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Article(id=1320389042328420607, tenantId=1146029695717560320, journalId=1319373289681944729, issueId=1320388933154878311, articleNumber=null, orderNo=null, doi=10.11876/mimt202504012, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=null, receivedDateStr=null, revisedDate=null, revisedDateStr=null, acceptedDate=null, acceptedDateStr=null, onlineDate=1792734879332, onlineDateStr=2026-10-23, pubDate=1754755200000, pubDateStr=2025-08-10, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1792734879332, onlineIssueDateStr=2026-10-23, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1792734879332, creator=13701087609, updateTime=1792734879332, updator=13701087609, issue=Issue{id=1320388933154878311, tenantId=1146029695717560320, journalId=1319373289681944729, year='2025', volume='31', issue='4', pageStart='2', pageEnd='132', issueExtLink='null', onlineDate='null', pubDate='1754755200000', pubDateStr='2025-08-10', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1792734853304, creator='13701087609', updateTime=1792746408152, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1320437397758791917, tenantId=1146029695717560320, journalId=1319373289681944729, issueId=1320388933154878311, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1320437397758791918, tenantId=1146029695717560320, journalId=1319373289681944729, issueId=1320388933154878311, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=111, endPage=116, ext={EN=ArticleExt(id=1320389042596856064, articleId=1320389042328420607, tenantId=1146029695717560320, journalId=1319373289681944729, language=EN, title=Applications and prospects of two-photon intravital imaging in the field of life sciences, columnId=null, journalTitle=Modern Instruments & Medical Treatment, columnName=null, runingTitle=null, highlight=null, articleAbstract=

Intravital two-photon imaging technology has the advantage of deep imaging, enabling the direct monitoring of the dynamic processes within living organisms. It has become an important research tool in the field of life sciences. We briefly explain the basic principles of two-photon microscopy and its applications in life science research in recent years. We mainly focuse on analyzing the development trends of intravital two-photon imaging technology and discuss the current challenges and future optimization directions. We look forward to the continuous optimization of intravital two-photon imaging technology, which can promote its wider application.

, authors=Wenjuan Bai, Jin Wang, Wenying Zhao, Jing Wang*, authorsList=Wenjuan Bai, Jin Wang, Wenying Zhao, Jing Wang, authorCompany=null, correspAuthors=Jing Wang, authorNote=null, correspAuthorsNote=null, copyrightStatement=All copyrights in 2025 belong to the sponsor of the journal.Not content published by the journal may be reproduced or abridged without authorization., copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1320389043045646593, articleId=1320389042328420607, tenantId=1146029695717560320, journalId=1319373289681944729, language=CN, title=双光子活体成像在生命科学领域的应用与展望, columnId=1320389006882357264, journalTitle=现代仪器与医疗, columnName=生物应用, runingTitle=null, highlight=null, articleAbstract=

双光子活体成像技术具有深层成像的优势，可以直观地监测活体的体内动态过程，已成为生命科学领域研究的重要手段。本文简要介绍了双光子显微镜的技术原理，综述了双光子显微镜在生命科学研究中的应用领域，重点分析了双光子显微活体成像技术的发展趋势，探讨其当前面临的挑战及未来的优化方向，旨在为进一步优化双光子活体成像技术，推动双光子活体成像技术更加广泛的应用提供参考。

, authors=柏文娟, 王靳, 赵文颖, 王静*, authorsList=柏文娟, 王靳, 赵文颖, 王静, authorCompany=null, correspAuthors=王静, authorNote=

柏文娟，女，博士研究生，实验师，研究方向：主要从事显微成像平台、大型显微镜的管理工作

, correspAuthorsNote=
王静，女，博士研究生，研究员，研究方向：运用活体成像研究天然免疫细胞的动态及功能
, copyrightStatement=2025年版权归本刊主办单位所有未经本刊书面许可, 不得转载、摘编本刊所有文章内容。, copyrightOwner=中国科学器材公司, extLink=null, articleAbsUrl=null, sourceXml=hCgr3JaLbVjK5iIBAqsy2w==, magXml=m3l5urEbD2u88BZfKelx3Q==, pdfUrl=null, pdf=Ry0sEAcBGfW/6lVsjp61aw==, pdfFileSize=1606657, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=noBD0fhHwyebLYLn0CgEQg==, mapNumber=null, fund=null)}, authors=[Author(id=1320389043557351686, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, orderNo=0, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1320389045268627720, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, authorId=1320389043557351686, language=EN, stringName=Wenjuan Bai, firstName=Wenjuan, middleName=null, lastName=Bai, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=null, address=Shanghai Institute of Immunology, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1320389045436399881, tenantId=1146029695717560320, journalId=1319373289681944729, articleId=1320389042328420607, authorId=1320389043557351686, language=CN, stringName=柏文娟, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=null, address=上海交通大学医学院上海市免疫学研究所，上海　200025, bio={"content":"

柏文娟，女，博士研究生，实验师，研究方向：主要从事显微成像平台、大型显微镜的管理工作

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柏文娟，女，博士研究生，实验师，研究方向：主要从事显微成像平台、大型显微镜的管理工作

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双光子活体成像在生命科学领域的应用与展望
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柏文娟 , 王靳 , 赵文颖 , 王静 *
现代仪器与医疗 | 生物应用 2025,31(4): 111-116
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现代仪器与医疗 |生物应用 2025 , 31 (4) : 111 -116
双光子活体成像在生命科学领域的应用与展望
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柏文娟, 王靳, 赵文颖, 王静*
作者信息
  • 上海交通大学医学院上海市免疫学研究所，上海　200025
通讯作者:
王静，女，博士研究生，研究员，研究方向：运用活体成像研究天然免疫细胞的动态及功能
作者简介:

柏文娟，女，博士研究生，实验师，研究方向：主要从事显微成像平台、大型显微镜的管理工作

Applications and prospects of two-photon intravital imaging in the field of life sciences
Wenjuan Bai, Jin Wang, Wenying Zhao, Jing Wang*
Affiliations
  • Shanghai Institute of Immunology, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China
出版时间: 2025-08-10 doi: 10.11876/mimt202504012
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双光子活体成像技术具有深层成像的优势，可以直观地监测活体的体内动态过程，已成为生命科学领域研究的重要手段。本文简要介绍了双光子显微镜的技术原理，综述了双光子显微镜在生命科学研究中的应用领域，重点分析了双光子显微活体成像技术的发展趋势，探讨其当前面临的挑战及未来的优化方向，旨在为进一步优化双光子活体成像技术，推动双光子活体成像技术更加广泛的应用提供参考。

活体成像  /  双光子显微镜  /  生命科学

Intravital two-photon imaging technology has the advantage of deep imaging, enabling the direct monitoring of the dynamic processes within living organisms. It has become an important research tool in the field of life sciences. We briefly explain the basic principles of two-photon microscopy and its applications in life science research in recent years. We mainly focuse on analyzing the development trends of intravital two-photon imaging technology and discuss the current challenges and future optimization directions. We look forward to the continuous optimization of intravital two-photon imaging technology, which can promote its wider application.

Intravital imaging  /  Two-photon microscope  /  Life Sciences
柏文娟, 王靳, 赵文颖, 王静. 双光子活体成像在生命科学领域的应用与展望. 现代仪器与医疗, 2025 , 31 (4) : 111 -116 . DOI: 10.11876/mimt202504012
Wenjuan Bai, Jin Wang, Wenying Zhao, Jing Wang. Applications and prospects of two-photon intravital imaging in the field of life sciences[J]. Modern Instruments & Medical Treatment, 2025 , 31 (4) : 111 -116 . DOI: 10.11876/mimt202504012
基金
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  • 国家自然科学基金面上项目(82371760)
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2025年第31卷第4期
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doi: 10.11876/mimt202504012
  • 首发时间：2026-10-23
  • 出版时间：2025-08-10
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基金
国家自然科学基金面上项目(82371760)
作者信息
    上海交通大学医学院上海市免疫学研究所，上海　200025

通讯作者:

王静，女，博士研究生，研究员，研究方向：运用活体成像研究天然免疫细胞的动态及功能
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