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The rapid startup and stable operation of the partial denitrification/anammox (PD/A) system remain challenges limiting their engineering application in wastewater nitrogen removal. This study investigates the startup process of a PD/A reactor, inoculated with an aerobic sludge biofilm, under conditions of low substrate concentrations (NH4+-N < 50mg/L, NO3--N < 30mg/L) and a C/N ratio of 2.86. After 100d of operation, the system successfully enriched both anaerobic ammonium-oxidizing bacteria (AnAOB) and partial denitrification bacteria (PDB), achieving stable operation. By the end of Phase Ⅳ, the total nitrogen removal rate (Re. TN) and nitrogen removal rate (NRR) stabilized at(78.42±3.78)% and (2554.58±132.78)mgTN/(m2·d), respectively. The activity of PDB remained consistently higher than that of AnAOB, reaching stabilization levels of (6495.85±627.61) and (3093.15±43.91) mgN/(m2·d), respectively. High-throughput sequencing revealed significant enrichment of PDB (Thauera, Denitratisoma) and AnAOB (Ca. Brocadia, Ca. Jettenia), with their relative abundances being 5.33%, 8.32%, 13.57%, and 0.29%, respectively. Fluorescence in situ hybridization (FISH) analysis indicated that AnAOB predominantly colonized the outer layer of the biofilm, suggesting a chemotactic migration behavior. Partial least squares path modeling (PLS-PM) analysis showed that operational conditions directly influenced microbial community succession, which, in turn, indirectly affected the nitrogen removal performance of the system. This study offers valuable theoretical insights and engineering references for the rapid startup and stable operation of PD/A systems.

, authors=Na WANG1, Pei-yun ZHANG2, Si-jia GUO1, Kai LV1, *, Dang-cong PENG3, authorsList=Na WANG, Pei-yun ZHANG, Si-jia GUO, Kai LV, Dang-cong PENG, authorCompany=null, correspAuthors=Kai LV, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1314918508702556796, articleId=1314918508236989050, tenantId=1146029695717560320, journalId=1234093305789726721, language=CN, title=低氨氮浓度下PD/A系统快速启动与脱氮性能驱动机制研究, columnId=1234106386565624579, journalTitle=中国环境科学, columnName=水污染与控制, runingTitle=null, highlight=null, articleAbstract=

部分反硝化厌氧氨氧化(PD/A)系统的快速启动和稳定运行是限制其在污水脱氮工程化应用的主要问题.本研究采用接种好氧池生物膜,并在低基质浓度(NH4+-N<50mg/L, NO3--N<30mg/L)及C/N(2.86)的条件下,同时富集厌氧氨氧化菌(AnAOB)和部分反硝化菌(PDB),经过100d运行成功启动PD/A反应器.系统稳定运行后(第Ⅳ阶段末期),总氮去除率(Re.TN)和氮去除速率(NRR)达(78.42±3.78)%和(2554.58±132.78)mgTN/(m2·d).PDB活性始终高于AnAOB 活性,并最终分别稳定在(6459.85 ± 627.61)和(3093.15 ± 43.91)mgN/(m2·d).高通量测序结果显示,PDB(Thauera、Denitratisoma)和AnAOB(Ca.Brocadia、Ca.Jettenia)均得到富集,其相对丰度分别为5.33%、8.32%和13.57%、0.29%.荧光原位杂交(FISH)分析显示,AnAOB主要分布于生物膜外侧,表现出“趋化性定殖”特征.偏最小二乘路径模型(PLS-PM)分析表明,操作条件可直接影响微生物群落的演替,并通过调控微生物组成间接影响系统的脱氮性能.本研究为PD/A系统的快速启动与稳定运行提供了理论支持和工程参考.

, authors=王娜1, 张佩云2, 郭思嘉1, 吕恺1, *, 彭党聪3, authorsList=王娜, 张佩云, 郭思嘉, 吕恺, 彭党聪, authorCompany=null, correspAuthors=吕恺, authorNote=

王娜(2001-),女,宁夏吴忠人,宁夏大学硕士研究生,主要从事污水生物处理研究.发表论文1篇..

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王娜(2001-),女,宁夏吴忠人,宁夏大学硕士研究生,主要从事污水生物处理研究.发表论文1篇..

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王娜(2001-),女,宁夏吴忠人,宁夏大学硕士研究生,主要从事污水生物处理研究.发表论文1篇..

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低氨氮浓度下PD/A系统快速启动与脱氮性能驱动机制研究
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王娜 1 , 张佩云 2 , 郭思嘉 1 , 吕恺 1, * , 彭党聪 3
中国环境科学 | 水污染与控制 2025,45(12): 6631-6640
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中国环境科学 |水污染与控制 2025 , 45 (12) : 6631 -6640
低氨氮浓度下PD/A系统快速启动与脱氮性能驱动机制研究
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王娜(2001-),女,宁夏吴忠人,宁夏大学硕士研究生,主要从事污水生物处理研究.发表论文1篇..

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王娜(2001-),女,宁夏吴忠人,宁夏大学硕士研究生,主要从事污水生物处理研究.发表论文1篇..

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王娜1 , 张佩云2, 郭思嘉1, 吕恺1, * , 彭党聪3
作者信息
  • 1.宁夏大学生态环境学院,宁夏 银川 750021
  • 2.宁夏环境科学研究院(有限责任公司),宁夏 银川 750011
  • 3.西安建筑科技大学环境与市政工程学院,陕西 西安 710055
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* 责任作者,讲师,
作者简介:

王娜(2001-),女,宁夏吴忠人,宁夏大学硕士研究生,主要从事污水生物处理研究.发表论文1篇..

The rapid startup and nitrogen removal performance driving mechanisms of the PD/A system
Na WANG1 , Pei-yun ZHANG2, Si-jia GUO1, Kai LV1, * , Dang-cong PENG3
Affiliations
  • 1.School of Ecological and Environmental Engineering, Ningxia University, Yinchuan 750021, China
  • 2.Ningxia Institute of Environmental Science (Limited Liability Company), Yinchuan 750011, China
  • 3.School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China
出版时间: 2025-12-20
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部分反硝化厌氧氨氧化(PD/A)系统的快速启动和稳定运行是限制其在污水脱氮工程化应用的主要问题.本研究采用接种好氧池生物膜,并在低基质浓度(NH4+-N<50mg/L, NO3--N<30mg/L)及C/N(2.86)的条件下,同时富集厌氧氨氧化菌(AnAOB)和部分反硝化菌(PDB),经过100d运行成功启动PD/A反应器.系统稳定运行后(第Ⅳ阶段末期),总氮去除率(Re.TN)和氮去除速率(NRR)达(78.42±3.78)%和(2554.58±132.78)mgTN/(m2·d).PDB活性始终高于AnAOB 活性,并最终分别稳定在(6459.85 ± 627.61)和(3093.15 ± 43.91)mgN/(m2·d).高通量测序结果显示,PDB(Thauera、Denitratisoma)和AnAOB(Ca.Brocadia、Ca.Jettenia)均得到富集,其相对丰度分别为5.33%、8.32%和13.57%、0.29%.荧光原位杂交(FISH)分析显示,AnAOB主要分布于生物膜外侧,表现出“趋化性定殖”特征.偏最小二乘路径模型(PLS-PM)分析表明,操作条件可直接影响微生物群落的演替,并通过调控微生物组成间接影响系统的脱氮性能.本研究为PD/A系统的快速启动与稳定运行提供了理论支持和工程参考.

低氨氮废水  /  厌氧氨氧化  /  部分反硝化厌氧氨氧化  /  荧光原位杂交  /  偏最小二乘路径模型  /  启动及运行特性

The rapid startup and stable operation of the partial denitrification/anammox (PD/A) system remain challenges limiting their engineering application in wastewater nitrogen removal. This study investigates the startup process of a PD/A reactor, inoculated with an aerobic sludge biofilm, under conditions of low substrate concentrations (NH4+-N < 50mg/L, NO3--N < 30mg/L) and a C/N ratio of 2.86. After 100d of operation, the system successfully enriched both anaerobic ammonium-oxidizing bacteria (AnAOB) and partial denitrification bacteria (PDB), achieving stable operation. By the end of Phase Ⅳ, the total nitrogen removal rate (Re. TN) and nitrogen removal rate (NRR) stabilized at(78.42±3.78)% and (2554.58±132.78)mgTN/(m2·d), respectively. The activity of PDB remained consistently higher than that of AnAOB, reaching stabilization levels of (6495.85±627.61) and (3093.15±43.91) mgN/(m2·d), respectively. High-throughput sequencing revealed significant enrichment of PDB (Thauera, Denitratisoma) and AnAOB (Ca. Brocadia, Ca. Jettenia), with their relative abundances being 5.33%, 8.32%, 13.57%, and 0.29%, respectively. Fluorescence in situ hybridization (FISH) analysis indicated that AnAOB predominantly colonized the outer layer of the biofilm, suggesting a chemotactic migration behavior. Partial least squares path modeling (PLS-PM) analysis showed that operational conditions directly influenced microbial community succession, which, in turn, indirectly affected the nitrogen removal performance of the system. This study offers valuable theoretical insights and engineering references for the rapid startup and stable operation of PD/A systems.

low ammonium nitrogen wastewater  /  anaerobic ammonium oxidation  /  partial denitrification/anammox  /  fluorescence in situ hybridization  /  partial least squares path modeling  /  startup and operational characteristics
王娜, 张佩云, 郭思嘉, 吕恺, 彭党聪. 低氨氮浓度下PD/A系统快速启动与脱氮性能驱动机制研究. 中国环境科学, 2025 , 45 (12) : 6631 -6640 .
Na WANG, Pei-yun ZHANG, Si-jia GUO, Kai LV, Dang-cong PENG. The rapid startup and nitrogen removal performance driving mechanisms of the PD/A system[J]. China Environmental Science, 2025 , 45 (12) : 6631 -6640 .
  • 宁夏自然科学基金资助项目(2024AAC05031)
  • 宁夏回族自治区河湖水环境水生态创新团队项目(2024CXTD016)
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  • 接收时间:2025-04-11
  • 首发时间:2026-10-08
  • 出版时间:2025-12-20
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  • 收稿日期:2025-04-11
基金
宁夏自然科学基金资助项目(2024AAC05031)
宁夏回族自治区河湖水环境水生态创新团队项目(2024CXTD016)
作者信息
    1.宁夏大学生态环境学院,宁夏 银川 750021
    2.宁夏环境科学研究院(有限责任公司),宁夏 银川 750011
    3.西安建筑科技大学环境与市政工程学院,陕西 西安 710055

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2种不同金属材料的力学参数
科
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属数
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genus
种数
Number of
species
占总种数比例
Percentage of
total species (%)
属
Genus
种数
Number of
species
占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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