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Developing a new method based on flow cytometry for rapid determination of the number of microplastics in drinking water, in response to the lack of rapid monitoring techniques that do not require visual sorting or pre-processing. The results showed that the use of Nile red dye (NR), methanol as the solvent, a dye concentration of 10μg/mL, a dyeing time of 10minutes, and flow cytometry for the quantitative analysis of microplastics in water, could reduce the testing time of a single sample to less than 14minutes. This method was used to analyse microplastics in influent and effluent water from a commercial bottled water plant and an ultrafiltration membrane. The results showed that the microplastic concentration in commercial bottled water was in the range (0.46~1.52)×105 particles/L. The newly used polyvinylidene fluoride (PVDF) ultrafiltration membrane posed a higher risk of releasing microplastics only during the initial filtration stage (1d), and the release of microplastics during long-term operation could be disregarded. This new membrane's microplastic release could be analysed with 99.99% efficiency and effectiveness. Additionally, it can efficiently and effectively remove 99.99% of microplastics from feed water.

, authors=Hai-xin BU1, Sen-lin SHAO2, Kai LI1, *, Gang WEN1, Ting-lin HUANG1, authorsList=Hai-xin BU, Sen-lin SHAO, Kai LI, Gang WEN, Ting-lin HUANG, authorCompany=null, correspAuthors=Kai LI, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1314917558805954847, articleId=1314917558197780765, tenantId=1146029695717560320, journalId=1234093305789726721, language=CN, title=流式细胞仪快速计量饮用水中微塑料数量, columnId=1234106386565624579, journalTitle=中国环境科学, columnName=水污染与控制, runingTitle=null, highlight=null, articleAbstract=

针对缺乏不需要视觉分类或预处理的快速监测技术问题,基于流式细胞仪开发可快速测定饮用水中微塑料数目的新方法.结果表明,选用尼罗红染料(NR),以甲醇作为的其溶剂、染料浓度选用10μg/mL、染色10min后,再采用流式细胞仪进行测定,可以实现水中微塑料的定量分析,单个样品的测试时间可以缩短到14min以内.应用该方法测定了商品瓶装水和超滤膜进出水的微塑料,结果表明商品瓶装水中微塑料浓度在(0.46~1.52)×105个/L.新使用的聚偏氟乙烯(PVDF)超滤膜仅在过滤初期(第1d)存在较高的微塑料释放风险,在长期运行中微塑料的释放可以忽略,另外其对进水中微塑料可以实现99.99%的高效去除.

, authors=步海鑫1, 邵森林2, 李凯1, *, 文刚1, 黄廷林1, authorsList=步海鑫, 邵森林, 李凯, 文刚, 黄廷林, authorCompany=null, correspAuthors=李凯, authorNote=

步海鑫(2000-),女,新疆乌鲁木齐人,西安建筑科技大学硕士研究生,主要从事超滤膜与微塑料污染研究..

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* 责任作者,教授,
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步海鑫(2000-),女,新疆乌鲁木齐人,西安建筑科技大学硕士研究生,主要从事超滤膜与微塑料污染研究..

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步海鑫(2000-),女,新疆乌鲁木齐人,西安建筑科技大学硕士研究生,主要从事超滤膜与微塑料污染研究..

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流式细胞仪快速计量饮用水中微塑料数量
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步海鑫 1 , 邵森林 2 , 李凯 1, * , 文刚 1 , 黄廷林 1
中国环境科学 | 水污染与控制 2025,45(7): 3807-3813
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中国环境科学 |水污染与控制 2025 , 45 (7) : 3807 -3813
流式细胞仪快速计量饮用水中微塑料数量
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步海鑫(2000-),女,新疆乌鲁木齐人,西安建筑科技大学硕士研究生,主要从事超滤膜与微塑料污染研究..

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步海鑫(2000-),女,新疆乌鲁木齐人,西安建筑科技大学硕士研究生,主要从事超滤膜与微塑料污染研究..

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步海鑫1 , 邵森林2, 李凯1, * , 文刚1, 黄廷林1
作者信息
  • 1.西安建筑科技大学环境与市政工程学院,陕西省环境工程重点实验室,陕西省水污染控制与水质安全保障协同创新中心,陕西 西安 710055
  • 2.武汉大学土木建筑工程学院,湖北 武汉 430072
通讯作者:
* 责任作者,教授,
作者简介:

步海鑫(2000-),女,新疆乌鲁木齐人,西安建筑科技大学硕士研究生,主要从事超滤膜与微塑料污染研究..

Rapid measurement of microplastics in drinking water by flow cytometry
Hai-xin BU1 , Sen-lin SHAO2, Kai LI1, * , Gang WEN1, Ting-lin HUANG1
Affiliations
  • 1. Shaanxi Key Laboratory of Environmental Engineering, Collaborative Innovation Center of Water Pollution Control and Water Quality Security Assurance of Shaanxi Province, School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China
  • 2.School of Civil Engineering, Wuhan University, Wuhan 430072, China
出版时间: 2025-07-20
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针对缺乏不需要视觉分类或预处理的快速监测技术问题,基于流式细胞仪开发可快速测定饮用水中微塑料数目的新方法.结果表明,选用尼罗红染料(NR),以甲醇作为的其溶剂、染料浓度选用10μg/mL、染色10min后,再采用流式细胞仪进行测定,可以实现水中微塑料的定量分析,单个样品的测试时间可以缩短到14min以内.应用该方法测定了商品瓶装水和超滤膜进出水的微塑料,结果表明商品瓶装水中微塑料浓度在(0.46~1.52)×105个/L.新使用的聚偏氟乙烯(PVDF)超滤膜仅在过滤初期(第1d)存在较高的微塑料释放风险,在长期运行中微塑料的释放可以忽略,另外其对进水中微塑料可以实现99.99%的高效去除.

微塑料  /  定量分析  /  流式细胞仪  /  瓶装水  /  超滤膜

Developing a new method based on flow cytometry for rapid determination of the number of microplastics in drinking water, in response to the lack of rapid monitoring techniques that do not require visual sorting or pre-processing. The results showed that the use of Nile red dye (NR), methanol as the solvent, a dye concentration of 10μg/mL, a dyeing time of 10minutes, and flow cytometry for the quantitative analysis of microplastics in water, could reduce the testing time of a single sample to less than 14minutes. This method was used to analyse microplastics in influent and effluent water from a commercial bottled water plant and an ultrafiltration membrane. The results showed that the microplastic concentration in commercial bottled water was in the range (0.46~1.52)×105 particles/L. The newly used polyvinylidene fluoride (PVDF) ultrafiltration membrane posed a higher risk of releasing microplastics only during the initial filtration stage (1d), and the release of microplastics during long-term operation could be disregarded. This new membrane's microplastic release could be analysed with 99.99% efficiency and effectiveness. Additionally, it can efficiently and effectively remove 99.99% of microplastics from feed water.

microplastics  /  quantitative analysis  /  flow cytometry  /  bottled water  /  ultrafiltration membrane
步海鑫, 邵森林, 李凯, 文刚, 黄廷林. 流式细胞仪快速计量饮用水中微塑料数量. 中国环境科学, 2025 , 45 (7) : 3807 -3813 .
Hai-xin BU, Sen-lin SHAO, Kai LI, Gang WEN, Ting-lin HUANG. Rapid measurement of microplastics in drinking water by flow cytometry[J]. China Environmental Science, 2025 , 45 (7) : 3807 -3813 .
  • 陕西省自然科学基金资助项目(2023-JC-YB-448)
  • 陕西省重点科技创新团队项目(2023-CX-TD-32)
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2025年第45卷第7期
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  • 接收时间:2024-12-18
  • 首发时间:2026-10-08
  • 出版时间:2025-07-20
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  • 收稿日期:2024-12-18
基金
陕西省自然科学基金资助项目(2023-JC-YB-448)
陕西省重点科技创新团队项目(2023-CX-TD-32)
作者信息
    1.西安建筑科技大学环境与市政工程学院,陕西省环境工程重点实验室,陕西省水污染控制与水质安全保障协同创新中心,陕西 西安 710055
    2.武汉大学土木建筑工程学院,湖北 武汉 430072

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2种不同金属材料的力学参数
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属
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鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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