 74.9, 73.8, 65.7, 55.7, 53.2, 48.6, 47.5, 42.2, 40.2, 39.1, 38.7, 38.5, 37.6, 37.3, 36.8, 33.5, 30.5, 29.4, 26.0, 24.3, 23.9, 23.7, 23.5, 23.0, 21.9, 21.4, 18.5, 17.6, 17.2, 16.2, 10.4
5-3	δ_H 5.21 (s, 1H, 12-H), 4.40 (t, J = 8.2 Hz, 1H, 3-H), 4.05-4.12 (m, 2H), 2.70 (s, 1H), 2.50 (dd, J = 10.8, 4.4 Hz, 1H, H-18), 1.10-2.18 (m, 20H), 1.37-1.55 (m, 4H), 1.07 (s, 3H), 1, 03 (s, 3H), 1.01 (s, 3H), 0.92 (s, H), 0.90 (s, 3H), 0.88 (s, 3H), 0.86 (s, 3H), 0.81 (s, 3H)	δ_C 177.5, 154.0, 138.3, 123.4, 84.3, 74.5, 73.9, 65.3, 55.2, 53.7, 48.4, 47.2, 42.6, 40.5, 39.4, 39.2, 38.5, 37.8, 37.6, 35.9, 34.7, 32.4, 30.2, 28.8, 27.2, 24.5, 24.0, 23.5, 22.6, 22.3, 21.6, 21.5, 18.2, 18.1, 17.5, 16.4, 14.0
5-4	δ_H 5.30 (s, 1H, 12-H), 4.42 (t, J = 7.6 Hz, 1H, 3-H), 4.02-4.10 (m, 2H), 2.74 (s, 1H), 2.56 (dd, J = 12.0, 4.4 Hz, 1H, H-18), 1.05-2.13 (m, 20H), 1.39-1.61 (m, 6H), 1.02 (s, 3H), 0.99 (s, 3H), 0.98 (s, 3H), 0.90 (s, H), 0.89 (s, 3H), 0.88 (s, 3H), 0.86 (s, 3H), 0.84 (s, 3H)	δ_C 177.2, 153.5, 138.0, 124.0, 83.8, 74.2, 73.9, 65.8, 55.6, 53.3, 49.2, 48.5, 44.1, 41.2, 40.2, 39.5, 39.2, 37.2, 36.4, 35.3, 34.2, 30.1, 29.0, 28.4, 27.9, 27.0, 24.3, 23.9, 22.8, 22.5, 22.1, 21.6, 21.4, 18.9, 18.8, 18.2, 16.2, 14.2
5-5	δ_H 5.23 (s, 1H, 12-H), 4.46 (t, J = 7.0 Hz, 1H, 3-H), 4.02-4.08 (m, 2H), 2.70 (s, 1H), 2.56 (dd, J = 12.2, 4.8 Hz, 1H, H-18), 1.12-2.25 (m, 20H), 1.36-1.65 (m, 8H), 1.04 (s, 3H), 0.98 (s, 3H), 0.97 (s, 3H), 0.89 (s, H), 0.87 (s, 3H), 0.85 (s, 3H), 0.80 (s, 3H), 0.78 (s, 3H)	δ_C 177.6, 154.0, 138.5, 123.5, 84.2, 74.5, 74.0, 66.0, 56.2, 54.2, 50.1, 48.3, 45.4, 42.1, 41.8, 40.5, 39.7, 39.2, 38.2, 36.2, 35.1, 31.7, 29.3, 29.2, 28.4, 28.0, 27.5, 24.1, 23.6, 23.4, 22.7, 22.3, 21.5, 21.4, 19.3, 19.2, 18.6, 17.0, 12.7
Ⅰ₁	δ_H 7.79 (s, 1H, triazole-H), 7.05-7.53 (m, 5H), 5.31 (s, 1H, 12-H), 5.20 (d, J = 2.0 Hz, 2H), 4.49 (t, J = 7.2 Hz, 1 H, 3-H), 2.93 (dd, J = 11.0, 4.6 Hz, 1H, H-18), 2.06 (s, 3H), 1.07-2.01 (m, 20H), 0.94-1.05 (m, 2H), 1.02 (s, 3H), 0.91 (s, 3H), 0.90 (s, 3H), 0.89 (s, 3H), 0.86 (s, 3H), 0.85 (s, 3H), 0.82 (s, 3H)	δ_C 179.5, 174.2, 144.7, 134.4, 131.2, 128.4, 128.4, 127.0, 127.0, 122.9, 80.4, 63.1, 55.1, 47.3, 46.7, 46.4, 43.1, 42.0, 39.3, 38.0, 37.7, 36.1, 35.8, 34.1, 32.8, 32.7, 32.2, 30.6, 29.8, 28.0, 27.9, 25.6, 25.6, 23.7, 23.5, 23.5, 21.1, 18.0, 16.9, 16.6, 15.3
Ⅰ₂	δ_H 7.81 (s, 1H, triazole-H), 7.02-7.50 (m, 5H), 5.32 (s, 1H, 12-H), 5.19 (d, J = 3.2 Hz, 2H), 4.50 (t, J = 7.9 Hz, 1H, 3-H), 2.89 (dd, J = 9.2, 4.0 Hz, 1H, H-18), 2.31-2.27 (m, 2H), 1.04-2.05 (m, 20H), 1.22 (s, 3H), 0.94-1.07 (m, 2H), 1.01 (s, 3H), 0.91 (s, 3H), 0.90 (s, 3H), 0.87 (s, 3H), 0.87 (s, 3H), 0.85 (s, 3H), 0.80 (s, 3H)	δ_C 178.5, 171.0, 144.8, 132.3, 132.2, 126.1 126.1, 126.0, 126.0, 122.8, 115.8, 115.6, 80.8, 63.9, 55.1, 53.2, 47.4, 46.7, 46.5, 42.1, 42.0, 39.3, 38.8, 38.1, 37.7, 36.7, 34.1, 33.0, 32.6, 32.2, 28.0, 27.3, 25.7, 23.8, 23.6, 23.5, 23.4, 21.3, 18.1, 16.8, 16.6, 15.2
Ⅰ₃	δ_H 7.84 (s, 1H, triazole-H), 7.05-7.58 (m, 5H), 5.32 (s, 1H, 12-H), 5.23 (d, J = 3.0 Hz, 2H), 4.52 (t, J = 7.2 Hz, 1H, 3-H), 2.85 (dd, J = 8.0, 4.0 Hz, 1H, H-18), 2.35-2.29 (m, 2H), 1.06-2.01 (m, 20H), 1.61-1.68 (m, 2H), 1.60 (m, 2H), 1.23 (s, 3H), 1.15 (s, 3H), 0.95-1.07 (m, 2H), 0.94 (s, 3H), 0.89 (s, 3H), 0.87 (s, 3H), 0.85 (s, 3H), 0.84 (s, 3H), 0.78 (s, 3H)	δ_C 178.4, 170.9, 144.7, 132.7, 132.7, 132.7 132.2, 132.1, 127.7, 125.7, 125.7, 125.4, 125.4, 80.7, 55.0, 47.3, 46.6, 46.3, 42.0, 41.9, 39.2, 38.7, 38.0, 37.7, 37.5, 36.6, 32.8, 32.5, 32.1, 30.6, 27.9, 27.1, 26.8, 25.6, 23.7, 23.4, 23.4, 23.2, 21.2, 18.0, 16.7, 16.5, 15.1
Ⅰ₄	δ_H 7.82 (s, 1H, triazole-H), 7.01-7.50 (m, 5H), 5.32 (s, 1H, 12-H), 5.21 (d, J = 3.4 Hz, 2H), 4.51 (t, J = 7.5 Hz, 1 H, 3-H), 2.83 (dd, J = 8.0, 4.0 Hz, 1H, H-18), 2.33-2.25 (m, 2H), 1.01-2.05 (m, 20H), 1.35-1.80 (m, 4H), 1.25 (s, 3H), 1.16 (s, 3H), 0.92-1.04 (m, 2 H), 1.02 (s, 3H), 0.92 (s, 3H), 0.89 (s, 3H), 0.87 (s, 3H), 0.86 (s, 3H), 0.79 (s, 3H)	δ_C 178.5, 174.3, 144.7, 138.7, 134.9, 132.7, 131.2, 128.0, 128.0, 127.2, 127.2, 122.9, 80.5, 64.6, 55.1, 47.4, 46.7, 46.4, 42.1, 42.1, 42.0, 39.3, 38.6, 38.1, 37.8, 36.8, 34.1, 33.0, 32.6, 32.2, 32.2, 30.7, 28.1, 28.0, 27.3, 23.8, 23.8, 23.5, 23.4, 21.2, 18.1, 16.8, 16.7, 15.3
Ⅰ₅	δ_H 7.87 (s, 1H, triazole-H), 7.03-7.55 (m, 5H), 5.29 (s, 1H, 12-H), 5.19 (d, J = 3.2 Hz, 2H), 4.49 (t, J = 7.2 Hz, 1 H, 3-H), 2.82 (dd, J = 8.4, 4.3 Hz, 1H, H-18), 2.34-2.26 (m, 2H), 1.04-2.08 (m, 20H), 1.26-1.78 (m, 6H), 1.21 (s, 3H), 1.18 (s, 3H), 0.90-1.06 (m, 2 H), 0.96 (s, 3H), 0.93 (s, 3H), 0.89 (s, 3H), 0.86 (s, 3H), 0.85(s, 3H), 0.76 (s, 3H)	δ_C 179.7, 174.4, 144.9, 134.7, 131.4, 128.7, 128.7, 127.2, 127.2, 123.1, 80.6, 63.3, 55.3, 47.6, 46.9, 46.6, 42.2, 42.2, 39.5, 38.3, 37.9, 36.3, 36.0, 34.3, 33.1, 33.0, 32.4, 32.4, 30.8, 30.1, 28.2, 28.1, 27.5, 27.5, 25.8, 25.8, 23.9, 23.7, 23.6, 18.3, 17.1, 16.8, 16.8, 15.5, 9.5
Ⅱ₁	δ_H 7.88 (s, 1H, triazole-H), 7.10-7.55 (m, 5H), 5.43 (s, 1H, 12-H), 4.41 (t, J = 8.0 Hz, 1H, 3-H), 4.25-4.19 (m, 2H), 3.63 (t, J = 7.2 Hz, 2H), 2.58 (dd, J = 12.0, 4.0 Hz, 1H, H-18), 1.01-2.02 (m, 20H), 1.20 (s, 3H), 1.15 (s, 3H), 0.92-1.08 (m, 2H), 0.98 (s, 3H), 0.91 (s, 3H), 0.89 (s, 3H), 0.84 (s, 3H), 0.80(s, 3H), 0.71 (s, 3H)	δ_C 179.5, 174.2, 144.7, 133.5, 131.2, 128.4, 128.4, 127.0, 127.0, 125.9, 125.9, 80.4, 64.4, 55.1, 47.4, 46.7, 46.4, 42.1, 42.0, 42.0, 39.3, 38.0, 37.7, 36.8, 36.1, 35.8, 32.9, 32.8, 32.2, 30.6, 29.9, 28.0, 27.9, 25.6, 23.7, 23.5, 23.4, 21.3, 21.1, 18.6, 16.9, 15.3
Ⅱ₂	δ_H 7.83 (s, 1H, triazole-H), 7.03-7.53 (m, 5H), 5.21 (s, 1H, 12-H), 4.29 (t, J = 8.2 Hz, 1H, 3-H), 4.24-4.17 (m, 2H), 3.60 (t, J = 7.0 Hz, 2H), 2.55 (dd, J = 12.4, 3.5 Hz, 1H, H-18), 1.03-2.06 (m, 20H), 1.72 (m, 2 H), 1.19 (s, 3H), 1.14 (s, 3H), 0.90-1.10 (m, 2H), 0.98 (s, 3H), 0.93 (s, 3H), 0.91 (s, 3H), 0.89 (s, 3H), 0.87 (s, 3H), 0.85 (s, 3H)	δ_C 178.6, 171.2, 144.9, 132.4, 132.4 132.3, 132.3, 126.2, 126.2, 126.1, 125.9, 121.9, 121.9, 121.8, 80.9, 64.0, 55.2, 47.5, 46.9, 46.6, 42.2, 42.1, 39.4, 39.0, 37.8, 36.9, 34.2, 33.1, 32.7, 32.3, 30.8, 28.1, 27.3, 26.7, 25.8, 23.9, 23.6, 23.6, 23.5, 18.2, 16.9, 15.3, 9.33
Ⅱ₃	δ_H 7.86 (s, 1H, triazole-H), 7.09-7.45 (m, 5H), 5.24 (s, 1H, 12-H), 4.23 (t, J = 8.2 Hz, 1 H, 3-H), 4.20-4.10 (m, 2H), 3.58 (t, J = 7.2 Hz, 2H), 2.52 (dd, J = 12.0, 4.5 Hz, 1H, H-18), 1.05-2.10 (m, 20H), 1.32-1.59 (m, 4H), 1.15 (s, 3H), 1.10 (s, 3H), 0.91-1.07 (m, 2H), 0.95 (s, 3H), 0.93 (s, 3H), 0.90 (s, 3H), 0.89 (s, 3H), 0.87 (s, 3H), 0.83 (s, 3H)	δ_C 179.4, 171.0, 144.7, 136.4, 134.2, 131.8, 130.7, 130.0, 130.0, 127.4, 127.4, 122.9, 121.4, 119.7, 80.8, 65.0, 55.2, 47.5, 46.7, 46.4, 42.1, 39.4, 39.4, 38.1, 37.7, 36.9, 36.5, 34.1, 30.7, 29.9, 29.9, 28.0, 27.3, 25.7, 25.7, 24.8, 23.7, 23.6, 23.6, 21.3, 18.2, 17.0, 16.7, 15.4
Ⅱ₄	δ_H 7.84 (s, 1H, triazole-H), 7.13-7.50 (m, 5H), 5.31 (s, 1H, 12-H), 4.26 (t, J = 8.0 Hz, 1H, 3-H), 4.23-4.12 (m, 2H), 3.55 (t, J = 7.2 Hz, 2H), 2.50 (dd, J = 12.0, 4.0 Hz, 1H, H-18), 1.02-2.14 (m, 20H), 1.35-1.69 (m, 6H), 1.12 (s, 3H), 0.95-1.05 (m, 2H), 0.99 (s, 3H), 0.98 (s, 3H), 0.94 (s, 3H), 0.91 (s, 3H), 0.89 (s, 3H), 0.79 (s, 3H), 0.77 (s, 3H)	δ_C 179.6, 174.3, 144.8, 134.6, 131.3, 128.5, 128.5, 127.1, 127.1, 123.0, 80.5, 65.2, 55.2, 47.5, 46.8, 46.5, 42.1, 42.1, 39.4, 39.4, 38.1, 37.8, 36.9, 36.2, 35.9, 33.0, 32.9, 30.7, 30.0, 30.0, 28.1, 27.4, 26.8, 25.7, 25.7, 23.8, 23.6, 23.5, 18.2, 18.2, 17.0, 16.7, 15.4, 15.4, 9.4
Ⅱ₅	δ_H 7.91 (s, 1H, triazole-H), 7.10-7.59 (m, 5H), 5.43 (s, 1H, 12-H), 4.32 (t, J = 8.2 Hz, 1H, 3-H), 4.30-4.16 (m, 2H), 3.58 (t, J = 7.2 Hz, 2H), 2.55 (dd, J = 12.4, 4.2 Hz, 1H, H-18), 1.01-2.07 (m, 20H), 1.28-1.65 (m, 8H), 1.10 (s, 3H), 0.94-1.06 (m, 2H), 0.99 (s, 3H), 0.98 (s, 3H), 0.97 (s, 3H), 0.91 (s, 3H), 0.90 (s, 3H), 0.79 (s, 3H), 0.75 (s, 3H)	δ_C 179.4, 171.1, 144.7, 136.4, 134.2, 131.8, 130.7, 130.7, 130.0, 130.0, 127.4, 127.4, 122.9, 80.9, 66.2, 55.2, 47.5, 46.7, 46.4, 42.1, 42.1, 42.0, 39.4, 38.1, 37.7, 36.9, 36.5, 34.1, 33.0, 32.9, 32.9, 30.7, 30.0, 28.0, 27.3, 25.7, 24.8, 23.7, 21.3, 21.3, 18.2, 17.0, 16.7, 15.4, 15.4, 9.4

Compd.

¹H NMR (600 MHz, CDCl₃)

¹³C NMR (150 MHz, CDCl₃)

3-1

3-2

3-3

3-4

3-5

5-1

5-2

5-3

5-4

5-5

Ⅰ₁

Ⅰ₂

Ⅰ₃

Ⅰ₄

Ⅰ₅

Ⅱ₁

Ⅱ₂

Ⅱ₃

Ⅱ₄

Ⅱ₅

Compd.	IC₅₀/μmol·L^-1		Compd.	IC₅₀/μmol·L^-1
Ⅰ₁	40.22 ± 0.11	37.02 ± 0.07		Ⅱ₁	39.27 ± 0.19	30.18 ± 0.22
Ⅰ₂	31.48 ± 0.10	20.13 ± 0.13	Ⅱ₂	33.32 ± 0.15	21.45 ± 0.08
Ⅰ₃	30.15 ± 0.15	19.21 ± 0.13	Ⅱ₃	23.13 ± 0.20	20.28 ± 0.15
Ⅰ₄	18.22 ± 0.09	12.72 ± 0.15	Ⅱ₄	14.28 ± 0.14	8.46 ± 0.10
Ⅰ₅	38.65 ± 0.16	25.12 ± 0.08	Ⅱ₅	20.68 ± 0.19	19.54 ± 0.17
UA	> 50	> 50	Nilotinib	15.40 ± 0.10	10.15 ± 0.08

Compd.

IC₅₀/μmol·L^-1

Compd.

IC₅₀/μmol·L^-1

MCF-7

SGC-7901

MCF-7

SGC-7901

Ⅰ₁

40.22 ± 0.11

37.02 ± 0.07

Ⅱ₁

39.27 ± 0.19

30.18 ± 0.22

Ⅰ₂

31.48 ± 0.10

20.13 ± 0.13

Ⅱ₂

33.32 ± 0.15

21.45 ± 0.08

Ⅰ₃

30.15 ± 0.15

19.21 ± 0.13

Ⅱ₃

23.13 ± 0.20

20.28 ± 0.15

Ⅰ₄

18.22 ± 0.09

12.72 ± 0.15

Ⅱ₄

14.28 ± 0.14

8.46 ± 0.10

Ⅰ₅

38.65 ± 0.16

25.12 ± 0.08

Ⅱ₅

20.68 ± 0.19

19.54 ± 0.17

> 50

Nilotinib

15.40 ± 0.10

10.15 ± 0.08

Compd.	Docking score		Compd.	Docking score
Ⅰ₁	-154.697		Ⅱ₁	-155.338
Ⅰ₂	-162.983	Ⅱ₂	-160.921
Ⅰ₃	-162.793	Ⅱ₃	-161.544
Ⅰ₄	-167.873	Ⅱ₄	-172.941
Ⅰ₅	-161.868	Ⅱ₅	-165.844
Nilotinib	-170.459	UA	-120.341

Compd.

Docking score

Compd.

Docking score

Ⅰ₁

-154.697

Ⅱ₁

-155.338

Ⅰ₂

-162.983

Ⅱ₂

-160.921

Ⅰ₃

-162.793

Ⅱ₃

-161.544

Ⅰ₄

-167.873

Ⅱ₄

-172.941

Ⅰ₅

-161.868

Ⅱ₅

-165.844

Nilotinib

-170.459

-120.341