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tenantId=1146029695717560320, journalId=1279045329999892481, language=CN, title=玉米赤霉烯酮降解菌的分离鉴定、益生特性及其对中毒小鼠的缓解作用研究, columnId=1282278072095326533, journalTitle=中国畜牧杂志, columnName=动物健康, runingTitle=null, highlight=null, articleAbstract=本研究旨在分离筛选出具有体外降解玉米赤霉烯酮(Zearalenone,ZEN),且具备良好益生性的枯草芽孢杆菌,并评估其缓解小鼠ZEN中毒症状的能力,为其作为饲料添加剂和霉菌毒素脱毒菌剂提供参考依据。从牡丹根际土壤样品中,分离纯化对ZEN具有降解能力的益生菌并探究菌株益生性能。将30只雌鼠随机分为3组,每组10只,每组平均体重25~28 g,对照组小鼠灌服生理盐水,ZEN组灌服ZEN标准品稀释液(50 mg/kg BW),H-1组灌服ZEN标准品稀释液(50 mg/kg BW)与H-1培养液(1×10~8 CFU/mL),每只小鼠灌服剂量为0.2 mL,检测各组生长性能、脏器指标和肝脏与生殖系统相关指标。结果表明:枯草芽孢杆菌H-1玉米赤霉烯酮体外降解率可达88.3%,并且有较好的益生性与安全性。与ZEN组相比,H-1菌株对ZEN造成的生长性能影响有改善作用(P<0.05);同时,与ZEN组相比,H-1组子宫卵巢系数降低(P<0.05),促卵泡生成素(FSH)、促黄体生成素(LH)、雌二醇(E 2 )含量升高(P>0.05),谷丙转氨酶(ALT)、谷草转氨酶(AST)水平降低(P<0.05)。分离所得枯草芽孢杆菌H-1具有体外降解玉米赤霉烯酮的能力与良好的益生性能,可缓解玉米赤霉烯酮中毒对小鼠肝脏及生殖系统造成的损伤。, authors=张启航1 , 吴慧昊2 , 陈文燕1 , 周瑞1,3 , 李耀东1 , 臧荣鑫1 , 徐红伟1 , authorsList=张启航, 吴慧昊, 陈文燕, 周瑞, 李耀东, 臧荣鑫, 徐红伟, authorCompany=1.西北民族大学生命科学与工程学院; 2.西北民族大学实验教学部; 3.西北民族大学生物技术与生物工程国家民委重点实验室, 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Food Sci Biotechnol, 2017, 26:1641-1648. [20]Pełka K, Hafeez A B, Worobo R W, et al. Probiotic potential of Bacillus Isolates from Polish Bee Pollen and Bee Bread[J].Probiotics Antimicrob, 2023:1-14. [21]刘洋,郭宇星,潘道东. 4种乳酸菌体外抗氧化能力的比较研究[J].食品科学, 2012, 33(11):25-29. [22]Isenring J, Geirnaert A, Lacroix C, et al. Bistable auto-aggregation phenotype in Lactiplantibacillus plantarum emerges after cultivation in in vitro colonic microbiota[J]. BMC Microbiol,2021, 21:1-13. [23]Lei Y P, Zhao L H, Ma Q G, et al. Degradation of zearalenone in swine feed and feed ingredients by Bacillus subtilis ANSB01G[J]. World Mycotoxin J, 2014, 7(2):143-151. [24]王泽贤,赵宇楠,贾丹丹,等.蜜环菌Am-07-22发酵对玉米赤霉烯酮降解效果影响及机理初探[J].食品工业科技,2024, 45(1):162-169. [25]游龙,韩茵,张凯,等.芽孢杆菌产胞外酶的活性分析及其对凡纳滨对虾的作用[J].中国海洋大学学报(自然科学版),2018, 48(3):80-87. [26]刘之园,贾俊霞,姜昊蔚,等.耐酸耐胆盐益生菌的筛选及其益生特性研究[J].中国酿造, 2020, 39(11):103-108. [27]陈仪婷,张红星,谢远红,等.降胆固醇乳酸菌的筛选鉴定及其耐酸耐胆盐性能研究[J].食品与发酵工业, 2018, 44(5):29-33. [28]Ru X, Zhang C C, Yuan Y H, et al. Bile salt hydrolase activity is present in nonintestinal lactic acid bacteria at an intermediate level[J]. Appl Microbiol Biotechnol, 2019, 103:893-902. [29]Zhang L, Ma H, Kulyar M F A, et al. Complete genome analysis of Lactobacillus fermentum YLF016 and its probiotic characteristics[J]. Microb Pathogenesis, 2022, 162:105212. [30]Wadström T, Andersson K, Sydow M, et al.Surface properties of lactobacilli isolated fromthe small intestine of pigs[J]. J Appl Bacteriol, 2010, 62(6):513-520. [31]李龙.藏鸡源乳酸菌和红景天对肉鸡生产性能、腹水敏感性和肠道功能的影响[D].杨凌:西北农林科技大学, 2015. [32]石超.传统发酵牦牛酸奶中降胆固醇乳酸菌的筛选及其对高脂模型大鼠的益生作用[D].兰州:兰州大学, 2014. [33]代玉明,黄涛,熊涛.鸡源凝结芽孢杆菌的筛选鉴定及益生性能评价[J].南昌大学学报(理科版), 2022, 46(4):464-472, 480. [34]王惋,侯俊财,于彤,等.具有抗氧化和抑菌能力的益生性乳酸菌筛选及鉴定[J].食品与发酵工业, 2020, 46(3):43-49. [35]陈曦,李欣翼,陈发顺,等. 5株解淀粉芽孢杆菌的益生特性及抑菌活性研究[J].中国畜牧兽医, 2023, 50(5):2082-2091. [36]陈童锦悦,覃绍敏,韦珏,等.竹鼠源产纤维素酶枯草芽孢杆菌的生物学特性研究及安全性初步评价[J].中国畜牧杂志, 2024, 60(3):201-206. [37]Bai J, Zhou Y, Luo X, et al. Roles of stress response-related signaling and its contribution to the toxicity of zearalenone in mammals[J]. Compr Rev Food Sci F, 2022, 21(4):3326-3345. [38]朱根生,夏苏干,佘进进,等.白藜芦醇对玉米赤霉烯酮致小鼠肝脏氧化损伤及炎症的保护作用[J].中国畜牧兽医,2021, 48(11):4254-4261. [39]刘淑杰,邓波,吴杰,等. ZEN污染饲料中添加植物炭黑对大鼠脏器组织、抗氧化指标及免疫功能的影响[J].中国畜牧杂志, 2022, 58(1):225-230. [40]朱炜凤.金丝桃苷通过ROS/Nrf2/Cyt-c缓解玉米赤霉烯酮暴露诱导小鼠脾细胞凋亡的研究[D].哈尔滨:东北农业大学, 2022. [41]张琼琼,王亚男,唐彧,等.芦丁对玉米赤霉烯酮诱导的雌鼠生殖器官损伤的缓解作用[J].中国畜牧杂志, 2023, 59(5):278-284. [42]朱云迪,杨青,王新芳,等. 3种中药成分对玉米赤霉烯酮致小鼠肝脏损伤的缓解作用[J].中国畜牧兽医, 2024, 51(1):434-442.)
中国畜牧杂志
|动物健康
2026
, 62
(2) :
317
-324
玉米赤霉烯酮降解菌的分离鉴定、益生特性及其对中毒小鼠的缓解作用研究
全屏
张启航1 , 吴慧昊2 , 陈文燕1 , 周瑞1,3 , 李耀东1 , 臧荣鑫1 , 徐红伟1
作者信息
1.西北民族大学生命科学与工程学院; 2.西北民族大学实验教学部; 3.西北民族大学生物技术与生物工程国家民委重点实验室
通讯作者:
臧荣鑫
作者简介:
张启航(1999-),男,山东济南人,硕士,主要从事肠道菌群与健康研究,E-mail:1614427745@qq.com;
Affiliations
出版时间: 2025-12-19
doi: 10.19556/j.0258-7033.20250120-04
文章导航
本研究旨在分离筛选出具有体外降解玉米赤霉烯酮(Zearalenone,ZEN),且具备良好益生性的枯草芽孢杆菌,并评估其缓解小鼠ZEN中毒症状的能力,为其作为饲料添加剂和霉菌毒素脱毒菌剂提供参考依据。从牡丹根际土壤样品中,分离纯化对ZEN具有降解能力的益生菌并探究菌株益生性能。将30只雌鼠随机分为3组,每组10只,每组平均体重25~28 g,对照组小鼠灌服生理盐水,ZEN组灌服ZEN标准品稀释液(50 mg/kg BW),H-1组灌服ZEN标准品稀释液(50 mg/kg BW)与H-1培养液(1×10~8 CFU/mL),每只小鼠灌服剂量为0.2 mL,检测各组生长性能、脏器指标和肝脏与生殖系统相关指标。结果表明:枯草芽孢杆菌H-1玉米赤霉烯酮体外降解率可达88.3%,并且有较好的益生性与安全性。与ZEN组相比,H-1菌株对ZEN造成的生长性能影响有改善作用(P<0.05);同时,与ZEN组相比,H-1组子宫卵巢系数降低(P<0.05),促卵泡生成素(FSH)、促黄体生成素(LH)、雌二醇(E 2 )含量升高(P>0.05),谷丙转氨酶(ALT)、谷草转氨酶(AST)水平降低(P<0.05)。分离所得枯草芽孢杆菌H-1具有体外降解玉米赤霉烯酮的能力与良好的益生性能,可缓解玉米赤霉烯酮中毒对小鼠肝脏及生殖系统造成的损伤。
枯草芽孢杆菌
/
分离鉴定
/
益生性
/
玉米赤霉烯酮
张启航, 吴慧昊, 陈文燕, 周瑞, 李耀东, 臧荣鑫, 徐红伟.
玉米赤霉烯酮降解菌的分离鉴定、益生特性及其对中毒小鼠的缓解作用研究.
中国畜牧杂志,
2026
, 62
(2)
: 317
-324
.
DOI: 10.19556/j.0258-7033.20250120-04
参考文献
引证文献
[1]Ropejko K, Twarużek M. Zearalenone and its metabolites—General overview, occurrence, and toxicity[J]. Toxins, 2021,13(1):35. [2]Gao X, Xiao Z H, Liu M, et al. Dietary silymarin supplementation alleviates zearalenone-induced hepatotoxicity and reproductive toxicity in rats[J]. J Nutr, 2018, 148(8):1209-1216. [3]刘同威,李爱科,孙肖明,等.饲料原料中霉菌毒素对动物的危害及其检测和消减措施[J].中国兽医学报, 2024, 44(3):632-643, 650. [4]刘建高,厉学武,彭哲,等. 2021年我国饲料原料中常见霉菌毒素污染情况调查[J].中国家禽, 2023, 45(10):70-75. [5]Pirouz A, Selamat J, Iqbal S, et al. The use of innovative and efficient nanocomposite(magnetic graphene oxide)for the reduction on of Fusarium mycotoxins in palm kernel cake[J].Sci Rep, 2017, 7(1):e12453. [6]叶盛群.腐殖酸钠去除霉菌毒素效果的研究[D].成都:四川农业大学, 2009. [7]MArkovićM, DakovićA, Rottinghaus G E, et al. Adsorption of the mycotoxin zearalenone by clinoptilolite and phillipsite zeolites treated with cetylpyridinium surfactant[J]. Colloids Surf B, 2017, 151:324-332. [8]Polak M, Gajecki M, Kulik T, et al. The evaluation of the efficacy of sodium carbonate as zearalenone destructor in feeding stuffs[J]. Pol J Vet Sci, 2009, 12(1):103. [9]王晓璐,罗会颖,姚斌.饲料霉菌毒素的生物降解研究进展[J].动物营养学报, 2022, 34(10):6152-6159. [10]文钧,熊东炜,龙淼.玉米赤霉烯酮降解菌的筛选、培养条件优化及降解酶挖掘[J].动物营养学报, 2023, 35(11):7422-7432. [11]王悦.有效清除玉米赤霉烯酮并正向调节肠道菌群的菌株分离与研究[D].西安:西北大学, 2018. [12]耿凯伟.解淀粉芽孢杆菌H6发酵条件优化及其降解玉米赤霉烯酮效果评价[D].郑州:河南农业大学, 2023. [13]沈萍,陈向东.微生物学实验(第5版)[M].北京:高等教育出版社, 2018. [14]国家药典委员会.中华人民共和国药典[M].北京:中国医药科技出版社, 2015. [15]刘晨.玉米赤霉烯酮降解菌的筛选、鉴定及降解特性研究[D].郑州:河南工业大学, 2022. [16]赵斌,林会,何绍江.微生物学实验[M].北京:科学出版社,2014. [17]Guo Z, Wang J, Yan L, et al. In vitro comparison of probiotic properties of Lactobacillus casei Zhang, a potential new probiotic, with selected probiotic strains[J]. LWT-Food Sci Technol, 2009, 42(10):1640-1646. [18]于秀菊,胡燕姣,孙铮,等.羊驼源地衣芽孢杆菌SXAU08的益生功能评价及其对小鼠肠道菌群的影响[J].动物营养学报, 2024, 36(10):6780-6790. [19]Jeon H L, Lee N K, Yang S J, et al. Probiotic characterization of Bacillus subtilis P223 isolated from kimchi[J]. Food Sci Biotechnol, 2017, 26:1641-1648. [20]Pełka K, Hafeez A B, Worobo R W, et al. Probiotic potential of Bacillus Isolates from Polish Bee Pollen and Bee Bread[J].Probiotics Antimicrob, 2023:1-14. [21]刘洋,郭宇星,潘道东. 4种乳酸菌体外抗氧化能力的比较研究[J].食品科学, 2012, 33(11):25-29. [22]Isenring J, Geirnaert A, Lacroix C, et al. Bistable auto-aggregation phenotype in Lactiplantibacillus plantarum emerges after cultivation in in vitro colonic microbiota[J]. BMC Microbiol,2021, 21:1-13. [23]Lei Y P, Zhao L H, Ma Q G, et al. Degradation of zearalenone in swine feed and feed ingredients by Bacillus subtilis ANSB01G[J]. World Mycotoxin J, 2014, 7(2):143-151. [24]王泽贤,赵宇楠,贾丹丹,等.蜜环菌Am-07-22发酵对玉米赤霉烯酮降解效果影响及机理初探[J].食品工业科技,2024, 45(1):162-169. [25]游龙,韩茵,张凯,等.芽孢杆菌产胞外酶的活性分析及其对凡纳滨对虾的作用[J].中国海洋大学学报(自然科学版),2018, 48(3):80-87. [26]刘之园,贾俊霞,姜昊蔚,等.耐酸耐胆盐益生菌的筛选及其益生特性研究[J].中国酿造, 2020, 39(11):103-108. [27]陈仪婷,张红星,谢远红,等.降胆固醇乳酸菌的筛选鉴定及其耐酸耐胆盐性能研究[J].食品与发酵工业, 2018, 44(5):29-33. [28]Ru X, Zhang C C, Yuan Y H, et al. Bile salt hydrolase activity is present in nonintestinal lactic acid bacteria at an intermediate level[J]. Appl Microbiol Biotechnol, 2019, 103:893-902. [29]Zhang L, Ma H, Kulyar M F A, et al. Complete genome analysis of Lactobacillus fermentum YLF016 and its probiotic characteristics[J]. Microb Pathogenesis, 2022, 162:105212. [30]Wadström T, Andersson K, Sydow M, et al.Surface properties of lactobacilli isolated fromthe small intestine of pigs[J]. J Appl Bacteriol, 2010, 62(6):513-520. [31]李龙.藏鸡源乳酸菌和红景天对肉鸡生产性能、腹水敏感性和肠道功能的影响[D].杨凌:西北农林科技大学, 2015. [32]石超.传统发酵牦牛酸奶中降胆固醇乳酸菌的筛选及其对高脂模型大鼠的益生作用[D].兰州:兰州大学, 2014. [33]代玉明,黄涛,熊涛.鸡源凝结芽孢杆菌的筛选鉴定及益生性能评价[J].南昌大学学报(理科版), 2022, 46(4):464-472, 480. [34]王惋,侯俊财,于彤,等.具有抗氧化和抑菌能力的益生性乳酸菌筛选及鉴定[J].食品与发酵工业, 2020, 46(3):43-49. [35]陈曦,李欣翼,陈发顺,等. 5株解淀粉芽孢杆菌的益生特性及抑菌活性研究[J].中国畜牧兽医, 2023, 50(5):2082-2091. [36]陈童锦悦,覃绍敏,韦珏,等.竹鼠源产纤维素酶枯草芽孢杆菌的生物学特性研究及安全性初步评价[J].中国畜牧杂志, 2024, 60(3):201-206. [37]Bai J, Zhou Y, Luo X, et al. Roles of stress response-related signaling and its contribution to the toxicity of zearalenone in mammals[J]. Compr Rev Food Sci F, 2022, 21(4):3326-3345. [38]朱根生,夏苏干,佘进进,等.白藜芦醇对玉米赤霉烯酮致小鼠肝脏氧化损伤及炎症的保护作用[J].中国畜牧兽医,2021, 48(11):4254-4261. [39]刘淑杰,邓波,吴杰,等. ZEN污染饲料中添加植物炭黑对大鼠脏器组织、抗氧化指标及免疫功能的影响[J].中国畜牧杂志, 2022, 58(1):225-230. [40]朱炜凤.金丝桃苷通过ROS/Nrf2/Cyt-c缓解玉米赤霉烯酮暴露诱导小鼠脾细胞凋亡的研究[D].哈尔滨:东北农业大学, 2022. [41]张琼琼,王亚男,唐彧,等.芦丁对玉米赤霉烯酮诱导的雌鼠生殖器官损伤的缓解作用[J].中国畜牧杂志, 2023, 59(5):278-284. [42]朱云迪,杨青,王新芳,等. 3种中药成分对玉米赤霉烯酮致小鼠肝脏损伤的缓解作用[J].中国畜牧兽医, 2024, 51(1):434-442.
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doi: 10.19556/j.0258-7033.20250120-04
首发时间:2026-07-01
出版时间:2025-12-19
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2种不同金属材料的力学参数
科 Family 属数 Number of genus 种数 Number of species 占总种数比例 Percentage of total species (%) 属 Genus 种数 Number of species 占总种数比例 Percentage of total species (%) 鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78 小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39 多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39 红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87 小菇属 Mycena 11 5.26 光柄菇属 Pluteus 5 2.39 红菇属 Russula 17 8.13 栓菌属 Trametes 5 2.39
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