Article(id=1314923033891008993, tenantId=1146029695717560320, journalId=1234093305789726721, issueId=1314922986466009203, articleNumber=null, orderNo=null, doi=null, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1753372800000, receivedDateStr=2025-07-25, revisedDate=null, revisedDateStr=null, acceptedDate=null, acceptedDateStr=null, onlineDate=1791431681367, onlineDateStr=2026-10-08, pubDate=1773936000000, pubDateStr=2026-03-20, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1791431681367, onlineIssueDateStr=2026-10-08, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1791431681367, creator=13701087609, updateTime=1791431681367, updator=13701087609, issue=Issue{id=1314922986466009203, tenantId=1146029695717560320, journalId=1234093305789726721, year='2026', volume='46', issue='3', pageStart='1185', pageEnd='1776', issueExtLink='null', onlineDate='null', pubDate='1773936000000', pubDateStr='2026-03-20', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=1, specialIssue=null, createTime=1791431670061, creator='13701087609', updateTime=1791431955369, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1314924183251935526, tenantId=1146029695717560320, journalId=1234093305789726721, issueId=1314922986466009203, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1314924183251935527, tenantId=1146029695717560320, journalId=1234093305789726721, issueId=1314922986466009203, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=1331, endPage=1343, ext={EN=ArticleExt(id=1314923034230747618, articleId=1314923033891008993, tenantId=1146029695717560320, journalId=1234093305789726721, language=EN, title=Enhanced phosphorus removal in aerobic granular sludge system seeded with dewatered sludge pre-granules: Performance and mechanism, columnId=1234106386360103680, journalTitle=China Environmental Science, columnName=Water Pollution Control, runingTitle=null, highlight=null, articleAbstract=

In this study, dewatered sludge pre-granules were used as seed sludge to cultivate aerobic granular sludge (AGS) with high phosphorus removal capabilities over a 120-day operation. Inoculation with pre-granules allowed the system to bypass the initial granulation phase, establishing stable biological phosphorus removal by day 42. During the stable period, the experimental group achieved an average total phosphorus removal efficiency of (94.4±3.7)%, outperforming the control group (85.9±5.1)%. The pre-granulated AGS exhibited a particle size of 1100~1300μm, a sludge volume index of 50~80mL/g, and average removal efficiencies for chemical oxygen demand and total nitrogen of (94.5±2.9)% and (79.3±6.4)%, respectively. Scanning electron microscopy with energy-dispersive X-ray spectroscopy (SEM-EDS) revealed a notably higher iron content in the pre-granulated AGS (3.87% vs. 0.24% in the control), with iron uniformly distributed inside the granules. High-throughput sequencing further indicated enrichment of extracellular polymeric substance (EPS) secretion microorganisms, such as Candidatus_Competibacter (10.9% vs. 5.0% in the control). The results demonstrated that, unlike the primarily biological phosphorus removal mode in the control, the pre-granulated AGS developed a synergistic chemical-biological removal mechanism. Initially, EPS complexed with residual ferric iron from the dewatered sludge pre-granules, enhancing chemical phosphorus adsorption and providing a structural skeleton that improved granule stability. Subsequently, this stable structure facilitated microbial retention, yielding a higher mixed liquor volatile suspended solids concentration (4.3g/L vs.3.1g/L). Although the relative abundance of polyphosphate-accumulating organisms (PAOs) was similar between groups (0.48% vs. 0.47%), the absolute abundance of PAOs was elevated in the pre-granulated system. Moreover, the stable granular structure likely supported higher metabolic activity PAOs, as reflected by an increase in the relative abundance of key functional genes, such as polyphosphate kinase (ppk1, 0.42‰ vs. 0.34‰).

, authors=Hao-sheng YANG1, Hui-ping LI1, Xing-bo WANG1, Guang CHEN2, Li-hua WANG2, Yan LV2, Wei-hai PANG1, *, Li XIE1, Dian-hai YANG1, authorsList=Hao-sheng YANG, Hui-ping LI, Xing-bo WANG, Guang CHEN, Li-hua WANG, Yan LV, Wei-hai PANG, Li XIE, Dian-hai YANG, authorCompany=null, correspAuthors=Wei-hai PANG, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1314923034474017253, articleId=1314923033891008993, tenantId=1146029695717560320, journalId=1234093305789726721, language=CN, title=基于脱水污泥预造粒的好氧颗粒污泥系统除磷效能提升与机制, columnId=1234106386565624579, journalTitle=中国环境科学, columnName=水污染与控制, runingTitle=null, highlight=null, articleAbstract=

采用脱水污泥预造粒作为种子污泥,成功培养出高效除磷的好氧颗粒污泥(AGS),并持续运行了120d.结果表明,接种预造粒能够跳过初始成粒步骤,并在第42d实现生物除磷功能构建,稳定期平均总磷(TP)去除率达到(94.4±3.7)%,优于对照组的(85.9±5.1)%.同时,稳定期的预造粒AGS粒径达到1100~1300μm,污泥体积指数(SVI30)为50~80mL/g,化学需氧量(COD)和总氮(TN)的平均去除率分别达到(94.5±2.9)%和(79.3±6.4)%.扫描电镜结合能谱分析(SEM-EDS)表明,预造粒AGS富含铁元素(3.87% vs. 0.24%),且均匀分布在颗粒内部.进一步结合高通量测序,发现预造粒AGS中富集了Candidatus_Competibacter(10.9% vs. 5.0%)等具有胞外聚合物(EPS)分泌潜能的微生物.机制分析揭示,区别于对照组典型的生物驱动模式,预造粒AGS构建了化学-生物协同的高效除磷模式.运行初期,EPS与脱水污泥预造粒残留Fe3+络合,强化化学吸附除磷,并作为结构骨架增强颗粒稳定性.运行后期,该结构有助于微生物保留(混合液挥发性悬浮固体浓度MLVSS:4.3g/L vs.3.1g/L),使系统中聚磷菌(PAOs)在相对丰度与对照组相近(0.48% vs. 0.47%)的情况下,具有更高的生物量;同时稳定的结构亦可能有助于PAOs保持代谢活性,表现为多聚磷酸盐激酶(ppk1,0.42‰ vs. 0.34‰)等功能基因更高的相对丰度.

, authors=杨浩生1, 李惠平1, 王星博1, 陈广2, 王丽花2, 吕燕2, 庞维海1, *, 谢丽1, 杨殿海1, authorsList=杨浩生, 李惠平, 王星博, 陈广, 王丽花, 吕燕, 庞维海, 谢丽, 杨殿海, authorCompany=null, correspAuthors=庞维海, authorNote=

杨浩生(2001-),男,河南南阳人,同济大学硕士研究生,主要从事污水生物处理研究.发表论文1篇..

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杨浩生(2001-),男,河南南阳人,同济大学硕士研究生,主要从事污水生物处理研究.发表论文1篇..

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杨浩生(2001-),男,河南南阳人,同济大学硕士研究生,主要从事污水生物处理研究.发表论文1篇..

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基于脱水污泥预造粒的好氧颗粒污泥系统除磷效能提升与机制
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杨浩生 1 , 李惠平 1 , 王星博 1 , 陈广 2 , 王丽花 2 , 吕燕 2 , 庞维海 1, * , 谢丽 1 , 杨殿海 1
中国环境科学 | 水污染与控制 2026,46(3): 1331-1343
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中国环境科学 |水污染与控制 2026 , 46 (3) : 1331 -1343
基于脱水污泥预造粒的好氧颗粒污泥系统除磷效能提升与机制
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杨浩生(2001-),男,河南南阳人,同济大学硕士研究生,主要从事污水生物处理研究.发表论文1篇..

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杨浩生(2001-),男,河南南阳人,同济大学硕士研究生,主要从事污水生物处理研究.发表论文1篇..

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杨浩生1 , 李惠平1, 王星博1, 陈广2, 王丽花2, 吕燕2, 庞维海1, * , 谢丽1, 杨殿海1
作者信息
  • 1.同济大学环境科学与工程学院,长江水环境教育部重点实验室,上海 200092
  • 2.上海城投污水处理有限公司,上海 201203
通讯作者:
* 责任作者,讲师,
作者简介:

杨浩生(2001-),男,河南南阳人,同济大学硕士研究生,主要从事污水生物处理研究.发表论文1篇..

Enhanced phosphorus removal in aerobic granular sludge system seeded with dewatered sludge pre-granules: Performance and mechanism
Hao-sheng YANG1 , Hui-ping LI1, Xing-bo WANG1, Guang CHEN2, Li-hua WANG2, Yan LV2, Wei-hai PANG1, * , Li XIE1, Dian-hai YANG1
Affiliations
  • 1.Key Laboratory of Yangtze River Water Environment, Ministry of Education, School of Environmental Science and Engineering, Tongji University, Shanghai 200092, China
  • 2.Shanghai Chengtou Wastewater Treatment Co., Ltd., Shanghai 201203, China
出版时间: 2026-03-20
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采用脱水污泥预造粒作为种子污泥,成功培养出高效除磷的好氧颗粒污泥(AGS),并持续运行了120d.结果表明,接种预造粒能够跳过初始成粒步骤,并在第42d实现生物除磷功能构建,稳定期平均总磷(TP)去除率达到(94.4±3.7)%,优于对照组的(85.9±5.1)%.同时,稳定期的预造粒AGS粒径达到1100~1300μm,污泥体积指数(SVI30)为50~80mL/g,化学需氧量(COD)和总氮(TN)的平均去除率分别达到(94.5±2.9)%和(79.3±6.4)%.扫描电镜结合能谱分析(SEM-EDS)表明,预造粒AGS富含铁元素(3.87% vs. 0.24%),且均匀分布在颗粒内部.进一步结合高通量测序,发现预造粒AGS中富集了Candidatus_Competibacter(10.9% vs. 5.0%)等具有胞外聚合物(EPS)分泌潜能的微生物.机制分析揭示,区别于对照组典型的生物驱动模式,预造粒AGS构建了化学-生物协同的高效除磷模式.运行初期,EPS与脱水污泥预造粒残留Fe3+络合,强化化学吸附除磷,并作为结构骨架增强颗粒稳定性.运行后期,该结构有助于微生物保留(混合液挥发性悬浮固体浓度MLVSS:4.3g/L vs.3.1g/L),使系统中聚磷菌(PAOs)在相对丰度与对照组相近(0.48% vs. 0.47%)的情况下,具有更高的生物量;同时稳定的结构亦可能有助于PAOs保持代谢活性,表现为多聚磷酸盐激酶(ppk1,0.42‰ vs. 0.34‰)等功能基因更高的相对丰度.

好氧颗粒污泥  /  脱水污泥  /  预造粒  /  聚磷菌  /  生物除磷

In this study, dewatered sludge pre-granules were used as seed sludge to cultivate aerobic granular sludge (AGS) with high phosphorus removal capabilities over a 120-day operation. Inoculation with pre-granules allowed the system to bypass the initial granulation phase, establishing stable biological phosphorus removal by day 42. During the stable period, the experimental group achieved an average total phosphorus removal efficiency of (94.4±3.7)%, outperforming the control group (85.9±5.1)%. The pre-granulated AGS exhibited a particle size of 1100~1300μm, a sludge volume index of 50~80mL/g, and average removal efficiencies for chemical oxygen demand and total nitrogen of (94.5±2.9)% and (79.3±6.4)%, respectively. Scanning electron microscopy with energy-dispersive X-ray spectroscopy (SEM-EDS) revealed a notably higher iron content in the pre-granulated AGS (3.87% vs. 0.24% in the control), with iron uniformly distributed inside the granules. High-throughput sequencing further indicated enrichment of extracellular polymeric substance (EPS) secretion microorganisms, such as Candidatus_Competibacter (10.9% vs. 5.0% in the control). The results demonstrated that, unlike the primarily biological phosphorus removal mode in the control, the pre-granulated AGS developed a synergistic chemical-biological removal mechanism. Initially, EPS complexed with residual ferric iron from the dewatered sludge pre-granules, enhancing chemical phosphorus adsorption and providing a structural skeleton that improved granule stability. Subsequently, this stable structure facilitated microbial retention, yielding a higher mixed liquor volatile suspended solids concentration (4.3g/L vs.3.1g/L). Although the relative abundance of polyphosphate-accumulating organisms (PAOs) was similar between groups (0.48% vs. 0.47%), the absolute abundance of PAOs was elevated in the pre-granulated system. Moreover, the stable granular structure likely supported higher metabolic activity PAOs, as reflected by an increase in the relative abundance of key functional genes, such as polyphosphate kinase (ppk1, 0.42‰ vs. 0.34‰).

aerobic granular sludge  /  dewatered sludge  /  pre-granulation  /  polyphosphate-accumulating organism  /  biological phosphorus removal
杨浩生, 李惠平, 王星博, 陈广, 王丽花, 吕燕, 庞维海, 谢丽, 杨殿海. 基于脱水污泥预造粒的好氧颗粒污泥系统除磷效能提升与机制. 中国环境科学, 2026 , 46 (3) : 1331 -1343 .
Hao-sheng YANG, Hui-ping LI, Xing-bo WANG, Guang CHEN, Li-hua WANG, Yan LV, Wei-hai PANG, Li XIE, Dian-hai YANG. Enhanced phosphorus removal in aerobic granular sludge system seeded with dewatered sludge pre-granules: Performance and mechanism[J]. China Environmental Science, 2026 , 46 (3) : 1331 -1343 .
  • 上海市科学技术委员会基金项目(24230712500; 22230710500)
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  • 接收时间:2025-07-25
  • 首发时间:2026-10-08
  • 出版时间:2026-03-20
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  • 收稿日期:2025-07-25
基金
上海市科学技术委员会基金项目(24230712500; 22230710500)
作者信息
    1.同济大学环境科学与工程学院,长江水环境教育部重点实验室,上海 200092
    2.上海城投污水处理有限公司,上海 201203

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2种不同金属材料的力学参数
科
Family
属数
Number of
genus
种数
Number of
species
占总种数比例
Percentage of
total species (%)
属
Genus
种数
Number of
species
占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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