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tenantId=1146029695717560320, journalId=1279045329999892481, language=CN, title=不同海拔牦牛骨骼肌HSP70相关基因的表达分析, columnId=1282278062347764011, journalTitle=中国畜牧杂志, columnName=遗传育种, runingTitle=null, highlight=null, articleAbstract=为了探究不同海拔对牦牛（Bos grunniens）骨骼肌中HSP70相关基因的表达特点及其低氧适应机制，试验选取青海省化隆县（海拔1 900 m，简称低海拔组）、海晏县（3 200 m，简称中海拔组）和班玛县（4 500 m，简称高海拔组）的健康雄性成年牦牛各5头，屠宰后取骨骼肌（股四头肌）组织置于4%多聚甲醛溶液固定，另取部分组织在液氮中保存。以低海拔牦牛作为对照，用实时荧光定量PCR和蛋白免疫印迹技术检测不同海拔牦牛骨骼肌HSP70相关基因的mRNA和蛋白的表达变化。结果表明，低氧会诱导缺氧诱导因子1(Hypoxia-Inducible Factor-1,HIF1)的产生，并且长期的缺氧环境会造成骨骼肌中线粒体的损伤，同时诱导一些热应激因子发挥作用，共同维持细胞代谢和能量代谢的守恒，从而增强机体对低氧的适应。本研究为青海地区高原动物的低氧适应机制以及高原病的治疗提供了理论依据和新的思路。, authors=付一依, 权君莲, 张勤文,, authorsList=付一依, 权君莲, 张勤文,, authorCompany=青海大学农牧学院, correspAuthors=张勤文, authorNote=付一依（2001-），女，硕士研究生，研究方向为高原家畜形态学结构特点与高原环境的关系，E-mail:1179961999@qq.com;, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=Vj89FuyfuEQPkBruGuS8WA==, 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