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tenantId=1146029695717560320, journalId=1279045329999892481, language=CN, title=白藜芦醇对过氧化氢诱导小鼠睾丸间质细胞氧化损伤与凋亡的作用及分子机制, columnId=1282278076700660488, journalTitle=中国畜牧杂志, columnName=畜牧生物技术, runingTitle=null, highlight=null, articleAbstract=试验旨在研究白藜芦醇（RES）对过氧化氢（H 2 O 2 ）诱导小鼠睾丸间质细胞TM3氧化损伤和凋亡的作用及其分子机制。采用150μmol/L H 2 O 2 处理TM3细胞8 h诱导氧化损伤与凋亡，以5μmol/L RES干预24 h，采用比色法检测细胞培养液中乳酸脱氢酶（LDH）、细胞中谷胱甘肽过氧化物酶（GSH-Px）及丙二醛（MDA）；通过DCF-DA探针法检测活性氧（ROS）的产生；通过流式细胞术分析细胞凋亡，并利用ELISA检测孕酮含量；进一步通过real-time PCR检测基质金属蛋白酶（MMP）-2/9 mRNA的表达，并利用明胶酶谱和ELISA检测细胞上清中MMP-2/9的水平；最后通过Western blot检测SIRT1/AMPK通路部分自噬分子的表达。结果显示：在H 2 O 2 诱导的TM3细胞中，RES下调其LDH、MDA及ROS水平，显著提高GSH-Px活性，减少细胞凋亡，并促进睾酮的分泌；同时RES显著下调MMP-2/9 mRNA的表达及其酶原与活性形式；此外，RES通过激活AMPK的磷酸化及沉默信息调节因子1（SIRT1）表达，促进自噬标志物LC3-I向LC3-II的转化，且SIRT1和AMPK抑制剂均可逆转此效应。综合以上，RES通过SIRT1/AMPK通路调控自噬并抑制MMP-2/9活性，缓解H 2 O 2 诱导的TM3细胞氧化损伤与凋亡。, authors=蒙丽舟, 张晓春, 陈指龙, 谷婷, 袁安文, 杨青,, authorsList=蒙丽舟, 张晓春, 陈指龙, 谷婷, 袁安文, 杨青,, authorCompany=湖南农业大学动物医学院, correspAuthors=杨青, 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