现代仪器与医疗

基于分子印迹的电化学探针在临床检测25-羟基维生素D₃中的性能探究

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李嘉伟 , 徐立新 , 郎春辉 ^*

现代仪器与医疗 | 医学工程 2025,31(3): 17-23

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现代仪器与医疗 |医学工程 2025 , 31 (3) : 17 -23

基于分子印迹的电化学探针在临床检测25-羟基维生素D₃中的性能探究

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李嘉伟, 徐立新, 郎春辉^*

作者信息

重庆大学附属三峡医院，重庆市老年疾病临床医学研究中心，重庆　404000

通讯作者:

郎春辉，男，博士研究生，副主任医师，研究方向：生物医药

作者简介:

李嘉伟，男，博士研究生，副研究员，研究方向：微纳传感材料

Investigation of the performance of molecularly imprinted electrochemical probes in clinical detection of 25-(OH)D₃

Jiawei Li, Lixin Xu, Chunhui Lang^*

Affiliations

Chongqing Municipality Clinical Research Center for Geriatric Diseases, Chongqing University Three Gorges Hospital, Chongqing 404000, China

出版时间: 2025-06-10 doi: 10.11876/mimt202503003

文章导航

摘要

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目的

开发一种基于分子印迹技术（molecularly imprinted polymers，MIPs）的高灵敏度、高选择性电化学探针，用于25-羟基维生素D₃[25-（OH）D₃]的精准检测，以满足维生素D代谢相关疾病的诊断需求。

方法

通过逐层修饰丝网印刷电极（screen-printed electrode，SPE），构建Co₃O₄@还原氧化石墨烯（reduced graphene oxide，rGO）@Pt@Au复合基底，以增强导电性。结合聚吡咯的电沉积与模板分子洗脱，成功形成了具有特异性识别能力的印迹空腔。通过优化扫描速率、沉积循环次数、孵育时间和温度，平衡了膜层结构、电子传递效率与特异性结合能力。采用方波伏安法（square wave voltammetry，SWV）对该传感器的响应性能进行评估，分析其灵敏度、选择性及重现性。

结果

扫描电子显微镜（scanning electron microscope，SEM）表征显示，模板洗脱后电极表面形成清晰的印迹空腔，该空腔可在100 nmol/L至1 mmol/L范围内进行靶标检测，其检测限为26.76 nmol/L。该检测策略对10倍浓度干扰物无特异性响应，且3批次电极重现性相对标准偏差为3.2%。加标实验也显示该检测策略具有较好的实际应用能力。

结论

本研究成功构建了一种灵敏度高、抗干扰性强、重现性优异的新方法，为临床上25-（OH）D₃的检测提供了一种新思路。

关键词

25-羟基维生素D₃ / 分子印迹聚合物 / 电化学检测1

Abstract

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Objective

To develop a highly sensitive and selective electrochemical probe based on molecular imprinting polymers (MIPs) technology for the precise detection of 25-hydroxyvitamin D₃ [25-(OH)D₃], aiming to meet the diagnostic needs of vitamin D metabolism-related diseases.

Methods

The Co₃O₄@reduced graphene oxide (rGO)@Pt@Au composite substrate was constructed by layer-by-layer modification of screen-printed electrode (SPE) to enhance conductivity. Molecularly imprinted cavities were successfully formed through electropolymerization of polypyrrole and template molecule elution, imparting specific recognition capabilities. By optimizing the scan rate, deposition cycles, incubation time, and temperature, the balance between membrane structure, electron transfer efficiency, and specific binding ability was achieved. The sensor's response performance was evaluated using square wave voltammetry (SWV), and its sensitivity, selectivity, and reproducibility were analyzed.

Results

Scanning electron microscopy (SEM) characterization revealed that after template elution, clear imprinted cavities were formed on the electrode surface, which could detect the target in the range of 100 nmol/L to 1 mmol/L, with a detection limit of 26.76 nmol/L. This detection strategy showed no specific response to interference at 10 times the concentration, and the reproducibility of the electrodes across three batches, with a relative standard deviation of 3.2%, indicated the reliability of the method. The spiked experiments also show its good practical application ability.

Conclusion

This study successfully established a new method with high sensitivity, strong anti-interference ability, and excellent reproducibility, providing a novel approach for the clinical detection of 25-(OH)D₃.

Key words

25-hydroxyvitamin D₃ / Molecularly imprinted polymers / Electrochemical detection

引用本文

李嘉伟, 徐立新, 郎春辉. 基于分子印迹的电化学探针在临床检测25-羟基维生素D₃中的性能探究. 现代仪器与医疗, 2025 , 31 (3) : 17 -23 . DOI: 10.11876/mimt202503003

Jiawei Li, Lixin Xu, Chunhui Lang. Investigation of the performance of molecularly imprinted electrochemical probes in clinical detection of 25-(OH)D₃[J]. Modern Instruments & Medical Treatment, 2025 , 31 (3) : 17 -23 . DOI: 10.11876/mimt202503003

基金

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重庆市万州区博士“直通车”科研项目(wzstc-20220134)
国家自然科学基金资助项目(82402741)
重庆大学附属三峡医院科研项目(2022YJKYXM-034)

参考文献

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2025年第31卷第3期

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文章信息

doi: 10.11876/mimt202503003

首发时间：2026-10-23
出版时间：2025-06-10

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基金

重庆市万州区博士“直通车”科研项目(wzstc-20220134)

国家自然科学基金资助项目(82402741)

重庆大学附属三峡医院科研项目(2022YJKYXM-034)

作者信息

重庆大学附属三峡医院，重庆市老年疾病临床医学研究中心，重庆　404000

通讯作者:

郎春辉，男，博士研究生，副主任医师，研究方向：生物医药

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2种不同金属材料的力学参数

科 Family	属数 Number of genus	种数 Number of species	占总种数比例 Percentage of total species (%)	属 Genus	种数 Number of species	占总种数比例 Percentage of total species (%)
鹅膏菌科Amanitaceae	2	11	5.26	鹅膏菌属 Amanita	10	4.78
小菇科 Mycenaceae	2	12	5.74	丝盖伞属 Inocybe	5	2.39
多孔菌科 Polyporaceae	8	14	6.70	蜡蘑属 Laccaria	5	2.39
红菇科 Russulaceae	3	23	11.00	小皮伞属 Marasmius	6	2.87
				小菇属 Mycena	11	5.26
				光柄菇属 Pluteus	5	2.39
				红菇属 Russula	17	8.13
				栓菌属 Trametes	5	2.39

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