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This study employed Pseudomonas aeruginosa wild-type (WT), an exopolysaccharide (EPS)-overproducing strain (ΔmucA), and a flagellum-deficient mutant (ΔfliC) as model organisms. Combining the XDLVO theory, we systematically investigated the effects of bacterial surface structures, ionic strength, and chlorination on bacterial adhesion and chlorine resistance to polyethylene (PE) and polypropylene (PP) MPs. Key findings revealed that EPS secretion enhanced cell hydrophobicity (increasedγLW and decreased γAB in ΔmucA), significantly reducing the repulsive energy barrier (ΦT) and thereby promoting bacterial adhesion and survival on MPs. Flagella facilitated adhesion through electrostatic attraction and amplified the protective effect of MPs on bacteria. Ionic strength (NaCl) exhibited a dual effect: at ≤30mmol/L, double-layer compression reduced the repulsive barrier by 19%, markedly enhancing adhesion, whereas at 100mmol/L, Cl- adsorption induced charge reversal, inhibiting adhesion. Although chlorination (1.0mg/L) effectively inactivated free bacteria, it increased bacterial adhesion rates on MPs by 45%~75% through enhanced van der Waals attraction (), consequently elevating chlorine resistance(up to 2.48-fold).

, authors=Yu-xin HE, Dong-qin GONG, Xiao-le WEN, Guo-wei CHEN, Li LIU*, authorsList=Yu-xin HE, Dong-qin GONG, Xiao-le WEN, Guo-wei CHEN, Li LIU, authorCompany=null, correspAuthors=Li LIU, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1314922238860682122, articleId=1314922236511871876, tenantId=1146029695717560320, journalId=1234093305789726721, language=CN, title=细菌表面结构与离子强度对微塑料上病原菌附着及抗氯性的影响, columnId=1234106394006311784, journalTitle=中国环境科学, columnName=环境毒理与健康, runingTitle=null, highlight=null, articleAbstract=

本文以铜绿假单胞菌的野生型(WT)、胞外聚合物(EPS)分泌过量型(ΔmucA)和鞭毛缺失型(ΔfliC)为模型,结合XDLVO理论,系统研究了细菌表面结构、离子强度和氯消毒对聚乙烯(PE)和聚丙烯(PP)MPs表面细菌附着及抗氯性的影响.结果表明,EPS分泌增强了细胞疏水性(ΔmucA的γLW升高、γAB降低),显著降低排斥势垒φT,进而促进细菌在MPs上的附着与存活;鞭毛则通过静电吸引作用增强附着能力,并提升MPs对细菌的保护效应.离子强度(NaCl)对细菌附着呈双重效应:在NaCl浓度≤30mmol/L时,双电层压缩使排斥势垒降至19%,显著促进附着;而在100mmol/L时,Cl-吸附导致电荷反转,从而抑制附着.尽管氯消毒(1.0mg/L)可有效灭活游离菌体,但其通过增强范德华吸附力()显著提高了细菌在MPs表面的附着率(提升至45%~75%),从而增强其抗氯性(最高达2.48倍).

, authors=何豫馨, 龚东琴, 温晓乐, 陈国炜, 刘丽*, authorsList=何豫馨, 龚东琴, 温晓乐, 陈国炜, 刘丽, authorCompany=null, correspAuthors=刘丽, authorNote=

何豫馨(2002-),女,四川乐山人,合肥工业大学硕士研究生,主要研究为微塑料对细菌抗生素抗性传播的影响.发表论文1篇..

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* 责任作者,教授,
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何豫馨(2002-),女,四川乐山人,合肥工业大学硕士研究生,主要研究为微塑料对细菌抗生素抗性传播的影响.发表论文1篇..

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何豫馨(2002-),女,四川乐山人,合肥工业大学硕士研究生,主要研究为微塑料对细菌抗生素抗性传播的影响.发表论文1篇..

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细菌表面结构与离子强度对微塑料上病原菌附着及抗氯性的影响
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何豫馨 , 龚东琴 , 温晓乐 , 陈国炜 , 刘丽 *
中国环境科学 | 环境毒理与健康 2026,46(2): 1038-1046
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中国环境科学 |环境毒理与健康 2026 , 46 (2) : 1038 -1046
细菌表面结构与离子强度对微塑料上病原菌附着及抗氯性的影响
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何豫馨(2002-),女,四川乐山人,合肥工业大学硕士研究生,主要研究为微塑料对细菌抗生素抗性传播的影响.发表论文1篇..

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何豫馨(2002-),女,四川乐山人,合肥工业大学硕士研究生,主要研究为微塑料对细菌抗生素抗性传播的影响.发表论文1篇..

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何豫馨 , 龚东琴, 温晓乐, 陈国炜, 刘丽*
作者信息
  • 合肥工业大学土木与水利工程学院,安徽 合肥 230009
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* 责任作者,教授,
作者简介:

何豫馨(2002-),女,四川乐山人,合肥工业大学硕士研究生,主要研究为微塑料对细菌抗生素抗性传播的影响.发表论文1篇..

Effects of bacterial surface structures and ionic strength on pathogen adhesion to microplastics and their chlorine resistance
Yu-xin HE , Dong-qin GONG, Xiao-le WEN, Guo-wei CHEN, Li LIU*
Affiliations
  • Department of Civil Engineering, Hefei University of Technology, Hefei 230009, China
出版时间: 2026-02-20
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本文以铜绿假单胞菌的野生型(WT)、胞外聚合物(EPS)分泌过量型(ΔmucA)和鞭毛缺失型(ΔfliC)为模型,结合XDLVO理论,系统研究了细菌表面结构、离子强度和氯消毒对聚乙烯(PE)和聚丙烯(PP)MPs表面细菌附着及抗氯性的影响.结果表明,EPS分泌增强了细胞疏水性(ΔmucA的γLW升高、γAB降低),显著降低排斥势垒φT,进而促进细菌在MPs上的附着与存活;鞭毛则通过静电吸引作用增强附着能力,并提升MPs对细菌的保护效应.离子强度(NaCl)对细菌附着呈双重效应:在NaCl浓度≤30mmol/L时,双电层压缩使排斥势垒降至19%,显著促进附着;而在100mmol/L时,Cl-吸附导致电荷反转,从而抑制附着.尽管氯消毒(1.0mg/L)可有效灭活游离菌体,但其通过增强范德华吸附力()显著提高了细菌在MPs表面的附着率(提升至45%~75%),从而增强其抗氯性(最高达2.48倍).

微塑料  /  胞外聚合物  /  鞭毛  /  抗氯性  /  饮用水

This study employed Pseudomonas aeruginosa wild-type (WT), an exopolysaccharide (EPS)-overproducing strain (ΔmucA), and a flagellum-deficient mutant (ΔfliC) as model organisms. Combining the XDLVO theory, we systematically investigated the effects of bacterial surface structures, ionic strength, and chlorination on bacterial adhesion and chlorine resistance to polyethylene (PE) and polypropylene (PP) MPs. Key findings revealed that EPS secretion enhanced cell hydrophobicity (increasedγLW and decreased γAB in ΔmucA), significantly reducing the repulsive energy barrier (ΦT) and thereby promoting bacterial adhesion and survival on MPs. Flagella facilitated adhesion through electrostatic attraction and amplified the protective effect of MPs on bacteria. Ionic strength (NaCl) exhibited a dual effect: at ≤30mmol/L, double-layer compression reduced the repulsive barrier by 19%, markedly enhancing adhesion, whereas at 100mmol/L, Cl- adsorption induced charge reversal, inhibiting adhesion. Although chlorination (1.0mg/L) effectively inactivated free bacteria, it increased bacterial adhesion rates on MPs by 45%~75% through enhanced van der Waals attraction (), consequently elevating chlorine resistance(up to 2.48-fold).

microplastics  /  extracellular polymeric substances (EPS)  /  flagella  /  chlorine resistance  /  drinking water
何豫馨, 龚东琴, 温晓乐, 陈国炜, 刘丽. 细菌表面结构与离子强度对微塑料上病原菌附着及抗氯性的影响. 中国环境科学, 2026 , 46 (2) : 1038 -1046 .
Yu-xin HE, Dong-qin GONG, Xiao-le WEN, Guo-wei CHEN, Li LIU. Effects of bacterial surface structures and ionic strength on pathogen adhesion to microplastics and their chlorine resistance[J]. China Environmental Science, 2026 , 46 (2) : 1038 -1046 .
  • 安徽省自然科学基金资助项目(2508085ME116)
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  • 接收时间:2025-07-20
  • 首发时间:2026-10-08
  • 出版时间:2026-02-20
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  • 收稿日期:2025-07-20
基金
安徽省自然科学基金资助项目(2508085ME116)
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    合肥工业大学土木与水利工程学院,安徽 合肥 230009

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2种不同金属材料的力学参数
科
Family
属数
Number of
genus
种数
Number of
species
占总种数比例
Percentage of
total species (%)
属
Genus
种数
Number of
species
占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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