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[Method] The average recovery rate was compared by comparing the relative standard deviation of extraction effects of primary water,anhydrous methanol and dichloromethane. [Result] When extracted with primary water,the relative standard deviation was 2.56% and the average recovery was 35.30%~51.12%. When extracted with anhydrous methanol,the relative standard deviation was 4.20%,and the average recovery was 81.32%~90.18%. When extracted with dichloromethane,the tissue status of the sample was abnormal,and the residue after nitrogen evaporation was oily,which could not be further determinated. [Conclusion] When extracted with anhydrous methanol. ELISA method had higher extraction accuracy and is more suitable for food determination., authors=WU Lan, HUANG Hua, DAI Xiaohong, FANG Juan, MA Houyang, WANG Dan, WU Jianxun, authorsList=WU Lan, HUANG Hua, DAI Xiaohong, FANG Juan, MA Houyang, WANG Dan, WU Jianxun, authorCompany=Mengniu Dairy Products Wuhan Co.,Ltd.,Wuhan Hubei 430040, correspAuthors=null, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1320063051370447516, articleId=1320063051156538011, tenantId=1146029695717560320, journalId=1296801528146919473, language=CN, title=提取剂对酶联免疫法检测芝士粉中黄曲霉毒素M₁的影响, columnId=1297134032135938673, journalTitle=中国乳业, columnName=质量安全, runingTitle=null, highlight=null, articleAbstract=[目的]本研究使用酶联免疫法（ELISA法）评估了一级水、无水甲醇、二氯甲烷3 种不同提取剂在检测芝士粉中黄曲霉毒素M₁含量方面的效果。[方法]通过对比一级水、无水甲醇、二氯甲烷3 种不同提取剂提取效果的相对标准偏差,比较平均回收率。[结果]用一级水提取时,相对标准偏差2.56%,平均回收率35.30%~51.12%;用无水甲醇提取时,相对标准偏差4.20%,平均回收率81.32%~90.18%;用二氯甲烷提取时,样品组织状态异常,氮流蒸发后残留物呈油状,无法进行后续检测。[结论]以无水甲醇作为提取剂时,ELISA法的提取准确性较高,更适用于食品检测领域。, authors=伍蓝, 黄华, 戴晓红, 方娟, 马后扬, 王丹, 吴建勋, authorsList=伍蓝, 黄华, 戴晓红, 方娟, 马后扬, 王丹, 吴建勋, authorCompany=蒙牛乳制品武汉有限责任公司,湖北武汉 430040, correspAuthors=null, authorNote=伍 蓝（1990-）,女,湖北安陆人,本科,研究方向为食品分析与检测;黄 华（1978-）,男,内蒙古呼和浩特人,硕士,研究方向为企业管理;戴晓红（1987-）,女,湖北黄冈人,本科,研究方向为食品安全;方 娟（1981-）,女,湖北武汉人,本科,研究方向为企业管理;马后扬（1986-）,男,湖北武汉人,本科,研究方向为企业管理;王 丹（1983-）,女,内蒙古呼和浩特人,硕士,副高级工程师,研究方向为企业管理;吴建勋（1981-）,男,内蒙古乌兰浩特人,本科,研究方向为企业管理。, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=VUJn4Nj/HD1tugspiRGh8g==, pdfFileSize=1970635, pdfExtLink=null, 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