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In this study, we investigated the transformation of sulfamethoxazole (SMX) by various reactive nitrogen intermediates (RNIs) (NO, NH₂OH, and free nitrous acid (FNA)), as well as the effect of FNA on the removal of SMX (1~5mg/L) by ammonia-oxidizing bacteria (AOB) under weak acidic conditions. The results demonstrated that FNA significantly enhanced the removal of SMX, with pH identified being the most influential factor. Under weak acidic conditions(pH=6~7), FNA-coupled AOB cometabolism achieved a SMX removal efficiency of 80.0% without significantly affecting ammonia oxidation efficiency. During the process, the content of extracellular polymers (EPS) exhibited an initial increase followed by a decrease. The content of ammonia monooxygenase (AMO) was slightly inhibited, while the levels of hydroxylamine oxidoreductase (HAO) initially decreased and then increased. Additionally, the abundance of the AOB genus Nitrosomonas decreased sharply from 56.4% to 0.7% before recovering to 14.7%. In contrast, the abundance of Comamonas and Thermomonas increased significantly, which helped maintain ammonia oxidation performance and SMX removal efficiency.

, authors=Zi-heng FENG¹, Bing-zheng WANG^{1, *}, Xiao-yu TIAN¹, Hao-xing HU¹, Jia-li FANG¹, Hui-yun ZHONG², Ji LI^{1, 3, 4}, authorsList=Zi-heng FENG, Bing-zheng WANG, Xiao-yu TIAN, Hao-xing HU, Jia-li FANG, Hui-yun ZHONG, Ji LI, authorCompany=null, correspAuthors=Bing-zheng WANG, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1314917664582103451, articleId=1314917664062009753, tenantId=1146029695717560320, journalId=1234093305789726721, language=CN, title=弱酸性条件游离亚硝酸耦合氨氧化细菌强化降解磺胺甲恶唑, columnId=1240689621210820752, journalTitle=中国环境科学, columnName=新污染物, runingTitle=null, highlight=null, articleAbstract=

探究了不同活性氮中间体（RNIs）（NO、NH₂OH、游离亚硝酸（FNA））对磺胺甲恶唑（SMX）的转化及弱酸性条件下FNA对氨氧化细菌（AOB）去除SMX（1~5mg/L）的影响．结果表明，FNA对SMX具有明显去除作用，pH值是最显著影响因素．弱酸性条件（pH=6~7）下，FNA耦合AOB共代谢对SMX的去除率达80.0%，且未对氨氧化效率产生显著影响；胞外聚合物（EPS）含量呈先升后降趋势；氨单加氧酶（AMO）含量受到轻微抑制，羟胺氧化还原酶（HAO）含量则先减后增；AOB菌属Nitrosomonas的丰度由56.4%降至0.7%，而后恢复至14.7%，Comamonas、Thermomonas的丰度也显著增加，有利于氨氧化性能的维持与SMX的去除．

, authors=冯梓恒¹, 王秉政^{1, *}, 田晓宇¹, 胡昊星¹, 方佳丽¹, 仲慧赟², 李激^{1, 3, 4}, authorsList=冯梓恒, 王秉政, 田晓宇, 胡昊星, 方佳丽, 仲慧赟, 李激, authorCompany=null, correspAuthors=王秉政, authorNote=, correspAuthorsNote=, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=f7L7m8oY9hFXIrPM6kQEgw==, magXml=dFWPvTIXEfdzHFM2duresQ==, pdfUrl=null, pdf=fKFXVvUDKitJlU1YZYNawA==, pdfFileSize=3557195, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=okHnuj2oTmZ1PO3LF0k9NQ==, mapNumber=null, fund=null)}, authors=[Author(id=1314917904953475677, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1314917664062009753, orderNo=0, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=1049241650@qq.com, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1314917905008001631, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1314917664062009753, authorId=1314917904953475677, language=EN, stringName=Zi-heng FENG, firstName=Zi-heng, middleName=null, lastName=FENG, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=¹, address=^1.School of Environment and Ecology, Jiangnan University, Wuxi 214122, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1314917905075110496, tenantId=1146029695717560320, journalId=1234093305789726721, articleId=1314917664062009753, authorId=1314917904953475677, language=CN, stringName=冯梓恒, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=¹, address=^1.江南大学环境与生态学院，江苏 无锡 214122, bio={"content":"

冯梓恒（2000-），男，浙江绍兴人，江南大学硕士研究生，研究方向为水处理中新兴污染物的处理．1049241650@qq.com.

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冯梓恒（2000-），男，浙江绍兴人，江南大学硕士研究生，研究方向为水处理中新兴污染物的处理．1049241650@qq.com.

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