中国畜牧杂志

基于16S rRNA测序和代谢组学探究子宫菌群移植对患有子宫内膜炎母牛子宫环境的影响

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中国畜牧杂志 | 遗传育种 2026,62(2): 152-163

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中国畜牧杂志 |遗传育种 2026 , 62 (2) : 152 -163

基于16S rRNA测序和代谢组学探究子宫菌群移植对患有子宫内膜炎母牛子宫环境的影响

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姜程越^1,2, 李志强³, 赵文龙^1,2, 马馨^1,2, 魏大为⁴, 房义^1,2, 吕文发^1,2

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通讯作者:

吕文发

作者简介:

姜程越（2000-），男，吉林长春人，硕士研究生，主要研究方向为动物遗传育种与繁殖，E-mail:jcy200093@outlook.com;

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出版时间: 2025-10-30 doi: 10.19556/j.0258-7033.20250729-01

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子宫内膜炎是一种常见的母牛繁殖疾病，为探究子宫菌群移植对患有子宫内膜炎母牛子宫环境的影响，本研究选择8头健康母牛，在产后30 d收集子宫灌洗液，将其灌注到8头患有子宫内膜炎的母牛子宫中进行治疗。与未接受菌群移植的8头患病牛相比，子宫菌群移植治疗提高了母牛的妊娠率。将健康（8头）、患病（8头）与移植组（8头）牛子宫液开展16S rRNA测序与代谢组学检测，结果显示：Proteobacteria是健康母牛子宫中的主要菌门，Enterococcus＿H、Phocaeicola＿A、Faecousia和Klebsiella是具有潜在治疗作用的关键菌。同时，筛选出Lauramine oxide（月桂基二甲基氧化胺）、Triptophenolide（雷酚内酯）、Gentisic acid（龙胆酸）和Phloroglucinaldehyde（间苯三酚甲醛）等具有潜在治疗作用的关键代谢物；通过Spearman相关性分析发现Thauera＿A、Metamycoplasma、Salinarimonas、Clostridium＿T、Enterococcus＿H、Turicibacter、Faecousia、Staphylococcus与关键代谢物呈正相关，Fusobacterium＿C、Porphyromonas＿A、Bacteroides＿H与关键代谢物呈负相关。由以上可知，子宫菌群移植治疗母牛子宫内膜炎具有一定效果，并改变了子宫菌群结构与代谢环境。

关键词

子宫菌群移植 / 子宫内膜炎 / 牛 / 16SrRNA / 代谢组学

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姜程越, 李志强, 赵文龙, 马馨, 魏大为, 房义, 吕文发. 基于16S rRNA测序和代谢组学探究子宫菌群移植对患有子宫内膜炎母牛子宫环境的影响. 中国畜牧杂志, 2026 , 62 (2) : 152 -163 . DOI: 10.19556/j.0258-7033.20250729-01

基金

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国家重点研发计划（2024YFD1301003）;宁夏回族自治区重点研发计划（2024BBF01007）;

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[1]Druker S A, Sicsic R, van Straten M, et al. Cytological endometritis diagnosis in primiparous versus multiparous dairy cows[J]. J Dairy Sci, 2022, 105(1):665-683.
[2]Lincke A, Drillich M, Heuwieser W. Subclinical endometritis in dairy cattle and its effect on reproductive performance-a review on recent publications[J]. Berl Munch Tierarztl Wochenschr, 2007, 120(5-6):245-250.
[3]Pérez-Báez J, Silva T V, Risco C A, et al. The economic cost of metritis in dairy herds[J]. J Dairy Sci, 2021, 104(3):3158-3168.
[4]Azawi O I. Postpartum uterine infection in cattle[J]. Anim Reprod Sci, 2008, 105(3-4):187-208.
[5]Osawa T. Predisposing factors, diagnostic and therapeutic aspects of persistent endometritis in postpartum cows[J]. J Reprod Dev , 2021, 67(5):291-299.
[6]Bretzlaff K. Rationale for treatment of endometritis in the dairy cow[J]. Vet Clin North Am Food Anim Pract, 1987, 3(3):593-607.
[7]Li H Q, Dong J S, Wang Z, et al. Development potential of selenium in the prevention and treatment of bovine endometritis[J]. Reprod Domest Anim, 2024, 59(6):e14647.
[8]Mandhwani R, Bhardwaz A, Kumar S, et al. Insights into bovine endometritis with special reference to phytotherapy[J].Vet World, 2017, 10(12):1529-1532.
[9]Yang L, Huang W Q, Yang C C, et al. Using PacBio sequencing to investigate the effects of treatment with lactic acid bacteria or antibiotics on cow endometritis[J]. Electron J Biotechn, 2021,51:67-78.
[10]Wozniak D, Cichy W, Przyslawski J, et al. The role of microbiota and enteroendocrine cells in maintaining homeostasis in the human digestive tract[J]. Adv Med Sci, 2021, 66(2):284-292.
[11]Diaz-Lundahl S, NørstebøS F, Klem T B, et al. The microbiota of uterine biopsies, cytobrush and vaginal swabs at artificial insemination in Norwegian red cows[J]. Theriogenology, 2023,209:115-125.
[12]Ducarmon Q R, Zwittink R D, Hornung B V H, et al. Gut microbiota and colonization resistance against bacterial enteric infection[J]. Microbiol Mol Biol Rev, 2019, 83(3):e00007-19.
[13]Kim K O, Gluck M. Fecal microbiota transplantation:an update on clinical practice[J]. Clin Endosc, 2019, 52(2):137-143.
[14]Yadegar A, Bar-Yoseph H, Monaghan T M, et al. Fecal microbiota transplantation:current challenges and future landscapes[J]. Clin Microbiol Rev, 2024, 37(2):e0006022.
[15]Hou K, Wu Z X, Chen X Y, et al. Microbiota in health and diseases[J]. Signal Transduct Target Ther, 2022, 7(1):135.
[16]Tuniyazi M, Zhang N. Possible therapeutic mechanisms and future perspectives of vaginal microbiota transplantation[J].Microorganisms, 2023, 11(6):1427.
[17]Quaranta G, Sanguinetti M, Masucci L. Fecal microbiota transplantation:a potential tool for treatment of human female reproductive tract diseases[J]. Front Immunol, 2019, 10:2653.
[18]Gilbert R O. The effects of endometritis on the establishment of pregnancy in cattle[J]. Reprod Fertil Dev, 2011, 24(1):252-257.
[19]Sheldon I M, Cronin J, Goetze L, et al. Defining postpartum uterine disease and the mechanisms of infection and immunity in the female reproductive tract in cattle[J]. Biol Reprod, 2009,81(6):1025-1032.
[20]Thanaboonyawat I, Pothisan S, Petyim S, et al. Pregnancy outcomes after vaginal probiotic supplementation before frozen embryo transfer:a randomized controlled study[J]. Sci Rep,2023, 13(1):11892.
[21]Li Z, Teng Y, Feng S, et al. Microbial responses and changes in metabolic products in bovine uteri infected with Staphylococcus aureus[J]. Int J Biol Macromol, 2024, 262(Pt 2):130039.
[22]Furui M, Ito A, Fukuda Y, et al. Endometrial congestion is the only hysteroscopic finding indicative of chronic endometritis[J].PLoS One, 2024, 19(6):e0303041.
[23]Halsey T M, Thomas A S, Hayase T, et al. Microbiome alteration via fecal microbiota transplantation is effective for refractory immune checkpoint inhibitor-induced colitis[J]. Sci Transl Med, 2023, 15(700):eabq4006.
[24]Guo Q, Lin H, Chen P, et al. Dynamic changes of intestinal flora in patients with irritable bowel syndrome combined with anxiety and depression after oral administration of enterobacteria capsules[J]. Bioengineered, 2021, 12(2):11885-11897.
[25]Li Y, Yang Y, Chai S, et al. Ruminal fluid transplantation accelerates rumen microbial remodeling and improves feed efficiency in yaks[J]. Microorganisms, 2023, 11(8):1964.
[26]Riquelme E, Zhang Y, Zhang L, et al. Tumor Microbiome diversity and composition influence pancreatic cancer outcomes[J]. Cell, 2019, 178(4):795-806.e712.
[27]Lee G R. The Balance of Th17 versus treg cells in autoimmunity[J]. Int J Mol Sci, 2018, 19(3):730.
[28]Zhang L, Wang L, Dai Y, et al. Effect of sow intestinal flora on the formation of endometritis[J]. Front Vet Sci, 2021, 8:663956.
[29]Cohen I, Ruff W E, Longbrake E E. Influence of immunomodulatory drugs on the gut microbiota[J]. Transl Res, 2021, 233:144-161.
[30]Cao L, Gao S, Liu J, et al. Selenomethionine protects against Escherichia coli-induced endometritis by inhibiting inflammation and necroptosis via regulating the PPAR-γ/NF-κB pathway[J]. Chem Biol Interact, 2023, 379:110532.
[31]Baïli S, Vereeck S, Jacquemyn Y. Salmonella hvittingfoss,a rare case of postoperative endometritis:A case report and review of the literature[J]. Clin Case Rep, 2024, 12(3):e8591.
[32]Galvão K N, Bicalho R C, Jeon S J. Symposium review:The uterine microbiome associated with the development of uterine disease in dairy cows[J]. J Dairy Sci, 2019, 102(12):11786-11797.
[33]Virendra A, Gulavane S U, Ahmed Z A, et al. Metagenomic analysis unravels novel taxonomic differences in the uterine microbiome between healthy mares and mares with endometritis[J]. Vet Med Sci, 2024, 10(2):e1369.
[34]Ferreira D O, Ferrari M, Morais D B, et al. Defining a panel of principal bacteria associated with endometritis[J]. JBRA Assist Reprod, 2025, 29(1):150-159.
[35]Machado V S, Bicalho R C. The infectious disease epidemiologic triangle of bovine uterine diseases[J]. Anim Reprod, 2015,12(3):450-464.
[36]Janowski T, Zdunczyk S, Baranski W. Subclinical endometritis as a cause of insemination failure in dairy cows[J]. Med Weter,2011, 67(2):79-82.
[37]Vimont A, Fernandez B, Hammami R, et al. Bacteriocin-producing enterococcus faecium LCW 44:a high potential probiotic candidate from raw camel milk[J]. Front Microbiol, 2017, 8:865.
[38]Graham K, Stack H, Rea R. Safety, beneficial and technological properties of enterococci for use in functional food applications-a review[J]. Crit Rev Food Sci Nutr, 2020, 60(22):3836-3861.
[39]Becker A, Munden S, McCabe E, et al. The endometrial microbiota-16S rRNA gene sequence signatures in healthy, pregnant and endometritis dairy cows[J]. Vet Sci, 2023, 10(3):215.
[40]Basbas C, Garzon A, Schlesener C, et al. Unveiling the microbiome during post-partum uterine infection:a deep shotgun sequencing approach to characterize the dairy cow uterine microbiome[J]. Anim microbiome, 2023, 5(1):59.
[41]Ding H, Wang Y, Li Z, et al. Baogong decoction treats endometritis in mice by regulating uterine microbiota structure and metabolites[J]. Microb Biotechnol, 2022, 15(11):2786-2799.
[42]Wandruszka R V. Detergency of specialty surfactants. surfactant science series. volume 98 edited by floyd E. Friedli(Goldschmidt Chemical Corp., Dublin, OH)[J]. J Am Chem Soc,2001, 123(38):9492.
[43]Jiang M F, Hou Q, Wu S L, et al. Metabolic profiling of triptophenolide in rat plasma, urine, bile and faeces after intragastric administration[J]. Nat Prod Res, 2024, 9(3):1-5.
[44]Pu X, Ye Q. Triptophenolide improves rheumatoid arthritis and progression by inducing macrophage toxicity[J]. J Biochem Mol Toxicol, 2025, 39(1):e70096.
[45]Abdelmawgood I A, Kotb M A, Hassan H S, et al. Gentisic acid attenuates ovalbumin-induced airway inflammation, oxidative stress, and ferroptosis through the modulation of Nrf2/HO-1and NF-κB signaling pathways[J]. Int Immunopharmacol, 2025,146:113764.
[46]Huang Y L, Huang C H, Huang Y C, et al. Anti-biofilm activities and antibiotic synergy of naturally occurring compounds against drug-resistant rapidly growing mycobacteria[J].Microbiol Spectr, 2024, 12(8):e0019924.
[47]Wang Y Y, Chen T T, Gan Z, et al. Metabolomic analysis of untargeted bovine uterine secretions in dairy cows with endometritis using ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry[J]. Res Vet Sci,2021, 139:51-58.
[48]Cao L, Gao S Y, Liu J B, et al. Selenomethionine protects against Escherichia coli-induced endometritis by inhibiting inflammation and necroptosis via regulating the PPAR-γ/NF-κB pathway[J]. Chem Biol Interact, 2023, 379:110532.
[49]Zhang J, Han L, Sheng H, et al. Genetic parameters and identification of genomic regions and candidate genes associated with vaginal discharge score in Holstein cattle based on genomic and transcriptomic analyses[J]. J Dairy Sci, 2025, 108(6):6077-6098.
[50]Chen Y, Zhao Y F, Yang J, et al. Selenium alleviates lipopolysaccharide-induced endometritis via regulating the recruitment of TLR4 into lipid rafts in mice[J]. Food Funct, 2020,11(1):200-210.."

2026年第62卷第2期

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doi: 10.19556/j.0258-7033.20250729-01

首发时间：2026-07-01
出版时间：2025-10-30

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2种不同金属材料的力学参数

科 Family	属数 Number of genus	种数 Number of species	占总种数比例 Percentage of total species (%)	属 Genus	种数 Number of species	占总种数比例 Percentage of total species (%)
鹅膏菌科Amanitaceae	2	11	5.26	鹅膏菌属 Amanita	10	4.78
小菇科 Mycenaceae	2	12	5.74	丝盖伞属 Inocybe	5	2.39
多孔菌科 Polyporaceae	8	14	6.70	蜡蘑属 Laccaria	5	2.39
红菇科 Russulaceae	3	23	11.00	小皮伞属 Marasmius	6	2.87
				小菇属 Mycena	11	5.26
				光柄菇属 Pluteus	5	2.39
				红菇属 Russula	17	8.13
				栓菌属 Trametes	5	2.39

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