中国畜牧杂志

和田羊ESR2基因启动子克隆分析及其活性分析

PDF下载

中国畜牧杂志 | 畜牧生物技术 2026,62(2): 361-367

收起

中国畜牧杂志 |畜牧生物技术 2026 , 62 (2) : 361 -367

和田羊ESR2基因启动子克隆分析及其活性分析

全屏

谷若怀, 黄巧艳, 李伟, 孙慧萍, 朱乐潇, 邢凤,

作者信息

通讯作者:

邢凤

作者简介:

谷若怀（1999-），男，黑龙江鹤岗人，在读硕士研究生，主要从事动物繁殖育种研究，E-mail:a18346865515@163.com;

Affiliations

出版时间: 2026-02-10 doi: 10.19556/j.0258-7033.20250328-02

文章导航

摘要

收起

雌激素受体β基因（Estrogen Receptor Beta,ESR2）参与了绵羊的卵泡发育，但其调控机理尚不清楚。本研究以和田羊为试验对象，以NCBI数据库中绵羊ESR2基因启动子区2 871 bp序列为模板进行克隆测序，通过在线软件预测ESR2启动子区转录因子结合位点、CpG岛和调控元件，并以ESR2基因启动子区2 871 bp序列为模板构建6个连续缺失的荧光素酶载体，利用双荧光素酶报告基因系统筛选ESR2核心启动子区。本研究成功克隆了和田羊ESR2基因启动子区2 871 bp的序列，通过软件预测发现，和田羊ESR2基因启动子区存在4个潜在的转录起始位点、67个转录因子结合位点、1个TATA-box以及2个CpG岛。双荧光素酶活性检测结果表明ESR2基因启动子区位于L7(-527～-156)。本文研究结果为进一步探究绵羊ESR2基因启动子对绵羊卵泡发育的调控提供了新线索。

关键词

ESR2 / 和田羊 / 启动子 / 序列分析 / 核心启动子

引用本文

谷若怀, 黄巧艳, 李伟, 孙慧萍, 朱乐潇, 邢凤,. 和田羊ESR2基因启动子克隆分析及其活性分析. 中国畜牧杂志, 2026 , 62 (2) : 361 -367 . DOI: 10.19556/j.0258-7033.20250328-02

基金

收起

国家自然科学基金（31660652）;“强南”科技创新人才计划（2022CB001-04）;塔里木大学校长基金（TDZKCX202401）;塔里木大学研究生科研创新项目（TDGRI2024040）;

参考文献

收起

文献

收起

参考文献引证文献

排序方式：

[1]Schwabe J W R, Teichmann S A. Nuclear receptors:the evolution of diversity[J]. Sci STKE, 2004,(217):pe4.
[2]Jensen E V, Suzuki T, Kawashima T, et al. A two-step mechanism for the interaction of estradiol with rat uterus[J]. Proc Natl Acad Sci U.S.A, 1968, 59(2):632-638.
[3]Dorling A A, Todman M G, Korach K S, et al. Critical role for estrogen receptor alpha in negative feedback regulation of gonadotropin-releasing hormone mRNA expression in the female mouse[J]. Neuroendocrinology, 2003, 78(4):204-209.
[4]Dupont S, Krust A, Gansmuller A, et al. Effect of single and compound knockouts of estrogen receptors α(ERα)and β(ERβ)on mouse reproductive phenotypes[J]. Development,2000, 127(19):4277-4291.
[5]Deroo B J, Rodriguez K F, Couse J F, et al. Estrogen receptorβ is required for optimal cAMP production in mouse granulosa cells[J]. Mol Endocrinol, 2009, 23(7):955-965.
[6]Emmen J M A, Couse J F, Elmore S A, et al. In vitro growth and ovulation of follicles from ovaries of estrogen receptor(ER)α and ERβ null mice indicate a role for ERβ in follicular maturation[J]. Endocrinol, 2005, 146(6):2817-2826.
[7]Hegele-Hartung C, Siebel P, Peters O, et al. Impact of isotypeselective estrogen receptor agonists on ovarian function[J]. Proc Natl Acad Sci USA, 2004, 101(14):5129-5134.
[8]Vanderhyden B. Molecular basis of ovarian development and function[J]. Front Biosci, 2002, 7:d2-d6.
[9]Niu X, Tyasi T L, Qin N, et al. Sequence variations in estrogen receptor 1 and 2 genes and their association with egg production traits in Chinese Dagu chickens[J]. Vet Med Sci, 2017,79(5):927-934.
[10]Laliotis G P, Marantidis A, Avdi M. Association of BF, RBP4,and ESR2 genotypes with litter size in an autochthonous pig population[J]. Anim Biotechnol, 2017, 28(2):138-143.
[11]Wang K, Li Q, Niu Z, et al. Using Whole-Genome sequencing data reveals the population structure and selection signatures for reproduction traits in Duolang sheep[J]. Animals, 2025,15(23):3466.
[12]Lemon B, Tjian R. Orchestrated response:a symphony of transcription factors for gene controly[J]. Genes Dev, 2000, 14(20):2551-2569.
[13]国家畜禽遗传委员会.中国畜禽遗传资源志·羊志[M].北京:中国农业出版社, 2011:136.
[14]Chen H Y, Shen H, Jia B, et al. Differential gene expression in ovaries of Qira black sheep and Hetian sheep using RNA-Seq technique[J]. PLoS One, 2015, 10(3):0120170.
[15]於建国,董红,季跃光,等.和田羊品种资源现状与保护利用措施[J].草食家畜, 2011(2):7-9.
[16]Khristi V, Chakravarthi V P, Singh P, et al. ESR2 regulates granulosa cell genes essential for follicle maturation and ovulation[J]. Mol Cell Endocrinol, 2018, 474:214-226.
[17]Antonson P, Apolinário L M, Shamekh M M, et al. Generation of an all-exon ESR2 deleted mouse line:effects on fertility[J].Biochem Biophys Res Commun, 2020, 529(2):231-237.
[18]Xu S S, Gao L, Xie X L, et al. Genome-wide association analyses highlight the potential for different genetic mechanisms for litter size among sheep breeds[J]. Front Genet, 2018,9:118.
[19]Potenza C, Aleman L, Sengupta-Gopalan C. Targeting transgene expression in research, agricultural, and environmental applications:promoters used in plant transformation[J]. In Vitro Cell Dev Biol-Plant, 2004, 40:1-22.
[20]Hernandez-Garcia C M, Finer J J. Identification and validation of promoters and cis-acting regulatory elements[J]. Plant Sci,2014, 217:109-119.
[21]Kim Y, Geiger J H, Hahn S, et al. Crystal structure of a yeast TBP/TATA-box complex[J]. Nature, 1993, 365(6446):512-520.
[22]Zhao X, Li J, Zhao S, et al. Regulation of bta-miRNA29d-3p on lipid accumulation via GPAM in bovine mammary epithelial cells[J]. Agriculture, 2023, 13(2):501.
[23]Oh Y M, Mahar M, Ewan E E, et al. Epigenetic regulator UHRF1 inactivates REST and growth suppressor gene expression via DNA methylation to promote axon regeneration[J]. Proc Natl Acad Sci U S A, 2018, 115(52):E12417-E12426.
[24]Weidemüller P, Kholmatov M, Petsaluki E, et al. Transcription factors:bridge between cell signaling and gene regulation[J].Proteomics, 2021, 21(23-24):2000034.
[25]He H, Yang M, Li S, et al. Mechanisms and biotechnological applications of transcription factors[J]. Synth Syst Biotechnol,2023, 8(4):566-577.
[26]Hirano M, Wache-Hiraike O, Fu H, et al. The emerging role of FOXL2 in regulating the transcriptional activation function of estrogen receptor β:an insight into ovarian folliculogenesis[J].Reprod Sci, 2017, 24(1):133-141.
[27]Zhang J, Zhang M, Acampora D, et al. OTX2 restricts entry to the mouse germline[J]. Nature, 2018, 562(7728):595-599.
[28]Haberle V, Lenhard B. Promoter architectures and developmental gene regulation[C]. Semin Cell Dev Biol, 2016, 57:11-23.
[29]Wang J, Lang B, Zhu H, et al. Cloning and transcriptional activity analysis of the porcine cofilin 2 gene promoter[J].Gene, 2014, 547(2):280-287.
[30]Annicotte J S, Schoonjans K, Haby C, et al. An E-box in pGL3reporter vectors precludes their use for the study of sterol regulatory element-binding proteins[J]. Biotechniques, 2001,31(5):993-994, 996.

2026年第62卷第2期

PDF下载

引用本文

BibTeX

文章信息

doi: 10.19556/j.0258-7033.20250328-02

首发时间：2026-07-01
出版时间：2026-02-10

补充材料

相关文章

文章信息

作者

出版历史

基金

作者信息

参考文献

分享链接

https://castjournals.cast.org.cn/joweb/zgxmzz/CN/10.19556/j.0258-7033.20250328-02

分享至

全文二维码

扫描看全文

引用本文

BibTeX

本文的引用情况

2种不同金属材料的力学参数

科 Family	属数 Number of genus	种数 Number of species	占总种数比例 Percentage of total species (%)	属 Genus	种数 Number of species	占总种数比例 Percentage of total species (%)
鹅膏菌科Amanitaceae	2	11	5.26	鹅膏菌属 Amanita	10	4.78
小菇科 Mycenaceae	2	12	5.74	丝盖伞属 Inocybe	5	2.39
多孔菌科 Polyporaceae	8	14	6.70	蜡蘑属 Laccaria	5	2.39
红菇科 Russulaceae	3	23	11.00	小皮伞属 Marasmius	6	2.87
				小菇属 Mycena	11	5.26
				光柄菇属 Pluteus	5	2.39
				红菇属 Russula	17	8.13
				栓菌属 Trametes	5	2.39

关闭全屏

BibTeX
EndNote
RefWorks
TxT