现代仪器与医疗

锰基纳米平台协同多疗法增强肝癌治疗的研究

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闫康宁 , 刘军杰 ^*

现代仪器与医疗 | 临床研究 2025,31(1): 51-57

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现代仪器与医疗 |临床研究 2025 , 31 (1) : 51 -57

锰基纳米平台协同多疗法增强肝癌治疗的研究

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闫康宁, 刘军杰^*

作者信息

广西医科大学附属肿瘤医院超声科，南宁　530000

通讯作者:

刘军杰，女，博士研究生，研究员，研究方向：肿瘤纳米药物治疗

作者简介:

闫康宁，男，硕士研究生，住院医师，研究方向：肿瘤纳米药物治疗

Manganese-based nanoplatforms for enhanced hepatocellular carcinoma therapy

Kangning Yan, Junjie Liu^*

Affiliations

Department of Medical Ulrasound, Guangxi Medical University Cancer Hospital, Nanning 530000, China

出版时间: 2025-02-10 doi: 10.11876/mimt202501009

文章导航

摘要

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目的

本研究旨在开发一种负载Mn²⁺的聚多巴胺纳米平台（Mn-PDA，MD），通过光热疗法联合化学动力疗法，探讨其在肝细胞癌治疗中的机制与效果。

方法

采用自氧化聚合法合成MD，并通过透射电子显微镜、动态光散射及X射线光电子能谱表征其结构和性质。进一步评估其光热性能、Mn²⁺的响应释放特性以及类芬顿反应催化活性。在体外实验中，通过CCK-8和活/死染色评估肿瘤细胞存活率，并检测光热效应和Mn²⁺释放对细胞毒性的影响。

结果

本研究合成的MD粒径约200nm，经近红外（Near-Infrared，NIR）激光照射7min，温度迅速升至47.2℃。NIR照射触发MD释放Mn²⁺，进一步催化生成羟基自由基。体外实验显示，在100μg/ml和NIR照射（1W/cm²，10min）条件下，MD将肿瘤细胞活力降低至40%。活/死细胞染色进一步证实，大量肿瘤细胞发生死亡。与聚多巴胺相比，MD表现出更显著的杀伤能力，差异具有统计学意义。

结论

本研究开发的MD通过光热效应与Mn²⁺介导的活性氧的产生，实现了对肿瘤细胞的高效杀伤，具有协同治疗的优势，为肝癌治疗提供了新的策略和方向。

关键词

纳米材料 / 光热疗法 / 化学动力学疗法 / 锰离子 / 肝细胞癌

Abstract

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Objective

This study aims to develop a polydopamine-based nanoplatform loaded with Mn²⁺ (Mn-PDA, MD) and explore its mechanism and efficacy in hepatocellular carcinoma treatment through the combination of photothermal therapy and chemodynamic therapy.

Methods

MD was synthesized by self-oxidative polymerization and characterized by transmission electron microscopy, dynamic light scattering, and X-ray photoelectron spectroscopy for its structure and properties. Its photothermal performance, Mn²⁺ release characteristics in response to stimuli, and Fenton-like reaction catalytic activity were further evaluated. In vitro experiments were conducted to assess the survival rate of tumor cells using CCK-8 and live/dead staining, and to detect the effects of photothermal effect and Mn²⁺ release on cytotoxicity.

Results

The synthesized MD had a particle size of approximately 200 nm. After 7 minutes of near-infrared (NIR) laser irradiation (1 W/cm²), the temperature rapidly rose to 47.2℃. NIR irradiation triggered the release of Mn²⁺ from MD, which further catalyzed the generation of hydroxyl radicals. In vitro experiments showed that at a concentration of 100 μg/ml and under NIR irradiation (1 W/cm², 10 min), MD reduced the viability of tumor cells to 40%. Live/dead staining further confirmed that a large number of tumor cells died. Compared with polydopamine, MD exhibited more significant killing ability, and the difference was statistically significant.

Conclusion

The developed MD achieved efficient killing of tumor cells through the combination of photothermal effect and Mn²⁺-mediated reactive oxygen species generation, demonstrating the advantages of synergistic therapy and providing a new strategy and direction for liver cancer treatment.

Key words

Nanomaterials / Photothermal therapy / Chemodynamic therapy / Manganese-ion / Hepatocellular carcinoma

引用本文

闫康宁, 刘军杰. 锰基纳米平台协同多疗法增强肝癌治疗的研究. 现代仪器与医疗, 2025 , 31 (1) : 51 -57 . DOI: 10.11876/mimt202501009

Kangning Yan, Junjie Liu. Manganese-based nanoplatforms for enhanced hepatocellular carcinoma therapy[J]. Modern Instruments & Medical Treatment, 2025 , 31 (1) : 51 -57 . DOI: 10.11876/mimt202501009

基金

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广西影像医学临床医学研究中心建设(桂科AD20238096)

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2025年第31卷第1期

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文章信息

doi: 10.11876/mimt202501009

首发时间：2026-10-23
出版时间：2025-02-10

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基金

广西影像医学临床医学研究中心建设(桂科AD20238096)

作者信息

广西医科大学附属肿瘤医院超声科，南宁　530000

通讯作者:

刘军杰，女，博士研究生，研究员，研究方向：肿瘤纳米药物治疗

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2种不同金属材料的力学参数

科 Family	属数 Number of genus	种数 Number of species	占总种数比例 Percentage of total species (%)	属 Genus	种数 Number of species	占总种数比例 Percentage of total species (%)
鹅膏菌科Amanitaceae	2	11	5.26	鹅膏菌属 Amanita	10	4.78
小菇科 Mycenaceae	2	12	5.74	丝盖伞属 Inocybe	5	2.39
多孔菌科 Polyporaceae	8	14	6.70	蜡蘑属 Laccaria	5	2.39
红菇科 Russulaceae	3	23	11.00	小皮伞属 Marasmius	6	2.87
				小菇属 Mycena	11	5.26
				光柄菇属 Pluteus	5	2.39
				红菇属 Russula	17	8.13
				栓菌属 Trametes	5	2.39

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