中国组织工程研究

牙髓干细胞及衍生产物在牙髓再生中的应用与进展

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徐海超 ¹, , 罗丽花 ¹, , 潘乙怀 ¹,

中国组织工程研究 | 2026,30(1): 153-162

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中国组织工程研究 2026 , 30 (1) : 153 -162

牙髓干细胞及衍生产物在牙髓再生中的应用与进展

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徐海超¹,, 罗丽花¹,, 潘乙怀¹,

作者信息

^1.州医科大学口腔医学院附属口腔医院，浙江省温州市 325000

^2.州医科大学口腔医学院附属口腔医院，浙江省温州市 325000

Application and progress of dental pulp stem cells and their derivatives in dental pulp regeneration

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出版时间: 2026-01-08 doi: 10.12307/2025.572

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摘要

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背景：牙髓干细胞是一类源自牙髓组织的牙源性间充质干细胞，具有良好的自我更新和多向分化潜力。近年来，牙髓干细胞及其衍生产物包括细胞外囊泡、条件培养液以及脱细胞基质等，在牙髓组织损伤修复再生中的应用研究取得了显著进展，显示出广阔的临床应用前景。目的：系统综述牙髓干细胞及其衍生产物在牙髓组织工程中的研究成果和应用进展。方法：检索PubMed数据库、中国生物医学文献数据库、中国知网，以“牙髓干细胞，细胞外囊泡，外泌体，凋亡小体，条件培养液，脱细胞基质，再生”为中文检索词，以“dental pulp stem cells，extracellular vesicles，exosomes，apoptotic bodies，conditioned medium，decellularized matrix，regeneration”为英文检索词进行检索，检索时限为2005年1月至2023年6月。根据文题和摘要对初检文献进行筛选，排除重复文献和与主题不相关的文献，最终纳入103篇与牙髓再生高度相关的文献进行综述分析。结果与结论：牙髓干细胞及其衍生产物富含多种生物活性因子，能够有效促进成牙本质、成血管和成神经分化，在牙髓-牙本质复合体的形成过程中展现出巨大的应用潜力。然而，牙髓干细胞及其衍生产物在临床应用转化中仍面临挑战，未来的研究应着重于优化制备流程、明确作用机制和完善安全性评价，为推动临床牙髓损伤修复提供新的治疗策略。

关键词

牙髓干细胞 / 细胞外囊泡 / 条件培养液 / 脱细胞基质 / 牙髓再生 / 组织工程 / 工程化干细胞

Abstract

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BACKGROUND: Dental pulp stem cells, which derived from the dental pulp tissue, are one type of dental-derived mesenchymal stem cells, possess significant properties of self-renewal and multi-lineage differentiation. In recent years, series of researches focus on dental pulp stem cells and their derivatives such as extracellular vesicles, conditioned medium, and decellularized extracellular matrix, those have positive effects on the repair and regeneration of pulp tissue injury, showing an attractive clinical application. OBJECTIVE: To systematically review the researches and applications of dental pulp stem cells and their derivatives in dental pulp tissue engineering. METHODS: Literature searches were conducted in PubMed, China Biology Medicine (CBM), and China National Knowledge Infrastructure (CNKI) databases for articles published from January 2005 to June 2023. The search terms included “dental pulp stem cells, extracellular vesicles, exosomes, apoptotic bodies, conditioned medium, decellularized matrix, regeneration” in Chinese and English. After screening the titles and abstracts, duplicate and irrelevant studies were excluded. Finally, 103 studies closely related to dental pulp regeneration were included for review and analysis. RESULTS AND CONCLUSION: Dental pulp stem cells and their derivatives are rich in lots of bioactive factors that can effectively promote odontogenic, angiogenic, and neurogenic differentiation, exhibiting significant potential in the formation of the pulp-dentin complex. However, the clinical translation of dental pulp stem cells and their derivatives still faces several challenges. Future researches should focus on optimizing preparation protocols, elucidating the underlying mechanisms of action, and refining safety assessments to provide novel therapeutic strategies for the repair of dental pulp injury.

Key words

dental pulp stem cell / extracellular vesicle / conditioned medium / decellularized matrix / dental pulp regeneration / tissue engineering / engineered stem cell

引用本文

徐海超, 罗丽花, 潘乙怀. 牙髓干细胞及衍生产物在牙髓再生中的应用与进展. 中国组织工程研究, 2026 , 30 (1) : 153 -162 . DOI: 10.12307/2025.572

. Application and progress of dental pulp stem cells and their derivatives in dental pulp regeneration[J]. Chinese Journal of Tissue Engineering Research, 2026 , 30 (1) : 153 -162 . DOI: 10.12307/2025.572

基金

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浙江省基础公益研究计划项目

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2026年第30卷第1期

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doi: 10.12307/2025.572

首发时间：2025-05-30
出版时间：2026-01-08

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录用日期：2025-02-11

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浙江省基础公益研究计划项目

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^1.州医科大学口腔医学院附属口腔医院，浙江省温州市 325000

^2.州医科大学口腔医学院附属口腔医院，浙江省温州市 325000

通讯作者:

罗丽花: 潘乙怀，博士，教授，博士研究生导师，温州医科大学口腔医学院附属口腔医院，浙江省温州市 325000

通讯作者:

潘乙怀: 罗丽花，博士，副研究员，硕士研究生导师，温州医科大学口腔医学院附属口腔医院，浙江省温州市 325000

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2种不同金属材料的力学参数

科 Family	属数 Number of genus	种数 Number of species	占总种数比例 Percentage of total species (%)	属 Genus	种数 Number of species	占总种数比例 Percentage of total species (%)
鹅膏菌科Amanitaceae	2	11	5.26	鹅膏菌属 Amanita	10	4.78
小菇科 Mycenaceae	2	12	5.74	丝盖伞属 Inocybe	5	2.39
多孔菌科 Polyporaceae	8	14	6.70	蜡蘑属 Laccaria	5	2.39
红菇科 Russulaceae	3	23	11.00	小皮伞属 Marasmius	6	2.87
				小菇属 Mycena	11	5.26
				光柄菇属 Pluteus	5	2.39
				红菇属 Russula	17	8.13
				栓菌属 Trametes	5	2.39

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