Article(id=1320389090273510031, tenantId=1146029695717560320, journalId=1319373289681944729, issueId=1320388933154878311, articleNumber=null, orderNo=null, doi=10.11876/mimt202504014, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=null, receivedDateStr=null, revisedDate=null, revisedDateStr=null, acceptedDate=null, acceptedDateStr=null, onlineDate=1792734890763, onlineDateStr=2026-10-23, pubDate=1754755200000, pubDateStr=2025-08-10, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1792734890763, onlineIssueDateStr=2026-10-23, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1792734890763, creator=13701087609, updateTime=1792734890763, updator=13701087609, issue=Issue{id=1320388933154878311, tenantId=1146029695717560320, journalId=1319373289681944729, year='2025', volume='31', issue='4', pageStart='2', pageEnd='132', issueExtLink='null', onlineDate='null', pubDate='1754755200000', pubDateStr='2025-08-10', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1792734853304, creator='13701087609', updateTime=1792746408152, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1320437397758791917, tenantId=1146029695717560320, journalId=1319373289681944729, issueId=1320388933154878311, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1320437397758791918, tenantId=1146029695717560320, journalId=1319373289681944729, issueId=1320388933154878311, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=126, endPage=132, ext={EN=ArticleExt(id=1320389090441282192, articleId=1320389090273510031, tenantId=1146029695717560320, journalId=1319373289681944729, language=EN, title=Sample preparation and operating methods for two-photon intravital imaging, columnId=null, journalTitle=Modern Instruments & Medical Treatment, columnName=null, runingTitle=null, highlight=null, articleAbstract=

Intravital two-photon imaging technology has become an essential tool in life science research due to its ability to intuitively monitor the in vivo dynamic processes of living organisms. However, sample preparation has always been a major challenge in the application of this technology, and it directly determines the stability and quality of imaging. There is currently no systematic introduction to sample preparation for intravital two-photon imaging. Based on the rich practical experience accumulated in the laboratory over the years in the preparation of samples for intravital imaging, we mainly introduce the sample preparation procedures and key points of operation for tissues such as the lung, intestine, brain, liver, and skin. The aim is to provide a reference for sample preparation and operation before intravital two-photon imaging, assist researchers in mastering the operation of intravital two-photon imaging, and promote the broader application of this technology.

, authors=Wenjuan Bai, Jin Wang, Wenying Zhao, Jing Wang^*, authorsList=Wenjuan Bai, Jin Wang, Wenying Zhao, Jing Wang, authorCompany=null, correspAuthors=Jing Wang, authorNote=null, correspAuthorsNote=null, copyrightStatement=All copyrights in 2025 belong to the sponsor of the journal.Not content published by the journal may be reproduced or abridged without authorization., copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1320389090822963858, articleId=1320389090273510031, tenantId=1146029695717560320, journalId=1319373289681944729, language=CN, title=双光子活体成像样本准备及操作方法研究, columnId=1320389090516779665, journalTitle=现代仪器与医疗, columnName=样本制备, runingTitle=null, highlight=null, articleAbstract=

双光子活体成像技术能够直观地监测活体的体内动态过程，已成为生命科学研究的重要手段，然而样本准备一直是该技术运用的难点，样本准备直接决定了成像的稳定性和成像质量。鉴于目前关于双光子活体成像的样本准备尚无系统性的介绍，本文结合实验室多年来在活体成像样本准备方面的实践积累，重点介绍肺脏、肠道、脑、肝脏、皮肤等组织的样本准备流程与上机操作要点，旨在为双光子活体成像的样本准备及上机操作提供参考，帮助研究人员掌握双光子活体成像的操作技巧，推进双光子活体成像技术更加广泛的运用。

, authors=柏文娟, 王靳, 赵文颖, 王静^*, authorsList=柏文娟, 王靳, 赵文颖, 王静, authorCompany=null, correspAuthors=王静, authorNote=, correspAuthorsNote=, copyrightStatement=2025年版权归本刊主办单位所有未经本刊书面许可, 不得转载、摘编本刊所有文章内容。, copyrightOwner=中国科学器材公司, extLink=null, articleAbsUrl=null, sourceXml=2qsCIfmQ1C0NPUzx8zk8kw==, magXml=6XlEp3aLJLnt4Pl1OYqjCQ==, pdfUrl=null, pdf=vvImxOsMxVqHy+WI2vimyw==, pdfFileSize=4842470, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=HKXNyzUM7MKF4xNJAwm5iQ==, mapNumber=null, fund=null)}, authors=[Author(id=1320389091091399319, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389090273510031, orderNo=0, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1320389091166896793, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389090273510031, authorId=1320389091091399319, language=EN, stringName=Wenjuan Bai, firstName=Wenjuan, middleName=null, lastName=Bai, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=null, address=Shanghai Institute of Immunology, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1320389091234005658, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389090273510031, authorId=1320389091091399319, language=CN, stringName=柏文娟, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=null, address=上海交通大学医学院上海市免疫学研究所，上海　200025, bio={"content":"

柏文娟，女，博士研究生，实验师，研究方向：主要从事显微成像平台、大型显微镜的管理工作

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柏文娟，女，博士研究生，实验师，研究方向：主要从事显微成像平台、大型显微镜的管理工作

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