Article(id=1248601551699333610, tenantId=1146029695717560320, journalId=1190317699101192196, issueId=1248601546695528820, articleNumber=1001-2494(2024)07-0579-10, orderNo=null, doi=10.11669/cpj.2024.07.003, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=research-article, receivedDate=1676563200000, receivedDateStr=2023-02-17, revisedDate=null, revisedDateStr=null, acceptedDate=null, acceptedDateStr=null, onlineDate=1775619407820, onlineDateStr=2026-04-08, pubDate=1712505600000, pubDateStr=2024-04-08, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1775619407820, onlineIssueDateStr=2026-04-08, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1775619407820, creator=13701087609, updateTime=1775619407820, updator=13701087609, issue=Issue{id=1248601546695528820, tenantId=1146029695717560320, journalId=1190317699101192196, year='2024', volume='59', issue='7', pageStart='561', pageEnd='656', issueExtLink='null', onlineDate='null', pubDate='null', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1775619406627, creator=13701087609, updateTime=1775619979013, updator=13701087609, preIssue=null, nextIssue=null, ext={EN=IssueExt(id=1248603947515142525, tenantId=1146029695717560320, journalId=1190317699101192196, issueId=1248601546695528820, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1248603947515142526, tenantId=1146029695717560320, journalId=1190317699101192196, issueId=1248601546695528820, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null}, startPage=579, endPage=588, ext={EN=ArticleExt(id=1248601551980351996, articleId=1248601551699333610, tenantId=1146029695717560320, journalId=1190317699101192196, language=EN, title=Effects of Carbohydrate Components of Fungus S7 on Seed Germination of
Dendrobium officinale, columnId=null, journalTitle=Chinese Pharmaceutical Journal, columnName=null, runingTitle=null, highlight=null, articleAbstract=
OBJECTIVE To investigate the effects of carbohydrate components of fungus S7 (Tulasnella sp.) on seed germination of Dendrobium officinale. METHODS The polysaccharides of S7 mycelia (MP) and of S7 fermentation broth (FP) were prepared by water extraction and alcohol precipitation. MP and FP were hydrolyzed by trifluoroacetic acid to obtain hydrolysates, MPH and FPH. The monosaccharide composition of MP and FP were determined by PMP-HPLC. MP, FP, MPH, FPH, and the unique and mixed monosaccharide of MP were added to water agar (WA) and 1/2 MS media respectively to study the effects of carbohydrate on the seed germination of Dendrobium officinale. RESULTS The germination rate of WA medium group (WACK1) was (88.65±4.71)%, and seeds germinated to stage 3 (protocorm). Compared with WACK1, adding MP and MPH had no significant effect on germination rate (P>0.05), and seeds could germinate to stage 4 (having one leaf), while adding FPH could significantly reduce germination rate (P<0.05). MP was composed of glucose, galactose, glucuronic acid, xylose, mannose, fucose, ribose and arabinose in a molar ratio of 15.05∶1.00∶0.70∶0.35∶0.30∶0.29∶0.13∶0.13. FP was composed of arabinose, galactose, xylose and glucuronic acid in a molar ration of 1.22∶1.00∶0.71∶0.47. Fucose was the unique monosaccharide component of MP. The germination rate and stage 5 (having two leaves) germination rate of 1/2 MS medium group(MSCK) was (98.10±0.46)% and (50.97±4.33)%, respectively. Compared with MSCK, adding 0.20-20 mg·L-1 fucose had no significant effect on germination rate (P>0.05), while significantly increased stage 5 germination rate (P<0.05), which was 1.36-1.47 times that of MSCK. CONCLUSION MP and MPH promote the development of protocorm of D.officinale. Fucose is an active component in MPH that could promote the further differentiation and development of seeds after germination.
, correspAuthors=Xu ZENG, Shunxing GUO, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, authorCompany=null, fund=null, authors=null, authorsList=Tongyao CHEN, Xiaomei CHEN, Jianwen YANG, Yuanyuan LI, Xiandan QIU, Airong WANG, Xu ZENG, Shunxing GUO), CN=ArticleExt(id=1248601553490301507, articleId=1248601551699333610, tenantId=1146029695717560320, journalId=1190317699101192196, language=CN, title=胶膜菌属真菌S7糖类成分对铁皮石斛种子萌发的影响, columnId=1190352405612040510, journalTitle=中国药学杂志, columnName=论著, runingTitle=null, highlight=null, articleAbstract=
目的 研究胶膜菌属真菌S7(Tulasnella sp.)糖类成分对铁皮石斛种子萌发的影响。方法 通过水提醇沉法制备S7菌丝多糖和发酵液多糖。三氟乙酸水解制备菌丝多糖和发酵液多糖的水解物。利用柱前衍生高效液相色谱法(PMP-HPLC)测定多糖的单糖组成。在水琼脂和1/2 MS培养基中分别添加菌丝多糖及其水解物,发酵液多糖及其水解物,以及菌丝多糖特有单糖组分和混合单糖组分,研究S7糖类成分对铁皮石斛种子萌发的影响。结果 水琼脂培养基对照组(WACK1)萌发率(88.65±4.71)%,种子能萌发至3级(原球茎)。与WACK1相比,添加菌丝多糖及其水解物对萌发率没有显著影响(P>0.05),但种子能萌发至4级(1片叶);添加发酵液多糖水解物能显著降低萌发率(P<0.05)。菌丝多糖由葡萄糖、半乳糖、葡萄糖醛酸、木糖、甘露糖、岩藻糖、核糖和阿拉伯糖组成,摩尔比为15.05∶1.00∶0.70∶0.35∶0.30∶0.29∶0.13∶0.13。发酵液多糖由阿拉伯糖、半乳糖、木糖和葡萄糖醛酸组成,摩尔比为1.22∶1.00∶0.71∶0.47。岩藻糖是菌丝多糖特有的单糖组分。1/2 MS培养基对照组(MSCK)萌发率和5级萌发率(2片叶)分别为(98.10±0.46)%和(50.97±4.33)%。与MSCK相比,添加0.20~20 mg·L-1岩藻糖对萌发率没有显著影响(P>0.05),但5级萌发率有显著提高(P<0.05),是MSCK的1.36~1.47倍。结论 S7菌丝体多糖及其水解物具有促进铁皮石斛原球茎发育的作用,岩藻糖是S7菌丝多糖水解物中促进种子萌发后进一步分化发育的活性成分。
, correspAuthors=曾旭, 郭顺星, authorNote=null, correspAuthorsNote=
*曾旭,男,博士,副研究员 研究方向:药用植物菌根生物学 Tel:(010)57833259;
郭顺星,男,博士,研究员,博士生导师 研究方向:菌根生物学 Tel:(010)57833231
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陈彤垚,女,博士研究生 研究方向:药用植物菌根生物学;
陈晓梅,女,博士,研究员 研究方向:药用植物菌根生物学。
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陈晓梅,女,博士,研究员 研究方向:药用植物菌根生物学。
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2017,
37(6): 763-767., articleTitle=Composition of mycorrhizal fungi and symbiotic relationship of
Cypripedium yunnanense, refAbstract=null)], funds=[Fund(id=1249073377533694138, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601551699333610, awardId=2021-I2M-1-031, language=CN, fundingSource=中国医学科学院医学与健康科技创新工程项目资助(2021-I2M-1-031), fundOrder=null, country=null), Fund(id=1249073377621774524, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601551699333610, awardId=81573526, language=CN, fundingSource=国家自然科学基金项目资助(81573526), fundOrder=null, country=null)], companyList=[AuthorCompany(id=1249073365231801294, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601551699333610, xref=null, ext=[AuthorCompanyExt(id=1249073365240189902, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601551699333610, companyId=1249073365231801294, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=Institute of Medicinal Plant Development, Peking Union Medical College Chinese Academy of Medical Sciences, Key Laboratory of Bioactive Substances and Resource Utilization of Chinese Herbal Medicine, Ministry of Education, Beijing 100193, China), AuthorCompanyExt(id=1249073365248578511, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601551699333610, companyId=1249073365231801294, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=中国医学科学院北京协和医学院药用植物研究所, 教育部中草药物质基础与资源利用重点实验室, 北京 100193)])], figs=[ArticleFig(id=1249073373557493880, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601551699333610, language=EN, label=Fig.1, caption=
HPLC chromatograms of the derivatives of the standards and the hydrolysis product of polysaccharides A-blank solution; B-standards; C-MPH; D-FPH; E-WPH; Peak 1-mannose; Peak 2-glucosamine; Peak 3-ribose; Peak 4-rhamnose; Peak 5-glucuronic acid; Peak 6-galacturonic acid; Peak 7-glucose; Peak 8-galactose; Peak 9-xylose; Peak 10-arabinose; Peak 11-fucose.
, figureFileSmall=LTPK/VDo6v4iwxziU6g8NA==, figureFileBig=uvLxgd5qxKxGd4sBaZhS/Q==, tableContent=null), ArticleFig(id=1249073373624602747, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601551699333610, language=CN, label=图1, caption=
对照品和多糖水解产物衍生化的HPLC色谱图 A-空白对照;B-混合对照品;C-菌丝多糖水解物;D-发酵液多糖水解物;E-麦麸培养基多糖水解物;峰1-甘露糖;峰2-氨基葡萄糖;峰3-核糖;峰4-鼠李糖;峰5-葡萄糖醛酸;峰6-半乳糖醛酸;峰7-葡萄糖;峰8-半乳糖;峰9-木糖;峰10-阿拉伯糖;峰11-岩藻糖。
, figureFileSmall=LTPK/VDo6v4iwxziU6g8NA==, figureFileBig=uvLxgd5qxKxGd4sBaZhS/Q==, tableContent=null), ArticleFig(id=1249073373771403390, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601551699333610, language=EN, label=Fig.2, caption=
Effects of WA medium containing mixed monosaccharides and fucose on protocorm development A-WACK2; B-WALMix; C-WAMMix; D-WAHMix; E-WALFuc; F-WAMFuc; G-WAHFuc; 1)P<0.05, significant difference compared with WACK2 during the same period.
, figureFileSmall=lt0jrOh8akoPCVahrpKFRA==, figureFileBig=qdXqdPh6WoqUYQ83bjtsFA==, tableContent=null), ArticleFig(id=1249073373842706563, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601551699333610, language=CN, label=图2, caption=
WA培养基添加混合单糖和岩藻糖对原球茎发育的影响 A-水琼脂培养基中未添加混合单糖和海藻糖;B-水琼脂培养基中混合单糖浓度32 mg·L-1;C-水琼脂培养基中海藻糖浓度32 mg·L-1;D-水琼脂培养基中混合单糖浓度320 mg·L-1;E-水琼脂培养基中海藻糖浓度320 mg·L-1;F-水琼脂培养基中混合单糖浓度3.2 g·L-1;G-水琼脂培养基中海藻糖浓度3.2 g·L-1;与相同时期的WACK2相比有显著差异, 1)P<0.05。
, figureFileSmall=lt0jrOh8akoPCVahrpKFRA==, figureFileBig=qdXqdPh6WoqUYQ83bjtsFA==, tableContent=null), ArticleFig(id=1249073373934981252, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601551699333610, language=EN, label=Fig.3, caption=
Seed germination for day 90 on WA media containing mixed monosaccharides and fucose A1-A4-under vision; B1-B4-under a stereoscope. Scale bar=0.5 mm. A1 and B1-MSCK; A2 and B2-MSLFuc; A3 and B3-MSMFuc; A4 and B4-MSHFuc.
, figureFileSmall=Rpwpuhf799y9E5xJbddTKA==, figureFileBig=WMUqoO3T30JPxjWTxRwZgQ==, tableContent=null), ArticleFig(id=1249073374002090118, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601551699333610, language=CN, label=图3, caption=
种子在含岩藻糖的WA培养基上培养90 d的萌发情况 A1~A4-肉眼观察;B1~B4-体视镜观察,标尺=0.5 mm。A1和B1-WACK2;A2和B2-WALFuc;A3和B3-WAMFuc;A4和B4-WAHFuc。
, figureFileSmall=Rpwpuhf799y9E5xJbddTKA==, figureFileBig=WMUqoO3T30JPxjWTxRwZgQ==, tableContent=null), ArticleFig(id=1249073374069198986, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601551699333610, language=EN, label=Fig.4, caption=
Effects of mixed monosaccharides and fucose in 1/2 MS medium on development of protocorm A-MSCK; B-MSLMix; C-MSMMix; D-MSHMix; E-MSLFuc; F-MSMFuc; G-MSHFuc;1)P<0.05, significant difference compared with MSCK during the same period.
, figureFileSmall=Zvcj6U6RxjA9MetR7VYswg==, figureFileBig=mp0CTyjw8l669H2XJyTd3A==, tableContent=null), ArticleFig(id=1249073374144696461, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601551699333610, language=CN, label=图4, caption=
1/2 MS培养基添加混合单糖和岩藻糖对原球茎发育的影响 A-1/2MS培养基中未添加混合单糖和海藻糖;B-1/2MS培养基中混合单糖浓度32 mg·L-1;C-1/2MS培养基中海藻糖浓度32 mg·L-1;D-1/2MS培养基中混合单糖浓度320 mg·L-1;E-1/2MS培养基中海藻糖浓度320 mg·L-1;F-1/2MS培养基中混合单糖浓度3.2 g·L-1;G-1/2MS培养基中海藻糖浓度3.2 g·L-1;与相同时期的MSCK相比有显著差异,1)P<0.05。
, figureFileSmall=Zvcj6U6RxjA9MetR7VYswg==, figureFileBig=mp0CTyjw8l669H2XJyTd3A==, tableContent=null), ArticleFig(id=1249073374283108496, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601551699333610, language=EN, label=Fig.5, caption=
Seed germination for day 90 on 1/2 MS media containing mixed monosaccharides and fucose A1-A4-under vision; B1-B4-under a stereoscope, scale bar=0.5 mm. A1 and B1-MSCK; A2 and B2-MSLFuc; A3 and B3-MSMFuc; A4 and B4-MSHFuc.
, figureFileSmall=0Co+NMooWQwunuELBczbQg==, figureFileBig=lKq3lDTRY2r1VqjmU0z2XA==, tableContent=null), ArticleFig(id=1249073374371188883, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601551699333610, language=CN, label=图5, caption=
种子在含岩藻糖的1/2 MS培养基上培养90 d的萌发情况 A1~A4-肉眼观察;B1~B4-体视镜观察,标尺=0.5 mm。A1和B1-MSCK;A2和B2-MSLFuc;A3和B3-MSMFuc;A4和B4-MSHFuc。
, figureFileSmall=0Co+NMooWQwunuELBczbQg==, figureFileBig=lKq3lDTRY2r1VqjmU0z2XA==, tableContent=null), ArticleFig(id=1249073374467657875, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601551699333610, language=EN, label=Tab.1, caption=
Mobile phase and gradient elution for monosaccharide composition analysis
, figureFileSmall=null, figureFileBig=null, tableContent=
| tR/min | Mobile phase A/% | Mobile phase B/% |
| 0.00 | 83.0 | 17.0 |
| 30.00 | 84.0 | 16.0 |
| 30.01 | 87.0 | 13.0 |
| 38.00 | 87.0 | 13.0 |
| 40.00 | 84.0 | 16.0 |
| 85.00 | 84.0 | 16.0 |
| 85.01 | 83.0 | 17.0 |
| 90.00 | 83.0 | 17.0 |
), ArticleFig(id=1249073374568321177, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601551699333610, language=CN, label=表1, caption=
单糖组成测定的流动相梯度洗脱条件
, figureFileSmall=null, figureFileBig=null, tableContent=
| tR/min | Mobile phase A/% | Mobile phase B/% |
| 0.00 | 83.0 | 17.0 |
| 30.00 | 84.0 | 16.0 |
| 30.01 | 87.0 | 13.0 |
| 38.00 | 87.0 | 13.0 |
| 40.00 | 84.0 | 16.0 |
| 85.00 | 84.0 | 16.0 |
| 85.01 | 83.0 | 17.0 |
| 90.00 | 83.0 | 17.0 |
), ArticleFig(id=1249073376141185179, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601551699333610, language=EN, label=Tab.2, caption=
Monosaccharide composition and concentrations in D.officinale seed germination medium
, figureFileSmall=null, figureFileBig=null, tableContent=
Monosaccharide
composition | ρ/mg·L-1 |
| LMix | LFuc | MMix | MFuc | HMix | HFuc |
| Mannose(Man) | 0.23 | 0 | 2.3 | 0 | 23 | 0 |
| Ribose(Rib) | 0.08 | 0 | 0.8 | 0 | 8 | 0 |
| Glucuronic acid(GlcA) | 0.57 | 0 | 5.7 | 0 | 57 | 0 |
| Glucose(Glu) | 11.41 | 0 | 114.1 | 0 | 1 141 | 0 |
| Galactose(Gal) | 0.76 | 0 | 7.6 | 0 | 76 | 0 |
| Xylose(Xyl) | 0.22 | 0 | 2.2 | 0 | 22 | 0 |
| Arabinose(Ara) | 0.08 | 0 | 0.8 | 0 | 8 | 0 |
| Fucose(Fuc) | 0.20 | 0.20 | 2.0 | 2.0 | 20 | 20 |
), ArticleFig(id=1249073376304763038, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601551699333610, language=CN, label=表2, caption=
培养基中的单糖组分的添加浓度
, figureFileSmall=null, figureFileBig=null, tableContent=
Monosaccharide
composition | ρ/mg·L-1 |
| LMix | LFuc | MMix | MFuc | HMix | HFuc |
| Mannose(Man) | 0.23 | 0 | 2.3 | 0 | 23 | 0 |
| Ribose(Rib) | 0.08 | 0 | 0.8 | 0 | 8 | 0 |
| Glucuronic acid(GlcA) | 0.57 | 0 | 5.7 | 0 | 57 | 0 |
| Glucose(Glu) | 11.41 | 0 | 114.1 | 0 | 1 141 | 0 |
| Galactose(Gal) | 0.76 | 0 | 7.6 | 0 | 76 | 0 |
| Xylose(Xyl) | 0.22 | 0 | 2.2 | 0 | 22 | 0 |
| Arabinose(Ara) | 0.08 | 0 | 0.8 | 0 | 8 | 0 |
| Fucose(Fuc) | 0.20 | 0.20 | 2.0 | 2.0 | 20 | 20 |
), ArticleFig(id=1249073376422203552, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601551699333610, language=EN, label=Tab.3, caption=
Calibration data of 11 monosaccharides of the S7 mycelia and fermentation broth polysaccharides
, figureFileSmall=null, figureFileBig=null, tableContent=
| Monosaccharide | Regression
equation | Linear range
/μg·mL-1 | r2 |
| Mannose | Y=1 458.2X-23 647 | 100.30-1 823.64 | 0.999 2 |
| Glucosamine | Y=1 001.1X-7 391.3 | 100.45-1 826.36 | 0.999 6 |
| Ribose | Y=1 543.6X | 100.25-1 822.73 | 0.999 1 |
| Rhamnose | Y=1 206.8X-32 101 | 99.50-1 809.09 | 0.999 1 |
| Glucuronic acid | Y=1 114.6X-86 524 | 99.70-1 812.73 | 0.999 9 |
| Galacturonic acid | Y=1 009.7X-34 383 | 99.65-1 811.82 | 0.999 5 |
| Glucose | Y=1 207.6X-98 685 | 100.55-1 828.18 | 0.999 9 |
| Galactose | Y=1 219.8X-84 753 | 100.25-1 822.73 | 0.999 6 |
| Xylose | Y=1 377.3X-76 913 | 100.35-1 824.55 | 0.999 9 |
| Arabinose | Y=1 457.2X-67 095 | 99.45-1 808.18 | 0.999 9 |
| Fucose | Y=1 130.5X-76 290 | 100.00-1 818.18 | 0.999 2 |
), ArticleFig(id=1249073376527061155, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601551699333610, language=CN, label=表3, caption=
菌丝体及发酵液多糖中单糖对照品的标准曲线、相关系数和线性范围
, figureFileSmall=null, figureFileBig=null, tableContent=
| Monosaccharide | Regression
equation | Linear range
/μg·mL-1 | r2 |
| Mannose | Y=1 458.2X-23 647 | 100.30-1 823.64 | 0.999 2 |
| Glucosamine | Y=1 001.1X-7 391.3 | 100.45-1 826.36 | 0.999 6 |
| Ribose | Y=1 543.6X | 100.25-1 822.73 | 0.999 1 |
| Rhamnose | Y=1 206.8X-32 101 | 99.50-1 809.09 | 0.999 1 |
| Glucuronic acid | Y=1 114.6X-86 524 | 99.70-1 812.73 | 0.999 9 |
| Galacturonic acid | Y=1 009.7X-34 383 | 99.65-1 811.82 | 0.999 5 |
| Glucose | Y=1 207.6X-98 685 | 100.55-1 828.18 | 0.999 9 |
| Galactose | Y=1 219.8X-84 753 | 100.25-1 822.73 | 0.999 6 |
| Xylose | Y=1 377.3X-76 913 | 100.35-1 824.55 | 0.999 9 |
| Arabinose | Y=1 457.2X-67 095 | 99.45-1 808.18 | 0.999 9 |
| Fucose | Y=1 130.5X-76 290 | 100.00-1 818.18 | 0.999 2 |
), ArticleFig(id=1249073376703221926, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601551699333610, language=EN, label=Tab.4, caption=
Monosaccharide composition analysis of S7 mycelia and fermentation broth the polysaccharides. n=3,$\stackrel{-}{x}$±s
, figureFileSmall=null, figureFileBig=null, tableContent=
Monosaccharide
composition | Polysaccharides |
| S7 Mycelia | S7 fermentation broth | Wheat Bran Agar |
| Content/% | Molar ratio | Content/% | Molar ratio | Content/% | Molar ratio |
| Mannose | 0.72±0.01 | 0.30 | 0 | 0 | 0 | 0 |
| Ribose | 0.26±0.00 | 0.13 | 0 | 0 | 0 | 0 |
| Glucuronic acid | 1.78±0.06 | 0.70 | 1.13±0.03 | 0.47 | 0 | 0 |
| Glucose | 35.66±1.22 | 15.05 | 0 | 0 | 5.30±0.05 | 5.38 |
| Galactose | 2.37±0.03 | 1.00 | 2.21±0.06 | 1.00 | 0.98±0.08 | 1.00 |
| Xylose | 0.69±0.02 | 0.35 | 1.32±0.04 | 0.71 | 3.21±0.25 | 3.92 |
| Arabinose | 0.26±0.01 | 0.13 | 2.26±0.08 | 1.22 | 2.17±0.08 | 2.64 |
| Fucose | 0.62±0.02 | 0.29 | 0 | 0 | 0 | 0 |
), ArticleFig(id=1249073376803885226, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601551699333610, language=CN, label=表4, caption=
菌丝体及发酵液中多糖的单糖组成分析. n=3,$\stackrel{-}{x}$±s
, figureFileSmall=null, figureFileBig=null, tableContent=
Monosaccharide
composition | Polysaccharides |
| S7 Mycelia | S7 fermentation broth | Wheat Bran Agar |
| Content/% | Molar ratio | Content/% | Molar ratio | Content/% | Molar ratio |
| Mannose | 0.72±0.01 | 0.30 | 0 | 0 | 0 | 0 |
| Ribose | 0.26±0.00 | 0.13 | 0 | 0 | 0 | 0 |
| Glucuronic acid | 1.78±0.06 | 0.70 | 1.13±0.03 | 0.47 | 0 | 0 |
| Glucose | 35.66±1.22 | 15.05 | 0 | 0 | 5.30±0.05 | 5.38 |
| Galactose | 2.37±0.03 | 1.00 | 2.21±0.06 | 1.00 | 0.98±0.08 | 1.00 |
| Xylose | 0.69±0.02 | 0.35 | 1.32±0.04 | 0.71 | 3.21±0.25 | 3.92 |
| Arabinose | 0.26±0.01 | 0.13 | 2.26±0.08 | 1.22 | 2.17±0.08 | 2.64 |
| Fucose | 0.62±0.02 | 0.29 | 0 | 0 | 0 | 0 |
), ArticleFig(id=1249073376967463083, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601551699333610, language=EN, label=Tab.5, caption=
Germination rates of seeds cultured on WA medium containing mixed monosaccharides and fucose.%,n=6,$\stackrel{-}{x}$±s
, figureFileSmall=null, figureFileBig=null, tableContent=
| t/d | WACK2 | WALMix | WAMMix | WAHMix | WALFuc | WAMFuc | WAHFuc |
| 10 | 1.58±1.56 | 3.30±0.98 | 8.22±5.06 | 2.59±0.57 | 3.84±0.45 | 2.13±0.93 | 2.44±0.98 |
| 20 | 25.68±1.81 | 40.28±1.101) | 35.34±4.081) | 22.22±2.27 | 38.81±4.561) | 41.01±0.991) | 27.54±0.58 |
| 30 | 83.07±5.84 | 91.60±4.87 | 88.37±5.53 | 72.12±6.66 | 87.38±2.79 | 87.53±1.71 | 86.01±4.89 |
| 40 | 91.12±0.88 | 92.13±3.54 | 93.56±4.33 | 87.00±1.65 | 94.47±1.171) | 92.97±4.95 | 93.5±3.01 |
| 50 | 91.15±1.96 | 91.11±2.38 | 92.52±2.79 | 87.59±3.09 | 93.45±2.99 | 91.76±2.09 | 92.63±4.24 |
| 60 | 91.73±1.92 | 92.61±1.98 | 94.60±2.38 | 86.62±1.091) | 90.49±3.17 | 92.46±0.84 | 92.98±1.99 |
| 70 | 91.98±1.51 | 93.15±0.49 | 93.64±1.37 | 86.71±2.521) | 93.55±1.72 | 93.23±1.06 | 93.01±1.90 |
| 80 | 92.41±0.95 | 93.32±2.32 | 94.90±2.11 | 86.70±1.201) | 91.59±4.41 | 92.61±3.76 | 93.34±3.99 |
| 90 | 92.40±4.50 | 95.68±1.37 | 94.94±0.30 | 85.57±1.381) | 95.63±0.12 | 95.18±1.82 | 92.44±0.69 |
), ArticleFig(id=1249073377059737774, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601551699333610, language=CN, label=表5, caption=
种子在含有混合单糖和岩藻糖的水琼脂(WA)培养基上的萌发率.%,n=6,$\stackrel{-}{x}$±s
, figureFileSmall=null, figureFileBig=null, tableContent=
| t/d | WACK2 | WALMix | WAMMix | WAHMix | WALFuc | WAMFuc | WAHFuc |
| 10 | 1.58±1.56 | 3.30±0.98 | 8.22±5.06 | 2.59±0.57 | 3.84±0.45 | 2.13±0.93 | 2.44±0.98 |
| 20 | 25.68±1.81 | 40.28±1.101) | 35.34±4.081) | 22.22±2.27 | 38.81±4.561) | 41.01±0.991) | 27.54±0.58 |
| 30 | 83.07±5.84 | 91.60±4.87 | 88.37±5.53 | 72.12±6.66 | 87.38±2.79 | 87.53±1.71 | 86.01±4.89 |
| 40 | 91.12±0.88 | 92.13±3.54 | 93.56±4.33 | 87.00±1.65 | 94.47±1.171) | 92.97±4.95 | 93.5±3.01 |
| 50 | 91.15±1.96 | 91.11±2.38 | 92.52±2.79 | 87.59±3.09 | 93.45±2.99 | 91.76±2.09 | 92.63±4.24 |
| 60 | 91.73±1.92 | 92.61±1.98 | 94.60±2.38 | 86.62±1.091) | 90.49±3.17 | 92.46±0.84 | 92.98±1.99 |
| 70 | 91.98±1.51 | 93.15±0.49 | 93.64±1.37 | 86.71±2.521) | 93.55±1.72 | 93.23±1.06 | 93.01±1.90 |
| 80 | 92.41±0.95 | 93.32±2.32 | 94.90±2.11 | 86.70±1.201) | 91.59±4.41 | 92.61±3.76 | 93.34±3.99 |
| 90 | 92.40±4.50 | 95.68±1.37 | 94.94±0.30 | 85.57±1.381) | 95.63±0.12 | 95.18±1.82 | 92.44±0.69 |
), ArticleFig(id=1249073377185566898, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601551699333610, language=EN, label=Tab.6, caption=
Germination rates of seeds cultured on 1/2 MS medium containing mixed monosaccharides and fucose.%,n=6,$\stackrel{-}{x}$±s
, figureFileSmall=null, figureFileBig=null, tableContent=
| t/d | MSCK | MSLMix | MSMMix | MSHMix | MSLFuc | MSMFuc | MSHFuc |
| 10 | 43.86±2.79 | 55.18±7.38 | 56.65±6.911) | 52.07±2.721) | 49.26±5.79 | 37.57±0.99 | 47.68±1.94 |
| 20 | 96.11±1.51 | 97.09±2.65 | 96.18±2.75 | 89.17±2.191) | 95.83±2.06 | 95.71±0.90 | 95.04±0.29 |
| 30 | 96.72±1.94 | 97.32±1.44 | 95.07±0.76 | 96.89±1.22 | 97.10±0.89 | 97.31±2.22 | 95.97±0.87 |
| 40 | 96.76±1.17 | 94.58±2.77 | 94.74±2.99 | 98.05±0.11 | 96.06±2.91 | 98.38±1.48 | 96.29±1.08 |
| 50 | 98.47±0.98 | 97.43±0.87 | 96.07±4.15 | 98.46±1.56 | 98.10±1.32 | 99.08±0.93 | 97.51±1.15 |
| 60 | 97.85±0.63 | 97.81±0.95 | 98.65±0.48 | 97.24±1.07 | 97.93±0.68 | 99.14±0.09 | 98.01±0.53 |
| 70 | 98.25±0.77 | 97.23±1.01 | 98.32±0.11 | 97.50±1.10 | 99.24±0.04 | 99.06±0.02 | 98.41±1.19 |
| 80 | 96.93±0.67 | 98.96±1.12 | 97.91±0.35 | 97.87±2.32 | 97.68±1.95 | 97.64±1.12 | 98.57±1.02 |
| 90 | 98.10±0.46 | 99.16±0.06 | 99.05±0.42 | 98.21±1.00 | 98.75±0.58 | 98.93±0.47 | 98.42±1.18 |
), ArticleFig(id=1249073377265258677, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601551699333610, language=CN, label=表6, caption=
种子在含有混合单糖和岩藻糖的1/2 MS培养基上的萌发率.%,n=6,$\stackrel{-}{x}$±s
, figureFileSmall=null, figureFileBig=null, tableContent=
| t/d | MSCK | MSLMix | MSMMix | MSHMix | MSLFuc | MSMFuc | MSHFuc |
| 10 | 43.86±2.79 | 55.18±7.38 | 56.65±6.911) | 52.07±2.721) | 49.26±5.79 | 37.57±0.99 | 47.68±1.94 |
| 20 | 96.11±1.51 | 97.09±2.65 | 96.18±2.75 | 89.17±2.191) | 95.83±2.06 | 95.71±0.90 | 95.04±0.29 |
| 30 | 96.72±1.94 | 97.32±1.44 | 95.07±0.76 | 96.89±1.22 | 97.10±0.89 | 97.31±2.22 | 95.97±0.87 |
| 40 | 96.76±1.17 | 94.58±2.77 | 94.74±2.99 | 98.05±0.11 | 96.06±2.91 | 98.38±1.48 | 96.29±1.08 |
| 50 | 98.47±0.98 | 97.43±0.87 | 96.07±4.15 | 98.46±1.56 | 98.10±1.32 | 99.08±0.93 | 97.51±1.15 |
| 60 | 97.85±0.63 | 97.81±0.95 | 98.65±0.48 | 97.24±1.07 | 97.93±0.68 | 99.14±0.09 | 98.01±0.53 |
| 70 | 98.25±0.77 | 97.23±1.01 | 98.32±0.11 | 97.50±1.10 | 99.24±0.04 | 99.06±0.02 | 98.41±1.19 |
| 80 | 96.93±0.67 | 98.96±1.12 | 97.91±0.35 | 97.87±2.32 | 97.68±1.95 | 97.64±1.12 | 98.57±1.02 |
| 90 | 98.10±0.46 | 99.16±0.06 | 99.05±0.42 | 98.21±1.00 | 98.75±0.58 | 98.93±0.47 | 98.42±1.18 |
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