首页 期刊 论文 学科刊群 资讯 加盟 关于
EN
image
Article(id=1200147771740222434, tenantId=1146029695717560320, journalId=1190317699101192196, issueId=1200147768326062257, articleNumber=1001-2494(2024)09-0827-07, orderNo=null, doi=10.11669/cpj.2024.09.008, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1699200000000, receivedDateStr=2023-11-06, revisedDate=null, revisedDateStr=null, acceptedDate=null, acceptedDateStr=null, onlineDate=1764067126449, onlineDateStr=2025-11-25, pubDate=1715097600000, pubDateStr=2024-05-08, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1764067126449, onlineIssueDateStr=2025-11-25, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1764067126449, creator=13701087609, updateTime=1764067126449, updator=13701087609, issue=Issue{id=1200147768326062257, tenantId=1146029695717560320, journalId=1190317699101192196, year='2024', volume='59', issue='9', pageStart='757', pageEnd='856', issueExtLink='null', onlineDate='null', pubDate='null', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1764067125636, creator=13701087609, updateTime=1764067301065, updator=13701087609, preIssue=null, nextIssue=null, ext={EN=IssueExt(id=1200148504178950495, tenantId=1146029695717560320, journalId=1190317699101192196, issueId=1200147768326062257, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1200148504178950496, tenantId=1146029695717560320, journalId=1190317699101192196, issueId=1200147768326062257, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null}, startPage=827, endPage=833, ext={EN=ArticleExt(id=1200147772038018032, articleId=1200147771740222434, tenantId=1146029695717560320, journalId=1190317699101192196, language=EN, title=Fingerprint and Phlorizin Content Assay of Tujia Ethnomedicinal Herb Impatientis Pritzelii Rhizoma, columnId=null, journalTitle=Chinese Pharmaceutical Journal, columnName=null, runingTitle=null, highlight=null, articleAbstract=

OBJECTIVE To establish the fingerprint and content determination method of Impatientis Pritzelii Rhizoma to improve the quality control method of Tujia ethnomedicinal herb Impatientis Pritzelii Rhizoma. METHODS The fingerprint was established by UPLC with ACQUITY UPLC BEH C18 column. Phlorizin content assay was performed by HPLC with Eclipse XDB-C18 column. The mobile phases for both methods were composed of acetonitrile and 0.1% formic acid with different gradient elution procedures. The detection wavelength was set at 283 nm. The comparative study was carried out between Impatientis Pritzelii Rhizoma and its adulterants (such as the roots and rhizomes of Impatiens pinfanensis, I. uliginosa, I. siculifer and I. noli-tangere). RESULTS Nine main chromatographic peaks in the fingerprint could be served as the characteristic peaks of Impatientis Pritzelii Rhizoma, four of which were corresponding to catechin, epicatechin, scopolamine and phlorizin, respectively. The established fingerprint could reflect the distribution of the chemical components of Impatientis Pritzelii Rhizoma, which was distinguished from four adulterants. Phlorizin was found and determined in Impatientis Pritzelii Rhizoma for the first time. The contents in 16 batches of Impatientis Pritzelii Rhizoma ranged from 0.007% to 0.085%, while no phloridin was found in the adulterants. CONCLUSION The established methods for the fingerprint and phlorizin content determination of Impatientis Pritzelii Rhizoma are specific, accurate and effective, which could be used for its quality control.

, correspAuthors=Min HU, Jun TANG, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, authorCompany=null, fund=null, authors=null, authorsList=Ling XIAO, Menghan LIU, Menglin XU, Min HU, Jun TANG), CN=ArticleExt(id=1200147773728321601, articleId=1200147771740222434, tenantId=1146029695717560320, journalId=1190317699101192196, language=CN, title=土家族药材冷水七指纹图谱及根皮苷含量测定的研究, columnId=1190352405612040510, journalTitle=中国药学杂志, columnName=论著, runingTitle=null, highlight=null, articleAbstract=

目的 提高土家族药材冷水七的质量控制方法,建立该药材指纹图谱及含量测定方法。方法 指纹图谱采用超高效液相色谱法(UPLC),ACQUITY UPLC BEH C18柱,根皮苷含量测定采用高效液相色谱法(HPLC),Eclipse XDB-C18柱,流动相均为乙腈-0.1%甲酸梯度洗脱,检测波长为283 nm,对冷水七及其易混品(同属块节凤仙花、滇水金凤、黄金凤、水金凤的根茎)进行比较研究。结果 指纹图谱中9个特征峰可作为冷水七的特征峰,其中4个峰分别与儿茶素、表儿茶素、东莨菪素及根皮苷相对应;该指纹图谱反映了冷水七化学成分的分布,且可与其易混品区分。根皮苷为首次在冷水七中发现,16批冷水七中根皮苷含量范围为0.007%~0.085%,易混品未检出根皮苷。结论 本研究建立的冷水七指纹图谱及其根皮苷含量测定方法专属、准确、有效,可用于冷水七药材的质量控制。

, correspAuthors=胡敏, 汤俊, authorNote=null, correspAuthorsNote=
*汤俊,男,博士,副教授 研究方向:中药、天然药物化学成分及活性研究;
胡敏,女,硕士,主任药师 研究方向:药品质量分析 Tel:(027)87705232
, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=mRYIzjooSjSEBLmaWCwkOA==, magXml=oZGZhmLQsQFjdrA8NSLr6Q==, pdfUrl=null, pdf=plQwCSzHW+0TXrAIO4HK5g==, pdfFileSize=2081521, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=E/F5VsTaywilaZPU+/0y7Q==, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=SttxywMa69Zh+/uiTziZpQ==, mapNumber=null, authorCompany=null, fund=null, authors=

肖凌,女,副主任药师 研究方向:中药质量评价新技术新方法的研究及应用

, authorsList=肖凌, 刘梦涵, 徐梦麟, 胡敏, 汤俊)}, authors=[Author(id=1200147774240026711, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147771740222434, orderNo=0, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1200147774365855837, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147771740222434, authorId=1200147774240026711, language=EN, stringName=Ling XIAO, firstName=Ling, middleName=null, lastName=XIAO, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, address=1 NMPA Key Laboratory of Quality Control of Chinese Medicine Hubei, Engineering Research Center for Drug Quality Control, Hubei Institute for Drug Control, Wuhan 430075, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1200147774537822308, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147771740222434, authorId=1200147774240026711, language=CN, stringName=肖凌, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, address=1 湖北省药品监督检验研究院, 国家药品监督管理局中药质量控制重点实验室, 湖北省药品质量检测与控制工程技术研究中心, 武汉 430075, bio={"content":"

肖凌,女,副主任药师 研究方向:中药质量评价新技术新方法的研究及应用

"}, bioImg=null, bioContent=

肖凌,女,副主任药师 研究方向:中药质量评价新技术新方法的研究及应用

, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1200147773996757063, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147771740222434, xref=1, ext=[AuthorCompanyExt(id=1200147774005145673, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147771740222434, companyId=1200147773996757063, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1 NMPA Key Laboratory of Quality Control of Chinese Medicine Hubei, Engineering Research Center for Drug Quality Control, Hubei Institute for Drug Control, Wuhan 430075, China), AuthorCompanyExt(id=1200147774013534283, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147771740222434, companyId=1200147773996757063, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1 湖北省药品监督检验研究院, 国家药品监督管理局中药质量控制重点实验室, 湖北省药品质量检测与控制工程技术研究中心, 武汉 430075)])]), Author(id=1200147774764314734, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147771740222434, orderNo=1, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1200147774860783729, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147771740222434, authorId=1200147774764314734, language=EN, stringName=Menghan LIU, firstName=Menghan, middleName=null, lastName=LIU, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=2, address=2 Key Laboratory of Combinatorial Biosynthesis and Drug Discovery (Wuhan University), Ministry of Education, School of Pharmaceutical Sciences, Wuhan University, Wuhan 430071, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1200147775074693237, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147771740222434, authorId=1200147774764314734, language=CN, stringName=刘梦涵, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=2, address=2 武汉大学药学院,组合生物合成与新药发现教育部重点实验室, 武汉 430071, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1200147774126780496, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147771740222434, xref=2, ext=[AuthorCompanyExt(id=1200147774135169105, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147771740222434, companyId=1200147774126780496, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2 Key Laboratory of Combinatorial Biosynthesis and Drug Discovery (Wuhan University), Ministry of Education, School of Pharmaceutical Sciences, Wuhan University, Wuhan 430071, China), AuthorCompanyExt(id=1200147774143557715, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147771740222434, companyId=1200147774126780496, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2 武汉大学药学院,组合生物合成与新药发现教育部重点实验室, 武汉 430071)])]), Author(id=1200147775183745145, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147771740222434, orderNo=2, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1200147775301185660, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147771740222434, authorId=1200147775183745145, language=EN, stringName=Menglin XU, firstName=Menglin, middleName=null, lastName=XU, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=2, address=2 Key Laboratory of Combinatorial Biosynthesis and Drug Discovery (Wuhan University), Ministry of Education, School of Pharmaceutical Sciences, Wuhan University, Wuhan 430071, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1200147775401848959, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147771740222434, authorId=1200147775183745145, language=CN, stringName=徐梦麟, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=2, address=2 武汉大学药学院,组合生物合成与新药发现教育部重点实验室, 武汉 430071, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1200147774126780496, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147771740222434, xref=2, ext=[AuthorCompanyExt(id=1200147774135169105, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147771740222434, companyId=1200147774126780496, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2 Key Laboratory of Combinatorial Biosynthesis and Drug Discovery (Wuhan University), Ministry of Education, School of Pharmaceutical Sciences, Wuhan University, Wuhan 430071, China), AuthorCompanyExt(id=1200147774143557715, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147771740222434, companyId=1200147774126780496, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2 武汉大学药学院,组合生物合成与新药发现教育部重点实验室, 武汉 430071)])]), Author(id=1200147775494123651, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147771740222434, orderNo=3, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=1, authorType=1, ext={EN=AuthorExt(id=1200147775586398344, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147771740222434, authorId=1200147775494123651, language=EN, stringName=Min HU, firstName=Min, middleName=null, lastName=HU, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, *, address=1 NMPA Key Laboratory of Quality Control of Chinese Medicine Hubei, Engineering Research Center for Drug Quality Control, Hubei Institute for Drug Control, Wuhan 430075, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1200147775674478731, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147771740222434, authorId=1200147775494123651, language=CN, stringName=胡敏, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, *, address=1 湖北省药品监督检验研究院, 国家药品监督管理局中药质量控制重点实验室, 湖北省药品质量检测与控制工程技术研究中心, 武汉 430075, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1200147773996757063, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147771740222434, xref=1, ext=[AuthorCompanyExt(id=1200147774005145673, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147771740222434, companyId=1200147773996757063, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1 NMPA Key Laboratory of Quality Control of Chinese Medicine Hubei, Engineering Research Center for Drug Quality Control, Hubei Institute for Drug Control, Wuhan 430075, China), AuthorCompanyExt(id=1200147774013534283, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147771740222434, companyId=1200147773996757063, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1 湖北省药品监督检验研究院, 国家药品监督管理局中药质量控制重点实验室, 湖北省药品质量检测与控制工程技术研究中心, 武汉 430075)])]), Author(id=1200147775817085072, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147771740222434, orderNo=4, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=1, authorType=1, ext={EN=AuthorExt(id=1200147775942914196, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147771740222434, authorId=1200147775817085072, language=EN, stringName=Jun TANG, firstName=Jun, middleName=null, lastName=TANG, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=2, *, address=2 Key Laboratory of Combinatorial Biosynthesis and Drug Discovery (Wuhan University), Ministry of Education, School of Pharmaceutical Sciences, Wuhan University, Wuhan 430071, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1200147776014217367, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147771740222434, authorId=1200147775817085072, language=CN, stringName=汤俊, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=2, *, address=2 武汉大学药学院,组合生物合成与新药发现教育部重点实验室, 武汉 430071, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1200147774126780496, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147771740222434, xref=2, ext=[AuthorCompanyExt(id=1200147774135169105, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147771740222434, companyId=1200147774126780496, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2 Key Laboratory of Combinatorial Biosynthesis and Drug Discovery (Wuhan University), Ministry of Education, School of Pharmaceutical Sciences, Wuhan University, Wuhan 430071, China), AuthorCompanyExt(id=1200147774143557715, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147771740222434, companyId=1200147774126780496, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2 武汉大学药学院,组合生物合成与新药发现教育部重点实验室, 武汉 430071)])])], keywords=[Keyword(id=1200147776119074972, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147771740222434, language=EN, orderNo=1, keyword=Impatientis Pritzelii Rhizoma), Keyword(id=1200147776181989537, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147771740222434, language=EN, orderNo=2, keyword=Impatiens), Keyword(id=1200147776249098404, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147771740222434, language=EN, orderNo=3, keyword=phlorizin), Keyword(id=1200147776332984487, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147771740222434, language=EN, orderNo=4, keyword=fingerprint), Keyword(id=1200147776425259182, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147771740222434, language=CN, orderNo=1, keyword=冷水七), Keyword(id=1200147776559476914, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147771740222434, language=CN, orderNo=2, keyword=凤仙花属), Keyword(id=1200147776664334518, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147771740222434, language=CN, orderNo=3, keyword=根皮苷), Keyword(id=1200147776760803513, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147771740222434, language=CN, orderNo=4, keyword=指纹图谱)], refs=[Reference(id=1200147778925064438, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147771740222434, doi=null, pmid=null, pmcid=null, year=2017, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[1], rfOrder=0, authorNames=ZHANG J C, journalName=Study on anti-rheumatoid active ingredients of Lengshuiqi and anti-tumor components of Dianthi Herba, refType=null, unstructuredReference=ZHANG J C. Study on anti-rheumatoid active ingredients of Lengshuiqi and anti-tumor components of Dianthi Herba[D]. Wuhan: Hubei University of Chinese Medicine, 2017., articleTitle=null, refAbstract=null), Reference(id=1200147779013144826, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147771740222434, doi=null, pmid=null, pmcid=null, year=2012, volume=23, issue=5, pageStart=1272, pageEnd=1273, url=null, language=null, rfNumber=[2], rfOrder=1, authorNames=LEI G L, WEI M Y, GUO H J, journalName=Lishizhen Med Mater Med Res(时珍国医国药), refType=null, unstructuredReference=LEI G L, WEI M Y, GUO H J, et al. Research and utilization of ethnomedicine“Lengshuiqi”[J]. Lishizhen Med Mater Med Res(时珍国医国药), 2012, 23(5):1272-1273., articleTitle=Research and utilization of ethnomedicine“Lengshuiqi”, refAbstract=null), Reference(id=1200147779092836602, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147771740222434, doi=null, pmid=null, pmcid=null, year=2016, volume=41, issue=1, pageStart=83, pageEnd=86, url=null, language=null, rfNumber=[3], rfOrder=2, authorNames=ZHANG J C, HE W J, LIU Y W, journalName=China J Chin Mater Med(中国中药杂志), refType=null, unstructuredReference=ZHANG J C, HE W J, LIU Y W, et al. Chemical constituents from Impatiens pritzllii var. hupehensis[J]. China J Chin Mater Med(中国中药杂志), 2016, 41(1):83-86., articleTitle=Chemical constituents from Impatiens pritzllii var. hupehensis, refAbstract=null), Reference(id=1200147779155751165, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147771740222434, doi=null, pmid=null, pmcid=null, year=2007, volume=9, issue=4, pageStart=379, pageEnd=385, url=null, language=null, rfNumber=[4], rfOrder=3, authorNames=ZHOU X F, ZHAO X Y, TANG L, journalName=J Asian Nat Prod Res, refType=null, unstructuredReference=ZHOU X F, ZHAO X Y, TANG L, et al. Three new triterpenoid saponins from the rhizomes of Impatiens pritzellii var. Hupehensis[J]. J Asian Nat Prod Res, 2007, 9(4):379-385., articleTitle=Three new triterpenoid saponins from the rhizomes of Impatiens pritzellii var. Hupehensis, refAbstract=null), Reference(id=1200147779235442944, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147771740222434, doi=null, pmid=null, pmcid=null, year=2009, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[5], rfOrder=4, authorNames=ZHOU X F, journalName=Material foundation study of Rhizoma Impatiens pritzellii and Radix Actinidia chinensis, refType=null, unstructuredReference=ZHOU X F. Material foundation study of Rhizoma Impatiens pritzellii and Radix Actinidia chinensis[D]. Wuhan: Huazhong University of Science and Technology, 2009., articleTitle=null, refAbstract=null), Reference(id=1200147779319329027, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147771740222434, doi=null, pmid=null, pmcid=null, year=2005, volume=30, issue=8, pageStart=584, pageEnd=586, url=null, language=null, rfNumber=[6], rfOrder=5, authorNames=ZHAOX Y, SUN H D, WU J Z, journalName=China J Chin Mater Med(中国中药杂志), refType=null, unstructuredReference=ZHAOX Y, SUN H D, WU J Z. Studies on chemical constituents from rhizome of Impatiens pritzellii var. hupehensis[J]. China J Chin Mater Med(中国中药杂志), 2005, 30(8):584-586., articleTitle=Studies on chemical constituents from rhizome of Impatiens pritzellii var. hupehensis, refAbstract=null), Reference(id=1200147779403215108, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147771740222434, doi=null, pmid=null, pmcid=null, year=2008, volume=14, issue=5, pageStart=62, pageEnd=64, url=null, language=null, rfNumber=[7], rfOrder=6, authorNames=WEN D J, journalName=J Med Pharm Chin Minor(中国民族医药杂志), refType=null, unstructuredReference=WEN D J. Research progress of Tujia medicine“Lengshuiqi”[J]. J Med Pharm Chin Minor(中国民族医药杂志), 2008, 14(5):62-64., articleTitle=Research progress of Tujia medicine“Lengshuiqi”, refAbstract=null), Reference(id=1200147779516461319, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147771740222434, doi=null, pmid=null, pmcid=null, year=2018, volume=null, issue=null, pageStart=112, pageEnd=null, url=null, language=null, rfNumber=[8], rfOrder=7, authorNames=null, journalName=Medical Products Administration of Hubei Province, Quality Standard of Chinese Crude Drugs in Hubei Province(湖北省中药材质量标准), refType=null, unstructuredReference=Medical Products Administration of Hubei Province, Quality Standard of Chinese Crude Drugs in Hubei Province(湖北省中药材质量标准)[M]. Beijing: China Medical Science and Technology Press, 2018:112., articleTitle=null, refAbstract=null), Reference(id=1200147779625513223, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147771740222434, doi=null, pmid=null, pmcid=null, year=2009, volume=28, issue=1, pageStart=51, pageEnd=54, url=null, language=null, rfNumber=[9], rfOrder=8, authorNames=XU R, WANG J, WAN D R, journalName=J South-Cent Univ Natl(Nat Sci Ed)(中南民族大学学报自然科学版), refType=null, unstructuredReference=XU R, WANG J, WAN D R. Study on quality standard of ethnomedicine“Lengshuiqi”[J]. J South-Cent Univ Natl(Nat Sci Ed)(中南民族大学学报自然科学版), 2009, 28(1):51-54., articleTitle=Study on quality standard of ethnomedicine“Lengshuiqi”, refAbstract=null), Reference(id=1200147779747148042, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147771740222434, doi=null, pmid=null, pmcid=null, year=2021, volume=147, issue=null, pageStart=109783, pageEnd=null, url=null, language=null, rfNumber=[10], rfOrder=9, authorNames=HERRERA-GONZÁLEZ A, NÚÑEZ-LÓPEZ G, NÚÑEZ-DALLOS N, journalName=Enzyme Microb Technol, refType=null, unstructuredReference=HERRERA-GONZÁLEZ A, NÚÑEZ-LÓPEZ G, NÚÑEZ-DALLOS N, et al. Enzymatic synthesis of phlorizin fructosides[J]. Enzyme Microb Technol, 2021, 147:109783. DOI: 10.1016/j.enzmictec.2021.109783., articleTitle=Enzymatic synthesis of phlorizin fructosides, refAbstract=null), Reference(id=1200147779910725900, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147771740222434, doi=null, pmid=null, pmcid=null, year=2023, volume=28, issue=3, pageStart=919, pageEnd=null, url=null, language=null, rfNumber=[11], rfOrder=10, authorNames=ONGAY K K, GRANATO D, BARRETO G E, journalName=Molecules, refType=null, unstructuredReference=ONGAY K K, GRANATO D, BARRETO G E. Comparison of antioxidant capacity and network pharmacology of phloretin and phlorizin against neuroinflammation in traumatic brain injury[J]. Molecules, 2023, 28(3):919. DOI: 10.3390/molecules28030919., articleTitle=Comparison of antioxidant capacity and network pharmacology of phloretin and phlorizin against neuroinflammation in traumatic brain injury, refAbstract=null), Reference(id=1200147779982029070, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147771740222434, doi=null, pmid=null, pmcid=null, year=2023, volume=11, issue=1, pageStart=143, pageEnd=null, url=null, language=null, rfNumber=[12], rfOrder=11, authorNames=HABTEMARIAM S, journalName=Biomedicines, refType=null, unstructuredReference=HABTEMARIAM S. The molecular pharmacology of phloretin: anti-inflammatory mechanisms of action[J]. Biomedicines, 2023, 11(1):143. DOI: 10.3390/biomedicines11010143., articleTitle=The molecular pharmacology of phloretin: anti-inflammatory mechanisms of action, refAbstract=null), Reference(id=1200147780074303761, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147771740222434, doi=null, pmid=null, pmcid=null, year=2023, volume=null, issue=null, pageStart=225, pageEnd=226, url=null, language=null, rfNumber=[13], rfOrder=12, authorNames=LIU M H, XIAO L, TANG J, journalName=CCS 14th National Symposium on Natural Organic Chemistry, refType=null, unstructuredReference=LIU M H, XIAO L, TANG J. Excavation of saponins components in Lengshuiqi by UPLC-Q-TOF-MS[C]// CCS 14th National Symposium on Natural Organic Chemistry. Wuhan: Chinese Chemical Society, 2023, 225-226., articleTitle=Excavation of saponins components in Lengshuiqi by UPLC-Q-TOF-MS, refAbstract=null)], funds=[Fund(id=1200147778740515057, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147771740222434, awardId=2020ACA007, language=CN, fundingSource=湖北省重点研发计划项目“湖北中药质控标准物质研制及技术平台建立”资助(2020ACA007), fundOrder=null, country=null), Fund(id=1200147778811818228, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147771740222434, awardId=NIFDC-TCM2021-049-MZY011, language=CN, fundingSource=国家药品监督管理局注册司民族药专项“特色民族药检验方法的示范性研究”资助(NIFDC-TCM2021-049-MZY011), fundOrder=null, country=null)], companyList=[AuthorCompany(id=1200147773996757063, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147771740222434, xref=1, ext=[AuthorCompanyExt(id=1200147774005145673, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147771740222434, companyId=1200147773996757063, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1 NMPA Key Laboratory of Quality Control of Chinese Medicine Hubei, Engineering Research Center for Drug Quality Control, Hubei Institute for Drug Control, Wuhan 430075, China), AuthorCompanyExt(id=1200147774013534283, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147771740222434, companyId=1200147773996757063, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1 湖北省药品监督检验研究院, 国家药品监督管理局中药质量控制重点实验室, 湖北省药品质量检测与控制工程技术研究中心, 武汉 430075)]), AuthorCompany(id=1200147774126780496, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147771740222434, xref=2, ext=[AuthorCompanyExt(id=1200147774135169105, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147771740222434, companyId=1200147774126780496, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2 Key Laboratory of Combinatorial Biosynthesis and Drug Discovery (Wuhan University), Ministry of Education, School of Pharmaceutical Sciences, Wuhan University, Wuhan 430071, China), AuthorCompanyExt(id=1200147774143557715, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147771740222434, companyId=1200147774126780496, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2 武汉大学药学院,组合生物合成与新药发现教育部重点实验室, 武汉 430071)])], figs=[ArticleFig(id=1200147776903409853, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147771740222434, language=EN, label=Fig.1, caption=UPLC chromatograms of Impatientis Pritzelii Rhizoma and mixed reference substance

2-Catechin; 3-Epicatechin; 4-Scopolamine; 7-Phlorizin; 1,5-6,8-9-Unknown.

, figureFileSmall=Aw26e0qEBP6J+Hd8E3WdIw==, figureFileBig=E/F5VsTaywilaZPU+/0y7Q==, tableContent=null), ArticleFig(id=1200147776987295937, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147771740222434, language=CN, label=图1, caption=冷水七样品与混合对照品超高效液相色谱(UPLC)图

2-儿茶素;3-表儿茶素;4-东莨菪素;7-根皮苷;1,5~6,8~9-未知。

, figureFileSmall=Aw26e0qEBP6J+Hd8E3WdIw==, figureFileBig=E/F5VsTaywilaZPU+/0y7Q==, tableContent=null), ArticleFig(id=1200147777176039624, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147771740222434, language=EN, label=Fig.2, caption=The UPLC chromatograms for the comparison of Impatientis Pritzelii Rhizoma and its adulterants

2-Catechin; 3-Epicatechin; 4-Scopolamine; 7-Phlorizin; 1,5-6,8-9-Unknown.

, figureFileSmall=4NsyDEssUBo5y+hkYsAN5w==, figureFileBig=+veElxnzjA8+O5IVfmdrdA==, tableContent=null), ArticleFig(id=1200147777264120009, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147771740222434, language=CN, label=图2, caption=冷水七与易混品UPLC对比图

2-儿茶素;3-表儿茶素;4-东莨菪素;7-根皮苷;1,5~6,8~9-未知。

, figureFileSmall=4NsyDEssUBo5y+hkYsAN5w==, figureFileBig=+veElxnzjA8+O5IVfmdrdA==, tableContent=null), ArticleFig(id=1200147777352200397, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147771740222434, language=EN, label=Fig.3, caption=Representative UPLC chromatograms for the comparison of 16 batches of Impatientis Pritzelii Rhizoma, figureFileSmall=E1uOmCRQAlAGGVP0C93TgA==, figureFileBig=V3GYgK1/UgLYcl0IdOCpag==, tableContent=null), ArticleFig(id=1200147777431892175, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147771740222434, language=CN, label=图3, caption=16批次冷水七样品UPLC色谱对比图, figureFileSmall=E1uOmCRQAlAGGVP0C93TgA==, figureFileBig=V3GYgK1/UgLYcl0IdOCpag==, tableContent=null), ArticleFig(id=1200147777524166865, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147771740222434, language=EN, label=Fig.4, caption=Representative HPLC chromatograms of phlorizin assay

A-phloridin reference substance; B-Impatientis Pritzelii Rhizoma sample.

, figureFileSmall=F2nkiootem/1cpxmItQkdg==, figureFileBig=2Oy7GWx+zZT+2t4lgylwrg==, tableContent=null), ArticleFig(id=1200147777620635861, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147771740222434, language=CN, label=图4, caption=根皮苷高效液相色谱(HPLC)图

A-根皮苷对照品;B-冷水七供试品。

, figureFileSmall=F2nkiootem/1cpxmItQkdg==, figureFileBig=2Oy7GWx+zZT+2t4lgylwrg==, tableContent=null), ArticleFig(id=1200147777712910552, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147771740222434, language=EN, label=Fig.5, caption=The UV absorption diagrams of phloridin by DAD

A-phloridin reference substance; B-Impatientis Pritzelii Rhizoma sample.

, figureFileSmall=lrNrNQD2ZENAbFh9GvOnCA==, figureFileBig=xileOmzKg8TRRY7bON8CgQ==, tableContent=null), ArticleFig(id=1200147777821962458, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147771740222434, language=CN, label=图5, caption=二极管阵列检测器(DAD)紫外吸收图

A-根皮苷对照品;B-冷水七供试品。

, figureFileSmall=lrNrNQD2ZENAbFh9GvOnCA==, figureFileBig=xileOmzKg8TRRY7bON8CgQ==, tableContent=null), ArticleFig(id=1200147777918431455, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147771740222434, language=EN, label=Fig.6, caption=The representative HPLC chromatograms for the determination of phlorizin in Impatientis Pritzelii Rhizoma and its adulterants, figureFileSmall=9XVui7RBA3wFADKSxn0Fcg==, figureFileBig=UD7XYFlgfRxU7woWEHj/YQ==, tableContent=null), ArticleFig(id=1200147778019094751, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147771740222434, language=CN, label=图6, caption=冷水七及其易混品中根皮苷含量测定HPLC色谱比较图, figureFileSmall=9XVui7RBA3wFADKSxn0Fcg==, figureFileBig=UD7XYFlgfRxU7woWEHj/YQ==, tableContent=null), ArticleFig(id=1200147778123952353, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147771740222434, language=EN, label=Tab.1, caption=

Sample list for 21 Batchs

, figureFileSmall=null, figureFileBig=null, tableContent=
No. Variety name Sample number Obtained time Obtained place(In Chinese)
1 Impatiens pritzelii LSQ20210125 20210125 Bajiao town, Jianshi county, Enshi city, Hubei province(湖北省恩施市建始县芭蕉乡)
2 I. pritzelii LSQ20210516 20210516 Shazhenxi town, Zigui county, Yichang city, Hubei province(湖北省宜昌市秭归县沙镇溪镇)
3 I. pritzelii LSQ20210624 20210624 Wuduhe town, Yiling district, Yichang city, Hubei province(湖北省宜昌市夷陵区雾渡河镇)
4 I. pritzelii LSQ20211115 20211115 Changyang county, Yichang city, Hubei province(湖北省宜昌市长阳县)
5 I. pritzelii LSQ20211119 20211119 Y041 road, Yangjiaping village, Badong county, Enshi city, Hubei province(湖北省恩施土家族苗族自治州巴东县杨家坪村Y041公路边)
6 I. pritzelii LSQ20211120 20211120 Near the entrance of Nanjinguan Grand Canyon, Yichang city, Hubei province(湖北省宜昌南津关大峡谷入口处附近)
7 I. pritzelii LSQ20230322 20230322 Zhushaxi village, Bajiao town, Enshicity, Hubei province(湖北省恩施市芭蕉乡朱砂溪村)
8 I. pritzelii LSQ20230324 20230324 Longtangou, Bajiao town, Enshi city, Hubei province (湖北省恩施市芭蕉乡龙潭沟)
9 I. pritzelii LSQ20230423 20230423 Houfengxi village, Longzhouping town, Changyang county, Yichang city, Hubei province(湖北省宜昌市长阳土家族自治县龙舟坪镇后峰溪村)
10 I. pritzelii LSQ20230423M 20230423M Houfengxi village, Longzhouping town, Changyang county, Yichang city, Hubei province(湖北省宜昌市长阳土家族自治县龙舟坪镇后峰溪村)
11 I. pritzelii LSQ20230531 20230531 Longjiabao, Badong county, Enshi city, Hubei province(湖北省恩施土家族苗族自治州巴东县龙家包)
12 I. pritzelii LSQ2023060101 2023060101 Tuobaziqiao, gezi river, Badong county, Enshi city, Hubei province(湖北省恩施土家族苗族自治州巴东县格子河拓跋子桥)
13 I. pritzelii LSQ2023060102 2023060102 Y041 road, Yangjiaping village, Badong county, Enshi city, Hubei province(湖北省恩施土家族苗族自治州巴东县杨家坪村Y041公路边)
14 I. pritzelii LSQ2023060103 2023060103 Baimaning village, Yesanguan town, Badong county, Enshi city, Hubei province(湖北省恩施土家族苗族自治州巴东县野三关镇白马岭村)
15 I. pritzelii LSQ20230602 20230602 Tigeryue stream, Nanjinguan valley, Yichang city, Hubei province(湖北省宜昌市南津关大峡谷虎跃溪)
16 I. pritzelii LSQ20230603 20230603 Qiujiashan village, Changyang county, Yichang city, Hubei province(湖北省宜昌长阳县邱家山村)
17 I. piufanensis KJ20211117 20211117 Zhanmahe nature reserve, Xianfeng county, Enshi city, Hubei province(湖北省恩施土家族苗族自治州咸丰县展马河自然保护区)
18 I. uliginosa DSJF20220613 20220613 Panlong river, Panlong district, Kunming city, Yunnan province(云南省昆明市盘龙区盘龙江边)
19 I. siculifer HJF202205271 20220527 Pingbian county, Honghe city, Yunnan province(云南省红河州屏边县)
20 I. siculifer HJF20220716 20220716 Fuer mountain, Bajiao town, Enshi city, Hubei province(湖北省恩施市芭蕉乡富耳山)
21 I. noli-tangere SJF20220803 20220803 Fusong county, Baishan city, Jilin province(吉林省白山市抚松县)
), ArticleFig(id=1200147778241392867, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147771740222434, language=CN, label=表1, caption=

21批样品信息表

, figureFileSmall=null, figureFileBig=null, tableContent=
No. Variety name Sample number Obtained time Obtained place(In Chinese)
1 Impatiens pritzelii LSQ20210125 20210125 Bajiao town, Jianshi county, Enshi city, Hubei province(湖北省恩施市建始县芭蕉乡)
2 I. pritzelii LSQ20210516 20210516 Shazhenxi town, Zigui county, Yichang city, Hubei province(湖北省宜昌市秭归县沙镇溪镇)
3 I. pritzelii LSQ20210624 20210624 Wuduhe town, Yiling district, Yichang city, Hubei province(湖北省宜昌市夷陵区雾渡河镇)
4 I. pritzelii LSQ20211115 20211115 Changyang county, Yichang city, Hubei province(湖北省宜昌市长阳县)
5 I. pritzelii LSQ20211119 20211119 Y041 road, Yangjiaping village, Badong county, Enshi city, Hubei province(湖北省恩施土家族苗族自治州巴东县杨家坪村Y041公路边)
6 I. pritzelii LSQ20211120 20211120 Near the entrance of Nanjinguan Grand Canyon, Yichang city, Hubei province(湖北省宜昌南津关大峡谷入口处附近)
7 I. pritzelii LSQ20230322 20230322 Zhushaxi village, Bajiao town, Enshicity, Hubei province(湖北省恩施市芭蕉乡朱砂溪村)
8 I. pritzelii LSQ20230324 20230324 Longtangou, Bajiao town, Enshi city, Hubei province (湖北省恩施市芭蕉乡龙潭沟)
9 I. pritzelii LSQ20230423 20230423 Houfengxi village, Longzhouping town, Changyang county, Yichang city, Hubei province(湖北省宜昌市长阳土家族自治县龙舟坪镇后峰溪村)
10 I. pritzelii LSQ20230423M 20230423M Houfengxi village, Longzhouping town, Changyang county, Yichang city, Hubei province(湖北省宜昌市长阳土家族自治县龙舟坪镇后峰溪村)
11 I. pritzelii LSQ20230531 20230531 Longjiabao, Badong county, Enshi city, Hubei province(湖北省恩施土家族苗族自治州巴东县龙家包)
12 I. pritzelii LSQ2023060101 2023060101 Tuobaziqiao, gezi river, Badong county, Enshi city, Hubei province(湖北省恩施土家族苗族自治州巴东县格子河拓跋子桥)
13 I. pritzelii LSQ2023060102 2023060102 Y041 road, Yangjiaping village, Badong county, Enshi city, Hubei province(湖北省恩施土家族苗族自治州巴东县杨家坪村Y041公路边)
14 I. pritzelii LSQ2023060103 2023060103 Baimaning village, Yesanguan town, Badong county, Enshi city, Hubei province(湖北省恩施土家族苗族自治州巴东县野三关镇白马岭村)
15 I. pritzelii LSQ20230602 20230602 Tigeryue stream, Nanjinguan valley, Yichang city, Hubei province(湖北省宜昌市南津关大峡谷虎跃溪)
16 I. pritzelii LSQ20230603 20230603 Qiujiashan village, Changyang county, Yichang city, Hubei province(湖北省宜昌长阳县邱家山村)
17 I. piufanensis KJ20211117 20211117 Zhanmahe nature reserve, Xianfeng county, Enshi city, Hubei province(湖北省恩施土家族苗族自治州咸丰县展马河自然保护区)
18 I. uliginosa DSJF20220613 20220613 Panlong river, Panlong district, Kunming city, Yunnan province(云南省昆明市盘龙区盘龙江边)
19 I. siculifer HJF202205271 20220527 Pingbian county, Honghe city, Yunnan province(云南省红河州屏边县)
20 I. siculifer HJF20220716 20220716 Fuer mountain, Bajiao town, Enshi city, Hubei province(湖北省恩施市芭蕉乡富耳山)
21 I. noli-tangere SJF20220803 20220803 Fusong county, Baishan city, Jilin province(吉林省白山市抚松县)
), ArticleFig(id=1200147778342056164, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147771740222434, language=EN, label=Tab.2, caption=

The content determination results of phloridin in Impatientis Pritzelii Rhizoma

, figureFileSmall=null, figureFileBig=null, tableContent=
No. Sample number Similarity Content of phloridin/%
1 LSQ20210125 0.901 0.014 4
2 LSQ20210516 0.840 0.037 9
3 LSQ20210624 0.852 0.065 9
4 LSQ20211115 0.925 0.016 1
5 LSQ20211119 0.815 0.016 6
6 LSQ20211120 0.924 0.085 2
7 LSQ20230322 0.891 0.011 1
8 LSQ20230324 0.866 0.018 0
9 LSQ20230423 0.877 0.052 1
10 LSQ20230423M 0.890 0.067 6
11 LSQ20230531 0.845 0.022 4
12 LSQ2023060101 0.933 0.016 9
13 LSQ2023060102 0.836 0.018 0
14 LSQ2023060103 0.899 0.007 3
15 LSQ20230602 0.861 0.017 7
16 LSQ20230603 0.920 0.013 5
), ArticleFig(id=1200147778438525162, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147771740222434, language=CN, label=表2, caption=

冷水七样品测定结果表

, figureFileSmall=null, figureFileBig=null, tableContent=
No. Sample number Similarity Content of phloridin/%
1 LSQ20210125 0.901 0.014 4
2 LSQ20210516 0.840 0.037 9
3 LSQ20210624 0.852 0.065 9
4 LSQ20211115 0.925 0.016 1
5 LSQ20211119 0.815 0.016 6
6 LSQ20211120 0.924 0.085 2
7 LSQ20230322 0.891 0.011 1
8 LSQ20230324 0.866 0.018 0
9 LSQ20230423 0.877 0.052 1
10 LSQ20230423M 0.890 0.067 6
11 LSQ20230531 0.845 0.022 4
12 LSQ2023060101 0.933 0.016 9
13 LSQ2023060102 0.836 0.018 0
14 LSQ2023060103 0.899 0.007 3
15 LSQ20230602 0.861 0.017 7
16 LSQ20230603 0.920 0.013 5
), ArticleFig(id=1200147778518216938, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147771740222434, language=EN, label=Tab.3, caption=

The results of correlation analysis between phlorizin and obtained time obtained place

, figureFileSmall=null, figureFileBig=null, tableContent=
Item Phlorizin
content		 Obtained
place		 Obtained
time
Similarity Pearson correlation 0.041 -0.183 0.161
Sig.(Two-tailed) 0.880 0.497 0.551
Number of cases 16 16 16
Phlorizin content Pearson - -0.6191) -0.104
correlation Sig.(Two-tailed) - 0.011 0.701
Number of cases - 16 16
), ArticleFig(id=1200147778618880237, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1200147771740222434, language=CN, label=表3, caption=

根皮苷含量与采集地采收时间相关性分析结果

, figureFileSmall=null, figureFileBig=null, tableContent=
Item Phlorizin
content		 Obtained
place		 Obtained
time
Similarity Pearson correlation 0.041 -0.183 0.161
Sig.(Two-tailed) 0.880 0.497 0.551
Number of cases 16 16 16
Phlorizin content Pearson - -0.6191) -0.104
correlation Sig.(Two-tailed) - 0.011 0.701
Number of cases - 16 16
)], attaches=null, journal=Journal(id=1190317596361715715, delFlag=0, nameCn=中国药学杂志, nameEn=Chinese Pharmaceutical Journal, nameHistory1=null, nameHistory2=null, issn=1001-2494, eissn=null, cn=11-2162/R, coden=null, periodic=3, language=CN, oaType=null, ccby=null, superviseOffice=null, ownerOffice=null, pubOffice=null, editorOffice=null, officeType=null, aims=null, clcCode=null, officeProv=null, officeCity=null, officeAddr=null, officeZip=null, officeEmail=null, officePhone=null, editDirector=null, officeDirector=null, officeDirectorPhone=null, officeStaffNum=null, officeEmpNum=null, coverPicUrl=hRN1R6HnoNwYkve/JRn0DA==, journalPrice=null, startedYear=null, abbrevIsoEn=null, journalRemark=null, publicationField=null, createdTime=1761723430007, updatedTime=1761735858241, createdBy=18614031015, updatedBy=13701087609, firstLetterCn=C, firstLetterEn=C, subjectCode=Life Sciences, subjectName=Life Sciences, subjectCodeEn=Life Sciences, subjectNameEn=null, picCn=hRN1R6HnoNwYkve/JRn0DA==, picEn=xSRntM4yOh2wVIE2w+OjYg==, jcr=null, cjcr=null, exts=[JournalExt(id=1190369724262355196, language=CN, name=中国药学杂志, nameHistory1=null, nameHistory2=null, managedBy=, sponsoredBy=, publishedBy=, editorOffice=, officeProv=null, officeCity=null, officeAddr=, officeZip=, editDirector=, officeDirector=null, officePhone=null, coverPicUrl=null, journalRemark=, submitArticleUrl=null, websiteUrl=, createdTime=1761735858264, updatedTime=1761735858264, createdBy=13701087609, updatedBy=13701087609, submissionGuidelinesUrl=, submissionAuthorUrl=https://zgyxzzauthor.manuscriptcloud.com/login, submissionEditorUrl=https://zgyxzzeditor.manuscriptcloud.com/login, submissionReviewUrl=https://zgyxzzauthor.manuscriptcloud.com/login, submissionCeEditorUrl=, submissionAeEditorUrl=, option={"copyright":""}), JournalExt(id=1190369724358824189, language=EN, name=Chinese Pharmaceutical Journal, nameHistory1=null, nameHistory2=null, managedBy=, sponsoredBy=, publishedBy=, editorOffice=, officeProv=null, officeCity=null, officeAddr=, officeZip=, editDirector=, officeDirector=null, officePhone=null, coverPicUrl=null, journalRemark=, submitArticleUrl=null, websiteUrl=, createdTime=1761735858287, updatedTime=1761735858287, createdBy=13701087609, updatedBy=13701087609, submissionGuidelinesUrl=, submissionAuthorUrl=https://zgyxzzauthor.manuscriptcloud.com/login, submissionEditorUrl=https://zgyxzzeditor.manuscriptcloud.com/login, submissionReviewUrl=https://zgyxzzauthor.manuscriptcloud.com/login, submissionCeEditorUrl=, submissionAeEditorUrl=, option={"copyright":""})], databaseList=null, tenantJournalId=1190317699101192196, websiteList=[Website(id=1190317834875011552, webName=null, webTitle=null, webDomain=null, webCopyrigh=null, webIpcNo=null, seoTitle=null, seoKeywords=null, seoDescription=null, tenantJournalId=null, journalId=1190317699101192196, journalNameCn=null, journalNameEn=null, grayFlag=null, tenantId=1146029695717560320, platformId=null, journalGroupId=null, journalGroupNameCn=null, journalGroupNameEn=null, type=1, domain=https://castjournals.cast.org.cn/joweb/zgyxzz/CN, language=CN, createTime=1761723486870, createBy=18614031015, updateTime=1761723510130, updateBy=18614031015, name=中国药学杂志-中文, tplId=1146099689490845704, title=中国药学杂志, delFlag=0, indexPage=/home, props=[WebsiteProps(id=1190318144041353703, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1190317834875011552, code=articleTextType, value=kx, createTime=1761723560581, updateTime=1761723560581, creator=18614031015, updator=18614031015), WebsiteProps(id=1190318144016187876, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1190317834875011552, code=banner, value=null, createTime=1761723560575, updateTime=1761723560575, creator=18614031015, updator=18614031015), WebsiteProps(id=1190318144062325226, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1190317834875011552, code=grayFlag, value=0, createTime=1761723560586, updateTime=1761723560586, creator=18614031015, updator=18614031015), WebsiteProps(id=1190318144007799267, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1190317834875011552, code=logo, value=https://castjournals.cast.org.cn/joweb/zgyxzz/CN/file/pic?fileId=puyAm9wIHqZks7K8hj8APQ==, createTime=1761723560573, updateTime=1761723560573, creator=18614031015, updator=18614031015), WebsiteProps(id=1190318144074908140, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1190317834875011552, code=minRunFlag, value=0, createTime=1761723560589, updateTime=1761723560589, creator=18614031015, updator=18614031015), WebsiteProps(id=1190318144032965094, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1190317834875011552, code=picServerUrl, value=https://castjournals.cast.org.cn/joweb/zgyxzz/CN/file/pic, createTime=1761723560579, updateTime=1761723560579, creator=18614031015, updator=18614031015), WebsiteProps(id=1190318144070713835, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1190317834875011552, code=silenceFlag, value=0, createTime=1761723560588, updateTime=1761723560588, creator=18614031015, updator=18614031015), WebsiteProps(id=1190318144024576485, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1190317834875011552, code=staticResourcePath, value=https://castjournals.cast.org.cn/joweb/cast_kjdb_cn_619/, createTime=1761723560577, updateTime=1761723560577, creator=18614031015, updator=18614031015), WebsiteProps(id=1190318144049742312, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1190317834875011552, code=themeColor, value=null, createTime=1761723560583, updateTime=1761723560583, creator=18614031015, updator=18614031015), WebsiteProps(id=1190318144053936617, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1190317834875011552, code=themeStyle, value=null, createTime=1761723560584, updateTime=1761723560584, creator=18614031015, updator=18614031015)]), Website(id=1190317834937926113, webName=null, webTitle=null, webDomain=null, webCopyrigh=null, webIpcNo=null, seoTitle=null, seoKeywords=null, seoDescription=null, tenantJournalId=null, journalId=1190317699101192196, journalNameCn=null, journalNameEn=null, grayFlag=null, tenantId=1146029695717560320, platformId=null, journalGroupId=null, journalGroupNameCn=null, journalGroupNameEn=null, type=1, domain=https://castjournals.cast.org.cn/joweb/zgyxzz/EN, language=EN, createTime=1761723486885, createBy=18614031015, updateTime=1761723527689, updateBy=18614031015, name=中国药学杂志-英文, tplId=1146101810881728533, title=Chinese Pharmaceutical Journal, delFlag=0, indexPage=/home, props=[WebsiteProps(id=1190318170478051825, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1190317834937926113, code=articleTextType, value=kx, createTime=1761723566884, updateTime=1761723566884, creator=18614031015, updator=18614031015), WebsiteProps(id=1190318170461274606, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1190317834937926113, code=banner, value=null, createTime=1761723566880, updateTime=1761723566880, creator=18614031015, updator=18614031015), WebsiteProps(id=1190318170494829044, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1190317834937926113, code=grayFlag, value=0, createTime=1761723566888, updateTime=1761723566888, creator=18614031015, updator=18614031015), WebsiteProps(id=1190318170452885997, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1190317834937926113, code=logo, value=https://castjournals.cast.org.cn/joweb/zgyxzz/EN/file/pic?fileId=puyAm9wIHqZks7K8hj8APQ==, createTime=1761723566878, updateTime=1761723566878, creator=18614031015, updator=18614031015), WebsiteProps(id=1190318170507411958, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1190317834937926113, code=minRunFlag, value=0, createTime=1761723566891, updateTime=1761723566891, creator=18614031015, updator=18614031015), WebsiteProps(id=1190318170473857520, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1190317834937926113, code=picServerUrl, value=https://castjournals.cast.org.cn/joweb/zgyxzz/EN/file/pic, createTime=1761723566883, updateTime=1761723566883, creator=18614031015, updator=18614031015), WebsiteProps(id=1190318170503217653, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1190317834937926113, code=silenceFlag, value=0, createTime=1761723566890, updateTime=1761723566890, creator=18614031015, updator=18614031015), WebsiteProps(id=1190318170465468911, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1190317834937926113, code=staticResourcePath, value=https://castjournals.cast.org.cn/joweb/cast_kjdb_en_623/, createTime=1761723566881, updateTime=1761723566881, creator=18614031015, updator=18614031015), WebsiteProps(id=1190318170482246130, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1190317834937926113, code=themeColor, value=null, createTime=1761723566885, updateTime=1761723566885, creator=18614031015, updator=18614031015), WebsiteProps(id=1190318170486440435, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1190317834937926113, code=themeStyle, value=null, createTime=1761723566886, updateTime=1761723566886, creator=18614031015, updator=18614031015)])], journalTitle=中国药学杂志, weixinUrl=null, journalUrl=http://www.zgyxzz.com.cn/, iacademicId=null, status=1, seqNo=null, journalTitleEn=Chinese Pharmaceutical Journal, journalPhotoCn=hRN1R6HnoNwYkve/JRn0DA==, journalPhotoEn=xSRntM4yOh2wVIE2w+OjYg==, journalFirstLetter=C, journalRecommend=null, journalNew=null, journalCollection=null, jcrJf=null, cjcrJf=null, jcrJfStr=null, cjcrJfStr=null, submissionFirstDecision=null, sciSubjectClassification=null, casSubjectClassification=null, citeScore=null, totalCitationFrequency=null, icpCode=null, psCode=null, advertisingLicenseCode=null, copyrightInformation=null, country=null, option=, provinceCode=null, provinceName=null, collectFlag=false), detailUrlCn=https://castjournals.cast.org.cn/joweb/zgyxzz/CN/10.11669/cpj.2024.09.008, detailUrlEn=https://castjournals.cast.org.cn/joweb/zgyxzz/EN/10.11669/cpj.2024.09.008, pdfUrlCn=https://castjournals.cast.org.cn/joweb/zgyxzz/CN/PDF/10.11669/cpj.2024.09.008, pdfUrlEn=https://castjournals.cast.org.cn/joweb/zgyxzz/EN/PDF/10.11669/cpj.2024.09.008, aliStartDate=null, aliEndDate=null, collectionFlag=false, citedCount=null, citedUrl=null, reference=null)
收藏切换
土家族药材冷水七指纹图谱及根皮苷含量测定的研究
收藏切换
PDF下载
肖凌 1 , 刘梦涵 2 , 徐梦麟 2 , 胡敏 1, * , 汤俊 2, *
中国药学杂志 | 论著 2024,59(9): 827-833
收起
收藏切换
中国药学杂志 | 论著 2024, 59(9): 827-833
土家族药材冷水七指纹图谱及根皮苷含量测定的研究
全屏
肖凌1, 刘梦涵2, 徐梦麟2, 胡敏1, *, 汤俊2, *
作者信息
  • 1 湖北省药品监督检验研究院, 国家药品监督管理局中药质量控制重点实验室, 湖北省药品质量检测与控制工程技术研究中心, 武汉 430075
  • 2 武汉大学药学院,组合生物合成与新药发现教育部重点实验室, 武汉 430071

    • 肖凌,女,副主任药师 研究方向:中药质量评价新技术新方法的研究及应用

通讯作者:

*汤俊,男,博士,副教授 研究方向:中药、天然药物化学成分及活性研究;
胡敏,女,硕士,主任药师 研究方向:药品质量分析 Tel:(027)87705232
Fingerprint and Phlorizin Content Assay of Tujia Ethnomedicinal Herb Impatientis Pritzelii Rhizoma
Ling XIAO1, Menghan LIU2, Menglin XU2, Min HU1, *, Jun TANG2, *
Affiliations
  • 1 NMPA Key Laboratory of Quality Control of Chinese Medicine Hubei, Engineering Research Center for Drug Quality Control, Hubei Institute for Drug Control, Wuhan 430075, China
  • 2 Key Laboratory of Combinatorial Biosynthesis and Drug Discovery (Wuhan University), Ministry of Education, School of Pharmaceutical Sciences, Wuhan University, Wuhan 430071, China
出版时间: 2024-05-08 doi: 10.11669/cpj.2024.09.008
文章导航
收藏切换
摘要
收起

目的 提高土家族药材冷水七的质量控制方法,建立该药材指纹图谱及含量测定方法。方法 指纹图谱采用超高效液相色谱法(UPLC),ACQUITY UPLC BEH C18柱,根皮苷含量测定采用高效液相色谱法(HPLC),Eclipse XDB-C18柱,流动相均为乙腈-0.1%甲酸梯度洗脱,检测波长为283 nm,对冷水七及其易混品(同属块节凤仙花、滇水金凤、黄金凤、水金凤的根茎)进行比较研究。结果 指纹图谱中9个特征峰可作为冷水七的特征峰,其中4个峰分别与儿茶素、表儿茶素、东莨菪素及根皮苷相对应;该指纹图谱反映了冷水七化学成分的分布,且可与其易混品区分。根皮苷为首次在冷水七中发现,16批冷水七中根皮苷含量范围为0.007%~0.085%,易混品未检出根皮苷。结论 本研究建立的冷水七指纹图谱及其根皮苷含量测定方法专属、准确、有效,可用于冷水七药材的质量控制。

冷水七  /  凤仙花属  /  根皮苷  /  指纹图谱

OBJECTIVE To establish the fingerprint and content determination method of Impatientis Pritzelii Rhizoma to improve the quality control method of Tujia ethnomedicinal herb Impatientis Pritzelii Rhizoma. METHODS The fingerprint was established by UPLC with ACQUITY UPLC BEH C18 column. Phlorizin content assay was performed by HPLC with Eclipse XDB-C18 column. The mobile phases for both methods were composed of acetonitrile and 0.1% formic acid with different gradient elution procedures. The detection wavelength was set at 283 nm. The comparative study was carried out between Impatientis Pritzelii Rhizoma and its adulterants (such as the roots and rhizomes of Impatiens pinfanensis, I. uliginosa, I. siculifer and I. noli-tangere). RESULTS Nine main chromatographic peaks in the fingerprint could be served as the characteristic peaks of Impatientis Pritzelii Rhizoma, four of which were corresponding to catechin, epicatechin, scopolamine and phlorizin, respectively. The established fingerprint could reflect the distribution of the chemical components of Impatientis Pritzelii Rhizoma, which was distinguished from four adulterants. Phlorizin was found and determined in Impatientis Pritzelii Rhizoma for the first time. The contents in 16 batches of Impatientis Pritzelii Rhizoma ranged from 0.007% to 0.085%, while no phloridin was found in the adulterants. CONCLUSION The established methods for the fingerprint and phlorizin content determination of Impatientis Pritzelii Rhizoma are specific, accurate and effective, which could be used for its quality control.

Impatientis Pritzelii Rhizoma  /  Impatiens  /  phlorizin  /  fingerprint
肖凌, 刘梦涵, 徐梦麟, 胡敏, 汤俊. 土家族药材冷水七指纹图谱及根皮苷含量测定的研究. 中国药学杂志, 2024 , 59 (9) : 827 -833 . DOI: 10.11669/cpj.2024.09.008
Ling XIAO, Menghan LIU, Menglin XU, Min HU, Jun TANG. Fingerprint and Phlorizin Content Assay of Tujia Ethnomedicinal Herb Impatientis Pritzelii Rhizoma[J]. Chinese Pharmaceutical Journal, 2024 , 59 (9) : 827 -833 . DOI: 10.11669/cpj.2024.09.008
正文
收起
冷水七来源为凤仙花科植物湖北凤仙花(Impatiens pritzellii Hook. f. var. hupehensis Hook. f.)的根及根茎,是土家族治疗类风湿关节炎常用的要药,具有祛风除湿、散瘀消肿、镇痛止血和清热解毒的功效,药理活性主要表现为镇痛、抗炎以及抗类风湿作用等[1-2]。目前已经从冷水七中分离鉴定了41个化合物,主要包括三萜皂苷类、黄酮类和香豆素类等成分[3-7]。2018年版《湖北省中药材标准》[8]收载了冷水七的质量标准,包括性状、显微、薄层等质量控制指标,文献[9]也仅有生药学研究,缺乏整体质量控制与代表性成分定量指标。为提高冷水七药材的品质评价方法,从而完善其质量标准,本研究从冷水七化学成分谱入手,将同属易混品种进行对比研究,建立了专属性较好的指纹图谱,进而对其中代表性成分根皮苷进行了含量测定方法研究。
1 仪器与试剂
收起
Waters ACQUITY UPLC系统、Waters 600型高效液相色谱仪(美国Waters集团);Agilent 1260型高效液相色谱仪(美国安捷伦科技有限公司);岛津LC-20A型高效液相色谱仪(日本岛津制作所);UltiMate3000型戴安高效液相色谱仪(美国戴安公司);XP204型万分之一电子天平(瑞士Mettler公司);LC-350A型超声波仪(济宁市中区鲁超仪器厂);Milipore-Q Reference system超纯化水机(美国Milipore公司)。
儿茶素(批号:110877-202005,含量以95.1%计)、表儿茶素(批号:110878-201703,含量以99.7%计)(中国食品药品检定研究院);东莨菪素(CAS号92-61-5,含量>98%,成都普思生物科技有限公司);根皮苷(CAS号60-81-1,含量>98%,自制)。甲醇、乙腈均为色谱纯。
共采集样品21批,其中16批冷水七为湖北凤仙花(Impatiens pritzelii Hook. f.)的根及根茎,同属近缘品种的根及根茎包括,块节凤仙花(I. piufanensis J. D. Hooker)1批、滇水金凤(I. uliginosa Franch.)1批、黄金凤(I. siculifer Hook. f.)2批、水金凤(I. noli-tangere L.)1批,样品信息见表1。所有样品均由武汉大学植物标本馆杜巍博士鉴定。
2 方法与结果
收起
2.1 超高效液相色谱(UPLC)指纹图谱的建立
2.1.1 色谱条件
Waters UPLC H-Class,ACQUITY UPLC BEH C18柱 (2.1 mm×100 mm,1.7 μm),流动相乙腈(A)-0.1%甲酸(B)梯度洗脱(0~13 min,5%~10%A;13~20 min,10%~20%A;20~30 min,20%~35%A;30~32 min,35%~50%A;32~35 min,50%~5%A;35~38 min,5% A),流速0.2 mL·min-1;二极管阵列检测器(DAD)检测器,波长283 nm;柱温30 ℃;进样体积2 μL。
2.1.2 对照品溶液的配制
分别精密称定儿茶素、表儿茶素、东莨菪素、根皮苷对照品适量,加甲醇制成质量浓度分别为15、30、50、50 μg·mL-1的混合对照品溶液。
2.1.3 供试品溶液的制备
取干燥的冷水七药材粉末(过三号筛)2.0 g,精密称定,置250 mL具塞锥形瓶中,精密加入体积分数90%甲醇25 mL,称定质量,加热回流提取45 min,放冷,称定质量,用体积分数90%甲醇补足减失的质量,摇匀,滤过,取续滤液,即得。
2.1.4 对照图谱的生成
将冷水七样品色谱图导入中药色谱指纹图谱相似度评价系统2010版,采用中位数法,时间窗宽度设置为0.2,生成冷水七对照图谱,冷水七样品及混合对照品色谱图见图1,冷水七与易混品色谱叠加图见图2,可看出此条件下冷水七指纹图谱可与其他品种明显区分,选择分离度较好的9个主要色谱峰为特征鉴别峰,其中4个峰能与儿茶素、表儿茶素、东莨菪素、根皮苷对照品相对应。
2.1.5 方法学考察
精密度:取供试品溶液,按“2.1.1”项下色谱条件,连续进样6次,结果9个主要色谱峰的相对保留时间的相对标准偏差(RSD)值为0.02%~1.68%。表明仪器精密度良好,符合指纹图谱的要求。
稳定性:取供试品溶液,分别在6个时间点(0、2、4、8、12、24 h)进样测定,结果9个主要色谱峰的相对保留时间的RSD值为0.03%~1.89%。表明方法的稳定性较好,符合指纹图谱的要求。
重复性:平行制备6份供试品进行重复性测定,结果9个主要色谱峰的相对保留时间的RSD值为0.03%~2.89%;表明方法的重复性较好,符合指纹图谱的要求。
耐用性:采用3种不同的色谱柱进样分析,ACQUITY UPLC BEH C18(2.1 mm×100 mm,1.7 μm);YMC-Triart C18(2.1 mm×100 mm,1.9 μm);ACQUITY UPLC BEH Shield RP18(2.1 mm×100 mm,1.7 μm),结果9个主要色谱峰的相对保留时间RSD值为1.05%~4.89%,证明该方法耐用性较好。
2.1.6 相似度计算
对各批次冷水七样品以9个特征峰进行匹配计算相似度,结果见表2,相似度为0.84~0.93,不同批次冷水七的典型色谱叠加图见图3。
2.2 根皮苷含量测定
2.2.1 色谱条件
安捷伦1260型高效液相色谱仪,色谱柱为Eclipse XDB-C18(4.6 mm×250 mm,5 μm),流动相乙腈(A)-0.1%甲酸(B)梯度洗脱(0~30 min,18%A;30~32 min,18%→60%A;32~35 min,60%A,35~37 min,60%→18%A,37~42 min,18%A),流速为1 mL·min-1,柱温30 ℃,DAD检测器,检测波长283 nm,柱温为30 ℃,进样体积为10 μL。
2.2.2 对照品溶液制备
精密称定,根皮苷对照品5.11 mg,加50 mL甲醇制成质量浓度为102.20 μg·mL-1的溶液,作为根皮苷对照品储备液。
2.2.3 供试品溶液制备
取冷水七药材粉末(过三号筛)0.5 g,精密称定,置250 mL具塞锥形瓶中,精密加入体积分数90%甲醇25 mL,称定质量,加热回流提取45 min,放冷,称定质量,用体积分数90%甲醇补足减失的质量,摇匀,滤过,取续滤液,即得。
2.2.4 方法学考察
专属性:对照品及供试品的色谱图及DAD紫外吸收图见图4~5,结果可看出,供试品峰分离度较好,紫外吸收图与对照品一致,此条件下供试品测定无干扰。
线性关系:精密吸取根皮苷对照品储备液0.5、1、2.5、5、10、25 mL于50 mL量瓶中,以甲醇定容至刻度,摇匀。分别进样5 μL,按上述确定的色谱条件测定峰面积,以浓度作为横坐标,峰面积为纵坐标进行线性回归分析,得到线性回归方程为y=105 53x-3 079.6,相关系数r=0.999 9,结果表明,根皮苷对照品在1.022~102.2 μg·mL-1范围内线性较好。
精密度:取样品编号为LSQ20211115供试品溶液,连续进样6次,计算根皮苷峰面积,RSD值为1.04%,证明仪器精密度良好。
稳定性:取样品编号为LSQ20211115供试品溶液分别在6个时间点(0、2、4、8、12、24 h)进行测定,计算根皮苷峰含量,RSD值为1.82%,表明供试品溶液在24 h内基本稳定。
重复性:取样品编号为LSQ20211115样品6份,按供试品制备方法制备,分别依照色谱条件进样分析,计算根皮苷峰含量为0.016 1%,RSD值为1.46%,表明该方法重复性良好。
准确度:减半称取样品编号为LSQ20211115供试品,1∶1加入对照品,按“2.2.3”项下制备准确度供试品6份,分别依照色谱条件进样分析,计算根皮苷回收率的RSD值,结果根皮苷平均回收率为95.8%,RSD值为3.25%,表明方法的准确度较好。
范围重复性和准确度:依照确定方法,分别制备0.5倍与1.5倍取样时的各3份测定重复性,计算根皮苷峰含量为0.016 7%,RSD值为1.16%,表明范围重复性较好;再按0.5倍和1.5倍取样量的各3份准确度样品分别按1∶1加入对照品,按“2.2.3”项下制备、测定,范围准确度测得的平均回收率分别为92.78%和101.33%,RSD值分别为2.79%和2.28%,表明范围准确度较好。
耐用性:采用3种不同的仪器及色谱柱进行耐用性试验,分别为岛津LC-20A型液相色谱仪,色谱柱Eclipse XDB-C18(4.6 mm×250 mm,5 μm);安捷伦1260型液相色谱仪,色谱柱Xbridge C18(4.6 mm×250 mm,5 μm);戴安U3000型液相色谱仪色谱柱SPODS-A C18(4.6 mm×250 mm,5 μm),含量均值为0.151 9 mg·g-1,RSD为1.90%,证明该方法耐用性较好。
2.3 样品测定
2.3.1 相似度及含量测定结果
21批样品按上述条件进行测定,结果见图6,从谱图结果可知易混品在根皮苷处无相应色谱峰出现,16批冷水七样品根皮苷含量测定及相似度结果见表2。根据表2的计算结果可以看出,按照该方法测定的16批冷水七中根皮苷含量范围为0.007%~0.085%,其中,样品LSQ202300103含量最低为0.007 3%,样品LSQ 20211120含量最高为0.085 2%,平均含量为0.030%;产于恩施地区样品的含量范围为0.007 3%~0.022 4%,产于宜昌地区的含量范围为0.013 5%~0.085 2%。
2.3.2 结果分析
将相似度结果、根皮苷含量、采集地、采收时间,四者做相关性分析,结果见表3。采收时间与根皮苷含量及相似度相关性均不显著,而采集地对相似度影响不显著、对根皮苷含量有一定的影响,其中,产地为宜昌地区的含量总体高于产地为恩施地区的含量,推测可能与两地的地理环境差异有一定关系。
3 讨论
收起
为了全面反映冷水七所含化学成分,本研究在UPLC指纹图谱分析时采用了DAD联用MS检测器。在190~400 nm进行全波长扫描,选择了215、230、283、298、330 nm波长进行比较,结果在215和230 nm波长下的后半段基线波动较大,前半段峰形及分离度较差,283、298与330 nm下的色谱图峰分布较为合适,基线较平,且对比之下283 nm下的图中峰数目更多,峰响应强度更大,故将283 nm作为指纹图谱检测波长;通过MS信息及与对照品比对归属了4个特征峰,分别为儿茶素、表儿茶素、东莨菪素、根皮苷,其他5个特征峰通过紫外光谱及质谱信息,仍无法确认归属。另外UPLC指纹图谱在保留时间30 min之后,283 nm波长下无明显峰,但在 215 nm波长及MS检测下,有明显的吸收峰,经检测可能为以刺囊酸为苷元的系列刺囊酸皂苷[13],此部分仍有待进一步研究。
根皮苷是根皮素经糖基化后得到的衍生物,化学名为1-[2-(β-D-吡喃葡萄糖氧基)-4, 6-二羟基苯基]-3-(4-羟基苯基)-丙酮,属于黄酮类化合物中较为少见的二氢查尔酮苷。根皮苷具有多种药理活性,如抗氧化、降血糖、抗炎、抗肿瘤等,是一种天然来源并具有良好抗氧化和抗衰老活性的小分子化合物,具有较好的开发利用前景[10-12]。前期对冷水七的化学成分文献进行调研,尚未见冷水七中含有根皮苷的报道,笔者经过质谱解析和与对照品比对,确证了冷水七中含有根皮苷,且未见于其同属近缘种及易混品(块节凤仙花、滇水金凤、黄金凤和水金凤)中,进而对其含量测定方法进行了研究。
总之,本研究采用指纹图谱技术对冷水七药材整体质量进行了分析,建立的指纹图谱较能全面反映冷水七化学成分的分布;其次,首次在冷水七中发现了根皮苷,并建立了其含量测定方法。所建立的指纹图谱及根皮苷含量测定方法专属、准确,可区分冷水七易混品及有效地控制药材质量,该结果为冷水七进一步利用和开发提供了实验依据。
基金
收起
  • 湖北省重点研发计划项目“湖北中药质控标准物质研制及技术平台建立”资助(2020ACA007)
  • 国家药品监督管理局注册司民族药专项“特色民族药检验方法的示范性研究”资助(NIFDC-TCM2021-049-MZY011)
文献
收起
参考文献 引证文献
排序方式：
[1]
ZHANG J C. Study on anti-rheumatoid active ingredients of Lengshuiqi and anti-tumor components of Dianthi Herba[D]. Wuhan: Hubei University of Chinese Medicine, 2017.
[2]
LEI G L, WEI M Y, GUO H J, et al. Research and utilization of ethnomedicine“Lengshuiqi”[J]. Lishizhen Med Mater Med Res(时珍国医国药), 2012, 23(5):1272-1273.
[3]
ZHANG J C, HE W J, LIU Y W, et al. Chemical constituents from Impatiens pritzllii var. hupehensis[J]. China J Chin Mater Med(中国中药杂志), 2016, 41(1):83-86.
[4]
ZHOU X F, ZHAO X Y, TANG L, et al. Three new triterpenoid saponins from the rhizomes of Impatiens pritzellii var. Hupehensis[J]. J Asian Nat Prod Res, 2007, 9(4):379-385.
[5]
ZHOU X F. Material foundation study of Rhizoma Impatiens pritzellii and Radix Actinidia chinensis[D]. Wuhan: Huazhong University of Science and Technology, 2009.
[6]
ZHAOX Y, SUN H D, WU J Z. Studies on chemical constituents from rhizome of Impatiens pritzellii var. hupehensis[J]. China J Chin Mater Med(中国中药杂志), 2005, 30(8):584-586.
[7]
WEN D J. Research progress of Tujia medicine“Lengshuiqi”[J]. J Med Pharm Chin Minor(中国民族医药杂志), 2008, 14(5):62-64.
[8]
Medical Products Administration of Hubei Province, Quality Standard of Chinese Crude Drugs in Hubei Province(湖北省中药材质量标准)[M]. Beijing: China Medical Science and Technology Press, 2018:112.
[9]
XU R, WANG J, WAN D R. Study on quality standard of ethnomedicine“Lengshuiqi”[J]. J South-Cent Univ Natl(Nat Sci Ed)(中南民族大学学报自然科学版), 2009, 28(1):51-54.
[10]
HERRERA-GONZÁLEZ A, NÚÑEZ-LÓPEZ G, NÚÑEZ-DALLOS N, et al. Enzymatic synthesis of phlorizin fructosides[J]. Enzyme Microb Technol, 2021, 147:109783. DOI: 10.1016/j.enzmictec.2021.109783.
[11]
ONGAY K K, GRANATO D, BARRETO G E. Comparison of antioxidant capacity and network pharmacology of phloretin and phlorizin against neuroinflammation in traumatic brain injury[J]. Molecules, 2023, 28(3):919. DOI: 10.3390/molecules28030919.
[12]
HABTEMARIAM S. The molecular pharmacology of phloretin: anti-inflammatory mechanisms of action[J]. Biomedicines, 2023, 11(1):143. DOI: 10.3390/biomedicines11010143.
[13]
LIU M H, XIAO L, TANG J. Excavation of saponins components in Lengshuiqi by UPLC-Q-TOF-MS[C]// CCS 14th National Symposium on Natural Organic Chemistry. Wuhan: Chinese Chemical Society, 2023, 225-226.
2024年第59卷第9期
PDF下载
130
60
引用本文
BibTeX
文章信息
doi: 10.11669/cpj.2024.09.008
  • 接收时间：2023-11-06
  • 首发时间：2025-11-25
  • 出版时间：2024-05-08
补充材料
相关文章
文章信息
作者
出版历史
  • 收稿日期：2023-11-06
基金
湖北省重点研发计划项目“湖北中药质控标准物质研制及技术平台建立”资助(2020ACA007)
国家药品监督管理局注册司民族药专项“特色民族药检验方法的示范性研究”资助(NIFDC-TCM2021-049-MZY011)
作者信息
    1 湖北省药品监督检验研究院, 国家药品监督管理局中药质量控制重点实验室, 湖北省药品质量检测与控制工程技术研究中心, 武汉 430075
    2 武汉大学药学院,组合生物合成与新药发现教育部重点实验室, 武汉 430071

通讯作者:

*汤俊,男,博士,副教授 研究方向:中药、天然药物化学成分及活性研究;
胡敏,女,硕士,主任药师 研究方向:药品质量分析 Tel:(027)87705232
参考文献
分享链接
https://castjournals.cast.org.cn/joweb/zgyxzz/CN/10.11669/cpj.2024.09.008
分享至
全文二维码

扫描看全文

引用本文
BibTeX
本文的引用情况
2种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
关闭全屏
相关链接
论文
最新文章
热读文章
热点专题
内参
蓝皮书
产业发展报告
传播力报告
期刊分级目录
资讯
学术新闻
行业新闻
学术会议
行业会议
关于
关于我们
联系我们
北京市海淀区学院南路86号
100081
010-62199257
qkjq@cast.org.cn

中国科学技术协会版权所有 京ICP 备10216604号-4 海淀分局备案 1101084647
科技导报社主办 中国科协信息中心技术支持