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Eutrophication-induced algal blooms significantly disrupt the stability of aquatic ecosystems. Micro-nano bubbles (MNBs), which generate reactive oxygen species (ROS) upon self-collapse, exhibit strong oxidative capability and hold great promise for water pollution control. This study investigates the patterns and impact factors of ROS generation by MNBs under various environmental conditions, and further explores the role of ROS in the degradation of Chlorella. The results demonstrate that higher gas flow rates, longer aeration times, and greater water depths favor the generation of ROS, with hydroxyl radicals (·OH) identified as the primary active species. Degradation experiments reveal that, compared to conventional ozone bubbles (O3), O3-MNBs produce a higher yield of ROS, which further induce oxidative damage to the Chlorella cell membrane, leading to accelerated leakage of intracellular proteins and nucleic acids, and ultimately causing cell lysis. ROS inhibition studies confirm that ·OH is the key driver of cell membrane damage in Chlorella. This study provides insights into the generation mechanisms of ROS by MNBs, elucidates their roles in algal inactivation, and offers valuable theoretical support for the management of eutrophic water bodies.
, authors=Peng-yu ZHOU
1, 2, Liang HUANG
3, Xin-ze TAN
1, 2, Yao-hua LI
1, 2, Jun LUO
1, 2, Hong-feng CHEN
1, 2, *, authorsList=Peng-yu ZHOU, Liang HUANG, Xin-ze TAN, Yao-hua LI, Jun LUO, Hong-feng CHEN, authorCompany=null, correspAuthors=Hong-feng CHEN, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1314918503803609668, articleId=1314918503300293186, tenantId=1146029695717560320, journalId=1234093305789726721, language=CN, title=微纳米气泡产ROS规律及其对藻灭活机理分析, columnId=1234106386565624579, journalTitle=中国环境科学, columnName=水污染与控制, runingTitle=null, highlight=null, articleAbstract=
本研究分析了不同环境条件下微纳米气泡(MNBs)生成活性氧(ROS)的规律与影响因素,并进一步研究了ROS在小球藻失活中的作用机制.结果表明,较高气流量、长曝气时间和较大水深条件下更有利于ROS的产生,其中羟基自由基(·OH)是MNBs主要的活性物种.灭活实验表明,与常规臭氧气泡(O3)相比,O3-MNBs产生更多的ROS可进一步诱导小球藻细胞膜氧化损伤,加速胞内蛋白和核酸泄漏,最终导致藻体裂解.ROS抑制实验进一步证实·OH是小球藻细胞膜损伤的关键驱动力.本研究揭示了MNBs产ROS的规律及影响因素,并阐明了MNBs对藻的灭活效能及机理,为富营养化水体治理提供了理论支持.
, authors=周鹏宇
1, 2, 黄亮
3, 檀鑫泽
1, 2, 李耀华
1, 2, 罗俊
1, 2, 陈红凤
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周鹏宇(2001-),男,湖北武汉人,三峡大学水利与环境学院硕士研究生,研究方向为生态水利.857379428@qq.com.
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