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中国畜牧杂志
|遗传育种
2026
, 62
(2) :
197
-205
罗盘山猪脂肪间充质干细胞体外培养及生物学特性研究
全屏
王仲发1,2 , 李旻娟1,2 , 刘晏辰1 , 何玉楠1,3 , 刘文忠2 , 关伟军1
作者信息
1.中国农业科学院北京畜牧兽医研究所; 2.山西农业大学动物科学学院; 3.西北农林科技大学动物科技学院
通讯作者:
刘文忠
作者简介:
王仲发(1997-),男,甘肃定西人,硕士研究生,主要从事干细胞研究,E-mail:2812539972@qq.com; 李旻娟(2000-),女,山东临沂人,硕士研究生,主要从事干细胞研究,E-mail:2171264303@qq.com;
Affiliations
出版时间: 2025-12-29
doi: 10.19556/j.0258-7033.20250217-06
文章导航
试验旨在通过体外分离培养罗盘山猪脂肪间充质干细胞(Adipose-derived Mesenchymal Stem Cells,ADSCs),探究其生物学特性和诱导分化潜能。采用I型胶原酶消化法体外获得罗盘山猪ADSCs,通过RTPCR和免疫荧光技术鉴定ADSCs表面标志物的表达;通过生长曲线绘制、划痕试验和克隆团形成试验,探究其体外增殖趋势和生长发育规律;通过成骨细胞、成脂细胞和成软骨细胞诱导,探究其体外分化能力;通过染色体核型分析,探究其在体外环境中遗传物质的稳定性。RT-PCR和免疫荧光结果表明,ADSCs能够表达CD29、CD44、CD73、CD90,不表达与造血干细胞相关的CD34;通过胰蛋白酶消化法分离出的ADSCs,随着细胞代次的增加,细胞活力逐渐降低;细胞划痕和克隆结果表明,随着代次的升高,细胞体外增殖能力不断下降,生长曲线呈“S”型;体外诱导罗盘山猪ADSCs,能够诱导出成骨细胞、成脂肪细胞和成软骨细胞;染色体核型分析结果表明,在体外增殖过程中ADSCs能够保持稳定的染色体形态,染色体基因组未发生改变(2n=38)。本研究在体外成功分离出罗盘山猪ADSCs,且ADSCs具有较强的体外增殖和分化潜能,研究结果可为罗盘山猪种质资源的保护和保存提供方法、为组织工程学研究提供参考依据。
罗盘山猪
/
脂肪
/
间充质干细胞
/
生物学特性
王仲发, 李旻娟, 刘晏辰, 何玉楠, 刘文忠, 关伟军.
罗盘山猪脂肪间充质干细胞体外培养及生物学特性研究.
中国畜牧杂志,
2026
, 62
(2)
: 197
-205
.
DOI: 10.19556/j.0258-7033.20250217-06
参考文献
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2026年第62卷第2期
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doi: 10.19556/j.0258-7033.20250217-06
首发时间:2026-07-01
出版时间:2025-12-29
https://castjournals.cast.org.cn/joweb/zgxmzz/CN/10.19556/j.0258-7033.20250217-06
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2种不同金属材料的力学参数
科 Family 属数 Number of genus 种数 Number of species 占总种数比例 Percentage of total species (%) 属 Genus 种数 Number of species 占总种数比例 Percentage of total species (%) 鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78 小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39 多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39 红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87 小菇属 Mycena 11 5.26 光柄菇属 Pluteus 5 2.39 红菇属 Russula 17 8.13 栓菌属 Trametes 5 2.39
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