中国畜牧杂志

CRISPR/Cas9文库筛选技术及其在畜禽研究中的应用

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王颖慧, 连正兴

中国畜牧杂志 | 综述 2026,62(2): 112-120

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中国畜牧杂志 |综述 2026 , 62 (2) : 112 -120

CRISPR/Cas9文库筛选技术及其在畜禽研究中的应用

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王颖慧, 连正兴

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连正兴

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王颖慧（2002-），女，山西晋中人，硕士研究生，主要从事动物遗传育种研究，E-mail:18303440158@163.com;

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出版时间: 2025-12-19 doi: 10.19556/j.0258-7033.20250204-01

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CRISPR/Cas9基因编辑技术因其操作简便、高效和准确等优势，成为最流行的基因编辑技术之一，在畜禽遗传改良领域得到广泛应用。CRISPR/Cas9文库筛选技术作为新一代基因编辑技术，在解决畜禽育种、疾病控制等方面都有诸多应用，特别是在猪、牛、羊、鸡等畜禽的遗传育种和疾病防治研究中展现出巨大潜力。通过CRISPR/Cas9文库筛选，能够筛选出与多种病毒及病原体相关的抗性基因，同时挖掘出控制畜禽生长速度、肉质品质等重要性状的关键因子。本文介绍了CRISPR/Cas9文库筛选技术的基本原理及方法流程，综述了CRISPR/Cas9文库筛选技术对猪、牛、羊、鸡等畜禽在遗传改良、疾病防控方面的研究进展，以期为今后深入挖掘CRISPR/Cas9文库筛选技术的应用价值提供参考。

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基因编辑技术 / CRISPR/Cas9文库筛选技术 / 畜禽遗传育种 / 疾病防治

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王颖慧, 连正兴. CRISPR/Cas9文库筛选技术及其在畜禽研究中的应用. 中国畜牧杂志, 2026 , 62 (2) : 112 -120 . DOI: 10.19556/j.0258-7033.20250204-01

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中国国家重点研发项目（2021YFD1200902）;国家自然科学基金（32272853）;

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2026年第62卷第2期

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doi: 10.19556/j.0258-7033.20250204-01

首发时间：2026-07-01
出版时间：2025-12-19

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2种不同金属材料的力学参数

科 Family	属数 Number of genus	种数 Number of species	占总种数比例 Percentage of total species (%)	属 Genus	种数 Number of species	占总种数比例 Percentage of total species (%)
鹅膏菌科Amanitaceae	2	11	5.26	鹅膏菌属 Amanita	10	4.78
小菇科 Mycenaceae	2	12	5.74	丝盖伞属 Inocybe	5	2.39
多孔菌科 Polyporaceae	8	14	6.70	蜡蘑属 Laccaria	5	2.39
红菇科 Russulaceae	3	23	11.00	小皮伞属 Marasmius	6	2.87
				小菇属 Mycena	11	5.26
				光柄菇属 Pluteus	5	2.39
				红菇属 Russula	17	8.13
				栓菌属 Trametes	5	2.39

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