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[43]曹婉怡.猪全基因组CRISPR敲除文库的构建及在呼肠孤病毒宿主因子筛选中的应用[D].广州:华南农业大学, 2021.
[44]崔欢,张诚,张春茂,等. H7N9亚型禽流感病毒研究进展[J].中国兽医学报, 2020(40):1871-1875.
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[47]Semler B L, Peng G, Liu T, et al. A genome-wide CRISPR screening uncovers that TOB1 acts as a key host factor for FMDV infection via both IFN and EGFR mediated pathways[J]. PLoS Pathogens, 2024, 20(3):e1012104.
[48]曹靖宝,秦波.奶牛育种基因标记技术及在黑龙江省应用的前景展望[J].中国奶牛, 2009(7):25-28.
[49]Wang L M, Ma S, Ding Q, et al. CRISPR/Cas9-mediated MSTN gene editing induced mitochondrial alterations in C2C12myoblast cells[J]. Electron J Biotechnol, 2019, 40:30-39.
[50]郝晋杨.基于CRISPR/Cas9牛肾脏细胞全基因组敲除文库的构建[D].呼和浩特:内蒙古大学, 2023.
[51]Tan W S, Rong E, Dry I, et al. Validation of candidate host cell entry factors for bovine herpes virus type-1 based on a genomewide CRISPR knockout screen[J]. Viruses, 2024, 16(2):297.
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[67]Chardon F M, Mcdiarmid T A, Page N F, et al. Multiplex,single-cell CRISPRa screening for cell type specific regulatory elements[J]. Nat Commun, 2024, 15(1):8209.
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[69]Korkmaz G, Lopes R, Ugalde A P, et al. Functional genetic screens for enhancer elements in the human genome using CRISPR-Cas9[J]. Nat Biotechnol, 2016, 34(2):192-198..")
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CRISPR/Cas9文库筛选技术及其在畜禽研究中的应用
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中国畜牧杂志 |综述 2026 , 62 (2) : 112 -120
CRISPR/Cas9文库筛选技术及其在畜禽研究中的应用
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王颖慧1,2, 连正兴2
作者信息
    1.中国农业大学烟台研究院;
    2.中国农业大学动物科学技术学院
通讯作者:
连正兴
作者简介:
王颖慧(2002-),女,山西晋中人,硕士研究生,主要从事动物遗传育种研究,E-mail:18303440158@163.com;
  • Affiliations
    出版时间: 2025-12-19 doi: 10.19556/j.0258-7033.20250204-01
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    CRISPR/Cas9基因编辑技术因其操作简便、高效和准确等优势,成为最流行的基因编辑技术之一,在畜禽遗传改良领域得到广泛应用。CRISPR/Cas9文库筛选技术作为新一代基因编辑技术,在解决畜禽育种、疾病控制等方面都有诸多应用,特别是在猪、牛、羊、鸡等畜禽的遗传育种和疾病防治研究中展现出巨大潜力。通过CRISPR/Cas9文库筛选,能够筛选出与多种病毒及病原体相关的抗性基因,同时挖掘出控制畜禽生长速度、肉质品质等重要性状的关键因子。本文介绍了CRISPR/Cas9文库筛选技术的基本原理及方法流程,综述了CRISPR/Cas9文库筛选技术对猪、牛、羊、鸡等畜禽在遗传改良、疾病防控方面的研究进展,以期为今后深入挖掘CRISPR/Cas9文库筛选技术的应用价值提供参考。
    基因编辑技术  /  CRISPR/Cas9文库筛选技术  /  畜禽遗传育种  /  疾病防治
    王颖慧, 连正兴. CRISPR/Cas9文库筛选技术及其在畜禽研究中的应用. 中国畜牧杂志, 2026 , 62 (2) : 112 -120 . DOI: 10.19556/j.0258-7033.20250204-01

      中国国家重点研发项目(2021YFD1200902);国家自然科学基金(32272853);

    参考文献 引证文献
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    [64]孙佳琳.鸡滑液囊支原体基因组表达文库的构建及体内诱导基因的筛选与鉴定[D].银川:宁夏大学, 2023.
    [65]魏宇航. CRISPR/Cas9脱靶效应产生机制的研究进展[J].中外医学研究杂志, 2023, 2(11):154-156.
    [66]樊祥瑞,王俊燕,梁丽亚,等.基于CRISPR/Cas系统的多重基因编辑与调控技术[J].生物工程学报, 2023(39):2449-2464.
    [67]Chardon F M, Mcdiarmid T A, Page N F, et al. Multiplex,single-cell CRISPRa screening for cell type specific regulatory elements[J]. Nat Commun, 2024, 15(1):8209.
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    [69]Korkmaz G, Lopes R, Ugalde A P, et al. Functional genetic screens for enhancer elements in the human genome using CRISPR-Cas9[J]. Nat Biotechnol, 2016, 34(2):192-198.."
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    占总种数比例
    Percentage of
    total species (%)

    Genus
    种数
    Number of
    species
    占总种数比例
    Percentage of total
    species (%)
    鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
    小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
    多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
    红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
    小菇属 Mycena 11 5.26
    光柄菇属 Pluteus 5 2.39
    红菇属 Russula 17 8.13
    栓菌属 Trametes 5 2.39
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