Article(id=1279144330896060687, tenantId=1146029695717560320, journalId=1279045329999892481, issueId=1279144148057960780, articleNumber=PA20260323_7xA03onM, orderNo=null, doi=10.19556/j.0258-7033.20250205-04, pmid=null, cstr=null, oa=null, hot=0, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=null, receivedDateStr=null, revisedDate=null, revisedDateStr=null, acceptedDate=null, acceptedDateStr=null, onlineDate=1782901373839, onlineDateStr=2026-07-01, pubDate=1765987200000, pubDateStr=2025-12-18, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1782901373838, onlineIssueDateStr=2026-07-01, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1782901373838, creator=admin, updateTime=1782901373838, updator=admin, issue=Issue{id=1279144148057960780, tenantId=1146029695717560320, journalId=1279045329999892481, year='2026', volume='62', issue='2', pageStart='1', pageEnd='388', issueExtLink='null', onlineDate='null', pubDate='1770652800000', pubDateStr='2026-02-10', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=1, specialIssue=null, createTime=1782901330245, creator='ztmeta', updateTime=1783650204703, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1282285155289108897, tenantId=1146029695717560320, journalId=1279045329999892481, issueId=1279144148057960780, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1282285155289108898, tenantId=1146029695717560320, journalId=1279045329999892481, issueId=1279144148057960780, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=206, endPage=210, ext={CN=ArticleExt(id=1284209635267678524, articleId=1279144330896060687, tenantId=1146029695717560320, journalId=1279045329999892481, language=CN, title=绵羊不同组织细胞中Marco基因启动子甲基化与转录表达水平的关联性分析, columnId=1282278062347764011, journalTitle=中国畜牧杂志, columnName=遗传育种, runingTitle=null, highlight=null, articleAbstract=本研究旨在揭示绵羊胶原样结构巨噬细胞受体（Macrophage Receptor with Collagenous Structure,Marco）组织特异性表达的机制，探讨Marco表达水平与其启动子甲基化的相关性。通过检测肺组织、肺泡巨噬细胞及脾脏细胞中Marco mRNA表达量，利用亚硫酸氢盐测序法（BSP）测定Marco启动子甲基化水平，进一步测定Marco启动子活性，分析DNA甲基化酶处理对Marco启动子活性的影响。结果显示，脾脏组织中Marco mRNA表达量最高，肺脏次之，肺泡巨噬细胞表达量极低；甲基化测定结果显示，Marco启动子甲基化水平在肺泡巨噬细胞最高（96.4%），肺脏处于中等（60.7%），脾脏最低（55.4%）。荧光素酶活性检测表明，Marco启动子具有较高的活性，且启动子区域甲基化抑制Marco启动子活性；初步证实Marco启动子甲基化水平与其在组织中的限制表达存在密切联系。本研究为后续深入探究Marco基因的转录调控机理提供了重要参考依据。, authors=薛嘉熹¹, 王钢², 顾兰英¹, 张秦川¹, 赵广新¹, 孙延鸣¹, 贺笋², 张彦兵¹, authorsList=薛嘉熹, 王钢, 顾兰英, 张秦川, 赵广新, 孙延鸣, 贺笋, 张彦兵, authorCompany=1.石河子大学动物科技学院;
2.天康制药股份有限公司, correspAuthors=贺笋, authorNote=薛嘉熹（2001-），男，甘肃金昌人，本科生，研究方向为动物医学，E-mail:19190249702@163.com; 王钢（1986-），男，湖南人，硕士，兽医师，主要从事临床兽医学研究，E-mail:wanggang@tecon-bio.com;, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=WMtIv7tQvlbWBq+zg6n8JQ==, pdfFileSize=2338368, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=国家自然科学基金项目（32360901）;兵团指导性科技计划项目（2023ZD042）;新疆维吾尔自治区两区项目（2023LQ03003）;新疆维吾尔自治区科学基金项目（2022D01B197）;石河子大学高层次人才科研启动项目（RCZK202043）;)}, authors=null, keywords=[Keyword(id=1284217186793263351, tenantId=1146029695717560320, journalId=1279045329999892481, articleId=1279144330896060687, language=CN, orderNo=1, keyword=DNA甲基化), Keyword(id=1284217186919092472, tenantId=1146029695717560320, 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