首页 期刊 论文 学科刊群 资讯 加盟 关于
EN
image
Article(id=1200147897519014231, tenantId=1146029695717560320, journalId=1190317699101192196, issueId=1200147892095779072, articleNumber=1001-2494(2024)11-1041-06, orderNo=null, doi=10.11669/cpj.2024.11.011, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1702224000000, receivedDateStr=2023-12-11, revisedDate=null, revisedDateStr=null, acceptedDate=null, acceptedDateStr=null, onlineDate=1764067156438, onlineDateStr=2025-11-25, pubDate=1717776000000, pubDateStr=2024-06-08, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1764067156438, onlineIssueDateStr=2025-11-25, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1764067156438, creator=13701087609, updateTime=1764067156438, updator=13701087609, issue=Issue{id=1200147892095779072, tenantId=1146029695717560320, journalId=1190317699101192196, year='2024', volume='59', issue='11', pageStart='953', pageEnd='1064', issueExtLink='null', onlineDate='null', pubDate='null', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1764067155144, creator=13701087609, updateTime=1764067375019, updator=13701087609, preIssue=null, nextIssue=null, ext={EN=IssueExt(id=1200148814364508515, tenantId=1146029695717560320, journalId=1190317699101192196, issueId=1200147892095779072, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1200148814364508516, tenantId=1146029695717560320, journalId=1190317699101192196, issueId=1200147892095779072, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null}, startPage=1041, endPage=1046, ext={EN=ArticleExt(id=1200147897846169962, articleId=1200147897519014231, tenantId=1146029695717560320, journalId=1190317699101192196, language=EN, title=Evaluation of the Application Value of Metagenomic Sequencing in Identification and Tracing of Bacteria Contaminating Drugs, columnId=null, journalTitle=Chinese Pharmaceutical Journal, columnName=null, runingTitle=null, highlight=null, articleAbstract=

OBJECTIVE To investigate metagenomic sequencing in the identification and tracing of bacteria contaminating drugs, and evaluate its application value. METHODS Both metagenomic sequencing and isolation culturing were employed to identify and type bacteria in two batches of Chinese patent medicines and environmental samples from the testing laboratory. The metagenomic sequencing results were analyzed at the strain level using MetaPhlan and StrainSifter software. RESULTS The analysis revealed a substantial contamination of Bacillus cereus-like organisms in both medicines and environmental samples, with the presence of the same B. cereus-like strain in medicine B18 and environmental sample HJ2. Through isolation culturing, five B. cereus-like strains were obtained from the medicines and environment. A phylogenetic tree was constructed based on the gyrA and panC gene sequences amplified by B. cereus-specific PCR, and multilocus sequence typing (MLST) results, all of which demonstrated that the B. cereus-like strain in medicine B18 belonged to the same group as one strain from the environment. CONCLUSION These findings indicate that metagenomic sequencing technology is reliable and has advantage over isolation culturing in comprehensively detecting potentially hazardous bacterial species and accurately performing strain-level traceability analysis. It holds significant application value in identification and tracing of pharmaceutical contamination.

, correspAuthors=null, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, authorCompany=null, fund=null, authors=null, authorsList=Zhuoyan YAN, Yuxiang TAN, Jiheng LIN, Yingying XIA, Ying SUN, Jie CUI, Zhuliang HUANG), CN=ArticleExt(id=1200147898710196629, articleId=1200147897519014231, tenantId=1146029695717560320, journalId=1190317699101192196, language=CN, title=宏基因组测序法在药品污染细菌鉴定与溯源中的应用研究, columnId=1190352405612040510, journalTitle=中国药学杂志, columnName=论著, runingTitle=null, highlight=null, articleAbstract=

目的 评价宏基因组测序法在药品污染细菌鉴定和溯源中的应用。方法 利用宏基因组测序法和分离培养法同时对两批中成药及检测实验室环境取样中的细菌进行鉴定和分型。宏基因组测序结果通过MetaPhlan和StrainSifter软件进行菌株水平的分析。结果 经宏基因测序发现,药品与环境中均有大量的蜡样芽孢杆菌污染,且药品B18与环境样本HJ2中含有相同的蜡样芽孢杆菌菌组。培养法从药品和环境中共分离得到的5株蜡样芽孢杆菌,通过蜡样芽孢杆菌特异性聚合酶链式反应(PCR)扩增基因gyrA和panC序列构建NJ进化树,以及多位点序列分型(MLST),结果均显示药品B18中的蜡样芽孢杆菌菌株与环境样本HJ2的菌株同组。结论 宏基因组测序技术是可靠的,且相比培养法,能够更全面发现具有潜在风险的细菌物种,同时也能准确地进行菌株水平的溯源分析,在药品污染菌鉴定与溯源中具有重要的应用价值。

, correspAuthors=null, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=hFMDHXLoU3xW/cbjiqEgTA==, magXml=I5c7vFLoZYdd5gJlHK5TKQ==, pdfUrl=null, pdf=ymK0mTIsW5I0R7g9ldCzEA==, pdfFileSize=1548950, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=SKv1SwBcWOiGK0EnLnnnlA==, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=9FsQOYTPmAh47Bh1N3nJEA==, mapNumber=null, authorCompany=null, fund=null, authors=

严卓彦,女,硕士 研究方向:药品微生物检测研究 Tel:(0580)2187150

, authorsList=严卓彦, 谭宇翔, 林吉恒, 夏瑛瑛, 孙瑛, 崔洁, 黄朱梁)}, authors=[Author(id=1200147899167375802, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147897519014231, orderNo=0, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1200147899473560000, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147897519014231, authorId=1200147899167375802, language=EN, stringName=Zhuoyan YAN, firstName=Zhuoyan, middleName=null, lastName=YAN, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, address=1 National Ocean Food Quality Inspection Center, Zhoushan Institute for Food and Drug Control, Zhoushan 316012, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1200147899591000519, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147897519014231, authorId=1200147899167375802, language=CN, stringName=严卓彦, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, address=1 舟山市食品药品检验检测研究院,国家海洋食品质量检验检测中心, 浙江 舟山 316012, bio={"content":"

严卓彦,女,硕士 研究方向:药品微生物检测研究 Tel:(0580)2187150

"}, bioImg=null, bioContent=

严卓彦,女,硕士 研究方向:药品微生物检测研究 Tel:(0580)2187150

, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1200147898932494759, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147897519014231, xref=1, ext=[AuthorCompanyExt(id=1200147898940883368, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147897519014231, companyId=1200147898932494759, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1 National Ocean Food Quality Inspection Center, Zhoushan Institute for Food and Drug Control, Zhoushan 316012, China), AuthorCompanyExt(id=1200147898949271977, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147897519014231, companyId=1200147898932494759, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1 舟山市食品药品检验检测研究院,国家海洋食品质量检验检测中心, 浙江 舟山 316012)])]), Author(id=1200147899721023950, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147897519014231, orderNo=1, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1200147899838464468, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147897519014231, authorId=1200147899721023950, language=EN, stringName=Yuxiang TAN, firstName=Yuxiang, middleName=null, lastName=TAN, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=2, address=2 CAS Key Laboratory of Quantitative Engineering Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1200147899989459422, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147897519014231, authorId=1200147899721023950, language=CN, stringName=谭宇翔, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=2, address=2 中国科学院深圳先进技术研究院,深圳合成生物学创新研究院,中国科学院定量工程生物学重点实验室, 广东 深圳 518055, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1200147899058323887, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147897519014231, xref=2, ext=[AuthorCompanyExt(id=1200147899070906803, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147897519014231, companyId=1200147899058323887, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2 CAS Key Laboratory of Quantitative Engineering Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China), AuthorCompanyExt(id=1200147899083489716, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147897519014231, companyId=1200147899058323887, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2 中国科学院深圳先进技术研究院,深圳合成生物学创新研究院,中国科学院定量工程生物学重点实验室, 广东 深圳 518055)])]), Author(id=1200147900056568291, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147897519014231, orderNo=2, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1200147900144648683, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147897519014231, authorId=1200147900056568291, language=EN, stringName=Jiheng LIN, firstName=Jiheng, middleName=null, lastName=LIN, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, address=1 National Ocean Food Quality Inspection Center, Zhoushan Institute for Food and Drug Control, Zhoushan 316012, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1200147900253700593, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147897519014231, authorId=1200147900056568291, language=CN, stringName=林吉恒, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, address=1 舟山市食品药品检验检测研究院,国家海洋食品质量检验检测中心, 浙江 舟山 316012, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1200147898932494759, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147897519014231, xref=1, ext=[AuthorCompanyExt(id=1200147898940883368, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147897519014231, companyId=1200147898932494759, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1 National Ocean Food Quality Inspection Center, Zhoushan Institute for Food and Drug Control, Zhoushan 316012, China), AuthorCompanyExt(id=1200147898949271977, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147897519014231, companyId=1200147898932494759, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1 舟山市食品药品检验检测研究院,国家海洋食品质量检验检测中心, 浙江 舟山 316012)])]), Author(id=1200147900417278459, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147897519014231, orderNo=3, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1200147900555690495, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147897519014231, authorId=1200147900417278459, language=EN, stringName=Yingying XIA, firstName=Yingying, middleName=null, lastName=XIA, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, address=1 National Ocean Food Quality Inspection Center, Zhoushan Institute for Food and Drug Control, Zhoushan 316012, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1200147900752822787, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147897519014231, authorId=1200147900417278459, language=CN, stringName=夏瑛瑛, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, address=1 舟山市食品药品检验检测研究院,国家海洋食品质量检验检测中心, 浙江 舟山 316012, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1200147898932494759, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147897519014231, xref=1, ext=[AuthorCompanyExt(id=1200147898940883368, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147897519014231, companyId=1200147898932494759, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1 National Ocean Food Quality Inspection Center, Zhoushan Institute for Food and Drug Control, Zhoushan 316012, China), AuthorCompanyExt(id=1200147898949271977, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147897519014231, companyId=1200147898932494759, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1 舟山市食品药品检验检测研究院,国家海洋食品质量检验检测中心, 浙江 舟山 316012)])]), Author(id=1200147900849291785, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147897519014231, orderNo=4, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1200147900924789263, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147897519014231, authorId=1200147900849291785, language=EN, stringName=Ying SUN, firstName=Ying, middleName=null, lastName=SUN, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, address=1 National Ocean Food Quality Inspection Center, Zhoushan Institute for Food and Drug Control, Zhoushan 316012, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1200147901033841172, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147897519014231, authorId=1200147900849291785, language=CN, stringName=孙瑛, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, address=1 舟山市食品药品检验检测研究院,国家海洋食品质量检验检测中心, 浙江 舟山 316012, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1200147898932494759, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147897519014231, xref=1, ext=[AuthorCompanyExt(id=1200147898940883368, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147897519014231, companyId=1200147898932494759, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1 National Ocean Food Quality Inspection Center, Zhoushan Institute for Food and Drug Control, Zhoushan 316012, China), AuthorCompanyExt(id=1200147898949271977, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147897519014231, companyId=1200147898932494759, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1 舟山市食品药品检验检测研究院,国家海洋食品质量检验检测中心, 浙江 舟山 316012)])]), Author(id=1200147901155475994, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147897519014231, orderNo=5, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1200147901306470948, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147897519014231, authorId=1200147901155475994, language=EN, stringName=Jie CUI, firstName=Jie, middleName=null, lastName=CUI, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, address=1 National Ocean Food Quality Inspection Center, Zhoushan Institute for Food and Drug Control, Zhoushan 316012, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1200147901423911469, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147897519014231, authorId=1200147901155475994, language=CN, stringName=崔洁, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, address=1 舟山市食品药品检验检测研究院,国家海洋食品质量检验检测中心, 浙江 舟山 316012, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1200147898932494759, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147897519014231, xref=1, ext=[AuthorCompanyExt(id=1200147898940883368, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147897519014231, companyId=1200147898932494759, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1 National Ocean Food Quality Inspection Center, Zhoushan Institute for Food and Drug Control, Zhoushan 316012, China), AuthorCompanyExt(id=1200147898949271977, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147897519014231, companyId=1200147898932494759, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1 舟山市食品药品检验检测研究院,国家海洋食品质量检验检测中心, 浙江 舟山 316012)])]), Author(id=1200147901562323503, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147897519014231, orderNo=6, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1200147901679764021, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147897519014231, authorId=1200147901562323503, language=EN, stringName=Zhuliang HUANG, firstName=Zhuliang, middleName=null, lastName=HUANG, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, address=1 National Ocean Food Quality Inspection Center, Zhoushan Institute for Food and Drug Control, Zhoushan 316012, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1200147901784621628, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147897519014231, authorId=1200147901562323503, language=CN, stringName=黄朱梁, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, address=1 舟山市食品药品检验检测研究院,国家海洋食品质量检验检测中心, 浙江 舟山 316012, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1200147898932494759, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147897519014231, xref=1, ext=[AuthorCompanyExt(id=1200147898940883368, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147897519014231, companyId=1200147898932494759, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1 National Ocean Food Quality Inspection Center, Zhoushan Institute for Food and Drug Control, Zhoushan 316012, China), AuthorCompanyExt(id=1200147898949271977, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147897519014231, companyId=1200147898932494759, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1 舟山市食品药品检验检测研究院,国家海洋食品质量检验检测中心, 浙江 舟山 316012)])])], keywords=[Keyword(id=1200147901914645059, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147897519014231, language=EN, orderNo=1, keyword=Metagenomic sequencing), Keyword(id=1200147902011114056, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147897519014231, language=EN, orderNo=2, keyword=Bacillus cereus specific PCR), Keyword(id=1200147902120165967, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147897519014231, language=EN, orderNo=3, keyword=MLST), Keyword(id=1200147902225023569, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147897519014231, language=EN, orderNo=4, keyword=drug contamination), Keyword(id=1200147902338269780, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147897519014231, language=EN, orderNo=5, keyword=bacteria identification), Keyword(id=1200147902434738778, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147897519014231, language=EN, orderNo=6, keyword=bacteria tracing), Keyword(id=1200147902556373602, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147897519014231, language=CN, orderNo=1, keyword=宏基因组测序), Keyword(id=1200147902652842597, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147897519014231, language=CN, orderNo=2, keyword=蜡样芽孢杆菌特异性PCR), Keyword(id=1200147902724145769, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147897519014231, language=CN, orderNo=3, keyword=多位点序列分型), Keyword(id=1200147902929666668, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147897519014231, language=CN, orderNo=4, keyword=药品污染), Keyword(id=1200147903026135663, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147897519014231, language=CN, orderNo=5, keyword=细菌鉴定), Keyword(id=1200147903160353395, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147897519014231, language=CN, orderNo=6, keyword=细菌溯源)], refs=[Reference(id=1200147905580466879, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147897519014231, doi=null, pmid=null, pmcid=null, year=2021, volume=38, issue=null, pageStart=1870, pageEnd=1877, url=null, language=null, rfNumber=[1], rfOrder=0, authorNames=WU Y W, WANG Y, CHE Y, journalName=Chin J Mod Appl Pharm(中国现代应用药学), refType=null, unstructuredReference=WU Y W, WANG Y, CHE Y, et al. Review and Analysis of International Micobial Contamination-related Drug recalls from 2013 to 2019[J]. Chin J Mod Appl Pharm(中国现代应用药学), 2021, 38: 1870-1877., articleTitle=Review and Analysis of International Micobial Contamination-related Drug recalls from 2013 to 2019, refAbstract=null), Reference(id=1200147905668547266, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147897519014231, doi=null, pmid=null, pmcid=null, year=2007, volume=104, issue=29, pageStart=11889, pageEnd=11894, url=null, language=null, rfNumber=[2], rfOrder=1, authorNames=MARCY Y, OUVERNEY C, BIK E M, journalName=Proc Natl Acad Sci USA, refType=null, unstructuredReference=MARCY Y, OUVERNEY C, BIK E M, et al. Dissecting biological “dark matter” with single-cell genetic analysis of rare and uncultivated TM7 microbes from the human mouth[J]. Proc Natl Acad Sci USA, 2007, 104(29): 11889-11894., articleTitle=Dissecting biological “dark matter” with single-cell genetic analysis of rare and uncultivated TM7 microbes from the human mouth, refAbstract=null), Reference(id=1200147905760821957, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147897519014231, doi=null, pmid=null, pmcid=null, year=2019, volume=568, issue=7753, pageStart=505, pageEnd=510, url=null, language=null, rfNumber=[3], rfOrder=2, authorNames=NAYFACH S, SHI Z J, SESHADRI R, journalName=Nature, refType=null, unstructuredReference=NAYFACH S, SHI Z J, SESHADRI R, et al. New insights from uncultivated genomes of the global human gut microbiome[J]. Nature, 2019, 568(7753): 505-510., articleTitle=New insights from uncultivated genomes of the global human gut microbiome, refAbstract=null), Reference(id=1200147905853096648, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147897519014231, doi=null, pmid=null, pmcid=null, year=2020, volume=11, issue=null, pageStart=1567, pageEnd=null, url=null, language=null, rfNumber=[4], rfOrder=3, authorNames=CYCIL L M, DASSARMA S, PECHER W, journalName=Front Microbiol, refType=null, unstructuredReference=CYCIL L M, DASSARMA S, PECHER W, et al. Metagenomic insights into the diversity of halophilic microorganisms indigenous to the Karak salt mine, Pakistan[J]. Front Microbiol, 2020, 11: 1567., articleTitle=Metagenomic insights into the diversity of halophilic microorganisms indigenous to the Karak salt mine, Pakistan, refAbstract=null), Reference(id=1200147905916011211, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147897519014231, doi=null, pmid=null, pmcid=null, year=2022, volume=10, issue=1, pageStart=e0199421, pageEnd=null, url=null, language=null, rfNumber=[5], rfOrder=4, authorNames=LOMBARDINO J, BIJLANI S, SINGH N K, journalName=Microbiol Spectr, refType=null, unstructuredReference=LOMBARDINO J, BIJLANI S, SINGH N K, et al. Genomic characterization of potential plant growth-promoting features of Sphingomonas strains isolated from the international space station[J]. Microbiol Spectr, 2022, 10(1): e0199421. DOI: 10.1128/spectrum.01994-21., articleTitle=Genomic characterization of potential plant growth-promoting features of Sphingomonas strains isolated from the international space station, refAbstract=null), Reference(id=1200147905995702988, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147897519014231, doi=null, pmid=null, pmcid=null, year=2012, volume=3, issue=1, pageStart=1245, pageEnd=null, url=null, language=null, rfNumber=[6], rfOrder=5, authorNames=KARLSSON FH, FAK F, NOOKAEW I, journalName=Nat Commun, refType=null, unstructuredReference=KARLSSON FH, FAK F, NOOKAEW I, et al. Symptomatic atherosclerosis is associated with an altered gut metagenome[J]. Nat Commun, 2012, 3(1): 1245. DOI: 10.1038/ncomms2266., articleTitle=Symptomatic atherosclerosis is associated with an altered gut metagenome, refAbstract=null), Reference(id=1200147906100560593, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147897519014231, doi=null, pmid=null, pmcid=null, year=2022, volume=37, issue=null, pageStart=1408, pageEnd=1412, url=null, language=null, rfNumber=[7], rfOrder=6, authorNames=QIAN X W, WU Y R, HAO T Y, journalName=Dis Surveill(疾病监测), refType=null, unstructuredReference=QIAN X W, WU Y R, HAO T Y, et al. Strain-level bacterial analysis from shotgun metagenomics: a review[J]. Dis Surveill(疾病监测), 2022, 37: 1408-1412., articleTitle=Strain-level bacterial analysis from shotgun metagenomics: a review, refAbstract=null), Reference(id=1200147906243166932, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147897519014231, doi=null, pmid=null, pmcid=null, year=2021, volume=7, issue=4, pageStart=000547, pageEnd=null, url=null, language=null, rfNumber=[8], rfOrder=7, authorNames=BUYTAERS F E, SALTYKOVA A, MATTHEUS W, journalName=Microb Genom, refType=null, unstructuredReference=BUYTAERS F E, SALTYKOVA A, MATTHEUS W, et al. Application of a strain-level shotgun metagenomics approach on food samples: resolution of the source of a Salmonella food-borne outbreak[J]. Microb Genom, 2021, 7(4): 000547. DOI: 10.1099/mgen.0.000547., articleTitle=Application of a strain-level shotgun metagenomics approach on food samples: resolution of the source of a Salmonella food-borne outbreak, refAbstract=null), Reference(id=1200147906381578967, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147897519014231, doi=null, pmid=null, pmcid=null, year=2019, volume=4, issue=1, pageStart=e00529, pageEnd=18, url=null, language=null, rfNumber=[9], rfOrder=8, authorNames=MU A, KWONG J C, ISLES N S, journalName=mSphere, refType=null, unstructuredReference=MU A, KWONG J C, ISLES N S, et al. Reconstruction of the genomes of drug-resistant pathogens for outbreak investigation through metagenomic sequencing[J]. mSphere, 2019, 4(1): e00529-18. DOI: 10.1128/mSphere.00529-18., articleTitle=Reconstruction of the genomes of drug-resistant pathogens for outbreak investigation through metagenomic sequencing, refAbstract=null), Reference(id=1200147906499019483, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147897519014231, doi=null, pmid=null, pmcid=null, year=2020, volume=36, issue=12, pageStart=2610, pageEnd=2621, url=null, language=null, rfNumber=[10], rfOrder=9, authorNames=TAN Y X, HU H, LI C H, journalName=Chin J Biotech(生物工程学报), refType=null, unstructuredReference=TAN Y X, HU H, LI C H, et al. Research progress and applications of strain analysis based on metagenomic data[J]. Chin J Biotech(生物工程学报), 2020, 36(12): 2610-2621., articleTitle=Research progress and applications of strain analysis based on metagenomic data, refAbstract=null), Reference(id=1200147906620654301, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147897519014231, doi=null, pmid=null, pmcid=null, year=2015, volume=12, issue=10, pageStart=902, pageEnd=903, url=null, language=null, rfNumber=[11], rfOrder=10, authorNames=TRUONG D T, FRANZOSA E A, TICKLE T L, journalName=Nat Methods, refType=null, unstructuredReference=TRUONG D T, FRANZOSA E A, TICKLE T L, et al. MetaPhlAn2 for enhanced metagenomic taxonomic profiling[J]. Nat Methods, 2015, 12(10): 902-903., articleTitle=MetaPhlAn2 for enhanced metagenomic taxonomic profiling, refAbstract=null), Reference(id=1200147906784232159, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147897519014231, doi=null, pmid=null, pmcid=null, year=2017, volume=27, issue=4, pageStart=626, pageEnd=638, url=null, language=null, rfNumber=[12], rfOrder=11, authorNames=TRUONG D T, TETT A, PASOLLI E, journalName=Genome Res, refType=null, unstructuredReference=TRUONG D T, TETT A, PASOLLI E, et al. Microbial strain-level population structure and genetic diversity from metagenomes[J]. Genome Res, 2017, 27(4): 626-638., articleTitle=Microbial strain-level population structure and genetic diversity from metagenomes, refAbstract=null), Reference(id=1200147906918449891, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147897519014231, doi=null, pmid=null, pmcid=null, year=2018, volume=24, issue=12, pageStart=1809, pageEnd=1814, url=null, language=null, rfNumber=[13], rfOrder=12, authorNames=TAMBURINI F B, ANDERMANN T M, TKACHENKO E, journalName=Nat Med, refType=null, unstructuredReference=TAMBURINI F B, ANDERMANN T M, TKACHENKO E, et al. Precision identification of diverse bloodstream pathogens in the gut microbiome[J]. Nat Med, 2018, 24(12): 1809-1814., articleTitle=Precision identification of diverse bloodstream pathogens in the gut microbiome, refAbstract=null), Reference(id=1200147907010724583, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147897519014231, doi=null, pmid=null, pmcid=null, year=2022, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[14], rfOrder=13, authorNames=HU H, TAN Y X, LI C H, journalName=iMeta, refType=null, unstructuredReference=HU H, TAN Y X, LI C H, et al. StrainPanDA: linked reconstruction of strain composition and gene content profiles via pangenome-based decomposition of metagenomic data[J]. iMeta, 2022. https://doi.org/10.1002/imt2.41., articleTitle=StrainPanDA: linked reconstruction of strain composition and gene content profiles via pangenome-based decomposition of metagenomic data, refAbstract=null), Reference(id=1200147907186885353, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147897519014231, doi=null, pmid=null, pmcid=null, year=2016, volume=13, issue=5, pageStart=435, pageEnd=438, url=null, language=null, rfNumber=[15], rfOrder=14, authorNames=SCHOLZ M, WARD D V, PASOLLI E, journalName=Nat Methods, refType=null, unstructuredReference=SCHOLZ M, WARD D V, PASOLLI E, et al. Strain-level microbial epidemiology and population genomics from shotgun metagenomics[J]. Nat Methods, 2016, 13(5): 435-438., articleTitle=Strain-level microbial epidemiology and population genomics from shotgun metagenomics, refAbstract=null), Reference(id=1200147907266577133, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147897519014231, doi=null, pmid=null, pmcid=null, year=2022, volume=34, issue=1, pageStart=128, pageEnd=140, url=null, language=null, rfNumber=[16], rfOrder=15, authorNames=YAN J T, QIAO K, CAI Y F, journalName=Acta Agric Zhejiangensis(浙江农业学报), refType=null, unstructuredReference=YAN J T, QIAO K, CAI Y F. Application of rpoB, gyrA and cheA genes in identifying Bacillus genus[J]. Acta Agric Zhejiangensis(浙江农业学报), 2022, 34(1): 128-140, articleTitle=Application of rpoB, gyrA and cheA genes in identifying Bacillus genus, refAbstract=null), Reference(id=1200147907337880303, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147897519014231, doi=null, pmid=null, pmcid=null, year=1996, volume=62, issue=4, pageStart=1369, pageEnd=1377, url=null, language=null, rfNumber=[17], rfOrder=16, authorNames=KUO W S, CHAK K F, journalName=Appl Environ Microbiol, refType=null, unstructuredReference=KUO W S, CHAK K F. Identification of novel cry-type genes from Bacillus thuringiensis stains on the basis of restriction fragment length polymorphism of the PCR-amplified DNA[J]. Appl Environ Microbiol, 1996, 62(4): 1369-1377., articleTitle=Identification of novel cry-type genes from Bacillus thuringiensis stains on the basis of restriction fragment length polymorphism of the PCR-amplified DNA, refAbstract=null), Reference(id=1200147907446932212, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147897519014231, doi=null, pmid=null, pmcid=null, year=2021, volume=48, issue=4, pageStart=1360, pageEnd=1372, url=null, language=null, rfNumber=[18], rfOrder=17, authorNames=ZHANG L Y, SONG L L, JIA W J, journalName=Microbiol China(微生物学通报), refType=null, unstructuredReference=ZHANG L Y, SONG L L, JIA W J, et al. Research process on detection methods of Bacillus cereus[J]. Microbiol China(微生物学通报), 2021, 48(4): 1360-1372., articleTitle=Research process on detection methods of Bacillus cereus, refAbstract=null), Reference(id=1200147907551789816, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147897519014231, doi=null, pmid=null, pmcid=null, year=2018, volume=45, issue=3, pageStart=635, pageEnd=642, url=null, language=null, rfNumber=[19], rfOrder=18, authorNames=HUANG J J, LUO J, HE H X, journalName=China Anim Husb Vet Med(中国畜牧兽医), refType=null, unstructuredReference=HUANG J J, LUO J, HE H X. Research progress on detection methods for Bacillus cereus[J]. China Anim Husb Vet Med(中国畜牧兽医), 2018, 45(3): 635-642., articleTitle=Research progress on detection methods for Bacillus cereus, refAbstract=null), Reference(id=1200147907669230333, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147897519014231, doi=null, pmid=null, pmcid=null, year=2018, volume=39, issue=1, pageStart=74, pageEnd=83, url=null, language=null, rfNumber=[20], rfOrder=19, authorNames=LU J Q, LI S C, WANG D H, journalName=J Henan Univ Sci Technol Nat Sci(河南科技大学学报自然科学版), refType=null, unstructuredReference=LU J Q, LI S C, WANG D H, et al. Combined 16S rDNA and gyrB gene sequence to identify a strain of Bacillus cereus[J]. J Henan Univ Sci Technol Nat Sci(河南科技大学学报自然科学版), 2018, 39(1): 74-83., articleTitle=Combined 16S rDNA and gyrB gene sequence to identify a strain of Bacillus cereus, refAbstract=null), Reference(id=1200147907782476545, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147897519014231, doi=null, pmid=null, pmcid=null, year=2012, volume=9, issue=9, pageStart=777, pageEnd=785, url=null, language=null, rfNumber=[21], rfOrder=20, authorNames=VERA A, PABLO S F, MARIA R C, journalName=Foodborne Pathog Dis, refType=null, unstructuredReference=VERA A, PABLO S F, MARIA R C, et al. Development of a high-resolution melting-based approach for efficient differentiation among Bacillus cereus group isolates[J]. Foodborne Pathog Dis, 2012, 9(9): 777-785., articleTitle=Development of a high-resolution melting-based approach for efficient differentiation among Bacillus cereus group isolates, refAbstract=null), Reference(id=1200147907929277189, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147897519014231, doi=null, pmid=null, pmcid=null, year=2002, volume=92, issue=null, pageStart=912, pageEnd=919, url=null, language=null, rfNumber=[22], rfOrder=21, authorNames=CHEN M L, TSEN H Y, journalName=J Appl Microbiol, refType=null, unstructuredReference=CHEN M L, TSEN H Y. Discrimination of Bacillus cereus and Bacillus thuringiensis with 16S rRNA and gyrB gene based PCR primers and sequencing of their annealing sites[J]. J Appl Microbiol, 2002, 92: 912-919., articleTitle=Discrimination of Bacillus cereus and Bacillus thuringiensis with 16S rRNA and gyrB gene based PCR primers and sequencing of their annealing sites, refAbstract=null), Reference(id=1200147908050912007, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147897519014231, doi=null, pmid=null, pmcid=null, year=2004, volume=24, issue=10, pageStart=49, pageEnd=null, url=null, language=null, rfNumber=[23], rfOrder=22, authorNames=MEI L, journalName=Clin Med(临床医学), refType=null, unstructuredReference=MEI L. Condition the etiology of diarrhea caused by pathogenic bacteria[J]. Clin Med(临床医学), 2004, 24(10): 49., articleTitle=Condition the etiology of diarrhea caused by pathogenic bacteria, refAbstract=null), Reference(id=1200147908180935434, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147897519014231, doi=null, pmid=null, pmcid=null, year=2012, volume=15, issue=6, pageStart=1, pageEnd=13, url=null, language=null, rfNumber=[24], rfOrder=23, authorNames=SUTLON S, journalName=Am Pharm Rev, refType=null, unstructuredReference=SUTLON S. What is an “objectionable organism”?[J]. Am Pharm Rev, 2012, 15(6): 1-13., articleTitle=What is an “objectionable organism”?, refAbstract=null), Reference(id=1200147908273210124, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147897519014231, doi=null, pmid=null, pmcid=null, year=2019, volume=null, issue=null, pageStart=371, pageEnd=null, url=null, language=null, rfNumber=[25], rfOrder=24, authorNames=ROESTI D, GOVERDE M, journalName=Pharmaceutical Microbiological Quality Assurance and Control, refType=null, unstructuredReference=ROESTI D, GOVERDE M. Pharmaceutical Microbiological Quality Assurance and Control[M]. New Jersey: John Wiley & Sons, Inc., 2019: 371-400., articleTitle=null, refAbstract=null)], funds=[Fund(id=1200147905416889019, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147897519014231, awardId=2023010, language=CN, fundingSource=浙江省药监局科技计划项目资助(2023010), fundOrder=null, country=null)], companyList=[AuthorCompany(id=1200147898932494759, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147897519014231, xref=1, ext=[AuthorCompanyExt(id=1200147898940883368, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147897519014231, companyId=1200147898932494759, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1 National Ocean Food Quality Inspection Center, Zhoushan Institute for Food and Drug Control, Zhoushan 316012, China), AuthorCompanyExt(id=1200147898949271977, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147897519014231, companyId=1200147898932494759, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1 舟山市食品药品检验检测研究院,国家海洋食品质量检验检测中心, 浙江 舟山 316012)]), AuthorCompany(id=1200147899058323887, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147897519014231, xref=2, ext=[AuthorCompanyExt(id=1200147899070906803, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147897519014231, companyId=1200147899058323887, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2 CAS Key Laboratory of Quantitative Engineering Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China), AuthorCompanyExt(id=1200147899083489716, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147897519014231, companyId=1200147899058323887, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2 中国科学院深圳先进技术研究院,深圳合成生物学创新研究院,中国科学院定量工程生物学重点实验室, 广东 深圳 518055)])], figs=[ArticleFig(id=1200147903290376825, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147897519014231, language=EN, label=Fig.1, caption=NJ trees based on panC sequences of five strains and Bacillus cereus, Bacillus thuringiensis, Bacillus mycoides, Bacillus pacificus, Bacillus wiedmannii from NCBI, figureFileSmall=NEiZD+6yxS/uY2dK/543cw==, figureFileBig=SKv1SwBcWOiGK0EnLnnnlA==, tableContent=null), ArticleFig(id=1200147903365874303, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147897519014231, language=CN, label=图1, caption=基于5个菌株和NCBI的蜡样芽孢杆菌、苏云金芽孢杆菌、蕈状芽孢杆菌、太平洋芽孢杆菌、魏德曼芽孢杆菌的panC基因序列的NJ进化树, figureFileSmall=NEiZD+6yxS/uY2dK/543cw==, figureFileBig=SKv1SwBcWOiGK0EnLnnnlA==, tableContent=null), ArticleFig(id=1200147903604949634, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147897519014231, language=EN, label=Fig.2, caption=NJ trees based on gyrA sequences of five strains and Bacillus cereus, Bacillus thuringiensis, Bacillus mycoides, Bacillus pacificus, Bacillus wiedmannii from NCBI, figureFileSmall=c46w0dlSfNa+4O9WKHN4Hw==, figureFileBig=9zfcvw1tnC7bxmcDijJh3A==, tableContent=null), ArticleFig(id=1200147903722390150, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147897519014231, language=CN, label=图2, caption=基于5个菌株和NCBI的蜡样芽孢杆菌、苏云金芽孢杆菌、蕈状芽孢杆菌、太平洋芽孢杆菌、魏德曼芽孢杆菌的gyrA基因序列的NJ进化树, figureFileSmall=c46w0dlSfNa+4O9WKHN4Hw==, figureFileBig=9zfcvw1tnC7bxmcDijJh3A==, tableContent=null), ArticleFig(id=1200147903923716746, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147897519014231, language=EN, label=Tab.1, caption=

Specific PCR primer pairs for Bacillus cereus

, figureFileSmall=null, figureFileBig=null, tableContent=
Gene Primer
sequences		 PCR
products/bp		 Reference
gyrA F:GCGTCTGCAACGTTTAACTGG
R:TGTCGCTACCTCTTGCTCATC		 1 084 [14]
panC F:TGGCCCAAATGAAGAT
R:TAACTGCAATAGCTAAAATGAT		 600 Designed by
this research
cry F:AGGACCAGGATTTACAGGAGG
R:GCTGTGACACGAAGGATATAGCCAC		 1 600~1 700 [15]
), ArticleFig(id=1200147904091488912, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147897519014231, language=CN, label=表1, caption=

蜡样芽孢杆菌群特异性PCR扩增基因及引物

, figureFileSmall=null, figureFileBig=null, tableContent=
Gene Primer
sequences		 PCR
products/bp		 Reference
gyrA F:GCGTCTGCAACGTTTAACTGG
R:TGTCGCTACCTCTTGCTCATC		 1 084 [14]
panC F:TGGCCCAAATGAAGAT
R:TAACTGCAATAGCTAAAATGAT		 600 Designed by
this research
cry F:AGGACCAGGATTTACAGGAGG
R:GCTGTGACACGAAGGATATAGCCAC		 1 600~1 700 [15]
), ArticleFig(id=1200147904225706643, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147897519014231, language=EN, label=Tab.2, caption=

Primer pairs and PCR conditions for seven alleles of bacillus cereus

, figureFileSmall=null, figureFileBig=null, tableContent=
Gene Primer sequences Annealing temperature/℃
glpF F:GCG TTT GTG CTG GTG TAA GT
R:CTG CAA TCG GAA GGA AGA AG		 59
gmk F:ATT TAA GTG AGG AAG GGT AGG
R:GCA ATG TTC ACC AAC CAC AA		 56
ilvD F:CGG GGC AAA CAT TAA GAG AA
R:GGT TCT GGT CGT TTC CAT TC		 58
pta F:GCA GAG CGT TTA GCA AAA GAA
R:TGC AAT GCG AGT TGC TTC TA		 56
pycA F:GCG TTA GGT GGA AAC GAA AG
R:CGC GTC CAA GTT TAT GGA AT		 57
tpi F:GCC CAG TAG CAC TTA GCG AC
R:CCG AAA CCG TCA AGA ATG AT		 58
pur F:CTG CTG CGA AAA ATC ACA AA
R:CTC ACG ATT CGC TGC AAT AA		 56
), ArticleFig(id=1200147904334758552, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147897519014231, language=CN, label=表2, caption=

蜡样芽孢杆菌7个管家基因PCR扩增引物及条件

, figureFileSmall=null, figureFileBig=null, tableContent=
Gene Primer sequences Annealing temperature/℃
glpF F:GCG TTT GTG CTG GTG TAA GT
R:CTG CAA TCG GAA GGA AGA AG		 59
gmk F:ATT TAA GTG AGG AAG GGT AGG
R:GCA ATG TTC ACC AAC CAC AA		 56
ilvD F:CGG GGC AAA CAT TAA GAG AA
R:GGT TCT GGT CGT TTC CAT TC		 58
pta F:GCA GAG CGT TTA GCA AAA GAA
R:TGC AAT GCG AGT TGC TTC TA		 56
pycA F:GCG TTA GGT GGA AAC GAA AG
R:CGC GTC CAA GTT TAT GGA AT		 57
tpi F:GCC CAG TAG CAC TTA GCG AC
R:CCG AAA CCG TCA AGA ATG AT		 58
pur F:CTG CTG CGA AAA ATC ACA AA
R:CTC ACG ATT CGC TGC AAT AA		 56
), ArticleFig(id=1200147904443810459, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147897519014231, language=EN, label=Tab.3, caption=

The strain-level analysis results of four samples by MetaPhlan

, figureFileSmall=null, figureFileBig=null, tableContent=
Sample Genus(%) Species(%) SGB(%)
B18 Bacillus(98.9 Bacillus cereus(98.5) Bacillus cereus SGB7697(98.3)
Priestia(1.0) Priestia megaterium(1.0) Priestia megaterium SGB7678 (1.0)
B20 Bacillus(99.4) Bacillus cereus(99.4) Bacillus cereus SGB7703 (94.4)
Bacillus cereus SGB7697(4.9)
HJ1 Acinetobacter(100) Acinetobacter radioresistens(100) Acinetobacter radioresistens SGB10375 (100)
HJ2 Bacillus(56.9) Bacillus cereus(56.9) Bacillus cereus SGB7697 (56.9)
Acinetobacter(38.6) Acinetobacter johnsonii(38.6) Acinetobacter johnsonii SGB10398 (38.6)
Pseudomonas(2.9) Pseudomonas oryzihabitans(2.9) Pseudomonas oryzihabitans SGB12309(2.9)
Niallia(1.6) Niallia taxi(1.6) Niallia taxi SGB30277(1.6)
), ArticleFig(id=1200147904586416799, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147897519014231, language=CN, label=表3, caption=

4个样本宏基因组测序菌株水平分析的MetaPhlAn结果

, figureFileSmall=null, figureFileBig=null, tableContent=
Sample Genus(%) Species(%) SGB(%)
B18 Bacillus(98.9 Bacillus cereus(98.5) Bacillus cereus SGB7697(98.3)
Priestia(1.0) Priestia megaterium(1.0) Priestia megaterium SGB7678 (1.0)
B20 Bacillus(99.4) Bacillus cereus(99.4) Bacillus cereus SGB7703 (94.4)
Bacillus cereus SGB7697(4.9)
HJ1 Acinetobacter(100) Acinetobacter radioresistens(100) Acinetobacter radioresistens SGB10375 (100)
HJ2 Bacillus(56.9) Bacillus cereus(56.9) Bacillus cereus SGB7697 (56.9)
Acinetobacter(38.6) Acinetobacter johnsonii(38.6) Acinetobacter johnsonii SGB10398 (38.6)
Pseudomonas(2.9) Pseudomonas oryzihabitans(2.9) Pseudomonas oryzihabitans SGB12309(2.9)
Niallia(1.6) Niallia taxi(1.6) Niallia taxi SGB30277(1.6)
), ArticleFig(id=1200147904741606051, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147897519014231, language=EN, label=Tab.4, caption=

The strain-level analysis results of four samples by StrainSifter

, figureFileSmall=null, figureFileBig=null, tableContent=
Samples
compared		 SNVs Bases
compared		 Diversity
ratio/%
B18 to HJ2 48 772 4 546 132 1.07
B20 to HJ2 65 139 4 066 579 1.60
B18 to B20 77 754 4 141 063 1.88
), ArticleFig(id=1200147904871629477, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147897519014231, language=CN, label=表4, caption=

4个样本宏基因组测序菌株水平分析的StraniSifter结果

, figureFileSmall=null, figureFileBig=null, tableContent=
Samples
compared		 SNVs Bases
compared		 Diversity
ratio/%
B18 to HJ2 48 772 4 546 132 1.07
B20 to HJ2 65 139 4 066 579 1.60
B18 to B20 77 754 4 141 063 1.88
), ArticleFig(id=1200147904968098474, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147897519014231, language=EN, label=Tab.5, caption=

Sequencing results of 16srRNA and specific PCR products for the five cultured strains

, figureFileSmall=null, figureFileBig=null, tableContent=
Sample The BLAST result of
16srDNA (identity/%)		 The BLAST result of specific PCR product (identity/%)
gyrA PanC cry
B18 Bacillus proteolyticus/sanguinis(99.93) Bacillus cereus/thuringiensis(>99.5) Bacillus cereus(>99.5) No PCR product
B20 Bacillus tropicus/nitratireducens/luti/albus/paramycoides(99.66) Bacillus cereus(99.81) Bacillus cereus/pacificus(>99) No PCR product
HJ-1 Bacillus cereus(99.86) Bacillus cereus/thuringiensis(>99.5) Bacillus cereus/thuringiensis(>99) No PCR product
HJ-2 Bacillus proteolyticus/sanguinis/wiedmannii/cereus(>99.7) Bacillus cereus/thuringiensis(>99.5) Bacillus cereus/thuringiensis(>99.5) No PCR product
HJ-3 Bacillus toyonensis/thuringiensis(99.47) Bacillus cereus/thuringiensis(>99.5) Bacillus cereus/thuringiensis(>99.5) No PCR product
), ArticleFig(id=1200147905047790253, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147897519014231, language=CN, label=表5, caption=

培养法分离得到5株细菌的16srRNA全长测序、芽孢杆菌特异性PCR鉴定结果

, figureFileSmall=null, figureFileBig=null, tableContent=
Sample The BLAST result of
16srDNA (identity/%)		 The BLAST result of specific PCR product (identity/%)
gyrA PanC cry
B18 Bacillus proteolyticus/sanguinis(99.93) Bacillus cereus/thuringiensis(>99.5) Bacillus cereus(>99.5) No PCR product
B20 Bacillus tropicus/nitratireducens/luti/albus/paramycoides(99.66) Bacillus cereus(99.81) Bacillus cereus/pacificus(>99) No PCR product
HJ-1 Bacillus cereus(99.86) Bacillus cereus/thuringiensis(>99.5) Bacillus cereus/thuringiensis(>99) No PCR product
HJ-2 Bacillus proteolyticus/sanguinis/wiedmannii/cereus(>99.7) Bacillus cereus/thuringiensis(>99.5) Bacillus cereus/thuringiensis(>99.5) No PCR product
HJ-3 Bacillus toyonensis/thuringiensis(99.47) Bacillus cereus/thuringiensis(>99.5) Bacillus cereus/thuringiensis(>99.5) No PCR product
), ArticleFig(id=1200147905186202292, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147897519014231, language=EN, label=Tab.6, caption=

Multilocus sequence typing(MLST) results of five cultured strains

, figureFileSmall=null, figureFileBig=null, tableContent=
Strain Allele Allelic
profile		 BURST
glpF ilvD pta purH pycA gmk tpiA
B18 14 36 10 2 17 8 10 3 178 Group 1
B20 6 41 5 43 46 4 3 90 singleton
HJ-1 13 9 11 9 12 8 53 2 573 singleton
HJ-2 33 36 30 219 17 8 53 3 179 Group 1
HJ-3 93 170 8 4 39 67 21 3 180 singleton
), ArticleFig(id=1200147905278476982, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147897519014231, language=CN, label=表6, caption=

5个分离菌株的多位点序列分型(MLST)法分型结果

, figureFileSmall=null, figureFileBig=null, tableContent=
Strain Allele Allelic
profile		 BURST
glpF ilvD pta purH pycA gmk tpiA
B18 14 36 10 2 17 8 10 3 178 Group 1
B20 6 41 5 43 46 4 3 90 singleton
HJ-1 13 9 11 9 12 8 53 2 573 singleton
HJ-2 33 36 30 219 17 8 53 3 179 Group 1
HJ-3 93 170 8 4 39 67 21 3 180 singleton
)], attaches=null, journal=Journal(id=1190317596361715715, delFlag=0, nameCn=中国药学杂志, nameEn=Chinese Pharmaceutical Journal, nameHistory1=null, nameHistory2=null, issn=1001-2494, eissn=null, cn=11-2162/R, coden=null, periodic=3, language=CN, oaType=null, ccby=null, superviseOffice=null, ownerOffice=null, pubOffice=null, editorOffice=null, officeType=null, aims=null, clcCode=null, officeProv=null, officeCity=null, officeAddr=null, officeZip=null, officeEmail=null, officePhone=null, editDirector=null, officeDirector=null, officeDirectorPhone=null, officeStaffNum=null, officeEmpNum=null, coverPicUrl=hRN1R6HnoNwYkve/JRn0DA==, journalPrice=null, startedYear=null, abbrevIsoEn=null, journalRemark=null, publicationField=null, createdTime=1761723430007, updatedTime=1761735858241, createdBy=18614031015, updatedBy=13701087609, firstLetterCn=C, firstLetterEn=C, subjectCode=Life Sciences, subjectName=Life Sciences, subjectCodeEn=Life Sciences, subjectNameEn=null, picCn=hRN1R6HnoNwYkve/JRn0DA==, picEn=xSRntM4yOh2wVIE2w+OjYg==, jcr=null, cjcr=null, exts=[JournalExt(id=1190369724262355196, language=CN, name=中国药学杂志, nameHistory1=null, nameHistory2=null, managedBy=, sponsoredBy=, publishedBy=, editorOffice=, officeProv=null, officeCity=null, officeAddr=, officeZip=, editDirector=, officeDirector=null, officePhone=null, coverPicUrl=null, journalRemark=, submitArticleUrl=null, websiteUrl=, createdTime=1761735858264, updatedTime=1761735858264, createdBy=13701087609, updatedBy=13701087609, submissionGuidelinesUrl=, submissionAuthorUrl=https://zgyxzzauthor.manuscriptcloud.com/login, submissionEditorUrl=https://zgyxzzeditor.manuscriptcloud.com/login, submissionReviewUrl=https://zgyxzzauthor.manuscriptcloud.com/login, submissionCeEditorUrl=, submissionAeEditorUrl=, option={"copyright":""}), JournalExt(id=1190369724358824189, language=EN, name=Chinese Pharmaceutical Journal, nameHistory1=null, nameHistory2=null, managedBy=, sponsoredBy=, publishedBy=, editorOffice=, officeProv=null, officeCity=null, officeAddr=, officeZip=, editDirector=, officeDirector=null, officePhone=null, coverPicUrl=null, journalRemark=, submitArticleUrl=null, websiteUrl=, createdTime=1761735858287, updatedTime=1761735858287, createdBy=13701087609, updatedBy=13701087609, submissionGuidelinesUrl=, submissionAuthorUrl=https://zgyxzzauthor.manuscriptcloud.com/login, submissionEditorUrl=https://zgyxzzeditor.manuscriptcloud.com/login, submissionReviewUrl=https://zgyxzzauthor.manuscriptcloud.com/login, submissionCeEditorUrl=, submissionAeEditorUrl=, option={"copyright":""})], databaseList=null, tenantJournalId=1190317699101192196, websiteList=[Website(id=1190317834875011552, webName=null, webTitle=null, webDomain=null, webCopyrigh=null, webIpcNo=null, seoTitle=null, seoKeywords=null, seoDescription=null, tenantJournalId=null, journalId=1190317699101192196, journalNameCn=null, journalNameEn=null, grayFlag=null, tenantId=1146029695717560320, platformId=null, journalGroupId=null, journalGroupNameCn=null, journalGroupNameEn=null, type=1, domain=https://castjournals.cast.org.cn/joweb/zgyxzz/CN, language=CN, createTime=1761723486870, createBy=18614031015, updateTime=1761723510130, updateBy=18614031015, name=中国药学杂志-中文, tplId=1146099689490845704, title=中国药学杂志, delFlag=0, indexPage=/home, props=[WebsiteProps(id=1190318144041353703, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1190317834875011552, code=articleTextType, value=kx, createTime=1761723560581, updateTime=1761723560581, creator=18614031015, updator=18614031015), WebsiteProps(id=1190318144016187876, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1190317834875011552, code=banner, value=null, createTime=1761723560575, updateTime=1761723560575, creator=18614031015, updator=18614031015), WebsiteProps(id=1190318144062325226, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1190317834875011552, code=grayFlag, value=0, createTime=1761723560586, updateTime=1761723560586, creator=18614031015, updator=18614031015), WebsiteProps(id=1190318144007799267, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1190317834875011552, code=logo, value=https://castjournals.cast.org.cn/joweb/zgyxzz/CN/file/pic?fileId=puyAm9wIHqZks7K8hj8APQ==, createTime=1761723560573, updateTime=1761723560573, creator=18614031015, updator=18614031015), WebsiteProps(id=1190318144074908140, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1190317834875011552, code=minRunFlag, value=0, createTime=1761723560589, updateTime=1761723560589, creator=18614031015, updator=18614031015), WebsiteProps(id=1190318144032965094, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1190317834875011552, code=picServerUrl, value=https://castjournals.cast.org.cn/joweb/zgyxzz/CN/file/pic, createTime=1761723560579, updateTime=1761723560579, creator=18614031015, updator=18614031015), WebsiteProps(id=1190318144070713835, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1190317834875011552, code=silenceFlag, value=0, createTime=1761723560588, updateTime=1761723560588, creator=18614031015, updator=18614031015), WebsiteProps(id=1190318144024576485, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1190317834875011552, code=staticResourcePath, value=https://castjournals.cast.org.cn/joweb/cast_kjdb_cn_619/, createTime=1761723560577, updateTime=1761723560577, creator=18614031015, updator=18614031015), WebsiteProps(id=1190318144049742312, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1190317834875011552, code=themeColor, value=null, createTime=1761723560583, updateTime=1761723560583, creator=18614031015, updator=18614031015), WebsiteProps(id=1190318144053936617, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1190317834875011552, code=themeStyle, value=null, createTime=1761723560584, updateTime=1761723560584, creator=18614031015, updator=18614031015)]), Website(id=1190317834937926113, webName=null, webTitle=null, webDomain=null, webCopyrigh=null, webIpcNo=null, seoTitle=null, seoKeywords=null, seoDescription=null, tenantJournalId=null, journalId=1190317699101192196, journalNameCn=null, journalNameEn=null, grayFlag=null, tenantId=1146029695717560320, platformId=null, journalGroupId=null, journalGroupNameCn=null, journalGroupNameEn=null, type=1, domain=https://castjournals.cast.org.cn/joweb/zgyxzz/EN, language=EN, createTime=1761723486885, createBy=18614031015, updateTime=1761723527689, updateBy=18614031015, name=中国药学杂志-英文, tplId=1146101810881728533, title=Chinese Pharmaceutical Journal, delFlag=0, indexPage=/home, props=[WebsiteProps(id=1190318170478051825, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1190317834937926113, code=articleTextType, value=kx, createTime=1761723566884, updateTime=1761723566884, creator=18614031015, updator=18614031015), WebsiteProps(id=1190318170461274606, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1190317834937926113, code=banner, value=null, createTime=1761723566880, updateTime=1761723566880, creator=18614031015, updator=18614031015), WebsiteProps(id=1190318170494829044, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1190317834937926113, code=grayFlag, value=0, createTime=1761723566888, updateTime=1761723566888, creator=18614031015, updator=18614031015), WebsiteProps(id=1190318170452885997, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1190317834937926113, code=logo, value=https://castjournals.cast.org.cn/joweb/zgyxzz/EN/file/pic?fileId=puyAm9wIHqZks7K8hj8APQ==, createTime=1761723566878, updateTime=1761723566878, creator=18614031015, updator=18614031015), WebsiteProps(id=1190318170507411958, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1190317834937926113, code=minRunFlag, value=0, createTime=1761723566891, updateTime=1761723566891, creator=18614031015, updator=18614031015), WebsiteProps(id=1190318170473857520, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1190317834937926113, code=picServerUrl, value=https://castjournals.cast.org.cn/joweb/zgyxzz/EN/file/pic, createTime=1761723566883, updateTime=1761723566883, creator=18614031015, updator=18614031015), WebsiteProps(id=1190318170503217653, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1190317834937926113, code=silenceFlag, value=0, createTime=1761723566890, updateTime=1761723566890, creator=18614031015, updator=18614031015), WebsiteProps(id=1190318170465468911, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1190317834937926113, code=staticResourcePath, value=https://castjournals.cast.org.cn/joweb/cast_kjdb_en_623/, createTime=1761723566881, updateTime=1761723566881, creator=18614031015, updator=18614031015), WebsiteProps(id=1190318170482246130, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1190317834937926113, code=themeColor, value=null, createTime=1761723566885, updateTime=1761723566885, creator=18614031015, updator=18614031015), WebsiteProps(id=1190318170486440435, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1190317834937926113, code=themeStyle, value=null, createTime=1761723566886, updateTime=1761723566886, creator=18614031015, updator=18614031015)])], journalTitle=中国药学杂志, weixinUrl=null, journalUrl=http://www.zgyxzz.com.cn/, iacademicId=null, status=1, seqNo=null, journalTitleEn=Chinese Pharmaceutical Journal, journalPhotoCn=hRN1R6HnoNwYkve/JRn0DA==, journalPhotoEn=xSRntM4yOh2wVIE2w+OjYg==, journalFirstLetter=C, journalRecommend=null, journalNew=null, journalCollection=null, jcrJf=null, cjcrJf=null, jcrJfStr=null, cjcrJfStr=null, submissionFirstDecision=null, sciSubjectClassification=null, casSubjectClassification=null, citeScore=null, totalCitationFrequency=null, icpCode=null, psCode=null, advertisingLicenseCode=null, copyrightInformation=null, country=null, option=, provinceCode=null, provinceName=null, collectFlag=false), detailUrlCn=https://castjournals.cast.org.cn/joweb/zgyxzz/CN/10.11669/cpj.2024.11.011, detailUrlEn=https://castjournals.cast.org.cn/joweb/zgyxzz/EN/10.11669/cpj.2024.11.011, pdfUrlCn=https://castjournals.cast.org.cn/joweb/zgyxzz/CN/PDF/10.11669/cpj.2024.11.011, pdfUrlEn=https://castjournals.cast.org.cn/joweb/zgyxzz/EN/PDF/10.11669/cpj.2024.11.011, aliStartDate=null, aliEndDate=null, collectionFlag=false, citedCount=null, citedUrl=null, reference=null)
收藏切换
宏基因组测序法在药品污染细菌鉴定与溯源中的应用研究
收藏切换
PDF下载
严卓彦 1 , 谭宇翔 2 , 林吉恒 1 , 夏瑛瑛 1 , 孙瑛 1 , 崔洁 1 , 黄朱梁 1
中国药学杂志 | 论著 2024,59(11): 1041-1046
收起
收藏切换
中国药学杂志 | 论著 2024, 59(11): 1041-1046
宏基因组测序法在药品污染细菌鉴定与溯源中的应用研究
全屏
严卓彦1, 谭宇翔2, 林吉恒1, 夏瑛瑛1, 孙瑛1, 崔洁1, 黄朱梁1
作者信息
  • 1 舟山市食品药品检验检测研究院,国家海洋食品质量检验检测中心, 浙江 舟山 316012
  • 2 中国科学院深圳先进技术研究院,深圳合成生物学创新研究院,中国科学院定量工程生物学重点实验室, 广东 深圳 518055

    • 严卓彦,女,硕士 研究方向:药品微生物检测研究 Tel:(0580)2187150

Evaluation of the Application Value of Metagenomic Sequencing in Identification and Tracing of Bacteria Contaminating Drugs
Zhuoyan YAN1, Yuxiang TAN2, Jiheng LIN1, Yingying XIA1, Ying SUN1, Jie CUI1, Zhuliang HUANG1
Affiliations
  • 1 National Ocean Food Quality Inspection Center, Zhoushan Institute for Food and Drug Control, Zhoushan 316012, China
  • 2 CAS Key Laboratory of Quantitative Engineering Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China
出版时间: 2024-06-08 doi: 10.11669/cpj.2024.11.011
文章导航
收藏切换
摘要
收起

目的 评价宏基因组测序法在药品污染细菌鉴定和溯源中的应用。方法 利用宏基因组测序法和分离培养法同时对两批中成药及检测实验室环境取样中的细菌进行鉴定和分型。宏基因组测序结果通过MetaPhlan和StrainSifter软件进行菌株水平的分析。结果 经宏基因测序发现,药品与环境中均有大量的蜡样芽孢杆菌污染,且药品B18与环境样本HJ2中含有相同的蜡样芽孢杆菌菌组。培养法从药品和环境中共分离得到的5株蜡样芽孢杆菌,通过蜡样芽孢杆菌特异性聚合酶链式反应(PCR)扩增基因gyrA和panC序列构建NJ进化树,以及多位点序列分型(MLST),结果均显示药品B18中的蜡样芽孢杆菌菌株与环境样本HJ2的菌株同组。结论 宏基因组测序技术是可靠的,且相比培养法,能够更全面发现具有潜在风险的细菌物种,同时也能准确地进行菌株水平的溯源分析,在药品污染菌鉴定与溯源中具有重要的应用价值。

宏基因组测序  /  蜡样芽孢杆菌特异性PCR  /  多位点序列分型  /  药品污染  /  细菌鉴定  /  细菌溯源

OBJECTIVE To investigate metagenomic sequencing in the identification and tracing of bacteria contaminating drugs, and evaluate its application value. METHODS Both metagenomic sequencing and isolation culturing were employed to identify and type bacteria in two batches of Chinese patent medicines and environmental samples from the testing laboratory. The metagenomic sequencing results were analyzed at the strain level using MetaPhlan and StrainSifter software. RESULTS The analysis revealed a substantial contamination of Bacillus cereus-like organisms in both medicines and environmental samples, with the presence of the same B. cereus-like strain in medicine B18 and environmental sample HJ2. Through isolation culturing, five B. cereus-like strains were obtained from the medicines and environment. A phylogenetic tree was constructed based on the gyrA and panC gene sequences amplified by B. cereus-specific PCR, and multilocus sequence typing (MLST) results, all of which demonstrated that the B. cereus-like strain in medicine B18 belonged to the same group as one strain from the environment. CONCLUSION These findings indicate that metagenomic sequencing technology is reliable and has advantage over isolation culturing in comprehensively detecting potentially hazardous bacterial species and accurately performing strain-level traceability analysis. It holds significant application value in identification and tracing of pharmaceutical contamination.

Metagenomic sequencing  /  Bacillus cereus specific PCR  /  MLST  /  drug contamination  /  bacteria identification  /  bacteria tracing
严卓彦, 谭宇翔, 林吉恒, 夏瑛瑛, 孙瑛, 崔洁, 黄朱梁. 宏基因组测序法在药品污染细菌鉴定与溯源中的应用研究. 中国药学杂志, 2024 , 59 (11) : 1041 -1046 . DOI: 10.11669/cpj.2024.11.011
Zhuoyan YAN, Yuxiang TAN, Jiheng LIN, Yingying XIA, Ying SUN, Jie CUI, Zhuliang HUANG. Evaluation of the Application Value of Metagenomic Sequencing in Identification and Tracing of Bacteria Contaminating Drugs[J]. Chinese Pharmaceutical Journal, 2024 , 59 (11) : 1041 -1046 . DOI: 10.11669/cpj.2024.11.011
正文
收起
药品微生物控制是药品质量保证的重要组成部分,药品污染微生物不仅直接影响药品的有效性,更有可能危及用药患者的生命安全。虽然有 GMP 标准和药品召回制度对药品安全进行监管,药品微生物污染事件仍然时有发生。2013—2019 年统计的中国、美国、澳大利亚、日本和新加坡药品召回事件中,428次召回涉及微生物污染,占药品召回总数的 3.86%[1]。传统的微生物限度检查法只能对可以培养的微生物进行检测,而生态环境中可培养的微生物仅占所有微生物种类的约1%[2],因此非培养依赖技术如宏基因组技术在分析微生物多样性及其群落结构的研究中得到了广泛应用。
目前宏基因组研究主要集中在细菌的属、种水平,具体包括研究样本中细菌群落组成、物种丰度以及新物种的发现[3-4]、基因功能多样性[5]、细菌与宿主之间的相互作用[6]等。随着测序技术和分析软件的发展,将宏基因组测序技术应用在细菌的株水平已成为可能[7]。对样本和环境中相同菌种不同菌株的分析,有助于识别菌株间的亲缘关系及可能的污染途径。宏基因组菌株水平分析已经应用到食品污染[8]和院内感染爆发[9]的溯源分析中,但是尚未有药品中污染菌溯源分析的相关研究报道。
宏基因组菌株水平分析,主要可分为无参考基因组 (无参) 的组装分析和有参考基因组 (有参) 的比对分析两大类。有参比对的菌株分析方法又分为基于单核苷酸多态性(SNP)进行识别和基于基因组成 (genetic constitution) 差异进行识别两种分析方法。基于SNP的分析方法是菌株分析算法的主流,利用的是宏基因组数据中单碱基水平的信息,通过使用宏基因组片段序列和参考序列 (全基因组或标记基因) 的比对结果进行SNP位点的等位基因相对比例分析,最后根据这些比例信息,推算菌株的组成[10]。其中MetaPhlAn[11]可以标记基因定位到亚种级别。如果样品量足够,可通过StrainPhlAn[12]进一步实现菌株级别溯源。而以StrainSifter [13]为代表的基于全基因组信息进行深度的宏基因组菌株分析,虽然它1次只能针对1个参考基因组,但它能更全面利用测序数据,主要用于快速并准确地检测样品中是否存在特定菌株及其旁系。利用基因谱组成的判定方法有StrainPanDa[14]和PanPhlAn[15],其中PanPhlAn只能反映每个样品的基因谱以用于判定样品间的相似程度,无法定量和识别共存菌株,而StrainPanDa在其基础上进行了谱系解析,能反馈具体菌株的构成比例和对应基因谱。但这两个方法对具有同类菌株的样品数量要求较高。
本研究对两批中成药样本及两次环境取样进行分析,基于宏基因组测序数据,通过MetaPhlAn比较了样本和环境的相似菌种,并再利用StrainSifter确定潜在污染。然后通过传统培养法作为参照进一步确定中成药及环境中的污染细菌高度同源。为药品中污染细菌的鉴定和分型提供新的方法和技术参考。
1 实验材料、主要仪器与试剂
收起
1.1 实验材料
实验选取的两批中成药,分别为乳癖消片(编号B18)和胚宝胶囊(编号B20)。环境样本两次取样(分别编号HJ1和HJ2)均采集自本院药品微生物限度检查室,包括洁净工作台面、水浴锅、匀浆器、操作人员的无菌手套和无菌服、实验用胰酪胨大豆液体培养基(TSB)和pH 7.0氯化钠蛋白胨缓冲液。
1.2 仪器和试剂
水浴锅(DK-S24,上海精宏实验设备有限公司);匀浆器(HTY-761,浙江泰林生命科学有限公司);恒温培养箱(KB240)(德国BRAND公司);PCR扩增仪(My Cycler)、水平电泳仪(Mini-PROTEAN Tetra)、凝胶成像系统(Gel Doc XR)(美国BIO-RAD公司);全自动快速微生物质谱系统(VITEK MS)、全自动微生物鉴定及药敏分析系统(VITEK 2 Compact)、测序仪(NovaSeq 6000 sequencers)(美国Illumina公司);Ezup柱式细菌基因组DNA抽提试剂盒(B518255-0100)(上海生工);胰酪胨大豆液体培养基(TSB,批号 20210608)、胰酪胨大豆琼脂培养基(TSA,批号 20220505)、pH 7.0氯化钠蛋白胨缓冲液(批号 20230807)(青岛海博生物科技股份有限公司);营养琼脂(批号 230908,北京陆桥技术股份有限公司)。
2 实验方法
收起
2.1 样本采集
参照2020年版《中国药典》四部1105非无菌产品微生物限度检查法,在洁净区域无菌条件下称取样品10 g于封口无菌袋中,加入TSB制成100 mL供试液,然后按照以下方法混匀样品:胶囊类样品在45 ℃水浴锅中进行溶解,片剂类样品置于匀浆器中9 000 r·min-1混匀1 min。取1 mL供试液用pH 7.0氯化钠蛋白胨缓冲液进行10倍稀释,取原液和稀释液1 mL分别加入到无菌培养皿中,倾注胰酪胨大豆琼脂培养基(TSA)在(32.5±0.5) ℃进行5 d需氧菌培养。剩下的供试液(编号B18和B20)在32.5 ℃培养箱中培养18~24 h,然后送往上海生工生物工程有限公司进行基因组抽提和宏基因组测序分析。同时用无菌棉签擦拭检测人员无菌手套、无菌服、水浴锅、匀浆器、TSB培养基、pH 7.0氯化钠蛋白胨缓冲液等检测环境样本到不同的TSA平板上进行传统培养,然后将所有采样过的无菌棉签放入同一袋TSB培养液(编号HJ1和HJ2),在32.5 ℃培养18~24 h后送往上海生工进行宏基因组测序。
2.2 宏基因组测序及菌株水平分析
宏基因组测序法采用二代高通量测序法(NovaSeq 6000,Illumina),具体步骤包括基因组DNA片段化、文库构建、文库质控和测序。测序数据分析应用MetaPhlan(MetaPhlan-4.0.2)和StrainSifter软件。MetaPhlan(使用默认参数)是利用快速比对工具bowtie2与参比基因集(marker)数据库(mpa_vOct22_CHOCOPhlAnSGB_202212)进行比对,对宏基因组测序数据进行每个样品菌群定性和定量分析。具体步骤为参比基因组的确定、reads与marker的比对和含量的计算。StrainSifter(https://github.com/bhattlab/strainsifter)使用其snakemake流程(default参数),用GCF 002220285作为参考基因组进行全基因组的SNP分析。
2.3 单菌落分离、纯化和鉴定
按2020年版《中国药典》四部非无菌产品微生物限度检查法,需氧菌用TSA培养基在32.5 ℃培养5 d。每天观察平板并挑取单菌落到营养琼脂上进行分离纯化。将纯化的细菌单菌落通过生物质谱(VITEK MS)和微生物自动鉴定系统(VITEK 2 Compact)进行鉴定,同时用上海生工的柱式细菌基因组抽提试剂盒提取基因组,通过16srRNA全长PCR扩增测序、蜡样芽孢杆菌(Bacillus cereus)特异性PCR反应进行分子鉴定。蜡样芽孢杆菌特异性PCR采用扩增基因和引物见表1。PCR扩增产物送上海生工生物工程有限公司进行双向测序,将双向测序拼接结果序列通过NCBI的BLAST比对算法得到相似度最高的菌种,并利用MEGA 11软件采用K2P算法和Bootstrap=1 000参数构建Neighbor-Joining进化树。
2.4 蜡样芽孢杆菌菌株分型
根据PubMLST Databases中蜡样芽孢杆菌多位点序列分型(MLST)方案(http://pubmlst.org/organisms/bacillus-cereus/primers)选择7个管家基因(表2)进行PCR扩增,将正反双向引物扩增产物的测序结果进行拼接并上传至PubMLST Database进行基因序列比对,得到相应的Aelle ID,将比对过的7 个等位基因序列号上传后得到相应的序列类型MLST profiles。对于有基因变异和多态性位点变异的序列,向数据库提交新序列分离株的信息并申请新的分离株号(ID)和序列类型(ST)。采用在线BURST(n-4)算法进行分离株聚类分析。
3 结果
收起
3.1 宏基因组测序菌株水平分析结果
经过测序数据质控、拼接及聚类分析得到物种信息见表3,芽孢杆菌属(Bacillus)蜡样芽孢杆菌种在两个药品样本和环境样本HJ2中都是含量最高的属种,也是唯一在样品和检测环境中都出现的菌种。不动杆菌属(Acinetobacter)在检测环境样本HJ1和HJ2中的含量分别占第一和第二,但是在样品中没有出现,排除了其污染的可能性。MetaPhlan结果(表3)显示B18和HJ2中含有相同的蜡样芽孢杆菌组SGB7697,可推测存在检测环境对样品的污染。而使用信息更全面的StrainSifter方法验证结果(表4),显示B18与HJ2的基因组SNP差异在3个样本的两两比较中最小,进一步佐证了MetaPhlan结果的推测。综上所述,两种宏基因组分析方法的结果均证明B18与HJ2最接近,暗示了样品的污染源。
3.2 培养法菌株鉴定及分型
基于宏基因组测序结果,我们重点关注在样品和检测环境中存在的蜡样芽孢杆菌群。从样品B18、B20中各分离得到1株细菌(分别编号为B18和B20),从检测环境样本中分离得到3株细菌(分别编号为HJ-1、HJ-2、HJ-3)。以上5株菌的16srRNA测序、生物质谱与VITEK2 compact鉴定系统的结果均为蜡样芽孢杆菌群,无法鉴定到种。结合gyrA、panC和cry基因的PCR扩增结果,特异性PCR鉴定结果为蜡样芽孢杆菌(Bacillus cereus)或太平洋芽孢杆菌(Bacillus pacificus) (仅B20)(表5)。将5株菌的panC和gyrA基因序列(已上传至NCBI数据库并得到Genbank序列号)与NCBI下载的蜡样芽孢杆菌(Bacillus cereus)、苏云金芽孢杆菌(Bacillus thuringiensis)、蕈状芽孢杆菌(Bacilus mycoides)、太平洋芽孢杆菌(Bacillus pacificus)、魏德曼芽孢杆菌 (Bacillus wiedmanni)的panC和gyrA基因序列一起构建NJ进化树(见图1~2),结果均显示HJ-3与蜡样芽孢杆菌最接近,B20与太平洋芽孢杆菌最接近,B18与HJ-2最接近,其次为HJ-1。
将5株菌的7个管家基因序列在pubMLST网站上进行在线比对,结果显示3个菌株B18、HJ-2、HJ-3无对应的MLST profile,上传相关信息到pubMLST数据库得到新的ST型号和分离株号。allele结果显示7个管家基因中有3个B18与HJ-2序列相同,BURST聚类结果显示B18和HJ-2为同一组,其余皆为独立基因型的菌株(表6)。
4 讨论
收起
蜡样芽孢杆菌(Bacillus cereus)是一种兼性厌氧的杆状革兰阳性细菌,在土壤、水、植物、饲料以及各种食品中大量存在;该菌可以产生内生孢子,对环境具有很强的适应性,能够抵抗高温、紫外线、电磁辐射和有害化学物质等不利条件[16]。它与苏云金芽孢杆菌、蕈状芽孢杆菌、炭疽芽孢杆菌等同属蜡样芽孢杆菌群。蜡样芽孢杆菌群的16SrRNA基因相似性非常高,只能将他们鉴定为同一个物种,并不能很好地区分蜡样芽孢杆菌群[17],需要结合芽孢杆菌特异性PCR如gyrA基因[14]、gyrB基因[18]、panC基因[19]等信息进行区分。且蜡样芽孢杆菌群的蛋白肽谱也高度相似,生物质谱和VITEK 2 compact鉴定系统都无法将它们进行区分。蜡样芽孢杆菌与苏云金芽孢杆菌的基因组几乎完全相同,唯一的区别是苏云金芽孢杆菌在质粒上有编码蛋白毒素结晶体的cry基因[20]。因此,本研究对蜡样芽孢杆菌的鉴定基于gyrA、panC和cry基因的特异性PCR反应结果。基于gyrA和panC基因的NJ进化树显示药品B18中分离的菌株与环境中分离的菌株HJ-2最接近,其次是HJ-1,药品B20与另外4株菌距离较远,而与蜡样芽孢杆菌群的另一个种太平洋芽孢杆菌(Bacillus pacificus)比较接近。对5株蜡样芽孢杆菌菌株的分型溯源采用国际通用的MLST法,得到蜡样芽孢杆菌7个管家基因序列,采用在线BURST(n-4)算法进行聚类分析,得到B18与HJ-2属于同一组的结果。因此高度怀疑B18中的蜡样芽孢杆菌可能来源于检测环境中的污染。
宏基因组测序法对样品中的全部基因组进行测序、拼接和比对,因此可以检测到无法通过培养法分离得到的细菌,比如环境中含量较高的不动杆菌(Acinetobacter,相对丰度大于38%)及含量较低的假单胞菌(Pseudomonas,相对丰度3%)和尼尔菌(Niallia,相对丰度2%),以及药品B18中少量的巨大芽孢杆菌(Priestia megaterium,相对丰度1%)。对宏基因组测序结果进行菌株水平的分析可以预测样本之间可能的污染途径,比如MetaPhlan的分析结果显示B18和环境样本中含有相同的蜡样芽孢杆菌群组SGB7697,而B20以蜡样芽孢杆菌群组SGB7703为主,该结果与培养法的溯源结果一致,都显示样本B18中的蜡样芽孢杆菌可能来源于检测环境。B20还含有少量的蜡样芽孢杆菌群组SGB7697(相对丰度5%),但是丰度太低,未能通过培养法进行验证,也说明了宏基因组测序法能够得到比培养法更多的菌株信息。
药品B20中较高含量的蜡样芽孢杆菌与检测环境中的蜡样芽孢杆菌不属于同一型,可能是药品自身携带的,B18中还检出少量的巨大芽孢杆菌。芽孢杆菌会在人体基础病变引起机体防卫功能降低时导致各种机会感染[23]。所以,虽然上述污染菌株多为环境常见菌,没有危害性较大的致病菌,但也属于条件致病菌,其用药对象若是婴幼儿及衰弱患者,就会导致使用该药品的患者受到感染,属于药品中的不可接受微生物[24-25],因此应该引起生产厂家的高度重视。
基于株水平的宏基因组学方法不仅能准确识别菌株间亲缘关系,还能节省样本分离纯培养时间,同时有助于更全面发现具有潜在风险的细菌物种,在药品微生物鉴定与溯源分析中具有重要的应用价值。
基金
收起
  • 浙江省药监局科技计划项目资助(2023010)
文献
收起
参考文献 引证文献
排序方式：
[1]
WU Y W, WANG Y, CHE Y, et al. Review and Analysis of International Micobial Contamination-related Drug recalls from 2013 to 2019[J]. Chin J Mod Appl Pharm(中国现代应用药学), 2021, 38: 1870-1877.
[2]
MARCY Y, OUVERNEY C, BIK E M, et al. Dissecting biological “dark matter” with single-cell genetic analysis of rare and uncultivated TM7 microbes from the human mouth[J]. Proc Natl Acad Sci USA, 2007, 104(29): 11889-11894.
[3]
NAYFACH S, SHI Z J, SESHADRI R, et al. New insights from uncultivated genomes of the global human gut microbiome[J]. Nature, 2019, 568(7753): 505-510.
[4]
CYCIL L M, DASSARMA S, PECHER W, et al. Metagenomic insights into the diversity of halophilic microorganisms indigenous to the Karak salt mine, Pakistan[J]. Front Microbiol, 2020, 11: 1567.
[5]
LOMBARDINO J, BIJLANI S, SINGH N K, et al. Genomic characterization of potential plant growth-promoting features of Sphingomonas strains isolated from the international space station[J]. Microbiol Spectr, 2022, 10(1): e0199421. DOI: 10.1128/spectrum.01994-21.
[6]
KARLSSON FH, FAK F, NOOKAEW I, et al. Symptomatic atherosclerosis is associated with an altered gut metagenome[J]. Nat Commun, 2012, 3(1): 1245. DOI: 10.1038/ncomms2266.
[7]
QIAN X W, WU Y R, HAO T Y, et al. Strain-level bacterial analysis from shotgun metagenomics: a review[J]. Dis Surveill(疾病监测), 2022, 37: 1408-1412.
[8]
BUYTAERS F E, SALTYKOVA A, MATTHEUS W, et al. Application of a strain-level shotgun metagenomics approach on food samples: resolution of the source of a Salmonella food-borne outbreak[J]. Microb Genom, 2021, 7(4): 000547. DOI: 10.1099/mgen.0.000547.
[9]
MU A, KWONG J C, ISLES N S, et al. Reconstruction of the genomes of drug-resistant pathogens for outbreak investigation through metagenomic sequencing[J]. mSphere, 2019, 4(1): e00529-18. DOI: 10.1128/mSphere.00529-18.
[10]
TAN Y X, HU H, LI C H, et al. Research progress and applications of strain analysis based on metagenomic data[J]. Chin J Biotech(生物工程学报), 2020, 36(12): 2610-2621.
[11]
TRUONG D T, FRANZOSA E A, TICKLE T L, et al. MetaPhlAn2 for enhanced metagenomic taxonomic profiling[J]. Nat Methods, 2015, 12(10): 902-903.
[12]
TRUONG D T, TETT A, PASOLLI E, et al. Microbial strain-level population structure and genetic diversity from metagenomes[J]. Genome Res, 2017, 27(4): 626-638.
[13]
TAMBURINI F B, ANDERMANN T M, TKACHENKO E, et al. Precision identification of diverse bloodstream pathogens in the gut microbiome[J]. Nat Med, 2018, 24(12): 1809-1814.
[14]
HU H, TAN Y X, LI C H, et al. StrainPanDA: linked reconstruction of strain composition and gene content profiles via pangenome-based decomposition of metagenomic data[J]. iMeta, 2022. https://doi.org/10.1002/imt2.41.
[15]
SCHOLZ M, WARD D V, PASOLLI E, et al. Strain-level microbial epidemiology and population genomics from shotgun metagenomics[J]. Nat Methods, 2016, 13(5): 435-438.
[16]
YAN J T, QIAO K, CAI Y F. Application of rpoB, gyrA and cheA genes in identifying Bacillus genus[J]. Acta Agric Zhejiangensis(浙江农业学报), 2022, 34(1): 128-140
[17]
KUO W S, CHAK K F. Identification of novel cry-type genes from Bacillus thuringiensis stains on the basis of restriction fragment length polymorphism of the PCR-amplified DNA[J]. Appl Environ Microbiol, 1996, 62(4): 1369-1377.
[18]
ZHANG L Y, SONG L L, JIA W J, et al. Research process on detection methods of Bacillus cereus[J]. Microbiol China(微生物学通报), 2021, 48(4): 1360-1372.
[19]
HUANG J J, LUO J, HE H X. Research progress on detection methods for Bacillus cereus[J]. China Anim Husb Vet Med(中国畜牧兽医), 2018, 45(3): 635-642.
[20]
LU J Q, LI S C, WANG D H, et al. Combined 16S rDNA and gyrB gene sequence to identify a strain of Bacillus cereus[J]. J Henan Univ Sci Technol Nat Sci(河南科技大学学报自然科学版), 2018, 39(1): 74-83.
[21]
VERA A, PABLO S F, MARIA R C, et al. Development of a high-resolution melting-based approach for efficient differentiation among Bacillus cereus group isolates[J]. Foodborne Pathog Dis, 2012, 9(9): 777-785.
[22]
CHEN M L, TSEN H Y. Discrimination of Bacillus cereus and Bacillus thuringiensis with 16S rRNA and gyrB gene based PCR primers and sequencing of their annealing sites[J]. J Appl Microbiol, 2002, 92: 912-919.
[23]
MEI L. Condition the etiology of diarrhea caused by pathogenic bacteria[J]. Clin Med(临床医学), 2004, 24(10): 49.
[24]
SUTLON S. What is an “objectionable organism”?[J]. Am Pharm Rev, 2012, 15(6): 1-13.
[25]
ROESTI D, GOVERDE M. Pharmaceutical Microbiological Quality Assurance and Control[M]. New Jersey: John Wiley & Sons, Inc., 2019: 371-400.
2024年第59卷第11期
PDF下载
132
63
引用本文
BibTeX
文章信息
doi: 10.11669/cpj.2024.11.011
  • 接收时间：2023-12-11
  • 首发时间：2025-11-25
  • 出版时间：2024-06-08
补充材料
相关文章
文章信息
作者
出版历史
  • 收稿日期：2023-12-11
基金
浙江省药监局科技计划项目资助(2023010)
作者信息
    1 舟山市食品药品检验检测研究院,国家海洋食品质量检验检测中心, 浙江 舟山 316012
    2 中国科学院深圳先进技术研究院,深圳合成生物学创新研究院,中国科学院定量工程生物学重点实验室, 广东 深圳 518055
参考文献
分享链接
https://castjournals.cast.org.cn/joweb/zgyxzz/CN/10.11669/cpj.2024.11.011
分享至
全文二维码

扫描看全文

引用本文
BibTeX
本文的引用情况
2种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
关闭全屏
相关链接
论文
最新文章
热读文章
热点专题
内参
蓝皮书
产业发展报告
传播力报告
期刊分级目录
资讯
学术新闻
行业新闻
学术会议
行业会议
关于
关于我们
联系我们
北京市海淀区学院南路86号
100081
010-62199257
qkjq@cast.org.cn

中国科学技术协会版权所有 京ICP 备10216604号-4 海淀分局备案 1101084647
科技导报社主办 中国科协信息中心技术支持