中国组织工程研究

人脐血富血小板血浆、单个核细胞及间充质干细胞修复大鼠薄型子宫内膜

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牟彦郦 ¹ , 胡安春 ¹ , 须文驰 ¹ , 陈盼盼 ¹ , 陈浩 ², , 赵淑云 ², , 黄官友 ², , 陈小娟 ⁴

中国组织工程研究 | 工程化脐带脐血干细胞 2026,30(1): 78-92

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中国组织工程研究 |工程化脐带脐血干细胞 2026 , 30 (1) : 78 -92

人脐血富血小板血浆、单个核细胞及间充质干细胞修复大鼠薄型子宫内膜

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牟彦郦¹, 胡安春¹, 须文驰¹, 陈盼盼¹, 陈浩²,, 赵淑云²,, 黄官友²,, 陈小娟⁴

作者信息

^1.州医科大学，贵州省贵阳市 550004

^2.州医科大学附属医院生殖中心，贵州省贵阳市 550004

^3.州医科大学附属医院生殖中心，贵州省贵阳市 550004

^4.州医科大学附属医院高压氧科，贵州省贵阳市 550004

Effects of human umbilical cord blood platelet-rich plasma, mononuclear cells, and mesenchymal stem cells in repairing thin endometrium in rats

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出版时间: 2026-01-08 doi: 10.12307/2026.510

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摘要

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背景：研究发现人脐血富血小板血浆与人脐血间充质干细胞对薄型子宫内膜有一定治疗效果，但目前针对人脐血单个核细胞对薄型子宫内膜的研究尚未报道，且目前尚缺乏三者比较的相关研究。目的：探讨人脐血富血小板血浆、单个核细胞及间充质干细胞修复大鼠薄型子宫内膜的效果与机制。方法：60只雌性SPF级SD大鼠随机分为假手术组、模型组、人脐血富血小板血浆组、人脐血单个核细胞组和人脐血间充质干细胞组，每组12只。假手术组宫角注射0.5 mL生理盐水，5 min后再灌注0.5 mL PBS；模型组、人脐血富血小板血浆组、人脐血单个核细胞组和人脐血间充质干细胞组宫角注射0.5 mL体积分数95%乙醇，5 min后吸出剩余乙醇，经生理盐水冲洗2次后再分别灌注0.5 mL PBS、人脐血富血小板血浆、人脐血单个核细胞悬液(1×107个)和人脐血间充质干细胞悬液(1×107个)，于再灌注后第3个正常动情周期的动情间期收集脏器组织与血清，检测相关指标。结果与结论：①子宫组织大体观、苏木精-伊红染色和Masson染色结果：假手术组结构完整，内膜厚度适中，血管结构清晰。与假手术组相比，模型组子宫萎缩，结构不完整，内膜厚度、腺体数量明显减少，血管结构紊乱，纤维化增加；与模型组相比，经人脐血衍生物治疗后，子宫大小、结构、内膜厚度均有所恢复(均P < 0.01)，并且纤维化均减轻，其中以人脐血单个核细胞组恢复最为明显。在腺体数量增加方面，以人脐血富血小板血浆组效果最为显著(P < 0.000 1)。②子宫组织免疫组化与免疫荧光结果显示，与假手术组相比，模型组细胞增殖相关指标角蛋白9、波形蛋白，内膜容受性相关指标白血病抑制因子、整合素αγβ3，血小板内皮细胞黏附分子、碱性成纤维细胞生长因子、血管内皮生长因子表达均降低(均P < 0.05)。与模型组相比，经人脐血衍生物治疗后以上指标均有显著性升高，人脐血衍生物各组比较：角蛋白9、血管内皮生长因子蛋白：人脐血单个核细胞组>人脐血间充质干细胞组>人脐血富血小板血浆组；波形蛋白、白血病抑制因子蛋白：人脐血间充质干细胞组>人脐血单个核细胞组>人脐血富血小板血浆组；整合素αγβ3蛋白：人脐血富血小板血浆组>人脐血间充质干细胞组>人脐血单个核细胞组；血小板内皮细胞黏附分子蛋白：人脐血富血小板血浆组>人脐血单个核细胞组>人脐血间充质干细胞组；碱性成纤维细胞生长因子蛋白：人脐血单个核细胞组>人脐血富血小板血浆组>人脐血间充质干细胞组。③Western blot检测显示，与假手术组相比，模型组白细胞介素6、白细胞介素1β和肿瘤坏死因子α蛋白表达水平显著升高(均P < 0.001)，经人脐血衍生物治疗后上述指标均降低(均P < 0.05)。④ELISA检测显示，与假手术组相比，模型组抗缪勒氏管激素、雌二醇、孕酮水平降低，促卵泡生成素、促黄体生成素水平增加(除促黄体生成素外，均P < 0.05)。经人脐血衍生物治疗后，性激素和抗缪勒氏管激素水平有一定程度恢复。⑤生育力实验显示，与假手术组相比，模型组受孕时间有所增加，产仔数明显降低(均P < 0.05)。经人脐血衍生物治疗后，3组产仔数均有所增加(P < 0.05)，组间比较尚未发现明显差异。此研究初步表明人脐血单个核细胞对薄型子宫内膜有一定治疗效果，且人脐血富血小板血浆、人脐血单个核细胞和人脐血间充质干细胞分别在改善薄型子宫内膜再生功能、内膜容受性、血管生成、炎症调控及妊娠结局方面具有不同的优势与差异。

关键词

人脐血富血小板血浆 / 人脐血单个核细胞 / 人脐血间充质干细胞 / 薄型子宫内膜 / SD大鼠

Abstract

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BACKGROUND: Research has found that human umbilical cord blood platelet rich plasma and human umbilical cord blood derived mesenchymal stem cells have certain therapeutic effects on thin endometrium. However, there are currently no reports on the study of human umbilical cord blood mononuclear cells on thin endometrium, and there is a lack of relevant research comparing the three. OBJECTIVE: To explore the effects and mechanisms of human umbilical cord blood platelet rich plasma, monocytes, and mesenchymal stem cells in repairing thin endometrium in rats. METHODS: Sixty female SPF grade SD rats were randomly divided into sham operation group, model group, human umbilical cord blood platelet rich plasma group, human umbilical cord blood mononuclear cell group, and human umbilical cord blood derived mesenchymal stem cell group, with 12 rats in each group. The sham operation group received 0.5 mL physiological saline injection into the uterine horn, followed by 0.5 mL of PBS infusion after 5 minutes; The model group, human umbilical cord blood platelet rich plasma group, human umbilical cord blood mononuclear cell group, and human umbilical cord blood derived mesenchymal stem cell group were injected with 0.5 mL of 95% ethanol by volume. After 5 minutes, the remaining ethanol was aspirated and washed twice with physiological saline. Then, 0.5 mL of PBS, human umbilical cord blood platelet rich plasma, human umbilical cord blood mononuclear cells (1×107 cells/mL), and human umbilical cord blood derived mesenchymal stem cells (1×107 cells/mL) were perfused separately. During the third normal estrus cycle after reperfusion, organs, tissues and serum were collected for testing of relevant indicators. RESULTS AND CONCLUSION: (1) The macroscopic view of uterine tissue, hematoxylin eosin staining and Masson staining results: the sham operation group had intact structure, moderate endometrial thickness, and clear vascular structure. Compared with the sham operation group, the model group showed uterine atrophy, incomplete structure, significantly reduced endometrial thickness and glandular quantity, disordered vascular structure, and increased fibrosis. Compared with the model group, after treatment with human umbilical cord blood derivatives, the size, structure, and endometrial thickness of the uterus were restored (all P < 0.01), and fibrosis was reduced, with the most significant recovery observed in the human umbilical cord blood mononuclear cell group. The increase in glandular quantity was most significant in the human umbilical cord blood platelet rich plasma group (P < 0.000 1). (2) The immunohistochemistry and immunofluorescence results of uterine tissue showed that compared with the sham operation group, the expression levels of cell proliferation related indicators such as keratin 9 and vimentin, endometrial receptivity related indicators such as leukemia inhibitory factor and integrin αγβ3, platelet endothelial cell adhesion molecule, basic fibroblast growth factor, and vascular endothelial growth factor were all reduced in the model group (all P < 0.05). Compared with the model group, the above indicators were significantly increased after treatment with human umbilical cord blood derivatives. Comparison of human umbilical cord blood derivatives among groups showed that keratin 9 and vascular endothelial growth factor protein: human umbilical cord blood mononuclear cell group > human umbilical cord blood derived mesenchymal stem cell group > human umbilical cord blood platelet rich plasma group; Wave shaped protein and leukemia inhibitory factor protein: human umbilical cord blood derived mesenchymal stem cell group > human umbilical cord blood mononuclear cell group > human umbilical cord blood platelet rich plasma group; Integrin αγβ3 protein: human umbilical cord blood platelet rich plasma group > human umbilical cord blood derived mesenchymal stem cell group > human umbilical cord blood mononuclear cell group; Platelet endothelial cell adhesion molecule protein: human umbilical cord blood platelet rich plasma group > human umbilical cord blood mononuclear cell group > human umbilical cord blood derived mesenchymal stem cell group; Basic fibroblast growth factor protein: human umbilical cord blood mononuclear cell group > human umbilical cord blood platelet rich plasma group > human umbilical cord blood derived mesenchymal stem cell group. (3) Western blot analysis showed that compared with the sham operation group, the protein levels of interleukin-6, interleukin-1β, and tumor necrosis factor alpha in the model group were significantly increased (all P < 0.001), and their expression levels decreased after treatment (all P < 0.05). (4) ELISA assay showed that compared with the sham operation group, the model group had lower levels of anti Mullerian hormone, estradiol, and progesterone, and increased levels of follicle stimulating hormone and luteinizing hormone (except for luteinizing hormone, all P < 0.05). After treatment, there was a certain degree of recovery in the levels of sex hormones and anti Mullerian hormones. (5) Fertility experiments showed that compared with the sham operation group, the model group had an increase in conception time and a significant decrease in litter size (all P < 0.05). After treatment with human umbilical cord blood derivatives, the litter size of all three groups increased (P < 0.05), and no significant differences were found between the groups. This study preliminarily reveals that human umbilical cord blood mononuclear cells have a certain therapeutic effect on thin endometrium, and human umbilical cord blood platelet rich plasma, human umbilical cord blood mononuclear cells, and human umbilical cord blood derived mesenchymal stem cells have different advantages and differences in improving endometrial regeneration function, endometrial receptivity, angiogenesis, inflammation regulation, and improving pregnancy outcomes in thin endometrium.

Key words

human umbilical cord blood platelet-rich plasma / human umbilical cord blood mononuclear cell / human umbilical cord blood derived mesenchymal stem cell / thin endometrium / SD rat

引用本文

牟彦郦, 胡安春, 须文驰, 陈盼盼, 陈浩, 赵淑云, 黄官友, 陈小娟. 人脐血富血小板血浆、单个核细胞及间充质干细胞修复大鼠薄型子宫内膜. 中国组织工程研究, 2026 , 30 (1) : 78 -92 . DOI: 10.12307/2026.510

. Effects of human umbilical cord blood platelet-rich plasma, mononuclear cells, and mesenchymal stem cells in repairing thin endometrium in rats[J]. Chinese Journal of Tissue Engineering Research, 2026 , 30 (1) : 78 -92 . DOI: 10.12307/2026.510

基金

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贵州省卫生健康委科学技术基金项目
贵州省卫生健康委科学技术基金项目
贵州省卫生健康委科学技术基金项目

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2026年第30卷第1期

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doi: 10.12307/2026.510

首发时间：2025-05-30
出版时间：2026-01-08

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录用日期：2025-02-28

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贵州省卫生健康委科学技术基金项目

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^1.州医科大学，贵州省贵阳市 550004

^2.州医科大学附属医院生殖中心，贵州省贵阳市 550004

^3.州医科大学附属医院生殖中心，贵州省贵阳市 550004

^4.州医科大学附属医院高压氧科，贵州省贵阳市 550004

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2种不同金属材料的力学参数

科 Family	属数 Number of genus	种数 Number of species	占总种数比例 Percentage of total species (%)	属 Genus	种数 Number of species	占总种数比例 Percentage of total species (%)
鹅膏菌科Amanitaceae	2	11	5.26	鹅膏菌属 Amanita	10	4.78
小菇科 Mycenaceae	2	12	5.74	丝盖伞属 Inocybe	5	2.39
多孔菌科 Polyporaceae	8	14	6.70	蜡蘑属 Laccaria	5	2.39
红菇科 Russulaceae	3	23	11.00	小皮伞属 Marasmius	6	2.87
				小菇属 Mycena	11	5.26
				光柄菇属 Pluteus	5	2.39
				红菇属 Russula	17	8.13
				栓菌属 Trametes	5	2.39

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