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The granulation process and pollutant removal performance of aerobic granular sludge (AGS) under conventional and micro-nano aeration modes were investigated, with focus on the roles of extracellular polymeric substances (EPS) and microbial community succession in mediating microstructural responses. Compared to conventional aeration, the micro-nano aeration exhibited superior oxygen mass transfer efficiency and higher hydroxyl radical (·OH) generation, leading to significantly enhanced pollutant removal. Ammonia and chemical oxygen demand (COD) removal efficiencies consistently exceeded95%, while total nitrogen removal remained at a high level of 78.81%. Micro-nano aeration also accelerated AGS granulation, with mean particle sizes surpassing300 μm within20days. XDLVO-based interaction energy calculations indicated favorable aggregation behavior under both aeration regimes. Due to the biofilm-promoting effects of micro-nano aeration system, AGS developed contained higher EPS content under this mode, with Hydrophobic TB-PN playing a key role in forming protein-based hydrophobic networks that promoted AGS granulation. In contrast, AGS stability in the conventional system was primarily maintained via ionic bridging by Ionic TB-PS. Distinct microbial community structures were observed between the two aeration modes. Conventional aeration favored typical denitrifying bacteria, whereas the intensified of micro-nano aeration system led to a proliferation of filamentous taxa, notably Neomegalonema, Thiothrix, and norank_f_Saprospiraceae. These filamentous bacteria contributed to EPS secretion and AGS structural integrity while maintaining stable nitrogen removal performance.

, authors=Xin ZHANG¹, Kai-wen HUANG¹, Qian ZHAO¹, Li-wen XIAO¹, Shuo WANG^{1, 2, *}, Ji LI^{1, 2}, authorsList=Xin ZHANG, Kai-wen HUANG, Qian ZHAO, Li-wen XIAO, Shuo WANG, Ji LI, authorCompany=null, correspAuthors=Shuo WANG, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1314917733800706569, articleId=1314917731636445703, tenantId=1146029695717560320, journalId=1234093305789726721, language=CN, title=微纳米曝气促进好氧颗粒污泥形成的微观机制解析, columnId=1234106386565624579, journalTitle=中国环境科学, columnName=水污染与控制, runingTitle=null, highlight=null, articleAbstract=

通过研究常规曝气与微纳米曝气模式下好氧颗粒污泥（AGS）的颗粒化进程与污染物的去除效果，同时结合胞外聚合物（EPS）与微生物群落演替，分析不同曝气模式下AGS的微观响应特征．结果表明，相较于常规曝气模式，微纳米曝气体系具有更高的氧气传质效率与羟基自由基（·OH）产生量，可以显著提升AGS对污染物的去除效果，氨氮、化学需氧量（COD）的去除率始终稳定在95%以上，总氮去除率则能维持在78.81%的较高处理水平．微纳米曝气还可促进AGS的快速颗粒化，平均粒径在20d后超过300μm．且XDLVO理论计算结果表明两种曝气模式下AGS均具有良好的聚集性能．由于微纳米气泡具有促进生物膜生长的作用，微纳米曝气体系中的AGS具有含量更丰富的EPS，主要依靠Hydrophobic TB-PN形成蛋白质结构的疏水网络促进AGS形成，而常规曝气体系更多是依靠Ionic TB-PS的离子架桥作用增强AGS的稳定性．两种曝气体系中的微生物群落存在较大差异，常规曝气体系中多是常见的反硝化优势菌属，而微纳米曝气体系由于存在较高的曝气强度，导致丝状菌属出现了激增现象，优势菌群以Neomegalonema、Thiothrix和norank_f_Saprospiraceae为主，在为AGS分泌EPS形成骨架的同时，也能保证系统脱氮性能的稳定．

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张欣（2000-），女，江苏常州人，硕士研究生，主要研究方向为污水处理技术与好氧颗粒污泥研究．发表论文1篇．6221402053@stu.jiangnan.edu.cn.

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张欣（2000-），女，江苏常州人，硕士研究生，主要研究方向为污水处理技术与好氧颗粒污泥研究．发表论文1篇．6221402053@stu.jiangnan.edu.cn.

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