Article(id=1190375274500952551, tenantId=1146029695717560320, journalId=1190317699101192196, issueId=1190375270847710190, articleNumber=1001-2494(2025)03-0244-06, orderNo=null, doi=10.11669/cpj.2025.03.006, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=research-article, receivedDate=1715011200000, receivedDateStr=2024-05-07, revisedDate=null, revisedDateStr=null, acceptedDate=null, acceptedDateStr=null, onlineDate=1761737181544, onlineDateStr=2025-10-29, pubDate=1738944000000, pubDateStr=2025-02-08, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1761737181544, onlineIssueDateStr=2025-10-29, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1761737181544, creator=13701087609, updateTime=1761737181544, updator=13701087609, issue=Issue{id=1190375270847710190, tenantId=1146029695717560320, journalId=1190317699101192196, year='2025', volume='60', issue='3', pageStart='209', pageEnd='312', issueExtLink='null', onlineDate='null', pubDate='null', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1761737180673, creator=13701087609, updateTime=1761793989024, updator=13701087609, preIssue=null, nextIssue=null, ext={EN=IssueExt(id=1190613542412890252, tenantId=1146029695717560320, journalId=1190317699101192196, issueId=1190375270847710190, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1190613542412890253, tenantId=1146029695717560320, journalId=1190317699101192196, issueId=1190375270847710190, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null}, startPage=244, endPage=249, ext={EN=ArticleExt(id=1190375274756805096, articleId=1190375274500952551, tenantId=1146029695717560320, journalId=1190317699101192196, language=EN, title=Identification and Analysis of Bacillus Species Contaminating Traditional Chinese Medicine Based on gyrB Gene, columnId=null, journalTitle=Chinese Pharmaceutical Journal, columnName=null, runingTitle=null, highlight=null, articleAbstract=

OBJECTIVE To indentify and analyze Bacillus species and their characteristics contaminating traditional Chinese medicine. METHODS Screening for a reaction system more suitable for gyrB gene amplification conditions and sequencing Bacillus species in contaminated traditional Chinese medicine preparations, medicinal herbal, herbal pieces and extracts. Analyzing the types and homogeneity of contaminating bacteria through comparing gene sequences and constructing a phylogenetic tree. RESULTS The results indicate that the prevalence of Bacillus subtilis as the most common contaminating bacterium across various sample types, followed by Bacillus velezensis, Bacillus megaterium, Bacillus licheniformis and Bacillus amyloliquefaciens. Additionally, pathogenic bacterium Bacillus cereus was also detected in this analysis. The gyrB gene demonstrates good discriminatory power for various Bacillus species and subspecies, effectively distinguishing same contaminating strains relationships among diverse production enterprises, showing relevance of sources. CONCLUSION To ensure the quality and safety of traditional Chinese medicine products, production enterprises must enhance control measures at the source during the production process and select appropriate sterilization processes tailored to the characteristics of traditional Chinese medicine varieties.

, correspAuthors=Manman LU, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, authorCompany=null, fund=null, authors=null, authorsList=Yongqi GAN, Cheng LIU, Bin ZHU, Lanyan FAN, Jun NONG, Jie HUANG, Wenchao LING, Manman LU), CN=ArticleExt(id=1190375463844418180, articleId=1190375274500952551, tenantId=1146029695717560320, journalId=1190317699101192196, language=CN, title=基于gyrB基因鉴定分析污染中药的芽孢杆菌, columnId=1190352405612040510, journalTitle=中国药学杂志, columnName=论著, runingTitle=null, highlight=null, articleAbstract=

目的 鉴定分析污染中药的芽孢杆菌种类。方法 筛选更适用于gyrB基因扩增条件的反应体系,并对污染中药制剂、中药材、饮片和中药提取物的芽孢杆菌进行测序,通过对比基因序列和构建系统发育树分析污染菌的种类和同源性。结果 枯草芽孢杆菌是各类样品中最常见的污染菌,其次为贝莱斯芽孢杆菌、巨大芽孢杆菌、地衣芽孢杆菌和解淀粉芽孢杆菌等,并检出条件致病菌蜡样芽孢杆菌;gyrB基因对芽孢杆菌各种、亚种具有较好的分辨力,能够有效区分同一种污染菌在不同生产企业间的菌株亲缘关系,并呈现来源的相关性。结论 为保证中药产品的质量和安全性,生产企业需加强从生产过程的源头控制原辅料带来的污染,根据中药品种的特性选择适宜的灭菌工艺。

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甘永琦,男,硕士,副主任药师 研究方向:生物检验及研究

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Food Drug(食品与药品), 2022, 24(2): 152-158., articleTitle=Study on effect of ⁶⁰Co-γ radiation sterilization on effective components of Shaoshangling Tincture based on HPLC fingerprints, refAbstract=null), Reference(id=1190958865056416221, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1190375274500952551, doi=null, pmid=null, pmcid=null, year=2021, volume=23, issue=6, pageStart=1029, pageEnd=1035, url=null, language=null, rfNumber=[33], rfOrder=32, authorNames=DING W, HE J, HUANG D P, journalName=Mod Chin Med (中国现代中药), refType=null, unstructuredReference=DING W, HE J, HUANG D P, et al. Study on effect of ⁶⁰Co-γ radiation sterilization on active ingredients of Polygoni Cuspidati Rhizoma et Radix based on HPLC fingerprint[J]. 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M-2 000 bp marker; 1-3-TB1, TB2 and negative control using F8 PCR master mix; 4-6-TB1, TB2 and negative control using PrimeSTAR max DNA polymerase; 7-9-TB1, TB2 and negative control using Q5 PCR master mix; 10-12-TB1, TB2 and negative control using platinu Ⅱ PCR master mix; 13-15-Amplification of 16S rRNA genes of TB1, TB2, and negative control using F8 PCR master mix.

, figureFileSmall=FUijvjuPj/XWvqUFqOYflA==, figureFileBig=NJzULRC3TSKoOjeK0qiwoA==, tableContent=null), ArticleFig(id=1190958862221066674, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1190375274500952551, language=CN, label=图1, caption=不同品牌聚合酶链式反应(PCR)预混液扩增gyrB基因的电泳结果

M-2 000 bp marker;1~3-F8预混液的TB1、TB2和阴性对照;4~6-PrimeSTAR预混液的TB1、TB2和阴性对照;7~9-Q5预混液的TB1、TB2和阴性对照;10~12-Platinu Ⅱ预混液的TB1、TB2和阴性对照;13~15-F8预混液扩增TB1、TB2和阴性对照的16S rRNA基因样品。

, figureFileSmall=FUijvjuPj/XWvqUFqOYflA==, figureFileBig=NJzULRC3TSKoOjeK0qiwoA==, tableContent=null), ArticleFig(id=1190958862304952755, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1190375274500952551, language=EN, label=Fig.2, caption=Electrophoretic results of adding Platinum GC Enhancer to amplify gyrB gene

M-2 000 bp marker; 1-3-TB1, TB2 and negative control without GC enhancer; 4-6-TB1, TB2 and negative control containing GC enhancer.

, figureFileSmall=IbTC8Jb2QdkE1UlDD/YXpA==, figureFileBig=AvVIIhMe92V6viVOuFECzA==, tableContent=null), ArticleFig(id=1190958862397227444, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1190375274500952551, language=CN, label=图2, caption=加入Platinum GC enhancer扩增gyrB基因的电泳结果

M-2 000 bp marker;1~3-不含GC增强剂的TB1、TB2和阴性对照;4~6-含GC增强剂的TB1、TB2和阴性对照。

, figureFileSmall=IbTC8Jb2QdkE1UlDD/YXpA==, figureFileBig=AvVIIhMe92V6viVOuFECzA==, tableContent=null), ArticleFig(id=1190958862455947701, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1190375274500952551, language=EN, label=Fig.3, caption=Distribution of Bacillus species contaminating traditional Chinese medicine, figureFileSmall=wNH9//ALVtvphWYInket7g==, figureFileBig=rrNK/Rwjuej5xIbWMGiTbQ==, tableContent=null), ArticleFig(id=1190958862514667958, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1190375274500952551, language=CN, label=图3, caption=污染中药的芽孢杆菌分布, figureFileSmall=wNH9//ALVtvphWYInket7g==, figureFileBig=rrNK/Rwjuej5xIbWMGiTbQ==, tableContent=null), ArticleFig(id=1190958862573388215, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1190375274500952551, language=EN, label=Fig.4, caption=Phylogenetic tree analysis of gyrB gene in different strains

The first letter S in the strain number-Shihu Yeguang Wan, T-Tianwang Buxin Wan, G-Guanmaining Preparation, X-Watermelon frost, and C-Traditional Chinese herbal pieces; The second letter represents the abbreviation of the production enterprise; Numbers represent different strains of bacteria.

, figureFileSmall=exwnqr+R4iLuFH1mN0meIw==, figureFileBig=USNiWlMLVjzBmSC2uHip+w==, tableContent=null), ArticleFig(id=1190958862644691384, tenantId=1146029695717560320, journalId=1190317699101192196, articleId=1190375274500952551, language=CN, label=图4, caption=不同菌株的gyrB基因进化树分析

菌株编号中第一个字母S-石斛夜光丸、T-天王补心丸、G-冠脉宁制剂、X-西瓜霜、C-中药饮片;第二个字母代表生产企业简称;数字代表不同菌株。

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