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An airlift internal circulation reactor was used, and laboratory-cultivated full-nitrification granular sludge was inoculated into a continuous-flow reactor. During long-term operation, a suppression strategy based on a free nitrous acid (FNA) concentration of 150mg/L formed at pH 2.5 was applied to investigate the impact of high FNA concentrations under weak acid conditions on the nitritation of granular sludge. The results showed that this suppression strategy strongly inhibited both aerobic ammonia-oxidizing bacteria (AOB) and nitrite-oxidizing bacteria (NOB), but AOB exhibited a greater recovery capacity than NOB, enabling the successful initiation of nitritation in the granular sludge reactor within one day. During stable operation, the ammonium removal rate (ARR) reached 0.2~0.5kg/(m3·d), and the nitrite accumulation efficiency (NAE) reached 100%. However, the granular sludge nitritation process exhibited a cyclical deterioration pattern during long-term operation, necessitating periodic repetition of the suppression strategy. Under weak acid conditions, the secretion of extracellular polymeric substances from granular sludge gradually decreased, leading to the continuous deterioration of its structure. High-throughput sequencing results indicated that throughout the duration of the experiment, the Nitrosomonas genus consistently dominated the AOB community in the granular sludge, while the relative abundance of the NOB genus Candidatus_Nitrotoga decreased from 5.2% to approximately 0%.

, authors=Jing LONG1, Chao-wen XIE1, Hao-ting TANG1, Ming-jun LI1, Wen-ru LIU1, 2, *, authorsList=Jing LONG, Chao-wen XIE, Hao-ting TANG, Ming-jun LI, Wen-ru LIU, authorCompany=null, correspAuthors=Wen-ru LIU, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1314918378624606828, articleId=1314918378221953642, tenantId=1146029695717560320, journalId=1234093305789726721, language=CN, title=弱酸条件下基于FNA抑制的颗粒污泥亚硝化工艺效能研究, columnId=1234106386565624579, journalTitle=中国环境科学, columnName=水污染与控制, runingTitle=null, highlight=null, articleAbstract=

采用气提式内循环反应器,在连续流反应器内接种实验室培养的全硝化颗粒污泥,在长期运行中使用基于pH 2.5形成的FNA(游离亚硝酸)抑制浓度为150mg/L的抑制策略,探究弱酸条件下超高浓度FNA抑制对颗粒污泥亚硝化的影响.结果表明,该抑制策略对好氧氨氧化菌(AOB)和亚硝酸盐氧化菌(NOB)均抑制强烈,但AOB有着强于NOB的恢复能力,使得颗粒污泥反应器中亚硝化在1d内成功启动;稳定运行过程中氨氮去除负荷(ARR)达到0.2~0.5kg/(m3·d),亚硝酸盐积累率(NAE)达到100%.然而,颗粒污泥亚硝化工艺长期运行过程中呈现出周期性恶化规律,需要定期重复实施该抑制策略.弱酸条件下颗粒污泥胞外聚合物分泌逐渐减少,颗粒污泥结构不断恶化.高通量测序结果显示,整个实验过程中颗粒污泥内AOB始终以Nitrosomonas属为主导,NOB菌属Candidatus_Nitrotoga的相对丰度从5.2%降低至约0%.

, authors=隆静1, 解朝雯1, 汤皓婷1, 李明骏1, 刘文如1, 2, *, authorsList=隆静, 解朝雯, 汤皓婷, 李明骏, 刘文如, authorCompany=null, correspAuthors=刘文如, authorNote=

隆静(2000-),女,陕西宝鸡人,苏州科技大学硕士研究生,研究方向为废水处理及其资源化.发表论文4篇..

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隆静(2000-),女,陕西宝鸡人,苏州科技大学硕士研究生,研究方向为废水处理及其资源化.发表论文4篇..

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隆静(2000-),女,陕西宝鸡人,苏州科技大学硕士研究生,研究方向为废水处理及其资源化.发表论文4篇..

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弱酸条件下基于FNA抑制的颗粒污泥亚硝化工艺效能研究
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隆静 1 , 解朝雯 1 , 汤皓婷 1 , 李明骏 1 , 刘文如 1, 2, *
中国环境科学 | 水污染与控制 2025,45(11): 6128-6136
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中国环境科学 |水污染与控制 2025 , 45 (11) : 6128 -6136
弱酸条件下基于FNA抑制的颗粒污泥亚硝化工艺效能研究
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隆静(2000-),女,陕西宝鸡人,苏州科技大学硕士研究生,研究方向为废水处理及其资源化.发表论文4篇..

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隆静(2000-),女,陕西宝鸡人,苏州科技大学硕士研究生,研究方向为废水处理及其资源化.发表论文4篇..

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隆静1 , 解朝雯1, 汤皓婷1, 李明骏1, 刘文如1, 2, *
作者信息
  • 1.苏州科技大学环境科学与工程学院,江苏 苏州 215009
  • 2.城市生活污水资源化利用技术国家地方联合工程实验室,江苏省水处理技术与材料协同创新中心,江苏 苏州 215009
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* 责任作者, 副教授,
作者简介:

隆静(2000-),女,陕西宝鸡人,苏州科技大学硕士研究生,研究方向为废水处理及其资源化.发表论文4篇..

Performance of granular sludge-based nitritation with FNA inhibition under weakly acidic conditions
Jing LONG1 , Chao-wen XIE1, Hao-ting TANG1, Ming-jun LI1, Wen-ru LIU1, 2, *
Affiliations
  • 1.School of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou 215009, China
  • 2.Jiangsu Collaborative Innovation Center of Technology and Material of Water Treatment, National and Local Joint Engineering Laboratory of Municipal Sewage Resource Utilization Technology, Suzhou 215009, China
出版时间: 2025-11-20
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采用气提式内循环反应器,在连续流反应器内接种实验室培养的全硝化颗粒污泥,在长期运行中使用基于pH 2.5形成的FNA(游离亚硝酸)抑制浓度为150mg/L的抑制策略,探究弱酸条件下超高浓度FNA抑制对颗粒污泥亚硝化的影响.结果表明,该抑制策略对好氧氨氧化菌(AOB)和亚硝酸盐氧化菌(NOB)均抑制强烈,但AOB有着强于NOB的恢复能力,使得颗粒污泥反应器中亚硝化在1d内成功启动;稳定运行过程中氨氮去除负荷(ARR)达到0.2~0.5kg/(m3·d),亚硝酸盐积累率(NAE)达到100%.然而,颗粒污泥亚硝化工艺长期运行过程中呈现出周期性恶化规律,需要定期重复实施该抑制策略.弱酸条件下颗粒污泥胞外聚合物分泌逐渐减少,颗粒污泥结构不断恶化.高通量测序结果显示,整个实验过程中颗粒污泥内AOB始终以Nitrosomonas属为主导,NOB菌属Candidatus_Nitrotoga的相对丰度从5.2%降低至约0%.

好氧颗粒污泥  /  连续流反应器  /  亚硝化  /  FNA抑制  /  Nitrosomonas

An airlift internal circulation reactor was used, and laboratory-cultivated full-nitrification granular sludge was inoculated into a continuous-flow reactor. During long-term operation, a suppression strategy based on a free nitrous acid (FNA) concentration of 150mg/L formed at pH 2.5 was applied to investigate the impact of high FNA concentrations under weak acid conditions on the nitritation of granular sludge. The results showed that this suppression strategy strongly inhibited both aerobic ammonia-oxidizing bacteria (AOB) and nitrite-oxidizing bacteria (NOB), but AOB exhibited a greater recovery capacity than NOB, enabling the successful initiation of nitritation in the granular sludge reactor within one day. During stable operation, the ammonium removal rate (ARR) reached 0.2~0.5kg/(m3·d), and the nitrite accumulation efficiency (NAE) reached 100%. However, the granular sludge nitritation process exhibited a cyclical deterioration pattern during long-term operation, necessitating periodic repetition of the suppression strategy. Under weak acid conditions, the secretion of extracellular polymeric substances from granular sludge gradually decreased, leading to the continuous deterioration of its structure. High-throughput sequencing results indicated that throughout the duration of the experiment, the Nitrosomonas genus consistently dominated the AOB community in the granular sludge, while the relative abundance of the NOB genus Candidatus_Nitrotoga decreased from 5.2% to approximately 0%.

aerobic granular sludge  /  continuous flow reactor  /  nitritation  /  FNA inhibition  /  Nitrosomonas
隆静, 解朝雯, 汤皓婷, 李明骏, 刘文如. 弱酸条件下基于FNA抑制的颗粒污泥亚硝化工艺效能研究. 中国环境科学, 2025 , 45 (11) : 6128 -6136 .
Jing LONG, Chao-wen XIE, Hao-ting TANG, Ming-jun LI, Wen-ru LIU. Performance of granular sludge-based nitritation with FNA inhibition under weakly acidic conditions[J]. China Environmental Science, 2025 , 45 (11) : 6128 -6136 .
  • 国家自然科学基金资助项目(52470090)
  • 江苏省研究生实践创新计划项目(SJCX23_1706)
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  • 接收时间:2025-04-14
  • 首发时间:2026-10-08
  • 出版时间:2025-11-20
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  • 收稿日期:2025-04-14
基金
国家自然科学基金资助项目(52470090)
江苏省研究生实践创新计划项目(SJCX23_1706)
作者信息
    1.苏州科技大学环境科学与工程学院,江苏 苏州 215009
    2.城市生活污水资源化利用技术国家地方联合工程实验室,江苏省水处理技术与材料协同创新中心,江苏 苏州 215009

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* 责任作者, 副教授,
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2种不同金属材料的力学参数
科
Family
属数
Number of
genus
种数
Number of
species
占总种数比例
Percentage of
total species (%)
属
Genus
种数
Number of
species
占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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