Article(id=1319994006466056998, tenantId=1146029695717560320, journalId=1296801528146919473, issueId=1319993987860116227, articleNumber=null, orderNo=null, doi=10.12377/1671-4393.25.11.17, pmid=null, cstr=null, oa=null, hot=0, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=null, receivedDateStr=null, revisedDate=null, revisedDateStr=null, acceptedDate=null, acceptedDateStr=null, onlineDate=1792640695443, onlineDateStr=2026-10-22, pubDate=null, pubDateStr=null, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1792640695443, onlineIssueDateStr=2026-10-22, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1792640695443, creator=13701087609, updateTime=1792640695443, updator=13701087609, issue=Issue{id=1319993987860116227, tenantId=1146029695717560320, journalId=1296801528146919473, year='2025', volume='', issue='11', pageStart='1', pageEnd='166', issueExtLink='null', onlineDate='null', pubDate='1764000000000', pubDateStr='2025-11-25', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1792640691007, creator='13701087609', updateTime=1792641235550, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1319996271893524963, tenantId=1146029695717560320, journalId=1296801528146919473, issueId=1319993987860116227, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1319996271893524964, tenantId=1146029695717560320, journalId=1296801528146919473, issueId=1319993987860116227, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=137, endPage=146, ext={EN=ArticleExt(id=1319994006814184232, articleId=1319994006466056998, tenantId=1146029695717560320, journalId=1296801528146919473, language=EN, title=The Application of CHARM EPIC-PLUS Commercial Sterility Detection System in Microbial Detection of Milk Beverages, columnId=1297134031947194991, journalTitle=China Dairy, columnName=QUALITY CONTROL, runingTitle=null, highlight=null, articleAbstract=[Objective] The CHARM EPIC-PLUS commercial sterility detection system is a rapid microbial detection system based on the principle of adenosine triphosphate(ATP)bioluminescence. This system evaluates the microbial activity in samples by detecting the ATP content,achieving efficient determination of commercial sterility status. This study aims to verify the sensitivity and accuracy of the system in detection of milk beverages and improve the delivery time of the products.[Method] Bacillus subtilis,Escherichia coli and Saccharomyces cerevisiae were added to milk beverage. After incubation at(36±1)℃ for 48 hours,The CHARM EPIC-PLUS commercial sterility detection system and traditional national standard plate counting method were used for detection. After incubation at(36±1)℃ for 7 days simultaneously,the CHARM EPIC-PLUS commercial sterility detection system and the commercial sterility national standard method were used for detection, and the results were compared and analyzed.[Result] The results of the CHARM EPIC-PLUS commercial sterility detection system were highly consistent with the detection results of the commercial sterility national standard detection method. Moreover,compared with the 7-day detection cycle of the traditional commercial sterility national standard detection method,the CHARM EPIC-PLUS commercial sterility detection system significantly shortened the detection time to 48 hours.[Conclusion] The detection results of the CHARM EPIC-PLUS commercial sterility detection system were in good consistency with the national standard detection method of commercial sterility. It is an efficient,accurate detection method with broad application prospects., authors=WANG Lili, WANG Dan, XIE Xin, XING Yanxia, CAO Wenyan*, HOU Xiaofeng, ZHANG Nana, XU Ruixian, GENG Dandan, MIAO Jiajia, MA Jianjun, authorsList=WANG Lili, WANG Dan, XIE Xin, XING Yanxia, CAO Wenyan, HOU Xiaofeng, ZHANG Nana, XU Ruixian, GENG Dandan, MIAO Jiajia, MA Jianjun, authorCompany=Baoding Mengniu Beverage Co.,Ltd.,Hebei Province Key Laboratory of Intelligent Dairy Testing Technology,Baoding Hebei 072450, correspAuthors=null, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1319994006667383591, articleId=1319994006466056998, tenantId=1146029695717560320, journalId=1296801528146919473, language=CN, title=CHARM EPIC-PLUS商业无菌检测系统在乳饮料微生物检测中的应用, columnId=1297134032135938673, journalTitle=中国乳业, columnName=质量安全, runingTitle=null, highlight=null, articleAbstract=[目的]CHARM EPIC-PLUS商业无菌检测系统是一种基于三磷酸腺苷(ATP)生物发光原理的快速微生物检测系统,该系统通过检测ATP含量来评估样品中的微生物活性,实现对商业无菌状态的高效判定。本研究为验证该系统在乳饮料检测中的灵敏度与准确性,并提升产品的出货时效。[方法]添加枯草芽孢杆菌、大肠埃希氏菌、酿酒酵母至乳饮料,(36±1)℃培养48 h后,采用CHARM EPIC-PLUS商业无菌检测系统与传统国标平板计数法检测;同步(36±1)℃培养7 d后,采用CHARM EPIC-PLUS商业无菌检测系统与商业无菌国标方法检测,并对比分析结果。[结果]CHARM EPIC-PLUS商业无菌检测系统结果与传统国标平板计数法结果高度符合;该系统结果与商业无菌国标检测方法检测结果高度一致,且相较于传统商业无菌国标检测方法7 d的检测周期,CHARM EPIC-PLUS商业无菌检测系统显著缩短检测时间至48 h。[结论]CHARM EPIC-PLUS商业无菌检测系统的检测结果与商业无菌国标检测方法具有较好的一致性,是一种高效、准确,具有广阔应用前景的的检测方法。, authors=王丽丽, 王丹, 解鑫, 邢燕霞, 曹文燕*, 侯晓锋, 张娜娜, 徐瑞贤, 耿丹丹, 苗佳佳, 马建军, authorsList=王丽丽, 王丹, 解鑫, 邢燕霞, 曹文燕, 侯晓锋, 张娜娜, 徐瑞贤, 耿丹丹, 苗佳佳, 马建军, authorCompany=保定蒙牛饮料有限公司,河北省乳品智能化检测技术重点实验室,河北保定 072450, correspAuthors=*曹文燕(1987-),女,山东济南人,本科,副高级工程师,研究方向为食品质量与安全、食品检测和追溯系统。, authorNote=王丽丽(1991-),女,黑龙江绥化人,本科,中级工程师,研究方向为食品检验检测;王 丹(1983-),女,内蒙古呼和浩特人,硕士,副高级工程师,研究方向为发酵工程;解 鑫(1983-),男,内蒙古呼和浩特人,硕士,副高级工程师,研究方向为食品质量安全检验技术;邢燕霞(1988-),女,内蒙古乌兰察布人,本科,研究方向为食品质量与安全、食品检测和追溯系统;侯晓锋(1987-),男,河北张家口人,本科,研究方向为食品质量与安全、食品检测追溯系统;张娜娜(1993-),女,黑龙江绥化人,本科,中级工程师,研究方向为食品检验检测;徐瑞贤(1980-),女,河北邢台人,本科,中级工程师,研究方向为食品检验检测;耿丹丹(1988-),女,河北保定人,本科,中级工程师,研究方向为食品检验检测;苗佳佳(1987-),女,内蒙古乌兰察布人,本科,中级工程师,研究方向为食品检验检测;马建军(1984-),男,内蒙古呼和浩特人,本科,研究方向为行政管理。, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=DfHE1l7e3SRKIm+cj9dfCg==, pdfFileSize=2041326, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=河北省乳制品智能化检测技术重点实验室项目(SZQ2020018))}, authors=[Author(id=1324196060964467481, tenantId=1146029695717560320, journalId=null, articleId=1319994006466056998, orderNo=null, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=null, email=null, emailSecond=null, emailThird=null, correspondingAuthor=null, authorType=null, ext={EN=AuthorExt(id=null, tenantId=null, journalId=1296801528146919473, articleId=1319994006466056998, authorId=1324196060964467481, language=EN, stringName=WANG Lili, WANG Dan, XIE Xin, XING Yanxia, CAO Wenyan, HOU Xiaofeng, ZHANG Nana, XU Ruixian, GENG Dandan, MIAO Jiajia, MA Jianjun, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=null, address=null, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=null, tenantId=null, journalId=1296801528146919473, articleId=1319994006466056998, authorId=1324196060964467481, language=CN, stringName=王丽丽, 王丹, 解鑫, 邢燕霞, 曹文燕, 侯晓锋, 张娜娜, 徐瑞贤, 耿丹丹, 苗佳佳, 马建军, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=null, address=null, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=null)], keywords=[Keyword(id=1319994006935819049, tenantId=1146029695717560320, journalId=1296801528146919473, articleId=1319994006466056998, language=CN, orderNo=1, keyword=三磷酸腺苷(ATP)生物荧光), Keyword(id=1319994007019705130, tenantId=1146029695717560320, journalId=1296801528146919473, articleId=1319994006466056998, language=CN, orderNo=2, keyword=商业无菌), Keyword(id=1319994007116174123, tenantId=1146029695717560320, journalId=1296801528146919473, articleId=1319994006466056998, language=CN, orderNo=3, keyword=快速检测技术), Keyword(id=1319994007233614636, tenantId=1146029695717560320, journalId=1296801528146919473, articleId=1319994006466056998, language=EN, orderNo=1, keyword=adenosine triphosphate(ATP)bioluminescence), Keyword(id=1319994007300723501, tenantId=1146029695717560320, journalId=1296801528146919473, articleId=1319994006466056998, language=EN, orderNo=2, keyword=commercial sterility), Keyword(id=1319994007372026670, tenantId=1146029695717560320, journalId=1296801528146919473, articleId=1319994006466056998, language=EN, orderNo=3, keyword=rapid detection technology)], refs=null, funds=null, companyList=null, figs=null, attaches=null, journal=Journal(id=1296801172792901679, delFlag=0, nameCn=中国乳业, nameEn=China Dairy, nameHistory1=null, nameHistory2=null, issn=1671-4393, eissn=null, cn=11-4768/S, coden=null, periodic=0, language=CN, oaType=null, ccby=null, superviseOffice=null, ownerOffice=null, pubOffice=null, editorOffice=null, officeType=null, aims=null, clcCode=null, officeProv=null, officeCity=null, officeAddr=null, officeZip=null, officeEmail=null, officePhone=null, editDirector=null, officeDirector=null, officeDirectorPhone=null, officeStaffNum=null, officeEmpNum=null, coverPicUrl=fy0U6rTUhrcBdKszdYVe3w==, journalPrice=null, startedYear=null, abbrevIsoEn=China Dairy, journalRemark=null, publicationField=null, createdTime=1787111092997, updatedTime=1788949480572, createdBy=18614031015, updatedBy=13041195026, firstLetterCn=Z, firstLetterEn=Z, subjectCode=Agriculture, subjectName=null, subjectCodeEn=Agriculture, subjectNameEn=null, picCn=fy0U6rTUhrcBdKszdYVe3w==, picEn=1lm7RNSd456gWkkoaDGdEQ==, jcr=null, cjcr=null, exts=[JournalExt(id=1304511929255481461, language=CN, name=中国乳业, nameHistory1=null, nameHistory2=null, managedBy=, sponsoredBy=, publishedBy=, editorOffice=, officeProv=null, officeCity=null, officeAddr=, officeZip=, editDirector=, officeDirector=null, officePhone=null, coverPicUrl=null, journalRemark=, submitArticleUrl=null, websiteUrl=, createdTime=1788949480594, updatedTime=1788949480594, createdBy=13041195026, updatedBy=13041195026, submissionGuidelinesUrl=, submissionAuthorUrl=https://zgryauthor.cast.org.cn/webm, submissionEditorUrl=https://zgryeditor.cast.org.cn/webm, submissionReviewUrl=https://zgryauthor.cast.org.cn/webm, submissionCeEditorUrl=, submissionAeEditorUrl=, option={"copyright":""}), JournalExt(id=1304511929301618806, language=EN, name=China Dairy, nameHistory1=null, nameHistory2=null, managedBy=, sponsoredBy=, publishedBy=, editorOffice=, officeProv=null, officeCity=null, officeAddr=, officeZip=, editDirector=, officeDirector=null, officePhone=null, coverPicUrl=null, journalRemark=, submitArticleUrl=null, websiteUrl=, createdTime=1788949480605, updatedTime=1788949480605, createdBy=13041195026, updatedBy=13041195026, submissionGuidelinesUrl=, submissionAuthorUrl=https://zgryauthor.cast.org.cn/webm, submissionEditorUrl=https://zgryeditor.cast.org.cn/webm, submissionReviewUrl=https://zgryauthor.cast.org.cn/webm, submissionCeEditorUrl=, submissionAeEditorUrl=, option={"copyright":""})], databaseList=null, tenantJournalId=1296801528146919473, websiteList=[Website(id=1296802542923572121, webName=null, webTitle=null, webDomain=null, webCopyrigh=null, webIpcNo=null, seoTitle=null, seoKeywords=null, seoDescription=null, tenantJournalId=null, journalId=1296801528146919473, journalNameCn=null, journalNameEn=null, grayFlag=null, tenantId=1146029695717560320, platformId=null, journalGroupId=null, journalGroupNameCn=null, journalGroupNameEn=null, type=1, domain=https://castjournals.cast.org.cn/joweb/zgry/CN, language=CN, createTime=1787111419659, createBy=18614031015, updateTime=1787111471367, updateBy=18614031015, name=中国乳业-中文, tplId=1146099689490845704, title=中国乳业, delFlag=0, indexPage=/home, props=[WebsiteProps(id=1296802881777198042, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1296802542923572121, code=articleTextType, value=kx, createTime=1787111500448, updateTime=1787111500448, creator=18614031015, updator=18614031015), WebsiteProps(id=1296802881747837911, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1296802542923572121, code=banner, value=null, createTime=1787111500441, updateTime=1787111500441, creator=18614031015, updator=18614031015), WebsiteProps(id=1296802881827529693, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1296802542923572121, code=grayFlag, value=0, createTime=1787111500460, updateTime=1787111500460, creator=18614031015, updator=18614031015), WebsiteProps(id=1296802881735254998, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1296802542923572121, code=logo, value=https://castjournals.cast.org.cn/joweb/zgry/CN/file/pic?fileId=qVbzVKBQlSo4CZ2uDrqTZw==, createTime=1787111500438, updateTime=1787111500438, creator=18614031015, updator=18614031015), WebsiteProps(id=1296802881844306911, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1296802542923572121, code=minRunFlag, value=0, createTime=1787111500464, updateTime=1787111500464, creator=18614031015, updator=18614031015), WebsiteProps(id=1296802881768809433, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1296802542923572121, code=picServerUrl, value=https://castjournals.cast.org.cn/joweb/zgry/CN/file/pic, createTime=1787111500446, updateTime=1787111500446, creator=18614031015, updator=18614031015), WebsiteProps(id=1296802881835918302, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1296802542923572121, code=silenceFlag, value=0, createTime=1787111500462, updateTime=1787111500462, creator=18614031015, updator=18614031015), WebsiteProps(id=1296802881760420824, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1296802542923572121, code=staticResourcePath, value=https://castjournals.cast.org.cn/joweb/cast_kjdb_cn_619/, createTime=1787111500444, updateTime=1787111500444, creator=18614031015, updator=18614031015), WebsiteProps(id=1296802881810752475, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1296802542923572121, code=themeColor, value=null, createTime=1787111500456, updateTime=1787111500456, creator=18614031015, updator=18614031015), WebsiteProps(id=1296802881819141084, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1296802542923572121, code=themeStyle, value=null, createTime=1787111500458, updateTime=1787111500458, creator=18614031015, updator=18614031015)]), Website(id=1296802543032624028, webName=null, webTitle=null, webDomain=null, webCopyrigh=null, webIpcNo=null, seoTitle=null, seoKeywords=null, seoDescription=null, tenantJournalId=null, journalId=1296801528146919473, journalNameCn=null, journalNameEn=null, grayFlag=null, tenantId=1146029695717560320, platformId=null, journalGroupId=null, journalGroupNameCn=null, journalGroupNameEn=null, type=1, domain=https://castjournals.cast.org.cn/joweb/zgry/EN, language=EN, createTime=1787111419685, createBy=18614031015, updateTime=1787111462939, updateBy=18614031015, name=中国乳业-英文, tplId=1146101810881728533, title=China Dairy, delFlag=0, indexPage=/home, props=[WebsiteProps(id=1296802850798068681, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1296802543032624028, code=articleTextType, value=kx, createTime=1787111493062, updateTime=1787111493062, creator=18614031015, updator=18614031015), WebsiteProps(id=1296802850768708550, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1296802543032624028, code=banner, value=null, createTime=1787111493055, updateTime=1787111493055, creator=18614031015, updator=18614031015), WebsiteProps(id=1296802850819040204, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1296802543032624028, code=grayFlag, value=0, createTime=1787111493067, updateTime=1787111493067, creator=18614031015, updator=18614031015), WebsiteProps(id=1296802850760319941, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1296802543032624028, code=logo, value=https://castjournals.cast.org.cn/joweb/zgry/EN/file/pic?fileId=qVbzVKBQlSo4CZ2uDrqTZw==, createTime=1787111493053, updateTime=1787111493053, creator=18614031015, updator=18614031015), WebsiteProps(id=1296802850831623118, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1296802543032624028, code=minRunFlag, value=0, createTime=1787111493070, updateTime=1787111493070, creator=18614031015, updator=18614031015), WebsiteProps(id=1296802850789680072, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1296802543032624028, code=picServerUrl, value=https://castjournals.cast.org.cn/joweb/zgry/EN/file/pic, createTime=1787111493060, updateTime=1787111493060, creator=18614031015, updator=18614031015), WebsiteProps(id=1296802850823234509, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1296802543032624028, code=silenceFlag, value=0, createTime=1787111493068, updateTime=1787111493068, creator=18614031015, updator=18614031015), WebsiteProps(id=1296802850781291463, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1296802543032624028, code=staticResourcePath, value=https://castjournals.cast.org.cn/joweb/cast_kjdb_en_623/, createTime=1787111493058, updateTime=1787111493058, creator=18614031015, updator=18614031015), WebsiteProps(id=1296802850802262986, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1296802543032624028, code=themeColor, value=null, createTime=1787111493063, updateTime=1787111493063, creator=18614031015, updator=18614031015), WebsiteProps(id=1296802850810651595, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1296802543032624028, code=themeStyle, value=null, createTime=1787111493065, updateTime=1787111493065, creator=18614031015, updator=18614031015)])], journalTitle=中国乳业, weixinUrl=null, journalUrl=http://zhgry.aiijournal.com/, iacademicId=null, status=1, seqNo=null, journalTitleEn=China Dairy, journalPhotoCn=fy0U6rTUhrcBdKszdYVe3w==, journalPhotoEn=1lm7RNSd456gWkkoaDGdEQ==, journalFirstLetter=Z, journalRecommend=null, journalNew=null, journalCollection=null, jcrJf=null, cjcrJf=null, jcrJfStr=null, cjcrJfStr=null, submissionFirstDecision=null, sciSubjectClassification=null, casSubjectClassification=null, citeScore=null, totalCitationFrequency=null, icpCode=null, psCode=null, advertisingLicenseCode=null, copyrightInformation=null, country=null, option=, provinceCode=null, provinceName=null, collectFlag=false, interPubPlatform=, interPubPlatformUrl=null), detailUrlCn=https://castjournals.cast.org.cn/joweb/zgry/CN/10.12377/1671-4393.25.11.17, detailUrlEn=https://castjournals.cast.org.cn/joweb/zgry/EN/10.12377/1671-4393.25.11.17, pdfUrlCn=https://castjournals.cast.org.cn/joweb/zgry/CN/PDF/10.12377/1671-4393.25.11.17, pdfUrlEn=https://castjournals.cast.org.cn/joweb/zgry/EN/PDF/10.12377/1671-4393.25.11.17, aliStartDate=null, aliEndDate=null, collectionFlag=false, citedCount=null, citedUrl=null, previewStatus=0, delFlag=0, hasFullText=0, orderTime=1792640695443, fullTextJson=null, articleText=null, reference=[1] 陈雅敏. 浅析食品快速检测技术在基层食品安全监管中的运用[J].现代食品,2023,29(18):136-138.
[2] 陈兆鑫,梁一帆,林文锋,等.快速检测技术在食品安全监管中的应用分析[J].中国食品,2024(20):95-97.
[3] 中华人民共和国国家卫生健康委员会,国家市场监督管理总局. GB4789.26—2023,食品安全国家标准食品微生物学检验商业无菌检验[S].
[4] 21 CFR Part 113 -2011.Thermally processed low-acid foods packaged in hermetically sealed containers.
[5] 东思源,仇凯,孟镇,等.食品商业无菌国内外标准法规对比研究及标准化工作思考[J].食品与发酵工业,2022,48(22):305-310.
[6] 涂宝峰. 食品微生物检验中罐头食品商业无菌检验探讨[J].科技创新与生产力,2019(7):38-39.
[7] 中华人民共和国国家卫生健康委员会,国家市场监督管理总局.GB7101-2022 食品安全国家标准饮料[S].
[8] 梁好. 食品快检技术在食品检测中的应用[J].食品安全导刊,2024(9):161-163.
[9] 吴慧清,吴清平,张菊梅,等.生物发光检测法和国标法检测样品中活菌总数的比较研究[J].食品工业科技,2004(4):134-135,85
[10] 毛映丹. ATP生物发光法检测水中细菌数的研究[D].上海:华东师范大学,2009.
[11] 张桐,谢茂梅,刘艺丹,等.三磷酸腺苷生物发光法在提高复杂样品细菌检测能力方面的应用研究进展[J].食品安全质量检测学报,2024,15(14):1-8.
[12] 林玲,陈惠,郭广生.三磷酸腺苷生物发光法快速测定食品中细菌的含量[J].分析试验室,2009,28(7):26-30.
[13] 中华人民共和国国家卫生健康委员会,国家市场监督管理总局. GB4789.2—2022,食品安全国家标准食品微生物学检验菌落总数测定[S].
[14] 中华人民共和国国家卫生和计划生育委员会,国家食品药品监督理蒽局. GB4789.3—2016 食品安全国家标准食品微生物学检验大肠菌群计数[S].
[15] 刘龙海,李新圃,杨峰,等.酿酒酵母菌生长特性的研究[J].中国草食动物科学,2016,36(3):38-41.
[16] 中华人民共和国卫生部. GB4789.15—2010 食品安全国家标准食品微生物学检验霉苗和酵母计数[S].
[17] 马静,骆敏,宋艳梅,等.酸奶及乳饮料中大肠杆菌变化趋势研究[J].中国乳业,2023(1):64-69.
[18] 刘娜,王亚萍,李景云,等.基于流式细胞仪提高乳制品商业无菌检验时效性的研究[J].食品安全质量检测学报,2024,15(20):131-139.)
收藏切换
CHARM EPIC-PLUS商业无菌检测系统在乳饮料微生物检测中的应用
收藏切换
PDF下载
王丽丽, 王丹, 解鑫, 邢燕霞, 曹文燕, 侯晓锋, 张娜娜, 徐瑞贤, 耿丹丹, 苗佳佳, 马建军
中国乳业 | 质量安全 2025,(11): 137-146
收起
收藏切换
中国乳业 |质量安全 2025 , (11) : 137 -146
CHARM EPIC-PLUS商业无菌检测系统在乳饮料微生物检测中的应用
全屏
[Author(id=1324196060964467481, tenantId=1146029695717560320, journalId=null, articleId=1319994006466056998, orderNo=null, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=null, email=null, emailSecond=null, emailThird=null, correspondingAuthor=null, authorType=null, ext={EN=AuthorExt(id=null, tenantId=null, journalId=1296801528146919473, articleId=1319994006466056998, authorId=1324196060964467481, language=EN, stringName=WANG Lili, WANG Dan, XIE Xin, XING Yanxia, CAO Wenyan, HOU Xiaofeng, ZHANG Nana, XU Ruixian, GENG Dandan, MIAO Jiajia, MA Jianjun, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=null, address=null, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=null, tenantId=null, journalId=1296801528146919473, articleId=1319994006466056998, authorId=1324196060964467481, language=CN, stringName=王丽丽, 王丹, 解鑫, 邢燕霞, 曹文燕, 侯晓锋, 张娜娜, 徐瑞贤, 耿丹丹, 苗佳佳, 马建军, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=null, address=null, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=null)]
王丽丽, 王丹, 解鑫, 邢燕霞, 曹文燕, 侯晓锋, 张娜娜, 徐瑞贤, 耿丹丹, 苗佳佳, 马建军
作者信息
通讯作者:
*曹文燕(1987-),女,山东济南人,本科,副高级工程师,研究方向为食品质量与安全、食品检测和追溯系统。
作者简介:
王丽丽(1991-),女,黑龙江绥化人,本科,中级工程师,研究方向为食品检验检测;王 丹(1983-),女,内蒙古呼和浩特人,硕士,副高级工程师,研究方向为发酵工程;解 鑫(1983-),男,内蒙古呼和浩特人,硕士,副高级工程师,研究方向为食品质量安全检验技术;邢燕霞(1988-),女,内蒙古乌兰察布人,本科,研究方向为食品质量与安全、食品检测和追溯系统;侯晓锋(1987-),男,河北张家口人,本科,研究方向为食品质量与安全、食品检测追溯系统;张娜娜(1993-),女,黑龙江绥化人,本科,中级工程师,研究方向为食品检验检测;徐瑞贤(1980-),女,河北邢台人,本科,中级工程师,研究方向为食品检验检测;耿丹丹(1988-),女,河北保定人,本科,中级工程师,研究方向为食品检验检测;苗佳佳(1987-),女,内蒙古乌兰察布人,本科,中级工程师,研究方向为食品检验检测;马建军(1984-),男,内蒙古呼和浩特人,本科,研究方向为行政管理。
The Application of CHARM EPIC-PLUS Commercial Sterility Detection System in Microbial Detection of Milk Beverages
WANG Lili, WANG Dan, XIE Xin, XING Yanxia, CAO Wenyan, HOU Xiaofeng, ZHANG Nana, XU Ruixian, GENG Dandan, MIAO Jiajia, MA Jianjun
Affiliations
    Baoding Mengniu Beverage Co.,Ltd.,Hebei Province Key Laboratory of Intelligent Dairy Testing Technology,Baoding Hebei 072450
doi: 10.12377/1671-4393.25.11.17
文章导航
收藏切换
[目的]CHARM EPIC-PLUS商业无菌检测系统是一种基于三磷酸腺苷(ATP)生物发光原理的快速微生物检测系统,该系统通过检测ATP含量来评估样品中的微生物活性,实现对商业无菌状态的高效判定。本研究为验证该系统在乳饮料检测中的灵敏度与准确性,并提升产品的出货时效。[方法]添加枯草芽孢杆菌、大肠埃希氏菌、酿酒酵母至乳饮料,(36±1)℃培养48 h后,采用CHARM EPIC-PLUS商业无菌检测系统与传统国标平板计数法检测;同步(36±1)℃培养7 d后,采用CHARM EPIC-PLUS商业无菌检测系统与商业无菌国标方法检测,并对比分析结果。[结果]CHARM EPIC-PLUS商业无菌检测系统结果与传统国标平板计数法结果高度符合;该系统结果与商业无菌国标检测方法检测结果高度一致,且相较于传统商业无菌国标检测方法7 d的检测周期,CHARM EPIC-PLUS商业无菌检测系统显著缩短检测时间至48 h。[结论]CHARM EPIC-PLUS商业无菌检测系统的检测结果与商业无菌国标检测方法具有较好的一致性,是一种高效、准确,具有广阔应用前景的的检测方法。
三磷酸腺苷(ATP)生物荧光  /  商业无菌  /  快速检测技术
[Objective] The CHARM EPIC-PLUS commercial sterility detection system is a rapid microbial detection system based on the principle of adenosine triphosphate(ATP)bioluminescence. This system evaluates the microbial activity in samples by detecting the ATP content,achieving efficient determination of commercial sterility status. This study aims to verify the sensitivity and accuracy of the system in detection of milk beverages and improve the delivery time of the products.[Method] Bacillus subtilis,Escherichia coli and Saccharomyces cerevisiae were added to milk beverage. After incubation at(36±1)℃ for 48 hours,The CHARM EPIC-PLUS commercial sterility detection system and traditional national standard plate counting method were used for detection. After incubation at(36±1)℃ for 7 days simultaneously,the CHARM EPIC-PLUS commercial sterility detection system and the commercial sterility national standard method were used for detection, and the results were compared and analyzed.[Result] The results of the CHARM EPIC-PLUS commercial sterility detection system were highly consistent with the detection results of the commercial sterility national standard detection method. Moreover,compared with the 7-day detection cycle of the traditional commercial sterility national standard detection method,the CHARM EPIC-PLUS commercial sterility detection system significantly shortened the detection time to 48 hours.[Conclusion] The detection results of the CHARM EPIC-PLUS commercial sterility detection system were in good consistency with the national standard detection method of commercial sterility. It is an efficient,accurate detection method with broad application prospects.
adenosine triphosphate(ATP)bioluminescence  /  commercial sterility  /  rapid detection technology
王丽丽, 王丹, 解鑫, 邢燕霞, 曹文燕, 侯晓锋, 张娜娜, 徐瑞贤, 耿丹丹, 苗佳佳, 马建军. CHARM EPIC-PLUS商业无菌检测系统在乳饮料微生物检测中的应用. 中国乳业, 2025 , (11) : 137 -146 . DOI: 10.12377/1671-4393.25.11.17
WANG Lili, WANG Dan, XIE Xin, XING Yanxia, CAO Wenyan, HOU Xiaofeng, ZHANG Nana, XU Ruixian, GENG Dandan, MIAO Jiajia, MA Jianjun. The Application of CHARM EPIC-PLUS Commercial Sterility Detection System in Microbial Detection of Milk Beverages[J]. China Dairy, 2025 , (11) : 137 -146 . DOI: 10.12377/1671-4393.25.11.17

    河北省乳制品智能化检测技术重点实验室项目(SZQ2020018)

参考文献 引证文献
排序方式:
[1] 陈雅敏. 浅析食品快速检测技术在基层食品安全监管中的运用[J].现代食品,2023,29(18):136-138.
[2] 陈兆鑫,梁一帆,林文锋,等.快速检测技术在食品安全监管中的应用分析[J].中国食品,2024(20):95-97.
[3] 中华人民共和国国家卫生健康委员会,国家市场监督管理总局. GB4789.26—2023,食品安全国家标准食品微生物学检验商业无菌检验[S].
[4] 21 CFR Part 113 -2011.Thermally processed low-acid foods packaged in hermetically sealed containers.
[5] 东思源,仇凯,孟镇,等.食品商业无菌国内外标准法规对比研究及标准化工作思考[J].食品与发酵工业,2022,48(22):305-310.
[6] 涂宝峰. 食品微生物检验中罐头食品商业无菌检验探讨[J].科技创新与生产力,2019(7):38-39.
[7] 中华人民共和国国家卫生健康委员会,国家市场监督管理总局.GB7101-2022 食品安全国家标准饮料[S].
[8] 梁好. 食品快检技术在食品检测中的应用[J].食品安全导刊,2024(9):161-163.
[9] 吴慧清,吴清平,张菊梅,等.生物发光检测法和国标法检测样品中活菌总数的比较研究[J].食品工业科技,2004(4):134-135,85
[10] 毛映丹. ATP生物发光法检测水中细菌数的研究[D].上海:华东师范大学,2009.
[11] 张桐,谢茂梅,刘艺丹,等.三磷酸腺苷生物发光法在提高复杂样品细菌检测能力方面的应用研究进展[J].食品安全质量检测学报,2024,15(14):1-8.
[12] 林玲,陈惠,郭广生.三磷酸腺苷生物发光法快速测定食品中细菌的含量[J].分析试验室,2009,28(7):26-30.
[13] 中华人民共和国国家卫生健康委员会,国家市场监督管理总局. GB4789.2—2022,食品安全国家标准食品微生物学检验菌落总数测定[S].
[14] 中华人民共和国国家卫生和计划生育委员会,国家食品药品监督理蒽局. GB4789.3—2016 食品安全国家标准食品微生物学检验大肠菌群计数[S].
[15] 刘龙海,李新圃,杨峰,等.酿酒酵母菌生长特性的研究[J].中国草食动物科学,2016,36(3):38-41.
[16] 中华人民共和国卫生部. GB4789.15—2010 食品安全国家标准食品微生物学检验霉苗和酵母计数[S].
[17] 马静,骆敏,宋艳梅,等.酸奶及乳饮料中大肠杆菌变化趋势研究[J].中国乳业,2023(1):64-69.
[18] 刘娜,王亚萍,李景云,等.基于流式细胞仪提高乳制品商业无菌检验时效性的研究[J].食品安全质量检测学报,2024,15(20):131-139.
2025年第卷第11期
PDF下载
71
10
引用本文
BibTeX
文章信息
doi: 10.12377/1671-4393.25.11.17
  • 首发时间:2026-10-22
补充材料
相关文章
文章信息
作者
出版历史
基金
作者信息
参考文献
分享链接
https://castjournals.cast.org.cn/joweb/zgry/CN/10.12377/1671-4393.25.11.17
分享至
全文二维码

扫描看全文

引用本文
BibTeX
本文的引用情况
2种不同金属材料的力学参数
科
Family
属数
Number of
genus
种数
Number of
species
占总种数比例
Percentage of
total species (%)
属
Genus
种数
Number of
species
占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
关闭全屏