Article(id=1279144335614652722, tenantId=1146029695717560320, journalId=1279045329999892481, issueId=1279144148057960780, articleNumber=PA20260323_iPc39jyx, orderNo=null, doi=10.19556/j.0258-7033.20250917-05, pmid=null, cstr=null, oa=null, hot=0, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=null, receivedDateStr=null, revisedDate=null, revisedDateStr=null, acceptedDate=null, acceptedDateStr=null, onlineDate=1782901374964, onlineDateStr=2026-07-01, pubDate=1767456000000, pubDateStr=2026-01-04, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1782901374963, onlineIssueDateStr=2026-07-01, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1782901374963, creator=admin, updateTime=1782901374963, updator=admin, issue=Issue{id=1279144148057960780, tenantId=1146029695717560320, journalId=1279045329999892481, year='2026', volume='62', issue='2', pageStart='1', pageEnd='388', issueExtLink='null', onlineDate='null', pubDate='1770652800000', pubDateStr='2026-02-10', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=1, specialIssue=null, createTime=1782901330245, creator='ztmeta', updateTime=1783650204703, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1282285155289108897, tenantId=1146029695717560320, journalId=1279045329999892481, issueId=1279144148057960780, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1282285155289108898, tenantId=1146029695717560320, journalId=1279045329999892481, issueId=1279144148057960780, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=211, endPage=217, ext={CN=ArticleExt(id=1284209640045003689, articleId=1279144335614652722, tenantId=1146029695717560320, journalId=1279045329999892481, language=CN, title=PML基因SNP与柯乐猪繁殖性状的关联分析, columnId=1282278062347764011, journalTitle=中国畜牧杂志, columnName=遗传育种, runingTitle=null, highlight=null, articleAbstract=试验选取240头健康的经产柯乐猪，利用PCR扩增和Sanger测序法筛选PML基因的SNP；使用SPSS 27.0软件对SNP位点与柯乐猪的繁殖性状进行关联性分析；使用在线软件RNAfold、SHEsis、STRING等对SNP位点进行生物信息学分析。结果发现PML基因第2外显子的g.59407427 A>C、第7内含子的g.59444512 G>A以及第8内含子的g.59444894 A>G。连锁不平衡分析显示g.59407427 A>C和g.59444894A>G之间存在强连锁关系（r 2 >0.88）。单倍型分析显示共存在4种单倍型和8种双倍型，群体优势双倍型为H1H1（AAAAGG）。进一步与繁殖性状关联分析显示，3个SNP对总产仔数、产活仔数、初生窝重、断奶仔猪数和断奶窝重均有显著影响，双倍型H2H3（AAAGAG）个体的产活仔数、初生窝重、断奶窝重和断奶仔猪数均高于其他双倍型，为群体有利双倍型；g.59407427 A>C没有导致氨基酸序列的改变，但mRNA二级结构发生了变化，影响了结构稳定性。在PML蛋白互作蛋白网络中主要互作蛋白DAXX、RARA、RUNX1、SUMO1、SPI1与免疫功能、发育调控和抗病性密切相关，为后续研究基因功能和调控机制提供了方向。本研究发现了3个对柯乐猪繁殖性状有显著影响的SNP，可作为柯乐猪繁殖性状选育的遗传标记进一步研究。, authors=付红梅¹, 张游宇², 杨齐心², 杨红文², 顾丽菊³, 肖礼华⁴, 张依裕¹, authorsList=付红梅, 张游宇, 杨齐心, 杨红文, 顾丽菊, 肖礼华, 张依裕, authorCompany=1.贵州大学动物科学学院,高原山地动物遗传育种与繁殖教育部重点实验室,贵州省动物遗传育种与繁殖重点实验室,贵州大学香猪研究所;
2.贵州省畜禽遗传资源管理站;
3.贵州优农谷生态产业有限公司;
4.遵义市畜牧渔业站, correspAuthors=肖礼华, authorNote=付红梅（2000-），女，贵州凤冈县人，硕士研究生，研究方向：动物遗传育种与繁殖，E-mail:15185311729@163.com;, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=vOPwE4So3A/+Imr8q5xLYQ==, pdfFileSize=2450686, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=贵州省生猪产业科技创新项目（黔农计财[2025]9号、黔农计财[2024]33号）;贵州省生猪产业技术体系健康养殖功能实验室和遵义综合试验站建设项目（GZSZCYJSTX-03、GZSZCYJSTX-07）;国家重点研发计划子课题（2022YFD1100308-01）;贵州省科技计划项目（黔科合平台人才[2021]5630号）;贵州省科技支撑计划（黔科合支撑[2021]一般147）;)}, authors=null, keywords=[Keyword(id=1284217199833367406, tenantId=1146029695717560320, journalId=1279045329999892481, articleId=1279144335614652722, language=CN, orderNo=1, keyword=PML基因), Keyword(id=1284217199896281967, tenantId=1146029695717560320, 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