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tenantId=1146029695717560320, journalId=1279045329999892481, language=CN, title=柯乐猪源Bacillus velezensis YY24的鉴定及产酶特性分析, columnId=1282278072095326533, journalTitle=中国畜牧杂志, columnName=动物健康, runingTitle=null, highlight=null, articleAbstract=为发掘柯乐猪肠道纤维素降解菌资源,本研究对其粪便源分离菌YY24进行鉴定及产酶特性分析。通过刚果红染色法检测菌株纤维素酶活性,结合形态观察与分子生物学对菌株进行菌种鉴定;采用单因素试验优化产酶条件;并开展全基因组测序与功能注释分析。刚果红染色结果显示,菌株在CMC-Na平板上形成明显水解圈,透明圈直径/菌落直径(D/d)为4.5。综合菌种鉴定结果,确定其为贝莱斯芽孢杆菌(Bacillus velezensis)。初步探究产酶条件发现:培养时间48 h、pH 7.0、接种龄3 d、接种量5%条件下酶活最高,达(17.33±0.27 )U/mL。进一步开展全基因组测序与注释分析,拼接获得3.95 Mb基因组草图,GC含量为46.45%。KEGG注释共识别1 896个功能基因,其中碳水化合物代谢通路基因最丰富;CAZy注释识别到61个碳水化合物活性酶基因,涵盖GH、AA等多个家族。综合注释结果发现候选基因eglS,其编码蛋白属于GH5家族成员内切葡聚糖酶,是一类稳定且亲水的分泌蛋白,功能域明确、柔性良好、结构简单。本研究结果为YY24的功能挖掘及在饲料微生态制剂领域的开发提供理论依据与候选菌株资源。, authors=王敏乐1 , 王娅丽1 , 吴诗卉1 , 周桂仙1 , 杨颖1,2 , authorsList=王敏乐, 王娅丽, 吴诗卉, 周桂仙, 杨颖, authorCompany=1.贵州大学动物科学学院; 2.贵州省动物生物制品工程技术研究中心, correspAuthors=杨颖, authorNote=王敏乐(2000-),男,陕西宝鸡人,硕士研究生,主要从事动物肠道益生菌研究,E-mail:gs.wangml23@gzu.edu.cn;, correspAuthorsNote=null, 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aliEndDate=null, collectionFlag=false, citedCount=null, citedUrl=null, previewStatus=0, delFlag=0, hasFullText=0, orderTime=1763654400000, fullTextJson=null, articleText=null, reference=[1]Bajaj P, Mahajan R. Cellulase and xylanase synergism in industrial biotechnology[J]. Appl Microbiol Biotechnol, 2019,103(21-22):8711-8724. [2]朱旗军,刘魏魏,杨培龙,等.我国秸秆转化技术及其在畜牧业上的应用[J].中国饲料, 2024(13):1-7. [3]Sutaoney P, Rai S N, Sinha S, et al. Current perspective in research and industrial applications of microbial cellulases[J].Int J Biol Macromol, 2024, 264(Pt 1):130639. [4]阳治康,殷运菊,徐伟伟,等.纤维素酶的酶学特性及在畜禽生产中的应用研究进展[J].中国畜牧杂志, 2024, 60(5):88-93. [5]朱波,赵青海,钟蕾,等.金针菇菌渣饲料中添加纤维素酶及甘露寡糖对草鱼生长、肠道结构及血清生理生化指标的影响[J].饲料工业, 2021, 42(4):41-47. [6]Raza A, Bashir S, Pothula R, et al. Expression and functional characterization in yeast of an endoglucanase from Bacillus sonorensis BD92 and its impact as feed additive in commercial broilers[J]. Int J Biol Macromol, 2021, 176:364-375. [7]黄志远.饲粮中添加纤维素酶对肉鸡的影响[J].中国畜牧业,2024(15):53-55. [8]王圣,宋德荣,侯健,等.贵州省赫章县柯乐猪产业现状及可持续性发展探索[J].养殖与饲料, 2024, 23(7):42-45. [9]杨莲,燕志宏,黄维江,等.纯种柯乐猪与巴×柯杂交猪肠道菌群结构的研究[J].动物营养学报, 2021, 33(3):1359-1371. [10]杨仕钰.不同养殖模式柯乐猪大肠微生物区系及其纤维降解菌筛选研究[D].贵阳:贵州大学, 2021. [11]赵婷婷,孙佳琪,王文迪,等.贝莱斯芽胞杆菌对动物的益生作用研究进展[J].中国畜牧杂志, 2023, 59(7):82-86. [12]吴诗丽,谢莹莹,段雪凝,等.产纤维素酶菌株的筛选及其酶学性质研究[J].食品工业科技, 2024:1-14. [13]林淑华,谭娟娟,杨贝莹,等.野猪肠道菌群多样性分析及其益生菌的分离筛选[J].微生物学通报, 2025, 52(2):757-770. [14]顾明慧,范瑞洋,王道晨,等.布氏田鼠盲肠中纤维素分解菌的分离鉴定及其产酶条件优化[J].扬州大学学报(农业与生命科学版), 2023, 44(4):90-97. [15]Abel-AzizHussein,吴家勇,姜海龙.猪盲肠中纤维素分解菌的分离鉴定[J].吉林畜牧兽医, 2019, 40(8):5-8. [16]姜立春,付丹阳,赵欣悦,等.麦洼牦牛粪便纤维素降解菌的筛选、鉴定及产酶条件优化[J].江苏农业科学, 2024,52(7):223-230. [17]田思齐,郭爱伟,和芳,等.大熊猫源纤维素分解菌的筛选及产酶发酵条件优化研究[J].西南林业大学学报(自然科学), 2023, 43(4):197-204. [18]李村院,李晓悦,刘夏,等.马盲肠纤维素分解菌的分离鉴定与酶活分析[J].家畜生态学报, 2022, 43(5):28-34. [19]Kenfaoui J, Dutilloy E, Benchlih S, et al. Bacillus velezensis:a versatile ally in the battle against phytopathogens-insights and prospects[J]. Appl Microbiol Biotechnol, 2024, 108(1):439. [20]张津浩,邓辉,张清壮,等.贝莱斯芽胞杆菌XY40-1对百合球茎生长、品质及镉含量的调控作用[J].生物技术通报,2025, 41(7):271-291. [21]黄悦姝,邓超,张馨月,等.贝莱斯芽孢杆菌D103产纤维素酶优化及途径分析[J].生物技术, 2023, 33(5):632-640. [22]Lu W, Zha B, Lyu J, et al. Whole-genome sequencing and genomic analysis of four Akkermansia strains newly isolated from human feces[J]. Front Microbiol, 2024, 15:1500886. [23]王春玲,黄钰婷,刘星,等.一株贝莱斯芽孢杆菌鉴定、全基因组学分析及其抗病促生特性[J].微生物学报, 2025,65(2):745-757. [24]Suchova K, Feher C, Ravn J L, et al. Cellulose-and xylandegrading yeasts:Enzymes, applications and biotechnological potential[J]. Biotechnol Adv, 2022, 59:107981. [25]马建飞,徐均钊,胡宗福,等.蒙古牛瘤胃微生物的宏基因组学分析[J].中国畜牧杂志, 2025, 61(3):260-268. [26]Zheng F, Vermaas J V, Zheng J, et al. Activity and thermostability of GH5 endoglucanase chimeras from mesophilic and thermophilic parents[J]. Appl Environ Microbiol, 2019, 85(5):e02079-18. [27]杨俊钊,张新蕊,赵国柱,等.新型GH5家族多结构域纤维素酶的结构与功能研究[J].生物技术通报, 2023, 39(4):71-80. [28]Zhang S, Xu Z, Wang T, et al. Endoglucanase improve the growth of homofermentative Lactobacillus spp. in ensilages[J].J Biotechnol, 2019, 295:55-62. [29]圣弟青,钮成拓,闫亚楠,等.低温β-1,4-内切葡聚糖酶的基因挖掘、异源表达及其酶学性质表征[J].食品与发酵工业,2024, 50(4):17-24. [30]沈晓静,赵健,王海娟,等. 1株内切葡聚糖酶基因Cel042的克隆与表达[J].安徽农业科学, 2025:1-5.)
中国畜牧杂志
|动物健康
2026
, 62
(2) :
333
-340
柯乐猪源Bacillus velezensis YY24的鉴定及产酶特性分析
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王敏乐, 王娅丽, 吴诗卉, 周桂仙, 杨颖
作者信息
作者简介:
王敏乐(2000-),男,陕西宝鸡人,硕士研究生,主要从事动物肠道益生菌研究,E-mail:gs.wangml23@gzu.edu.cn;
Affiliations
出版时间: 2025-11-21
doi: 10.19556/j.0258-7033.20250526-03
文章导航
为发掘柯乐猪肠道纤维素降解菌资源,本研究对其粪便源分离菌YY24进行鉴定及产酶特性分析。通过刚果红染色法检测菌株纤维素酶活性,结合形态观察与分子生物学对菌株进行菌种鉴定;采用单因素试验优化产酶条件;并开展全基因组测序与功能注释分析。刚果红染色结果显示,菌株在CMC-Na平板上形成明显水解圈,透明圈直径/菌落直径(D/d)为4.5。综合菌种鉴定结果,确定其为贝莱斯芽孢杆菌(Bacillus velezensis)。初步探究产酶条件发现:培养时间48 h、pH 7.0、接种龄3 d、接种量5%条件下酶活最高,达(17.33±0.27 )U/mL。进一步开展全基因组测序与注释分析,拼接获得3.95 Mb基因组草图,GC含量为46.45%。KEGG注释共识别1 896个功能基因,其中碳水化合物代谢通路基因最丰富;CAZy注释识别到61个碳水化合物活性酶基因,涵盖GH、AA等多个家族。综合注释结果发现候选基因eglS,其编码蛋白属于GH5家族成员内切葡聚糖酶,是一类稳定且亲水的分泌蛋白,功能域明确、柔性良好、结构简单。本研究结果为YY24的功能挖掘及在饲料微生态制剂领域的开发提供理论依据与候选菌株资源。
柯乐猪
/
纤维素降解菌
/
贝莱斯芽孢杆菌
/
产酶优化
/
内切葡聚糖酶
王敏乐, 王娅丽, 吴诗卉, 周桂仙, 杨颖.
柯乐猪源Bacillus velezensis YY24的鉴定及产酶特性分析.
中国畜牧杂志,
2026
, 62
(2)
: 333
-340
.
DOI: 10.19556/j.0258-7033.20250526-03
参考文献
引证文献
[1]Bajaj P, Mahajan R. Cellulase and xylanase synergism in industrial biotechnology[J]. Appl Microbiol Biotechnol, 2019,103(21-22):8711-8724. [2]朱旗军,刘魏魏,杨培龙,等.我国秸秆转化技术及其在畜牧业上的应用[J].中国饲料, 2024(13):1-7. [3]Sutaoney P, Rai S N, Sinha S, et al. Current perspective in research and industrial applications of microbial cellulases[J].Int J Biol Macromol, 2024, 264(Pt 1):130639. [4]阳治康,殷运菊,徐伟伟,等.纤维素酶的酶学特性及在畜禽生产中的应用研究进展[J].中国畜牧杂志, 2024, 60(5):88-93. [5]朱波,赵青海,钟蕾,等.金针菇菌渣饲料中添加纤维素酶及甘露寡糖对草鱼生长、肠道结构及血清生理生化指标的影响[J].饲料工业, 2021, 42(4):41-47. [6]Raza A, Bashir S, Pothula R, et al. Expression and functional characterization in yeast of an endoglucanase from Bacillus sonorensis BD92 and its impact as feed additive in commercial broilers[J]. Int J Biol Macromol, 2021, 176:364-375. [7]黄志远.饲粮中添加纤维素酶对肉鸡的影响[J].中国畜牧业,2024(15):53-55. [8]王圣,宋德荣,侯健,等.贵州省赫章县柯乐猪产业现状及可持续性发展探索[J].养殖与饲料, 2024, 23(7):42-45. [9]杨莲,燕志宏,黄维江,等.纯种柯乐猪与巴×柯杂交猪肠道菌群结构的研究[J].动物营养学报, 2021, 33(3):1359-1371. [10]杨仕钰.不同养殖模式柯乐猪大肠微生物区系及其纤维降解菌筛选研究[D].贵阳:贵州大学, 2021. [11]赵婷婷,孙佳琪,王文迪,等.贝莱斯芽胞杆菌对动物的益生作用研究进展[J].中国畜牧杂志, 2023, 59(7):82-86. [12]吴诗丽,谢莹莹,段雪凝,等.产纤维素酶菌株的筛选及其酶学性质研究[J].食品工业科技, 2024:1-14. [13]林淑华,谭娟娟,杨贝莹,等.野猪肠道菌群多样性分析及其益生菌的分离筛选[J].微生物学通报, 2025, 52(2):757-770. [14]顾明慧,范瑞洋,王道晨,等.布氏田鼠盲肠中纤维素分解菌的分离鉴定及其产酶条件优化[J].扬州大学学报(农业与生命科学版), 2023, 44(4):90-97. [15]Abel-AzizHussein,吴家勇,姜海龙.猪盲肠中纤维素分解菌的分离鉴定[J].吉林畜牧兽医, 2019, 40(8):5-8. [16]姜立春,付丹阳,赵欣悦,等.麦洼牦牛粪便纤维素降解菌的筛选、鉴定及产酶条件优化[J].江苏农业科学, 2024,52(7):223-230. [17]田思齐,郭爱伟,和芳,等.大熊猫源纤维素分解菌的筛选及产酶发酵条件优化研究[J].西南林业大学学报(自然科学), 2023, 43(4):197-204. [18]李村院,李晓悦,刘夏,等.马盲肠纤维素分解菌的分离鉴定与酶活分析[J].家畜生态学报, 2022, 43(5):28-34. [19]Kenfaoui J, Dutilloy E, Benchlih S, et al. Bacillus velezensis:a versatile ally in the battle against phytopathogens-insights and prospects[J]. Appl Microbiol Biotechnol, 2024, 108(1):439. [20]张津浩,邓辉,张清壮,等.贝莱斯芽胞杆菌XY40-1对百合球茎生长、品质及镉含量的调控作用[J].生物技术通报,2025, 41(7):271-291. [21]黄悦姝,邓超,张馨月,等.贝莱斯芽孢杆菌D103产纤维素酶优化及途径分析[J].生物技术, 2023, 33(5):632-640. [22]Lu W, Zha B, Lyu J, et al. Whole-genome sequencing and genomic analysis of four Akkermansia strains newly isolated from human feces[J]. Front Microbiol, 2024, 15:1500886. [23]王春玲,黄钰婷,刘星,等.一株贝莱斯芽孢杆菌鉴定、全基因组学分析及其抗病促生特性[J].微生物学报, 2025,65(2):745-757. [24]Suchova K, Feher C, Ravn J L, et al. Cellulose-and xylandegrading yeasts:Enzymes, applications and biotechnological potential[J]. Biotechnol Adv, 2022, 59:107981. [25]马建飞,徐均钊,胡宗福,等.蒙古牛瘤胃微生物的宏基因组学分析[J].中国畜牧杂志, 2025, 61(3):260-268. [26]Zheng F, Vermaas J V, Zheng J, et al. Activity and thermostability of GH5 endoglucanase chimeras from mesophilic and thermophilic parents[J]. Appl Environ Microbiol, 2019, 85(5):e02079-18. [27]杨俊钊,张新蕊,赵国柱,等.新型GH5家族多结构域纤维素酶的结构与功能研究[J].生物技术通报, 2023, 39(4):71-80. [28]Zhang S, Xu Z, Wang T, et al. Endoglucanase improve the growth of homofermentative Lactobacillus spp. in ensilages[J].J Biotechnol, 2019, 295:55-62. [29]圣弟青,钮成拓,闫亚楠,等.低温β-1,4-内切葡聚糖酶的基因挖掘、异源表达及其酶学性质表征[J].食品与发酵工业,2024, 50(4):17-24. [30]沈晓静,赵健,王海娟,等. 1株内切葡聚糖酶基因Cel042的克隆与表达[J].安徽农业科学, 2025:1-5.
2026年第62卷第2期
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doi: 10.19556/j.0258-7033.20250526-03
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2种不同金属材料的力学参数
科 Family 属数 Number of genus 种数 Number of species 占总种数比例 Percentage of total species (%) 属 Genus 种数 Number of species 占总种数比例 Percentage of total species (%) 鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78 小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39 多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39 红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87 小菇属 Mycena 11 5.26 光柄菇属 Pluteus 5 2.39 红菇属 Russula 17 8.13 栓菌属 Trametes 5 2.39
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