现代仪器与医疗

非干涉定量相位成像与光强衍射层析：理论、方法与仪器

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束业峰 ¹^,² , 周宁 ¹^,² , 孙佳嵩 ¹^,² , 范瑶 ¹^,² , 韦峰 ³^,⁴ , 张晓磊 ³^,⁴ , 左超 ¹^,²^,³^,⁴^,^*

现代仪器与医疗 | 技术进展 2025,31(4): 28-44

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现代仪器与医疗 |技术进展 2025 , 31 (4) : 28 -44

非干涉定量相位成像与光强衍射层析：理论、方法与仪器

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束业峰¹^,², 周宁¹^,², 孙佳嵩¹^,², 范瑶¹^,², 韦峰³^,⁴, 张晓磊³^,⁴, 左超¹^,²^,³^,⁴^,^*

作者信息

^1.南京理工大学电子工程与光电技术学院，智能计算成像实验室，南京　210094

^2.江苏省视觉传感与智能感知重点实验室，南京　210094

^3.南京理工大学智能计算成像研究院，南京　210019

^4.锆石光电（苏州）有限公司，苏州　210026

通讯作者:

左超，男，博士研究生，教授，研究方向：计算光学成像与光信息处理技术

作者简介:

束业峰，男，博士研究生，研究方向：计算光学显微成像

Non-interferometric quantitative phase imaging and intensity diffraction tomography: theory, methods, and instruments

Yefeng Shu¹^,², Ning Zhou¹^,², Jiasong Sun¹^,², Yao Fan¹^,², Feng Wei³^,⁴, Xiaolei Zhang³^,⁴, Chao Zuo¹^,²^,³^,⁴^,^*

Affiliations

^1.Smart Computational Imaging Laboratory, School of Electronic and Optical Engineering, Nanjing University of Science and Technology, Nanjing 210094, China

^2.Jiangsu Key Laboratory of Visual Sensing & Intelligent Perception, Nanjing 210094, China

^3.Smart Computational Imaging Research Institute, Nanjing University of Science and Technology, Nanjing 210019, China

^4.Zircon Optoelectronics (Suzhou) Co., Ltd., Suzhou 210026, China

出版时间: 2025-08-10 doi: 10.11876/mimt202504004

文章导航

摘要

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光学显微镜是推动生命科学持续发展的核心工具。无标记相位成像利用折射率作为内源性对比机制，能够避免荧光标记带来的光毒性与光漂白，正日益成为解析细胞结构与动力学的重要途径。自1932年泽尼克发明相衬显微镜以来，相位成像技术已由二维定性观察迈向三维定量解析，其中数字全息显微和光学衍射层析是两项具有代表性的里程碑方法。然而，这类干涉式技术对高相干光源和复杂干涉光路的依赖，在系统稳定性、成像噪声控制与工程化应用方面存在根本性瓶颈，因而始终未能在生命科学实验室中取代相衬显微镜的普遍地位。近年来，计算光学定量相位成像技术的发展与光强衍射层析技术的兴起，突破了传统干涉测量的固有限制，标志着无标记定量相位成像进入了全新的发展阶段。在此背景下，本团队依托十余年的理论积累与方法创新，将先进的计算成像框架与工程化设计深度融合，率先在国际上研制出新一代基于光强衍射层析的无标记显微成像仪器。该仪器兼具高分辨率、高稳定性与高通量，能够在无标记条件下实现对活细胞结构与动态过程的长期、定量和多尺度观测。本文系统梳理了无标记相位成像的发展脉络，重点总结了本团队在计算光学成像与仪器研发方面的最新进展，并展望其在疾病机理解析、药物筛选、临床诊断以及未来科学仪器体系中的广阔前景。

关键词

无标记成像 / 计算光学显微成像 / 定量相位成像 / 光学衍射层析 / 相位恢复

Abstract

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Optical microscopes are core tools driving the continuous development of life sciences. Label-free phase imaging, which utilizes refractive index as an endogenous contrast mechanism, avoids phototoxicity and photobleaching caused by fluorescent labeling, and is increasingly becoming a crucial approach for analyzing cellular structures and dynamics. Since Zernike invented the phase-contrast microscope in 1932, phase imaging technology has advanced from two-dimensional qualitative observation to three-dimensional quantitative analysis, with digital holographic microscopy and optical diffraction tomography being two representative milestone methods. However, the reliance of such interferometric techniques on highly coherent light sources and complex interference optical paths leads to fundamental bottlenecks in terms of system stability, imaging noise control, and engineering applications. As a result, they have not yet replaced phase-contrast microscopes in terms of widespread use in life science laboratories. In recent years, the development of computational optical quantitative phase imaging technology and the rise of intensity diffraction tomography have broken through the inherent limitations of traditional interferometry, marking the entry of label-free quantitative phase imaging into a new stage of development. Against this backdrop, relying on more than a decade of theoretical accumulation and methodological innovation, our team has deeply integrated advanced computational imaging frameworks with engineering design, and taken the lead in developing a new generation of label-free microscopic imaging instruments based on intensity diffraction tomography internationally. This instrument combines high resolution, high stability, and high throughput, enabling long-term, quantitative, and multi-scale observation of living cell structures and dynamic processes under label-free conditions. This paper systematically reviews the development history of label-free phase imaging, focuses on summarizing the latest progress of our team in computational optical imaging and instrument development, and looks forward to its broad prospects in disease mechanism analysis, drug screening, clinical diagnosis, and the future scientific instrument system.

Key words

Label-free imaging / Computational optical microscopy / Quantitative phase imaging / Optical diffraction tomography / Phase retrieval

引用本文

束业峰, 周宁, 孙佳嵩, 范瑶, 韦峰, 张晓磊, 左超. 非干涉定量相位成像与光强衍射层析：理论、方法与仪器. 现代仪器与医疗, 2025 , 31 (4) : 28 -44 . DOI: 10.11876/mimt202504004

Yefeng Shu, Ning Zhou, Jiasong Sun, Yao Fan, Feng Wei, Xiaolei Zhang, Chao Zuo. Non-interferometric quantitative phase imaging and intensity diffraction tomography: theory, methods, and instruments[J]. Modern Instruments & Medical Treatment, 2025 , 31 (4) : 28 -44 . DOI: 10.11876/mimt202504004

基金

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国家自然科学基金(62227818; 62361136588; 62305162; U21B2033)
国家重点研发计划(2022YFA1205002; 2024YFE0101300; 2024YFF0505603; 2024YFF0505600)
江苏省科技计划生物医药竞争类项目(BE2022847)
江苏省科技计划重点国别产业技术研发合作项目(BZ2022039)
中央高校科研专项资助项目(30923010206)
中央高校基础科研业务费专项资金资助项目(2023102001; 2024202002)
等离子体物理全国重点实验室项目(JCKYS2024212804)
江苏省光谱成像与智能感知重点实验室开放基金(JSGP202105; JSGP202201; JSGPCXZNGZ202401)

参考文献

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2025年第31卷第4期

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doi: 10.11876/mimt202504004

首发时间：2026-10-23
出版时间：2025-08-10

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基金

国家自然科学基金(62227818; 62361136588; 62305162; U21B2033)

国家重点研发计划(2022YFA1205002; 2024YFE0101300; 2024YFF0505603; 2024YFF0505600)

江苏省科技计划生物医药竞争类项目(BE2022847)

江苏省科技计划重点国别产业技术研发合作项目(BZ2022039)

中央高校科研专项资助项目(30923010206)

中央高校基础科研业务费专项资金资助项目(2023102001; 2024202002)

等离子体物理全国重点实验室项目(JCKYS2024212804)

江苏省光谱成像与智能感知重点实验室开放基金(JSGP202105; JSGP202201; JSGPCXZNGZ202401)

作者信息

^1.南京理工大学电子工程与光电技术学院，智能计算成像实验室，南京　210094

^2.江苏省视觉传感与智能感知重点实验室，南京　210094

^3.南京理工大学智能计算成像研究院，南京　210019

^4.锆石光电（苏州）有限公司，苏州　210026

通讯作者:

左超，男，博士研究生，教授，研究方向：计算光学成像与光信息处理技术

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2种不同金属材料的力学参数

科 Family	属数 Number of genus	种数 Number of species	占总种数比例 Percentage of total species (%)	属 Genus	种数 Number of species	占总种数比例 Percentage of total species (%)
鹅膏菌科Amanitaceae	2	11	5.26	鹅膏菌属 Amanita	10	4.78
小菇科 Mycenaceae	2	12	5.74	丝盖伞属 Inocybe	5	2.39
多孔菌科 Polyporaceae	8	14	6.70	蜡蘑属 Laccaria	5	2.39
红菇科 Russulaceae	3	23	11.00	小皮伞属 Marasmius	6	2.87
				小菇属 Mycena	11	5.26
				光柄菇属 Pluteus	5	2.39
				红菇属 Russula	17	8.13
				栓菌属 Trametes	5	2.39

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