Article(id=1279144335614652722, tenantId=1146029695717560320, journalId=1279045329999892481, issueId=1279144148057960780, articleNumber=PA20260323_iPc39jyx, orderNo=null, doi=10.19556/j.0258-7033.20250917-05, pmid=null, cstr=null, oa=null, hot=0, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=null, receivedDateStr=null, revisedDate=null, revisedDateStr=null, acceptedDate=null, acceptedDateStr=null, onlineDate=1782901374964, onlineDateStr=2026-07-01, pubDate=1767456000000, pubDateStr=2026-01-04, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1782901374963, onlineIssueDateStr=2026-07-01, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1782901374963, creator=admin, updateTime=1782901374963, updator=admin, issue=Issue{id=1279144148057960780, tenantId=1146029695717560320, journalId=1279045329999892481, year='2026', volume='62', issue='2', pageStart='1', pageEnd='388', issueExtLink='null', onlineDate='null', pubDate='1770652800000', pubDateStr='2026-02-10', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=1, specialIssue=null, createTime=1782901330245, creator='ztmeta', updateTime=1783650204703, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1282285155289108897, tenantId=1146029695717560320, journalId=1279045329999892481, issueId=1279144148057960780, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1282285155289108898, tenantId=1146029695717560320, journalId=1279045329999892481, issueId=1279144148057960780, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=211, endPage=217, ext={CN=ArticleExt(id=1284209640045003689, articleId=1279144335614652722, tenantId=1146029695717560320, journalId=1279045329999892481, language=CN, title=PML基因SNP与柯乐猪繁殖性状的关联分析, columnId=1282278062347764011, journalTitle=中国畜牧杂志, columnName=遗传育种, runingTitle=null, highlight=null, articleAbstract=试验选取240头健康的经产柯乐猪,利用PCR扩增和Sanger测序法筛选PML基因的SNP;使用SPSS 27.0软件对SNP位点与柯乐猪的繁殖性状进行关联性分析;使用在线软件RNAfold、SHEsis、STRING等对SNP位点进行生物信息学分析。结果发现PML基因第2外显子的g.59407427 A>C、第7内含子的g.59444512 G>A以及第8内含子的g.59444894 A>G。连锁不平衡分析显示g.59407427 A>C和g.59444894A>G之间存在强连锁关系(r 2 >0.88)。单倍型分析显示共存在4种单倍型和8种双倍型,群体优势双倍型为H1H1(AAAAGG)。进一步与繁殖性状关联分析显示,3个SNP对总产仔数、产活仔数、初生窝重、断奶仔猪数和断奶窝重均有显著影响,双倍型H2H3(AAAGAG)个体的产活仔数、初生窝重、断奶窝重和断奶仔猪数均高于其他双倍型,为群体有利双倍型;g.59407427 A>C没有导致氨基酸序列的改变,但mRNA二级结构发生了变化,影响了结构稳定性。在PML蛋白互作蛋白网络中主要互作蛋白DAXX、RARA、RUNX1、SUMO1、SPI1与免疫功能、发育调控和抗病性密切相关,为后续研究基因功能和调控机制提供了方向。本研究发现了3个对柯乐猪繁殖性状有显著影响的SNP,可作为柯乐猪繁殖性状选育的遗传标记进一步研究。, authors=付红梅1 , 张游宇2 , 杨齐心2 , 杨红文2 , 顾丽菊3 , 肖礼华4 , 张依裕1 , authorsList=付红梅, 张游宇, 杨齐心, 杨红文, 顾丽菊, 肖礼华, 张依裕, authorCompany=1.贵州大学动物科学学院,高原山地动物遗传育种与繁殖教育部重点实验室,贵州省动物遗传育种与繁殖重点实验室,贵州大学香猪研究所; 2.贵州省畜禽遗传资源管理站; 3.贵州优农谷生态产业有限公司; 4.遵义市畜牧渔业站, correspAuthors=肖礼华, authorNote=付红梅(2000-),女,贵州凤冈县人,硕士研究生,研究方向:动物遗传育种与繁殖,E-mail:15185311729@163.com;, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=vOPwE4So3A/+Imr8q5xLYQ==, pdfFileSize=2450686, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=贵州省生猪产业科技创新项目(黔农计财[2025]9号、黔农计财[2024]33号);贵州省生猪产业技术体系健康养殖功能实验室和遵义综合试验站建设项目(GZSZCYJSTX-03、GZSZCYJSTX-07);国家重点研发计划子课题(2022YFD1100308-01);贵州省科技计划项目(黔科合平台人才[2021]5630号);贵州省科技支撑计划(黔科合支撑[2021]一般147);)}, authors=[Author(id=1288182988907188447, tenantId=1146029695717560320, journalId=null, articleId=1279144335614652722, orderNo=null, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=null, email=null, emailSecond=null, emailThird=null, correspondingAuthor=null, authorType=null, ext={CN=AuthorExt(id=null, tenantId=null, journalId=1279045329999892481, articleId=1279144335614652722, authorId=1288182988907188447, language=CN, stringName=付红梅, 张游宇, 杨齐心, 杨红文, 顾丽菊, 肖礼华, 张依裕, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=null, address=null, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=null)], keywords=[Keyword(id=1284217199833367406, tenantId=1146029695717560320, journalId=1279045329999892481, articleId=1279144335614652722, language=CN, orderNo=1, keyword=PML基因), Keyword(id=1284217199896281967, tenantId=1146029695717560320, journalId=1279045329999892481, articleId=1279144335614652722, language=CN, orderNo=2, keyword=SNP), Keyword(id=1284217199959196528, tenantId=1146029695717560320, journalId=1279045329999892481, articleId=1279144335614652722, language=CN, orderNo=3, keyword=柯乐猪), Keyword(id=1284217200013722481, tenantId=1146029695717560320, journalId=1279045329999892481, articleId=1279144335614652722, language=CN, orderNo=4, keyword=繁殖性状)], refs=null, funds=null, companyList=null, figs=null, attaches=null, journal=Journal(id=1279045211531776000, delFlag=0, nameCn=中国畜牧杂志, nameEn=Chinese Journal of Animal Science, nameHistory1=null, nameHistory2=null, issn=0258-7033, eissn=null, cn=11-2083/S, coden=null, periodic=0, language=CN, oaType=null, ccby=null, superviseOffice=null, ownerOffice=null, pubOffice=null, editorOffice=null, officeType=null, aims=null, clcCode=null, officeProv=null, officeCity=null, officeAddr=null, officeZip=null, officeEmail=null, officePhone=null, editDirector=null, officeDirector=null, officeDirectorPhone=null, officeStaffNum=null, officeEmpNum=null, coverPicUrl=/LwzP9ODDP1104PfuP3+5A==, journalPrice=null, startedYear=null, abbrevIsoEn=Chinese Journal of Animal Science, journalRemark=null, publicationField=null, createdTime=1782877741943, updatedTime=1784022333352, createdBy=18614031015, updatedBy=13041195026, firstLetterCn=Z, firstLetterEn=Z, subjectCode=Life Sciences, subjectName=null, subjectCodeEn=Life Sciences, subjectNameEn=null, picCn=/LwzP9ODDP1104PfuP3+5A==, picEn=se+5xDE//YSxHqLJ0K7Jaw==, jcr=null, cjcr=null, exts=[JournalExt(id=1283845976133713988, language=CN, name=中国畜牧杂志, nameHistory1=null, nameHistory2=null, managedBy=, sponsoredBy=, publishedBy=, editorOffice=, officeProv=null, officeCity=null, officeAddr=, officeZip=, editDirector=, officeDirector=null, officePhone=null, coverPicUrl=null, journalRemark=, submitArticleUrl=null, websiteUrl=, createdTime=1784022333415, updatedTime=1784022333415, createdBy=13041195026, updatedBy=13041195026, submissionGuidelinesUrl=, submissionAuthorUrl=https://zgxm.cbpt.cnki.net/EditorC3N/index.aspx?t=1, submissionEditorUrl=https://zgxm.cbpt.cnki.net/EditorC3N/index.aspx?t=3, submissionReviewUrl=https://zgxm.cbpt.cnki.net/EditorC3N/index.aspx?t=2, submissionCeEditorUrl=, submissionAeEditorUrl=, option={"copyright":""}), JournalExt(id=1283845976184045637, language=EN, name=Chinese Journal of Animal Science, nameHistory1=null, nameHistory2=null, managedBy=, sponsoredBy=, publishedBy=, editorOffice=, officeProv=null, officeCity=null, officeAddr=, officeZip=, editDirector=, officeDirector=null, officePhone=null, coverPicUrl=null, journalRemark=, submitArticleUrl=null, websiteUrl=, createdTime=1784022333427, updatedTime=1784022333427, createdBy=13041195026, updatedBy=13041195026, submissionGuidelinesUrl=, submissionAuthorUrl=https://zgxm.cbpt.cnki.net/EditorC3N/index.aspx?t=1, submissionEditorUrl=https://zgxm.cbpt.cnki.net/EditorC3N/index.aspx?t=3, submissionReviewUrl=https://zgxm.cbpt.cnki.net/EditorC3N/index.aspx?t=2, submissionCeEditorUrl=, submissionAeEditorUrl=, option={"copyright":""})], databaseList=null, tenantJournalId=1279045329999892481, websiteList=[Website(id=1279045564750869087, webName=null, webTitle=null, webDomain=null, webCopyrigh=null, webIpcNo=null, seoTitle=null, seoKeywords=null, seoDescription=null, tenantJournalId=null, journalId=1279045329999892481, journalNameCn=null, journalNameEn=null, grayFlag=null, tenantId=1146029695717560320, platformId=null, journalGroupId=null, journalGroupNameCn=null, journalGroupNameEn=null, type=1, domain=https://castjournals.cast.org.cn/joweb/zgxmzz/CN, language=CN, createTime=1782877826154, createBy=18614031015, updateTime=1782877970220, updateBy=18614031015, name=中国畜牧杂志-中文, tplId=1146099689490845704, title=中国畜牧杂志, delFlag=0, indexPage=/home, props=[WebsiteProps(id=1279046195876184733, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1279045564750869087, code=articleTextType, value=kx, createTime=1782877976626, updateTime=1782877976626, creator=18614031015, updator=18614031015), WebsiteProps(id=1279046195855213210, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1279045564750869087, code=banner, value=null, createTime=1782877976621, updateTime=1782877976621, creator=18614031015, updator=18614031015), WebsiteProps(id=1279046195892961952, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1279045564750869087, code=grayFlag, value=0, createTime=1782877976630, updateTime=1782877976630, creator=18614031015, updator=18614031015), WebsiteProps(id=1279046195842630297, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1279045564750869087, code=logo, value=https://castjournals.cast.org.cn/joweb/zgxmzz/CN/file/pic?fileId=HfoX2B/UbkzyU4RMsQSFQQ==, createTime=1782877976618, updateTime=1782877976618, creator=18614031015, updator=18614031015), WebsiteProps(id=1279046195905544866, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1279045564750869087, code=minRunFlag, value=0, createTime=1782877976633, updateTime=1782877976633, creator=18614031015, updator=18614031015), WebsiteProps(id=1279046195867796124, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1279045564750869087, code=picServerUrl, value=https://castjournals.cast.org.cn/joweb/zgxmzz/CN/file/pic, createTime=1782877976624, updateTime=1782877976624, creator=18614031015, updator=18614031015), WebsiteProps(id=1279046195901350561, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1279045564750869087, code=silenceFlag, value=0, createTime=1782877976632, updateTime=1782877976632, creator=18614031015, updator=18614031015), WebsiteProps(id=1279046195863601819, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1279045564750869087, code=staticResourcePath, value=https://castjournals.cast.org.cn/joweb/cast_kjdb_cn_619/, createTime=1782877976623, updateTime=1782877976623, creator=18614031015, updator=18614031015), WebsiteProps(id=1279046195880379038, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1279045564750869087, code=themeColor, value=null, createTime=1782877976627, updateTime=1782877976627, creator=18614031015, updator=18614031015), WebsiteProps(id=1279046195888767647, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1279045564750869087, code=themeStyle, value=null, createTime=1782877976629, updateTime=1782877976629, creator=18614031015, updator=18614031015)]), Website(id=1279045564864115298, webName=null, webTitle=null, webDomain=null, webCopyrigh=null, webIpcNo=null, seoTitle=null, seoKeywords=null, seoDescription=null, tenantJournalId=null, journalId=1279045329999892481, journalNameCn=null, journalNameEn=null, grayFlag=null, tenantId=1146029695717560320, platformId=null, journalGroupId=null, journalGroupNameCn=null, journalGroupNameEn=null, type=1, domain=https://castjournals.cast.org.cn/joweb/zgxmzz/EN, language=EN, createTime=1782877826181, createBy=18614031015, updateTime=1782877964219, updateBy=18614031015, name=中国畜牧杂志-英文, tplId=1146101810881728533, title=Chinese Journal of Animal Science, delFlag=0, indexPage=/home, props=[WebsiteProps(id=1279046222778450599, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1279045564864115298, code=articleTextType, value=kx, createTime=1782877983040, updateTime=1782877983040, creator=18614031015, updator=18614031015), WebsiteProps(id=1279046222749090468, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1279045564864115298, code=banner, value=null, createTime=1782877983033, updateTime=1782877983033, creator=18614031015, updator=18614031015), WebsiteProps(id=1279046222816199338, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1279045564864115298, code=grayFlag, value=0, createTime=1782877983049, updateTime=1782877983049, creator=18614031015, updator=18614031015), WebsiteProps(id=1279046222740701859, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1279045564864115298, code=logo, value=https://castjournals.cast.org.cn/joweb/zgxmzz/EN/file/pic?fileId=HfoX2B/UbkzyU4RMsQSFQQ==, createTime=1782877983031, updateTime=1782877983031, creator=18614031015, updator=18614031015), WebsiteProps(id=1279046222832976556, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1279045564864115298, code=minRunFlag, value=0, createTime=1782877983053, updateTime=1782877983053, creator=18614031015, updator=18614031015), WebsiteProps(id=1279046222765867686, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1279045564864115298, code=picServerUrl, value=https://castjournals.cast.org.cn/joweb/zgxmzz/EN/file/pic, createTime=1782877983037, updateTime=1782877983037, creator=18614031015, updator=18614031015), WebsiteProps(id=1279046222824587947, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1279045564864115298, code=silenceFlag, value=0, createTime=1782877983051, updateTime=1782877983051, creator=18614031015, updator=18614031015), WebsiteProps(id=1279046222757479077, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1279045564864115298, code=staticResourcePath, value=https://castjournals.cast.org.cn/joweb/cast_kjdb_en_623/, createTime=1782877983035, updateTime=1782877983035, creator=18614031015, updator=18614031015), WebsiteProps(id=1279046222791033512, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1279045564864115298, code=themeColor, value=null, createTime=1782877983043, updateTime=1782877983043, creator=18614031015, updator=18614031015), WebsiteProps(id=1279046222803616425, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1279045564864115298, code=themeStyle, value=null, createTime=1782877983046, updateTime=1782877983046, creator=18614031015, updator=18614031015)])], journalTitle=中国畜牧杂志, weixinUrl=null, journalUrl=https://www.zgxmzz.cn/, iacademicId=null, status=1, seqNo=null, journalTitleEn=Chinese Journal of Animal Science, journalPhotoCn=/LwzP9ODDP1104PfuP3+5A==, journalPhotoEn=se+5xDE//YSxHqLJ0K7Jaw==, journalFirstLetter=Z, journalRecommend=null, journalNew=null, journalCollection=null, jcrJf=null, cjcrJf=null, jcrJfStr=null, cjcrJfStr=null, submissionFirstDecision=null, sciSubjectClassification=null, casSubjectClassification=null, citeScore=null, totalCitationFrequency=null, icpCode=null, psCode=null, advertisingLicenseCode=null, copyrightInformation=null, country=null, option=, provinceCode=null, provinceName=null, collectFlag=false, interPubPlatform=, interPubPlatformUrl=null), detailUrlCn=https://castjournals.cast.org.cn/joweb/zgxmzz/CN/10.19556/j.0258-7033.20250917-05, detailUrlEn=https://castjournals.cast.org.cn/joweb/zgxmzz/EN/10.19556/j.0258-7033.20250917-05, pdfUrlCn=https://castjournals.cast.org.cn/joweb/zgxmzz/CN/PDF/10.19556/j.0258-7033.20250917-05, pdfUrlEn=https://castjournals.cast.org.cn/joweb/zgxmzz/EN/PDF/10.19556/j.0258-7033.20250917-05, aliStartDate=null, aliEndDate=null, collectionFlag=false, citedCount=null, citedUrl=null, previewStatus=0, delFlag=0, hasFullText=0, orderTime=1767456000000, fullTextJson=null, articleText=null, reference=[1]向进,王春源,罗华伦,等.柯乐猪METTL23基因多态性与繁殖性能的关联性分析[J].农业生物技术学报, 2024,32(7):1577-1586. [2]钟欣,张晖,张充,等.母猪繁殖力基因遗传育种研究进展[J].畜牧兽医学报, 2024, 55(2):438-450. [3]Guan D, Kao H. The function, regulation and therapeutic implications of the tumor suppressor protein, PML[J]. Cell Biosci, 2015, 5:60. [4]Vernier M, Bourdeau V, Gaumont-Leclerc M, et al. Regulation of E2Fs and senescence by PML nuclear bodies[J]. Genes Dev,2011, 25(1):41-50. [5]Louria-Hayon I, Alsheich-Bartok O, Levav-Cohen Y, et al.E6AP promotes the degradation of the PML tumor suppressor[J]. Cell Death Differ, 2009, 16(8):1156-1166. [6]Ebrahimian M, Mojtahedzadeh M, Bazett-Jones D, et al.Transcript isoforms of promyelocytic leukemia in mouse male and female gametes[J]. Cells Tissues Organs, 2010, 192(6):374-381. [7]Li W, Ferguson B J, Khaled W T, et al. PML depletion disrupts normal mammary gland development and skews the composition of the mammary luminal cell progenitor pool[J]. Proc Natl Acad Sci U S A, 2009, 106(12):4725-4730. [8]Spanou S, Makatounakis T, Filippopoulou C, et al. Promyelocytic leukemia protein(PML)regulates stem cell pluripotency through novel sumoylation targets[J]. Int J Mol Sci, 2025,26(3):1145. [9]Hsu K, Kao H. PML:regulation and multifaceted function beyond tumor suppression[J]. Cell Biosci, 2018, 8:5. [10]Niwa-Kawakita M, Ferhi O, Soilihi H, et al. PML is a ROS sensor activating p53 upon oxidative stress[J]. J Exp Med,2017, 214(11):3197-3206. [11]Uhlen M, Fagerberg L, Hallstrom B M, et al. Proteomics.Tissue-based map of the human proteome[J]. Science, 2015,347(6220):1260419. [12]Chamary J V, Parmley J L, Hurst L D. Hearing silence:nonneutral evolution at synonymous sites in mammals[J]. Nat Rev Genet, 2006, 7(2):98-108. [13]Zabolotskii A I, Kozlovskiy S V, Katrukha A G. The influence of the nucleotide composition of genes and gene regulatory elements on the efficiency of protein expression in escherichia coli[J]. Biochemistry(Mosc), 2023, 88(Suppl 1):S176-S191. [14]Mauger D M, Cabral B J, Presnyak V, et al. mRNA structure regulates protein expression through changes in functional halflife[J]. Proc Natl Acad Sci U S A, 2019, 116(48):24075-24083. [15]Pagani F, Baralle F E. Genomic variants in exons and introns:identifying the splicing spoilers[J]. Nat Rev Genet, 2004, 5(5):389-396. [16]Ward L D, Kellis M. Interpreting noncoding genetic variation in complex traits and human disease[J]. Nat Biotechnol, 2012,30(11):1095-1106. [17]Fukuoka T, Kawai A, Takahara T, et al. PML-RARalpha induces all-trans retinoic acid-dependent transcriptional activ-ation through interaction with MED1[J]. Transcription, 2019, 10(3):147-156. [18]Hirano S, Udagawa O. SUMOylation regulates the number and size of promyelocytic leukemia-nuclear bodies(PML-NBs)and arsenic perturbs SUMO dynamics on PML by insolubilizing PML in THP-1 cells[J]. Arch Toxicol, 2022, 96(2):545-558. [19]Meinecke I, Cinski A, Baier A, et al. Modification of nuclear PML protein by SUMO-1 regulates Fas-induced apoptosis in rheumatoid arthritis synovial fibroblasts[J]. Proc Natl Acad Sci U S A, 2007, 104(12):5073-5078. [20]Li G L, Hao W K, Hu W X. Transcription factor PU.1 and immune cell differentiation(Review)[J]. Int J Mol Med, 2020,46(6):1943-1950. [21]Salomoni P. The PML-interacting protein DAXX:histone loading gets into the picture[J]. Front Oncol, 2013, 3:152. [22]向进,吴燕,王春源,等.柯乐猪BMP7基因SNP位点与繁殖性能的关联分析[J].南方农业学报, 2024, 55(8):2495-2503. [23]向进,熊力,李维,等.柯乐猪XDH基因多态性鉴定及其对繁殖性状的影响[J].南方农业学报, 2024, 55(6):1863-1872. [24]曾辅琴,谭元成,黄雯,等.柯乐猪TAC3R基因SNP位点鉴定及其对繁殖性状的影响[J].南方农业学报, 2024, 55(5):1502-1509. [25]向进,王春源,吴燕,等.柯乐猪CRISP3基因SNP鉴定及其对繁殖性状的影响[J].浙江农业学报, 2024, 36(6):1270-1278. [26]吴燕,罗华伦,杨红文,等.柯乐猪OPN基因多态性及其与繁殖性状的关联分析[J].中国畜牧杂志, 2024, 60(8):262-269. [27]吴燕,王春源,向进,等. QDPR基因多态性对柯乐猪繁殖性状的影响[J].中国畜牧杂志, 2024, 60(9):216-223. [28]王倩倩,赵永,杨齐心,等.柯乐猪BMP7基因SNP位点鉴定及其与繁殖性状的关联分析[J].江苏农业学报, 2025,41(8):1559-1565. [29]谭元成. KISS1、TAC3和TACR3基因多态、表达对柯乐猪繁殖性状的影响[D].贵阳:贵州大学, 2023. [30]赵永,杨齐心,李维,等.柯乐猪KRT10基因多态性及其与繁殖性状的关联分析[J].中国畜牧兽医, 2024, 51(6):2508-2516. [31]杨莲,燕志宏,黄维江,等.纯种柯乐猪与巴×柯杂交猪肠道菌群结构的研究[J].动物营养学报, 2021, 33(3):1359-1371. [32]黄维江.苏太猪、巴克夏猪与柯乐猪杂交试验研究及应用[D].贵阳:贵州大学, 2020.)
中国畜牧杂志
|遗传育种
2026
, 62
(2) :
211
-217
PML基因SNP与柯乐猪繁殖性状的关联分析
全屏
[Author(id=1288182988907188447, tenantId=1146029695717560320, journalId=null, articleId=1279144335614652722, orderNo=null, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=null, email=null, emailSecond=null, emailThird=null, correspondingAuthor=null, authorType=null, ext={CN=AuthorExt(id=null, tenantId=null, journalId=1279045329999892481, articleId=1279144335614652722, authorId=1288182988907188447, language=CN, stringName=付红梅, 张游宇, 杨齐心, 杨红文, 顾丽菊, 肖礼华, 张依裕, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=null, address=null, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=null)]
付红梅, 张游宇, 杨齐心, 杨红文, 顾丽菊, 肖礼华, 张依裕
作者信息
作者简介:
付红梅(2000-),女,贵州凤冈县人,硕士研究生,研究方向:动物遗传育种与繁殖,E-mail:15185311729@163.com;
Affiliations
出版时间: 2026-01-04
doi: 10.19556/j.0258-7033.20250917-05
文章导航
试验选取240头健康的经产柯乐猪,利用PCR扩增和Sanger测序法筛选PML基因的SNP;使用SPSS 27.0软件对SNP位点与柯乐猪的繁殖性状进行关联性分析;使用在线软件RNAfold、SHEsis、STRING等对SNP位点进行生物信息学分析。结果发现PML基因第2外显子的g.59407427 A>C、第7内含子的g.59444512 G>A以及第8内含子的g.59444894 A>G。连锁不平衡分析显示g.59407427 A>C和g.59444894A>G之间存在强连锁关系(r 2 >0.88)。单倍型分析显示共存在4种单倍型和8种双倍型,群体优势双倍型为H1H1(AAAAGG)。进一步与繁殖性状关联分析显示,3个SNP对总产仔数、产活仔数、初生窝重、断奶仔猪数和断奶窝重均有显著影响,双倍型H2H3(AAAGAG)个体的产活仔数、初生窝重、断奶窝重和断奶仔猪数均高于其他双倍型,为群体有利双倍型;g.59407427 A>C没有导致氨基酸序列的改变,但mRNA二级结构发生了变化,影响了结构稳定性。在PML蛋白互作蛋白网络中主要互作蛋白DAXX、RARA、RUNX1、SUMO1、SPI1与免疫功能、发育调控和抗病性密切相关,为后续研究基因功能和调控机制提供了方向。本研究发现了3个对柯乐猪繁殖性状有显著影响的SNP,可作为柯乐猪繁殖性状选育的遗传标记进一步研究。
付红梅, 张游宇, 杨齐心, 杨红文, 顾丽菊, 肖礼华, 张依裕.
PML基因SNP与柯乐猪繁殖性状的关联分析.
中国畜牧杂志,
2026
, 62
(2)
: 211
-217
.
DOI: 10.19556/j.0258-7033.20250917-05
贵州省生猪产业科技创新项目(黔农计财[2025]9号、黔农计财[2024]33号);贵州省生猪产业技术体系健康养殖功能实验室和遵义综合试验站建设项目(GZSZCYJSTX-03、GZSZCYJSTX-07);国家重点研发计划子课题(2022YFD1100308-01);贵州省科技计划项目(黔科合平台人才[2021]5630号);贵州省科技支撑计划(黔科合支撑[2021]一般147);
参考文献
引证文献
[1]向进,王春源,罗华伦,等.柯乐猪METTL23基因多态性与繁殖性能的关联性分析[J].农业生物技术学报, 2024,32(7):1577-1586. [2]钟欣,张晖,张充,等.母猪繁殖力基因遗传育种研究进展[J].畜牧兽医学报, 2024, 55(2):438-450. [3]Guan D, Kao H. The function, regulation and therapeutic implications of the tumor suppressor protein, PML[J]. Cell Biosci, 2015, 5:60. [4]Vernier M, Bourdeau V, Gaumont-Leclerc M, et al. Regulation of E2Fs and senescence by PML nuclear bodies[J]. Genes Dev,2011, 25(1):41-50. [5]Louria-Hayon I, Alsheich-Bartok O, Levav-Cohen Y, et al.E6AP promotes the degradation of the PML tumor suppressor[J]. Cell Death Differ, 2009, 16(8):1156-1166. [6]Ebrahimian M, Mojtahedzadeh M, Bazett-Jones D, et al.Transcript isoforms of promyelocytic leukemia in mouse male and female gametes[J]. Cells Tissues Organs, 2010, 192(6):374-381. [7]Li W, Ferguson B J, Khaled W T, et al. PML depletion disrupts normal mammary gland development and skews the composition of the mammary luminal cell progenitor pool[J]. Proc Natl Acad Sci U S A, 2009, 106(12):4725-4730. [8]Spanou S, Makatounakis T, Filippopoulou C, et al. Promyelocytic leukemia protein(PML)regulates stem cell pluripotency through novel sumoylation targets[J]. Int J Mol Sci, 2025,26(3):1145. [9]Hsu K, Kao H. PML:regulation and multifaceted function beyond tumor suppression[J]. Cell Biosci, 2018, 8:5. [10]Niwa-Kawakita M, Ferhi O, Soilihi H, et al. PML is a ROS sensor activating p53 upon oxidative stress[J]. J Exp Med,2017, 214(11):3197-3206. [11]Uhlen M, Fagerberg L, Hallstrom B M, et al. Proteomics.Tissue-based map of the human proteome[J]. Science, 2015,347(6220):1260419. [12]Chamary J V, Parmley J L, Hurst L D. Hearing silence:nonneutral evolution at synonymous sites in mammals[J]. Nat Rev Genet, 2006, 7(2):98-108. [13]Zabolotskii A I, Kozlovskiy S V, Katrukha A G. The influence of the nucleotide composition of genes and gene regulatory elements on the efficiency of protein expression in escherichia coli[J]. Biochemistry(Mosc), 2023, 88(Suppl 1):S176-S191. [14]Mauger D M, Cabral B J, Presnyak V, et al. mRNA structure regulates protein expression through changes in functional halflife[J]. Proc Natl Acad Sci U S A, 2019, 116(48):24075-24083. [15]Pagani F, Baralle F E. Genomic variants in exons and introns:identifying the splicing spoilers[J]. Nat Rev Genet, 2004, 5(5):389-396. [16]Ward L D, Kellis M. Interpreting noncoding genetic variation in complex traits and human disease[J]. Nat Biotechnol, 2012,30(11):1095-1106. [17]Fukuoka T, Kawai A, Takahara T, et al. PML-RARalpha induces all-trans retinoic acid-dependent transcriptional activ-ation through interaction with MED1[J]. Transcription, 2019, 10(3):147-156. [18]Hirano S, Udagawa O. SUMOylation regulates the number and size of promyelocytic leukemia-nuclear bodies(PML-NBs)and arsenic perturbs SUMO dynamics on PML by insolubilizing PML in THP-1 cells[J]. Arch Toxicol, 2022, 96(2):545-558. [19]Meinecke I, Cinski A, Baier A, et al. Modification of nuclear PML protein by SUMO-1 regulates Fas-induced apoptosis in rheumatoid arthritis synovial fibroblasts[J]. Proc Natl Acad Sci U S A, 2007, 104(12):5073-5078. [20]Li G L, Hao W K, Hu W X. Transcription factor PU.1 and immune cell differentiation(Review)[J]. Int J Mol Med, 2020,46(6):1943-1950. [21]Salomoni P. The PML-interacting protein DAXX:histone loading gets into the picture[J]. Front Oncol, 2013, 3:152. [22]向进,吴燕,王春源,等.柯乐猪BMP7基因SNP位点与繁殖性能的关联分析[J].南方农业学报, 2024, 55(8):2495-2503. [23]向进,熊力,李维,等.柯乐猪XDH基因多态性鉴定及其对繁殖性状的影响[J].南方农业学报, 2024, 55(6):1863-1872. [24]曾辅琴,谭元成,黄雯,等.柯乐猪TAC3R基因SNP位点鉴定及其对繁殖性状的影响[J].南方农业学报, 2024, 55(5):1502-1509. [25]向进,王春源,吴燕,等.柯乐猪CRISP3基因SNP鉴定及其对繁殖性状的影响[J].浙江农业学报, 2024, 36(6):1270-1278. [26]吴燕,罗华伦,杨红文,等.柯乐猪OPN基因多态性及其与繁殖性状的关联分析[J].中国畜牧杂志, 2024, 60(8):262-269. [27]吴燕,王春源,向进,等. QDPR基因多态性对柯乐猪繁殖性状的影响[J].中国畜牧杂志, 2024, 60(9):216-223. [28]王倩倩,赵永,杨齐心,等.柯乐猪BMP7基因SNP位点鉴定及其与繁殖性状的关联分析[J].江苏农业学报, 2025,41(8):1559-1565. [29]谭元成. KISS1、TAC3和TACR3基因多态、表达对柯乐猪繁殖性状的影响[D].贵阳:贵州大学, 2023. [30]赵永,杨齐心,李维,等.柯乐猪KRT10基因多态性及其与繁殖性状的关联分析[J].中国畜牧兽医, 2024, 51(6):2508-2516. [31]杨莲,燕志宏,黄维江,等.纯种柯乐猪与巴×柯杂交猪肠道菌群结构的研究[J].动物营养学报, 2021, 33(3):1359-1371. [32]黄维江.苏太猪、巴克夏猪与柯乐猪杂交试验研究及应用[D].贵阳:贵州大学, 2020.
2026年第62卷第2期
PDF下载
34
5
引用本文
BibTeX
文章信息
doi: 10.19556/j.0258-7033.20250917-05
首发时间:2026-07-01
出版时间:2026-01-04
https://castjournals.cast.org.cn/joweb/zgxmzz/CN/10.19556/j.0258-7033.20250917-05
复制链接
引用本文
BibTeX
2种不同金属材料的力学参数
科 Family 属数 Number of genus 种数 Number of species 占总种数比例 Percentage of total species (%) 属 Genus 种数 Number of species 占总种数比例 Percentage of total species (%) 鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78 小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39 多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39 红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87 小菇属 Mycena 11 5.26 光柄菇属 Pluteus 5 2.39 红菇属 Russula 17 8.13 栓菌属 Trametes 5 2.39
关闭全屏
BibTeX
EndNote
RefWorks
TxT