Article(id=1297134055384961905, tenantId=1146029695717560320, journalId=1296801528146919473, issueId=1297134028650468001, articleNumber=null, orderNo=null, doi=10.12377/1671-4393.26.03.11, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=null, receivedDateStr=null, revisedDate=null, revisedDateStr=null, acceptedDate=null, acceptedDateStr=null, onlineDate=1787190458388, onlineDateStr=2026-08-20, pubDate=1774368000000, pubDateStr=2026-03-25, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1787190458388, onlineIssueDateStr=2026-08-20, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1787190458388, creator=13701087609, updateTime=1787190458388, updator=13701087609, issue=Issue{id=1297134028650468001, tenantId=1146029695717560320, journalId=1296801528146919473, year='2026', volume='', issue='3', pageStart='2', pageEnd='157', issueExtLink='null', onlineDate='null', pubDate='1774368000000', pubDateStr='2026-03-25', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1787190452015, creator='13701087609', updateTime=1787192267815, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1297141644734787890, tenantId=1146029695717560320, journalId=1296801528146919473, issueId=1297134028650468001, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1297141644734787891, tenantId=1146029695717560320, journalId=1296801528146919473, issueId=1297134028650468001, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=73, endPage=79, ext={EN=ArticleExt(id=1297134055577899890, articleId=1297134055384961905, tenantId=1146029695717560320, journalId=1296801528146919473, language=EN, title=Research Progress on Bioengineering Detection Methods of Thermoduric Proteinases in Dairy Products, columnId=1297134031947194991, journalTitle=China Dairy, columnName=QUALITY CONTROL, runingTitle=null, highlight=null, articleAbstract=

Thermoduric proteinases are a critical risk factor affecting the quality stability of dairy products. Their thermoduric property makes them difficult to achieve complete inactivation through conventional sterilization processes, thereby leading to protein hydrolysis and sensory quality deterioration of the product during its shelf life. This paper systematically analyzed the microbial sources of thermoduric proteinases (e.g., Pseudomonas, Bacillus) and their thermal stability mechanisms (e.g., hydrophobic interactions, metal ion dependence), and focused on the innovative progress of detection technologies and the bottlenecks encountered in industrial application. Among current detection methods, the colorimetric assays are cost-effective but lack sensitivity, while fluorescence-based techniques (e.g., FRET) offer high sensitivity but are limited by substrate universality. Moreover, the molecular biology (PCR) and immunoassays (ELISA) are difficult to directly reflect the enzymatic activity. Research indicates that future detection technologies will evolve towards a multi-methods integration approach (e.g., LC-MS combined with sensor arrays) and intelligent solutions (e.g., machine learning-assisted decision-making), while also addressing industry pain points such as blind spots in raw material screening, lag in online processing monitoring, and the absence of standards for end products. This paper provided a theoretical foundation for the construction of an efficient and precise thermoduric proteinase detection system, and has significant reference value for the establishing relevant industry standards and the optimization of quality control.

, authors=Shujie HU, authorsList=Shujie HU, authorCompany=null, correspAuthors=null, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1297134055829558131, articleId=1297134055384961905, tenantId=1146029695717560320, journalId=1296801528146919473, language=CN, title=乳制品中耐热蛋白酶的生物工程检测方法研究进展, columnId=1297134032135938673, journalTitle=中国乳业, columnName=质量安全, runingTitle=null, highlight=null, articleAbstract=

耐热蛋白酶是影响乳品品质稳定性的关键风险因子，其耐热特性导致常规杀菌工艺难以实现彻底灭活，进而引发产品在货架期内的蛋白质水解与感官品质劣变。本文系统分析了耐热蛋白酶的微生物来源（如假单胞菌、芽孢杆菌）及其热稳定机制（疏水相互作用、金属离子依赖性），并重点探讨了检测技术的创新进展与行业应用中所面临的瓶颈。现有检测方法中，比色法成本低但灵敏度不足，荧光检测技术（如FRET）虽灵敏度高却受限于底物通用性，而分子生物学（PCR）与免疫识别（ELISA）技术则难以直接反映酶活性。研究表明，未来检测技术将向多手段融合（如LC-MS联用传感器阵列）与智能化（如机器学习辅助决策）方向发展，同时需解决原料筛查盲区、加工在线监测滞后及终端产品标准缺失等产业痛点。本文为构建高效、精准的耐热蛋白酶检测体系提供了理论依据，对相关行业标准制定与质量控制优化有重要参考价值。

, authors=胡舒婕, authorsList=胡舒婕, authorCompany=null, correspAuthors=null, authorNote=, correspAuthorsNote=null, copyrightStatement=发表在《中国乳业》上的论文，视为将本论文的数字化复制权、发行权、汇编权及信息网络传播权许可给《中国乳业》使用。本刊已许可中国学术期刊（光盘版）电子杂志社、北京万方数据股份有限公司等多家单位在其相关数据库产品中以数字化方式复制、汇编、发行、信息网络传播本刊全文。作者向本刊提交文章发表的行为即视为同意我刊授权使用该文著作权。, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=IK2DMPhHQQ/OHzQDKj2IkQ==, magXml=dC0gamGdJ86jW733Ulzaiw==, pdfUrl=null, pdf=AoBfDMStHTD+i6yoOHE8MA==, pdfFileSize=807457, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=MfVT40xMk3wG0fsFHqhOxw==, mapNumber=null, fund=null)}, authors=[Author(id=1297134056173491064, tenantId=1146029695717560320, journalId=1296801528146919473, articleId=1297134055384961905, orderNo=0, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1297134056253182842, tenantId=1146029695717560320, journalId=1296801528146919473, articleId=1297134055384961905, authorId=1297134056173491064, language=EN, stringName=Shujie HU, firstName=Shujie, middleName=null, lastName=HU, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=null, address=Jiaozuo College of Industry And Trade, Jiaozuo Henan 454550, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1297134056324486011, tenantId=1146029695717560320, journalId=1296801528146919473, articleId=1297134055384961905, authorId=1297134056173491064, language=CN, stringName=胡舒婕, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=null, address=焦作工贸职业学院，河南 焦作 454550, bio={"content":"

胡舒婕（1996-），女，河南沁阳人，硕士，助教，研究方向为生物工程。

"}, bioImg=null, bioContent=

胡舒婕（1996-），女，河南沁阳人，硕士，助教，研究方向为生物工程。

, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1297134056077022068, tenantId=1146029695717560320, journalId=1296801528146919473, articleId=1297134055384961905, xref=null, ext=[AuthorCompanyExt(id=1297134056085410677, tenantId=1146029695717560320, journalId=1296801528146919473, articleId=1297134055384961905, companyId=1297134056077022068, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=Jiaozuo College of Industry And Trade, Jiaozuo Henan 454550), AuthorCompanyExt(id=1297134056093799286, tenantId=1146029695717560320, journalId=1296801528146919473, articleId=1297134055384961905, companyId=1297134056077022068, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=焦作工贸职业学院，河南 焦作 454550)])])], keywords=[Keyword(id=1297134056416760700, tenantId=1146029695717560320, journalId=1296801528146919473, articleId=1297134055384961905, language=EN, orderNo=1, keyword=dairy product), Keyword(id=1297134056475480957, tenantId=1146029695717560320, journalId=1296801528146919473, articleId=1297134055384961905, language=EN, orderNo=2, keyword=thermoduric proteinase), Keyword(id=1297134056534201214, tenantId=1146029695717560320, journalId=1296801528146919473, articleId=1297134055384961905, language=EN, orderNo=3, keyword=detection method), Keyword(id=1297134056613892991, tenantId=1146029695717560320, journalId=1296801528146919473, articleId=1297134055384961905, language=EN, orderNo=4, keyword=research progress), Keyword(id=1297134056689390464, tenantId=1146029695717560320, journalId=1296801528146919473, articleId=1297134055384961905, language=CN, orderNo=1, keyword=乳品), Keyword(id=1297134056760693633, tenantId=1146029695717560320, journalId=1296801528146919473, articleId=1297134055384961905, language=CN, orderNo=2, keyword=耐热蛋白酶), Keyword(id=1297134058379694978, tenantId=1146029695717560320, journalId=1296801528146919473, articleId=1297134055384961905, language=CN, orderNo=3, keyword=检测方法), Keyword(id=1297134058492941187, tenantId=1146029695717560320, journalId=1296801528146919473, articleId=1297134055384961905, language=CN, orderNo=4, keyword=研究进展)], refs=null, funds=null, companyList=[AuthorCompany(id=1297134056077022068, tenantId=1146029695717560320, journalId=1296801528146919473, articleId=1297134055384961905, xref=null, ext=[AuthorCompanyExt(id=1297134056085410677, tenantId=1146029695717560320, journalId=1296801528146919473, articleId=1297134055384961905, companyId=1297134056077022068, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=Jiaozuo College of Industry And Trade, Jiaozuo Henan 454550), AuthorCompanyExt(id=1297134056093799286, tenantId=1146029695717560320, journalId=1296801528146919473, articleId=1297134055384961905, companyId=1297134056077022068, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=焦作工贸职业学院，河南 焦作 454550)])], figs=null, attaches=null, journal=Journal(id=1296801172792901679, delFlag=0, nameCn=中国乳业, nameEn=China Dairy, nameHistory1=null, nameHistory2=null, issn=1671-4393, eissn=null, cn=11-4768/S, coden=null, periodic=0, language=CN, oaType=null, ccby=null, superviseOffice=null, ownerOffice=null, pubOffice=null, editorOffice=null, officeType=null, aims=null, clcCode=null, officeProv=null, officeCity=null, officeAddr=null, officeZip=null, officeEmail=null, officePhone=null, editDirector=null, officeDirector=null, officeDirectorPhone=null, officeStaffNum=null, officeEmpNum=null, coverPicUrl=fy0U6rTUhrcBdKszdYVe3w==, journalPrice=null, startedYear=null, abbrevIsoEn=China Dairy, journalRemark=null, publicationField=null, createdTime=1787111092997, updatedTime=1788949480572, createdBy=18614031015, updatedBy=13041195026, firstLetterCn=Z, firstLetterEn=Z, subjectCode=Agriculture, subjectName=null, subjectCodeEn=Agriculture, subjectNameEn=null, picCn=fy0U6rTUhrcBdKszdYVe3w==, picEn=1lm7RNSd456gWkkoaDGdEQ==, jcr=null, cjcr=null, exts=[JournalExt(id=1304511929255481461, language=CN, name=中国乳业, nameHistory1=null, nameHistory2=null, managedBy=, sponsoredBy=, publishedBy=, editorOffice=, officeProv=null, officeCity=null, officeAddr=, officeZip=, editDirector=, officeDirector=null, officePhone=null, coverPicUrl=null, journalRemark=, submitArticleUrl=null, websiteUrl=, createdTime=1788949480594, updatedTime=1788949480594, createdBy=13041195026, updatedBy=13041195026, submissionGuidelinesUrl=, submissionAuthorUrl=https://zgryauthor.cast.org.cn/webm, submissionEditorUrl=https://zgryeditor.cast.org.cn/webm, submissionReviewUrl=https://zgryauthor.cast.org.cn/webm, submissionCeEditorUrl=, submissionAeEditorUrl=, option={"copyright":""}), JournalExt(id=1304511929301618806, language=EN, name=China Dairy, nameHistory1=null, nameHistory2=null, managedBy=, sponsoredBy=, publishedBy=, editorOffice=, officeProv=null, officeCity=null, officeAddr=, officeZip=, editDirector=, officeDirector=null, officePhone=null, coverPicUrl=null, journalRemark=, submitArticleUrl=null, websiteUrl=, createdTime=1788949480605, updatedTime=1788949480605, createdBy=13041195026, updatedBy=13041195026, submissionGuidelinesUrl=, submissionAuthorUrl=https://zgryauthor.cast.org.cn/webm, submissionEditorUrl=https://zgryeditor.cast.org.cn/webm, submissionReviewUrl=https://zgryauthor.cast.org.cn/webm, submissionCeEditorUrl=, submissionAeEditorUrl=, option={"copyright":""})], databaseList=null, tenantJournalId=1296801528146919473, websiteList=[Website(id=1296802542923572121, webName=null, webTitle=null, webDomain=null, webCopyrigh=null, webIpcNo=null, seoTitle=null, seoKeywords=null, seoDescription=null, tenantJournalId=null, journalId=1296801528146919473, journalNameCn=null, journalNameEn=null, grayFlag=null, tenantId=1146029695717560320, platformId=null, journalGroupId=null, journalGroupNameCn=null, journalGroupNameEn=null, type=1, domain=https://castjournals.cast.org.cn/joweb/zgry/CN, language=CN, createTime=1787111419659, createBy=18614031015, updateTime=1787111471367, updateBy=18614031015, name=中国乳业-中文, tplId=1146099689490845704, title=中国乳业, delFlag=0, indexPage=/home, props=[WebsiteProps(id=1296802881777198042, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1296802542923572121, code=articleTextType, value=kx, createTime=1787111500448, updateTime=1787111500448, creator=18614031015, updator=18614031015), WebsiteProps(id=1296802881747837911, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1296802542923572121, code=banner, value=null, createTime=1787111500441, updateTime=1787111500441, creator=18614031015, updator=18614031015), WebsiteProps(id=1296802881827529693, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1296802542923572121, code=grayFlag, value=0, createTime=1787111500460, updateTime=1787111500460, creator=18614031015, updator=18614031015), WebsiteProps(id=1296802881735254998, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1296802542923572121, code=logo, value=https://castjournals.cast.org.cn/joweb/zgry/CN/file/pic?fileId=qVbzVKBQlSo4CZ2uDrqTZw==, createTime=1787111500438, updateTime=1787111500438, creator=18614031015, updator=18614031015), WebsiteProps(id=1296802881844306911, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1296802542923572121, code=minRunFlag, value=0, createTime=1787111500464, updateTime=1787111500464, creator=18614031015, updator=18614031015), WebsiteProps(id=1296802881768809433, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1296802542923572121, code=picServerUrl, value=https://castjournals.cast.org.cn/joweb/zgry/CN/file/pic, createTime=1787111500446, updateTime=1787111500446, creator=18614031015, updator=18614031015), WebsiteProps(id=1296802881835918302, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1296802542923572121, code=silenceFlag, value=0, createTime=1787111500462, updateTime=1787111500462, creator=18614031015, updator=18614031015), WebsiteProps(id=1296802881760420824, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1296802542923572121, code=staticResourcePath, value=https://castjournals.cast.org.cn/joweb/cast_kjdb_cn_619/, createTime=1787111500444, updateTime=1787111500444, creator=18614031015, updator=18614031015), WebsiteProps(id=1296802881810752475, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1296802542923572121, code=themeColor, value=null, createTime=1787111500456, updateTime=1787111500456, creator=18614031015, updator=18614031015), WebsiteProps(id=1296802881819141084, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1296802542923572121, code=themeStyle, value=null, createTime=1787111500458, updateTime=1787111500458, creator=18614031015, updator=18614031015)]), Website(id=1296802543032624028, webName=null, webTitle=null, webDomain=null, webCopyrigh=null, webIpcNo=null, seoTitle=null, seoKeywords=null, seoDescription=null, tenantJournalId=null, journalId=1296801528146919473, journalNameCn=null, journalNameEn=null, grayFlag=null, tenantId=1146029695717560320, platformId=null, journalGroupId=null, journalGroupNameCn=null, journalGroupNameEn=null, type=1, domain=https://castjournals.cast.org.cn/joweb/zgry/EN, language=EN, createTime=1787111419685, createBy=18614031015, updateTime=1787111462939, updateBy=18614031015, name=中国乳业-英文, tplId=1146101810881728533, title=China Dairy, delFlag=0, indexPage=/home, props=[WebsiteProps(id=1296802850798068681, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1296802543032624028, code=articleTextType, value=kx, createTime=1787111493062, updateTime=1787111493062, creator=18614031015, updator=18614031015), WebsiteProps(id=1296802850768708550, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1296802543032624028, code=banner, value=null, createTime=1787111493055, updateTime=1787111493055, creator=18614031015, updator=18614031015), WebsiteProps(id=1296802850819040204, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1296802543032624028, code=grayFlag, value=0, createTime=1787111493067, updateTime=1787111493067, creator=18614031015, updator=18614031015), WebsiteProps(id=1296802850760319941, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1296802543032624028, code=logo, value=https://castjournals.cast.org.cn/joweb/zgry/EN/file/pic?fileId=qVbzVKBQlSo4CZ2uDrqTZw==, createTime=1787111493053, updateTime=1787111493053, creator=18614031015, updator=18614031015), WebsiteProps(id=1296802850831623118, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1296802543032624028, code=minRunFlag, value=0, createTime=1787111493070, updateTime=1787111493070, creator=18614031015, updator=18614031015), WebsiteProps(id=1296802850789680072, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1296802543032624028, code=picServerUrl, value=https://castjournals.cast.org.cn/joweb/zgry/EN/file/pic, createTime=1787111493060, updateTime=1787111493060, creator=18614031015, updator=18614031015), WebsiteProps(id=1296802850823234509, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1296802543032624028, code=silenceFlag, value=0, createTime=1787111493068, updateTime=1787111493068, creator=18614031015, updator=18614031015), WebsiteProps(id=1296802850781291463, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1296802543032624028, code=staticResourcePath, value=https://castjournals.cast.org.cn/joweb/cast_kjdb_en_623/, createTime=1787111493058, updateTime=1787111493058, creator=18614031015, updator=18614031015), WebsiteProps(id=1296802850802262986, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1296802543032624028, code=themeColor, value=null, createTime=1787111493063, updateTime=1787111493063, creator=18614031015, updator=18614031015), WebsiteProps(id=1296802850810651595, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1296802543032624028, code=themeStyle, value=null, createTime=1787111493065, updateTime=1787111493065, creator=18614031015, updator=18614031015)])], journalTitle=中国乳业, weixinUrl=null, journalUrl=http://zhgry.aiijournal.com/, iacademicId=null, status=1, seqNo=null, journalTitleEn=China Dairy, journalPhotoCn=fy0U6rTUhrcBdKszdYVe3w==, journalPhotoEn=1lm7RNSd456gWkkoaDGdEQ==, journalFirstLetter=Z, journalRecommend=null, journalNew=null, journalCollection=null, jcrJf=null, cjcrJf=null, jcrJfStr=null, cjcrJfStr=null, submissionFirstDecision=null, sciSubjectClassification=null, casSubjectClassification=null, citeScore=null, totalCitationFrequency=null, icpCode=null, psCode=null, advertisingLicenseCode=null, copyrightInformation=null, country=null, option=, provinceCode=null, provinceName=null, collectFlag=false, interPubPlatform=, interPubPlatformUrl=null), detailUrlCn=https://castjournals.cast.org.cn/joweb/zgry/CN/10.12377/1671-4393.26.03.11, detailUrlEn=https://castjournals.cast.org.cn/joweb/zgry/EN/10.12377/1671-4393.26.03.11, pdfUrlCn=https://castjournals.cast.org.cn/joweb/zgry/CN/PDF/10.12377/1671-4393.26.03.11, pdfUrlEn=https://castjournals.cast.org.cn/joweb/zgry/EN/PDF/10.12377/1671-4393.26.03.11, aliStartDate=0, aliEndDate=0, collectionFlag=false, citedCount=null, citedUrl=null, previewStatus=0, delFlag=0, hasFullText=0, orderTime=1774368000000, fullTextJson=null, articleText=null, reference=null)