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When typical triphenylmethane dyes (crystal violet) and phenazine dyes (saffron T) were used as the research objects, the biotoxicity of dye wastewater was investigated before and after treatment using the Fe⁰/H₂O₂ process through the analysis in growth indicators (growth rate and inhibition rate), photosynthetic indicators (chlorophyll a, chlorophyll b, and carotenoid content), protein content, polysaccharide content, and antioxidant enzyme activity systems (superoxide dismutase (SOD), catalase (CAT), and malondialdehyde (MDA)) of Chlorella vulgaris (C.vulgaris). The results showed that with the extension of reaction time, the growth rate of C.vulgaris gradually decreased and its inhibition rate increased. The growth of C.vulgaris cells was significantly inhibited in raw dye wastewater. Compared with raw dye wastewater, chlorophyll-A content of C.vulgaris was increased by 22.01% in the effluent wastewater after treatment, and the contents of chlorophyll-B and carotenoid were decreased by 12.97% and 16.02%, respectively. The contents of SOD and CAT were increased by 15.13% and 12.5%, respectively, while the content of MDA was decreased by 23.97%, respectively. The protein content of C.vulgaris in raw dye wastewater was low, while its content was high in the effluent wastewater after treatment. Compared with raw dye wastewater, the content of polysaccharide in the effluent wastewater exhibited a decreasing trend. According to the toxicity prediction of algae using EPI Suite software, the toxicity of the intermediate product of crystal violet and saffron T degradation to fish, water flea and green algae was significantly reduced. Therefore, the biotoxicity of dye wastewater could be effectively reduced in the Fe⁰/H₂O₂ system.

, authors=Yan-ping JIA^*, Yan-ju MA, Ru ZHENG, Jia-wei GUO, Lan-he ZHANG, authorsList=Yan-ping JIA, Yan-ju MA, Ru ZHENG, Jia-wei GUO, Lan-he ZHANG, authorCompany=null, correspAuthors=Yan-ping JIA, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1314917758370939555, articleId=1314917757913760417, tenantId=1146029695717560320, journalId=1234093305789726721, language=CN, title=利用小球藻（Chlorella vulgaris）评价Fe⁰/H₂O₂处理染料废水的生物毒性变化, columnId=1234106386565624579, journalTitle=中国环境科学, columnName=水污染与控制, runingTitle=null, highlight=null, articleAbstract=

通过分析小球藻的生长指标（生长率和抑制率）、光合作用指标（叶绿素a、叶绿素b、类胡萝卜素）、蛋白质含量、多糖含量和抗氧化酶活系统（超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、丙二醛（MDA））等指标的变化，以典型的三苯甲烷类染料（结晶紫）和吩嗪类染料（藏红T）作为研究对象，考察染料废水经Fe⁰/H₂O₂工艺处理前后的生物毒性变化．结果表明：随着反应时间延长，小球藻生长率下降，抑制率上升，染料废水原水显著抑制小球藻细胞的生长．与原水相比，处理后的出水中小球藻的叶绿素a升高22.01%，叶绿素b和类胡萝卜素含量分别降低12.97%和16.02%；SOD和CAT含量升高15.13%和12.5%，MDA含量降低23.97%．原水中小球藻的蛋白质含量较低，处理后出水中小球藻的蛋白质含量较高；与原水相比，出水中小球藻的多糖含量呈降低趋势．通过EPI Suite软件进行藻类毒性预测可知，结晶紫与藏红T降解的中间产物对鱼类、水蚤和绿藻的毒性明显降低．因此，Fe⁰/H₂O₂体系可有效降低染料废水的生物毒性．

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贾艳萍（1973-），女，吉林省吉林市人，教授，博士，主要从事废水处理理论与工艺的研究．发表论文70余篇．jiayanping1111@sina.com.

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贾艳萍（1973-），女，吉林省吉林市人，教授，博士，主要从事废水处理理论与工艺的研究．发表论文70余篇．jiayanping1111@sina.com.

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