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Targeting the challenges in organic micropollutants (OMPs) detection and biotoxicity assessment, this study systematically evaluated four water pretreatment approaches and seven activated elution solvent combinations to improve the enrichment efficiency of residual organic matter in reclaimed water. The optimized procedure comprised: acidification to pH 2.5 with 5% (V/V) methanol addition; sequential SPE using HLB (500mg/6mL) and LC-18 (500mg/6mL) cartridges for broad-spectrum retention; ternary elution with 10mL methanol, 10mL ethyl acetate, and hexane (1:1V/V) to achieve polarity-balanced desorption. The optimized approach achieved 88% dissolved organic carbon (DOC) recovery, representing a 1.3~15.9-fold enhancement in OMPs recovery across compounds with logKow values ranging from 1.8 to 5.7 compared to conventional methanol-based methods. Notably, the optimized protocol reduced the sample volume required to reach EC50 in acute toxicity assays by 4.4-fold. The optimized method developed in this study significantly enhanced detection sensitivity and precision.

, authors=Yong-kun WANG1, Yu YAO2, Zhen LEI2, Xiao-yan MA2, Rong CHEN2, *, Xiao-chang WANG2, Guang-sheng ZHANG1, authorsList=Yong-kun WANG, Yu YAO, Zhen LEI, Xiao-yan MA, Rong CHEN, Xiao-chang WANG, Guang-sheng ZHANG, authorCompany=null, correspAuthors=Rong CHEN, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1314917725550510544, articleId=1314917723725988300, tenantId=1146029695717560320, journalId=1234093305789726721, language=CN, title=再生水残余有机物富集浓缩方法的优化与构建——以微量有机污染物和生物毒性检测为导向, columnId=1234106386565624579, journalTitle=中国环境科学, columnName=水污染与控制, runingTitle=null, highlight=null, articleAbstract=

以微量有机污染物(OMPs)和生物毒性检测为导向,系统考察4种水样预处理和7种不同活化洗脱溶剂组合对有机组分富集浓缩的影响,筛选并确立优化方法.结果表明,调节水样pH值为酸性(2.5)同时加入体积分数为5%的甲醇,活化洗脱溶剂组合为10mL甲醇、10mL乙酸乙酯和正己烷混合液(1:1),HLB(500mg/6mL)和LC-18(500mg/6mL)串联使用时,综合性指标DOC回收率可达88%;与传统甲醇方法相比,优化方法对10种不同logKow的OMPs回收效果均有提高,提高的倍数范围为1.3~15.9;获取的再生水有机物浓缩液用于发光细菌急性毒性检测分析时,生物毒性效应达到EC50所需要的水样体积可减少4.4倍,显著提高检测有效度和精度.

, authors=王永坤1, 姚宇2, 雷振2, 马晓妍2, 陈荣2, *, 王晓昌2, 张广胜1, authorsList=王永坤, 姚宇, 雷振, 马晓妍, 陈荣, 王晓昌, 张广胜, authorCompany=null, correspAuthors=陈荣, authorNote=

王永坤(1990-),安徽太和人,讲师,博士研究生,主要从事污水及再生水生态安全保障研究.发表论文10余篇..

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* 责任作者,教授,
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王永坤(1990-),安徽太和人,讲师,博士研究生,主要从事污水及再生水生态安全保障研究.发表论文10余篇..

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王永坤(1990-),安徽太和人,讲师,博士研究生,主要从事污水及再生水生态安全保障研究.发表论文10余篇..

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再生水残余有机物富集浓缩方法的优化与构建——以微量有机污染物和生物毒性检测为导向
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王永坤 1 , 姚宇 2 , 雷振 2 , 马晓妍 2 , 陈荣 2, * , 王晓昌 2 , 张广胜 1
中国环境科学 | 水污染与控制 2025,45(8): 4311-4321
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中国环境科学 |水污染与控制 2025 , 45 (8) : 4311 -4321
再生水残余有机物富集浓缩方法的优化与构建——以微量有机污染物和生物毒性检测为导向
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王永坤(1990-),安徽太和人,讲师,博士研究生,主要从事污水及再生水生态安全保障研究.发表论文10余篇..

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王永坤(1990-),安徽太和人,讲师,博士研究生,主要从事污水及再生水生态安全保障研究.发表论文10余篇..

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王永坤1 , 姚宇2, 雷振2, 马晓妍2, 陈荣2, * , 王晓昌2, 张广胜1
作者信息
  • 1.皖西学院环境与旅游学院,安徽 六安 237012
  • 2.西安建筑科技大学环境与市政工程学院,西北水资源与环境生态教育部重点实验室,陕西 西安 710055
通讯作者:
* 责任作者,教授,
作者简介:

王永坤(1990-),安徽太和人,讲师,博士研究生,主要从事污水及再生水生态安全保障研究.发表论文10余篇..

Optimization and development of enrichment protocols for residual organic matter in reclaimed water—targeting organic micropollutants and biotoxicity assessment
Yong-kun WANG1 , Yu YAO2, Zhen LEI2, Xiao-yan MA2, Rong CHEN2, * , Xiao-chang WANG2, Guang-sheng ZHANG1
Affiliations
  • 1.School of Environment and Tourism, West Anhui University, Lùan 237000, China
  • 2.Key Laboratory of Northwest Water Resource, Environment and Ecology, Ministry of Education, School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China
出版时间: 2025-08-20
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以微量有机污染物(OMPs)和生物毒性检测为导向,系统考察4种水样预处理和7种不同活化洗脱溶剂组合对有机组分富集浓缩的影响,筛选并确立优化方法.结果表明,调节水样pH值为酸性(2.5)同时加入体积分数为5%的甲醇,活化洗脱溶剂组合为10mL甲醇、10mL乙酸乙酯和正己烷混合液(1:1),HLB(500mg/6mL)和LC-18(500mg/6mL)串联使用时,综合性指标DOC回收率可达88%;与传统甲醇方法相比,优化方法对10种不同logKow的OMPs回收效果均有提高,提高的倍数范围为1.3~15.9;获取的再生水有机物浓缩液用于发光细菌急性毒性检测分析时,生物毒性效应达到EC50所需要的水样体积可减少4.4倍,显著提高检测有效度和精度.

再生水  /  固相萃取  /  溶解性有机物  /  微量有机污染物  /  生物毒性

Targeting the challenges in organic micropollutants (OMPs) detection and biotoxicity assessment, this study systematically evaluated four water pretreatment approaches and seven activated elution solvent combinations to improve the enrichment efficiency of residual organic matter in reclaimed water. The optimized procedure comprised: acidification to pH 2.5 with 5% (V/V) methanol addition; sequential SPE using HLB (500mg/6mL) and LC-18 (500mg/6mL) cartridges for broad-spectrum retention; ternary elution with 10mL methanol, 10mL ethyl acetate, and hexane (1:1V/V) to achieve polarity-balanced desorption. The optimized approach achieved 88% dissolved organic carbon (DOC) recovery, representing a 1.3~15.9-fold enhancement in OMPs recovery across compounds with logKow values ranging from 1.8 to 5.7 compared to conventional methanol-based methods. Notably, the optimized protocol reduced the sample volume required to reach EC50 in acute toxicity assays by 4.4-fold. The optimized method developed in this study significantly enhanced detection sensitivity and precision.

reclaimed water  /  solid phase extraction (SPE)  /  dissolved organic matter  /  organic micropollutants (OMPs)  /  biotoxicity
王永坤, 姚宇, 雷振, 马晓妍, 陈荣, 王晓昌, 张广胜. 再生水残余有机物富集浓缩方法的优化与构建——以微量有机污染物和生物毒性检测为导向. 中国环境科学, 2025 , 45 (8) : 4311 -4321 .
Yong-kun WANG, Yu YAO, Zhen LEI, Xiao-yan MA, Rong CHEN, Xiao-chang WANG, Guang-sheng ZHANG. Optimization and development of enrichment protocols for residual organic matter in reclaimed water—targeting organic micropollutants and biotoxicity assessment[J]. China Environmental Science, 2025 , 45 (8) : 4311 -4321 .
  • 安徽高校自然科学研究重点项目(KJ2021A0955)
  • 高层次人才科研项目(00701092228)
  • 国家杰出青年科学基金资助项目(52325002)
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  • 接收时间:2025-01-08
  • 首发时间:2026-10-08
  • 出版时间:2025-08-20
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  • 收稿日期:2025-01-08
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安徽高校自然科学研究重点项目(KJ2021A0955)
高层次人才科研项目(00701092228)
国家杰出青年科学基金资助项目(52325002)
作者信息
    1.皖西学院环境与旅游学院,安徽 六安 237012
    2.西安建筑科技大学环境与市政工程学院,西北水资源与环境生态教育部重点实验室,陕西 西安 710055

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2种不同金属材料的力学参数
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属
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鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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