中国畜牧杂志

武定鸡FABP6基因克隆及生物信息学分析

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邱文才, 苏航, 杨明华, 赵素梅, 潘洪彬, 黄英, 李永能

中国畜牧杂志 | 畜牧生物技术 2026,62(2): 345-353

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中国畜牧杂志 |畜牧生物技术 2026 , 62 (2) : 345 -353

武定鸡FABP6基因克隆及生物信息学分析

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邱文才, 苏航, 杨明华, 赵素梅, 潘洪彬, 黄英, 李永能

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邱文才，男，云南昆明人，硕士，主要从事动物营养与饲料科学研究，E-mail:qiuqiudx@163.com;

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出版时间: 2025-12-16 doi: 10.19556/j.0258-7033.20241219-03

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本研究旨在克隆武定鸡脂肪酸结合蛋白6(FABP6)基因CDS区序列并进行生物信息学分析，为后续研究FABP6基因对武定鸡脂肪代谢与沉积的影响提供参考。以武定鸡为试验对象，设计FABP6引物、提取总RNA、反转录、PCR扩增并克隆其编码区序列，并进行同源性比对及系统进化树构建；运用生物信息学软件对其编码蛋白的理化性质、亲/疏水性、跨膜区、信号肽、修饰结构、保守结构域及二、三级结构进行预测。结果显示：武定鸡FABP6基因CDS序列全长387 bp,FABP6蛋白等电点7.82，平均疏水指数-0.566，为亲水蛋白，不存在信号肽和跨膜区，主要定位于细胞质。FABP6蛋白被14个磷酸化位点修饰，无糖基化位点，主要由α-螺旋、延伸链和无规卷曲构成。武定鸡FABP6与原鸡、火鸡、珍珠鸡、雪鸡、环颈雉鸡、鹌鹑、鸿雁、棕硬尾鸭、天鹅和绿头鸭基因同源性分别为99.2%、96.6%、96.4%、96.1%、96.1%、95.9%、90.2%、89.7%、89.7%和89.4%。FABP6蛋白与FABP2、FABP3、FABP4、FABP5、FABP7、FABP9、NR1H4、RXRA、SLC10A2和SNX24蛋白存在互作关系。本研究明确了FABP6基因在武定鸡脂肪代谢与沉积中的核心作用，为其分子机制的深入解析奠定了理论基础，也为我国优质地方鸡种的肉质改良与高效新品种培育提供了候选基因。

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武定鸡 / FABP6基因 / 基因克隆 / 生物信息学分析

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邱文才, 苏航, 杨明华, 赵素梅, 潘洪彬, 黄英, 李永能. 武定鸡FABP6基因克隆及生物信息学分析. 中国畜牧杂志, 2026 , 62 (2) : 345 -353 . DOI: 10.19556/j.0258-7033.20241219-03

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国家十四五重点研发计划项目（2022YFD1601905）;

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2026年第62卷第2期

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doi: 10.19556/j.0258-7033.20241219-03

首发时间：2026-07-01
出版时间：2025-12-16

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2种不同金属材料的力学参数

科 Family	属数 Number of genus	种数 Number of species	占总种数比例 Percentage of total species (%)	属 Genus	种数 Number of species	占总种数比例 Percentage of total species (%)
鹅膏菌科Amanitaceae	2	11	5.26	鹅膏菌属 Amanita	10	4.78
小菇科 Mycenaceae	2	12	5.74	丝盖伞属 Inocybe	5	2.39
多孔菌科 Polyporaceae	8	14	6.70	蜡蘑属 Laccaria	5	2.39
红菇科 Russulaceae	3	23	11.00	小皮伞属 Marasmius	6	2.87
				小菇属 Mycena	11	5.26
				光柄菇属 Pluteus	5	2.39
				红菇属 Russula	17	8.13
				栓菌属 Trametes	5	2.39

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