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Article(id=1248601467309937477, tenantId=1146029695717560320, journalId=1190317699101192196, issueId=1248601466106172220, articleNumber=1001-2494(2024)06-0491-10, orderNo=null, doi=null, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=research-article, receivedDate=1677859200000, receivedDateStr=2023-03-04, revisedDate=null, revisedDateStr=null, acceptedDate=null, acceptedDateStr=null, onlineDate=1775619387700, onlineDateStr=2026-04-08, pubDate=1711036800000, pubDateStr=2024-03-22, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1775619387700, onlineIssueDateStr=2026-04-08, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1775619387700, creator=13701087609, updateTime=1775619387700, updator=13701087609, issue=Issue{id=1248601466106172220, tenantId=1146029695717560320, journalId=1190317699101192196, year='2024', volume='59', issue='6', pageStart='469', pageEnd='558', issueExtLink='null', onlineDate='null', pubDate='null', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1775619387412, creator=13701087609, updateTime=1775619937245, updator=13701087609, preIssue=null, nextIssue=null, ext={EN=IssueExt(id=1248603772348420655, tenantId=1146029695717560320, journalId=1190317699101192196, issueId=1248601466106172220, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1248603772348420656, tenantId=1146029695717560320, journalId=1190317699101192196, issueId=1248601466106172220, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null}, startPage=491, endPage=500, ext={EN=ArticleExt(id=1248601467590955847, articleId=1248601467309937477, tenantId=1146029695717560320, journalId=1190317699101192196, language=EN, title=Rapid Analysis of Chemical Components in Tripterospermum chinense (Migo)H. Smith Based on HPLC-LTQ-Orbitrap-MS/MS Technology, columnId=1248601467007947584, journalTitle=Chinese Pharmaceutical Journal, columnName=Original article, runingTitle=null, highlight=null, articleAbstract=

OBJECTIVE An efficient and stable HPLC-LTQ-Orbitrap-MS/MS method was developed to analyze and identify the chemical constituents from Tripterospermum chinense (Migo)H.Smith rapidly. METHODS The chemical components in T.chinense were analyzed by high performance liquid chromatography linear ion trap orbital trap tandem mass spectrometry (HPLC-LTQ-Orbitrap-MS/MS). HPLC-LTQ-Orbitrap-MS/MS was used to analyze the chemical components in T.chinense quickly. The accurate molecular weight, reference substance and multistage fragment ion information of the compounds obtained by HPLC-LTQ-Orbitrap-MS/MS scanning, combined with relevant literature and fragmentation regularity, were used to achieve the accurate qualitative analysis of T. chinense. RESULTS A total of 92 chemical constituents were identified, including flavonoids, terpenoids, organic acids, anthraquinones, saccharide, ester and the others. Among them, compounds 3, 4-di-O-caffeoylquinic acid、(2S, 3S, 4S, 5R, 6S)-6-[2-(3, 4-dihydroxyphenyl)-5-hydroxy-4-oxochromen-7-yl]oxy-3, 4, 5-trihydroxyoxane-2-carboxylic acid、(1S, 4aS, 7S, 7aS)-1-[(2S, 3R, 4S, 5S, 6R)-6-[[(E)-3-(3, 4-dihydroxyphenyl)prop-2-enoyl]oxymethyl]-3, 4, 5-trihydroxyoxan-2-yl]oxy-7-hydroxy-7-methyl-4a, 5, 6, 7a-tetrahydro-1H-cyclopenta[c]pyran-4-carboxylic acid and Glc-Glc-octadecatrienoyl-sn-glycerol were identified for the first time in this plant by GNPS database alignment. CONCLUSION A rapid qualitative analysis method of chemical components in T.chinense is established, which provides a scientific basis for the extraction and isolation of the chemical constituents of T.chinense and its pharmacodynamic material basis, and provides a certain reference significance for its quality evaluation and application development.

, correspAuthors=Linke MA, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, authorCompany=null, fund=null, authors=null, authorsList=Yi ZHANG, Chunmei TAN, Linke MA, Cheng ZHENG, Bilian CHEN), CN=ArticleExt(id=1248601471776871334, articleId=1248601467309937477, tenantId=1146029695717560320, journalId=1190317699101192196, language=CN, title=基于HPLC-LTQ-Orbitrap-MS/MS技术快速分析肺形草中的化学成分, columnId=1196884516783571586, journalTitle=中国药学杂志, columnName=研究论文, runingTitle=null, highlight=null, articleAbstract=

目的 建立高效、稳定的高效液相色谱-线性离子阱串联质谱联用技术(HPLC-LTQ-Orbitrap-MS/MS)方法,对肺形草全草的化学成分快速识别和鉴定。方法 采用HPLC-LTQ-Orbitrap-MS/MS对肺形草中的化学成分进行快速分析。通过HPLC-LTQ-Orbitrap-MS/MS扫描得到的化合物相对分子质量,对照品和多级碎片离子信息,并结合相关文献以及裂解规律,从而实现对肺形草的准确定性分析。结果 共鉴定出92种化学成分,包括黄酮类、萜类、有机酸类、蒽醌类、糖类、酯类等,其中化合物3,4-di-O-caffeoylquinic acid、(2S,3S,4S,5R,6S)-6-[2-(3,4-dihydroxyphenyl)-5-hydroxy-4-oxochromen-7-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid、(1S,4aS,7S,7aS)-1-[(2S,3R,4S,5S,6R)-6-[[(E)-3-(3,4-dihydroxyphenyl)prop-2-enoyl]oxymethyl]-3,4,5-trihydroxyoxan-2-yl]oxy-7-hydroxy-7-methyl-4a,5,6,7a-tetrahydro-1H-cyclopenta[c]pyran-4-carboxylic acid和Glc-Glc-octadecatrienoyl-sn-glycerol经全球天然产物社会分子网络系统(GNPS)数据库比对,为首次在该植物中鉴别得到。结论 建立了对肺形草中化学成分的快速定性分析的方法,为肺形草化学成分的提取分离及其药效物质基础提供科学依据,并为其质量评价和应用开发提供了一定的参考。

, correspAuthors=马临科, authorNote=null, correspAuthorsNote=
*马临科,男,副主任,中药师
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张艺,女,硕士研究生 研究方向:中药药效物质基础研究和质量分析评价,研究方向:中药药效物质基础研究和质量分析评价 Tel:(0571)87180305

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张艺,女,硕士研究生 研究方向:中药药效物质基础研究和质量分析评价,研究方向:中药药效物质基础研究和质量分析评价 Tel:(0571)87180305

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张艺,女,硕士研究生 研究方向:中药药效物质基础研究和质量分析评价,研究方向:中药药效物质基础研究和质量分析评价 Tel:(0571)87180305

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Notes on Gentianaceae From South China[J]. J Trop Subtrop Bot( 热带亚热带植物学报), 1997(2):13-15., articleTitle=Notes on Gentianaceae From South China, refAbstract=null), Reference(id=1248653100924425078, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601467309937477, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[2], rfOrder=1, authorNames=null, journalName=null, refType=null, unstructuredReference=Ch.P(1977)Vol Ⅰ(中国药典1977年版.一部)[S].1977:366., articleTitle=null, refAbstract=null), Reference(id=1248653101079614328, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601467309937477, doi=null, pmid=null, pmcid=null, year=1999, volume=null, issue=null, pageStart=6266, pageEnd=null, url=null, language=null, rfNumber=[3], rfOrder=2, authorNames=SONG L R, journalName=Chin Herbal Med(中华本草), refType=null, unstructuredReference=SONG L R. Chin Herbal Med(中华本草)[M]. 1999:6266-6267., articleTitle=null, refAbstract=null), Reference(id=1248653101138334586, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601467309937477, doi=null, pmid=null, pmcid=null, year=2014, volume=37, issue=11, pageStart=2002, pageEnd=2004, url=null, language=null, rfNumber=[4], rfOrder=3, authorNames=ZUO Y M, LIU D H, ZHANG Z L, journalName=中药材, refType=null, unstructuredReference=ZUO Y M, LIU D H, ZHANG Z L, et al. Study on chemical components of Tripterospermum chinese[J]. J Chin Med Mater( 中药材), 2014, 37(11):2002-2004., articleTitle=Study on chemical components of Tripterospermum chinese, refAbstract=null), Reference(id=1248653101201249149, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601467309937477, doi=null, pmid=null, pmcid=null, year=2012, volume=36, issue=12, pageStart=920, pageEnd=924, url=null, language=null, rfNumber=[5], rfOrder=4, authorNames=ZHANG T, LI B, CHEN L, journalName=军事医学, refType=null, unstructuredReference=ZHANG T, LI B, CHEN L, et al. Chemical constituents of Tripterospermum chinese[J]. Mil Med Sci( 军事医学), 2012, 36(12):920-924., articleTitle=Chemical constituents of Tripterospermum chinese, refAbstract=null), Reference(id=1248653101276746624, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601467309937477, doi=null, pmid=null, pmcid=null, year=2012, volume=47, issue=11, pageStart=1517, pageEnd=1520, url=null, language=null, rfNumber=[6], rfOrder=5, authorNames=ZHANG T, LI B, CHEN L, journalName=药学学报, refType=null, unstructuredReference=ZHANG T, LI B, CHEN L, et al. A novel lactone from Tripterospermum chinense[J]. Acta Pharm Sin( 药学学报), 2012, 47(11):1517-1520., articleTitle=A novel lactone from Tripterospermum chinense, refAbstract=null), Reference(id=1248653101373215621, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601467309937477, doi=null, pmid=null, pmcid=null, year=2022, volume=28, issue=8, pageStart=139, pageEnd=148, url=null, language=null, rfNumber=[7], rfOrder=6, authorNames=LIANG X P, JI S, DU S B, journalName=中国实验方剂学杂志, refType=null, unstructuredReference=LIANG X P, JI S, DU S B, et al. Analysis of chemical constituents in different parts of Gentiana straminea Based on UPLC-Q-TOF-MS/MS[J]. Chin J Exp Tradit Med Form( 中国实验方剂学杂志), 2022, 28(8):139-148., articleTitle=Analysis of chemical constituents in different parts of Gentiana straminea Based on UPLC-Q-TOF-MS/MS, refAbstract=null), Reference(id=1248653101457101703, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601467309937477, doi=null, pmid=null, pmcid=null, year=2016, volume=47, issue=18, pageStart=3175, pageEnd=3180, url=null, language=null, rfNumber=[8], rfOrder=7, authorNames=WANG C S, DONG H J, BAO Y T, journalName=中草药, refType=null, unstructuredReference=WANG C S, DONG H J, BAO Y T, et al. Rapid analysis on chemical constituents from roots of Gentiana crasicaulis by ultra-high performance liquid chromatography coupled with hybrid quadrupole-orbitrap mass spectrometry[J]. Chin Tradit Herb Drugs( 中草药), 2016, 47(18):3175-3180., articleTitle=Rapid analysis on chemical constituents from roots of Gentiana crasicaulis by ultra-high performance liquid chromatography coupled with hybrid quadrupole-orbitrap mass spectrometry, refAbstract=null), Reference(id=1248653101557765002, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601467309937477, doi=null, pmid=null, pmcid=null, year=2018, volume=24, issue=24, pageStart=89, pageEnd=94, url=null, language=null, rfNumber=[9], rfOrder=8, authorNames=LUO J, ZHAO L J, BAO J P, journalName=中国实验方剂学杂志, refType=null, unstructuredReference=LUO J, ZHAO L J, BAO J P, et al. Analysis of chemical compositions in Gentiana rhodantha by UPLC-Q-TOF-MS[J]. Chin J Exp Tradit Med Form( 中国实验方剂学杂志), 2018, 24(24):89-94., articleTitle=Analysis of chemical compositions in Gentiana rhodantha by UPLC-Q-TOF-MS, refAbstract=null), Reference(id=1248653101637456780, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601467309937477, doi=null, pmid=null, pmcid=null, year=2018, volume=49, issue=10, pageStart=2328, pageEnd=2335, url=null, language=null, rfNumber=[10], rfOrder=9, authorNames=CHEN J K, ZENG R, journalName=中草药, refType=null, unstructuredReference=CHEN J K, ZENG R. Application of metabolomics approach to study on chemical constituents in different parts of Gentiana crasicaulis based on UPLC-ESI-HRMSn[J]. Chin Tradit Herb Drugs( 中草药), 2018, 49(10):2328-2335., articleTitle=Application of metabolomics approach to study on chemical constituents in different parts of Gentiana crasicaulis based on UPLC-ESI-HRMSn, refAbstract=null), Reference(id=1248653101759091600, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601467309937477, doi=null, pmid=null, pmcid=null, year=2013, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[11], rfOrder=10, authorNames=ZHANG T, journalName=Study on the active constituents from Tripterospermum chinense For Anti-H5N1 avian influenza virus, refType=null, unstructuredReference=ZHANG T. Study on the active constituents from Tripterospermum chinense For Anti-H5N1 avian influenza virus[D]. Beijing: Beijing University of Technology, 2013., articleTitle=null, refAbstract=null), Reference(id=1248653101893309332, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601467309937477, doi=null, pmid=null, pmcid=null, year=2021, volume=27, issue=23, pageStart=1, pageEnd=10, url=null, language=null, rfNumber=[12], rfOrder=11, authorNames=LUO S N, PENG Z C, FAN Q, journalName=中国实验方剂学杂志, refType=null, unstructuredReference=LUO S N, PENG Z C, FAN Q, et al. Analysis on chemical constituents in Xiao Chengqitang by UPLC-Q-Orbitrap-MS[J]. Chin J Exp Tradit Med Form( 中国实验方剂学杂志), 2021, 27(23):1-10., articleTitle=Analysis on chemical constituents in Xiao Chengqitang by UPLC-Q-Orbitrap-MS, refAbstract=null), Reference(id=1248653102040109978, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601467309937477, doi=null, pmid=null, pmcid=null, year=2021, volume=27, issue=12, pageStart=157, pageEnd=167, url=null, language=null, rfNumber=[13], rfOrder=12, authorNames=MU B R K·W P E, MA J J, A Z G L·A L M, journalName=中国实验方剂学杂志, refType=null, unstructuredReference=MU B R K·W P E, MA J J, A Z G L·A L M, et al. Screening of anti-inflammatory active sites of water extract of iris halophila root and analysis of its chemical constituents[J]. Chin J Exp Tradit Med Form( 中国实验方剂学杂志), 2021, 27(12):157-167., articleTitle=Screening of anti-inflammatory active sites of water extract of iris halophila root and analysis of its chemical constituents, refAbstract=null), Reference(id=1248653102165939102, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601467309937477, doi=null, pmid=null, pmcid=null, year=2018, volume=49, issue=5, pageStart=1031, pageEnd=1040, url=null, language=null, rfNumber=[14], rfOrder=13, authorNames=ZUO L H, ZHOU L, LI Z L, journalName=中草药, refType=null, unstructuredReference=ZUO L H, ZHOU L, LI Z L, et al. Study on chemical constituents of Tiandan Tongluo Capsule based on UPLC-Q-Orbitrap HRMS[J]. Chin Tradit Herb Drugs( 中草药), 2018, 49(5):1031-1040., articleTitle=Study on chemical constituents of Tiandan Tongluo Capsule based on UPLC-Q-Orbitrap HRMS, refAbstract=null), Reference(id=1248653102258213792, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601467309937477, doi=null, pmid=null, pmcid=null, year=2018, volume=34, issue=1, pageStart=43, pageEnd=47, url=null, language=null, rfNumber=[15], rfOrder=14, authorNames=WANG X, CHEN Z, XIANG G F, journalName=解放军药学学报, refType=null, unstructuredReference=WANG X, CHEN Z, XIANG G F. Chemical compositions in Gentiana scabra and Gentiana macrophylla by UPLC-Q-TOFMS[J]. Pharm J Chin PLA( 解放军药学学报), 2018, 34(1):43-47., articleTitle=Chemical compositions in Gentiana scabra and Gentiana macrophylla by UPLC-Q-TOFMS, refAbstract=null), Reference(id=1248653102367265700, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601467309937477, doi=null, pmid=null, pmcid=null, year=2019, volume=38, issue=2, pageStart=215, pageEnd=218, url=null, language=null, rfNumber=[16], rfOrder=15, authorNames=LIU X Q, HAN H Y, QIN B H, journalName=中南民族大学学报自然科学版, refType=null, unstructuredReference=LIU X Q, HAN H Y, QIN B H, et al. Research on the chemical constituents of Tripterospermum chinense[J]. J South-Central Minzu Univ Nat Sci Edit( 中南民族大学学报自然科学版), 2019, 38(2):215-218., articleTitle=Research on the chemical constituents of Tripterospermum chinense, refAbstract=null), Reference(id=1248653102438568870, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601467309937477, doi=null, pmid=null, pmcid=null, year=2009, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[17], rfOrder=16, authorNames=QI X Y, journalName=and Jusanqi, refType=null, unstructuredReference=QI X Y.Studies on the material foundation and analysis methods of Feixingcao. and Jusanqi[D]. Hangzhou: Zhejiang University, 2009., articleTitle=null, refAbstract=null), Reference(id=1248653102535037863, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601467309937477, doi=null, pmid=null, pmcid=null, year=2018, volume=39, issue=5, pageStart=599, pageEnd=606, url=null, language=null, rfNumber=[18], rfOrder=17, authorNames=ZHANG J M, GUO X Y, QUAN Q H, journalName=质谱学报, refType=null, unstructuredReference=ZHANG J M, GUO X Y, QUAN Q H, et al. Analysis on chemical constituents from Cudrania tricuspidata Bur by LTQ-Orbitrap MS[J]. J Chin Mass Spectr Soc( 质谱学报), 2018, 39(5):599-606., articleTitle=Analysis on chemical constituents from Cudrania tricuspidata Bur by LTQ-Orbitrap MS, refAbstract=null), Reference(id=1248653102648284075, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601467309937477, doi=null, pmid=null, pmcid=null, year=2020, volume=26, issue=07, pageStart=148, pageEnd=155, url=null, language=null, rfNumber=[19], rfOrder=18, authorNames=HU H W, ZHAO Y Y, YANG T L, journalName=中国实验方剂学杂志, refType=null, unstructuredReference=HU H W, ZHAO Y Y, YANG T L, et al. Analysis and identification of chemical constituents of Citri Sarcodactylis Fructus by UPLC-Q-Orbitrap HRMS[J]. Chin J Exp Tradit Med Form( 中国实验方剂学杂志), 2020, 26(07):148-155., articleTitle=Analysis and identification of chemical constituents of Citri Sarcodactylis Fructus by UPLC-Q-Orbitrap HRMS, refAbstract=null), Reference(id=1248653102711198638, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601467309937477, doi=null, pmid=null, pmcid=null, year=2021, volume=48, issue=5, pageStart=175, pageEnd=178, url=null, language=null, rfNumber=[20], rfOrder=19, authorNames=ZAHNG Q Q, FENG Y, YANG G Y, journalName=Guangdong Chem Ind, refType=null, unstructuredReference=ZAHNG Q Q, FENG Y, YANG G Y, et al. Study on chemical constituents of Angelia Sinensis (Oliv) Diels Extracts based on GC-MS[J]. Guangdong Chem Ind (广东化工), 2021, 48(5):175-178., articleTitle=Study on chemical constituents of Angelia Sinensis (Oliv) Diels Extracts based on GC-MS, refAbstract=null), Reference(id=1248653102811861937, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601467309937477, doi=null, pmid=null, pmcid=null, year=2022, volume=57, issue=21, pageStart=1842, pageEnd=1850, url=null, language=null, rfNumber=[21], rfOrder=20, authorNames=DU YQ, LUO L, YANG H, journalName=中国药学杂志, refType=null, unstructuredReference=DU YQ, LUO L, YANG H, et al. Rapid analysis of chemical components in Baoxinning capsule based on UHPLC-LTQ-orbitrap-MS/MS technology[J]. Chin Pharm J( 中国药学杂志), 2022, 57(21):1842-1850., articleTitle=Rapid analysis of chemical components in Baoxinning capsule based on UHPLC-LTQ-orbitrap-MS/MS technology, refAbstract=null)], funds=null, companyList=[AuthorCompany(id=1248653095962563221, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601467309937477, xref=1, ext=[AuthorCompanyExt(id=1248653095970951830, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601467309937477, companyId=1248653095962563221, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1 College of Pharmaceutical Science, Zhejiang Chinese Medical University), AuthorCompanyExt(id=1248653095975146135, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601467309937477, companyId=1248653095962563221, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1 浙江中医药大学药学院, 杭州 310053)]), AuthorCompany(id=1248653096059032219, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601467309937477, xref=2, ext=[AuthorCompanyExt(id=1248653096071615131, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601467309937477, companyId=1248653096059032219, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2 Zhejiang Institute for Food and Drug Control), AuthorCompanyExt(id=1248653096080003741, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601467309937477, companyId=1248653096059032219, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2 浙江省食品药品检验研究院, 杭州 310052)]), AuthorCompany(id=1248653096168084130, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601467309937477, xref=3, ext=[AuthorCompanyExt(id=1248653096176472739, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601467309937477, companyId=1248653096168084130, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=3 NMPA Key Laboratory for Quality Evaluation of Traditional Chinese Medicine(Traditional Chinese Patent Medicine)), AuthorCompanyExt(id=1248653096180667044, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601467309937477, companyId=1248653096168084130, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=3 国家药品监督管理局中成药质量评价重点实验室, 杭州 310052)])], figs=[ArticleFig(id=1248653099020210998, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601467309937477, language=EN, label=Fig.1, caption=Total ion chromatography of T. chinense(postive ion mode)

The numbers in the figure are shown in the corresponding number information in Tab.1.

, figureFileSmall=Yee0v7QFHJc+mtYlAy7c/Q==, figureFileBig=GQzfswAy25FcJZFNJWelYg==, tableContent=null), ArticleFig(id=1248653099125068603, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601467309937477, language=CN, label=图1, caption=肺形草的总离子流图(TIC)(正离子模式)

图中编号见表格1中相对应编号信息。

, figureFileSmall=Yee0v7QFHJc+mtYlAy7c/Q==, figureFileBig=GQzfswAy25FcJZFNJWelYg==, tableContent=null), ArticleFig(id=1248653099234120511, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601467309937477, language=EN, label=Fig.2, caption=TIC of T.chinense(negative ion mode)

The numbers in the figure are shown in the corresponding number information in Tab.1.

, figureFileSmall=jYXTtOVMz/fXcmTrxKTk1Q==, figureFileBig=vbUdtl0EhzPmONTOSaljsQ==, tableContent=null), ArticleFig(id=1248653099309617987, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601467309937477, language=CN, label=图2, caption=肺形草的TIC(负离子模式)

图中编号见表格1中相对应编号信息。

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Identification of chemical constituents of T.chinens by HPLC-LTQ-Orbitrap-MS/MS

, figureFileSmall=null, figureFileBig=null, tableContent=
No. tR
/min		 Compound Formula
m/z		 m/z
(Theoretical)		 m/z
([M+H]+)		 m/z
([M-H]-)		 δ
/×10-6		 m/z
(MS/MS)
1 3.19 Sucrose1)[7-8] C12H22O11 341.108 4 341.108 6 2.2 341.099 6、179.055 9、161.045 4、149.045 4、
143.034 9、119.034 5、113.024 4、101.024 4
2 3.44 Gentiobiose[9] C12H22O11 341.108 4 341.108 8 2.8 179.055 9、113.024 4、89.024 5
3 3.52 Lactose[7]] C12H22O11 341.108 4 341.108 6 2.2 323.097 7、161.045 4、143.034 9、125.024 4、
95.013 9、89.024 5
4 3.52 Venoterpine[7] C9H11NO 150.091 9 150.090 8 -3.5 132.079 8、122.059 1
5 4.3 Citric acid1)[7-8,10] C6H8O7 191.019 2 191.019 8 6.1 173.009 1、154.998 7、129.019 4、111.008 9、
87.009、67.019 2
6 4.47 Uridine[11] C9H12O6N2 243.061 7 243.061 7 2.2 225.051 3、211.036、200.056 2、140.035 3、
111.02、110.024 8
7 5.34 Shanziside[7] C16H24O11 391.124 391.124 2 1.8 185.081 9、167.071 3、149.060 9
8 5.97 Gallic acid1)[12] C7H6O5 169.013 7 169.014 4 7.2 125.024 5、79.300 7
9 6 DL-Phenylalanine[13] C9H11NO2 166.086 8 166.085 8 -2.6 149.058 6、131.048 1、120.079 8
10 6.21 8,10-Dihydroxy sweroside[7] C16H24O10 375.129 1 375.129 3 2.0 169.086 9
11 7.03 Sesamoside[7] C17H24O12 419.118 9 419.119 1 1.8 179.056 1
12 7.07 3-Hydroxy-2-methoxyphenol-1-O-β-D-glucuronic acid[7] C13H16O9 315.071 6 315.072 1 3.2 109.029 6
13 7.65 5-(β-D-Glucopyranosyl)-2-hydroxybenzoic
acid[10]		 C13H16O9 315.071 6 315.071 8 2.4 153.019 3、109.029 6
14 7.86 Loganic acid-11-O-β-glucopyranosyl
ester[8,10]		 C22H34O15 537.181 9 537.181 6 0.3 375.129 1、357.118 6、213.076 6、169.086 9
15 7.95 3, 4-Dihydroxybenzoic acid[11] C7H6O4 153.018 8 153.019 3 7.2 152.356 7、109.029 6
16 8 Morroniside[7] C17H26O11 405.139 7 405.139 7 1.5 179.055 8、161.045 5
17 8.21 Loganic acid[8,10] C16H24O10 375.129 1 375.129 1 1.5 213.076 6、211.097 4、169.087、161.045 5、
151.076 5、133.066、125.060 9、113.024 5、
95.050 4
18 8.42 1-O-Caffeoyl glucose[7] C15H18O9 341.087 3 341.087 5 2.3 179.034 9、135.045 2
19 8.65 8-Epiloganic acid[7] C16H24O10 375.129 1 375.129 1.1 213.076 5、151.076 4
20 9.02 4-Hydroxyphthalide[11] C8H6O3 151.039 5 151.038 3 -4.6 133.027 4、123.043 1、107.048 3
21 9.05 Secologanoside[8,10] C16H22O11 389.108 4 389.108 3 1.1 345.118 6、225.076 6、209.045 4、183.066 2、
165.055 7、121.065 9
22 9.05 Swertiajaposide B[8,10] C15H22O9 345.118 5 345.118 6 1.8 165.055 6、121.065 9、113.024 4、89.024 5
23 9.26 6'-O-β-D-Glucosyl gentiopicroside[8,10] C22H30O14 517.155 7 517.155 5 0.7 221.066 3、193.050 4、179.056、161.045 5、
131.035
24 9.38 4-Hydroxybenzoic acid[11] C7H6O3 137.023 9 137.024 4 7.7 93.034 7
25 9.44 Swertiamarine[8,10] C16H22O10 373.113 4 373.113 2 0.8 211.061、193.050 5、179.056 1、165.055 8、
149.060 8、141.019 4、119.035、113.045 2、
101.024 5
26 9.49 Melilotoside[7] C15H18O8 325.092 3 325.092 4 1.9 163.04、119.050 3
27 9.82 Chlorogenic acid1)[14] C16H18O9 353.087 2 353.087 3 1.6 353.070 3、191.055 9、179.035、173.045 5、
161.024 4、135.045 2
28 10 6-O-Acetyl shanzhiside methyl ester or
8-O-acetyl shanzhiside methyl ester[7]		 C19H28O12 447.150 2 447.150 1 0.9 375.067 1
29 10.5 Swertiapunimarin[8,10] C22H32O14 519.171 4 519.171 3 1.0 179.071 3
30 10.69 Sweroside[8,10] C16H22O9 359.134 2 359.132 7 -2.8 197.079 4、179.069、151.074 2、127.038
31 10.71 Secoxyloganin[9,15] C17H24O11 403.124 403.123 9 0.9 357.118 6、313.128 8、195.066 1、151.076 4
32 10.73 Aesculetin1) (GNPS) C9H6O4 177.018 8 177.019 2 5.5 149.024 4、133.029 5、105.034 7、89.039 8
33 10.95 Caffeic acid[14] C9H8O4 181.05 181.049 -3.0 163.038 9、145.028 4、135.044
34 11.17 8-Epigalloic acid methyl ester[7] C17H26O10 391.160 4 391.158 9 -2.4 373.146 7、211.094 9、193.084 5、179.069 1、
127.037 8
35 11.41 8-Epikingiside[11] C17H24O11 403.124 403.123 9 1.0 179.056、161.045 5、125.024 4、113.024 5
36 11.62 Carlinoside[7] C26H28O15 579.135 579.134 6 0.3 357.061
37 11.77 Secologanin[15] C17H24O10 389.144 8 389.143 3 -2.3 177.053 3、165.053 3、151.037 8
38 11.79 Ethyl caffeate[16] C11H12O4 209.081 4 209.080 2 -3.0 191.069、181.048 3、167.069 1、149.058 6、
135.043 1、109.027 5
39 11.86 Saponarin[8] C27H30O15 595.166 3 595.164 1 -2.8 577.151 5、433.110 8、415.098 8
40 11.86 Isoorientin-7-O-rhamnose[11] C27H30O15 595.166 3 595.164 1 -2.8 559.141 2、541.130 6、523.120 4、499.119 7、
475.120 3
41 11.86 Trifolin[11] C21H20O11 449.108 4 449.106 5 -3.0 287.053 3
42 11.87 2″-O-rhamnopyransoyl-trifoliside[11] C27H30O15 593.150 7 593.150 3 0.3 473.108 4、353.066 4、323.055 6、297.040 5
43 12.07 Isoorientin[9,15] C21H20O11 449.108 4 449.106 7 -2.5 431.094 6、413.084 3、395.073 8、383.074、
353.063 5、329.063 6
44 12.18 Gentiopicroside[8,10] C16H20O9 357.118 6 357.117 3 -2.0 195.063 8、177.053 3、151.037 8、149.058 5
45 12.46 Shanzhiside methyl ester[7-8] C17H26O11 405.139 7 405.139 2 0.2 207.050 8
46 12.9 Lancerin[11] C19H18O10 405.082 2 405.082 2 1.4 387.071 5、369.060 9、351.050 7、315.050 5、
297.039 9
47 13.01 Isovitexin-7-O-rhamnose[11] C27H30O14 579.171 4 579.169 3 -2.7 561.156 6、499.120 4、433.11、415.099 9、
397.089 5、379.078 9、313.068 7
48 13.41 Isovitexin[7,15] C21H20O10 433.113 5 433.112 2 -1.8 415.099 6、397.089 2、379.078 7、367.078 8、
337.068 5、313.068 6、283.058 2
49 13.48 Isorhamnetin-3-O-β-D-glucosyl-4'-O-β-D-glucoside[7] C28H32O17 639.156 1 639.155 6 0.1 477.124、315.071 8
50 13.65 Gmephiloside1)[9] C21H20O12 463.087 6 463.087 7 1.3 445.077、301.034 9、255.029 7
51 13.76 Isoquercitrin1)[9] C21H20O12 463.087 6 463.087 5 0.8 301.034 9、245.045 6、151.003 7
52 13.87 Ferulic acid8,10] C10H10O4 193.050 1 193.050 5 4.8 178.027 1、149.060 9、137.024 4、134.037 4、
93.034 9
53 13.91 Citreorosein[11] C15H10O6 285.039 9 285.040 3 3.1 286.043 4、285.04、257.045 2、241.050 3、
239.034 6、229.050 2、213.055 4、211.040 2、
201.019 2
54 13.94 Luteolin[10] C15H10O6 287.055 5 287.053 4 -2.5 269.042 4、259.058 6、245.042 9、241.047 9、
161.022 1、153.017 1、135.043
55 13.97 (2S,3S,4S,5R,6S)-6-[2-(3,4-Dihydroxyphenyl)-5-hydroxy-4-oxochromen-7-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid2)(GNPS) C21H18O12 461.072 461.072 1 1.3 357.061 2、327.050 7、285.040 1、133.027 1
56 14.33 Loliolide[17] C11H16O3 197.117 8 197.116 2 -2.6 179.105 5、161.095 1、133.100 4、105.069 1、
91.053 5
57 14.64 3,4-di-O-Caffeoylquinic acid2) C25H24O12 515.118 9 515.119 1.1 353.087 2、191.056、179.034 9、173.045 6、
161.021 4、155.034 8、135.042 1
58 15.08 (1S,4aS,7S,7aS)-1-[(2S,3R,4S,5S,6R)-6-[[(E)-3-(3,4-dihydroxyphenyl)prop-2-enoyl]oxymethyl]-3,4,5-trihydroxyoxan-2-yl]oxy-7-hydroxy-7-methyl-4a,5,6,7a-tetrahydro-1H-cyclopenta[c]pyran-4-carboxylic acid2)(GNPS) C25H30O13 537.160 8 537.160 2 0.1 375.129 1、357.097 7、195.066 2、179.034 9、
161.024 4、151.076 6、135.045 2
59 15.47 Aloe emodin[11] C15H10O5 269.045 269.045 1 2.6 151.003 5、149.024 3、107.013 6
60 16.02 1-O-Capryloylsucrose[7] C20H36O12 467.212 8 467.212 9 1.3 421.207 3、289.165 3、161.045 6
61 16.18 Salicylic acid[9] C7H6O3 137.023 9 137.024 4 7.9 93.034 7、65.071 1
62 16.74 10-O-Feruloyl-6″-O-rhamnoyl catalpol[7] C31H40O17 683.218 7 683.218 7 0.8 521.188 4
63 17.59 2,4-Dihydroxy benzyl alcohol[11] C7H8O3 141.055 2 141.054 1 -3.8 123.043 1、109.027 5
64 18.75 Quercetin1)[8,10] C15H10O7 301.034 8 301.034 8 1.6 273.040 1、257.045 2、229.050 4、193.014 1、
178.998 5、151.003 7、121.029 5、107.013 9
65 19.6 1,3,7-Trihydroxy-8-methoxyxanthone[17] C14H10O6 275.055 6 275.054 5 -1.8 245.042 5
66 19.64 Bellidifolin[9] C14H10O6 273.039 9 273.040 2 2.9 258.016 6
67 20.61 Rhodanthenone[9] C14H10O7 289.034 8 289.035 2.4 274.011 6、174.062 6
68 20.68 Apigenin[10] C15H10O5 269.045 269.045 1 2.4 269.044 9、201.055 3、151.003 5、107.013 6
69 20.77 1,3,5-Trihydroxyxanthone[16] C13H8O5 243.029 3 243.029 6 3.3 215.034 8、199.039 9
70 21.13 Diosmetin[10] C16H12O6 299.055 5 299.055 8 2.6 285.035 6、284.032 1
71 21.17 1,3,6,7-Tetrahydroxyxanthone[18] C13H8O6 259.024 2 259.024 5 2.9 258.884、241.047 3、231.029 6
72 21.27 (-)-Pinellic acid[7] C18H34O5 329.232 8 329.233 1 2.6 211.133 8
73 21.89 Apigenin-6-C-xyloside[7] C20H18O9 401.087 2 401.087 2 1.3 121.029 6
74 23.44 (10E)-9,12,13-Trihydroxy-10-octadecenoic
acid[7]		 C18H34O5 329.232 8 329.233 2.3 201.112 9
75 24.73 1,2,8-Trihydroxy-5,6-dimethoxyxanthone[11] C15H12O7 305.066 1 305.065 -1.9 290.039 9、287.053 2
76 25.37 1,7-Dihydroxy-3,8-dimethoxyxanthone[11] C15H12O6 287.055 5 287.055 9 3.1 272.032 2、257.006 4
77 25.37 1,3-Dihydroxy-7,8-dimethoxyxanthone[11] C15H12O6 287.055 5 287.055 9 3.1 272.032 2、257.006 4
78 26.03 1β,2α,3α,24-Tetrahydroxyurs-12-en-28-oic acid[7] C30H48O6 503.337 2 503.337 1 0.7 485.325 4、391.299 6
79 27.45 2'-Acetamido-3'-phenylpropyl 2-benzamido-3-
phenyl propionate[16]		 C27H28N2O4 445.212 7 445.211 -2.7 385.188、252.099 9、224.105 1、194.116、
134.095 2、117.068 8
80 27.61 2α,3β,24-Trihydroxyurs-12-en-28-oic acid[7] C30H48O5 487.342 3 487.342 3 1.1 469.331 6、443.315 6
81 27.8 3β,6α,24-Trihydroxyolean-12-en-28-oic
acid[7]		 C30H48O5 487.342 3 487.342 3 1.0 469.331 6、443.315 6
82 28.77 Glc-Glc-octadecatrienoyl-sn-glycerol2) C33H56O14 675.359 2 675.358 5 0.2 397.134 4、277.216 8、235.082、415.145
83 28.82 Emodin1)[14] C15H10O5 269.045 269.045 2.2 269.044 9、225.055 4
84 28.89 13-HPODE[7] C18H32O4 311.222 2 311.222 4 2.1 293.211 8、249.221 7
85 28.97 8-Hydroxy-1,2,6-trime-thoxyxanthone[11] C16H14O6 303.086 9 303.085 3 -3.2 288.060 8、273.037 5、271.058 3
86 29.26 1β,2α,3α,24-Tetrahydroxyursa-12,20(30)-dien-28-oic acid[7] C30H46O6 501.321 6 501.321 6 1.1 471.310 5
87 31.02 Dibutyl phthalate[16] C16H22O4 277.143 9 277.144 5 3.7 233.154 4、147.008 7、134.037 3、127.112 9、
121.029 5
88 31.54 Quillaic acid[7] C30H46O5 485.326 7 485.326 4 0.6 467.317 2、455.315 9
89 33.69 Maslinic acid[10] C30H48O4 471.347 4 471.347 1 0.5 407.331 3、175.159 6、117.030 8
90 33.99 Corosolic acid[10] C30H48O4 471.347 4 471.347 5 1.3 423.326 1
91 40.14 Oleanolic acid1)[8,10] C30H48O3 455.352 5 455.352 7 1.6 408.334 6、407.331 2
92 40.19 Ursolic acid[8,10] C30H48O3 455.352 5 455.352 6 1.4 455.805 1、272.581 1
), ArticleFig(id=1248653100676961135, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1248601467309937477, language=CN, label=表1, caption=

肺形草高效液相色谱-线性离子阱串联质谱联用技术(HPLC-LTQ-Orbitrap-MS/MS)化学成分鉴定

, figureFileSmall=null, figureFileBig=null, tableContent=
No. tR
/min		 Compound Formula
m/z		 m/z
(Theoretical)		 m/z
([M+H]+)		 m/z
([M-H]-)		 δ
/×10-6		 m/z
(MS/MS)
1 3.19 Sucrose1)[7-8] C12H22O11 341.108 4 341.108 6 2.2 341.099 6、179.055 9、161.045 4、149.045 4、
143.034 9、119.034 5、113.024 4、101.024 4
2 3.44 Gentiobiose[9] C12H22O11 341.108 4 341.108 8 2.8 179.055 9、113.024 4、89.024 5
3 3.52 Lactose[7]] C12H22O11 341.108 4 341.108 6 2.2 323.097 7、161.045 4、143.034 9、125.024 4、
95.013 9、89.024 5
4 3.52 Venoterpine[7] C9H11NO 150.091 9 150.090 8 -3.5 132.079 8、122.059 1
5 4.3 Citric acid1)[7-8,10] C6H8O7 191.019 2 191.019 8 6.1 173.009 1、154.998 7、129.019 4、111.008 9、
87.009、67.019 2
6 4.47 Uridine[11] C9H12O6N2 243.061 7 243.061 7 2.2 225.051 3、211.036、200.056 2、140.035 3、
111.02、110.024 8
7 5.34 Shanziside[7] C16H24O11 391.124 391.124 2 1.8 185.081 9、167.071 3、149.060 9
8 5.97 Gallic acid1)[12] C7H6O5 169.013 7 169.014 4 7.2 125.024 5、79.300 7
9 6 DL-Phenylalanine[13] C9H11NO2 166.086 8 166.085 8 -2.6 149.058 6、131.048 1、120.079 8
10 6.21 8,10-Dihydroxy sweroside[7] C16H24O10 375.129 1 375.129 3 2.0 169.086 9
11 7.03 Sesamoside[7] C17H24O12 419.118 9 419.119 1 1.8 179.056 1
12 7.07 3-Hydroxy-2-methoxyphenol-1-O-β-D-glucuronic acid[7] C13H16O9 315.071 6 315.072 1 3.2 109.029 6
13 7.65 5-(β-D-Glucopyranosyl)-2-hydroxybenzoic
acid[10]		 C13H16O9 315.071 6 315.071 8 2.4 153.019 3、109.029 6
14 7.86 Loganic acid-11-O-β-glucopyranosyl
ester[8,10]		 C22H34O15 537.181 9 537.181 6 0.3 375.129 1、357.118 6、213.076 6、169.086 9
15 7.95 3, 4-Dihydroxybenzoic acid[11] C7H6O4 153.018 8 153.019 3 7.2 152.356 7、109.029 6
16 8 Morroniside[7] C17H26O11 405.139 7 405.139 7 1.5 179.055 8、161.045 5
17 8.21 Loganic acid[8,10] C16H24O10 375.129 1 375.129 1 1.5 213.076 6、211.097 4、169.087、161.045 5、
151.076 5、133.066、125.060 9、113.024 5、
95.050 4
18 8.42 1-O-Caffeoyl glucose[7] C15H18O9 341.087 3 341.087 5 2.3 179.034 9、135.045 2
19 8.65 8-Epiloganic acid[7] C16H24O10 375.129 1 375.129 1.1 213.076 5、151.076 4
20 9.02 4-Hydroxyphthalide[11] C8H6O3 151.039 5 151.038 3 -4.6 133.027 4、123.043 1、107.048 3
21 9.05 Secologanoside[8,10] C16H22O11 389.108 4 389.108 3 1.1 345.118 6、225.076 6、209.045 4、183.066 2、
165.055 7、121.065 9
22 9.05 Swertiajaposide B[8,10] C15H22O9 345.118 5 345.118 6 1.8 165.055 6、121.065 9、113.024 4、89.024 5
23 9.26 6'-O-β-D-Glucosyl gentiopicroside[8,10] C22H30O14 517.155 7 517.155 5 0.7 221.066 3、193.050 4、179.056、161.045 5、
131.035
24 9.38 4-Hydroxybenzoic acid[11] C7H6O3 137.023 9 137.024 4 7.7 93.034 7
25 9.44 Swertiamarine[8,10] C16H22O10 373.113 4 373.113 2 0.8 211.061、193.050 5、179.056 1、165.055 8、
149.060 8、141.019 4、119.035、113.045 2、
101.024 5
26 9.49 Melilotoside[7] C15H18O8 325.092 3 325.092 4 1.9 163.04、119.050 3
27 9.82 Chlorogenic acid1)[14] C16H18O9 353.087 2 353.087 3 1.6 353.070 3、191.055 9、179.035、173.045 5、
161.024 4、135.045 2
28 10 6-O-Acetyl shanzhiside methyl ester or
8-O-acetyl shanzhiside methyl ester[7]		 C19H28O12 447.150 2 447.150 1 0.9 375.067 1
29 10.5 Swertiapunimarin[8,10] C22H32O14 519.171 4 519.171 3 1.0 179.071 3
30 10.69 Sweroside[8,10] C16H22O9 359.134 2 359.132 7 -2.8 197.079 4、179.069、151.074 2、127.038
31 10.71 Secoxyloganin[9,15] C17H24O11 403.124 403.123 9 0.9 357.118 6、313.128 8、195.066 1、151.076 4
32 10.73 Aesculetin1) (GNPS) C9H6O4 177.018 8 177.019 2 5.5 149.024 4、133.029 5、105.034 7、89.039 8
33 10.95 Caffeic acid[14] C9H8O4 181.05 181.049 -3.0 163.038 9、145.028 4、135.044
34 11.17 8-Epigalloic acid methyl ester[7] C17H26O10 391.160 4 391.158 9 -2.4 373.146 7、211.094 9、193.084 5、179.069 1、
127.037 8
35 11.41 8-Epikingiside[11] C17H24O11 403.124 403.123 9 1.0 179.056、161.045 5、125.024 4、113.024 5
36 11.62 Carlinoside[7] C26H28O15 579.135 579.134 6 0.3 357.061
37 11.77 Secologanin[15] C17H24O10 389.144 8 389.143 3 -2.3 177.053 3、165.053 3、151.037 8
38 11.79 Ethyl caffeate[16] C11H12O4 209.081 4 209.080 2 -3.0 191.069、181.048 3、167.069 1、149.058 6、
135.043 1、109.027 5
39 11.86 Saponarin[8] C27H30O15 595.166 3 595.164 1 -2.8 577.151 5、433.110 8、415.098 8
40 11.86 Isoorientin-7-O-rhamnose[11] C27H30O15 595.166 3 595.164 1 -2.8 559.141 2、541.130 6、523.120 4、499.119 7、
475.120 3
41 11.86 Trifolin[11] C21H20O11 449.108 4 449.106 5 -3.0 287.053 3
42 11.87 2″-O-rhamnopyransoyl-trifoliside[11] C27H30O15 593.150 7 593.150 3 0.3 473.108 4、353.066 4、323.055 6、297.040 5
43 12.07 Isoorientin[9,15] C21H20O11 449.108 4 449.106 7 -2.5 431.094 6、413.084 3、395.073 8、383.074、
353.063 5、329.063 6
44 12.18 Gentiopicroside[8,10] C16H20O9 357.118 6 357.117 3 -2.0 195.063 8、177.053 3、151.037 8、149.058 5
45 12.46 Shanzhiside methyl ester[7-8] C17H26O11 405.139 7 405.139 2 0.2 207.050 8
46 12.9 Lancerin[11] C19H18O10 405.082 2 405.082 2 1.4 387.071 5、369.060 9、351.050 7、315.050 5、
297.039 9
47 13.01 Isovitexin-7-O-rhamnose[11] C27H30O14 579.171 4 579.169 3 -2.7 561.156 6、499.120 4、433.11、415.099 9、
397.089 5、379.078 9、313.068 7
48 13.41 Isovitexin[7,15] C21H20O10 433.113 5 433.112 2 -1.8 415.099 6、397.089 2、379.078 7、367.078 8、
337.068 5、313.068 6、283.058 2
49 13.48 Isorhamnetin-3-O-β-D-glucosyl-4'-O-β-D-glucoside[7] C28H32O17 639.156 1 639.155 6 0.1 477.124、315.071 8
50 13.65 Gmephiloside1)[9] C21H20O12 463.087 6 463.087 7 1.3 445.077、301.034 9、255.029 7
51 13.76 Isoquercitrin1)[9] C21H20O12 463.087 6 463.087 5 0.8 301.034 9、245.045 6、151.003 7
52 13.87 Ferulic acid8,10] C10H10O4 193.050 1 193.050 5 4.8 178.027 1、149.060 9、137.024 4、134.037 4、
93.034 9
53 13.91 Citreorosein[11] C15H10O6 285.039 9 285.040 3 3.1 286.043 4、285.04、257.045 2、241.050 3、
239.034 6、229.050 2、213.055 4、211.040 2、
201.019 2
54 13.94 Luteolin[10] C15H10O6 287.055 5 287.053 4 -2.5 269.042 4、259.058 6、245.042 9、241.047 9、
161.022 1、153.017 1、135.043
55 13.97 (2S,3S,4S,5R,6S)-6-[2-(3,4-Dihydroxyphenyl)-5-hydroxy-4-oxochromen-7-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid2)(GNPS) C21H18O12 461.072 461.072 1 1.3 357.061 2、327.050 7、285.040 1、133.027 1
56 14.33 Loliolide[17] C11H16O3 197.117 8 197.116 2 -2.6 179.105 5、161.095 1、133.100 4、105.069 1、
91.053 5
57 14.64 3,4-di-O-Caffeoylquinic acid2) C25H24O12 515.118 9 515.119 1.1 353.087 2、191.056、179.034 9、173.045 6、
161.021 4、155.034 8、135.042 1
58 15.08 (1S,4aS,7S,7aS)-1-[(2S,3R,4S,5S,6R)-6-[[(E)-3-(3,4-dihydroxyphenyl)prop-2-enoyl]oxymethyl]-3,4,5-trihydroxyoxan-2-yl]oxy-7-hydroxy-7-methyl-4a,5,6,7a-tetrahydro-1H-cyclopenta[c]pyran-4-carboxylic acid2)(GNPS) C25H30O13 537.160 8 537.160 2 0.1 375.129 1、357.097 7、195.066 2、179.034 9、
161.024 4、151.076 6、135.045 2
59 15.47 Aloe emodin[11] C15H10O5 269.045 269.045 1 2.6 151.003 5、149.024 3、107.013 6
60 16.02 1-O-Capryloylsucrose[7] C20H36O12 467.212 8 467.212 9 1.3 421.207 3、289.165 3、161.045 6
61 16.18 Salicylic acid[9] C7H6O3 137.023 9 137.024 4 7.9 93.034 7、65.071 1
62 16.74 10-O-Feruloyl-6″-O-rhamnoyl catalpol[7] C31H40O17 683.218 7 683.218 7 0.8 521.188 4
63 17.59 2,4-Dihydroxy benzyl alcohol[11] C7H8O3 141.055 2 141.054 1 -3.8 123.043 1、109.027 5
64 18.75 Quercetin1)[8,10] C15H10O7 301.034 8 301.034 8 1.6 273.040 1、257.045 2、229.050 4、193.014 1、
178.998 5、151.003 7、121.029 5、107.013 9
65 19.6 1,3,7-Trihydroxy-8-methoxyxanthone[17] C14H10O6 275.055 6 275.054 5 -1.8 245.042 5
66 19.64 Bellidifolin[9] C14H10O6 273.039 9 273.040 2 2.9 258.016 6
67 20.61 Rhodanthenone[9] C14H10O7 289.034 8 289.035 2.4 274.011 6、174.062 6
68 20.68 Apigenin[10] C15H10O5 269.045 269.045 1 2.4 269.044 9、201.055 3、151.003 5、107.013 6
69 20.77 1,3,5-Trihydroxyxanthone[16] C13H8O5 243.029 3 243.029 6 3.3 215.034 8、199.039 9
70 21.13 Diosmetin[10] C16H12O6 299.055 5 299.055 8 2.6 285.035 6、284.032 1
71 21.17 1,3,6,7-Tetrahydroxyxanthone[18] C13H8O6 259.024 2 259.024 5 2.9 258.884、241.047 3、231.029 6
72 21.27 (-)-Pinellic acid[7] C18H34O5 329.232 8 329.233 1 2.6 211.133 8
73 21.89 Apigenin-6-C-xyloside[7] C20H18O9 401.087 2 401.087 2 1.3 121.029 6
74 23.44 (10E)-9,12,13-Trihydroxy-10-octadecenoic
acid[7]		 C18H34O5 329.232 8 329.233 2.3 201.112 9
75 24.73 1,2,8-Trihydroxy-5,6-dimethoxyxanthone[11] C15H12O7 305.066 1 305.065 -1.9 290.039 9、287.053 2
76 25.37 1,7-Dihydroxy-3,8-dimethoxyxanthone[11] C15H12O6 287.055 5 287.055 9 3.1 272.032 2、257.006 4
77 25.37 1,3-Dihydroxy-7,8-dimethoxyxanthone[11] C15H12O6 287.055 5 287.055 9 3.1 272.032 2、257.006 4
78 26.03 1β,2α,3α,24-Tetrahydroxyurs-12-en-28-oic acid[7] C30H48O6 503.337 2 503.337 1 0.7 485.325 4、391.299 6
79 27.45 2'-Acetamido-3'-phenylpropyl 2-benzamido-3-
phenyl propionate[16]		 C27H28N2O4 445.212 7 445.211 -2.7 385.188、252.099 9、224.105 1、194.116、
134.095 2、117.068 8
80 27.61 2α,3β,24-Trihydroxyurs-12-en-28-oic acid[7] C30H48O5 487.342 3 487.342 3 1.1 469.331 6、443.315 6
81 27.8 3β,6α,24-Trihydroxyolean-12-en-28-oic
acid[7]		 C30H48O5 487.342 3 487.342 3 1.0 469.331 6、443.315 6
82 28.77 Glc-Glc-octadecatrienoyl-sn-glycerol2) C33H56O14 675.359 2 675.358 5 0.2 397.134 4、277.216 8、235.082、415.145
83 28.82 Emodin1)[14] C15H10O5 269.045 269.045 2.2 269.044 9、225.055 4
84 28.89 13-HPODE[7] C18H32O4 311.222 2 311.222 4 2.1 293.211 8、249.221 7
85 28.97 8-Hydroxy-1,2,6-trime-thoxyxanthone[11] C16H14O6 303.086 9 303.085 3 -3.2 288.060 8、273.037 5、271.058 3
86 29.26 1β,2α,3α,24-Tetrahydroxyursa-12,20(30)-dien-28-oic acid[7] C30H46O6 501.321 6 501.321 6 1.1 471.310 5
87 31.02 Dibutyl phthalate[16] C16H22O4 277.143 9 277.144 5 3.7 233.154 4、147.008 7、134.037 3、127.112 9、
121.029 5
88 31.54 Quillaic acid[7] C30H46O5 485.326 7 485.326 4 0.6 467.317 2、455.315 9
89 33.69 Maslinic acid[10] C30H48O4 471.347 4 471.347 1 0.5 407.331 3、175.159 6、117.030 8
90 33.99 Corosolic acid[10] C30H48O4 471.347 4 471.347 5 1.3 423.326 1
91 40.14 Oleanolic acid1)[8,10] C30H48O3 455.352 5 455.352 7 1.6 408.334 6、407.331 2
92 40.19 Ursolic acid[8,10] C30H48O3 455.352 5 455.352 6 1.4 455.805 1、272.581 1
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基于HPLC-LTQ-Orbitrap-MS/MS技术快速分析肺形草中的化学成分
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张艺 1 , 谭春梅 2, 3 , 马临科 2, 3, * , 郑成 2, 3 , 陈碧莲 2, 3
中国药学杂志 | 研究论文 2024,59(6): 491-500
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中国药学杂志 | 研究论文 2024, 59(6): 491-500
基于HPLC-LTQ-Orbitrap-MS/MS技术快速分析肺形草中的化学成分
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张艺1, 谭春梅2, 3, 马临科2, 3, *, 郑成2, 3, 陈碧莲2, 3
作者信息
  • 1 浙江中医药大学药学院, 杭州 310053
  • 2 浙江省食品药品检验研究院, 杭州 310052
  • 3 国家药品监督管理局中成药质量评价重点实验室, 杭州 310052

    • 张艺,女,硕士研究生 研究方向:中药药效物质基础研究和质量分析评价,研究方向:中药药效物质基础研究和质量分析评价 Tel:(0571)87180305

通讯作者:

*马临科,男,副主任,中药师
Rapid Analysis of Chemical Components in Tripterospermum chinense (Migo)H. Smith Based on HPLC-LTQ-Orbitrap-MS/MS Technology
Yi ZHANG1, Chunmei TAN2, 3, Linke MA2, 3, *, Cheng ZHENG2, 3, Bilian CHEN2, 3
Affiliations
  • 1 College of Pharmaceutical Science, Zhejiang Chinese Medical University
  • 2 Zhejiang Institute for Food and Drug Control
  • 3 NMPA Key Laboratory for Quality Evaluation of Traditional Chinese Medicine(Traditional Chinese Patent Medicine)
出版时间: 2024-03-22
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摘要
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目的 建立高效、稳定的高效液相色谱-线性离子阱串联质谱联用技术(HPLC-LTQ-Orbitrap-MS/MS)方法,对肺形草全草的化学成分快速识别和鉴定。方法 采用HPLC-LTQ-Orbitrap-MS/MS对肺形草中的化学成分进行快速分析。通过HPLC-LTQ-Orbitrap-MS/MS扫描得到的化合物相对分子质量,对照品和多级碎片离子信息,并结合相关文献以及裂解规律,从而实现对肺形草的准确定性分析。结果 共鉴定出92种化学成分,包括黄酮类、萜类、有机酸类、蒽醌类、糖类、酯类等,其中化合物3,4-di-O-caffeoylquinic acid、(2S,3S,4S,5R,6S)-6-[2-(3,4-dihydroxyphenyl)-5-hydroxy-4-oxochromen-7-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid、(1S,4aS,7S,7aS)-1-[(2S,3R,4S,5S,6R)-6-[[(E)-3-(3,4-dihydroxyphenyl)prop-2-enoyl]oxymethyl]-3,4,5-trihydroxyoxan-2-yl]oxy-7-hydroxy-7-methyl-4a,5,6,7a-tetrahydro-1H-cyclopenta[c]pyran-4-carboxylic acid和Glc-Glc-octadecatrienoyl-sn-glycerol经全球天然产物社会分子网络系统(GNPS)数据库比对,为首次在该植物中鉴别得到。结论 建立了对肺形草中化学成分的快速定性分析的方法,为肺形草化学成分的提取分离及其药效物质基础提供科学依据,并为其质量评价和应用开发提供了一定的参考。

肺形草  /  高效液相色谱-线性离子阱串联质谱联用技术  /  黄酮  /  萜  /  有机酸  /  蒽醌  /  酯

OBJECTIVE An efficient and stable HPLC-LTQ-Orbitrap-MS/MS method was developed to analyze and identify the chemical constituents from Tripterospermum chinense (Migo)H.Smith rapidly. METHODS The chemical components in T.chinense were analyzed by high performance liquid chromatography linear ion trap orbital trap tandem mass spectrometry (HPLC-LTQ-Orbitrap-MS/MS). HPLC-LTQ-Orbitrap-MS/MS was used to analyze the chemical components in T.chinense quickly. The accurate molecular weight, reference substance and multistage fragment ion information of the compounds obtained by HPLC-LTQ-Orbitrap-MS/MS scanning, combined with relevant literature and fragmentation regularity, were used to achieve the accurate qualitative analysis of T. chinense. RESULTS A total of 92 chemical constituents were identified, including flavonoids, terpenoids, organic acids, anthraquinones, saccharide, ester and the others. Among them, compounds 3, 4-di-O-caffeoylquinic acid、(2S, 3S, 4S, 5R, 6S)-6-[2-(3, 4-dihydroxyphenyl)-5-hydroxy-4-oxochromen-7-yl]oxy-3, 4, 5-trihydroxyoxane-2-carboxylic acid、(1S, 4aS, 7S, 7aS)-1-[(2S, 3R, 4S, 5S, 6R)-6-[[(E)-3-(3, 4-dihydroxyphenyl)prop-2-enoyl]oxymethyl]-3, 4, 5-trihydroxyoxan-2-yl]oxy-7-hydroxy-7-methyl-4a, 5, 6, 7a-tetrahydro-1H-cyclopenta[c]pyran-4-carboxylic acid and Glc-Glc-octadecatrienoyl-sn-glycerol were identified for the first time in this plant by GNPS database alignment. CONCLUSION A rapid qualitative analysis method of chemical components in T.chinense is established, which provides a scientific basis for the extraction and isolation of the chemical constituents of T.chinense and its pharmacodynamic material basis, and provides a certain reference significance for its quality evaluation and application development.

Tripterospermum chinense  /  HPLC-LTQ-Orbitrap-MS/MS  /  flavonoid  /  terpenoid  /  organic acid  /  anthraquinone  /  ester
张艺, 谭春梅, 马临科, 郑成, 陈碧莲. 基于HPLC-LTQ-Orbitrap-MS/MS技术快速分析肺形草中的化学成分. 中国药学杂志, 2024 , 59 (6) : 491 -500 .
Yi ZHANG, Chunmei TAN, Linke MA, Cheng ZHENG, Bilian CHEN. Rapid Analysis of Chemical Components in Tripterospermum chinense (Migo)H. Smith Based on HPLC-LTQ-Orbitrap-MS/MS Technology[J]. Chinese Pharmaceutical Journal, 2024 , 59 (6) : 491 -500 .
正文
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肺形草为龙胆科双蝴蝶属植物双蝴蝶[Tripterospermum chinense (Migo) H. Smith]的幼嫩全草,因形似两叶肺,故名为肺形草,首载于《药用植物图说》,又称双蝴蝶、穿藤金兰花、蝴蝶草、胡地莲等。肺形草是多年生缠绕草本,生于海拔300~1 100 m的山坡林下、灌木丛或草丛中,多为阴暗、潮湿之处,主要分布于江苏、安徽、浙江、江西、福建、湖南、广西等地[1]。
其味辛、甘,性寒;归肺、肾经。具有清热解毒,祛痰止咳等功能[2],主治肺热咳嗽、肺痨咯血、肺痈、肾炎、乳痈、疮痈疔肿、创伤出血、毒蛇咬伤等[3]。
目前双蝴蝶属植物化学成分的研究大多集中在玉山双蝴蝶(T. lanceolatum)、台湾双蝴蝶(T. taiwanense)和日本双蝴蝶(T. japonicum)等几个种,但对肺形草(T. chinense)化学成分研究相对较少[3]。在之前的研究中[4-6],肺形草化学成分的鉴别均为先进行色谱硅胶柱分离,之后再进行成分鉴定,这种传统的中药化学成分研究方法花费的时间长且效率低,并且消耗的中药材数量也较多。因此,本研究采用高效液相色谱-线性离子阱串联质谱联用技术(HPLC-LTQ-Orbitrap-MS/MS),为系统地分析肺形草的化学成分建立一个科学、稳定、准确性好、灵敏度高的定性分析方法,并通过UHPLC-LTQ-Orbitrap-MS/MS扫描得到的化合物精确相对分子质量,对照品和多级碎片离子信息,结合相关文献以及裂解规律,快速分析鉴定化合物。该方法为肺形草药材的质量标准提升提供了科学理论依据和技术支持。
1 仪器与材料
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1.1 仪器
Thermo Dionex UltiMate 3000快速液相色谱仪(HPLC),Thermo LTQ Orbitrap Velos Pro质谱仪(美国Thermo Fisher Scientific公司)。乙腈为色谱纯试剂,甲酸为色谱纯试剂,试验用水为Milli-Q系统制备。
1.2 材料
槲皮素(批号:100081-201610,纯度99.1%)、金丝桃苷(批号:111521-201809,纯度94.3%)、异槲皮苷(批号:111809-201804,纯度97.2%)、没食子酸(批号:110831-201906,纯度90.5%)、绿原酸(批号:110753-201716,纯度99.3%)、蔗糖(批号:111507-202105)、秦皮乙素(批号:110741-201708,纯度99.9%)、大黄素(批号:110756-201913,纯度96%)、柠檬酸(批号:111679-201602,纯度97%)、齐墩果酸(批号:110709-201808,纯度95.8%)、咖啡酸(批号:110885-201703,纯度99.7%),以上对照品均购于中国食品药品检定研究院。肺形草购于浙江中医药大学中药饮片有限公司,产地为主产地之一,经浙江省食品药品检验研究院郭增喜主任中药师鉴定为龙胆科双蝴蝶属植物双蝴蝶[Tripterospermum chinese (Migo) H.Smith]的幼嫩全草,本批性状及化学成分具有一定的代表性。
2 方法与结果
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2.1 色谱条件
Welch Ultimate AQ C18色谱柱(4.6 mm×250 mm,5μm);柱温30 ℃;流动相A为0.1%甲酸,流动相B为乙腈;梯度洗脱(0~45 min,5%~100%B;45~50 min,100%B),流速为1 mL·min-1;检测波长为254nm。正、负离子模式下进样量均为5 μL。
2.2 质谱条件
电喷雾离子源(ESI),高分辨检测模式(FTMS),分辨率60000 FWHM,离子源参数:离子源温度:350 ℃;鞘气流速:35 L·min-1;辅助气流速:10 L·min-1;反吹气流速:0 L·min-1;I Spray电压:4.0 kV;毛细管温度:275 ℃;采集模式为正、负离子模式;质谱扫描范围m/z 100~1 700。
2.3 对照品溶液的制备
取“1.2”项下对照品各约1.0 mg,精密称定,分别置于100 mL量瓶中,加入体积分数70%甲醇溶解并稀释至刻度,摇匀,制备成质量浓度为10 μg·mL-1的单一对照品储备液。
2.4 供试品溶液的制备
取本品粉末(过三号筛)约1 g,精密称定,置具塞锥形瓶中,精密加入体积分数70%甲醇25 mL,密塞,称定重量,超声处理(功率250 W,频率40 kHz)30 min,放冷,再称定重量,用体积分数70%甲醇补足减失的重量,摇匀,滤过,取续滤液,既得。
2.5 HPLC-LTQ-Orbitrap-MS/MS成分鉴定
通过对照品及二级质谱特征解析,结合文献,共检测出92种化合物,见表1。按“2.1”和“2.2“项下的条件进行检测,获得肺形草的HPLC-LTQ-Orbitrap-MS/MS的正、负总离子流图,见图1、2。
2.6 有关化合物的鉴定与归属
2.6.1 黄酮类
黄酮类化合物原指基本母核为2-苯基色原酮类化合物,现在则泛指两个具有酚羟基的苯环通过中央三碳原子相互连接而成即C6-C3-C6结构的一系列化合物,包括黄酮的同分异构体及其氧化还原产物。但在自然界中,黄酮类化合物大多以苷类形式存在,主要为黄酮糖苷或黄酮苷元,因此,黄酮苷元类化合物在质谱中主要为丢失CO、CO2、CH3等碎片及发生RDA裂解而形成一系列特征离子峰;黄酮苷类化合物则为丢失糖基而形成丰度较高的黄酮苷元[19]。
化合物55,根据高分辨质谱数据得到其准分子离子峰(m/z 461.072 1[M-H]-)和二级碎片离子(m/z 357.061 2[M-H-C3H4O4]-、m/z 327.050 7[M-H-C3H4O4-CH2O]-、m/z 285.040 1[M-H-C6H8O6]-、m/z 133.027 1),推测分子式为C21H18O12,结合GNPS数据库比对和碎片离子信息,推测可能的裂解途径,二级质谱图和裂解途径见图3,其中碎片离子133.027 1是准分子离子通过RDA裂解得到的,初步鉴定该化合物为(2S,3S,4S,5R,6S)-6-[2-(3,4-dihydroxyphenyl)-5-hydroxy-4-oxochromen-7-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid,也为肺形草中首次鉴别得到。
此外,肺形草中黄酮类的化合物有一类结构较为特殊的化合物,即呫吨酮类,也就是苯并色原酮类,是一种杂环有机化合物。呫吨酮类化合物母核结构较为稳定,质谱裂解中多丢失母核上连接的-CH3、H2O等分子。
化合物86,根据高分辨质谱数据得到其准分子离子峰(m/z 303.084 3[M-H]-)和二级碎片离子(271.058 3[M+H-CH3OH]+、288.060 8[M+H-CH3]+、273.037 5[M+H-2CH3]+),推测分子式为C16H14O6,结合相关文献[15]和碎片离子信息,推测可能的裂解途径,二级质谱图和裂解途径见图4,初步鉴定该化合物为8-羟基-1,2,6-三甲氧基呫吨酮。
2.6.2 萜类
萜类化合物由于基本母核多,无稳定的芳香环、芳杂环及脂杂环结构系统,大多缺乏“定向”裂解基团,因而在电子轰击下能够裂解的化学键较多,重排屡屡发生,裂解方式复杂。肺形草中的萜类化合物多为环烯醚萜类化合物,其基本碳架包括环戊烷环烯醚萜和环戊烷开裂的裂环环烯醚萜两种,但由于环烯醚萜类的1位半缩醛羟基不稳定,故主要以糖苷形式存在。因此,在质谱裂解中此类化合物多先丢失糖基产生碎片离子,进而再进一步裂解形成其他碎片离子。
化合物24,根据高分辨质谱数据得到其准分子离子峰(m/z 373.113 4[M-H]-)和二级碎片离子(211.061[M-H-C6H10O5]-、193.050 5 [M-H-C6H12O6]-、149.060 8 [M-H-C6H12O6-CO2]-),推测分子式为C16H22O10,结合相关文献[9,11]和碎片离子信息,推测可能的裂解途径,二级质谱图和裂解途径见图5,初步鉴定该化合物为獐芽菜苦苷。
2.6.3 有机酸类
有机酸类化合物是一种广泛存在于自然界植物中的酸性有机化合物,具有很强的抗氧化和抗菌、消炎等药理作用,其在质谱中容易丢失CO、CO2、-COOH、H2O等而产生碎片离子峰[20]。
化合物57,根据高分辨质谱数据得到其准分子离子峰(m/z 515.119[M-H]-)和二级碎片离子(353.087 2[M-H-C9H6O3]-、191.056[M-H-2C9H6O3]-、173.045 6[M-H-2C9H6O3-H2O]-、173.045 6[M-H-2C9H6O3-2H2O]-),推测分子式为C25H24O12,结合GNPS数据库比对和碎片离子信息,推测可能的裂解途径,二级质谱图和裂解途径见图6,初步鉴定该化合物为3,4-di-O-caffeoylquinic acid,也为肺形草中首次鉴别得到。
2.6.4 蒽醌类
天然蒽醌类化合物以9,10-蒽醌最为常见,且以游离苷元及糖苷两种形式存在于植物体内。在蒽醌母核上常有羟基、羟甲基、甲氧基和羧基取代。游离蒽醌类化合物在质谱碰撞中常依次脱去两分子的CO,得到[M-28]、[M-56]的碎片离子。
化合物53,根据高分辨质谱数据得到其准分子离子峰(m/z 285.040 3[M-H]-)和二级碎片离子(257.045 2[M-H-CO]-、241.050 3[M-H-CO2]-、229.050 2[M-H-2CO]-、 213.055 4[M-H-CO2-CO]-、201.019 2[M-H-3CO]-),推测分子式为C15H10O6,结合相关文献[15]和碎片离子信息,推测可能的裂解途径,二级质谱图和裂解途径见图7,初步鉴定该化合物为ω-羟基大黄素。
2.6.5 酯类
酯类化合物是酸(羧酸或无机含氧酸)与醇起反应生成的一类有机化合物,在质谱裂解中,酯键连接处通常为优先断裂处,其次再丢失基团上的H2O、CO2等中性分子基团而产生碎片离子峰。
化合物58,根据高分辨质谱数据得到其准分子离子峰(m/z 537.160 2[M-H]-)和二级碎片离子(375.129 1[M-H-C9H6O3]-、357.097 7[M-H-C9H6O3-H2O]-、179.034 9[M-H-C16H22O9]-、161.024 4[M-H-C16H22O9-H2O]-、135.045 2[M-H-C16H22O9-CO2]-),推测分子式为C25H30O13,结合GNPS数据库比对和碎片离子信息,推测可能的裂解途径,二级质谱图和裂解途径见图8,初步鉴定该化合物为(1S,4aS,7S,7aS)-1-[(2S,3R,4S,5S,6R)-6-[[(E)-3-(3,4-dihydroxyphenyl)prop-2-enoyl]oxymethyl]-3,4,5-trihydroxyoxan-2-yl]oxy-7-hydroxy-7-methyl-4a,5,6,7a-tetrahydro-1H-cyclopenta[c]pyran-4-carboxylic acid,也为肺形草中首次鉴别得到。
3 讨论
收起
与传统的LC/MS相比,线性离子阱-静电场轨道阱联用质谱(LTQ-Orbitrap MS)利用其独特的结构能够在静电场轨道阱(Orbitrap)和线性离子阱(LTQ)质量分析器中实现同时的母离子隔离,裂解与数据采集,从而获得更高质量的数据[21]。相比以往肺形草化学成分的研究,本研究能更加快速、高效、高灵敏度地分析其化学成分,同时在缺少对照品的情况下,也能较为全面地进行结构鉴定。除此以外,传统中药化学成分分析药材用量较大,而肺形草野生品产量相对较少,利用液质联用技术能大大减少药材的消耗,为肺形草后续持续性发展奠定基础。
本研究利用UHPLC-LTQ-Orbitrap-MS/MS技术分析了肺形草的化学成分,根据正反离子模式下准分子离子峰,再结合文献查阅与对照品的比对,共初步鉴定了92个化学成分,包括25个黄酮类成分,21个萜类成分,24个有机酸类成分,3个蒽醌类成分,5个糖类成分,6个酯类成分以及其他成分,其中化合物3,4-di-O-caffeoylquinic acid、(2S,3S,4S,5R,6S)-6-[2-(3,4-dihydroxyphenyl)-5-hydroxy-4-oxochromen-7-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid、(1S,4aS,7S,7aS)-1-[(2S,3R,4S,5S,6R)-6-[[(E)-3-(3,4-dihydroxyphenyl)prop-2-enoyl]oxymethyl]-3,4,5-trihydroxyoxan-2-yl]oxy-7-hydroxy-7-methyl-4a,5,6,7a-tetrahydro-1H-cyclopenta[c]pyran-4-carboxylic acid和Glc-Glc-octadecatrienoyl-sn-glycerol经GNPS数据库比对,为首次在该植物中鉴别得到。本研究为肺形草的质量评价指标及药效物质基础研究提供了一定参考。
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参考文献 引证文献
排序方式：
[1]
LIN Q. Notes on Gentianaceae From South China[J]. J Trop Subtrop Bot( 热带亚热带植物学报), 1997(2):13-15.
[2]
Ch.P(1977)Vol Ⅰ(中国药典1977年版.一部)[S].1977:366.
[3]
SONG L R. Chin Herbal Med(中华本草)[M]. 1999:6266-6267.
[4]
ZUO Y M, LIU D H, ZHANG Z L, et al. Study on chemical components of Tripterospermum chinese[J]. J Chin Med Mater( 中药材), 2014, 37(11):2002-2004.
[5]
ZHANG T, LI B, CHEN L, et al. Chemical constituents of Tripterospermum chinese[J]. Mil Med Sci( 军事医学), 2012, 36(12):920-924.
[6]
ZHANG T, LI B, CHEN L, et al. A novel lactone from Tripterospermum chinense[J]. Acta Pharm Sin( 药学学报), 2012, 47(11):1517-1520.
[7]
LIANG X P, JI S, DU S B, et al. Analysis of chemical constituents in different parts of Gentiana straminea Based on UPLC-Q-TOF-MS/MS[J]. Chin J Exp Tradit Med Form( 中国实验方剂学杂志), 2022, 28(8):139-148.
[8]
WANG C S, DONG H J, BAO Y T, et al. Rapid analysis on chemical constituents from roots of Gentiana crasicaulis by ultra-high performance liquid chromatography coupled with hybrid quadrupole-orbitrap mass spectrometry[J]. Chin Tradit Herb Drugs( 中草药), 2016, 47(18):3175-3180.
[9]
LUO J, ZHAO L J, BAO J P, et al. Analysis of chemical compositions in Gentiana rhodantha by UPLC-Q-TOF-MS[J]. Chin J Exp Tradit Med Form( 中国实验方剂学杂志), 2018, 24(24):89-94.
[10]
CHEN J K, ZENG R. Application of metabolomics approach to study on chemical constituents in different parts of Gentiana crasicaulis based on UPLC-ESI-HRMSn[J]. Chin Tradit Herb Drugs( 中草药), 2018, 49(10):2328-2335.
[11]
ZHANG T. Study on the active constituents from Tripterospermum chinense For Anti-H5N1 avian influenza virus[D]. Beijing: Beijing University of Technology, 2013.
[12]
LUO S N, PENG Z C, FAN Q, et al. Analysis on chemical constituents in Xiao Chengqitang by UPLC-Q-Orbitrap-MS[J]. Chin J Exp Tradit Med Form( 中国实验方剂学杂志), 2021, 27(23):1-10.
[13]
MU B R K·W P E, MA J J, A Z G L·A L M, et al. Screening of anti-inflammatory active sites of water extract of iris halophila root and analysis of its chemical constituents[J]. Chin J Exp Tradit Med Form( 中国实验方剂学杂志), 2021, 27(12):157-167.
[14]
ZUO L H, ZHOU L, LI Z L, et al. Study on chemical constituents of Tiandan Tongluo Capsule based on UPLC-Q-Orbitrap HRMS[J]. Chin Tradit Herb Drugs( 中草药), 2018, 49(5):1031-1040.
[15]
WANG X, CHEN Z, XIANG G F. Chemical compositions in Gentiana scabra and Gentiana macrophylla by UPLC-Q-TOFMS[J]. Pharm J Chin PLA( 解放军药学学报), 2018, 34(1):43-47.
[16]
LIU X Q, HAN H Y, QIN B H, et al. Research on the chemical constituents of Tripterospermum chinense[J]. J South-Central Minzu Univ Nat Sci Edit( 中南民族大学学报自然科学版), 2019, 38(2):215-218.
[17]
QI X Y.Studies on the material foundation and analysis methods of Feixingcao. and Jusanqi[D]. Hangzhou: Zhejiang University, 2009.
[18]
ZHANG J M, GUO X Y, QUAN Q H, et al. Analysis on chemical constituents from Cudrania tricuspidata Bur by LTQ-Orbitrap MS[J]. J Chin Mass Spectr Soc( 质谱学报), 2018, 39(5):599-606.
[19]
HU H W, ZHAO Y Y, YANG T L, et al. Analysis and identification of chemical constituents of Citri Sarcodactylis Fructus by UPLC-Q-Orbitrap HRMS[J]. Chin J Exp Tradit Med Form( 中国实验方剂学杂志), 2020, 26(07):148-155.
[20]
ZAHNG Q Q, FENG Y, YANG G Y, et al. Study on chemical constituents of Angelia Sinensis (Oliv) Diels Extracts based on GC-MS[J]. Guangdong Chem Ind (广东化工), 2021, 48(5):175-178.
[21]
DU YQ, LUO L, YANG H, et al. Rapid analysis of chemical components in Baoxinning capsule based on UHPLC-LTQ-orbitrap-MS/MS technology[J]. Chin Pharm J( 中国药学杂志), 2022, 57(21):1842-1850.
2024年第59卷第6期
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  • 接收时间：2023-03-04
  • 首发时间：2026-04-08
  • 出版时间：2024-03-22
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  • 收稿日期：2023-03-04
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    1 浙江中医药大学药学院, 杭州 310053
    2 浙江省食品药品检验研究院, 杭州 310052
    3 国家药品监督管理局中成药质量评价重点实验室, 杭州 310052

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*马临科,男,副主任,中药师
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2种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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