Article(id=1320388944148157335, tenantId=1146029695717560320, journalId=1319373289681944729, issueId=1320388933154878311, articleNumber=null, orderNo=null, doi=10.11876/mimt202504002, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=null, receivedDateStr=null, revisedDate=null, revisedDateStr=null, acceptedDate=null, acceptedDateStr=null, onlineDate=1792734855924, onlineDateStr=2026-10-23, pubDate=1754755200000, pubDateStr=2025-08-10, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1792734855924, onlineIssueDateStr=2026-10-23, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1792734855924, creator=13701087609, updateTime=1792734855924, updator=13701087609, issue=Issue{id=1320388933154878311, tenantId=1146029695717560320, journalId=1319373289681944729, year='2025', volume='31', issue='4', pageStart='2', pageEnd='132', issueExtLink='null', onlineDate='null', pubDate='1754755200000', pubDateStr='2025-08-10', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1792734853304, creator='13701087609', updateTime=1792746408152, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1320437397758791917, tenantId=1146029695717560320, journalId=1319373289681944729, issueId=1320388933154878311, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1320437397758791918, tenantId=1146029695717560320, journalId=1319373289681944729, issueId=1320388933154878311, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=16, endPage=21, ext={EN=ArticleExt(id=1320388944328512408, articleId=1320388944148157335, tenantId=1146029695717560320, journalId=1319373289681944729, language=EN, title=Research progress on multiscale, high-speed, and low-phototoxicity light sheet microscopy, columnId=null, journalTitle=Modern Instruments & Medical Treatment, columnName=null, runingTitle=null, highlight=null, articleAbstract=

Fluorescence microscopy has become an indispensable tool for visualizing subcellular structures and dynamic biological processes. Light sheet fluorescence microscopy (LSFM), characterized by selective plane illumination and detection, has attracted increasing attention in recent years owing to its low phototoxicity, high spatiotemporal resolution, and large field of view. The emergence of single-objective light sheet systems has further enhanced sample compatibility and system flexibility, opening new avenues for multiscale, long-term, and three-dimensional dynamic imaging. Here, we review the development and diverse configurations of LSFM, with a particular focus on the technical principles, representative applications, and performance advantages of single-objective systems. We also discuss their current bottlenecks in terms of field of view, optical path complexity, and signal transmission efficiency, and explore the potential of zoom-free multiscale single-objective platforms that integrate structural innovations, multimodal imaging, and intelligent control, aiming to provide a reference for the creation of high-performance, bio-friendly, and broadly applicable imaging systems.

, authors=Yifan Ma¹, Yuxuan Zhao¹, Fang Zhao¹, Meiling Chu², Peng Fei^{1, *}, authorsList=Yifan Ma, Yuxuan Zhao, Fang Zhao, Meiling Chu, Peng Fei, authorCompany=null, correspAuthors=Peng Fei, authorNote=null, correspAuthorsNote=null, copyrightStatement=All copyrights in 2025 belong to the sponsor of the journal.Not content published by the journal may be reproduced or abridged without authorization., copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1320388944613725081, articleId=1320388944148157335, tenantId=1146029695717560320, journalId=1319373289681944729, language=CN, title=多尺度、高速、低光毒性光片荧光显微技术研究进展, columnId=1320388935667266410, journalTitle=现代仪器与医疗, columnName=技术进展, runingTitle=null, highlight=null, articleAbstract=

荧光显微镜作为揭示细胞亚结构与生命活动动态过程的关键工具，已成为生命科学研究中不可或缺的一环。光片荧光显微镜（light sheet fluorescence microscopy，LSFM）作为一种以选择性平面激发和探测为特征的先进成像技术，凭借其低光毒性、高时空分辨率和大视野等优势，近年来在生命科学与医学研究领域获得广泛关注。特别是单物镜光片系统的出现，进一步提高了样品兼容性与系统灵活性，为多尺度、长时程及三维动态成像提供了新途径。本文系统回顾了LSFM的发展历程与多样化构型，重点综述单物镜系统的技术原理、典型应用与性能优势，分析其在视野、光路复杂度和信号传输效率等方面的瓶颈，并探讨无需变倍的多尺度单物镜光片成像系统在结构创新、多模态融合及智能成像控制方面的发展潜力，为构建高性能、生物友好型、通用化成像平台提供参考。

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马异凡，男，在读博士研究生，研究方向：计算光学显微成像

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马异凡，男，在读博士研究生，研究方向：计算光学显微成像

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