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Based on the results of discontinuous pH measurement,the four parameters were identified by using nonlinear least square method,and then the acidification activity was determined by bisection method. The optimization was verified based on results of continuous pH measurement.[Result] The determination coefficient(R2)was 0.9988,root-mean-square error(RMSE)was 0.03,which showed that the model gave good fits of the data. The model decreased the relative error of discontinuous pH measurement from 10.62% to 2.21%.[Conclusion] The model can improved the accuracy of discontinuous pH measurement,however,the modified Gompertz model had limitations in providing other fermentation kinetic parameters due to the complicated biochemistry reation in actual fermentation process., authors=BAI Xiaotong, CHEN Chunyu, CHEN Beilei, HUANG Junguang, YANG Aijun*, authorsList=BAI Xiaotong, CHEN Chunyu, CHEN Beilei, HUANG Junguang, YANG Aijun, authorCompany=Guangdong Yantang Dairy Co.,Ltd.,Guangzhou Guangdong 511356, correspAuthors=null, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, 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totalCitationFrequency=null, icpCode=null, psCode=null, advertisingLicenseCode=null, copyrightInformation=null, country=null, option=, provinceCode=null, provinceName=null, collectFlag=false, interPubPlatform=, interPubPlatformUrl=null), detailUrlCn=https://castjournals.cast.org.cn/joweb/zgry/CN/10.12377/1671-4393.25.11.15, detailUrlEn=https://castjournals.cast.org.cn/joweb/zgry/EN/10.12377/1671-4393.25.11.15, pdfUrlCn=https://castjournals.cast.org.cn/joweb/zgry/CN/PDF/10.12377/1671-4393.25.11.15, pdfUrlEn=https://castjournals.cast.org.cn/joweb/zgry/EN/PDF/10.12377/1671-4393.25.11.15, aliStartDate=null, aliEndDate=null, collectionFlag=false, citedCount=null, citedUrl=null, previewStatus=0, delFlag=0, hasFullText=0, orderTime=1792640693159, fullTextJson=null, articleText=null, reference=[1] De Brabandere A G,De Baerdemaeker J G. 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[3] Shen X,Li W,Cai H,et al.Metabolomics analysis reveals differences in milk metabolism and fermentation rate between individual Lactococcus lactis subsp. lactis strains[J]. Food research international,2022,162(Pt A):111920.
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基于修正Gompertz模型的非连续pH值测量法对乳酸菌产酸活力检测的优化研究
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中国乳业 | 质量安全 2025,(11): 125-129
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中国乳业 |质量安全 2025 , (11) : 125 -129
基于修正Gompertz模型的非连续pH值测量法对乳酸菌产酸活力检测的优化研究
全屏
白晓彤, 陈春裕, 陈蓓蕾, 黄隽光, 杨爱君*
作者信息
    广东燕塘乳业股份有限公司,广东广州 511356
通讯作者:
*杨爱君(1970-),女,广东潮州人,硕士,正高级工程师,研究方向为乳制品质量检测和质量保证。
作者简介:
白晓彤(1998-),女,广东广州人,硕士,研究方向为供应链与原材料质量控制;陈春裕(1994-),女,广东韶关人,本科,工程师,研究方向为供应链与原材料质量控制;陈蓓蕾(1998-),女,湖北武汉人,本科,助理工程师,研究方向为供应链与原材料质量控制;黄隽光(1999-),男,广东佛山人,本科,助理工程师,研究方向为供应链与原材料质量控制。
Optimization Research on the Detection of Acidification Activity of Lactic Acid Bacteria by Discontinuous pH Measurement Based on Modified Gompertz Model
  • BAI Xiaotong, CHEN Chunyu, CHEN Beilei, HUANG Junguang, YANG Aijun*
  • Affiliations
      Guangdong Yantang Dairy Co.,Ltd.,Guangzhou Guangdong 511356
    doi: 10.12377/1671-4393.25.11.15
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    [目的]通过修正Gompertz模型拟合的方法提高非连续法测量产酸活力的准确性。[方法]该模型的运行基于4 个发酵动力学参数:停滞期(λ)、发酵最大酸化速率(μ)、初始pH值(pH0)和终点pH值(pH∞)。基于非连续测量法结果,使用非线性最小二乘法辨别出模型中4 个发酵动力学参数,通过二分法数值求解得到产酸活力结果。优化效果基于连续测量的结果进行验证。[结果]拟合曲线的决定系数(R2)为0.9988,均方根误差(RMSE)为0.03,该模型拟合效果良好。该模型使得非连续测量法的测量误差由10.62%缩小至2.21%。[结论]该模型能有效提高非连续测量产酸活力的准确性,但由于实际发酵过程中复杂的生化反应,该模型在提供其他发酵动力学参数方面存在局限性。
    乳酸菌  /  产酸活力  /  Gompertz模型  /  非连续pH值测量
    [Objective] This study aimed at improving the accuracy of the discontinuous pH measurement in the determination of acidification activity by modeling fitting based on the modified Gompertz model.[Method] The model operated based on four key parameters:the lag phase time(λ),maximal rate of pH decrease with time(μ),initial pH(pH0),and final pH(pH∞). Based on the results of discontinuous pH measurement,the four parameters were identified by using nonlinear least square method,and then the acidification activity was determined by bisection method. The optimization was verified based on results of continuous pH measurement.[Result] The determination coefficient(R2)was 0.9988,root-mean-square error(RMSE)was 0.03,which showed that the model gave good fits of the data. The model decreased the relative error of discontinuous pH measurement from 10.62% to 2.21%.[Conclusion] The model can improved the accuracy of discontinuous pH measurement,however,the modified Gompertz model had limitations in providing other fermentation kinetic parameters due to the complicated biochemistry reation in actual fermentation process.
    lactic acid bacteria  /  acidification activity  /  Gompertz model  /  discontinuous pH measurement
    白晓彤, 陈春裕, 陈蓓蕾, 黄隽光, 杨爱君. 基于修正Gompertz模型的非连续pH值测量法对乳酸菌产酸活力检测的优化研究. 中国乳业, 2025 , (11) : 125 -129 . DOI: 10.12377/1671-4393.25.11.15
    BAI Xiaotong, CHEN Chunyu, CHEN Beilei, HUANG Junguang, YANG Aijun. Optimization Research on the Detection of Acidification Activity of Lactic Acid Bacteria by Discontinuous pH Measurement Based on Modified Gompertz Model[J]. China Dairy, 2025 , (11) : 125 -129 . DOI: 10.12377/1671-4393.25.11.15

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    2025年第卷第11期
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    doi: 10.12377/1671-4393.25.11.15
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    2种不同金属材料的力学参数
    科
    Family
    属数
    Number of
    genus
    种数
    Number of
    species
    占总种数比例
    Percentage of
    total species (%)
    属
    Genus
    种数
    Number of
    species
    占总种数比例
    Percentage of total
    species (%)
    鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
    小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
    多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
    红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
    小菇属 Mycena 11 5.26
    光柄菇属 Pluteus 5 2.39
    红菇属 Russula 17 8.13
    栓菌属 Trametes 5 2.39
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