首页 期刊 论文 学科刊群 资讯 加盟 关于
EN
image
Article(id=1248601952666412025, tenantId=1146029695717560320, journalId=1190317699101192196, issueId=1248601950581842932, articleNumber=1001-2494(2024)08-0687-07, orderNo=null, doi=null, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=research-article, receivedDate=1676908800000, receivedDateStr=2023-02-21, revisedDate=null, revisedDateStr=null, acceptedDate=null, acceptedDateStr=null, onlineDate=1775619503417, onlineDateStr=2026-04-08, pubDate=1713715200000, pubDateStr=2024-04-22, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1775619503417, onlineIssueDateStr=2026-04-08, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1775619503417, creator=13701087609, updateTime=1775619503417, updator=13701087609, issue=Issue{id=1248601950581842932, tenantId=1146029695717560320, journalId=1190317699101192196, year='2024', volume='59', issue='8', pageStart='657', pageEnd='754', issueExtLink='null', onlineDate='null', pubDate='null', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1775619502920, creator=13701087609, updateTime=1775620003727, updator=13701087609, preIssue=null, nextIssue=null, ext={EN=IssueExt(id=1248604051202527794, tenantId=1146029695717560320, journalId=1190317699101192196, issueId=1248601950581842932, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1248604051202527795, tenantId=1146029695717560320, journalId=1190317699101192196, issueId=1248601950581842932, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null}, startPage=687, endPage=693, ext={EN=ArticleExt(id=1248601952897098747, articleId=1248601952666412025, tenantId=1146029695717560320, journalId=1190317699101192196, language=EN, title=Identification of Tibetan Medicine Jiawa and Easily Mixed Species Based on ITS2 Barcode, columnId=null, journalTitle=Chinese Pharmaceutical Journal, columnName=null, runingTitle=null, highlight=null, articleAbstract=

OBJECTIVE To establish an effective identification method for Jiawa and easily mixed species by using DNA barcoding ITS2 sequence. METHODS The total DNA of 72 Jiawa and its similar species samples were extracted by using the plant genomic DNA Extraction kit,PCR amplification and bidirectional sequencing were carried out for the ITS2 fragments, another 30 items of the genus were downloaded from GenBank. MEGA6.0 software was applied to analyze 102 ITS2 sequences, calculate the intraspecific and interspecific K2-P distance, construct neighber-jioning (N-J) phylogenetic tree, and predict the secondary structure of ITS2. RESULTS K2-P genetic distance analysis showed that, in addition to Pleurospermum franchetianum Hemsl. and Pleurospermum wrightianum de Boiss., Pleurospermum hookeri var. thomsonii C.B. Clarke., Sphallerocarpus gracilis (Bess.) K.-Pol., Vicatia thibetica de Boiss., Carum carvi L., Heracleum millefolium Diels., Ligusticum daucoides (Franch.) Franch. and Anthriscus sylvestris subsp. Nemorosa have obvious barcode spacing. N-J tree cluster analysis showed that each species could be clustered into one branch with a bootstrap support rate of >50%, and the monophyleticity was good. The secondary structure analysis of ITS2 showed that the number, size and position of stem-loops in the four helical regions of Jiawa and its miscible were different. CONCLUSION The ITS2 sequence can be used to identify Jiawa and its easily mixed species, which is beneficial to the rational development and utilization of Jiawa resources.

, correspAuthors=Bixing GAO, Yan LIAN, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, authorCompany=null, fund=null, authors=null, authorsList=Jiyu YANG, Bixing GAO, Jingliang QI, Yiyu WANG, Qian LI, Yan LIAN), CN=ArticleExt(id=1248601953996005406, articleId=1248601952666412025, tenantId=1146029695717560320, journalId=1190317699101192196, language=CN, title=藏药加哇及其易混品的ITS2序列鉴定研究, columnId=1190352405612040510, journalTitle=中国药学杂志, columnName=论著, runingTitle=null, highlight=null, articleAbstract=

目的 利用DNA条形码核基因内部转录间隔区(ITS2)序列,建立加哇及其易混品的有效鉴定方法。方法 采用试剂盒提取72批加哇及其易混品的DNA,针对其ITS2片段进行聚合酶链式反应(PCR)扩增并双向测序,获取ITS2序列信息,同时从GenBank下载相关序列30条,应用MEGA6.0软件对102条ITS2序列进行序列比对分析,计算种内和种间Kimura双参数遗传距离(K2-P),构建邻接法(neighbor-joining,N-J)系统进化树,并预测ITS2二级结构。结果 K2-P遗传距离分析显示,除松潘棱子芹与瘤果棱子芹外,西藏棱子芹、迷果芹、西藏凹乳芹、葛缕子、裂叶独活、羽苞藁本及刺果峨参具有明显的条形码间距。N-J树聚类分析显示,各物种均能以>50%的自展支持率各自聚为1支,单系性良好。ITS2二级结构分析显示,加哇与其易混品在4个螺旋区的茎环数目、大小、位置有所差异。结论 ITS2序列可用来鉴别加哇及其易混品,有利于加哇的质量控制及资源利用。

, correspAuthors=高必兴, 连艳, authorNote=null, correspAuthorsNote=
*高必兴,男,主管中药师 研究方向:中药及民族药质量研究 Tel:(028)87877143;
连艳,女,高级实验师研究方向:中药品种、品质与资源研究 Tel:(028)61800231
, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=La4Cx/NjX8/k9VyNIVLpHQ==, magXml=4MvcLa8aCCMsaNoNAJIi+A==, pdfUrl=null, pdf=7nTK/uUhi8TwPaGkh4yI9A==, pdfFileSize=1322241, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=mEy49/JL5FnZGKj+lYlbAg==, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=MitFog+FPltRjsqQs4xcLg==, mapNumber=null, authorCompany=null, fund=null, authors=

杨吉玉,女,助教 研究方向:中药品种、质量与资源开发应用研究

, authorsList=杨吉玉, 高必兴, 齐景梁, 王艺娱, 李倩, 连艳)}, authors=[Author(id=1249073235380343216, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601952666412025, orderNo=0, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1249073235548115379, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601952666412025, authorId=1249073235380343216, language=EN, stringName=Jiyu YANG, firstName=Jiyu, middleName=null, lastName=YANG, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, 2, address=1 Key Laboratory of Quality Evaluation of Chinese Patent Medicines, Sichuan Institute for Drug Control, NMPA, Chengdu 611731, China
2 Luzhou College of Medical Devices, Luzhou 646000, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1249073235602641332, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601952666412025, authorId=1249073235380343216, language=CN, stringName=杨吉玉, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, 2, address=1 四川省药品检验研究院, 国家药品监督管理局中成药质量评价重点实验室, 成都 611731
2 泸州医疗器械职业学院, 四川 泸州 646000, bio={"content":"

杨吉玉,女,助教 研究方向:中药品种、质量与资源开发应用研究

"}, bioImg=null, bioContent=

杨吉玉,女,助教 研究方向:中药品种、质量与资源开发应用研究

, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1249073235061576102, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601952666412025, xref=1, ext=[AuthorCompanyExt(id=1249073235069964711, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601952666412025, companyId=1249073235061576102, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1 Key Laboratory of Quality Evaluation of Chinese Patent Medicines, Sichuan Institute for Drug Control, NMPA, Chengdu 611731, China), AuthorCompanyExt(id=1249073235078353320, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601952666412025, companyId=1249073235061576102, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1 四川省药品检验研究院, 国家药品监督管理局中成药质量评价重点实验室, 成都 611731)]), AuthorCompany(id=1249073235162239401, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601952666412025, xref=2, ext=[AuthorCompanyExt(id=1249073235170628010, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601952666412025, companyId=1249073235162239401, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2 Luzhou College of Medical Devices, Luzhou 646000, China), AuthorCompanyExt(id=1249073235183210923, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601952666412025, companyId=1249073235162239401, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2 泸州医疗器械职业学院, 四川 泸州 646000)])]), Author(id=1249073235657167288, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601952666412025, orderNo=1, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=1, authorType=1, ext={EN=AuthorExt(id=1249073237267780031, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601952666412025, authorId=1249073235657167288, language=EN, stringName=Bixing GAO, firstName=Bixing, middleName=null, lastName=GAO, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, *, address=1 Key Laboratory of Quality Evaluation of Chinese Patent Medicines, Sichuan Institute for Drug Control, NMPA, Chengdu 611731, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1249073237401997767, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601952666412025, authorId=1249073235657167288, language=CN, stringName=高必兴, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, *, address=1 四川省药品检验研究院, 国家药品监督管理局中成药质量评价重点实验室, 成都 611731, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1249073235061576102, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601952666412025, xref=1, ext=[AuthorCompanyExt(id=1249073235069964711, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601952666412025, companyId=1249073235061576102, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1 Key Laboratory of Quality Evaluation of Chinese Patent Medicines, Sichuan Institute for Drug Control, NMPA, Chengdu 611731, China), AuthorCompanyExt(id=1249073235078353320, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601952666412025, companyId=1249073235061576102, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1 四川省药品检验研究院, 国家药品监督管理局中成药质量评价重点实验室, 成都 611731)])]), Author(id=1249073237481689548, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601952666412025, orderNo=2, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1249073237578158548, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601952666412025, authorId=1249073237481689548, language=EN, stringName=Jingliang QI, firstName=Jingliang, middleName=null, lastName=QI, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, address=1 Key Laboratory of Quality Evaluation of Chinese Patent Medicines, Sichuan Institute for Drug Control, NMPA, Chengdu 611731, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1249073237657850328, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601952666412025, authorId=1249073237481689548, language=CN, stringName=齐景梁, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, address=1 四川省药品检验研究院, 国家药品监督管理局中成药质量评价重点实验室, 成都 611731, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1249073235061576102, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601952666412025, xref=1, ext=[AuthorCompanyExt(id=1249073235069964711, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601952666412025, companyId=1249073235061576102, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1 Key Laboratory of Quality Evaluation of Chinese Patent Medicines, Sichuan Institute for Drug Control, NMPA, Chengdu 611731, China), AuthorCompanyExt(id=1249073235078353320, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601952666412025, companyId=1249073235061576102, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1 四川省药品检验研究院, 国家药品监督管理局中成药质量评价重点实验室, 成都 611731)])]), Author(id=1249073237733347804, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601952666412025, orderNo=3, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1249073237817233889, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601952666412025, authorId=1249073237733347804, language=EN, stringName=Yiyu WANG, firstName=Yiyu, middleName=null, lastName=WANG, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=3, address=3 College of Pharmacy, Chengdu University of Traditional Chinese Medicine, State Key Laboratory of Traditional Chinese Medicine Resources of Southwestern China, Key Laboratory of Standardization of Chinese Herbal Medicine, Chengdu 611137, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1249073237922091495, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601952666412025, authorId=1249073237733347804, language=CN, stringName=王艺娱, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=3, address=3 成都中医药大学药学院, 西南特色中药资源国家重点实验室,中药材标准化教育部重点实验室, 四川 成都 611137, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1249073235246125484, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601952666412025, xref=3, ext=[AuthorCompanyExt(id=1249073235254514093, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601952666412025, companyId=1249073235246125484, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=3 College of Pharmacy, Chengdu University of Traditional Chinese Medicine, State Key Laboratory of Traditional Chinese Medicine Resources of Southwestern China, Key Laboratory of Standardization of Chinese Herbal Medicine, Chengdu 611137, China), AuthorCompanyExt(id=1249073235262902702, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601952666412025, companyId=1249073235246125484, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=3 成都中医药大学药学院, 西南特色中药资源国家重点实验室,中药材标准化教育部重点实验室, 四川 成都 611137)])]), Author(id=1249073238094057969, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601952666412025, orderNo=4, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1249073238198915572, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601952666412025, authorId=1249073238094057969, language=EN, stringName=Qian LI, firstName=Qian, middleName=null, lastName=LI, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, address=1 Key Laboratory of Quality Evaluation of Chinese Patent Medicines, Sichuan Institute for Drug Control, NMPA, Chengdu 611731, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1249073238261830134, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601952666412025, authorId=1249073238094057969, language=CN, stringName=李倩, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, address=1 四川省药品检验研究院, 国家药品监督管理局中成药质量评价重点实验室, 成都 611731, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1249073235061576102, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601952666412025, xref=1, ext=[AuthorCompanyExt(id=1249073235069964711, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601952666412025, companyId=1249073235061576102, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1 Key Laboratory of Quality Evaluation of Chinese Patent Medicines, Sichuan Institute for Drug Control, NMPA, Chengdu 611731, China), AuthorCompanyExt(id=1249073235078353320, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601952666412025, companyId=1249073235061576102, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1 四川省药品检验研究院, 国家药品监督管理局中成药质量评价重点实验室, 成都 611731)])]), Author(id=1249073238391853564, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601952666412025, orderNo=5, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=1, authorType=1, ext={EN=AuthorExt(id=1249073238467351041, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601952666412025, authorId=1249073238391853564, language=EN, stringName=Yan LIAN, firstName=Yan, middleName=null, lastName=LIAN, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=3, *, address=3 College of Pharmacy, Chengdu University of Traditional Chinese Medicine, State Key Laboratory of Traditional Chinese Medicine Resources of Southwestern China, Key Laboratory of Standardization of Chinese Herbal Medicine, Chengdu 611137, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1249073238601568772, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601952666412025, authorId=1249073238391853564, language=CN, stringName=连艳, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=3, *, address=3 成都中医药大学药学院, 西南特色中药资源国家重点实验室,中药材标准化教育部重点实验室, 四川 成都 611137, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1249073235246125484, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601952666412025, xref=3, ext=[AuthorCompanyExt(id=1249073235254514093, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601952666412025, companyId=1249073235246125484, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=3 College of Pharmacy, Chengdu University of Traditional Chinese Medicine, State Key Laboratory of Traditional Chinese Medicine Resources of Southwestern China, Key Laboratory of Standardization of Chinese Herbal Medicine, Chengdu 611137, China), AuthorCompanyExt(id=1249073235262902702, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601952666412025, companyId=1249073235246125484, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=3 成都中医药大学药学院, 西南特色中药资源国家重点实验室,中药材标准化教育部重点实验室, 四川 成都 611137)])])], keywords=[Keyword(id=1249073238777729546, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601952666412025, language=EN, orderNo=1, keyword=Jiawa), Keyword(id=1249073238878392847, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601952666412025, language=EN, orderNo=2, keyword=ITS2 barcode), Keyword(id=1249073238953890321, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601952666412025, language=EN, orderNo=3, keyword=mixed species), Keyword(id=1249073239033582101, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601952666412025, language=EN, orderNo=4, keyword=Tibetan medicine), Keyword(id=1249073239125856795, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601952666412025, language=CN, orderNo=1, keyword=加哇), Keyword(id=1249073239230714399, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601952666412025, language=CN, orderNo=2, keyword=核基因内部转录间隔区序列), Keyword(id=1249073239394292259, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601952666412025, language=CN, orderNo=3, keyword=易混品), Keyword(id=1249073239465595429, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601952666412025, language=CN, orderNo=4, keyword=藏药)], refs=[Reference(id=1249073243345326729, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601952666412025, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[1], rfOrder=0, authorNames=Yutuo·Yuandangongbu, journalName=Four Medical Classics(四部医典), refType=null, unstructuredReference=Yutuo·Yuandangongbu. Four Medical Classics(四部医典)[M]. Shanghai: Shanghai Scientific & Technical Publishers,1987:169., articleTitle=null, refAbstract=null), Reference(id=1249073243462767244, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601952666412025, doi=null, pmid=null, pmcid=null, year=1995, volume=null, issue=null, pageStart=340, pageEnd=null, url=null, language=null, rfNumber=[2], rfOrder=1, authorNames=null, journalName=Pharmaceutical Standards of the Ministry of Health of the People's Republic of China: Tibetan Medicine Volume 1(中华人民共和国卫生部药品标准:藏药第一册), refType=null, unstructuredReference=Pharmaceutical Standards of the Ministry of Health of the People's Republic of China: Tibetan Medicine Volume 1(中华人民共和国卫生部药品标准:藏药第一册)[S]. 1995:340., articleTitle=null, refAbstract=null), Reference(id=1249073243580207759, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601952666412025, doi=null, pmid=null, pmcid=null, year=2011, volume=null, issue=null, pageStart=284, pageEnd=287, url=null, language=null, rfNumber=[3], rfOrder=2, authorNames=null, journalName=Sichuan Provincial Standard for Traditional Chinese Medicine(四川省中药材标准), refType=null, unstructuredReference=Sichuan Provincial Standard for Traditional Chinese Medicine(四川省中药材标准)[S]. 2011:284-287., articleTitle=null, refAbstract=null), Reference(id=1249073243714425492, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601952666412025, doi=null, pmid=null, pmcid=null, year=2012, volume=null, issue=null, pageStart=54, pageEnd=null, url=null, language=null, rfNumber=[4], rfOrder=3, authorNames=null, journalName=Volume Ⅰ of the Standards for Tibetan Medicinal Materials in the Tibet Autonomous Region(西藏自治区藏药材标准第一册), refType=null, unstructuredReference=Volume Ⅰ of the Standards for Tibetan Medicinal Materials in the Tibet Autonomous Region(西藏自治区藏药材标准第一册)[S]. 2012:54., articleTitle=null, refAbstract=null), Reference(id=1249073243823477399, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601952666412025, doi=null, pmid=null, pmcid=null, year=2020, volume=null, issue=null, pageStart=107, pageEnd=113, url=null, language=null, rfNumber=[5], rfOrder=4, authorNames=null, journalName=Qinghai Provincial Standard for Tibetan Medicinal Materials(青海省藏药材标准), refType=null, unstructuredReference=Qinghai Provincial Standard for Tibetan Medicinal Materials(青海省藏药材标准)[S]. 2020:107-113., articleTitle=null, refAbstract=null), Reference(id=1249073243898974876, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601952666412025, doi=null, pmid=null, pmcid=null, year=2021, volume=52, issue=1, pageStart=211, pageEnd=218, url=null, language=null, rfNumber=[6], rfOrder=5, authorNames=TIAN R, WU Y, GU W, journalName=中草药, refType=null, unstructuredReference=TIAN R, WU Y, GU W, et al. Molecular identification of Erythrinae Cortex and its adulterants based on ITS2 sequence[J]. Chin Tradit Herb Drugs(中草药), 2021, 52(1):211-218., articleTitle=Molecular identification of Erythrinae Cortex and its adulterants based on ITS2 sequence, refAbstract=null), Reference(id=1249073243995443869, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601952666412025, doi=null, pmid=null, pmcid=null, year=2003, volume=270, issue=1512, pageStart=313, pageEnd=321, url=null, language=null, rfNumber=[7], rfOrder=6, authorNames=HEBERT P D N, CYWINSKA A, BALL S L, journalName=Proc R Soc Lond B, refType=null, unstructuredReference=HEBERT P D N, CYWINSKA A, BALL S L, et al. Biological identifications through DNA barcodes[J]. Proc R Soc Lond B, 2003, 270(1512):313-321., articleTitle=et al. Biological identifications through DNA barcodes, refAbstract=null), Reference(id=1249073244087718559, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601952666412025, doi=null, pmid=null, pmcid=null, year=2022, volume=53, issue=21, pageStart=6857, pageEnd=6864, url=null, language=null, rfNumber=[8], rfOrder=7, authorNames=GAO B X, QI J L, ZHONG H, journalName=中草药, refType=null, unstructuredReference=GAO B X, QI J L, ZHONG H, et al. Identification of commonly used origins of Tibetan medicine Lagotidis Herba based on ITS2 barcode and market analysis[J]. Chin Tradit Herb Drugs(中草药), 2022, 53(21):6857-6864., articleTitle=Identification of commonly used origins of Tibetan medicine Lagotidis Herba based on ITS2 barcode and market analysis, refAbstract=null), Reference(id=1249073244188381858, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601952666412025, doi=null, pmid=null, pmcid=null, year=2021, volume=23, issue=5, pageStart=786, pageEnd=791, url=null, language=null, rfNumber=[9], rfOrder=8, authorNames=QI J L, GAO B X, GOU Y, journalName=中国现代中药, refType=null, unstructuredReference=QI J L, GAO B X, GOU Y, et al. Identification of zanthoxylum armatum and its closely related species based on ITS2 sequence[J]. Mod Chin Med(中国现代中药), 2021, 23(5):786-791,814., articleTitle=Identification of zanthoxylum armatum and its closely related species based on ITS2 sequence, refAbstract=null), Reference(id=1249073244276462244, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601952666412025, doi=null, pmid=null, pmcid=null, year=2020, volume=43, issue=6, pageStart=1336, pageEnd=1342, url=null, language=null, rfNumber=[10], rfOrder=9, authorNames=SUN S Y, CHEN Y, WANG Q R, journalName=中药材, refType=null, unstructuredReference=SUN S Y, CHEN Y, WANG Q R, et al. Molecular Identification of Lysimachia christinae and its Substitute andAdulterants based on the ITS2 and rbcL Barcodes[J]. J Chin Med Mater(中药材), 2020, 43(6):1336-1342., articleTitle=Molecular Identification of Lysimachia christinae and its Substitute andAdulterants based on the ITS2 and rbcL Barcodes, refAbstract=null), Reference(id=1249073244381319848, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601952666412025, doi=null, pmid=null, pmcid=null, year=2018, volume=49, issue=24, pageStart=5901, pageEnd=5909, url=null, language=null, rfNumber=[11], rfOrder=10, authorNames=LIU Q, GU W, YANG B, journalName=中药材, refType=null, unstructuredReference=LIU Q, GU W, YANG B, et al. Molecular identification of Cynanchum auriculatum and its closely related speciesbased on ITS2 sequence[J]. J Chin Med Mater(中药材), 2018, 49(24):5901-5909., articleTitle=Molecular identification of Cynanchum auriculatum and its closely related speciesbased on ITS2 sequence, refAbstract=null), Reference(id=1249073244465205931, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601952666412025, doi=null, pmid=null, pmcid=null, year=2023, volume=58, issue=3, pageStart=231, pageEnd=235, url=null, language=null, rfNumber=[12], rfOrder=11, authorNames=CHENG B, DILINUER T, YU L T, journalName=中国药学杂志, refType=null, unstructuredReference=CHENG B, DILINUER T, YU L T, et al. Identification of uighur medicine ribes nigrum and its adulterants based onInternal transcribed spacer 2[J]. Chin Pharm J(中国药学杂志), 2023, 58(3):231-235., articleTitle=Identification of uighur medicine ribes nigrum and its adulterants based onInternal transcribed spacer 2, refAbstract=null), Reference(id=1249073244574257839, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601952666412025, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[13], rfOrder=12, authorNames=null, journalName=null, refType=null, unstructuredReference=Ch.P (2020) VolⅣ(中国药典2020年版.四部) [S]. 2020:126., articleTitle=null, refAbstract=null), Reference(id=1249073244645561010, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601952666412025, doi=null, pmid=null, pmcid=null, year=2015, volume=null, issue=null, pageStart=23, pageEnd=null, url=null, language=null, rfNumber=[14], rfOrder=13, authorNames=CHEN S L, journalName=Standard DNA Barcodes of Chinese Materia Medica in Chinese Pharmacopoeia(中国药典中药材DNA条形码标准序列), refType=null, unstructuredReference=CHEN S L. Standard DNA Barcodes of Chinese Materia Medica in Chinese Pharmacopoeia(中国药典中药材DNA条形码标准序列)[M]. Beijing: Science Press, 2015: 23., articleTitle=null, refAbstract=null)], funds=[Fund(id=1249073243135611523, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601952666412025, awardId=RS2024Z006, language=CN, fundingSource=国家药品监督管理局药品监管科学体系建设重点项目资助(RS2024Z006), fundOrder=null, country=null)], companyList=[AuthorCompany(id=1249073235061576102, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601952666412025, xref=1, ext=[AuthorCompanyExt(id=1249073235069964711, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601952666412025, companyId=1249073235061576102, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1 Key Laboratory of Quality Evaluation of Chinese Patent Medicines, Sichuan Institute for Drug Control, NMPA, Chengdu 611731, China), AuthorCompanyExt(id=1249073235078353320, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601952666412025, companyId=1249073235061576102, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1 四川省药品检验研究院, 国家药品监督管理局中成药质量评价重点实验室, 成都 611731)]), AuthorCompany(id=1249073235162239401, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601952666412025, xref=2, ext=[AuthorCompanyExt(id=1249073235170628010, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601952666412025, companyId=1249073235162239401, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2 Luzhou College of Medical Devices, Luzhou 646000, China), AuthorCompanyExt(id=1249073235183210923, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601952666412025, companyId=1249073235162239401, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2 泸州医疗器械职业学院, 四川 泸州 646000)]), AuthorCompany(id=1249073235246125484, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601952666412025, xref=3, ext=[AuthorCompanyExt(id=1249073235254514093, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601952666412025, companyId=1249073235246125484, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=3 College of Pharmacy, Chengdu University of Traditional Chinese Medicine, State Key Laboratory of Traditional Chinese Medicine Resources of Southwestern China, Key Laboratory of Standardization of Chinese Herbal Medicine, Chengdu 611137, China), AuthorCompanyExt(id=1249073235262902702, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601952666412025, companyId=1249073235246125484, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=3 成都中医药大学药学院, 西南特色中药资源国家重点实验室,中药材标准化教育部重点实验室, 四川 成都 611137)])], figs=[ArticleFig(id=1249073239696282161, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601952666412025, language=EN, label=Fig.1, caption=ITS2 electrophoretic diagram of Tibetan medicine Jiawa and its easily mixed species, figureFileSmall=THSCwg2UJ1qTophhD3TqLg==, figureFileBig=JS7V3YvQ3ev5tVqClzfIfg==, tableContent=null), ArticleFig(id=1249073239805334067, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601952666412025, language=CN, label=图1, caption=加哇及其易混品ITS2电泳图, figureFileSmall=THSCwg2UJ1qTophhD3TqLg==, figureFileBig=JS7V3YvQ3ev5tVqClzfIfg==, tableContent=null), ArticleFig(id=1249073239901803060, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601952666412025, language=EN, label=Fig.2, caption=Phylogenetic tree constructed with ITS2 sequences using N-J method, figureFileSmall=C6Z0NJp3e9DWb0RwPwwH1A==, figureFileBig=dITq154roxXr18c289wEPQ==, tableContent=null), ArticleFig(id=1249073240006660663, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601952666412025, language=CN, label=图2, caption=基于ITS2序列构建的邻接法(N-J)系统聚类树, figureFileSmall=C6Z0NJp3e9DWb0RwPwwH1A==, figureFileBig=dITq154roxXr18c289wEPQ==, tableContent=null), ArticleFig(id=1249073240119906879, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601952666412025, language=EN, label=Fig.3, caption=ITS2 secondary structure, figureFileSmall=22ejPKxmTUVxKzxKikRpNw==, figureFileBig=Pnr7ma0NW17bUC5hilXgqA==, tableContent=null), ArticleFig(id=1249073240216375877, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601952666412025, language=CN, label=图3, caption=ITS2二级结构, figureFileSmall=22ejPKxmTUVxKzxKikRpNw==, figureFileBig=Pnr7ma0NW17bUC5hilXgqA==, tableContent=null), ArticleFig(id=1249073241785045582, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601952666412025, language=EN, label=Tab.1, caption=

Information of Tibetan medicine Jiawa and its easily mixed species

, figureFileSmall=null, figureFileBig=null, tableContent=
No. Origin Collection place
(in Chinese)		 Method of
collecting
XZ-1 Pleurospermum hookeri
var. thomsonii C. B.
Clarke		 Shiqu Sichuan(四川石渠) Pick
XZ-2 Kangding Sichuan(四川康定) Pick
XZ-3 Yajiang Sichuan(四川雅江) Pick
XZ-4 Daocheng Sichuan(四川稻城) Pick
XZ-5 Dege Sichuan(四川德格) Pick
XZ-6 Dege Sichuan(四川德格) Pick
XZ-7 Shiqu Sichuan(四川石渠) Pick
XZ-8 Shiqu Sichuan(四川石渠) Pick
XZ-9 Ganzi Sichuan(四川甘孜) Pick
XZ-10 Seda Sichuan(四川色达) Pick
XZ-11 Xiaojin Sichuan(四川小金) Pick
XZ-12 Gande Qinghai(青海甘德) Pick
XZ-13 Gande Qinghai(青海甘德) Pick
XZ-14 Maqin Qinghai(青海玛沁) Pick
XZ-15 Litang Sichuan(四川理塘) Pick
MGQ-1 Sphallerocarpus gracilis
(Bess.) K.-Pol.		 Xining Qinghai(青海西宁) Purchase
MGQ-2 Xining Qinghai(青海西宁) Purchase
MGQ-3 Xining Qinghai(青海西宁) Purchase
MGQ-4 Maqin Qinghai(青海玛沁) Purchase
MGQ-5 Nangqian Qinghai(青海囊谦) Purchase
MGQ-6 Tongren Qinghai(青海同仁) Purchase
MGQ-7 Yushu Qinghai(青海玉树) Purchase
MGQ-8 Lasa Xizang(西藏拉萨) Purchase
MGQ-9 Chengdu Sichuan(四川成都) Purchase
MGQ-10 Chengdu Sichuan(四川成都) Purchase
MGQ-11 Maqin Qinghai(青海玛沁) Purchase
MGQ-12 Shandan Gansu(甘肃山丹) Pick
MGQ-13 Minle Gansu(甘肃民乐) Pick
MGQ-14 Chengdu Sichuan(四川成都) Purchase
MGQ-15 Zhangye Gansu(甘肃张掖) Purchase
MGQ-16 Hongyuan Sichuan(四川红原) Pick
ARQ-1 Vicatia thibetica de
Boiss.		 Xichang Sichuan(四川西昌) Purchase
ARQ-2 Panzhihua Sichuan(四川攀枝花) Pick
ARQ-3 Huidong Sichuan(四川会东) Purchase
ARQ-4 Zhanyi Yunan(云南沾益) Pick
ARQ-5 Kunming Yunan(云南昆明) Pick
ARQ-6 Qujing Yunan(云南曲靖) Purchase
ARQ-7 Huidong Sichuan(四川会东) Pick
ARQ-8 Huize Yunan(云南会泽) Pick
ARQ-9 Huidong Sichuan(四川会东) Pick
ARQ-10 Mojiang Yunan(云南墨江) Pick
ARQ-11 Yanyuan Sichuan(四川盐源) Pick
ARQ-12 Chengdu Sichuan(四川成都) Purchase
ARQ-13 Chengdu Sichuan(四川成都) Purchase
ARQ-14 DaliYunan(云南大理) Purchase
ARQ-15 Aba Sichuan(四川阿坝) Purchase
ARQ-16 Huidong Sichuan(四川会东) Pick
GLZ-1 Carum carvi L. Ganzi Sichuan(四川甘孜) Pick
GLZ-2 Ganzi Sichuan(四川甘孜) Pick
GLZ-3 Aba Sichuan(四川阿坝) Pick
GLZ-4 Aba Sichuan(四川阿坝) Pick
GLZ-5 Maqin Qinghai(青海玛沁) Pick
GLZ-6 Banma Qinghai(青海班玛) Pick
SP-1 Pleurospermum franche-
tianum Hemsl.		 Yajiang Sichuan(四川雅江) Pick
SP-2 Maerkang Sichuan(四川马尔康) Pick
SP-3 Hongyuan Sichuan(四川红原) Pick
SP-4 Songpan Sichuan(四川松潘) Pick
SP-5 Xiaojin Sichuan(四川小金) Pick
LY-1 Heracleum millefolium
Diels		 Shiqu Sichuan(四川石渠) Pick
LY-2 Ruoergai Sichuan(四川若尔盖) Pick
LY-3 Gande Qinghai(青海甘德) Pick
LY-4 Shiqu Sichuan(四川石渠) Pick
YB-1 Ligusticum daucoides
(Franch.) Franch.		 Litang Sichuan(四川理塘) Pick
YB-2 Luding Sichuan(四川泸定) Pick
YB-3 Yajiang Sichuan(四川雅江) Pick
YB-4 Daocheng Sichuan(四川稻城) Pick
YB-5 Shiqu Sichuan(四川石渠) Pick
YB-6 Maerkang Sichuan(四川马尔康) Pick
CG-1 Anthriscus sylvestris
subsp. Nemorosa		 Baiyu Sichuan(四川白玉) Pick
CG-2 Baiyu Sichuan(四川白玉) Pick
LG-1 Pleurospermum wrigh-
tianum de Boiss.		 Yajiang Sichuan(四川雅江) Pick
LG-2 Shiqu Sichuan(四川石渠) Pick
), ArticleFig(id=1249073241877320272, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601952666412025, language=CN, label=表1, caption=

藏药加哇及其易混品样品信息

, figureFileSmall=null, figureFileBig=null, tableContent=
No. Origin Collection place
(in Chinese)		 Method of
collecting
XZ-1 Pleurospermum hookeri
var. thomsonii C. B.
Clarke		 Shiqu Sichuan(四川石渠) Pick
XZ-2 Kangding Sichuan(四川康定) Pick
XZ-3 Yajiang Sichuan(四川雅江) Pick
XZ-4 Daocheng Sichuan(四川稻城) Pick
XZ-5 Dege Sichuan(四川德格) Pick
XZ-6 Dege Sichuan(四川德格) Pick
XZ-7 Shiqu Sichuan(四川石渠) Pick
XZ-8 Shiqu Sichuan(四川石渠) Pick
XZ-9 Ganzi Sichuan(四川甘孜) Pick
XZ-10 Seda Sichuan(四川色达) Pick
XZ-11 Xiaojin Sichuan(四川小金) Pick
XZ-12 Gande Qinghai(青海甘德) Pick
XZ-13 Gande Qinghai(青海甘德) Pick
XZ-14 Maqin Qinghai(青海玛沁) Pick
XZ-15 Litang Sichuan(四川理塘) Pick
MGQ-1 Sphallerocarpus gracilis
(Bess.) K.-Pol.		 Xining Qinghai(青海西宁) Purchase
MGQ-2 Xining Qinghai(青海西宁) Purchase
MGQ-3 Xining Qinghai(青海西宁) Purchase
MGQ-4 Maqin Qinghai(青海玛沁) Purchase
MGQ-5 Nangqian Qinghai(青海囊谦) Purchase
MGQ-6 Tongren Qinghai(青海同仁) Purchase
MGQ-7 Yushu Qinghai(青海玉树) Purchase
MGQ-8 Lasa Xizang(西藏拉萨) Purchase
MGQ-9 Chengdu Sichuan(四川成都) Purchase
MGQ-10 Chengdu Sichuan(四川成都) Purchase
MGQ-11 Maqin Qinghai(青海玛沁) Purchase
MGQ-12 Shandan Gansu(甘肃山丹) Pick
MGQ-13 Minle Gansu(甘肃民乐) Pick
MGQ-14 Chengdu Sichuan(四川成都) Purchase
MGQ-15 Zhangye Gansu(甘肃张掖) Purchase
MGQ-16 Hongyuan Sichuan(四川红原) Pick
ARQ-1 Vicatia thibetica de
Boiss.		 Xichang Sichuan(四川西昌) Purchase
ARQ-2 Panzhihua Sichuan(四川攀枝花) Pick
ARQ-3 Huidong Sichuan(四川会东) Purchase
ARQ-4 Zhanyi Yunan(云南沾益) Pick
ARQ-5 Kunming Yunan(云南昆明) Pick
ARQ-6 Qujing Yunan(云南曲靖) Purchase
ARQ-7 Huidong Sichuan(四川会东) Pick
ARQ-8 Huize Yunan(云南会泽) Pick
ARQ-9 Huidong Sichuan(四川会东) Pick
ARQ-10 Mojiang Yunan(云南墨江) Pick
ARQ-11 Yanyuan Sichuan(四川盐源) Pick
ARQ-12 Chengdu Sichuan(四川成都) Purchase
ARQ-13 Chengdu Sichuan(四川成都) Purchase
ARQ-14 DaliYunan(云南大理) Purchase
ARQ-15 Aba Sichuan(四川阿坝) Purchase
ARQ-16 Huidong Sichuan(四川会东) Pick
GLZ-1 Carum carvi L. Ganzi Sichuan(四川甘孜) Pick
GLZ-2 Ganzi Sichuan(四川甘孜) Pick
GLZ-3 Aba Sichuan(四川阿坝) Pick
GLZ-4 Aba Sichuan(四川阿坝) Pick
GLZ-5 Maqin Qinghai(青海玛沁) Pick
GLZ-6 Banma Qinghai(青海班玛) Pick
SP-1 Pleurospermum franche-
tianum Hemsl.		 Yajiang Sichuan(四川雅江) Pick
SP-2 Maerkang Sichuan(四川马尔康) Pick
SP-3 Hongyuan Sichuan(四川红原) Pick
SP-4 Songpan Sichuan(四川松潘) Pick
SP-5 Xiaojin Sichuan(四川小金) Pick
LY-1 Heracleum millefolium
Diels		 Shiqu Sichuan(四川石渠) Pick
LY-2 Ruoergai Sichuan(四川若尔盖) Pick
LY-3 Gande Qinghai(青海甘德) Pick
LY-4 Shiqu Sichuan(四川石渠) Pick
YB-1 Ligusticum daucoides
(Franch.) Franch.		 Litang Sichuan(四川理塘) Pick
YB-2 Luding Sichuan(四川泸定) Pick
YB-3 Yajiang Sichuan(四川雅江) Pick
YB-4 Daocheng Sichuan(四川稻城) Pick
YB-5 Shiqu Sichuan(四川石渠) Pick
YB-6 Maerkang Sichuan(四川马尔康) Pick
CG-1 Anthriscus sylvestris
subsp. Nemorosa		 Baiyu Sichuan(四川白玉) Pick
CG-2 Baiyu Sichuan(四川白玉) Pick
LG-1 Pleurospermum wrigh-
tianum de Boiss.		 Yajiang Sichuan(四川雅江) Pick
LG-2 Shiqu Sichuan(四川石渠) Pick
), ArticleFig(id=1249073241965400660, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601952666412025, language=EN, label=Tab.2, caption=

GenBank login number information of ITS2 sequence

, figureFileSmall=null, figureFileBig=null, tableContent=
Name GenBank login number
Pleurospermum hookeri var. thomsonii C.B.Clarke EU236199.1、AF164863.1、FJ483485.1、HQ824799.1、KP311520.1
Sphallerocarpus gracilis (Bess.) K. -Pol. KM191322.1、AH008959.2
Vicatia thibetica de Boiss. FJ483507.1、MN689000.1、GU395143.1
Carum carvi L. KF454471.1、MF785658.1、U78437.1、KX167447.1、KX167445.1、KM887355.1、KX167448.1
Pleurospermum franchetianum Hemsl. EU236198.1、KY848849.1
Heracleum millefolium Diels HQ686349.1、HQ686341.1、HQ686332.1、HQ686331.1、EU236170.1、EU001362.1、GU967798.1
Ligusticum daucoides (Franch.) Franch. EU236173.1
Anthriscus sylvestris subsp. Nemorosa AH008907.2
Pleurospermum wrightianum de Boiss. FJ483494.1、EU236201.1
), ArticleFig(id=1249073242053481049, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601952666412025, language=CN, label=表2, caption=

核基因内部转录间隔区(ITS2)序列的GenBank登录号信息

, figureFileSmall=null, figureFileBig=null, tableContent=
Name GenBank login number
Pleurospermum hookeri var. thomsonii C.B.Clarke EU236199.1、AF164863.1、FJ483485.1、HQ824799.1、KP311520.1
Sphallerocarpus gracilis (Bess.) K. -Pol. KM191322.1、AH008959.2
Vicatia thibetica de Boiss. FJ483507.1、MN689000.1、GU395143.1
Carum carvi L. KF454471.1、MF785658.1、U78437.1、KX167447.1、KX167445.1、KM887355.1、KX167448.1
Pleurospermum franchetianum Hemsl. EU236198.1、KY848849.1
Heracleum millefolium Diels HQ686349.1、HQ686341.1、HQ686332.1、HQ686331.1、EU236170.1、EU001362.1、GU967798.1
Ligusticum daucoides (Franch.) Franch. EU236173.1
Anthriscus sylvestris subsp. Nemorosa AH008907.2
Pleurospermum wrightianum de Boiss. FJ483494.1、EU236201.1
), ArticleFig(id=1249073242204475999, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601952666412025, language=EN, label=Tab.3, caption=

The ITS2 sequence information of Tibetan medicine Jiawa and its easily mixed species

, figureFileSmall=null, figureFileBig=null, tableContent=
Name Sequence number Sequence length/bp GC/%
Pleurospermum hookeri var. thomsonii C.B.Clarke 20 222 55.8-56.3
Sphallerocarpus gracilis (Bess.) K. -Pol. 18 225 49.3
Vicatia thibetica de Boiss. 19 219 50.2-52.5
Carum carvi L. 13 223 51.6
Pleurospermum franchetianum Hemsl. 7 216 56.0-56.9
Heracleum millefolium Diels 11 224 56.2
Ligusticum daucoides (Franch.) Franch. 7 221 55.7-58.4
Anthriscus sylvestris subsp. Nemorosa 3 222~225 47.5-48.0
Pleurospermum wrightianum de Boiss. 4 218~220 56.4-56.8
), ArticleFig(id=1249073242288362084, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601952666412025, language=CN, label=表3, caption=

藏药加哇及其易混品ITS2序列信息

, figureFileSmall=null, figureFileBig=null, tableContent=
Name Sequence number Sequence length/bp GC/%
Pleurospermum hookeri var. thomsonii C.B.Clarke 20 222 55.8-56.3
Sphallerocarpus gracilis (Bess.) K. -Pol. 18 225 49.3
Vicatia thibetica de Boiss. 19 219 50.2-52.5
Carum carvi L. 13 223 51.6
Pleurospermum franchetianum Hemsl. 7 216 56.0-56.9
Heracleum millefolium Diels 11 224 56.2
Ligusticum daucoides (Franch.) Franch. 7 221 55.7-58.4
Anthriscus sylvestris subsp. Nemorosa 3 222~225 47.5-48.0
Pleurospermum wrightianum de Boiss. 4 218~220 56.4-56.8
), ArticleFig(id=1249073242397413994, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601952666412025, language=EN, label=Tab.4, caption=

Statistics of variation sites of Tibetan medicine Jiawa and its easily mixed species

, figureFileSmall=null, figureFileBig=null, tableContent=
Name Variation
site		 Haplotype
Number of haplotypes Dominant haplotype(number) Experimental sample haplotype
Pleurospermum hookeri var. thomsonii C.B.Clarke 5 5 A1(11) A1、A2、A3
Sphallerocarpus gracilis (Bess.) K.-Pol. 0 1 B1 B1
Vicatia thibetica de Boiss. 5 4 C1(9) C1、C2
Carum carvi L. 0 1 D1 D1
Pleurospermum franchetianum Hemsl. 2 3 E1(5) E1、E2
Heracleum millefolium Diels 6 3 F1(5)、F2(5) F1、F2
Ligusticum daucoides (Franch.) Franch. 9 5 G1(3) G1、G2
Anthriscus sylvestris subsp. Nemorosa 1 2 H1(2) H1
Pleurospermum wrightianum de Boiss. 2 2 I1(3) I1、I2
), ArticleFig(id=1249073242560991855, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601952666412025, language=CN, label=表4, caption=

藏药加哇及其易混品变异位点统计

, figureFileSmall=null, figureFileBig=null, tableContent=
Name Variation
site		 Haplotype
Number of haplotypes Dominant haplotype(number) Experimental sample haplotype
Pleurospermum hookeri var. thomsonii C.B.Clarke 5 5 A1(11) A1、A2、A3
Sphallerocarpus gracilis (Bess.) K.-Pol. 0 1 B1 B1
Vicatia thibetica de Boiss. 5 4 C1(9) C1、C2
Carum carvi L. 0 1 D1 D1
Pleurospermum franchetianum Hemsl. 2 3 E1(5) E1、E2
Heracleum millefolium Diels 6 3 F1(5)、F2(5) F1、F2
Ligusticum daucoides (Franch.) Franch. 9 5 G1(3) G1、G2
Anthriscus sylvestris subsp. Nemorosa 1 2 H1(2) H1
Pleurospermum wrightianum de Boiss. 2 2 I1(3) I1、I2
), ArticleFig(id=1249073242699403893, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601952666412025, language=EN, label=Tab.5, caption=

Analysis of intraspecific and interspecific genetic distance

, figureFileSmall=null, figureFileBig=null, tableContent=
Name Pleurospermum
hookeri var.
thomsonii
C. B. Clarke		 Sphallerocarpus
gracilis
(Bess.)
K. -Pol.		 Vicatia
thibetica
de Boiss.		 Anthriscus sylvestris
subsp.
Nemorosa		 Carum
carvi L.		 Pleurospermum
franchetianum
Hemsl.		 Heracleum
millefolium
Diels		 Ligusticum
daucoides
(Franch.)
Franch.		 Pleurospermum
wrightianum
de Boiss.
Pleurospermum hookeri var. thomsonii C. B. Clarke (0-0.014)
0.004		 (0.201-0.214)
0.180		 (0.179-0.204)
0.169		 (0.205-0.218)
0.210		 (0.251-0.265)
0.239		 (0.358-0.384)
0.363		 (0.256-0.284)
0.258		 (0.206-0.226)
0.214		 (0.367-0.376)
0.361
Sphallerocarpus gracilis
(Bess.) K. -Pol.		 (0.201-0.214)
0.180		 0 (0.287-0.295)
0.243		 (0.148-0.154)
0.147		 (0.391-0.391)
0.363		 (0.425-0.442)
0.413		 (0.333-0.366)
0.307		 (0.253-0.296)
0.250		 (0.430-0.440)
0.398
Vicatia thibetica de Boiss. (0.179-0.204)
0.169		 (0.287-0.295)
0.243		 (0-0.023)
0.009		 0.283-0.298)
0.267		 (0.218-0.231)
0.213		 (0.308-0.357)
0.332		 (0.193-0.252)
0.217		 (0.156-0.212)
0.180		 (0.305-0.346)
0.327
Anthriscus sylvestris subsp.
Nemorosa		 (0.205-0.218)
0.210		 (0.148-0.154)
0.147		 (0.283-0.298)
0.267		 (0-0.005)
0.003		 (0.366-0.366)
0.345		 (0.462-0.483)
0.454		 (0.347-0.381)
0.347		 (0.265-0.293)
0.283		 (0.450-0.471)
0.440
Carum carvi L. (0.251-0.265)
0.239		 (0.391-0.391)
0.363		 (0.218-0.231)
0.213		 (0.366-0.366)
0.345		 0 (0.359-0.367)
0.370		 (0.244-0.271)
0.247		 (0.252-0.265)
0.250		 (0.362-0.362)
0.366
Pleurospermum franchet-
ianum Hemsl.		 (0.358-0.384)
0.363		 (0.425-0.442)
0.413		 (0.308-0.357)
0.332		 (0.462-0.483)
0.454		 (0.359-0.367)
0.370		 (0.000-0.009)
0.003		 (0.311-0.350)
0.338		 (0.303-0.366)
0.335		 (0.024-0.0.34)
0.031
Heracleum millefolium
Diels		 (0.256-0.284)
0.258		 (0.333-0.366)
0.307		 (0.193-0.252)
0.217		 (0.347-0.381)
0.347		 (0.244-0.271)
0.247		 (0.311-0.350)
0.338		 (0.000-0.028)
0.008		 (0.144-0.173)
0.165		 (0.315-0.338)
0.346
Ligusticum daucoides
(Franch.) Franch.		 (0.206-0.226)
0.214		 (0.253-0.296)
0.250		 (0.156-0.212)
0.180		 (0.265-0.293)
0.283		 (0.252-0.265)
0.250		 (0.303-0.366)
0.335		 (0.144-0.173)
0.165		 (0-0.033)
0.014		 (0.309-0.365)
0.352
Pleurospermum wrightia-
num de Boiss.		 (0.367-0.376)
0.361		 (0.430-0.440)
0.398		 (0.305-0.346)
0.327		 (0.450-0.471)
0.440		 (0.362-0.362)
0.366		 (0.024-0.0.34)
0.031		 (0.315-0.338)
0.346		 (0.309-0.365)
0.352		 (0-0.009)
0.005
), ArticleFig(id=1249073242800067192, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601952666412025, language=CN, label=表5, caption=

种内种间遗传距离分析

, figureFileSmall=null, figureFileBig=null, tableContent=
Name Pleurospermum
hookeri var.
thomsonii
C. B. Clarke		 Sphallerocarpus
gracilis
(Bess.)
K. -Pol.		 Vicatia
thibetica
de Boiss.		 Anthriscus sylvestris
subsp.
Nemorosa		 Carum
carvi L.		 Pleurospermum
franchetianum
Hemsl.		 Heracleum
millefolium
Diels		 Ligusticum
daucoides
(Franch.)
Franch.		 Pleurospermum
wrightianum
de Boiss.
Pleurospermum hookeri var. thomsonii C. B. Clarke (0-0.014)
0.004		 (0.201-0.214)
0.180		 (0.179-0.204)
0.169		 (0.205-0.218)
0.210		 (0.251-0.265)
0.239		 (0.358-0.384)
0.363		 (0.256-0.284)
0.258		 (0.206-0.226)
0.214		 (0.367-0.376)
0.361
Sphallerocarpus gracilis
(Bess.) K. -Pol.		 (0.201-0.214)
0.180		 0 (0.287-0.295)
0.243		 (0.148-0.154)
0.147		 (0.391-0.391)
0.363		 (0.425-0.442)
0.413		 (0.333-0.366)
0.307		 (0.253-0.296)
0.250		 (0.430-0.440)
0.398
Vicatia thibetica de Boiss. (0.179-0.204)
0.169		 (0.287-0.295)
0.243		 (0-0.023)
0.009		 0.283-0.298)
0.267		 (0.218-0.231)
0.213		 (0.308-0.357)
0.332		 (0.193-0.252)
0.217		 (0.156-0.212)
0.180		 (0.305-0.346)
0.327
Anthriscus sylvestris subsp.
Nemorosa		 (0.205-0.218)
0.210		 (0.148-0.154)
0.147		 (0.283-0.298)
0.267		 (0-0.005)
0.003		 (0.366-0.366)
0.345		 (0.462-0.483)
0.454		 (0.347-0.381)
0.347		 (0.265-0.293)
0.283		 (0.450-0.471)
0.440
Carum carvi L. (0.251-0.265)
0.239		 (0.391-0.391)
0.363		 (0.218-0.231)
0.213		 (0.366-0.366)
0.345		 0 (0.359-0.367)
0.370		 (0.244-0.271)
0.247		 (0.252-0.265)
0.250		 (0.362-0.362)
0.366
Pleurospermum franchet-
ianum Hemsl.		 (0.358-0.384)
0.363		 (0.425-0.442)
0.413		 (0.308-0.357)
0.332		 (0.462-0.483)
0.454		 (0.359-0.367)
0.370		 (0.000-0.009)
0.003		 (0.311-0.350)
0.338		 (0.303-0.366)
0.335		 (0.024-0.0.34)
0.031
Heracleum millefolium
Diels		 (0.256-0.284)
0.258		 (0.333-0.366)
0.307		 (0.193-0.252)
0.217		 (0.347-0.381)
0.347		 (0.244-0.271)
0.247		 (0.311-0.350)
0.338		 (0.000-0.028)
0.008		 (0.144-0.173)
0.165		 (0.315-0.338)
0.346
Ligusticum daucoides
(Franch.) Franch.		 (0.206-0.226)
0.214		 (0.253-0.296)
0.250		 (0.156-0.212)
0.180		 (0.265-0.293)
0.283		 (0.252-0.265)
0.250		 (0.303-0.366)
0.335		 (0.144-0.173)
0.165		 (0-0.033)
0.014		 (0.309-0.365)
0.352
Pleurospermum wrightia-
num de Boiss.		 (0.367-0.376)
0.361		 (0.430-0.440)
0.398		 (0.305-0.346)
0.327		 (0.450-0.471)
0.440		 (0.362-0.362)
0.366		 (0.024-0.0.34)
0.031		 (0.315-0.338)
0.346		 (0.309-0.365)
0.352		 (0-0.009)
0.005
), ArticleFig(id=1249073242892341882, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601952666412025, language=EN, label=Tab.6, caption=

Number of stem loops of Tibetan medicine Jiawa and its easily mixed species

, figureFileSmall=null, figureFileBig=null, tableContent=
Name Helix Ⅰ Helix Ⅱ Helix Ⅲ Helix Ⅳ
Pleurospermum hookeri var. thomsonii C.B.Clarke 2 2 5 1
Sphallerocarpus gracilis (Bess.) K.-Pol. 4 2 5 1
Vicatia thibetica de Boiss. 3 2 4 2
Anthriscus sylvestris subsp. Nemorosa 4 2 5 1
Carum carvi L. 3 2 6 1
Pleurospermum franchetianum Hemsl. 3 2 4 2
Heracleum millefolium Diels 3 2 3 1
Ligusticum daucoides (Franch.) Franch. 3 2 5 1
Pleurospermum wrightianum de Boiss. 2 2 5 2
), ArticleFig(id=1249073242967839360, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601952666412025, language=CN, label=表6, caption=

藏药加哇及其易混品茎环数目

, figureFileSmall=null, figureFileBig=null, tableContent=
Name Helix Ⅰ Helix Ⅱ Helix Ⅲ Helix Ⅳ
Pleurospermum hookeri var. thomsonii C.B.Clarke 2 2 5 1
Sphallerocarpus gracilis (Bess.) K.-Pol. 4 2 5 1
Vicatia thibetica de Boiss. 3 2 4 2
Anthriscus sylvestris subsp. Nemorosa 4 2 5 1
Carum carvi L. 3 2 6 1
Pleurospermum franchetianum Hemsl. 3 2 4 2
Heracleum millefolium Diels 3 2 3 1
Ligusticum daucoides (Franch.) Franch. 3 2 5 1
Pleurospermum wrightianum de Boiss. 2 2 5 2
)], attaches=null, journal=Journal(id=1190317596361715715, delFlag=0, nameCn=中国药学杂志, nameEn=Chinese Pharmaceutical Journal, nameHistory1=null, nameHistory2=null, issn=1001-2494, eissn=null, cn=11-2162/R, coden=null, periodic=3, language=CN, oaType=null, ccby=null, superviseOffice=null, ownerOffice=null, pubOffice=null, editorOffice=null, officeType=null, aims=null, clcCode=null, officeProv=null, officeCity=null, officeAddr=null, officeZip=null, officeEmail=null, officePhone=null, editDirector=null, officeDirector=null, officeDirectorPhone=null, officeStaffNum=null, officeEmpNum=null, coverPicUrl=hRN1R6HnoNwYkve/JRn0DA==, journalPrice=null, startedYear=null, abbrevIsoEn=null, journalRemark=null, publicationField=null, createdTime=1761723430007, updatedTime=1761735858241, createdBy=18614031015, updatedBy=13701087609, firstLetterCn=C, firstLetterEn=C, subjectCode=Life Sciences, subjectName=Life Sciences, subjectCodeEn=Life Sciences, subjectNameEn=null, picCn=hRN1R6HnoNwYkve/JRn0DA==, picEn=xSRntM4yOh2wVIE2w+OjYg==, jcr=null, cjcr=null, exts=[JournalExt(id=1190369724262355196, language=CN, name=中国药学杂志, nameHistory1=null, nameHistory2=null, managedBy=, sponsoredBy=, publishedBy=, editorOffice=, officeProv=null, officeCity=null, officeAddr=, officeZip=, editDirector=, officeDirector=null, officePhone=null, coverPicUrl=null, journalRemark=, submitArticleUrl=null, websiteUrl=, createdTime=1761735858264, updatedTime=1761735858264, createdBy=13701087609, updatedBy=13701087609, submissionGuidelinesUrl=, submissionAuthorUrl=https://zgyxzzauthor.manuscriptcloud.com/login, submissionEditorUrl=https://zgyxzzeditor.manuscriptcloud.com/login, submissionReviewUrl=https://zgyxzzauthor.manuscriptcloud.com/login, submissionCeEditorUrl=, submissionAeEditorUrl=, option={"copyright":""}), JournalExt(id=1190369724358824189, language=EN, name=Chinese Pharmaceutical Journal, nameHistory1=null, nameHistory2=null, managedBy=, sponsoredBy=, publishedBy=, editorOffice=, officeProv=null, officeCity=null, officeAddr=, officeZip=, editDirector=, officeDirector=null, officePhone=null, coverPicUrl=null, journalRemark=, submitArticleUrl=null, websiteUrl=, createdTime=1761735858287, updatedTime=1761735858287, createdBy=13701087609, updatedBy=13701087609, submissionGuidelinesUrl=, submissionAuthorUrl=https://zgyxzzauthor.manuscriptcloud.com/login, submissionEditorUrl=https://zgyxzzeditor.manuscriptcloud.com/login, submissionReviewUrl=https://zgyxzzauthor.manuscriptcloud.com/login, submissionCeEditorUrl=, submissionAeEditorUrl=, option={"copyright":""})], databaseList=null, tenantJournalId=1190317699101192196, websiteList=[Website(id=1190317834875011552, webName=null, webTitle=null, webDomain=null, webCopyrigh=null, webIpcNo=null, seoTitle=null, seoKeywords=null, seoDescription=null, tenantJournalId=null, journalId=1190317699101192196, journalNameCn=null, journalNameEn=null, grayFlag=null, tenantId=1146029695717560320, platformId=null, journalGroupId=null, journalGroupNameCn=null, journalGroupNameEn=null, type=1, domain=https://castjournals.cast.org.cn/joweb/zgyxzz/CN, language=CN, createTime=1761723486870, createBy=18614031015, updateTime=1761723510130, updateBy=18614031015, name=中国药学杂志-中文, tplId=1146099689490845704, title=中国药学杂志, delFlag=0, indexPage=/home, props=[WebsiteProps(id=1190318144041353703, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1190317834875011552, code=articleTextType, value=kx, createTime=1761723560581, updateTime=1761723560581, creator=18614031015, updator=18614031015), WebsiteProps(id=1190318144016187876, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1190317834875011552, code=banner, value=null, createTime=1761723560575, updateTime=1761723560575, creator=18614031015, updator=18614031015), WebsiteProps(id=1190318144062325226, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1190317834875011552, code=grayFlag, value=0, createTime=1761723560586, updateTime=1761723560586, creator=18614031015, updator=18614031015), WebsiteProps(id=1190318144007799267, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1190317834875011552, code=logo, value=https://castjournals.cast.org.cn/joweb/zgyxzz/CN/file/pic?fileId=puyAm9wIHqZks7K8hj8APQ==, createTime=1761723560573, updateTime=1761723560573, creator=18614031015, updator=18614031015), WebsiteProps(id=1190318144074908140, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1190317834875011552, code=minRunFlag, value=0, createTime=1761723560589, updateTime=1761723560589, creator=18614031015, updator=18614031015), WebsiteProps(id=1190318144032965094, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1190317834875011552, code=picServerUrl, value=https://castjournals.cast.org.cn/joweb/zgyxzz/CN/file/pic, createTime=1761723560579, updateTime=1761723560579, creator=18614031015, updator=18614031015), WebsiteProps(id=1190318144070713835, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1190317834875011552, code=silenceFlag, value=0, createTime=1761723560588, updateTime=1761723560588, creator=18614031015, updator=18614031015), WebsiteProps(id=1190318144024576485, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1190317834875011552, code=staticResourcePath, value=https://castjournals.cast.org.cn/joweb/cast_kjdb_cn_619/, createTime=1761723560577, updateTime=1761723560577, creator=18614031015, updator=18614031015), WebsiteProps(id=1190318144049742312, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1190317834875011552, code=themeColor, value=null, createTime=1761723560583, updateTime=1761723560583, creator=18614031015, updator=18614031015), WebsiteProps(id=1190318144053936617, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1190317834875011552, code=themeStyle, value=null, createTime=1761723560584, updateTime=1761723560584, creator=18614031015, updator=18614031015)]), Website(id=1190317834937926113, webName=null, webTitle=null, webDomain=null, webCopyrigh=null, webIpcNo=null, seoTitle=null, seoKeywords=null, seoDescription=null, tenantJournalId=null, journalId=1190317699101192196, journalNameCn=null, journalNameEn=null, grayFlag=null, tenantId=1146029695717560320, platformId=null, journalGroupId=null, journalGroupNameCn=null, journalGroupNameEn=null, type=1, domain=https://castjournals.cast.org.cn/joweb/zgyxzz/EN, language=EN, createTime=1761723486885, createBy=18614031015, updateTime=1761723527689, updateBy=18614031015, name=中国药学杂志-英文, tplId=1146101810881728533, title=Chinese Pharmaceutical Journal, delFlag=0, indexPage=/home, props=[WebsiteProps(id=1190318170478051825, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1190317834937926113, code=articleTextType, value=kx, createTime=1761723566884, updateTime=1761723566884, creator=18614031015, updator=18614031015), WebsiteProps(id=1190318170461274606, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1190317834937926113, code=banner, value=null, createTime=1761723566880, updateTime=1761723566880, creator=18614031015, updator=18614031015), WebsiteProps(id=1190318170494829044, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1190317834937926113, code=grayFlag, value=0, createTime=1761723566888, updateTime=1761723566888, creator=18614031015, updator=18614031015), WebsiteProps(id=1190318170452885997, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1190317834937926113, code=logo, value=https://castjournals.cast.org.cn/joweb/zgyxzz/EN/file/pic?fileId=puyAm9wIHqZks7K8hj8APQ==, createTime=1761723566878, updateTime=1761723566878, creator=18614031015, updator=18614031015), WebsiteProps(id=1190318170507411958, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1190317834937926113, code=minRunFlag, value=0, createTime=1761723566891, updateTime=1761723566891, creator=18614031015, updator=18614031015), WebsiteProps(id=1190318170473857520, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1190317834937926113, code=picServerUrl, value=https://castjournals.cast.org.cn/joweb/zgyxzz/EN/file/pic, createTime=1761723566883, updateTime=1761723566883, creator=18614031015, updator=18614031015), WebsiteProps(id=1190318170503217653, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1190317834937926113, code=silenceFlag, value=0, createTime=1761723566890, updateTime=1761723566890, creator=18614031015, updator=18614031015), WebsiteProps(id=1190318170465468911, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1190317834937926113, code=staticResourcePath, value=https://castjournals.cast.org.cn/joweb/cast_kjdb_en_623/, createTime=1761723566881, updateTime=1761723566881, creator=18614031015, updator=18614031015), WebsiteProps(id=1190318170482246130, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1190317834937926113, code=themeColor, value=null, createTime=1761723566885, updateTime=1761723566885, creator=18614031015, updator=18614031015), WebsiteProps(id=1190318170486440435, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1190317834937926113, code=themeStyle, value=null, createTime=1761723566886, updateTime=1761723566886, creator=18614031015, updator=18614031015)])], journalTitle=中国药学杂志, weixinUrl=null, journalUrl=http://www.zgyxzz.com.cn/, iacademicId=null, status=1, seqNo=null, journalTitleEn=Chinese Pharmaceutical Journal, journalPhotoCn=hRN1R6HnoNwYkve/JRn0DA==, journalPhotoEn=xSRntM4yOh2wVIE2w+OjYg==, journalFirstLetter=C, journalRecommend=null, journalNew=null, journalCollection=null, jcrJf=null, cjcrJf=null, jcrJfStr=null, cjcrJfStr=null, submissionFirstDecision=null, sciSubjectClassification=null, casSubjectClassification=null, citeScore=null, totalCitationFrequency=null, icpCode=null, psCode=null, advertisingLicenseCode=null, copyrightInformation=null, country=null, option=, provinceCode=null, provinceName=null, collectFlag=false), detailUrlCn=https://castjournals.cast.org.cn/joweb/zgyxzz/CN/Y2024/V59/I8/687, detailUrlEn=https://castjournals.cast.org.cn/joweb/zgyxzz/EN/Y2024/V59/I8/687, pdfUrlCn=https://castjournals.cast.org.cn/joweb/zgyxzz/CN/PDF/Y2024/V59/I8/687, pdfUrlEn=https://castjournals.cast.org.cn/joweb/zgyxzz/EN/PDF/Y2024/V59/I8/687, aliStartDate=null, aliEndDate=null, collectionFlag=false, citedCount=null, citedUrl=null, reference=null)
收藏切换
藏药加哇及其易混品的ITS2序列鉴定研究
收藏切换
PDF下载
杨吉玉 1, 2 , 高必兴 1, * , 齐景梁 1 , 王艺娱 3 , 李倩 1 , 连艳 3, *
中国药学杂志 | 论著 2024,59(8): 687-693
收起
收藏切换
中国药学杂志 | 论著 2024, 59(8): 687-693
藏药加哇及其易混品的ITS2序列鉴定研究
全屏
杨吉玉1, 2, 高必兴1, *, 齐景梁1, 王艺娱3, 李倩1, 连艳3, *
作者信息
  • 1 四川省药品检验研究院, 国家药品监督管理局中成药质量评价重点实验室, 成都 611731
  • 2 泸州医疗器械职业学院, 四川 泸州 646000
  • 3 成都中医药大学药学院, 西南特色中药资源国家重点实验室,中药材标准化教育部重点实验室, 四川 成都 611137

    • 杨吉玉,女,助教 研究方向:中药品种、质量与资源开发应用研究

通讯作者:

*高必兴,男,主管中药师 研究方向:中药及民族药质量研究 Tel:(028)87877143;
连艳,女,高级实验师研究方向:中药品种、品质与资源研究 Tel:(028)61800231
Identification of Tibetan Medicine Jiawa and Easily Mixed Species Based on ITS2 Barcode
Jiyu YANG1, 2, Bixing GAO1, *, Jingliang QI1, Yiyu WANG3, Qian LI1, Yan LIAN3, *
Affiliations
  • 1 Key Laboratory of Quality Evaluation of Chinese Patent Medicines, Sichuan Institute for Drug Control, NMPA, Chengdu 611731, China
  • 2 Luzhou College of Medical Devices, Luzhou 646000, China
  • 3 College of Pharmacy, Chengdu University of Traditional Chinese Medicine, State Key Laboratory of Traditional Chinese Medicine Resources of Southwestern China, Key Laboratory of Standardization of Chinese Herbal Medicine, Chengdu 611137, China
出版时间: 2024-04-22
文章导航
收藏切换
摘要
收起

目的 利用DNA条形码核基因内部转录间隔区(ITS2)序列,建立加哇及其易混品的有效鉴定方法。方法 采用试剂盒提取72批加哇及其易混品的DNA,针对其ITS2片段进行聚合酶链式反应(PCR)扩增并双向测序,获取ITS2序列信息,同时从GenBank下载相关序列30条,应用MEGA6.0软件对102条ITS2序列进行序列比对分析,计算种内和种间Kimura双参数遗传距离(K2-P),构建邻接法(neighbor-joining,N-J)系统进化树,并预测ITS2二级结构。结果 K2-P遗传距离分析显示,除松潘棱子芹与瘤果棱子芹外,西藏棱子芹、迷果芹、西藏凹乳芹、葛缕子、裂叶独活、羽苞藁本及刺果峨参具有明显的条形码间距。N-J树聚类分析显示,各物种均能以>50%的自展支持率各自聚为1支,单系性良好。ITS2二级结构分析显示,加哇与其易混品在4个螺旋区的茎环数目、大小、位置有所差异。结论 ITS2序列可用来鉴别加哇及其易混品,有利于加哇的质量控制及资源利用。

加哇  /  核基因内部转录间隔区序列  /  易混品  /  藏药

OBJECTIVE To establish an effective identification method for Jiawa and easily mixed species by using DNA barcoding ITS2 sequence. METHODS The total DNA of 72 Jiawa and its similar species samples were extracted by using the plant genomic DNA Extraction kit,PCR amplification and bidirectional sequencing were carried out for the ITS2 fragments, another 30 items of the genus were downloaded from GenBank. MEGA6.0 software was applied to analyze 102 ITS2 sequences, calculate the intraspecific and interspecific K2-P distance, construct neighber-jioning (N-J) phylogenetic tree, and predict the secondary structure of ITS2. RESULTS K2-P genetic distance analysis showed that, in addition to Pleurospermum franchetianum Hemsl. and Pleurospermum wrightianum de Boiss., Pleurospermum hookeri var. thomsonii C.B. Clarke., Sphallerocarpus gracilis (Bess.) K.-Pol., Vicatia thibetica de Boiss., Carum carvi L., Heracleum millefolium Diels., Ligusticum daucoides (Franch.) Franch. and Anthriscus sylvestris subsp. Nemorosa have obvious barcode spacing. N-J tree cluster analysis showed that each species could be clustered into one branch with a bootstrap support rate of >50%, and the monophyleticity was good. The secondary structure analysis of ITS2 showed that the number, size and position of stem-loops in the four helical regions of Jiawa and its miscible were different. CONCLUSION The ITS2 sequence can be used to identify Jiawa and its easily mixed species, which is beneficial to the rational development and utilization of Jiawa resources.

Jiawa  /  ITS2 barcode  /  mixed species  /  Tibetan medicine
杨吉玉, 高必兴, 齐景梁, 王艺娱, 李倩, 连艳. 藏药加哇及其易混品的ITS2序列鉴定研究. 中国药学杂志, 2024 , 59 (8) : 687 -693 .
Jiyu YANG, Bixing GAO, Jingliang QI, Yiyu WANG, Qian LI, Yan LIAN. Identification of Tibetan Medicine Jiawa and Easily Mixed Species Based on ITS2 Barcode[J]. Chinese Pharmaceutical Journal, 2024 , 59 (8) : 687 -693 .
正文
收起
加哇是一味常见藏药,藏文名“ ”,药用部位为根应用历史悠久,最早记载于《四部医典》[1],具有干黄水、祛腰肾寒症功效,主治消化不良,肾病,腰痛,肾虚,隆病,黄水病等。现收载于《中华人民共和国卫生部药品标准藏药分册》(1995年)[2]、《四川省中药材标准》(2010年版)[3]、《西藏自治区藏药材标准》第一册[4]及《青海省藏药材标准》(2019年版)第一册[5],主要收录西藏棱子芹(Pleurospermum hookeri var. thomsonii C. B. Clarke)及迷果芹(Sphallerocarpus gracilis (Bess.) K. -Pol.)两个基原。前期调查研究发现,五省藏区内加哇的使用基原远远不止以上两种,还包括西藏凹乳芹(Vicatia thibetica de Boiss.)、刺果峨参(Anthriscus sylvestris subsp. Nemorosa)等一系列基原,基原复杂,鉴定困难,表现于药材的鉴别更甚,目前传统鉴别方法难以将其区分。
DNA条形码是当前生物分类学的热门技术,是利用一段有足够变异的、易扩增且相对较短的标准DNA片段(500~1 000 bp)对物种进行快速、准确的自动鉴定[6]。DNA条形码自正式提出以来发展迅速[7],因其准确、快速、有效的特点,在中药及民族药领域使用广泛[8-12]。现有关于加哇的研究中未见有关DNA条形码的相关报道,课题组前期在四川省、青海省等地收集加哇样品,并采集了当地藏区常见易混品种,对其ITS2序列进行比对分析,找出藏药加哇在分子水平的鉴别特征,建立一种快速鉴别加哇及其易混品的方法,保障临床安全用药。
1 仪器及试药
收起
C1000型聚合酶链式反应(PCR)仪、PowerPac Basic型电泳仪、Gel Doc XR+型全自动凝胶成像系统(美国Bio-Rad公司);XP205型电子分析天平(瑞士Mettler Toledo公司);MM400型球磨仪(德国Retsch公司);MiniSpin型高速离心机(德国Eppendorf公司);Milli-Q Advantage A10型纯水仪(美国Millipore公司);植物基因组DNA提取试剂盒、2×Taq Master Mix缓冲液、Gene Red核酸染料(中国天根生化科技有限公司);Taq酶PCR扩增试剂盒(中国北京聚合美生物科技有限公司);琼脂糖(法国Biowest公司);ITS2引物(ITS2F:5'-ATGCGATACTTGGTGTGAAT-3';ITS3R:5'-GACGCTTCTCCAGACTACAAT-3',中国上海捷瑞生物工程有限公司)。
收集加哇相关样品共72批,其中自采52批,购买20批,药材经四川省药品检验研究院黎跃成主任中药师鉴定,包括9个基原,分别为西藏棱子芹(Pleurospermum hookeri var. thomsonii C. B. Clarke)、迷果芹[Sphallerocarpus gracilis (Bess.) K. -Pol.]、西藏凹乳芹(Vicatia thibetica de Boiss.)、葛缕子(Carum carvi L.)、松潘棱子芹(Pleurospermum franchetianum Hemsl.)、裂叶独活(Heracleum millefolium Diels)、羽苞藁本[Ligusticum daucoides (Franch.) Franch.]、刺果峨参(Anthriscus sylvestris subsp. Nemorosa)及瘤果棱子芹(Pleurospermum wrightianum de Boiss.)。具体样品信息见表1。另从GenBank的Nucleotide数据库下载相关样品序列用于分析比较,信息见表2。
2 方法
收起
2.1 DNA提取
用体积分数75%乙醇擦拭样品表面,自然晾干后,用球磨仪研磨成粉末,称取粉末约30 mg,按植物基因组DNA提取试剂盒说明书进行操作,提取DNA,-20 ℃保存备用。
2.2 PCR扩增及测序
选取通用引物ITS2F(5'-ATGCGATA-CTTGGTGTGAAT-3')和ITS3R(5'-GACGCTTCT-CCAGACTACAAT-3')对样品DNA进行扩增。反应体系为50 μL,包含2×Taq Master Mix缓冲液25 μL、上下游引物各2 μL、DNA模板5 μL,加灭菌超纯水至50 μL。阴性对照为无模板DNA的反应体系。以10 000 r·min-1离心(离心半径为3 cm)5 s后,置于PCR仪中。反应程序为先95 ℃预变性4 min;然后35个循环:94 ℃变性30 s、56 ℃退火30 s、72 ℃延伸45 s;最后72 ℃延伸5 min。PCR产物使用琼脂糖凝胶(质量分数1.5%)电泳来进行检测,确定成功率以及目的条带片段大小,扩增产物委托生工生物工程(上海)公司双向测序。
2.3 数据处理
应用CodonCode Aligner V6.0对测序所获得的数据进行校对拼接,去除引物及低质量区,基于隐马尔科夫模型(HMMer)的注释方法去除5.8 S和28 S区段,即可获得ITS2序列信息。
采用基于局部序列比对算法的搜索工具(BLAST),在美国国家生物技术信息中心(NCBI, https://blast.ncbi.nlm.nih.gov/Blast.Cgi)和中药材DNA条形码鉴定系统(http://barcode.ndctcm.org/china/),应用相似性搜索法[8]对实验样品的不同单倍型序列进行BLAST鉴定分析。
采用MEGA6.0进行比对,对序列的变异位点进行分析,不同物种的单倍型为A1、B1、C1,同一物种的不同单倍型为A1、A2、A3;基于K2-P模型计算样品种内以及种间的遗传距离,使用邻接法构建聚类树[8];登录二级结构预测网站 http://its2. bioapps. biozentrum. uni-wuerzburg. de/,对不同物种的主导单倍型进行ITS2二级结构的预测。
3 结果与分析
收起
3.1 DNA 模板质量检测
对所提取的样品DNA进行PCR扩增检测,通用引物ITS2F和ITS3R能够扩增成功,片段长度为450 bp左右,实验样品皆有亮带扩增出来,见图1。
3.2 BLAST鉴定结果分析
基于NCBI和中药材DNA条形码鉴定系统的BLAST鉴定结果与性状鉴定结果一致。
3.3 序列信息分析
通过MEGA 6.0对序列信息进行了分析,具体见表3。
3.4 变异位点分析
对不同来源的加哇及其易混品ITS2序列变异位点进行统计分析,结果见表4。
3.5 K2-P遗传距离分析
加哇及其易混品的遗传距离见表5。种内最大遗传距离0~0.033,除松潘棱子芹与瘤果棱子芹外(两者种间距离0.024~0.034),种间最小遗传距离0.144~0.462,远大于种内最大遗传距离,具有明显的条形码间距,说明K2-P遗传距离可很好地区分开此9种,但无法完全区分松潘棱子芹与瘤果棱子芹。结果见表5。
3.6 N-J树聚类分析
构建加哇及其易混品的ITS2序列N-J树,设置bootstrap 1 000次重复,支上数值仅显示自展支持率≥50%,见图2。可看出,各物种均能以>50%的自展支持率各自聚为1支,单系性良好。
3.7 ITS2二级结构分析
进行ITS2二级结构预测时,数据库有与西藏凹乳芹、松潘棱子芹、裂叶独活、羽苞藁本、瘤果棱子芹主导单倍型相同,或者高度相似的模型;西藏棱子芹、迷果芹、刺果峨参、葛缕子主导单倍型可以直接生成二级结构,见图3。可见,加哇与其易混品在4个螺旋区的茎环数目、大小、位置有所差异,依据ITS2二级结构可以直观地进行区分,具体分析如下。
可以看出所有物种的二级结构都是典型的1环4臂结构:一个中心环和4个螺旋区,每个螺旋上又有茎环结构,其中螺旋Ⅲ最长。通过茎环数目(表6)可区分西藏棱子芹、葛缕子、裂叶独活、羽苞藁本、瘤果棱子芹,无法区分迷果芹与刺果峨参、西藏凹乳芹与松潘棱子芹,但可以通过茎环的大小及位置对四者进行区分。
迷果芹与刺果峨参在螺旋Ⅰ中均具有四环,但迷果芹第二环大于其余三环且4个环分布较为均匀,刺果峨参4个环大小相似,且分布较为集中;在螺旋Ⅲ中均具有五环,但迷果芹第一环小于其余四环,刺果峨参第一环大于其余四环,故能区分两者。
西藏凹乳芹与松潘棱子芹在螺旋Ⅰ中均具有三环,但西藏凹乳芹第二环与第三环分布集中,而松潘棱子芹三个环分布较为均匀;在螺旋Ⅱ中均具有两环,但西藏凹乳芹第一环与第二环大小较为接近,而松潘棱子芹第一环明显小于第二环,故能区分两者。
4 讨论
收起
加哇及其混淆品从药材上难以通过传统的鉴别方法将其完全区分,故使用2020年版《中国药典》四部通则[13]收载的中药材DNA条形码分子鉴定法。其不受个体形态和完整性的影响,方法易于统一、标准化,是传统鉴定方法的有效补充[14]。本研究基于前期的基原鉴定,提取72批加哇及其易混品的DNA,获取ITS2序列信息,并从GenBank数据库下载30条序列,对所有102条序列进行比对分析,发现通过K2-P遗传距离分析可显著区分除松潘棱子芹与瘤果棱子芹外的所有基原,无法区分的松潘棱子芹与瘤果棱子芹可通过N-J树聚类分析及ITS2二级结构分析得到区分。此方法利用ITS2一级核酸序列分析,并结合了ITS2二级结构实现对其物种的鉴定[8],可以作为DNA条形码鉴别中药材的辅助手段。此方法可用于加哇及其混淆品基原种的鉴别,为保障加哇的临床用药安全和疗效提供了参考依据,对其资源的开发利用也有着重要的价值。
通过K2-P遗传距离分析发现,松潘棱子芹与瘤果棱子芹之间不具有明显的条形码间距,且在聚类分析中,两支也以100%的置信度聚为一大支,可以看出,两者的亲缘关系较为接近,这与植物分类学的观点是一致的:在《中国植物志》中,两者均为伞形科棱子芹属植物,从分子生物学的角度验证了其归属的可信度[10]。
基金
收起
  • 国家药品监督管理局药品监管科学体系建设重点项目资助(RS2024Z006)
文献
收起
参考文献 引证文献
排序方式：
[1]
Yutuo·Yuandangongbu. Four Medical Classics(四部医典)[M]. Shanghai: Shanghai Scientific & Technical Publishers,1987:169.
[2]
Pharmaceutical Standards of the Ministry of Health of the People's Republic of China: Tibetan Medicine Volume 1(中华人民共和国卫生部药品标准:藏药第一册)[S]. 1995:340.
[3]
Sichuan Provincial Standard for Traditional Chinese Medicine(四川省中药材标准)[S]. 2011:284-287.
[4]
Volume Ⅰ of the Standards for Tibetan Medicinal Materials in the Tibet Autonomous Region(西藏自治区藏药材标准第一册)[S]. 2012:54.
[5]
Qinghai Provincial Standard for Tibetan Medicinal Materials(青海省藏药材标准)[S]. 2020:107-113.
[6]
TIAN R, WU Y, GU W, et al. Molecular identification of Erythrinae Cortex and its adulterants based on ITS2 sequence[J]. Chin Tradit Herb Drugs(中草药), 2021, 52(1):211-218.
[7]
HEBERT P D N, CYWINSKA A, BALL S L, et al. Biological identifications through DNA barcodes[J]. Proc R Soc Lond B, 2003, 270(1512):313-321.
[8]
GAO B X, QI J L, ZHONG H, et al. Identification of commonly used origins of Tibetan medicine Lagotidis Herba based on ITS2 barcode and market analysis[J]. Chin Tradit Herb Drugs(中草药), 2022, 53(21):6857-6864.
[9]
QI J L, GAO B X, GOU Y, et al. Identification of zanthoxylum armatum and its closely related species based on ITS2 sequence[J]. Mod Chin Med(中国现代中药), 2021, 23(5):786-791,814.
[10]
SUN S Y, CHEN Y, WANG Q R, et al. Molecular Identification of Lysimachia christinae and its Substitute andAdulterants based on the ITS2 and rbcL Barcodes[J]. J Chin Med Mater(中药材), 2020, 43(6):1336-1342.
[11]
LIU Q, GU W, YANG B, et al. Molecular identification of Cynanchum auriculatum and its closely related speciesbased on ITS2 sequence[J]. J Chin Med Mater(中药材), 2018, 49(24):5901-5909.
[12]
CHENG B, DILINUER T, YU L T, et al. Identification of uighur medicine ribes nigrum and its adulterants based onInternal transcribed spacer 2[J]. Chin Pharm J(中国药学杂志), 2023, 58(3):231-235.
[13]
Ch.P (2020) VolⅣ(中国药典2020年版.四部) [S]. 2020:126.
[14]
CHEN S L. Standard DNA Barcodes of Chinese Materia Medica in Chinese Pharmacopoeia(中国药典中药材DNA条形码标准序列)[M]. Beijing: Science Press, 2015: 23.
2024年第59卷第8期
PDF下载
34
15
引用本文
BibTeX
文章信息
  • 接收时间：2023-02-21
  • 首发时间：2026-04-08
  • 出版时间：2024-04-22
补充材料
相关文章
文章信息
作者
出版历史
  • 收稿日期：2023-02-21
基金
国家药品监督管理局药品监管科学体系建设重点项目资助(RS2024Z006)
作者信息
    1 四川省药品检验研究院, 国家药品监督管理局中成药质量评价重点实验室, 成都 611731
    2 泸州医疗器械职业学院, 四川 泸州 646000
    3 成都中医药大学药学院, 西南特色中药资源国家重点实验室,中药材标准化教育部重点实验室, 四川 成都 611137

通讯作者:

*高必兴,男,主管中药师 研究方向:中药及民族药质量研究 Tel:(028)87877143;
连艳,女,高级实验师研究方向:中药品种、品质与资源研究 Tel:(028)61800231
参考文献
分享链接
https://castjournals.cast.org.cn/joweb/zgyxzz/CN/1248601952666412025
分享至
全文二维码

扫描看全文

引用本文
BibTeX
本文的引用情况
2种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
关闭全屏
相关链接
论文
最新文章
热读文章
热点专题
内参
蓝皮书
产业发展报告
传播力报告
期刊分级目录
资讯
学术新闻
行业新闻
学术会议
行业会议
关于
关于我们
联系我们
北京市海淀区学院南路86号
100081
010-62199257
qkjq@cast.org.cn

中国科学技术协会版权所有 京ICP 备10216604号-4 海淀分局备案 1101084647
科技导报社主办 中国科协信息中心技术支持