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2 University of Shanghai for Science and Technology, Shanghai 200093, correspAuthors=null, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1320326752824570269, articleId=1320326752098955676, tenantId=1146029695717560320, journalId=1296801528146919473, language=CN, title=基于荟萃分析的乳及乳制品中假单胞菌属的生长动力学研究, columnId=1297134032135938673, journalTitle=中国乳业, columnName=质量安全, runingTitle=null, highlight=null, articleAbstract=假单胞菌属是生乳中最常见的嗜冷菌属之一,为探讨影响假单胞菌属动力学参数的主要因素,本研究以定量整合微生物动力学参数并量化菌株变异性的方法开展荟萃分析。通过统计750 余条假单胞菌属在不同条件下的生长参数,并基于平方根模型预测其最低生长温度(Tmin),结果表明,假单胞菌属整体Tmin为-4.06 ℃,其中菌株1248和E52在培养基中的Tmin分别为-5.38 ℃和-6.36 ℃。在低温环境下,UHT牛乳中高初始接菌量(Y0)显著缩短了迟滞期(λ),使微生物即使在较低温度下也能更快进入对数生长期。在温度(T)、pH值、水分活度(Aw)和NaCl浓度的共同作用下,最大比生长速率(μmax)分布范围为0.04~1.63 h-1。此外,NaCl浓度(1.0%~7.5%)对假单胞菌属的生长具有显著抑制作用。本研究可为乳及乳制品生产过程中的安全性、生产优化和质量控制提供重要参考依据。, authors=江春铃1, 祁兵1, 朱华剑2, 马颖清1, 陈柔含1, 杨晓君1, 邓波1,*, authorsList=江春铃, 祁兵, 朱华剑, 马颖清, 陈柔含, 杨晓君, 邓波, authorCompany=1 上海市农产品质量安全中心, 上海 201708;
2 上海理工大学, 上海 200093, correspAuthors=*邓波(1982-),男,湖北汉川人,硕士,高级兽医师,研究方向为农产品质量安全预测预警和风险评估。, authorNote=江春铃(1998-),女,江苏无锡人,硕士,助理畜牧师,研究方向为食用农产品微生物检测;祁兵(1982-),男,江苏宿迁人,硕士,助理兽医师,研究方向为农产品质量安全预测预警和风险评估;朱华剑(1998-),男,江苏南通人,硕士,助理畜牧师,研究方向为食源性致病菌控制;马颖清(1984-),女,江苏射阳人,硕士,畜牧师,研究方向为农产品质量安全和品质;陈柔含(1996-),女,浙江天台人,硕士,助理畜牧师,研究方向为农产品质量安全及品质评价;杨晓君(1989-),男,上海人,本科,畜牧师,研究方向为农产品质量安全风险评估和营养品质评价。, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=g28HnX0dE2dsEAgo8C6+Nw==, pdfFileSize=3811509, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=上海市奶业优势特色产业集群科研类项目-都市奶业质量安全关键技术研究与集成应用(沪农委2024-74号))}, authors=null, keywords=[Keyword(id=1320326753650848159, tenantId=1146029695717560320, journalId=1296801528146919473, articleId=1320326752098955676, 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[10] 杜兵耀. 生乳中微生物风险关键点分析及假单胞菌特征研究[D].兰州:兰州大学,2022.)
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基于荟萃分析的乳及乳制品中假单胞菌属的生长动力学研究
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中国乳业 | 质量安全 2025,(6): 119-126
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中国乳业 |质量安全 2025 , (6) : 119 -126
基于荟萃分析的乳及乳制品中假单胞菌属的生长动力学研究
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江春铃1, 祁兵1, 朱华剑2, 马颖清1, 陈柔含1, 杨晓君1, 邓波1,*
作者信息
    1 上海市农产品质量安全中心, 上海 201708;
    2 上海理工大学, 上海 200093
通讯作者:
*邓波(1982-),男,湖北汉川人,硕士,高级兽医师,研究方向为农产品质量安全预测预警和风险评估。
作者简介:
江春铃(1998-),女,江苏无锡人,硕士,助理畜牧师,研究方向为食用农产品微生物检测;祁兵(1982-),男,江苏宿迁人,硕士,助理兽医师,研究方向为农产品质量安全预测预警和风险评估;朱华剑(1998-),男,江苏南通人,硕士,助理畜牧师,研究方向为食源性致病菌控制;马颖清(1984-),女,江苏射阳人,硕士,畜牧师,研究方向为农产品质量安全和品质;陈柔含(1996-),女,浙江天台人,硕士,助理畜牧师,研究方向为农产品质量安全及品质评价;杨晓君(1989-),男,上海人,本科,畜牧师,研究方向为农产品质量安全风险评估和营养品质评价。
Study on the Growth Kinetics of Pseudomonas spp. cryophilic bacteria in Raw Milk Based on Meta-analysis
  • JIANG Chunling1, QI Bing1, ZHU Huajian2, MA Yingqing1, CHEN Rouhan1, YANG Xiaojun1, DENG Bo1,*
  • Affiliations
      1 Shanghai Agricultural Products Quality and Safety Center, Shanghai 201708;
      2 University of Shanghai for Science and Technology, Shanghai 200093
    doi: 10.12377/1671-4393.25.06.20
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    假单胞菌属是生乳中最常见的嗜冷菌属之一,为探讨影响假单胞菌属动力学参数的主要因素,本研究以定量整合微生物动力学参数并量化菌株变异性的方法开展荟萃分析。通过统计750 余条假单胞菌属在不同条件下的生长参数,并基于平方根模型预测其最低生长温度(Tmin),结果表明,假单胞菌属整体Tmin为-4.06 ℃,其中菌株1248和E52在培养基中的Tmin分别为-5.38 ℃和-6.36 ℃。在低温环境下,UHT牛乳中高初始接菌量(Y0)显著缩短了迟滞期(λ),使微生物即使在较低温度下也能更快进入对数生长期。在温度(T)、pH值、水分活度(Aw)和NaCl浓度的共同作用下,最大比生长速率(μmax)分布范围为0.04~1.63 h-1。此外,NaCl浓度(1.0%~7.5%)对假单胞菌属的生长具有显著抑制作用。本研究可为乳及乳制品生产过程中的安全性、生产优化和质量控制提供重要参考依据。
    假单胞菌属  /  荟萃分析  /  生长  /  失活  /  建模
    Pseudomonas spp. is one of the most common genera of cryophilic bacteria in raw milk,and in order to explore the main factors affecting the kinetic parameters of Pseudomonas spp.,this study carried out a meta-analysis by quantitatively integrating the kinetic parameters of microorganisms and quantifying the variability of the strains.By statistically analyzing the growth parameters of more than 750 Pseudomonas spp. under different conditions and predicting their minimum growth temperatures(Tmin) based on the square root model,the results showed that the overall Tmin value of Pseudomonas spp. was -4.06 ℃,with the Tmin of strains 1248 and E52 in the culture medium being -5.38 ℃ and -6.36 ℃,respectively.In a low-temperature environment,the high initial inoculum size (Y0,log CFU/mL) in UHT milk significantly shortened the growth retardation period,allowing the microorganisms to enter the logarithmic growth period(λ) more quickly even at lower temperatures.The distribution of maximum specific growth rate(μmax) under the combined effect of temperature,pH value, activity water(Aw) and NaCl concentration ranged from 0.04 h-1 to 1.63 h-1.In addition,NaCl concentration(1.0% to 7.5%) had a significant inhibitory effect on the growth of Pseudomonas spp..This study provided important reference basis for the safety,production optimisation and quality control during the production of milk and dairy products.
    Pseudomonas spp.  /  meta-analysis  /  growth  /  inactivation  /  modelling
    江春铃, 祁兵, 朱华剑, 马颖清, 陈柔含, 杨晓君, 邓波. 基于荟萃分析的乳及乳制品中假单胞菌属的生长动力学研究. 中国乳业, 2025 , (6) : 119 -126 . DOI: 10.12377/1671-4393.25.06.20
    JIANG Chunling, QI Bing, ZHU Huajian, MA Yingqing, CHEN Rouhan, YANG Xiaojun, DENG Bo. Study on the Growth Kinetics of Pseudomonas spp. cryophilic bacteria in Raw Milk Based on Meta-analysis[J]. China Dairy, 2025 , (6) : 119 -126 . DOI: 10.12377/1671-4393.25.06.20

      上海市奶业优势特色产业集群科研类项目-都市奶业质量安全关键技术研究与集成应用(沪农委2024-74号)

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    2025年第卷第6期
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    doi: 10.12377/1671-4393.25.06.20
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    2种不同金属材料的力学参数
    科
    Family
    属数
    Number of
    genus
    种数
    Number of
    species
    占总种数比例
    Percentage of
    total species (%)
    属
    Genus
    种数
    Number of
    species
    占总种数比例
    Percentage of total
    species (%)
    鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
    小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
    多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
    红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
    小菇属 Mycena 11 5.26
    光柄菇属 Pluteus 5 2.39
    红菇属 Russula 17 8.13
    栓菌属 Trametes 5 2.39
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