首页 期刊 论文 学科刊群 资讯 加盟 关于
EN
image
Article(id=1320389042328420607, tenantId=1146029695717560320, journalId=1319373289681944729, issueId=1320388933154878311, articleNumber=null, orderNo=null, doi=10.11876/mimt202504012, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=null, receivedDateStr=null, revisedDate=null, revisedDateStr=null, acceptedDate=null, acceptedDateStr=null, onlineDate=1792734879332, onlineDateStr=2026-10-23, pubDate=1754755200000, pubDateStr=2025-08-10, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1792734879332, onlineIssueDateStr=2026-10-23, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1792734879332, creator=13701087609, updateTime=1792734879332, updator=13701087609, issue=Issue{id=1320388933154878311, tenantId=1146029695717560320, journalId=1319373289681944729, year='2025', volume='31', issue='4', pageStart='2', pageEnd='132', issueExtLink='null', onlineDate='null', pubDate='1754755200000', pubDateStr='2025-08-10', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1792734853304, creator='13701087609', updateTime=1792746408152, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1320437397758791917, tenantId=1146029695717560320, journalId=1319373289681944729, issueId=1320388933154878311, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1320437397758791918, tenantId=1146029695717560320, journalId=1319373289681944729, issueId=1320388933154878311, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=111, endPage=116, ext={EN=ArticleExt(id=1320389042596856064, articleId=1320389042328420607, tenantId=1146029695717560320, journalId=1319373289681944729, language=EN, title=Applications and prospects of two-photon intravital imaging in the field of life sciences, columnId=null, journalTitle=Modern Instruments & Medical Treatment, columnName=null, runingTitle=null, highlight=null, articleAbstract=

Intravital two-photon imaging technology has the advantage of deep imaging, enabling the direct monitoring of the dynamic processes within living organisms. It has become an important research tool in the field of life sciences. We briefly explain the basic principles of two-photon microscopy and its applications in life science research in recent years. We mainly focuse on analyzing the development trends of intravital two-photon imaging technology and discuss the current challenges and future optimization directions. We look forward to the continuous optimization of intravital two-photon imaging technology, which can promote its wider application.

, authors=Wenjuan Bai, Jin Wang, Wenying Zhao, Jing Wang*, authorsList=Wenjuan Bai, Jin Wang, Wenying Zhao, Jing Wang, authorCompany=null, correspAuthors=Jing Wang, authorNote=null, correspAuthorsNote=null, copyrightStatement=All copyrights in 2025 belong to the sponsor of the journal.Not content published by the journal may be reproduced or abridged without authorization., copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1320389043045646593, articleId=1320389042328420607, tenantId=1146029695717560320, journalId=1319373289681944729, language=CN, title=双光子活体成像在生命科学领域的应用与展望, columnId=1320389006882357264, journalTitle=现代仪器与医疗, columnName=生物应用, runingTitle=null, highlight=null, articleAbstract=

双光子活体成像技术具有深层成像的优势，可以直观地监测活体的体内动态过程，已成为生命科学领域研究的重要手段。本文简要介绍了双光子显微镜的技术原理，综述了双光子显微镜在生命科学研究中的应用领域，重点分析了双光子显微活体成像技术的发展趋势，探讨其当前面临的挑战及未来的优化方向，旨在为进一步优化双光子活体成像技术，推动双光子活体成像技术更加广泛的应用提供参考。

, authors=柏文娟, 王靳, 赵文颖, 王静*, authorsList=柏文娟, 王靳, 赵文颖, 王静, authorCompany=null, correspAuthors=王静, authorNote=

柏文娟，女，博士研究生，实验师，研究方向：主要从事显微成像平台、大型显微镜的管理工作

, correspAuthorsNote=
王静，女，博士研究生，研究员，研究方向：运用活体成像研究天然免疫细胞的动态及功能
, copyrightStatement=2025年版权归本刊主办单位所有未经本刊书面许可, 不得转载、摘编本刊所有文章内容。, copyrightOwner=中国科学器材公司, extLink=null, articleAbsUrl=null, sourceXml=hCgr3JaLbVjK5iIBAqsy2w==, magXml=m3l5urEbD2u88BZfKelx3Q==, pdfUrl=null, pdf=Ry0sEAcBGfW/6lVsjp61aw==, pdfFileSize=1606657, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=noBD0fhHwyebLYLn0CgEQg==, mapNumber=null, fund=null)}, authors=[Author(id=1320389043557351686, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, orderNo=0, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1320389045268627720, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, authorId=1320389043557351686, language=EN, stringName=Wenjuan Bai, firstName=Wenjuan, middleName=null, lastName=Bai, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=null, address=Shanghai Institute of Immunology, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1320389045436399881, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, authorId=1320389043557351686, language=CN, stringName=柏文娟, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=null, address=上海交通大学医学院上海市免疫学研究所，上海　200025, bio={"content":"

柏文娟，女，博士研究生，实验师，研究方向：主要从事显微成像平台、大型显微镜的管理工作

"}, bioImg=null, bioContent=

柏文娟，女，博士研究生，实验师，研究方向：主要从事显微成像平台、大型显微镜的管理工作

, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1320389043389579522, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, xref=null, ext=[AuthorCompanyExt(id=1320389043423133955, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, companyId=1320389043389579522, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=Shanghai Institute of Immunology, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China), AuthorCompanyExt(id=1320389043444105476, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, companyId=1320389043389579522, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=上海交通大学医学院上海市免疫学研究所，上海　200025)])]), Author(id=1320389045553840395, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, orderNo=1, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1320389045725806861, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, authorId=1320389045553840395, language=EN, stringName=Jin Wang, firstName=Jin, middleName=null, lastName=Wang, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=null, address=Shanghai Institute of Immunology, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1320389045851635982, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, authorId=1320389045553840395, language=CN, stringName=王靳, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=null, address=上海交通大学医学院上海市免疫学研究所，上海　200025, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1320389043389579522, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, xref=null, ext=[AuthorCompanyExt(id=1320389043423133955, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, companyId=1320389043389579522, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=Shanghai Institute of Immunology, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China), AuthorCompanyExt(id=1320389043444105476, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, companyId=1320389043389579522, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=上海交通大学医学院上海市免疫学研究所，上海　200025)])]), Author(id=1320389045960687888, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, orderNo=2, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1320389046115877138, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, authorId=1320389045960687888, language=EN, stringName=Wenying Zhao, firstName=Wenying, middleName=null, lastName=Zhao, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=null, address=Shanghai Institute of Immunology, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1320389046287843603, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, authorId=1320389045960687888, language=CN, stringName=赵文颖, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=null, address=上海交通大学医学院上海市免疫学研究所，上海　200025, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1320389043389579522, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, xref=null, ext=[AuthorCompanyExt(id=1320389043423133955, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, companyId=1320389043389579522, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=Shanghai Institute of Immunology, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China), AuthorCompanyExt(id=1320389043444105476, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, companyId=1320389043389579522, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=上海交通大学医学院上海市免疫学研究所，上海　200025)])]), Author(id=1320389046430449941, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, orderNo=3, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=1, authorType=1, ext={EN=AuthorExt(id=1320389046606610711, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, authorId=1320389046430449941, language=EN, stringName=Jing Wang, firstName=Jing, middleName=null, lastName=Wang, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=*, address=Shanghai Institute of Immunology, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1320389046753411352, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, authorId=1320389046430449941, language=CN, stringName=王静, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=*, address=上海交通大学医学院上海市免疫学研究所，上海　200025, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1320389043389579522, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, xref=null, ext=[AuthorCompanyExt(id=1320389043423133955, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, companyId=1320389043389579522, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=Shanghai Institute of Immunology, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China), AuthorCompanyExt(id=1320389043444105476, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, companyId=1320389043389579522, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=上海交通大学医学院上海市免疫学研究所，上海　200025)])])], keywords=[Keyword(id=1320389047005069593, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, language=EN, orderNo=1, keyword=Intravital imaging), Keyword(id=1320389047172841754, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, language=EN, orderNo=2, keyword=Two-photon microscope), Keyword(id=1320389047323836699, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, language=EN, orderNo=3, keyword=Life Sciences), Keyword(id=1320389047504191772, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, language=CN, orderNo=1, keyword=活体成像), Keyword(id=1320389047709712669, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, language=CN, orderNo=2, keyword=双光子显微镜), Keyword(id=1320389047831347486, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, language=CN, orderNo=3, keyword=生命科学)], refs=[Reference(id=1320389049840419104, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[1], rfOrder=0, authorNames=null, journalName=null, refType=null, unstructuredReference=Denk W, Strickler J H, Webb W W. Two-photon laser scanning fluorescence microscopy[J]. Science, 1990, 248(4951): 73-76., articleTitle=null, refAbstract=null), Reference(id=1320389050226295073, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[2], rfOrder=1, authorNames=null, journalName=null, refType=null, unstructuredReference=Kuan A T, Bondanelli G, Driscoll L N, et al. Synaptic wiring motifs in posterior parietal cortex support decision-making[J]. Nature, 2024, 627: 367-373., articleTitle=null, refAbstract=null), Reference(id=1320389050305986850, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[3], rfOrder=2, authorNames=null, journalName=null, refType=null, unstructuredReference=Shainer I, Kappel J M, Laurell E, et al. Transcriptomic neuron types vary topographically in function and morphology[J]. Nature, 2025, 638: 1023-1033., articleTitle=null, refAbstract=null), Reference(id=1320389050394067235, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[4], rfOrder=3, authorNames=null, journalName=null, refType=null, unstructuredReference=Drieu C, Zhu Z, Wang Z, et al. Rapid emergence of latent knowledge in the sensory cortex drives learning[J]. Nature, 2025, 641: 960-970., articleTitle=null, refAbstract=null), Reference(id=1320389050473759012, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[5], rfOrder=4, authorNames=null, journalName=null, refType=null, unstructuredReference=Sun W, Winnubst J, Natrajan M, et al. Learning produces an orthogonalized state machine in the hippocampus[J]. Nature, 2025, 640: 165-175., articleTitle=null, refAbstract=null), Reference(id=1320389050645725477, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[6], rfOrder=5, authorNames=null, journalName=null, refType=null, unstructuredReference=Xiong H, Tang F, Guo Y, et al. Neural circuit changes in neurological disorders: evidence from in vivo two-photon imaging[J]. Ageing Res Rev, 2023, 87: 101933., articleTitle=null, refAbstract=null), Reference(id=1320389050729611558, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[7], rfOrder=6, authorNames=null, journalName=null, refType=null, unstructuredReference=Haage T R, Charakopoulos E, Bhuria V, et al. Neutrophil-specific expression of JAK2-V617F or CALRmut induces distinct inflammatory profiles in myeloproliferative neoplasia[J]. J Hematol Oncol, 2024, 17: 43., articleTitle=null, refAbstract=null), Reference(id=1320389050800914727, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[8], rfOrder=7, authorNames=null, journalName=null, refType=null, unstructuredReference=Cheng L, Liu W, Zhong C, et al. Remodeling the homeostasis of pro- and anti-angiogenic factors by Shenmai injection to normalize tumor vasculature for enhanced cancer chemotherapy[J]. J Ethnopharmacol, 2021, 270: 113770., articleTitle=null, refAbstract=null), Reference(id=1320389050876412200, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[9], rfOrder=8, authorNames=null, journalName=null, refType=null, unstructuredReference=Schoofs H, Daubel N, Schnabellehner S, et al. Dynamic cytoskeletal regulation of cell shape supports resilience of lymphatic endothelium[J]. Nature, 2025, 641: 465-475., articleTitle=null, refAbstract=null), Reference(id=1320389051069350185, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[10], rfOrder=9, authorNames=null, journalName=null, refType=null, unstructuredReference=Pae J, Schwan N, Ottino-Loffler B, et al. Transient silencing of hypermutation preserves B cell affinity during clonal bursting[J]. Nature, 2025, 641: 486-494., articleTitle=null, refAbstract=null), Reference(id=1320389051132264746, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[11], rfOrder=10, authorNames=null, journalName=null, refType=null, unstructuredReference=Wu T, Liao J, Xiang F, et al. Short-wavelength excitation two-photon intravital microscopy of endogenous fluorophores[J]. Biomed Opt Express, 2023, 14(7): 3380-3396., articleTitle=null, refAbstract=null), Reference(id=1320389051245510955, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[12], rfOrder=11, authorNames=null, journalName=null, refType=null, unstructuredReference=Wang Y, Heymann F, Peiseler M. Intravital imaging: dynamic insights into liver immunity in health and disease[J]. Gut, 2024, 73(8): 1364-1375., articleTitle=null, refAbstract=null), Reference(id=1320389051480391980, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[13], rfOrder=12, authorNames=null, journalName=null, refType=null, unstructuredReference=Liu D, Winer B Y, Chou M Y, et al. Dynamic encounters with red blood cells trigger splenic marginal zone B cell retention and function[J]. Nat Immunol, 2024, 25(1): 142-154., articleTitle=null, refAbstract=null), Reference(id=1320389051539112237, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[14], rfOrder=13, authorNames=null, journalName=null, refType=null, unstructuredReference=Gaertner F, Ishikawa-Ankerhold H, Stutte S, et al. Plasmacytoid dendritic cells control homeostasis of megakaryopoiesis[J]. Nature, 2024, 631: 645-653., articleTitle=null, refAbstract=null), Reference(id=1320389051622998318, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[15], rfOrder=14, authorNames=null, journalName=null, refType=null, unstructuredReference=Asquith N L, Carminita E, Camacho V, et al. The bone marrow is the primary site of thrombopoiesis[J]. Blood, 2024, 143(3): 272-278., articleTitle=null, refAbstract=null), Reference(id=1320389051685912879, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[16], rfOrder=15, authorNames=null, journalName=null, refType=null, unstructuredReference=Santocki M, Such A, Drab D, et al. NETs persisting in vasculature undergo self-renewal with consequences for subsequent infection: a mouse model study[J]. Blood, 2025, 145(18): 2070-2085., articleTitle=null, refAbstract=null), Reference(id=1320389051748827440, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[17], rfOrder=16, authorNames=null, journalName=null, refType=null, unstructuredReference=van de Wall S, Anthony S M, Hancox L S, et al. Dynamic landscapes and protective immunity coordinated by influenza-specific lung-resident memory CD8+ T cells revealed by intravital imaging[J]. Immunity, 2024, 57(8): 1878-1892., articleTitle=null, refAbstract=null), Reference(id=1320389051954348337, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[18], rfOrder=17, authorNames=null, journalName=null, refType=null, unstructuredReference=Uderhardt S, Neag G, Germain R N. Dynamic multiplex tissue imaging in inflammation research[J]. Annu Rev Pathol, 2024, 19: 43-67., articleTitle=null, refAbstract=null), Reference(id=1320389052025651506, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[19], rfOrder=18, authorNames=null, journalName=null, refType=null, unstructuredReference=Zhu X, Tang Y, Chen J, et al. Monitoring wound healing of elastic cartilage using multiphoton microscopy[J]. Osteoarthritis and Cartilage, 2013, 21(11): 1799-1806., articleTitle=null, refAbstract=null), Reference(id=1320389052084371763, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[20], rfOrder=19, authorNames=null, journalName=null, refType=null, unstructuredReference=Vicanolo T, Özcan A, Li J L, et al. Matrix-producing neutrophils populate and shield the skin[J]. Nature, 2025, 641: 740-748., articleTitle=null, refAbstract=null), Reference(id=1320389052147286324, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[21], rfOrder=20, authorNames=null, journalName=null, refType=null, unstructuredReference=Pappinen S, Pryazhnikov E, Khiroug L, et al. Organotypic cell cultures and two-photon imaging: tools for in vitro and in vivo assessment of percutaneous drug delivery and skin toxicity[J]. Journal of Controlled Release, 2012, 161(2): 656-667., articleTitle=null, refAbstract=null), Reference(id=1320389052201812277, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[22], rfOrder=21, authorNames=null, journalName=null, refType=null, unstructuredReference=Di Martino J S, Nobre A R, Mondal C, et al. A tumor-derived type III collagen-rich ECM niche regulates tumor cell dormancy[J]. Nat Cancer, 2022, 3: 90-107., articleTitle=null, refAbstract=null), Reference(id=1320389052365390134, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[23], rfOrder=22, authorNames=null, journalName=null, refType=null, unstructuredReference=Dalla E, Papanicolaou M, Park M D, et al. Lung-resident alveolar macrophages regulate the timing of breast cancer metastasis[J]. Cell, 2024, 187(23): 6631-6648., articleTitle=null, refAbstract=null), Reference(id=1320389052432498999, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[24], rfOrder=23, authorNames=null, journalName=null, refType=null, unstructuredReference=Isshiki Y, Chen X, Teater M, et al. EZH2 inhibition enhances T cell immunotherapies by inducing lymphoma immunogenicity and improving T cell function[J]. Cancer Cell, 2025, 43(1): 49-68., articleTitle=null, refAbstract=null), Reference(id=1320389052507996472, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[25], rfOrder=24, authorNames=null, journalName=null, refType=null, unstructuredReference=Kim H R, Park J S, Park J H, et al. Cell-permeable transgelin-2 as a potent therapeutic for dendritic cell-based cancer immunotherapy[J]. J Hematol Oncol, 2021, 14(1): 43., articleTitle=null, refAbstract=null), Reference(id=1320389052566716729, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[26], rfOrder=25, authorNames=null, journalName=null, refType=null, unstructuredReference=Wu Y X, Zhang D, Hu X, et al. Multicolor two-photon nanosystem for multiplexed intracellular imaging and targeted cancer therapy[J]. Angew Chem Int Ed Engl, 2021, 60(22): 12569-12576., articleTitle=null, refAbstract=null), Reference(id=1320389054236049722, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[27], rfOrder=26, authorNames=null, journalName=null, refType=null, unstructuredReference=Fang F, Yuan Y, Wan Y, et al. Near-infrared thermally activated delayed fluorescence nanoparticle: a metal-free photosensitizer for two-photon-activated photodynamic therapy at the cell and small animal levels[J]. Small, 2022, 18(6): e2106215., articleTitle=null, refAbstract=null), Reference(id=1320389054307352891, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[28], rfOrder=27, authorNames=null, journalName=null, refType=null, unstructuredReference=Wei W, Hao M, Zhou K, et al. In situ multimodal transparent electrophysiological hydrogel for in vivo miniature two-photon neuroimaging and electrocorticogram analysis[J]. Acta Biomater, 2022, 152: 86-99., articleTitle=null, refAbstract=null), Reference(id=1320389054370267452, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[29], rfOrder=28, authorNames=null, journalName=null, refType=null, unstructuredReference=Liu Z, Lu X, Villette V, et al. Sustained deep-tissue voltage recording using a fast indicator evolved for two-photon microscopy[J]. Cell, 2022, 185(18): 3408-3425., articleTitle=null, refAbstract=null), Reference(id=1320389054420599101, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[30], rfOrder=29, authorNames=null, journalName=null, refType=null, unstructuredReference=Waters J. A large field of view 2- and 3-photon microscope[J]. Light Sci Appl, 2025, 14(1): 106., articleTitle=null, refAbstract=null), Reference(id=1320389054496096574, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[31], rfOrder=30, authorNames=null, journalName=null, refType=null, unstructuredReference=Choe K, Hontani Y, Wang T, et al. Intravital three-photon microscopy allows visualization over the entire depth of mouse lymph nodes[J]. Nat Immunol, 2022, 23(2): 330-340., articleTitle=null, refAbstract=null), Reference(id=1320389054571594047, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[32], rfOrder=31, authorNames=null, journalName=null, refType=null, unstructuredReference=Zhao Z, Zhou Y, Liu B, et al. Two-photon synthetic aperture microscopy for minimally invasive fast 3D imaging of native subcellular behaviors in deep tissue[J]. Cell, 2023, 186(11): 2475-2491., articleTitle=null, refAbstract=null), Reference(id=1320389054651285824, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[33], rfOrder=32, authorNames=null, journalName=null, refType=null, unstructuredReference=Olesiak-Banska J, Waszkielewicz M, Obstarczyk P, et al. Twophoton absorption and photoluminescence of colloidal gold nanoparticles and nanoclusters[J]. Chem Soc Rev, 2019, 48(15): 4087-4117., articleTitle=null, refAbstract=null), Reference(id=1320389054747754817, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[34], rfOrder=33, authorNames=null, journalName=null, refType=null, unstructuredReference=Lu J, Li Z, Lu M, et al. Assessing early atherosclerosis by detecting and imaging of hypochlorous acid and phosphorylation using fluorescence nanoprobe[J]. Adv Mater, 2023, 35(52): e2307008., articleTitle=null, refAbstract=null), Reference(id=1320389054844223810, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[35], rfOrder=34, authorNames=null, journalName=null, refType=null, unstructuredReference=Hong S T, Kim T H, Choi J W, et al. Two-photon probes for pH: detection of human colon cancer using two-photon microscopy[J]. Anal Chem, 2017, 89(18): 9830-9835., articleTitle=null, refAbstract=null), Reference(id=1320389054923915587, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[36], rfOrder=35, authorNames=null, journalName=null, refType=null, unstructuredReference=Larson D R, Zipfel W R, Williams R M, et al. Water-soluble quantum dots for multiphoton fluorescence imaging in vivo[J]. Science, 2003, 300(5624): 1434-1436., articleTitle=null, refAbstract=null), Reference(id=1320389054986830148, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[37], rfOrder=36, authorNames=null, journalName=null, refType=null, unstructuredReference=Juvekar V, Park S J, Yoon J, et al. Recent progress in the two-photon fluorescent probes for metal ions[J]. Coordination Chemistry Reviews, 2021, 427: 213574., articleTitle=null, refAbstract=null), Reference(id=1320389055062327621, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[38], rfOrder=37, authorNames=null, journalName=null, refType=null, unstructuredReference=Mann K, Deny S, Ganguli S, et al. Coupling of activity, metabolism and behaviour across the Drosophila brain[J]. Nature, 2021, 593: 244-248., articleTitle=null, refAbstract=null), Reference(id=1320389055125242182, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[39], rfOrder=38, authorNames=null, journalName=null, refType=null, unstructuredReference=Kagan T, Gabay M, Meenakshisundaram A, et al. Genetically encoded biosensor for fluorescence lifetime imaging of PTEN dynamics in the intact brain[J]. Nat Methods, 2025, 22: 764-777., articleTitle=null, refAbstract=null), Reference(id=1320389055192351047, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[40], rfOrder=39, authorNames=null, journalName=null, refType=null, unstructuredReference=Zong W, Wu R, Li M, et al. Fast high-resolution miniature two-photon microscopy for brain imaging in freely behaving mice[J]. Nat Methods, 2017, 14: 713-719., articleTitle=null, refAbstract=null), Reference(id=1320389055263654216, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[41], rfOrder=40, authorNames=null, journalName=null, refType=null, unstructuredReference=Zong W, Wu R, Chen S, et al. Miniature two-photon microscopy for enlarged field-of-view, multi-plane and long-term brain imaging[J]. Nat Methods, 2021, 18: 46-49., articleTitle=null, refAbstract=null), Reference(id=1320389055347540297, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[42], rfOrder=41, authorNames=null, journalName=null, refType=null, unstructuredReference=Zong W, Obenhaus H A, Skytøen E R, et al. Large-scale two-photon calcium imaging in freely moving mice[J]. Cell, 2022, 185(7): 1240-1256., articleTitle=null, refAbstract=null), Reference(id=1320389055431426378, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[43], rfOrder=42, authorNames=null, journalName=null, refType=null, unstructuredReference=Gu X, Zhao Z, Chen X, et al. Imaging microglia surveillance during sleep-wake cycles in freely behaving mice[J]. eLife, 2023, 12: RP86749., articleTitle=null, refAbstract=null), Reference(id=1320389055515312459, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[44], rfOrder=43, authorNames=null, journalName=null, refType=null, unstructuredReference=Cheng N, Dong Q, Zhang Z, et al. Egocentric processing of items in spines, dendrites, and somas in the retrosplenial cortex[J]. Neuron, 2024, 112(4): 646-660., articleTitle=null, refAbstract=null), Reference(id=1320389055620170060, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[45], rfOrder=44, authorNames=null, journalName=null, refType=null, unstructuredReference=Zhao Z, Zeng F, Wang H, et al. Encoding of social novelty by sparse GABAergic neural ensembles in the prelimbic cortex[J]. Sci Adv, 2022, 8(35): eabo4884., articleTitle=null, refAbstract=null), Reference(id=1320389055691473229, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[46], rfOrder=45, authorNames=null, journalName=null, refType=null, unstructuredReference=Zhang Z, Jiang S, Shi K, et al. Visualizing seizure propagation in freely-moving mice via miniature two-photon microscopy[J]. Neurosci Bull, 2022, 38: 1593-1597., articleTitle=null, refAbstract=null), Reference(id=1320389055762776398, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[47], rfOrder=46, authorNames=null, journalName=null, refType=null, unstructuredReference=Ueki H, Wang I H, Zhao D, et al. Multicolor two-photon imaging of in vivo cellular pathophysiology upon influenza virus infection using the two-photon IMPRESS[J]. Nat Protoc, 2020, 15: 1041-1065., articleTitle=null, refAbstract=null), Reference(id=1320389055884411215, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[48], rfOrder=47, authorNames=null, journalName=null, refType=null, unstructuredReference=Song X, Guo Y, Chen C, et al. A silent two-photon imaging system for studying in vivo auditory neuronal functions[J]. Light Sci Appl, 2022, 11: 96., articleTitle=null, refAbstract=null), Reference(id=1320389055968297296, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[49], rfOrder=48, authorNames=null, journalName=null, refType=null, unstructuredReference=Papagiakoumou E, Ronzitti E, Emiliani V. Scanless two-photon excitation with temporal focusing[J]. Nat Methods, 2020, 17: 571-581., articleTitle=null, refAbstract=null), Reference(id=1320389056043794769, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[50], rfOrder=49, authorNames=null, journalName=null, refType=null, unstructuredReference=Platisa J, Ye X, Ahrens A M, et al. High-speed low-light in vivo two-photon voltage imaging of large neuronal populations[J]. Nat Methods, 2023, 20: 1095-1103., articleTitle=null, refAbstract=null), Reference(id=1320389056115097938, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[51], rfOrder=50, authorNames=null, journalName=null, refType=null, unstructuredReference=Beaulieu D R, Davison I G, Kılıç K, et al. Simultaneous multiplane imaging with reverberation two-photon microscopy[J]. Nat Methods, 2020, 17: 283-286., articleTitle=null, refAbstract=null), Reference(id=1320389056190595411, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[52], rfOrder=51, authorNames=null, journalName=null, refType=null, unstructuredReference=Lanore F, Cayco-Gajic N A, Gurnani H, et al. Cerebellar granule cell axons support high-dimensional representations[J]. Nat Neurosci, 2021, 24: 1142-1150., articleTitle=null, refAbstract=null), Reference(id=1320389056266092884, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[53], rfOrder=52, authorNames=null, journalName=null, refType=null, unstructuredReference=Griffiths V A, Valera A M, Lau J Y, et al. Real-time 3D movement correction for two-photon imaging in behaving animals[J]. Nat Methods, 2020, 17: 741-748, articleTitle=null, refAbstract=null), Reference(id=1320389056379339094, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[54], rfOrder=53, authorNames=null, journalName=null, refType=null, unstructuredReference=Mok A T, Wang T, Zhao S, et al. A large field-of-view, single-cell-resolution two- and three-photon microscope for deep and wide imaging[J]. eLight, 2024, 4: 20., articleTitle=null, refAbstract=null), Reference(id=1320389056538722647, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[55], rfOrder=54, authorNames=null, journalName=null, refType=null, unstructuredReference=Qin Z, He S, Yang C, et al. Adaptive optics two-photon microscopy enables near-diffraction-limited and functional retinal imaging in vivo[J]. Light Sci Appl, 2020, 9: 79., articleTitle=null, refAbstract=null), Reference(id=1320389056639385944, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[56], rfOrder=55, authorNames=null, journalName=null, refType=null, unstructuredReference=Papadopoulos I N, Jouhanneau J S, Takahashi N, et al. Dynamic conjugate F-SHARP microscopy[J]. Light Sci Appl, 2020, 9: 110., articleTitle=null, refAbstract=null), Reference(id=1320389056714883417, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[57], rfOrder=56, authorNames=null, journalName=null, refType=null, unstructuredReference=Demas J, Manley J, Tejera F, et al. High-speed, cortex-wide volumetric recording of neuroactivity at cellular resolution using light beads microscopy[J]. Nat Methods, 2021, 18: 1103-1111., articleTitle=null, refAbstract=null), Reference(id=1320389056773603674, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[58], rfOrder=57, authorNames=null, journalName=null, refType=null, unstructuredReference=Wu J, Liang Y, Chen S, et al. Kilohertz two-photon fluorescence microscopy imaging of neural activity in vivo[J]. Nat Methods, 2020, 17: 287-290., articleTitle=null, refAbstract=null), Reference(id=1320389056983318875, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[59], rfOrder=58, authorNames=null, journalName=null, refType=null, unstructuredReference=Li X, Li Y, Zhou Y, et al. Real-time denoising enables high-sensitivity fluorescence time-lapse imaging beyond the shotnoise limit[J]. Nat Biotechnol, 2023, 41: 282-292., articleTitle=null, refAbstract=null)], funds=[Fund(id=1320389048036868383, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, awardId=82371760, language=CN, fundingSource=国家自然科学基金面上项目(82371760), fundOrder=null, country=null)], companyList=[AuthorCompany(id=1320389043389579522, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, xref=null, ext=[AuthorCompanyExt(id=1320389043423133955, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, companyId=1320389043389579522, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=Shanghai Institute of Immunology, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China), AuthorCompanyExt(id=1320389043444105476, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, companyId=1320389043389579522, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=上海交通大学医学院上海市免疫学研究所，上海　200025)])], figs=null, attaches=null, journal=Journal(id=1319373034752147606, delFlag=0, nameCn=现代仪器与医疗, nameEn=Modern Instruments & Medical Treatment, nameHistory1=null, nameHistory2=null, issn=2095-5200, eissn=null, cn=10-1084/TH, coden=null, periodic=1, language=CN, oaType=null, ccby=null, superviseOffice=null, ownerOffice=null, pubOffice=null, editorOffice=null, officeType=null, aims=null, clcCode=null, officeProv=null, officeCity=null, officeAddr=null, officeZip=null, officeEmail=null, officePhone=null, editDirector=null, officeDirector=null, officeDirectorPhone=null, officeStaffNum=null, officeEmpNum=null, coverPicUrl=ndMvm7vxHT79E2yRWNKNzQ==, journalPrice=null, startedYear=null, abbrevIsoEn=Modern Instruments & Medical Treatment, journalRemark=null, publicationField=null, createdTime=1792492644252, updatedTime=1792494001447, createdBy=18614031015, updatedBy=13701087609, firstLetterCn=X, firstLetterEn=X, subjectCode=Medical and Pharmaceutical Sciences, subjectName=null, subjectCodeEn=Medical and Pharmaceutical Sciences, subjectNameEn=null, picCn=ndMvm7vxHT79E2yRWNKNzQ==, picEn=KKKOecvm1TyCdUk3lioBZw==, jcr=null, cjcr=null, exts=[JournalExt(id=1319378727318958500, language=CN, name=现代仪器与医疗, nameHistory1=null, nameHistory2=null, managedBy=, sponsoredBy=, publishedBy=, editorOffice=, officeProv=null, officeCity=null, officeAddr=, officeZip=, editDirector=, officeDirector=null, officePhone=null, coverPicUrl=null, journalRemark=, submitArticleUrl=null, websiteUrl=, createdTime=1792494001464, updatedTime=1792494001464, createdBy=13701087609, updatedBy=13701087609, submissionGuidelinesUrl=, submissionAuthorUrl=https://xdyi.cbpt.cnki.net/editor/, submissionEditorUrl=https://xdyi.cbpt.cnki.net/editor/, submissionReviewUrl=https://xdyi.cbpt.cnki.net/editor/, submissionCeEditorUrl=, submissionAeEditorUrl=, option={"copyright":""}), JournalExt(id=1319378727369290149, language=EN, name=Modern Instruments & Medical Treatment, nameHistory1=null, nameHistory2=null, managedBy=, sponsoredBy=, publishedBy=, editorOffice=, officeProv=null, officeCity=null, officeAddr=, officeZip=, editDirector=, officeDirector=null, officePhone=null, coverPicUrl=null, journalRemark=, submitArticleUrl=null, websiteUrl=, createdTime=1792494001476, updatedTime=1792494001476, createdBy=13701087609, updatedBy=13701087609, submissionGuidelinesUrl=, submissionAuthorUrl=https://xdyi.cbpt.cnki.net/editor/, submissionEditorUrl=https://xdyi.cbpt.cnki.net/editor/, submissionReviewUrl=https://xdyi.cbpt.cnki.net/editor/, submissionCeEditorUrl=, submissionAeEditorUrl=, option={"copyright":""})], databaseList=null, tenantJournalId=1319373289681944729, websiteList=[Website(id=1319376331830313888, webName=null, webTitle=null, webDomain=null, webCopyrigh=null, webIpcNo=null, seoTitle=null, seoKeywords=null, seoDescription=null, tenantJournalId=null, journalId=1319373289681944729, journalNameCn=null, journalNameEn=null, grayFlag=null, tenantId=1146029695717560320, platformId=null, journalGroupId=null, journalGroupNameCn=null, journalGroupNameEn=null, type=1, domain=https://castjournals.cast.org.cn/joweb/xdyqyyl/CN, language=CN, createTime=1792493430335, createBy=18614031015, updateTime=1792493771498, updateBy=18614031015, name=现代仪器与医疗-中文, tplId=1146099689490845704, title=现代仪器与医疗, delFlag=0, indexPage=/home, props=[WebsiteProps(id=1319377854559802284, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1319376331830313888, code=articleTextType, value=kx, createTime=1792493793382, updateTime=1792493793382, creator=18614031015, updator=18614031015), WebsiteProps(id=1319377854522053545, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1319376331830313888, code=banner, value=null, createTime=1792493793373, updateTime=1792493793373, creator=18614031015, updator=18614031015), WebsiteProps(id=1319377854597551023, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1319376331830313888, code=grayFlag, value=0, createTime=1792493793391, updateTime=1792493793391, creator=18614031015, updator=18614031015), WebsiteProps(id=1319377854509470632, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1319376331830313888, code=logo, value=https://castjournals.cast.org.cn/joweb/xdyqyyl/CN/file/pic?fileId=31v6dhjrP9Yy6tUJUZA+UQ==, createTime=1792493793370, updateTime=1792493793370, creator=18614031015, updator=18614031015), WebsiteProps(id=1319377854614328241, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1319376331830313888, code=minRunFlag, value=0, createTime=1792493793395, updateTime=1792493793395, creator=18614031015, updator=18614031015), WebsiteProps(id=1319377854538830763, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1319376331830313888, code=picServerUrl, value=https://castjournals.cast.org.cn/joweb/xdyqyyl/CN/file/pic, createTime=1792493793377, updateTime=1792493793377, creator=18614031015, updator=18614031015), WebsiteProps(id=1319377854605939632, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1319376331830313888, code=silenceFlag, value=0, createTime=1792493793393, updateTime=1792493793393, creator=18614031015, updator=18614031015), WebsiteProps(id=1319377854530442154, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1319376331830313888, code=staticResourcePath, value=https://castjournals.cast.org.cn/joweb/cast_kjdb_cn_619/, createTime=1792493793375, updateTime=1792493793375, creator=18614031015, updator=18614031015), WebsiteProps(id=1319377854568190893, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1319376331830313888, code=themeColor, value=null, createTime=1792493793384, updateTime=1792493793384, creator=18614031015, updator=18614031015), WebsiteProps(id=1319377854584968110, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1319376331830313888, code=themeStyle, value=null, createTime=1792493793388, updateTime=1792493793388, creator=18614031015, updator=18614031015)]), Website(id=1319376332157469608, webName=null, webTitle=null, webDomain=null, webCopyrigh=null, webIpcNo=null, seoTitle=null, seoKeywords=null, seoDescription=null, tenantJournalId=null, journalId=1319373289681944729, journalNameCn=null, journalNameEn=null, grayFlag=null, tenantId=1146029695717560320, platformId=null, journalGroupId=null, journalGroupNameCn=null, journalGroupNameEn=null, type=1, domain=https://castjournals.cast.org.cn/joweb/xdyqyyl/EN, language=EN, createTime=1792493430420, createBy=18614031015, updateTime=1792493768050, updateBy=18614031015, name=现代仪器与医疗-英文, tplId=1146101810881728533, title=Modern Instruments & Medical Treatment, delFlag=0, indexPage=/home, props=[WebsiteProps(id=1319377880879059898, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1319376332157469608, code=articleTextType, value=kx, createTime=1792493799657, updateTime=1792493799657, creator=18614031015, updator=18614031015), WebsiteProps(id=1319377880845505463, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1319376332157469608, code=banner, value=null, createTime=1792493799649, updateTime=1792493799649, creator=18614031015, updator=18614031015), WebsiteProps(id=1319377880921002941, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1319376332157469608, code=grayFlag, value=0, createTime=1792493799667, updateTime=1792493799667, creator=18614031015, updator=18614031015), WebsiteProps(id=1319377880832922550, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1319376332157469608, code=logo, value=https://castjournals.cast.org.cn/joweb/xdyqyyl/EN/file/pic?fileId=31v6dhjrP9Yy6tUJUZA+UQ==, createTime=1792493799646, updateTime=1792493799646, creator=18614031015, updator=18614031015), WebsiteProps(id=1319377880937780159, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1319376332157469608, code=minRunFlag, value=0, createTime=1792493799671, updateTime=1792493799671, creator=18614031015, updator=18614031015), WebsiteProps(id=1319377880866476985, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1319376332157469608, code=picServerUrl, value=https://castjournals.cast.org.cn/joweb/xdyqyyl/EN/file/pic, createTime=1792493799654, updateTime=1792493799654, creator=18614031015, updator=18614031015), WebsiteProps(id=1319377880929391550, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1319376332157469608, code=silenceFlag, value=0, createTime=1792493799669, updateTime=1792493799669, creator=18614031015, updator=18614031015), WebsiteProps(id=1319377880858088376, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1319376332157469608, code=staticResourcePath, value=https://castjournals.cast.org.cn/joweb/cast_kjdb_en_623/, createTime=1792493799652, updateTime=1792493799652, creator=18614031015, updator=18614031015), WebsiteProps(id=1319377880887448507, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1319376332157469608, code=themeColor, value=null, createTime=1792493799659, updateTime=1792493799659, creator=18614031015, updator=18614031015), WebsiteProps(id=1319377880900031420, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1319376332157469608, code=themeStyle, value=null, createTime=1792493799662, updateTime=1792493799662, creator=18614031015, updator=18614031015)])], journalTitle=现代仪器与医疗, weixinUrl=null, journalUrl=https://xdyi.cbpt.cnki.net/, iacademicId=null, status=1, seqNo=null, journalTitleEn=Modern Instruments & Medical Treatment, journalPhotoCn=ndMvm7vxHT79E2yRWNKNzQ==, journalPhotoEn=KKKOecvm1TyCdUk3lioBZw==, journalFirstLetter=X, journalRecommend=null, journalNew=null, journalCollection=null, jcrJf=null, cjcrJf=null, jcrJfStr=null, cjcrJfStr=null, submissionFirstDecision=null, sciSubjectClassification=null, casSubjectClassification=null, citeScore=null, totalCitationFrequency=null, icpCode=null, psCode=null, advertisingLicenseCode=null, copyrightInformation=null, country=null, option=, provinceCode=null, provinceName=null, collectFlag=false, interPubPlatform=, interPubPlatformUrl=null), detailUrlCn=https://castjournals.cast.org.cn/joweb/xdyqyyl/CN/10.11876/mimt202504012, detailUrlEn=https://castjournals.cast.org.cn/joweb/xdyqyyl/EN/10.11876/mimt202504012, pdfUrlCn=https://castjournals.cast.org.cn/joweb/xdyqyyl/CN/PDF/10.11876/mimt202504012, pdfUrlEn=https://castjournals.cast.org.cn/joweb/xdyqyyl/EN/PDF/10.11876/mimt202504012, aliStartDate=0, aliEndDate=0, collectionFlag=false, citedCount=null, citedUrl=null, previewStatus=0, delFlag=0, hasFullText=0, orderTime=1754755200000, fullTextJson=null, articleText=null, reference=null)
收藏切换
双光子活体成像在生命科学领域的应用与展望
收藏切换
PDF下载
柏文娟 , 王靳 , 赵文颖 , 王静 *
现代仪器与医疗 | 生物应用 2025,31(4): 111-116
收起
收藏切换
现代仪器与医疗 |生物应用 2025 , 31 (4) : 111 -116
双光子活体成像在生命科学领域的应用与展望
全屏
[Author(id=1320389043557351686, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, orderNo=0, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1320389045268627720, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, authorId=1320389043557351686, language=EN, stringName=Wenjuan Bai, firstName=Wenjuan, middleName=null, lastName=Bai, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=null, address=Shanghai Institute of Immunology, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1320389045436399881, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, authorId=1320389043557351686, language=CN, stringName=柏文娟, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=null, address=上海交通大学医学院上海市免疫学研究所，上海　200025, bio={"content":"

柏文娟，女，博士研究生，实验师，研究方向：主要从事显微成像平台、大型显微镜的管理工作

"}, bioImg=null, bioContent=

柏文娟，女，博士研究生，实验师，研究方向：主要从事显微成像平台、大型显微镜的管理工作

, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1320389043389579522, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, xref=null, ext=[AuthorCompanyExt(id=1320389043423133955, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, companyId=1320389043389579522, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=Shanghai Institute of Immunology, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China), AuthorCompanyExt(id=1320389043444105476, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, companyId=1320389043389579522, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=上海交通大学医学院上海市免疫学研究所，上海　200025)])]), Author(id=1320389045553840395, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, orderNo=1, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1320389045725806861, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, authorId=1320389045553840395, language=EN, stringName=Jin Wang, firstName=Jin, middleName=null, lastName=Wang, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=null, address=Shanghai Institute of Immunology, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1320389045851635982, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, authorId=1320389045553840395, language=CN, stringName=王靳, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=null, address=上海交通大学医学院上海市免疫学研究所，上海　200025, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1320389043389579522, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, xref=null, ext=[AuthorCompanyExt(id=1320389043423133955, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, companyId=1320389043389579522, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=Shanghai Institute of Immunology, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China), AuthorCompanyExt(id=1320389043444105476, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, companyId=1320389043389579522, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=上海交通大学医学院上海市免疫学研究所，上海　200025)])]), Author(id=1320389045960687888, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, orderNo=2, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1320389046115877138, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, authorId=1320389045960687888, language=EN, stringName=Wenying Zhao, firstName=Wenying, middleName=null, lastName=Zhao, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=null, address=Shanghai Institute of Immunology, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1320389046287843603, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, authorId=1320389045960687888, language=CN, stringName=赵文颖, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=null, address=上海交通大学医学院上海市免疫学研究所，上海　200025, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1320389043389579522, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, xref=null, ext=[AuthorCompanyExt(id=1320389043423133955, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, companyId=1320389043389579522, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=Shanghai Institute of Immunology, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China), AuthorCompanyExt(id=1320389043444105476, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, companyId=1320389043389579522, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=上海交通大学医学院上海市免疫学研究所，上海　200025)])]), Author(id=1320389046430449941, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, orderNo=3, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=1, authorType=1, ext={EN=AuthorExt(id=1320389046606610711, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, authorId=1320389046430449941, language=EN, stringName=Jing Wang, firstName=Jing, middleName=null, lastName=Wang, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=*, address=Shanghai Institute of Immunology, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1320389046753411352, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, authorId=1320389046430449941, language=CN, stringName=王静, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=*, address=上海交通大学医学院上海市免疫学研究所，上海　200025, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1320389043389579522, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, xref=null, ext=[AuthorCompanyExt(id=1320389043423133955, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, companyId=1320389043389579522, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=Shanghai Institute of Immunology, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China), AuthorCompanyExt(id=1320389043444105476, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, companyId=1320389043389579522, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=上海交通大学医学院上海市免疫学研究所，上海　200025)])])]
柏文娟, 王靳, 赵文颖, 王静*
作者信息
  • 上海交通大学医学院上海市免疫学研究所，上海　200025
通讯作者:
王静，女，博士研究生，研究员，研究方向：运用活体成像研究天然免疫细胞的动态及功能
作者简介:

柏文娟，女，博士研究生，实验师，研究方向：主要从事显微成像平台、大型显微镜的管理工作

Applications and prospects of two-photon intravital imaging in the field of life sciences
Wenjuan Bai, Jin Wang, Wenying Zhao, Jing Wang*
Affiliations
  • Shanghai Institute of Immunology, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China
出版时间: 2025-08-10 doi: 10.11876/mimt202504012
文章导航
收藏切换
摘要
收起

双光子活体成像技术具有深层成像的优势，可以直观地监测活体的体内动态过程，已成为生命科学领域研究的重要手段。本文简要介绍了双光子显微镜的技术原理，综述了双光子显微镜在生命科学研究中的应用领域，重点分析了双光子显微活体成像技术的发展趋势，探讨其当前面临的挑战及未来的优化方向，旨在为进一步优化双光子活体成像技术，推动双光子活体成像技术更加广泛的应用提供参考。

活体成像  /  双光子显微镜  /  生命科学

Intravital two-photon imaging technology has the advantage of deep imaging, enabling the direct monitoring of the dynamic processes within living organisms. It has become an important research tool in the field of life sciences. We briefly explain the basic principles of two-photon microscopy and its applications in life science research in recent years. We mainly focuse on analyzing the development trends of intravital two-photon imaging technology and discuss the current challenges and future optimization directions. We look forward to the continuous optimization of intravital two-photon imaging technology, which can promote its wider application.

Intravital imaging  /  Two-photon microscope  /  Life Sciences
柏文娟, 王靳, 赵文颖, 王静. 双光子活体成像在生命科学领域的应用与展望. 现代仪器与医疗, 2025 , 31 (4) : 111 -116 . DOI: 10.11876/mimt202504012
Wenjuan Bai, Jin Wang, Wenying Zhao, Jing Wang. Applications and prospects of two-photon intravital imaging in the field of life sciences[J]. Modern Instruments & Medical Treatment, 2025 , 31 (4) : 111 -116 . DOI: 10.11876/mimt202504012
基金
收起
  • 国家自然科学基金面上项目(82371760)
文献
收起
参考文献 引证文献
排序方式：
[1]
Denk W, Strickler J H, Webb W W. Two-photon laser scanning fluorescence microscopy[J]. Science, 1990, 248(4951): 73-76.
[2]
Kuan A T, Bondanelli G, Driscoll L N, et al. Synaptic wiring motifs in posterior parietal cortex support decision-making[J]. Nature, 2024, 627: 367-373.
[3]
Shainer I, Kappel J M, Laurell E, et al. Transcriptomic neuron types vary topographically in function and morphology[J]. Nature, 2025, 638: 1023-1033.
[4]
Drieu C, Zhu Z, Wang Z, et al. Rapid emergence of latent knowledge in the sensory cortex drives learning[J]. Nature, 2025, 641: 960-970.
[5]
Sun W, Winnubst J, Natrajan M, et al. Learning produces an orthogonalized state machine in the hippocampus[J]. Nature, 2025, 640: 165-175.
[6]
Xiong H, Tang F, Guo Y, et al. Neural circuit changes in neurological disorders: evidence from in vivo two-photon imaging[J]. Ageing Res Rev, 2023, 87: 101933.
[7]
Haage T R, Charakopoulos E, Bhuria V, et al. Neutrophil-specific expression of JAK2-V617F or CALRmut induces distinct inflammatory profiles in myeloproliferative neoplasia[J]. J Hematol Oncol, 2024, 17: 43.
[8]
Cheng L, Liu W, Zhong C, et al. Remodeling the homeostasis of pro- and anti-angiogenic factors by Shenmai injection to normalize tumor vasculature for enhanced cancer chemotherapy[J]. J Ethnopharmacol, 2021, 270: 113770.
[9]
Schoofs H, Daubel N, Schnabellehner S, et al. Dynamic cytoskeletal regulation of cell shape supports resilience of lymphatic endothelium[J]. Nature, 2025, 641: 465-475.
[10]
Pae J, Schwan N, Ottino-Loffler B, et al. Transient silencing of hypermutation preserves B cell affinity during clonal bursting[J]. Nature, 2025, 641: 486-494.
[11]
Wu T, Liao J, Xiang F, et al. Short-wavelength excitation two-photon intravital microscopy of endogenous fluorophores[J]. Biomed Opt Express, 2023, 14(7): 3380-3396.
[12]
Wang Y, Heymann F, Peiseler M. Intravital imaging: dynamic insights into liver immunity in health and disease[J]. Gut, 2024, 73(8): 1364-1375.
[13]
Liu D, Winer B Y, Chou M Y, et al. Dynamic encounters with red blood cells trigger splenic marginal zone B cell retention and function[J]. Nat Immunol, 2024, 25(1): 142-154.
[14]
Gaertner F, Ishikawa-Ankerhold H, Stutte S, et al. Plasmacytoid dendritic cells control homeostasis of megakaryopoiesis[J]. Nature, 2024, 631: 645-653.
[15]
Asquith N L, Carminita E, Camacho V, et al. The bone marrow is the primary site of thrombopoiesis[J]. Blood, 2024, 143(3): 272-278.
[16]
Santocki M, Such A, Drab D, et al. NETs persisting in vasculature undergo self-renewal with consequences for subsequent infection: a mouse model study[J]. Blood, 2025, 145(18): 2070-2085.
[17]
van de Wall S, Anthony S M, Hancox L S, et al. Dynamic landscapes and protective immunity coordinated by influenza-specific lung-resident memory CD8+ T cells revealed by intravital imaging[J]. Immunity, 2024, 57(8): 1878-1892.
[18]
Uderhardt S, Neag G, Germain R N. Dynamic multiplex tissue imaging in inflammation research[J]. Annu Rev Pathol, 2024, 19: 43-67.
[19]
Zhu X, Tang Y, Chen J, et al. Monitoring wound healing of elastic cartilage using multiphoton microscopy[J]. Osteoarthritis and Cartilage, 2013, 21(11): 1799-1806.
[20]
Vicanolo T, Özcan A, Li J L, et al. Matrix-producing neutrophils populate and shield the skin[J]. Nature, 2025, 641: 740-748.
[21]
Pappinen S, Pryazhnikov E, Khiroug L, et al. Organotypic cell cultures and two-photon imaging: tools for in vitro and in vivo assessment of percutaneous drug delivery and skin toxicity[J]. Journal of Controlled Release, 2012, 161(2): 656-667.
[22]
Di Martino J S, Nobre A R, Mondal C, et al. A tumor-derived type III collagen-rich ECM niche regulates tumor cell dormancy[J]. Nat Cancer, 2022, 3: 90-107.
[23]
Dalla E, Papanicolaou M, Park M D, et al. Lung-resident alveolar macrophages regulate the timing of breast cancer metastasis[J]. Cell, 2024, 187(23): 6631-6648.
[24]
Isshiki Y, Chen X, Teater M, et al. EZH2 inhibition enhances T cell immunotherapies by inducing lymphoma immunogenicity and improving T cell function[J]. Cancer Cell, 2025, 43(1): 49-68.
[25]
Kim H R, Park J S, Park J H, et al. Cell-permeable transgelin-2 as a potent therapeutic for dendritic cell-based cancer immunotherapy[J]. J Hematol Oncol, 2021, 14(1): 43.
[26]
Wu Y X, Zhang D, Hu X, et al. Multicolor two-photon nanosystem for multiplexed intracellular imaging and targeted cancer therapy[J]. Angew Chem Int Ed Engl, 2021, 60(22): 12569-12576.
[27]
Fang F, Yuan Y, Wan Y, et al. Near-infrared thermally activated delayed fluorescence nanoparticle: a metal-free photosensitizer for two-photon-activated photodynamic therapy at the cell and small animal levels[J]. Small, 2022, 18(6): e2106215.
[28]
Wei W, Hao M, Zhou K, et al. In situ multimodal transparent electrophysiological hydrogel for in vivo miniature two-photon neuroimaging and electrocorticogram analysis[J]. Acta Biomater, 2022, 152: 86-99.
[29]
Liu Z, Lu X, Villette V, et al. Sustained deep-tissue voltage recording using a fast indicator evolved for two-photon microscopy[J]. Cell, 2022, 185(18): 3408-3425.
[30]
Waters J. A large field of view 2- and 3-photon microscope[J]. Light Sci Appl, 2025, 14(1): 106.
[31]
Choe K, Hontani Y, Wang T, et al. Intravital three-photon microscopy allows visualization over the entire depth of mouse lymph nodes[J]. Nat Immunol, 2022, 23(2): 330-340.
[32]
Zhao Z, Zhou Y, Liu B, et al. Two-photon synthetic aperture microscopy for minimally invasive fast 3D imaging of native subcellular behaviors in deep tissue[J]. Cell, 2023, 186(11): 2475-2491.
[33]
Olesiak-Banska J, Waszkielewicz M, Obstarczyk P, et al. Twophoton absorption and photoluminescence of colloidal gold nanoparticles and nanoclusters[J]. Chem Soc Rev, 2019, 48(15): 4087-4117.
[34]
Lu J, Li Z, Lu M, et al. Assessing early atherosclerosis by detecting and imaging of hypochlorous acid and phosphorylation using fluorescence nanoprobe[J]. Adv Mater, 2023, 35(52): e2307008.
[35]
Hong S T, Kim T H, Choi J W, et al. Two-photon probes for pH: detection of human colon cancer using two-photon microscopy[J]. Anal Chem, 2017, 89(18): 9830-9835.
[36]
Larson D R, Zipfel W R, Williams R M, et al. Water-soluble quantum dots for multiphoton fluorescence imaging in vivo[J]. Science, 2003, 300(5624): 1434-1436.
[37]
Juvekar V, Park S J, Yoon J, et al. Recent progress in the two-photon fluorescent probes for metal ions[J]. Coordination Chemistry Reviews, 2021, 427: 213574.
[38]
Mann K, Deny S, Ganguli S, et al. Coupling of activity, metabolism and behaviour across the Drosophila brain[J]. Nature, 2021, 593: 244-248.
[39]
Kagan T, Gabay M, Meenakshisundaram A, et al. Genetically encoded biosensor for fluorescence lifetime imaging of PTEN dynamics in the intact brain[J]. Nat Methods, 2025, 22: 764-777.
[40]
Zong W, Wu R, Li M, et al. Fast high-resolution miniature two-photon microscopy for brain imaging in freely behaving mice[J]. Nat Methods, 2017, 14: 713-719.
[41]
Zong W, Wu R, Chen S, et al. Miniature two-photon microscopy for enlarged field-of-view, multi-plane and long-term brain imaging[J]. Nat Methods, 2021, 18: 46-49.
[42]
Zong W, Obenhaus H A, Skytøen E R, et al. Large-scale two-photon calcium imaging in freely moving mice[J]. Cell, 2022, 185(7): 1240-1256.
[43]
Gu X, Zhao Z, Chen X, et al. Imaging microglia surveillance during sleep-wake cycles in freely behaving mice[J]. eLife, 2023, 12: RP86749.
[44]
Cheng N, Dong Q, Zhang Z, et al. Egocentric processing of items in spines, dendrites, and somas in the retrosplenial cortex[J]. Neuron, 2024, 112(4): 646-660.
[45]
Zhao Z, Zeng F, Wang H, et al. Encoding of social novelty by sparse GABAergic neural ensembles in the prelimbic cortex[J]. Sci Adv, 2022, 8(35): eabo4884.
[46]
Zhang Z, Jiang S, Shi K, et al. Visualizing seizure propagation in freely-moving mice via miniature two-photon microscopy[J]. Neurosci Bull, 2022, 38: 1593-1597.
[47]
Ueki H, Wang I H, Zhao D, et al. Multicolor two-photon imaging of in vivo cellular pathophysiology upon influenza virus infection using the two-photon IMPRESS[J]. Nat Protoc, 2020, 15: 1041-1065.
[48]
Song X, Guo Y, Chen C, et al. A silent two-photon imaging system for studying in vivo auditory neuronal functions[J]. Light Sci Appl, 2022, 11: 96.
[49]
Papagiakoumou E, Ronzitti E, Emiliani V. Scanless two-photon excitation with temporal focusing[J]. Nat Methods, 2020, 17: 571-581.
[50]
Platisa J, Ye X, Ahrens A M, et al. High-speed low-light in vivo two-photon voltage imaging of large neuronal populations[J]. Nat Methods, 2023, 20: 1095-1103.
[51]
Beaulieu D R, Davison I G, Kılıç K, et al. Simultaneous multiplane imaging with reverberation two-photon microscopy[J]. Nat Methods, 2020, 17: 283-286.
[52]
Lanore F, Cayco-Gajic N A, Gurnani H, et al. Cerebellar granule cell axons support high-dimensional representations[J]. Nat Neurosci, 2021, 24: 1142-1150.
[53]
Griffiths V A, Valera A M, Lau J Y, et al. Real-time 3D movement correction for two-photon imaging in behaving animals[J]. Nat Methods, 2020, 17: 741-748
[54]
Mok A T, Wang T, Zhao S, et al. A large field-of-view, single-cell-resolution two- and three-photon microscope for deep and wide imaging[J]. eLight, 2024, 4: 20.
[55]
Qin Z, He S, Yang C, et al. Adaptive optics two-photon microscopy enables near-diffraction-limited and functional retinal imaging in vivo[J]. Light Sci Appl, 2020, 9: 79.
[56]
Papadopoulos I N, Jouhanneau J S, Takahashi N, et al. Dynamic conjugate F-SHARP microscopy[J]. Light Sci Appl, 2020, 9: 110.
[57]
Demas J, Manley J, Tejera F, et al. High-speed, cortex-wide volumetric recording of neuroactivity at cellular resolution using light beads microscopy[J]. Nat Methods, 2021, 18: 1103-1111.
[58]
Wu J, Liang Y, Chen S, et al. Kilohertz two-photon fluorescence microscopy imaging of neural activity in vivo[J]. Nat Methods, 2020, 17: 287-290.
[59]
Li X, Li Y, Zhou Y, et al. Real-time denoising enables high-sensitivity fluorescence time-lapse imaging beyond the shotnoise limit[J]. Nat Biotechnol, 2023, 41: 282-292.
2025年第31卷第4期
PDF下载
64
9
引用本文
BibTeX
文章信息
doi: 10.11876/mimt202504012
  • 首发时间：2026-10-23
  • 出版时间：2025-08-10
补充材料
相关文章
文章信息
作者
出版历史
基金
国家自然科学基金面上项目(82371760)
作者信息
    上海交通大学医学院上海市免疫学研究所，上海　200025

通讯作者:

王静，女，博士研究生，研究员，研究方向：运用活体成像研究天然免疫细胞的动态及功能
参考文献
分享链接
https://castjournals.cast.org.cn/joweb/xdyqyyl/CN/10.11876/mimt202504012
分享至
全文二维码

扫描看全文

引用本文
BibTeX
本文的引用情况
2种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
关闭全屏
相关链接
论文
最新文章
热读文章
热点专题
内参
蓝皮书
产业发展报告
传播力报告
期刊分级目录
资讯
学术新闻
行业新闻
学术会议
行业会议
关于
关于我们
联系我们
北京市海淀区学院南路86号
100081
010-62662699（运营及技术支持）
qkjq@cast.org.cn

中国科学技术协会版权所有 京ICP 备10216604号-4 海淀分局备案 1101084647
科技导报社主办 中国科协信息中心技术支持